9 results on '"Shan Mei"'
Search Results
2. Perfluorooctanoic acid in indoor particulate matter triggers oxidative stress and inflammation in corneal and retinal cells
- Author
-
Yun-Ping Lim, Jamie Jiin-Yi Chen, Shan-Mei Wu, Peng-Tai Tien, Chih Sheng Chen, Lei Wan, Hui Ju Lin, Chao-Jen Lin, Yi-Yu Tsai, Ching-Yao Chang, and Yuh-Jeen Huang
- Subjects
lcsh:Medicine ,Inflammation ,Retinal Pigment Epithelium ,010501 environmental sciences ,medicine.disease_cause ,01 natural sciences ,Article ,Retina ,Proinflammatory cytokine ,Environmental impact ,Cornea ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,medicine ,Humans ,lcsh:Science ,0105 earth and related environmental sciences ,Fluorocarbons ,Multidisciplinary ,Tight junction ,Chemistry ,Macular degeneration ,lcsh:R ,Endothelial Cells ,Retinal ,Epithelial Cells ,Molecular biology ,eye diseases ,Oxidative Stress ,medicine.anatomical_structure ,030221 ophthalmology & optometry ,Perfluorooctanoic acid ,lcsh:Q ,Particulate Matter ,sense organs ,medicine.symptom ,Caprylates ,Oxidative stress - Abstract
To investigate the particle size distribution of particulate matter and the concentration of specific perfluorinated compounds in indoor dust samples from several locations. Then, we used cell-based assays to investigate the effect of perfluorinated compounds on human corneal epithelial (HCEpiC), endothelial cells (HCEC) and retinal pigment epithelial cells (RPE). Indoor dust samples were collected at five different locations and PM50–10, PM10–2.5, and PM2.5–1 were fractionized. The presence and levels of 8:2 fluorotelomer alcohol, 10:2 fluorotelomer alcohol, and perfluorooctanoic acid were detected by gas chromatography–mass spectrometry. The effect of perfluorooctanoic acid on the activation of reactive oxygen species, transepithelial resistance as well as the expression of interleukin (IL)-6 and IL-8 were determined. The basolateral media of human corneal epithelial or human corneal endothelial cells were used to treat human corneal endothelial or retinal pigment epithelial cells, respectively to indicate the potential of ocular surface inflammation may result in retinal inflammation. Among perfluorinated compounds, only perfluorooctanoic acid was detected in all indoor dust samples. Perfluorooctanoic acid had the highest concentration among all perfluorinated compounds in the samples. Exposure to perfluorooctanoic acid impaired tight junction sealing and increased the levels of reactive oxygen species in human corneal epithelial cells. In human corneal epithelial cells, secretion of IL-6 and IL-8 in both apical and basolateral media was promoted significantly by perfluorooctanoic acid treatment. Stimulation with the basolateral media from perfluorooctanoic acid-treated human corneal epithelial cells induced inflammation in human corneal endothelial cells. The treatment of retinal pigment epithelial cells with the basolateral media from stimulated human corneal endothelial cells also elicited the secretion of proinflammatory cytokines. The results indicate that perfluorooctanoic acid exposure impaired the tight junction of corneal cells and caused inflammatory reactions in the retina. Exposure of the cornea to perfluorooctanoic acid contained in particulate matter might induce oxidative stress and inflammation in the retina and represent a risk factor for age-related macular degeneration.
- Published
- 2020
3. Altered resting-state brain activity in Parkinson’s disease patients with freezing of gait
- Author
-
Tao Wu, Peipeng Liang, Linlin Gao, Piu Chan, Tao-Mian Mi, Shan-Shan Mei, and Kuncheng Li
- Subjects
Male ,0301 basic medicine ,medicine.medical_specialty ,Cerebellum ,Parkinson's disease ,genetic structures ,Brain activity and meditation ,Rest ,Thalamus ,lcsh:Medicine ,Brain mapping ,Article ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Basal ganglia ,Humans ,Medicine ,Author Correction ,lcsh:Science ,Gait ,Gait Disorders, Neurologic ,Aged ,Brain Mapping ,Multidisciplinary ,Resting state fMRI ,business.industry ,Putamen ,lcsh:R ,Brain ,Parkinson Disease ,Middle Aged ,medicine.disease ,Magnetic Resonance Imaging ,030104 developmental biology ,medicine.anatomical_structure ,nervous system ,Case-Control Studies ,Cardiology ,Female ,lcsh:Q ,business ,human activities ,030217 neurology & neurosurgery - Abstract
Freezing of gait (FOG) is a common and debilitating symptom in Parkinson’s disease (PD). The current study investigated alterations of resting-state spontaneous brain activity in PD patients with FOG. A total of 29 patients with FOG, 28 patients without FOG and 31 controls were included. All subjects underwent resting-state functional MRI, and the amplitude of low-frequency fluctuation (ALFF) was calculated to measure the spontaneous brain activity. Between-group differences and correlations with FOG severity (both subjective and objective measures) were analyzed. Compared to those without FOG, patients with FOG showed increased ALFF in right anterior cingulate cortex (ACC) and left inferior parietal lobule (IPL), as well as decreased ALFF in right superior frontal gyrus (SFG), bilateral cerebellum and left thalamus. Correlation analyses demonstrated that ALFF within the right SFG, right ACC and bilateral pallidum were positively correlated with FOG; while ALFF within the thalamus, putamen, cerebellum and sensorimotor regions were negatively correlated. Our results indicate that FOG is associated with dysfunction within frontal-parietal regions, along with increased inhibitory outputs from basal ganglia. Additionally, altered activity of cerebellum implicates its role in the pathophysiology of FOG. These findings provide further insight into the underlying neural mechanisms of FOG in PD patients.
- Published
- 2017
4. Amphotericin B Inhibits Enterovirus 71 Replication by Impeding Viral Entry
- Author
-
Shan Mei, Shan Cen, Jianwei Wang, Fengwen Xu, Lijuan Yin, Siqi Hu, Lili Ren, Qi Jin, Fei Guo, Zhendong Zhao, Chen Liang, Jian Li, Bin Ai, Ying Wang, Tingting Liu, and Xiaoxiao Zhao
- Subjects
0301 basic medicine ,medicine.drug_class ,media_common.quotation_subject ,030106 microbiology ,Antibiotics ,Virus Replication ,Article ,Microbiology ,03 medical and health sciences ,Viral entry ,Amphotericin B ,Cell Line, Tumor ,Enterovirus Infections ,medicine ,Enterovirus 71 ,Humans ,Internalization ,media_common ,Multidisciplinary ,biology ,HEK 293 cells ,Virus Internalization ,biology.organism_classification ,Virology ,Enterovirus A, Human ,030104 developmental biology ,Viral replication ,Cell culture ,medicine.drug - Abstract
Enterovirus 71 (EV71) infection causes hand-foot-and-mouth disease that leads to cardiopulmonary complications and death in young children. There is thus an urgent need to find new treatments to control EV71 infection. In this study, we report potent inhibition of EV71 by a polyene antibiotic Amphotericin B. Amphotericin B profoundly diminished the expression of EV71 RNA and viral proteins in the RD cells and the HEK293 cells. As a result, EV71 production was inhibited by Amphotericin B with an EC50 (50% effective concentration) of 1.75 μM in RD cells and 0.32 μM in 293 cells. In addition to EV71, EV68 was also strongly inhibited by Amphotericin B. Results of mechanistic studies revealed that Amphotericin B targeted the early stage of EV71 infection through impairing the attachment and internalization of EV71 by host cells. As an effective anti-fungi drug, Amphotericin B thus holds the promise of formulating a novel therapeutic to treat EV71 infection.
- Published
- 2016
5. Identification of a nanobody specific to human pulmonary surfactant protein A
- Author
-
Meng-Meng Zhao, Jianhua He, Feng Yu, Xian He, Nan Li, Ya-Ru Wei, Yu-Kui Du, Yan Li, Liu-Sheng Wang, Zhao Fang Yin, Dong Weng, Hui-Ping Li, Yang Hu, Shan-Shan Chen, Shan-Shan Du, and Shan-Mei Wang
- Subjects
Pathology ,medicine.medical_specialty ,Science ,Mice, Nude ,Article ,law.invention ,Cell Line ,03 medical and health sciences ,0302 clinical medicine ,Drug Delivery Systems ,law ,medicine ,Animals ,Humans ,Tissue Distribution ,Lung ,A549 cell ,Kidney ,Mice, Inbred BALB C ,Multidisciplinary ,biology ,Pulmonary Surfactant-Associated Protein A ,HEK 293 cells ,Single-Domain Antibodies ,Molecular biology ,Recombinant Proteins ,Rats ,medicine.anatomical_structure ,030228 respiratory system ,Targeted drug delivery ,030220 oncology & carcinogenesis ,Toxicity ,biology.protein ,Recombinant DNA ,Medicine ,Female ,Erratum ,Protein A - Abstract
Nanobody (Nb) is a promising vector for targeted drug delivery. This study aims to identify an Nb that can specifically target the lung by binding human pulmonary surfactant protein A (SP-A). Human lung frozen tissue sections were used for 3 rounds of biospanning of our previously constructed Nb library for rat SP-A to establish a sub-library of Nb, which specifically bound human lung tissues. Phage-ELISA was performed to screen the sub-library to identify Nb4, which specifically bound human SP-A. The binding affinity Kd of Nb4 to recombinant human SP-A was 7.48 × 10−7 M. Nb4 (19 kDa) was stable at 30 °C–37 °C and pH 7.0–7.6 and specifically bound the SP-A in human lung tissue homogenates, human lung A549 cells, and human lung tissues, whereas didn’t react with human liver L-02 cells, kidney 293T cells, and human tissues from organs other than the lung. Nb4 accumulated in the lung of nude mice 5 minutes after a tail vein injection of Nb4 and was excreted 3 hours. Short-term exposure (one month) to Nb4 didn’t cause apparent liver and kidney toxicity in rats, whereas 3-month exposure resulted in mild liver and kidney injuries. Nb4 may be a promising vector to specifically deliver drugs to the lung.
- Published
- 2016
6. Author Correction: Altered resting-state brain activity in Parkinson’s disease patients with freezing of gait
- Author
-
Piu Chan, Kuncheng Li, Tao Wu, Peipeng Liang, Tao-Mian Mi, Linlin Gao, and Shan-Shan Mei
- Subjects
medicine.medical_specialty ,Multidisciplinary ,Parkinson's disease ,Resting state fMRI ,Brain activity and meditation ,business.industry ,lcsh:R ,lcsh:Medicine ,medicine.disease ,Gait (human) ,Physical medicine and rehabilitation ,ComputingMethodologies_DOCUMENTANDTEXTPROCESSING ,Medicine ,lcsh:Q ,business ,lcsh:Science - Abstract
A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper.
- Published
- 2018
7. Erratum: Identification of a nanobody specific to a human pulmonary surfactant protein A
- Author
-
He, Xian, primary, Wang, Shan-Mei, additional, Yin, Zhao Fang, additional, Zhao, Meng-Meng, additional, Li, Nan, additional, Yu, Feng, additional, Wang, Liu-Sheng, additional, Hu, Yang, additional, Du, Yu-Kui, additional, Du, Shan-Shan, additional, Li, Yan, additional, Wei, Ya-Ru, additional, Chen, Shan-Shan, additional, He, Jian-Hua, additional, Weng, Dong, additional, and Li, Hui-Ping, additional
- Published
- 2017
- Full Text
- View/download PDF
8. Identification of a nanobody specific to human pulmonary surfactant protein A
- Author
-
He, Xian, primary, Wang, Shan-Mei, additional, Fang Yin, Zhao, additional, Zhao, Meng-Meng, additional, Li, Nan, additional, Yu, Feng, additional, Wang, Liu-Sheng, additional, Hu, Yang, additional, Du, Yu-Kui, additional, Du, Shan-Shan, additional, Li, Yan, additional, Wei, Ya-Ru, additional, Chen, Shan-Shan, additional, He, Jian-Hua, additional, Weng, Dong, additional, and Li, Hui-Ping, additional
- Published
- 2017
- Full Text
- View/download PDF
9. Identification of a nanobody specific to human pulmonary surfactant protein A
- Author
-
Xian He, Shan-Mei Wang, Zhao Fang Yin, Meng-Meng Zhao, Nan Li, Feng Yu, Liu-Sheng Wang, Yang Hu, Yu-Kui Du, Shan-Shan Du, Yan Li, Ya-Ru Wei, Shan-Shan Chen, Jian-Hua He, Dong Weng, and Hui-Ping Li
- Subjects
Medicine ,Science - Abstract
Abstract Nanobody (Nb) is a promising vector for targeted drug delivery. This study aims to identify an Nb that can specifically target the lung by binding human pulmonary surfactant protein A (SP-A). Human lung frozen tissue sections were used for 3 rounds of biospanning of our previously constructed Nb library for rat SP-A to establish a sub-library of Nb, which specifically bound human lung tissues. Phage-ELISA was performed to screen the sub-library to identify Nb4, which specifically bound human SP-A. The binding affinity Kd of Nb4 to recombinant human SP-A was 7.48 × 10−7 M. Nb4 (19 kDa) was stable at 30 °C–37 °C and pH 7.0–7.6 and specifically bound the SP-A in human lung tissue homogenates, human lung A549 cells, and human lung tissues, whereas didn’t react with human liver L-02 cells, kidney 293T cells, and human tissues from organs other than the lung. Nb4 accumulated in the lung of nude mice 5 minutes after a tail vein injection of Nb4 and was excreted 3 hours. Short-term exposure (one month) to Nb4 didn’t cause apparent liver and kidney toxicity in rats, whereas 3-month exposure resulted in mild liver and kidney injuries. Nb4 may be a promising vector to specifically deliver drugs to the lung.
- Published
- 2017
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.