31 results on '"Yanyan Li"'
Search Results
2. Elevated PDE4C level serves as a candidate diagnostic biomarker and correlates with poor survival in thyroid carcinoma
- Author
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Ying Wang, Yongsheng Zhang, Yanyan Li, and Jing Huang
- Subjects
Phosphodiesterase 4 ,Thyroid carcinoma ,Cyclic-adenosine monophosphate ,Biomarker ,Prognosis ,Medicine ,Science - Abstract
Abstract Thyroid carcinoma (THCA) is the most common endocrine cancer. Phosphodiesterase (PDE) 4 enzyme family, as specific regulator of cyclic adenosine monophosphate, may play a important role in THCA. However, few studies on PDE4 enzyme family in THCA have been reported yet. Therefore, this study aimed to systematically analyze the changes of PDE4 enzyme family in THCA, and look for potential target for THCA therapy. We systematically analyzed the expression differences, prognostic value, genetic alteration, methylation modification, and the correlation with tumor immune microenvironment of PDE4 family in THCA using several public databases, including TCGA, GEO, GSCA, TNMplot, cBioPortal, DiseaseMeth and TIMER. Besides, functional enrichment analysis and protein–protein interaction (PPI) network of PDE4 family was investigated using Metascape and STRING databases. The expression levels of PDE4A, PDE4B and PDE4D were down-regulated in THCA patients at different cancer stages, while the expression level of PDE4C was significantly up-regulated. Moreover, THCA patients with higher PDE4C expression had shorter progress free survival compared with those with lower PDE4C expression. The low genomic alteration frequencies and mildly increased methylation levels of PDE4 family were found in THCA patients. Except for PDE4A, the expression levels of PDE4B, PDE4C and PDE4D could affect many immune cells infiltration during THCA progression. Four PDE4 subtypes were all enriched in cAMP catabolic process. Nevertheless, PDE4C was not enriched in the cAMP binding signal pathway, and PDE4B was not enriched in the G alphas signaling events. Notably, PDE4C participated in cAMP metabolic process by regulating adenylate cyclases (ADCYs), which involved ADCY1, ADCY5, ADCY6, ADCY8 and ADCY9. The findings of this study provide a partial basis for the role of PDE4 family in the occurrence and development of THCA. In addition, this study also suggested that PDE4C might be a potential prognostic marker of THCA, which could serve as a reference for future basic and clinical research.
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- 2024
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- View/download PDF
3. Author Correction: Skiing economy and kinematic during a field double poling roller skiing among novice and experienced cross-country skiers
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Yang Zhu, Zhiqiang Wang, Ruoyang Li, Yanyan Li, Peng Bai, Weifeng Gao, and Yaping Zhong
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Medicine ,Science - Published
- 2024
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4. Integrative analysis reveals the functional implications and clinical relevance of pyroptosis in low-grade glioma
- Author
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Lin Shen, Yanyan Li, Na Li, Yajie Zhao, Qin Zhou, Liangfang Shen, and Zhanzhan Li
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Medicine ,Science - Abstract
Abstract Using the Chinese Glioma Genome Atlas (training dataset) and The Cancer Genome Atlas (validation dataset), we found that low-grade gliomas can be divided into two molecular subclasses based on 30 pyroptosis genes. Cluster 1 presented higher immune cell and immune function scores and poorer prognosis than Cluster 2. We established a prognostic model based on 10 pyroptosis genes; the model could predict overall survival in glioma and was well validated in an independent dataset. The high-risk group had relatively higher immune cell and immune function scores and lower DNA methylation levels in pyroptosis genes than the low-risk group. There were no marked differences in pyroptosis gene alterations between the high- and low-risk groups. The competing endogenous RNA (ceRNA) regulatory network uncovered the lncRNA–miRNA–mRNA regulation patterns of the different risk groups in low-grade glioma. Five pairs of target genes and drugs were identified. In vitro, CASP8 silencing inhibited the migration and invasion of glioma cells. The expression of pyroptosis genes can reflect the molecular biological and clinical features of low-grade glioma subclasses. The developed prognostic model can predict overall survival and distinguish molecular alterations in patients. Our integrated analyses could provide valuable guidelines for improving risk management and therapy for low-grade glioma patients.
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- 2022
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- View/download PDF
5. Integrated biocontrol of tobacco bacterial wilt by antagonistic bacteria and marigold
- Author
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Yun Hu, Wan Zhao, Xihong Li, Ji Feng, Chunli Li, Xiaoqiong Yang, Qingqing Guo, Lin Wang, Shouwen Chen, Yanyan Li, and Yong Yang
- Subjects
Medicine ,Science - Abstract
Abstract Tobacco bacterial wilt (TBW) is seriously damages the growth of tobacco. There is an urgent need to find a safer and more effective measure to control TBW. In this study, B. amyloliquefaciens ZM9 and marigold powder were applied to the tobacco roots alone or in combination, and the potential inhibition of TBW was assessed. On the other hand, the effects of these treatments on soil physicochemical properties, rhizosphere microbial community and soil metabolites were also evaluated. The results showed that the application of B. amyloliquefaciens ZM9 or marigold powder alone significantly reduced the abundance of R. solanacearum in rhizosphere soil, while the integrated treatment showed the strongest inhibitory effect. Moreover, the integrated treatment can inhibit the secretion of chemoattractants, and affect the change of rhizosphere soil microbial composition. In conclusion, the combination of antagonistic bacteria agent B. amyloliquefaciens ZM9 with marigold powder can enhance the suppression of TBW. Furthermore, B. amyloliquefaciens ZM9 and marigold have synergistic effects on suppressing TBW by regulation soil physicochemical properties, soil metabolites and microbial structure. This study provide a promising strategy for TBW control by integrated applying of B. amyloliquefaciens ZM9 and marigold powder.
- Published
- 2021
- Full Text
- View/download PDF
6. Digital gene expression analysis of the response to Ralstonia solanacearum between resistant and susceptible tobacco varieties
- Author
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YanYan Li, Lin Wang, GuangWei Sun, XiHong Li, ZhenGuo Chen, Ji Feng, and Yong Yang
- Subjects
Medicine ,Science - Abstract
Abstract Tobacco bacterial wilt (TBW) caused by Ralstonia solanacearum is the most serious soil-borne disease of tobacco. However, molecular mechanism information of R. solanacearum resistance is limited to tobacco, hindering better breeding of resistant tobacco. In this study, the expression profiles of the rootstalks of Yunyan87 (susceptible cultivar) and Fandi3 (resistant cultivar) at different stages after R. solanacearum infection were compared to explore molecular mechanisms of tobacco resistance against the bacterium. Findings from gene-expression profiling indicated that the number of upregulated differentially expressed genes (DEGs) at 3 and 7 days post-inoculation (dpi) increased significantly in the resistant cultivar. WRKY6 and WRKY11 family genes in WRKY transcription factors, ERF5 and ERF15 family genes in ERFs transcription factors, and genes encoding PR5 were significantly upregulated in the resistant cultivar response to the infection. For the first time, WRKY11 and ERF15 were found to be possibly involved in disease-resistance. The Kyoto Encyclopedia of Genes and Genomes analysis demonstrated glutathione metabolism and phenylpropane pathways as primary resistance pathways to R. solanacearum infection. In the resistant cultivar, DEGs encoding CYP450, TCM, CCoAOMT, 4CL, PAL, CCR, CSE, and CADH, involved in the synthesis of plant antitoxins such as flavonoids, stilbenoids, and lignins, enriched in the phenylpropane pathway were upregulated at 3 and 7 dpi. Furthermore, a pot experiment was performed to verify the role of flavonoids in controlling TBW. This study will strongly contribute to a better understanding of molecular interactions between tobacco plants and R. solanacearum.
- Published
- 2021
- Full Text
- View/download PDF
7. Effects of integrated biocontrol on bacterial wilt and rhizosphere bacterial community of tobacco
- Author
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Yun Hu, Yanyan Li, Xiaoqiong Yang, Chunli Li, Lin Wang, Ji Feng, Shouwen Chen, Xihong Li, and Yong Yang
- Subjects
Medicine ,Science - Abstract
Abstract Bacterial wilt as a soil-borne disease was caused by Ralstonia solanacearum, and seriously damages the growth of tobacco. Integrated biocontrol method was explored to control bacterial wilt. Nevertheless, the long-term effects of the integrated biocontrol method on soil bacterial community, soil physicochemical properties and the incidence of bacterial wilt are not well understood. In this study, B. amyoliquefaciens ZM9, calcium cyanamide and rice bran were applied to tobacco fields in different ways. The disease index and incidence of tobacco bacterial wilt (TBW), soil physicochemical properties, colonization ability of B. amyoliquefaciens ZM9, and rhizopshere bacterial community were investigated. The results showed that the integrated application of B. amyoliquefaciens ZM9, rice bran and calcium cyanamide had the highest control efficiency of TBW and bacteria community diversity. Additionally, the integrated biocontrol method could improve the colonization ability of B. amyoliquefaciens ZM9. Furthermore, the integrated biocontrol method could effectively suppress TBW by regulating soil physicochemical properties, promoting beneficial bacteria and antagonistic bacteria of rhizopshere soil. This strategy has prospect of overcoming the defects in application of a single antagonistic bacteria and provides new insights to understand how to improve the colonization capacity of antagonistic bacteria and control efficacy for TBW.
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- 2021
- Full Text
- View/download PDF
8. Geometric Heterogeneity of Continental Shale in the Yanchang Formation, Southern Ordos Basin, China
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Lihui Li, Beixiu Huang, Yufang Tan, Xiaolong Deng, Yanyan Li, and Hu Zheng
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Medicine ,Science - Abstract
Abstract Favorable prospects for the exploration of shale gas have been demonstrated in the Ordos Basin, China. Outcrop and core observations indicate that there are abundant laminas in the shale strata, which exert a great influence on hydro-fracture propagation, gas storage and fluid flow. In this study, the continental shale of the Chang 72 Member, collected from the south of Ordos Basin, was investigated to characterize the geometric heterogeneity. Laminas at multiple scales were observed and measured using conventional logging, borehole TV, core analysis, scanning electron microscopy, and the Particle and Crack Analysis System. These measurement tools correspond to the meter scale, decimeter scale, centimeter scale, millimeter scale and ten-micrometer scale, respectively, with measured thicknesses of 2.26 m, 2.09 dm, 1.70 cm, 1.48 mm and 11.70 μm, respectively. Fractal theory was used to analyze the power exponent distribution of the lamina thickness, with a resulting fractal dimension of 1.06. Finally, a geometric heterogeneity model was proposed for the Upper Triassic Yanchang Formation in the study area and verified by a modeled thickness of 26.30 m for the Chang 72 Member at the 10-m scale. The model facilitates cross-scale analysis and provides parameter guidance for heterogeneity characterization in the numerical simulation and model test of the shale gas reservoir.
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- 2017
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9. A high-fat diet impairs reproduction by decreasing the IL1β level in mice treated at immature stage
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Jie Zhang, kai Li, Miao Yuan, Guizen Huang, Jie Ao, Haoze Tan, Yanyan Li, Di Gong, Jun Li, lei Kang, Nini An, Fei Li, Ping Lin, and Lugang Huang
- Subjects
Medicine ,Science - Abstract
Abstract Obesity causes low-grade inflammation that is involved in male infertility. Interleukin 1 beta (IL1β) plays an important role in this process. A high-fat diet (HFD) is the most common cause of obesity. However, the effect of a HFD on IL1β and its consequence in reproduction remain unclear. We established a HFD model in mice treated at immature stage (mice-TIS) and mice treated at mature stage (mice-TMS). Surprisingly, we found that a HFD decreased IL1β levels and was accompanied by an increase in testosterone in mice-TIS, while the reverse results were observed in mice-TMS. In addition, a HFD caused a reduction in testis macrophages and in the expression of inflammasome-related genes and proteins in mice-TIS. Furthermore, we found that IL1β inhibited testosterone secretion through down-regulating the gene expression of P450SCC and P450c17. However, the influence on mice-TIS that were induced by a HFD was recovered by stopping the HFD. In this study, we are the first to report that a HFD impairs the reproductive system by decreasing IL1β and enhancing testosterone levels in mice-TIS, which are different from the effects in mice-TMS. This provides new ideas for the treatment of obesity-induced infertility.
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- 2017
- Full Text
- View/download PDF
10. Integrated biocontrol of tobacco bacterial wilt by antagonistic bacteria and marigold
- Author
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Wang Lin, Yang Xiaoqiong, Guo Qingqing, Shouwen Chen, Zhao Wan, Xihong Li, Yong Yang, Li Chunli, Yun Hu, Feng Ji, and Yanyan Li
- Subjects
Science ,Biological pest control ,Bacillus ,Microbial communities ,Plant Roots ,Article ,Applied microbiology ,Soil ,fluids and secretions ,Tobacco ,Food science ,Inhibitory effect ,Soil Microbiology ,Plant Diseases ,Rhizosphere ,Multidisciplinary ,biology ,Chemistry ,Microbiota ,Bacterial wilt ,Microbial composition ,biology.organism_classification ,Biological Control Agents ,Microbial population biology ,Medicine ,Powders ,Bacteria - Abstract
Tobacco bacterial wilt (TBW) is seriously damages the growth of tobacco. There is an urgent need to find a safer and more effective measure to control TBW. In this study, B. amyloliquefaciens ZM9 and marigold powder were applied to the tobacco roots alone or in combination, and the potential inhibition of TBW was assessed. On the other hand, the effects of these treatments on soil physicochemical properties, rhizosphere microbial community and soil metabolites were also evaluated. The results showed that the application of B. amyloliquefaciens ZM9 or marigold powder alone significantly reduced the abundance of R. solanacearum in rhizosphere soil, while the integrated treatment showed the strongest inhibitory effect. Moreover, the integrated treatment can inhibit the secretion of chemoattractants, and affect the change of rhizosphere soil microbial composition. In conclusion, the combination of antagonistic bacteria agent B. amyloliquefaciens ZM9 with marigold powder can enhance the suppression of TBW. Furthermore, B. amyloliquefaciens ZM9 and marigold have synergistic effects on suppressing TBW by regulation soil physicochemical properties, soil metabolites and microbial structure. This study provide a promising strategy for TBW control by integrated applying of B. amyloliquefaciens ZM9 and marigold powder.
- Published
- 2021
11. Digital gene expression analysis of the response to Ralstonia solanacearum between resistant and susceptible tobacco varieties
- Author
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Feng Ji, Yanyan Li, Chen Zhenguo, Wang Lin, Sun Guangwei, Xihong Li, and Yong Yang
- Subjects
0106 biological sciences ,0301 basic medicine ,Science ,01 natural sciences ,Article ,Plant breeding ,03 medical and health sciences ,Species Specificity ,Gene expression ,Tobacco ,Cluster Analysis ,Cultivar ,KEGG ,Transcription factor ,Gene ,Disease Resistance ,Plant Diseases ,Genetics ,Ralstonia solanacearum ,Multidisciplinary ,biology ,Bacterial wilt ,Gene Expression Profiling ,fungi ,food and beverages ,biology.organism_classification ,WRKY protein domain ,030104 developmental biology ,Plant stress responses ,Host-Pathogen Interactions ,Medicine ,Plant sciences ,010606 plant biology & botany ,Transcription Factors - Abstract
Tobacco bacterial wilt (TBW) caused by Ralstonia solanacearum is the most serious soil-borne disease of tobacco. However, molecular mechanism information of R. solanacearum resistance is limited to tobacco, hindering better breeding of resistant tobacco. In this study, the expression profiles of the rootstalks of Yunyan87 (susceptible cultivar) and Fandi3 (resistant cultivar) at different stages after R. solanacearum infection were compared to explore molecular mechanisms of tobacco resistance against the bacterium. Findings from gene-expression profiling indicated that the number of upregulated differentially expressed genes (DEGs) at 3 and 7 days post-inoculation (dpi) increased significantly in the resistant cultivar. WRKY6 and WRKY11 family genes in WRKY transcription factors, ERF5 and ERF15 family genes in ERFs transcription factors, and genes encoding PR5 were significantly upregulated in the resistant cultivar response to the infection. For the first time, WRKY11 and ERF15 were found to be possibly involved in disease-resistance. The Kyoto Encyclopedia of Genes and Genomes analysis demonstrated glutathione metabolism and phenylpropane pathways as primary resistance pathways to R. solanacearum infection. In the resistant cultivar, DEGs encoding CYP450, TCM, CCoAOMT, 4CL, PAL, CCR, CSE, and CADH, involved in the synthesis of plant antitoxins such as flavonoids, stilbenoids, and lignins, enriched in the phenylpropane pathway were upregulated at 3 and 7 dpi. Furthermore, a pot experiment was performed to verify the role of flavonoids in controlling TBW. This study will strongly contribute to a better understanding of molecular interactions between tobacco plants and R. solanacearum.
- Published
- 2021
12. Effects of integrated biocontrol on bacterial wilt and rhizosphere bacterial community of tobacco
- Author
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Yang Xiaoqiong, Li Chunli, Yun Hu, Xihong Li, Yong Yang, Shouwen Chen, Feng Ji, Yanyan Li, and Wang Lin
- Subjects
0301 basic medicine ,Science ,030106 microbiology ,Biological pest control ,Microbial communities ,Article ,03 medical and health sciences ,chemistry.chemical_compound ,Bacillus amyloliquefaciens ,Tobacco ,Colonization ,Microbe ,Pest Control, Biological ,Plant Diseases ,Rhizosphere ,Ralstonia solanacearum ,Multidisciplinary ,biology ,Bran ,business.industry ,Bacterial wilt ,Calcium cyanamide ,food and beverages ,biology.organism_classification ,Biotechnology ,030104 developmental biology ,Soil microbiology ,chemistry ,Medicine ,business ,Bacteria - Abstract
Bacterial wilt as a soil-borne disease was caused by Ralstonia solanacearum, and seriously damages the growth of tobacco. Integrated biocontrol method was explored to control bacterial wilt. Nevertheless, the long-term effects of the integrated biocontrol method on soil bacterial community, soil physicochemical properties and the incidence of bacterial wilt are not well understood. In this study, B. amyoliquefaciens ZM9, calcium cyanamide and rice bran were applied to tobacco fields in different ways. The disease index and incidence of tobacco bacterial wilt (TBW), soil physicochemical properties, colonization ability of B. amyoliquefaciens ZM9, and rhizopshere bacterial community were investigated. The results showed that the integrated application of B. amyoliquefaciens ZM9, rice bran and calcium cyanamide had the highest control efficiency of TBW and bacteria community diversity. Additionally, the integrated biocontrol method could improve the colonization ability of B. amyoliquefaciens ZM9. Furthermore, the integrated biocontrol method could effectively suppress TBW by regulating soil physicochemical properties, promoting beneficial bacteria and antagonistic bacteria of rhizopshere soil. This strategy has prospect of overcoming the defects in application of a single antagonistic bacteria and provides new insights to understand how to improve the colonization capacity of antagonistic bacteria and control efficacy for TBW.
- Published
- 2020
13. Agronomic evaluation of polymer-coated urea and urease and nitrification inhibitors for cotton production under drip-fertigation in a dry climate
- Author
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Mingfang Hu, Zhiwen Ma, Xiangyi Li, Fanjiang Zeng, Xiaopeng Gao, Yanyan Li, Mario Tenuta, and Dongwei Gui
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0106 biological sciences ,Fertigation ,Urease ,lcsh:Medicine ,Growing season ,Drip irrigation ,01 natural sciences ,Article ,Environmental impact ,chemistry.chemical_compound ,lcsh:Science ,Multidisciplinary ,biology ,Crop yield ,lcsh:R ,Sowing ,04 agricultural and veterinary sciences ,Agronomy ,chemistry ,Coated urea ,040103 agronomy & agriculture ,Urea ,biology.protein ,0401 agriculture, forestry, and fisheries ,Environmental science ,lcsh:Q ,Agroecology ,010606 plant biology & botany - Abstract
Interest in the use of enhanced-efficiency nitrogen (N) fertilizers (EENFs) has increased in recent years due to their potential to increase crop yield and reduce environmental N loss. Drip-fertigation is widely used for crop production in arid regions to improve water and nutrient use efficiency whereas the effectiveness of EENFs with drip irrigation remains unclear. A field experiment was conducted in 2015 and 2016 to examine the effects of EENFs on yield, N use and quality of cotton (Gossypium hirsutum) grown under drip-fertigation in arid NW China. Treatments included an unfertilized control and application of 240 kg N ha−1 by polymer-coated urea (ESN), urea alone, or urea plus urease (NBPT) and nitrification (DCD) inhibitors. ESN was all banded in the plant row at planting, whereas urea was applied with 20% N banded at planting and 80% N by six fertigation events over the growing season. Results showed there was generally no treatment effect on seed and lint yield, N concentration or allocations, N recovery efficiency and fiber quality index of cotton. A lack of treatment effect could be due to N supplied with drip-fertigation better synthesized with crop N needs and the relatively high soil native NO3− availability, which hindered the effect of polymer-coated urea and double inhibitors. These results highlight the challenge of the employment of EENFs products for drip-fertigation system in arid area. Further research is required to define the field conditions under which the agronomic efficiency of EENFs products may be achieved in accordance with weather conditions.
- Published
- 2020
14. Proteomic Analysis Reveals Dab2 Mediated Receptor Endocytosis Promotes Liver Sinusoidal Endothelial Cell Dedifferentiation
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Handong Wei, Bintao Qiu, Yanyan Li, Weiran Lin, Yuanxiang Lao, Huahai Chen, Yufang Hou, Fuchu He, Aihua Sun, and Ying Jiang
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Male ,Proteomics ,0301 basic medicine ,Proteome ,Liver cytology ,Angiogenesis ,lcsh:Medicine ,Receptors, Cell Surface ,Cell Separation ,Endocytosis ,Article ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Western blot ,medicine ,Animals ,Receptor ,lcsh:Science ,Adaptor Proteins, Signal Transducing ,Multidisciplinary ,medicine.diagnostic_test ,Immunomagnetic Separation ,Chemistry ,lcsh:R ,Computational Biology ,Endothelial Cells ,Reproducibility of Results ,Cell Dedifferentiation ,Cell biology ,Endothelial stem cell ,Adaptor Proteins, Vesicular Transport ,030104 developmental biology ,Liver ,030211 gastroenterology & hepatology ,lcsh:Q ,Apoptosis Regulatory Proteins ,Intracellular ,Isobaric tag for relative and absolute quantitation - Abstract
Sinusoidal dedifferentiation is a complicated process induced by several factors, and exists in early stage of diverse liver diseases. The mechanism of sinusoidal dedifferentiation is poorly unknown. In this study, we established a NaAsO2-induced sinusoidal dedifferentiation mice model. Liver sinusoidal endothelial cells were isolated and isobaric tag for relative and absolute quantitation (iTRAQ) based proteomic approach was adopted to globally examine the effects of arsenic on liver sinusoidal endothelial cells (LSECs) during the progression of sinusoidal dedifferentiation. In all, 4205 proteins were identified and quantified by iTRAQ combined with LC-MS/MS analysis, of which 310 proteins were significantly changed in NaAsO2 group, compared with the normal control. Validation by western blot showed increased level of clathrin-associated sorting protein Disabled 2 (Dab2) in NaAsO2 group, indicating that it may regulate receptor endocytosis, which served as a mechanism to augment intracellular VEGF signaling. Moreover, we found that knockdown of Dab2 reduced the uptake of VEGF in LSECs, furthermore blocking VEGF-mediated LSEC dedifferentiation and angiogenesis.
- Published
- 2017
15. Comprehensive analyses of fatty acid metabolism-related lncRNA for ovarian cancer patients
- Author
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Min Li, Ye Yan, Yanyan Liu, Jianzhen Zhao, Fei Guo, Jianqin Chen, Lifang Nie, Yong Zhang, and Yingmei Wang
- Subjects
Medicine ,Science - Abstract
Abstract Ovarian cancer (OC) is a disease with difficult early diagnosis and treatment and poor prognosis. OC data profiles were downloaded from The Cancer Genome Atlas. Eight key fatty acid metabolism-related long non-coding RNAs (lncRNAs) were finally screened for building a risk scoring model by univariate/ multifactor and least absolute shrinkage and selection operator (LASSO) Cox regression. To make this risk scoring model more applicable to clinical work, we established a nomogram containing the clinical characteristics of OC patients after confirming that the model has good reliability and validity and the ability to distinguish patient prognosis. To further explore how these key lncRNAs are involved in OC progression, we explored their relationship with LUAD immune signatures and tumor drug resistance. The structure shows that the risk scoring model established based on these 8 fatty acid metabolism-related lncRNAs has good reliability and validity and can better predict the prognosis of patients with different risks of OC, and LINC00861in these key RNAs may be a hub gene that affects the progression of OC and closely related to the sensitivity of current OC chemotherapy drugs. In addition, combined with immune signature analysis, we found that patients in the high-risk group are in a state of immunosuppression, and Tfh cells may play an important role in it. We innovatively established a prognostic prediction model with excellent reliability and validity from the perspective of OC fatty acid metabolism reprogramming and lncRNA regulation and found new molecular/cellular targets for future OC treatment.
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- 2023
- Full Text
- View/download PDF
16. Discovery and control of culturable and viable but non-culturable cells of a distinctive Lactobacillus harbinensis strain from spoiled beer
- Author
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Yanyan Li, Brian M. Peters, Shishui Zhou, Bing Li, Junyan Liu, Lin Li, Zhenbo Xu, Mark E. Shirtliff, and Yang Deng
- Subjects
0301 basic medicine ,030106 microbiology ,Food spoilage ,lcsh:Medicine ,Lactobacillus harbinensis ,Article ,03 medical and health sciences ,chemistry.chemical_compound ,Food science ,lcsh:Science ,Cells, Cultured ,Multidisciplinary ,Microbial Viability ,biology ,business.industry ,lcsh:R ,food and beverages ,Beer ,biology.organism_classification ,Lactic acid ,Lactobacillus ,Gene Ontology ,chemistry ,Genes, Bacterial ,Brewing ,lcsh:Q ,business ,Bacteria - Abstract
Occasional beer spoilage incidents caused by false-negative isolation of lactic acid bacteria (LAB) in the viable but non-culturable (VBNC) state, result in significant profit loss and pose a major concern in the brewing industry. In this study, both culturable and VBNC cells of an individual Lactobacillus harbinensis strain BM-LH14723 were identified in one spoiled beer sample by genome sequencing, with the induction and resuscitation of VBNC state for this strain further investigated. Formation of the VBNC state was triggered by low-temperature storage in beer (175 ± 1.4 days) and beer subculturing (25 ± 0.8 subcultures), respectively, and identified by both traditional staining method and PMA-PCR. Resuscitated cells from the VBNC state were obtained by addition of catalase rather than temperature upshift, changing medium concentration, and adding other chemicals, and both VBNC and resuscitated cells retained similar beer-spoilage capability as exponentially growing cells. In addition to the first identification of both culturable and VBNC cells of an individual L. harbinensis strain from spoiled beer, this study also for the first time reported the VBNC induction and resuscitation, as well as verification of beer-spoilage capability of VBNC and resuscitated cells for the L. harbinensis strain. Genes in association with VBNC state were also identified by the first genome sequencing of beer spoilage L. harbinensis. The results derived from this study suggested the contamination and spoilage of beer products by VBNC and resuscitated L. harbinensis strain BM-LH14723.
- Published
- 2018
17. An orthogonal system for heterologous expression of actinobacterial lasso peptides in Streptomyces hosts
- Author
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Jimmy Mevaere, Olha Schneider, Haiyan Ma, Carlos Afonso, Sylvie Rebuffat, Christophe Goulard, Yanyan Li, Séverine Zirah, Olga N. Sekurova, Sergey B. Zotchev, Laboratoire de chimie et biochimie des substances naturelles, Muséum national d'Histoire naturelle (MNHN)-Centre National de la Recherche Scientifique (CNRS), Norwegian University of Science and Technology [Trondheim] (NTNU), Department of Pharmacognosy, University of Vienna [Vienna], Institut des Sciences Chimiques de Rennes (ISCR), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes-Centre National de la Recherche Scientifique (CNRS), Chimie Organique et Bioorganique : Réactivité et Analyse (COBRA), Institut de Chimie Organique Fine (IRCOF), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut Normand de Chimie Moléculaire Médicinale et Macromoléculaire (INC3M), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Université Le Havre Normandie (ULH), Normandie Université (NU)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS), Centre de pharmacologie et innovation dans le diabète (CPID), Centre National de la Recherche Scientifique (CNRS)-Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)-Université Montpellier 1 (UM1), Molécules de Communication et Adaptation des Micro-organismes (MCAM), Norwegian University of Science and Technology (NTNU), Institut Normand de Chimie Moléculaire Médicinale et Macromoléculaire (INC3M), Institut de Chimie du CNRS (INC)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Institut de Chimie du CNRS (INC)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie Organique Fine (IRCOF), and Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
0301 basic medicine ,[SDV.BIO]Life Sciences [q-bio]/Biotechnology ,Chemical biology ,lcsh:Medicine ,Heterologous ,Computational biology ,Microbial Sensitivity Tests ,Biology ,Actinoalloteichus ,Genome ,Streptomyces ,Article ,03 medical and health sciences ,Lasso (statistics) ,Bacterial Proteins ,Tandem Mass Spectrometry ,Amino Acid Sequence ,lcsh:Science ,Peptide sequence ,Natural products ,Multidisciplinary ,lcsh:R ,biology.organism_classification ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,Anti-Bacterial Agents ,Actinobacteria ,030104 developmental biology ,Genes, Bacterial ,Multigene Family ,lcsh:Q ,Heterologous expression ,Peptides - Abstract
Lasso peptides are ribosomally synthesized and post-translationally modified peptides produced by bacteria. They are characterized by an unusual lariat-knot structure. Targeted genome scanning revealed a wide diversity of lasso peptides encoded in actinobacterial genomes, but cloning and heterologous expression of these clusters turned out to be problematic. To circumvent this, we developed an orthogonal expression system for heterologous production of actinobacterial lasso peptides in Streptomyces hosts based on a newly-identified regulatory circuit from Actinoalloteichus fjordicus. Six lasso peptide gene clusters, mainly originating from marine Actinobacteria, were chosen for proof-of-concept studies. By varying the Streptomyces expression hosts and a small set of culture conditions, three new lasso peptides were successfully produced and characterized by tandem MS. The newly developed expression system thus sets the stage to uncover and bioengineer the chemo-diversity of actinobacterial lasso peptides. Moreover, our data provide some considerations for future bioprospecting efforts for such peptides. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Cre- ative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not per- mitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ © The Author(s) 2018
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- 2018
18. Prognostic significances of overexpression MYC and/or BCL2 in R-CHOP-treated diffuse large B-cell lymphoma: A Systematic review and meta-analysis
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Lu Li, Ximei Que, Kai-Li Ma, Tong Wang, Yanyan Li, Xue Gao, Mingxing Yu, Qian Gao, and Yanfeng Xi
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0301 basic medicine ,Oncology ,Male ,medicine.medical_specialty ,Vincristine ,lcsh:Medicine ,Article ,Proto-Oncogene Proteins c-myc ,03 medical and health sciences ,Antibodies, Monoclonal, Murine-Derived ,0302 clinical medicine ,immune system diseases ,Internal medicine ,hemic and lymphatic diseases ,Antineoplastic Combined Chemotherapy Protocols ,medicine ,Humans ,lcsh:Science ,B-cell lymphoma ,Cyclophosphamide ,Survival analysis ,Multidisciplinary ,business.industry ,lcsh:R ,Hazard ratio ,medicine.disease ,Prognosis ,Survival Analysis ,Lymphoma ,030104 developmental biology ,Proto-Oncogene Proteins c-bcl-2 ,Doxorubicin ,030220 oncology & carcinogenesis ,Meta-analysis ,Prednisone ,lcsh:Q ,Rituximab ,Lymphoma, Large B-Cell, Diffuse ,business ,Diffuse large B-cell lymphoma ,medicine.drug - Abstract
Numerous studies have investigated the prognostic values of MYC and/or BCL2 protein overexpression in diffuse large B-cell lymphoma (DLBCL). However, the results still demonstrate discrepancies among different studies. We aimed to do a systematic review and meta-analysis on the relationships between overexpression MYC and/or BCL2 and DLBCLs treated with rituximab, cyclophosphamide, doxorubicin, vincristine, and prednisone (R-CHOP). This study followed the guidelines of PRISMA and Cochrane handbook. The hazard ratios (HRs) for overall survival (OS) were pooled to estimate the main effect size. Twenty studies recruited a total of 5576 patients were available for this meta-analysis. The results showed that MYC (HR = 1.96, 95%CI (confidence interval) = 1.69–2.27)without heterogeneity(I2 = 17.2%, P = 0.280), BCL2 (HR = 1.65, 95%CI = 1.43–1.89, I2 = 20.7%, P = 0.234) protein overexpression, and co-overexpression (HR = 2.58, 95%CI = 2.19–3.04, I2 = 17.2%, P = 0.275) had a poor prognosis in R-CHOP treated DLBCL patients, respectively. The current analysis indicated that MYC and/or BCL2 protein overexpression, and particularly co-overexpression was related to short overall survival in R-CHOP treated DLBCL patients, showing that application of the two new biomarkers can help to better stratify DLBCL patients and guide targeted treatment.
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- 2018
19. Understanding the high l-valine production in Corynebacterium glutamicum VWB-1 using transcriptomics and proteomics
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Yanyan Li, Hailing Zhang, Xiaoyuan Wang, and Chenhui Wang
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Proteomics ,0301 basic medicine ,030106 microbiology ,lcsh:Medicine ,Pentose phosphate pathway ,Article ,Corynebacterium glutamicum ,Transcriptome ,03 medical and health sciences ,Leucine ,Ribosomal protein ,Pyruvic Acid ,lcsh:Science ,Gene ,Alanine ,Multidisciplinary ,Chemistry ,lcsh:R ,Valine ,Elongation factor ,030104 developmental biology ,Biochemistry ,Proteome ,lcsh:Q ,NADP - Abstract
Toward the elucidation of the advanced mechanism of l-valine production by Corynebacterium glutamicum, a highly developed industrial strain VWB-1 was analyzed, employing the combination of transcriptomics and proteomics methods. The transcriptional level of 1155 genes and expression abundance of 96 proteins were changed significantly by the transcriptome and proteome comparison of VWB-1 and ATCC 13869. It was indicated that the key genes involved in the biosynthesis of l-valine, ilvBN, ilvC, ilvD, ilvE were up-regulated in VWB-1, which together made prominent contributions in improving the carbon flow towards l-valine. The l-leucine and l-isoleucine synthesis ability were weakened according to the down-regulation of leuB and ilvA. The up-regulation of the branched chain amino acid transporter genes brnFE promoted the l-valine secretion capability of VWB-1. The NADPH and ATP generation ability of VWB-1 were strengthened through the up-regulation of the genes involved in phosphate pentose pathway and TCA pathway. Pyruvate accumulation was achieved through the weakening of the l-lactate, acetate and l-alanine pathways. The up-regulation of the genes coding for elongation factors and ribosomal proteins were beneficial for l-valine synthesis in C. glutamicum. All information acquired were useful for the genome breeding of better industrial l-valine producing strains.
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- 2018
20. Toxicology evaluation of overdose hydroxychloroquine on zebrafish (Danio rerio) embryos
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Min Luo, Dan Xie, Ziyuan Lin, Huaqin Sun, and Yanyan Liu
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Medicine ,Science - Abstract
Abstract Potential risks of treatment with hydroxychloroquine (HCQ) include QT interval prolongation, hypoglycemia, a wide range of neuropsychiatric manifestations, hematotoxicity, and potential genetic defects. HCQ is extremely toxic when used in overdose and can lead to tachycardia, hypotension, known central nervous system, transmission defects, hypokalemia and other manifestations in individuals. The mechanism of excessive HCQ leading to these manifestations is still unclear. In this paper, overdose HCQ at different concentrations was used to treat zebrafish embryos, and the phenomena like human beings were obtained, such as increased heart rate and nervous system inhibition. With the increase of concentration to 100 μM, embryo mortality and malformation rate increased and hatching rate decreased, in situ hybridization showed abnormal differentiation of embryo germ layers and formation of vital organs. We selected embryos treated with 50 μM HCQ, in which concentration the mortality rate, hatching rate and malformation rate of the embryos were like those of the control group, for transcriptome analysis. Although the above indexes did not change significantly, the molecular changes related to the development of the heart, eye, nerve and other important organs were significant. This study provides useful information for further research on the toxicity mechanism of HCQ overdose, and provides some insight that can guide future studies in humans.
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- 2022
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21. IL-6 mediates differentiation disorder during spermatogenesis in obesity-associated inflammation by affecting the expression of Zfp637 through the SOCS3/STAT3 pathway
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Guizhen Huang, Miao Yuan, Jie Zhang, Jun Li, Di Gong, Yanyan Li, Ping Lin, and Lugang Huang
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0301 basic medicine ,Leptin ,Male ,STAT3 Transcription Factor ,medicine.medical_specialty ,Cell signaling ,Cellular differentiation ,Down-Regulation ,Mice, Obese ,Stat3 Signaling Pathway ,Article ,Cell Line ,03 medical and health sciences ,Mice ,0302 clinical medicine ,Downregulation and upregulation ,Internal medicine ,Testis ,medicine ,Animals ,SOCS3 ,Obesity ,Phosphorylation ,STAT3 ,Spermatogenesis ,Inflammation ,030219 obstetrics & reproductive medicine ,Multidisciplinary ,biology ,Interleukin-6 ,SOXB1 Transcription Factors ,Tyrphostins ,Spermatogonia ,DNA-Binding Proteins ,030104 developmental biology ,Endocrinology ,Suppressor of Cytokine Signaling 3 Protein ,biology.protein ,RNA Interference ,Signal transduction ,Signal Transduction - Abstract
Zfp637 is a recently identified zinc finger protein and its functions remain largely unknown. Here, we innovatively demonstrate the effects of Zfp637 on the differentiation of mouse spermatogonia and on its downstream target gene SOX2 in vitro. Obesity has been recognized as a chronic inflammatory disease that leads to decreased sexual function and sexual development disorders. We observed higher levels of IL-6 in serum and testis homogenates from obese mice compared with control mice. We also demonstrated that high levels of IL-6 inhibited Zfp637 expression and we elucidated the underlying mechanisms. SOCS3 overexpression and STAT3 phosphorylation inhibitor (AG490) were used to investigate the function of the SOCS3/STAT3 pathway during this process. Our results showed that exposure of mouse spermatogonial cells to high levels of IL-6 inhibited Zfp637 expression by increasing SOCS3 expression and inhibiting the phosphorylation of STAT3, further reducing cellular differentiation. Consistent with the in vitro results, we observed increasing expression levels of SOCS3 and SOX2, but a reduction of Zfp637 expression, in obese mouse testes. In conclusion, Zfp637 plays a crucial role in spermatogenesis by downregulating SOX2 expression and IL-6 can decrease the expression of Zfp637 through the SOCS3/STAT3 signaling pathway.
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- 2016
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22. Anemia increases the mortality risk in patients with stroke: A meta-analysis of cohort studies
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Peng Chen, Lizhang Chen, Zhanzhan Li, Yanyan Li, and Tao Zhou
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Male ,medicine.medical_specialty ,Funnel plot ,Anemia ,030204 cardiovascular system & hematology ,Article ,Disease-Free Survival ,Cohort Studies ,03 medical and health sciences ,0302 clinical medicine ,Risk Factors ,Internal medicine ,medicine ,Humans ,Stroke ,Multidisciplinary ,business.industry ,Odds ratio ,Publication bias ,medicine.disease ,Confidence interval ,Surgery ,Survival Rate ,Meta-analysis ,Female ,business ,030217 neurology & neurosurgery ,Cohort study - Abstract
The impact of anemia on the outcome of patients with stroke remains inconsistent. We performed a meta-analysis of cohort studies to assess the mortality risk in stroke patients with and without anemia. Systematic searches were conducted in the PubMed, China National Knowledge Infrastructure, Web of Science and Wanfang databases to identify relevant studies from inception to November 2015. The estimated odds ratio with a 95% confidence interval was pooled. subgroup analyses and sensitivity analyses were also conducted. We used Begg’s funnel plot and Egger’s test to detect the potential publication bias. Thirteen cohort studies with a total of 19239 patients with stroke were included in this meta-analysis. The heterogeneity among studies was slight (I2 = 59.0%, P = 0.031). The results from a random-effect model suggest that anemia is associated with an increased mortality risk in patients with stroke (adjusted odds ratio = 1.39, 95% confidence interval: 1.22–1.58, P
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- 2016
23. Differentiating T2 hyperintensity in neonatal white matter by two-compartment model of diffusional kurtosis imaging
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Lingxia Zeng, Qinli Sun, Xianjun Li, Bolang Yu, Chao Jin, Jian Yang, Yanyan Li, Duan Xu, and Jie Gao
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Pathology ,medicine.medical_specialty ,Encephalopathy ,Article ,White matter ,03 medical and health sciences ,0302 clinical medicine ,030225 pediatrics ,Image Interpretation, Computer-Assisted ,medicine ,Humans ,Brain Diseases ,Multidisciplinary ,medicine.diagnostic_test ,Cerebral white matter ,business.industry ,Compartment (ship) ,Brain ,Infant ,Magnetic resonance imaging ,medicine.disease ,Magnetic Resonance Imaging ,White Matter ,Hyperintensity ,medicine.anatomical_structure ,nervous system ,Kurtosis ,business ,030217 neurology & neurosurgery ,Infant, Premature ,Diffusion MRI - Abstract
In conventional neonatal MRI, the T2 hyperintensity (T2h) in cerebral white matter (WM) at term-equivalent age due to immaturity or impairment is still difficult to identify. To clarify such issue, this study used the metrics derived from a two-compartment WM model of diffusional kurtosis imaging (WM-DKI), including intra-axonal, extra-axonal axial and radial diffusivities (Da, De,// and De,⊥), to compare WM differences between the simple T2h and normal control for both preterm and full-term neonates, and between simple T2h and complex T2h with hypoxic-ischemic encephalopathy (HIE). Results indicated that compared with control, the simple T2h showed significantly increased De,// and De,⊥, but no significant change in Da in multiple premyelination regions, indicative of expanding extra-axonal diffusion microenvironment; while myelinated regions showed no changes. However, compared with simple T2h, the complex T2h with HIE had decreased Da, increased De,⊥ in both premyelination and myelinated regions, indicative of both intra- and extra-axonal diffusion alterations. While diffusion tensor imaging (DTI) failed to distinguish simple T2h from complex T2h with HIE. In conclusion, superior to DTI-metrics, WM-DKI metrics showed more specificity for WM microstructural changes to distinguish simple T2h from complex T2h with HIE.
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- 2016
24. X-ray Irradiated Vaccine Confers protection against Pneumonia caused by Pseudomonas Aeruginosa
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Yuquan Wei, Xiaoxiao Liu, J. P. Tang, Zhenling Wang, Bin Peng, and Yanyan Li
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CD4-Positive T-Lymphocytes ,Cytotoxicity, Immunologic ,Lipopolysaccharides ,0301 basic medicine ,Cellular immunity ,Adoptive cell transfer ,Neutrophils ,Heterologous ,Virulence ,Biology ,medicine.disease_cause ,Article ,Microbiology ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Antigen ,medicine ,Animals ,Pseudomonas Infections ,Antiserum ,Microbial Viability ,Multidisciplinary ,Pseudomonas aeruginosa ,X-Rays ,Antibodies, Bacterial ,Virology ,Bacterial Load ,Bacterial vaccine ,Disease Models, Animal ,030104 developmental biology ,Vaccines, Inactivated ,Bacterial Vaccines ,Th17 Cells ,Female ,Immunization ,030215 immunology - Abstract
Pseudomonas aeruginosa is a gram-negative bacterium and one of the leading causes of nosocomial infection worldwide, however, no effective vaccine is currently available in the market. Here, we demonstrate that inactivation of the bacteria by X-ray irradiation inhibits its replication capability but retained antigenic expression functionally thus allowing its use as a potential vaccine. Mice immunized by this vaccine were challenged by the parental strain, the O-antigen-homologous strain PAO-1 (O2/O5) and heterologous strain PAO-6 (O6) in an acute pneumonia model. We further measured the protective effect of the vaccine, as well as host innate and cellular immunity responses. We found immunized mice could protect against both strains. Notably, the antiserum only had significant protective role against similar bacteria, while adoptive transfer of lymphocytes significantly controlled the spread of the virulent heterologous serogroup PAO-6 infection and the protective role could be reversed by CD4 rather than CD8 antibody. We further revealed that vaccinated mice could rapidly recruit neutrophils to the airways early after intranasal challenge by PAO-6 and the irradiated vaccine was proved to be protective by the generated CD4+ IL-17+ Th17 cells. In conclusion, the generation of inactivated but metabolically active microbes is a promising strategy for safely vaccinating against Pseudomonas aeruginosa.
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- 2016
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25. ZNF32 inhibits autophagy through the mTOR pathway and protects MCF-7 cells from stimulus-induced cell death
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Le Zhang, Ping Lin, Kai Li, Yan Xu, Yuquan Wei, Hongjiang Li, Yuyan Wei, Jie Zhang, Junping Gao, Yanyan Li, Jun Li, and Rong Xiang
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Programmed cell death ,Cell ,Kruppel-Like Transcription Factors ,Gene Expression ,Breast Neoplasms ,Biology ,Article ,Phagosomes ,Autophagy ,medicine ,Animals ,Humans ,Protein kinase B ,PI3K/AKT/mTOR pathway ,Diamide ,Multidisciplinary ,Cell growth ,TOR Serine-Threonine Kinases ,RPTOR ,Hydrogen Peroxide ,Cell biology ,Disease Models, Animal ,medicine.anatomical_structure ,Gene Knockdown Techniques ,MCF-7 Cells ,Cancer research ,Heterografts ,Female ,Signal transduction ,Proto-Oncogene Proteins c-akt ,Signal Transduction - Abstract
ZNF32 is a recently identified zinc finger protein and its functions remain largely unknown. Autophagy has been shown to affect cell proliferation and survival. Here, we innovatively show the effect of ZNF32 on cell autophagy and autophagy-associated cell death in breast carcinoma cells and also elucidate its underlying mechanisms. We examined the autophagic activity and LC3 II expression in human carcinoma cell lines with increased or decreased ZNF32 expression. Pharmacological inhibition (rapamycin) or activation (EGF) assays were used to investigate the function of the AKT/mTOR pathway during this process. H2O2- and diamide-induced MCF-7 cell death models were used to elucidate the role of ZNF32-associated autophagy in breast carcinoma cell death. Our results show that increasing ZNF32 expression in MCF-7 cells inhibits autophagy initiation by activating the AKT/mTOR pathway and further reduced autophagy-associated cell death and maintained MCF-7 cell survival. Conversely, impairing ZNF32 expression by transfecting ZNF32 siRNA strongly promoted autophagy, further augmenting autophagy-associated cell death. Furthermore, correlations between ZNF32 and autophagy were observed in both MCF-7 xenograft tumors and in breast cancer patients. In conclusion, ZNF32 acts as an effective autophagy inhibitor to protect breast cancer cells from excessive stimulus-autophagy-induced cell death.
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- 2015
26. Establishment and characterization of patient-derived tumor xenograft using gastroscopic biopsies in gastric cancer
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Lai Wang, Yan Zhu, Jianling Zou, Bin Dong, Zhongwu Li, Yanyan Li, Na Li, Zhiyu Tang, Yilin Li, Jian Wu, Lin Shen, Tiantian Tian, and Jing Gao
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Adult ,Male ,Pathology ,medicine.medical_specialty ,medicine.medical_treatment ,Transplantation, Heterologous ,Mice, SCID ,Nod ,Article ,Mice ,Mice, Inbred NOD ,Stomach Neoplasms ,In vivo ,Gastroscopy ,Tumor Cells, Cultured ,medicine ,Animals ,Humans ,Pathological ,Aged ,Cell Proliferation ,Aged, 80 and over ,Severe combined immunodeficiency ,Chemotherapy ,Multidisciplinary ,business.industry ,Cancer ,Middle Aged ,medicine.disease ,Transplantation ,Disease Models, Animal ,Latency stage ,Female ,business - Abstract
The patient-derived tumor xenograft (PDTX) model has become the most realistic model for preclinical studies. PDTX models of gastric cancer using surgical tissues are reported occasionally; however, the PDTX models using gastroscopic biopsies, which are best for evaluating new drugs, are unreported. In our study, a total of 185 fresh gastroscopic biopsies of gastric cancer were subcutaneously transplanted into NOD/SCID (Nonobese Diabetic/Severe Combined Immunodeficiency) mice. Sixty-three PDTX models were successfully established (34.1%, 63/185) and passaged to maintain tumors in vivo and the mean latency period of xenografts was 65.86 ± 32.84 days (11–160 days). Biopsies of prior chemotherapy had a higher transplantation rate (52.1%, 37/71) than biopsies after chemotherapy (21.9%, 25/114; P = 0.000). No differences were found between the latency period of xenografts and characteristics of patients. The pathological and molecular features of PDTX as well as chemosensitivity were highly consistent with those of primary tumors of patients. The genetic characteristics were stable during passaging of PDTX models. In summary PDTX models using gastroscopic biopsies in gastric cancer were demonstrated for the first time and the biological characteristics of the PDTX models were highly consistent with patients, which provided the best preclinical study platform for gastric cancer.
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- 2015
27. Relationship between red cell distribution width and serum uric acid in patients with untreated essential hypertension
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Yanyan Li, Zhanzhan Li, Shipeng Yan, Lizhang Chen, Peng Chen, Yingyun Hu, and Min Luo
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Male ,medicine.medical_specialty ,Erythrocytes ,Cross-sectional study ,Essential hypertension ,Article ,chemistry.chemical_compound ,Internal medicine ,Humans ,Medicine ,Blood urea nitrogen ,Triglycerides ,Retrospective Studies ,Multidisciplinary ,business.industry ,Incidence (epidemiology) ,Red blood cell distribution width ,Retrospective cohort study ,Odds ratio ,Middle Aged ,medicine.disease ,Uric Acid ,Cross-Sectional Studies ,Endocrinology ,chemistry ,Hypertension ,Erythrocyte Count ,Uric acid ,Female ,Essential Hypertension ,business - Abstract
We assessed whether red cell distribution width (RDW) is associated with serum uric acid (UA) level in a group of 512 patients with newly diagnosed hypertension, recruited in Beijing. Patients were divided into high uric acid group and low uric acid group according to the median (334.9 μmol/L) of serum uric acid. Compared with the low uric acid group, the patients with high uric acid had higher red blood cell count (P < 0.001) and RDW (P = 0.032). The multiple linear regression analysis showed that RDW (P = 0.001) was positively correlated with uric acid level after the adjustment of related factors. Stepwise multiple logistic regression model confirmed that RDW (odds ratio: OR = 1.75) was independent determinants of high serum uric acid as well as sex (OR = 6.03), triglycerides (OR = 1.84), and Blood Urea Nitrogen (BUN, OR = 1.30). RDW may be independently associated with serum UA level in patients with newly diagnosed hypertension. To firmly establish the causal role of RDW in the incidence of high uric acid level among hypertensive patients, large cohort studies are needed.
- Published
- 2014
28. Feasibility of reduced-dose posttransplant cyclophosphamide and cotransplantation of peripheral blood stem cells and umbilical cord-derived mesenchymal stem cells for SAA
- Author
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Yingling Zu, Jian Zhou, Yuewen Fu, Baijun Fang, Xinjian Liu, Yanli Zhang, Fengkuan Yu, Wenli Zuo, Hu Zhou, Ruirui Gui, Zhen Li, Yanyan Liu, Huifang Zhao, Chengjuan Zhang, and Yongping Song
- Subjects
Medicine ,Science - Abstract
Abstract Posttransplant cyclophosphamide (PTCy) as graft-versus-host disease (GVHD) prophylaxis is an effective strategie for patients receiving matched sibling donor hematopoietic stem cell transplantation (MSD-HSCT) and haploidentical HSCT (haplo-HSCT). We evaluated the effectiveness and safety of reduced-dose cyclophosphamide, 20 mg/kg for 13 patients in MSD-HSCT cohort and 25 mg/kg for 22 patients in haplo-HSCT cohort, on days + 3, + 4 combined with cotransplantation of peripheral blood stem cells (PBSCs) and human umbilical cord-derived mesenchymal stem cells (UC-MSCs) for severe aplastic anemia (SAA). In MSD-PTCy cohort, the times to neutrophil and platelet engraftment were significantly shorter than those in the MSD-control cohort (P
- Published
- 2021
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29. An optimal BMI range associated with a lower risk of mortality among HIV-infected adults initiating antiretroviral therapy in Guangxi, China
- Author
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Junjun Jiang, Xionglin Qin, Huifang Liu, Sirun Meng, Abu S. Abdullah, Jinping Huang, Chunwei Qin, Yanfen Liu, Yunxuan Huang, Fengxiang Qin, Jiegang Huang, Ning Zang, Bingyu Liang, Chuanyi Ning, Yanyan Liao, Hao Liang, Fengyao Wu, and Li Ye
- Subjects
Medicine ,Science - Abstract
Abstract Previous studies investigating HIV-infected patients suggested a direct link between underweight and the mortality rate of AIDS. However, there was a lack of evidence showing the optimal range of initial body mass index (BMI) patients maintain during antiretroviral therapy (ART). We aimed to evaluate associations of the BMI values pre-ART and during the entire ART duration with mortality among HIV-positive individuals. In total, 5101 HIV/AIDS patients, including 1439 (28.2%) underweight, 3047 (59.7%) normal-weight, 548 (10.7%) overweight and 67 (1.3%) obese patients, were included in this cohort. The cumulative mortality of underweight, normal-weight, and overweight were 2.4/100 person-years (95% CI 1.9–2.9), 1.1/100 person-years (95% CI 0.9–1.3), and 0.5/100 person-years (95% CI 0.1–0.9), respectively. Cumulative mortality was lower in both the normal-weight and overweight populations than in the underweight population, with an adjusted hazard ratio (AHR) of 0.5 (95% CI 0.4–0.7, p
- Published
- 2019
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30. Sphingosine kinase 1-interacting protein is a novel regulator of glucose-stimulated insulin secretion
- Author
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Yu Wang, Shin-ichi Harashima, Yanyan Liu, Ryota Usui, and Nobuya Inagaki
- Subjects
Medicine ,Science - Abstract
Abstract Glucose-stimulated insulin secretion (GSIS) is essential in keeping blood glucose levels within normal range. GSIS is impaired in type 2 diabetes, and its recovery is crucial in treatment of the disease. We find here that sphingosine kinase 1-interacting protein (SKIP, also called Sphkap) is highly expressed in pancreatic β-cells but not in α-cells. Intraperitoneal glucose tolerance test showed that plasma glucose levels were decreased and insulin levels were increased in SKIP−/− mice compared to SKIP+/+ mice, but exendin-4-enhanced insulin secretion was masked. GSIS was amplified more in SKIP−/− but exendin-4-enhanced insulin secretion was masked compared to that in SKIP+/+ islets. The ATP and cAMP content were similarly increased in SKIP+/+ and SKIP−/− islets; depolarization-evoked, PKA and cAMP-mediated insulin secretion were not affected. Inhibition of PDE activity equally augmented GSIS in SKIP+/+ and SKIP−/− islets. These results indicate that SKIP modulates GSIS by a pathway distinct from that of cAMP-, PDE- and sphingosine kinase-dependent pathways.
- Published
- 2017
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31. Publisher Correction: Emergence of Different Recombinant Porcine Reproductive and Respiratory Syndrome Viruses, China
- Author
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Yanyan Liu, Jianda Li, Jie Yang, Hao Zeng, Lihui Guo, Sufang Ren, Wenbo Sun, Zhi Chen, Xiaoyan Cong, Jianli Shi, Lei Chen, Yijun Du, Jun Li, Jinbao Wang, Jiaqiang Wu, and Jiang Yu
- Subjects
Medicine ,Science - Abstract
A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper.
- Published
- 2018
- Full Text
- View/download PDF
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