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Objective: To screen the differentially expressed genes (DEGs) in lesion tissues of children with congenital pulmonary airway malformation (CPAM) and to analyze their biological functions. Methods The pathological tissues and the adjacent tissues were collected from 5 children with CPAM through the thoracoscopic surgery. The RNA-seq libraries were established by Total RNA-seq (H/M/R) Library Prep Kit for Illumina® separately and we obtained their gene expression sequences by RSEM software, which were normalized by TMM method to obtain the effective sequences. After removing abnormal samples through principal component analysis, DEGs of CPAM were screened out by the edgeR software, and then GO function enrichment analysis and KEGG signal pathway analysis were performed by using cluster Profiler of the R software. Results Totally 1 063 DEGs (860 up-regulated genes and 203 down-regulated genes) were screened out after we eliminated 1 sample from 10 RNA-Seq libraries for abnormal expression. GO function enrichment analysis results showed that the cellular component was mainly located in axoneme, motile cilium, microtubule associated complex, apical part of cell, etc., the molecular function mainly included tubulin binding, ATPase activity, motor activity, microtubule binding, microtubule motor activity, etc., and the biological process mainly involved cilium organization, cilium assembly, microtubule-based movement, cilium movement, cilium and flagellum-dependent cell motility, etc. KEGG signaling pathway analysis results showed that DEGs were involved in the regulation of PI3K-Akt signaling pathway, vascular smooth muscle contraction, fluid shear stress and atherosclerosis, etc. Conclusion: Compared with CPAM adjacent tissues, there are 1 063 DEGs in CPAM lesion tissues, which are related to the formation and degeneration of the cilia, and participate in multiple signaling pathways related to the lung development. [ABSTRACT FROM AUTHOR]

全膝关节置换术 (TKA) 常导致剧烈的术后疼痛, 并影响患者术后早期活动及康复训练。以区域神经阻滞 为核心的围术期多模式镇痛是TKA术后镇痛的主要方式, 其中股神经阻滞曾是TKA术后镇痛金标准, 但其对股四 头肌肌力的影响限制了术后早期康复锻炼。近年来, 收肌管阻滞 (ACB) 因其镇痛效果好、对股四头肌肌力影响小等 特点, 逐渐被推荐用于TKA患者的术后镇痛。ACB的靶点神经为隐神经和股内侧肌神经 (NVM), 但NVM是否走行 于收肌管内仍有争议。ACB应用于 TKA术后镇痛中的最佳阻滞位点、局麻药容量及阻滞方式等相关研究较多, 但 目前尚无定论。ACB与关节周围局部浸润镇痛、ACB与腘动脉与后关节囊之间注射、ACB与局麻药佐剂等的联合 应用具有良好的镇痛效果, 是临床上常用的TKA术后镇痛策略。 [ABSTRACT FROM AUTHOR]

Objective To investigate the effect and molecular mechanism of ganglioside（GM-1）pretreatment on bupivacaine-induced pyroptosis of N2a cells. Methods N2a cells in the logarithmic phase were randomly divided into three groups： the bupivacaine group（B group，N2a cells were cultured with a final concentration of 900 μmol/L bupivacaine for 24 h），GM-1 pretreatment group（G+B group， after pretreatment with 5 μmol/L GM-1 for 24 h，N2a cells were cultured with 900 μmol/L bupivacaine for 24 h），and control group（C group，N2a cells were treated without GM-1 or bu⁃ pivacaine）. The cell morphology of each group was observed by the microscope and scanning electron microscope. Cell via⁃ bility was measured by CCK-8. TUNEL staining test was used to measure the TUNEL staining positive cell rate， and the rate of TUNEL stained positive cells indicated the rate of apoptosis. The lactate dehydrogenase（LDH）content was measured by LDH release experiment； the Caspase-1 activity experiment was used to calculate the Caspase-1 activity. Western blotting was used to detect the protein expression levels of inflammasome activating protein-gasdermin D（GSDMD），Nodlike receptor pyrin containing 3（NLRP3）and Caspase-1. Results The cell bodies of the C group were diverse and full with regular morphology, and the cell membrane was intact and smooth； cells of the B group shrunk and became round， and cell synapses were broken and disappeared， and apoptotic body-like cell protruding vesicles were produced； the cellular damage in morphology was significantly reduced， and there was no loss of cell membrane integrity and micropore formation in the G+B group. Compared with the C group， the viability of B group decreased， apoptosis rate and LDH content in⁃ creased and the relative expression of GSDMD，NLRP3，and Caspase-1 protein significantly increased （all P<0. 05）. Compared with B group， the cell viability of G+B group increased， apoptosis rate，LDH content and Caspase-1 activity decreased， and the relative expression levels of GSDMD，NLRP3，and Caspase-1 protein decreased（all P<0. 05）. Conclusions Bupivacaine can increase N2a cell membrane permeability， decrease cell viability and LDH release ，increase Cas⁃ pase-1 activity and apoptosis rate，and promote pyroptosis； the mechanism may be related to the increase in the relative protein expression of GSDMD，NLRP3，and Caspase-1. GM-1 pretreatment can inhibit the process of bupivacaine-in⁃ duced pyroptosis of N2a cells，and the mechanism may be related to the decrease of the relative protein expression of GSD⁃ MD，NLRP3，and Caspase-1. [ABSTRACT FROM AUTHOR]

细菌性阴道病是一种阴道微生物群系疾病，严重影响女性健康，尤其容易造成妊娠期不良妊娠结局，危害 母儿健康。阴道加德纳菌是细菌性阴道病的重要致病菌之一。阴道加德纳菌可黏附于阴道上皮，促进多微生物生 物膜的形成，促进多种促炎细胞因子、唾液酸酶及阴道溶细胞素等毒力因子的释放，从而影响机体的免疫反应；通过 释放某些信号来改变相关基因表达，同时与阴道微生态中某些微生物相互作用来改变阴道微生物群落组成，导致各 种不良结果。由于妊娠期的特殊性，关于阴道加德纳菌在妊娠期致病作用的研究相对较少，加德纳菌在形成生物 膜、释放毒力因子、促进其他微生物定植等方面的致病性与不良妊娠结局之间是否存在直接联系，目前仍未明确。 [ABSTRACT FROM AUTHOR]

Objective To observe the changes in levels of tumor necrosis factor-associated apoptosis-inducing ligand （TRAIL） in serum， and mitochondrial serine protease （OMI/HtrA2）in serum and skeletal muscle homogenate supernatant after intraperitoneal hydrogen injection in rabbits with limb ischemia-reperfusion （LIR） injury， and to explore their possible mechanisms. Methods Forty-five New Zealand big-ear white rabbits were randomly divided into the LIR + hydrogen group （group H），LIR group （group I），and control group （group C），with 15 rabbits in each group. In the groups H and I， the cuff was tied to the pulsating place of the femoral artery at the base of the right posterior limb of rabbits （blood flow could be completely blocked when the systolic blood pressure was 20-30 mmHg），and the balloon switch was opened when the cuff was tied for 4 h （reperfusion）. Rabbits in the group H were intraperitoneally injected with 5 mL/kg hydrogen 5 min before reperfusion， while group C and I with the same volume of air at the same time， and in the group C， we only used the same cuff to tie the same part of rabbits. Venous blood and tibialis anterior muscle tissues of big-ear white rabbits were collected at 24，72 and 168 h after reperfusion， respectively， and we detected TRAIL， Omi/HtrA2 in serum and Omi/HtrA2 in skeletal muscle tissues. Results Compared with the group I， the levels of TRAIL and Omi/HtrA2 decreased in the serum of group H at 24，72 and 168 h after reperfusion （all P<0. 01）；compared with the group I， the level of Omi/HtrA2 in the homogenate supernatant of skeletal muscle tissues decreased in the group H at 24，72 and 168 h after reperfusion （all P<0. 01）. Compared with the same group at different time periods， there were statistically significant differences in serum TRAIL and skeletal muscle tissue homogenate supernate Omi/HtrA2 between the groups I and H （all P< 0. 01），and there was statistically significant difference in the serum Omi/HtrA2 between the rabbits in the group I（P< 0. 01）. Conclusions The levels of TRAIL and Omi/HtrA2，and Omi/HtrA2 expression in the skeletal muscle homogenate supernatant decrease after intraperitoneal injection of hydrogen during LIR injury in rabbits. TRAIL and Omi/HtrA2 may be involved in the development and progression of LIR. Hydrogen can inhibit apoptosis by inhibiting the activation of TRAIL and the release of Omi/HtrA2. [ABSTRACT FROM AUTHOR]

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

动脉粥样硬化(AS)是心脑血管疾病中致死致残的主要病因之一,其危险因素主要有高血压、糖尿病、脂质代谢紊乱、吸烟、肥胖等,在这些危险因素的作用下动脉血管壁受到损伤,脂质沉积于管壁而引起管壁变硬,管腔狭窄形成AS,引起心脑血管疾病。目前对于AS的研究更多倾向于表观遗传学方面,是指在不改变DNA序列的前提下对DNA碱基和组蛋白进行可遗传性共价修饰,其中基因组DNA甲基化改变是目前研究的热点。同型半胱氨酸、雌激素受体基因、单羧酸转运蛋白、组织因子途径抑制物2、细胞外超氧化物歧化酶、p53基因、TET2基因的DNA甲基化会加剧AS,而AS危险因素如脂代谢紊乱、原发性高血压、糖尿病、高龄等均与DNA甲基化有关。 [ABSTRACT FROM AUTHOR]

目的 探讨血清抗心磷脂抗体 (ACA 汃可溶性血栓调节蛋白 (sTM) 及和肤索 (Copeptin) 水平对脑梗死患 者神经功能缺损程度的评估价值。方法 选取脑梗死患者60例（梗死组, 根据神经功能缺损评分(NDS)15]将患 者分为中型(NDS评分 16 ~30分）23 例 、重型 (NDS 评分＞31分）37 例。采用酶联免疫法检浏血清 ACA、Copeptin, 采用双抗体夹心法检测血清 sTM；采用 Spearman 相关分析法分析血清 ACA、sTM及 Copeptin 水平与 NDS 评分的相 关性；采用受试者工作特征 (ROC) 曲线分析 ACA、sTM 及 Copeptin 对脑梗死患者神经功能缺损程度的评估价值。 结果 梗死组血清 ACA、sTM 及Copeptin水平商于对照组 (P 均<0.05)，重型组血清 ACA、sTM 及 Copeptin 水平商 于中型组 (P均<0.05)。 血清 ACA、sTM、Copeptin 水平与 NDS 评分呈正相关(r分别为 0.776、0.876、0.634,P均< 0.05)。中型组血清 ACA、sTM、Copeptin 及三者联合检涓评估脑梗死患者神经功能缺损程度的曲线下面积分别为 0.512、0.603、0.692、0.775，重型组分别为 0.654、0.792、0.778、0.832。结论 血清 Copeptin、sTM、ACA 水平可评估 脑梗死患者神经功能缺损程度。 [ABSTRACT FROM AUTHOR]

胸椎旁神经阻滞应用于胸科手术能加强术中术后的镇痛效果,改善患者术后肺功能,利于患者康复,相比于胸椎硬膜外、肋间神经阻滞等还有其独特的优势。进行椎旁神经阻滞时有单点、两点、多点穿刺等多种选择方式。为加强胸椎旁神经阻滞的镇痛效果、延长镇痛时间,并减少局麻药及术后补救镇痛药物的使用剂量,可选择芬太尼、可乐定、右美托咪定及硫酸镁等作为辅助用药。利用超声引导行胸椎旁神经阻滞可减少其并发症的发生率,进一步提高操作的安全性。 [ABSTRACT FROM AUTHOR]

Objective To investigate the inhibitory effects of polysaccharide from sugarcane leaves on the cardiomyo-cyte apoptosis in rats withmyocardial infarction(MI). Methods We established the MI models by ligating the left anterior descending (LAD) of rats, and then 30 rats were randomly divided into themodel control group (MI group) , sugarcane leaf polysaccharide group (SLP group) , in addition, sham operation group was set, however, the rats in the sham group were only threaded without ligation. The rats in the SLP group were fed with 4 mg/mL SLP solution, and the rats in the MI group and sham group were fed with the same volume of normal saline. After 4 weeks, the rats heart was obtained by thoracotomy , we measured the myocardial apoptosis in the infarct marginal zone by TUNEL and performed HE staining, then we detected the gene expression of BCL-2, BAX, and Caspase3 by real-time PGR, detected the protein expression of p-AKT/ AKT and p-PI3K /PI3K at the edge of myocardial infraction by Western blotting. Results The apoptosis in rats of the sham group was not detected, but was detected in the marginal zone of the MI group and SLP group. The apoptotic index of the sham group, MI group, and SLP group were 0.017% ±0.007% , 0.307% ±0.048% , and 0.109% ±0.034% , respectively. There was significant difference in the apoptotic index of myocardial cells between every two groups (P < 0.05) . Compared with the sham group, myocardial apoptosis index increased in the MI group and SLP group, but myocardial apoptosis index in the SLP group was lower than that in the MI group (all P < 0.05). Compared with the sham group, BAX, BCL-2, and Caspase-3 increased in the MI group (P <0.05) . Compared with the MI group, the expression of BCL-2 mRNA increased, while the expression of BAX and Caspase-3 mRNA decreased in the SLP group (all P <0.05) . Compared with the sham group, the protein expression of p-AKT/AKT and p-PI3K/PI3K decreased in the MI group (P < 0.05) . Compared with the MI group, the expression of p-AKT/AKT and p-PI3K/PI3K increased in the SLP group(both P <0.05). Conclusions SLP could significantly inhibit the apoptosis of myocardial cells after MI,and its mechanism may be that SLP may increase the expression of the anti-apoptosis gene BCL-2, decrease the expression of pro-apoptosis gene BAX, Caspase-3, and up-regulate the expression of p-AKT/AKT and p-PI3K/PI3K protein. [ABSTRACT FROM AUTHOR]

蛋白酶激活受体2(PAR2)是G蛋白偶联受体家族成员,由细胞内袢与C-末端、7个跨膜螺旋结构、N-末端与细胞外袢组成,广泛分布于各个组织系统,包括表皮、心血管、免疫系统、外周与中枢神经系统等,通过跨膜信号转导调节细胞的各种功能。骨癌疼痛大多数是由癌症转移到骨引起,常常伴随骨相关事件发生,严重影响患者生存质量。活化的PAR2通过调节相关的特殊信号通路,如P物质、降钙素基因相关肽、脑源性神经营养因子、瞬时感受器电位通道、丝裂原激活的蛋白激酶/细胞外信号调节激酶、N-甲基-D-天门冬氨酸受体、蛋白激酶A、蛋白激酶C、µ阿片受体等,参与骨癌疼痛痛觉敏化及痛觉信息的传递和调控。 [ABSTRACT FROM AUTHOR]

青蒿素是具有过氧基团的倍半萜内酯类化合物,其衍生物主要包括双氢青蒿素、青蒿琥酯、蒿甲醚和蒿乙醚等。青蒿素及其衍生物对肝癌、肺癌、胃癌、结直肠癌、卵巢癌等多种肿瘤细胞具有增殖抑制或杀伤作用,且具有安全、高效、不良反应少、对多药耐药细胞有效等优点。 [ABSTRACT FROM AUTHOR]

目的缺血性心力衰竭是由于冠状动脉狭窄或闭塞引起心肌缺血、心脏收缩和(或)舒张功能受损、出现体循环或肺循环淤血、组织血流灌住不足、活动耐力下降并伴或不伴心绞痛的一组临床综合征。 其非药物治疗包括冠脉血运重建, 缺血性心衰演变为顽固性心衰借助心脏再同步治疗或心脏收缩调节治疗改善心脏泵血, 终末期心衰行心脏移植替代治疗。 此外, 将自体分化成熟的干细胞整合到心脏组织、调节心肌细胞周期及腺病毒相关病毒9介导的miR-199a和miR-590过表达促进心肌梗死后心肌细胞的增殖与再生、利用纳米颗粒将生物分子递送到冠状动脉粥样硬化部位等非药物治疗方式有望成为缺血性心衰治疗的新策略。 [ABSTRACT FROM AUTHOR]

右美托咪定（Dex)是高选择性α2肾上腺素能受体f动剂，除具有镇静镇痛、无呼吸抑制等特点外，还.降低术后躁动、寒战等的发生率，与局麻药混合后行神经阻滞^能增加镇痛效果、延长术后镇痛时间，故在小儿.术中的运用也逐渐广泛。Dex作为小儿神经阻滞术前用药具有镇静、抗焦虑、无呼吸抑制的优点，给药途径可选.经鼻途径;Dex可作为神经阻滞术中清醒镇静的维持药物，在镇静的同时无静脉刺激、无呼吸抑制、对循环波动影.也较小;Dex加入局麻药行神经阻滞在手术过程中可缩短局麻药物的起效时间，增加感觉神经的阻滞效果，延长.动神经的阻滞时间;Dex加人局麻药行神经阻滞在手术后可增加局麻药的镇痛效果，减少患儿术后阿片类等补救.痛药物的用量。 [ABSTRACT FROM AUTHOR]

目的 探讨短期低剂量甲状腺激素替代疗法对慢性阻塞性肺疾病(COPD)合并非甲状腺疾病综合征患者的疗效。方法 选取COPD合并非甲状腺疾病综合征患者146例,随机分为对照组、短期治疗组和长期治疗组,对照组只进行对症治疗,短期和长期治疗组在对症治疗的基础上、TSH分别进行为期1周和1个月的低剂量甲状腺激素替代治疗,并于治疗前、治疗结束后对患者的肺功能[第1秒用力呼气容积(FEV1)和第1秒用力呼气容积占用力肺活量百分比(FEV_1/FVC)]、甲状腺激素水平(包括总T3、总T4、游离T3、游离T4、TSH)、细胞因子水平(包括TNF-α、IL-6、IL-10、TGF-β)以及淋巴细胞亚群(包括CD3+T、CD4+T、CD8+T和CD4+CD25+T细胞)进行测定。结果 与治疗前比较,治疗后对照组FEV_1%pred及FEV1/FVC升高,短期及长期治疗组FEV1%pred、FEV1/FVC、FT3、TT3、TT4、IL-10升高,FT4、IL-6、TGF-α、TGF-β降低(P均<0.05)。与对照组比较,短期治疗组FEV_1%pred、FEV_1/FVC、FT3、FT4、TT3、TT4升高;长期治疗组FEV1%pred、FEV1/FVC、FT3、FT4、TT3、TT4升高,TSH、TGF-β降低(P均<0.05);两治疗组CD4+、CD25+T细胞百分比升高、CD4+T细胞百分比降低(P均<0.05)。两治疗组间除TSH水平差异有统计学意义外,其他指标比较,P均<0.05。结论 短期低剂量甲状腺激素替代疗法治疗COPD可以改善患者呼吸功能,降低血清炎性因子以及改善外周血T淋巴细胞亚群的分布,与长期低剂量甲状腺激素替代治疗效果相当,但不良作用少。 [ABSTRACT FROM AUTHOR]

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

目的分析甲状腺功能亢进症(简称甲亢)患者并发肺动脉高压的危险因素。方法选取126例甲亢患者,其中单纯甲亢患者86例、甲亢合并肺动脉高压患者40例,采用χ2检验进行单因素分析比较两者性别、年龄、甲亢病程、BMI、吸烟史、饮酒史、左心室射血分数(LVEF)、心率、血清总三碘甲状腺原氨酸(TT3)、血清总甲状腺素(TT4)、甲状腺激素(TSH),采用logistic回归对甲亢并发肺动脉高压危险因素进行多因素分析。结果单因素分析结果显示,单纯甲亢患者与甲亢合并肺动脉高压患者性别、年龄、饮酒、LVEF、TT3比较P均<0.05。多因素logistic回归分析显示,甲亢患者并发肺动脉高压的相关因素有年龄(OR=1.068,95%CI:0.862~0.974,P<0.01)、饮酒(OR=0.721,95%CI:1.189~2.135,P<0.05)、LVEF(OR=0.911,95%CI:1.035~1.672,P<0.05)。结论高龄、饮酒、左心室功能减弱是甲亢并发肺动脉高压的危险因素。 [ABSTRACT FROM AUTHOR]

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

目的 观察改良腹腔镜联合胆道镜手术治疗肝外胆道系统结石的临床效果。方法选取肝外胆道系统结石患者43例，随机分为传统组25例、改良组18例。传统组采用传统四孔法建立腹腔镜手术路径，改良组采用改良五孔法建立腹腔镜手术路径，均先行腹腔镜胆囊切除术，然后经剑突下10 mm置入胆道镜，进行胆总管取石。统计两组术中转开腹例数、胆管缝合时间、手术时间、术中出血量等指标；采用疼痛程度数字评分法（NRS）评价术后24 h疼痛程度；术后随访12个月，统计胆瘘、胆管结石残留、切口疝、粘连性肠梗阻、胆管狭窄等并发症发生情况。结果传统组22例（88．00％）、改良组16例（88．89％）成功实施手术，两组比较P＞0．05。传统组胆管缝合时间、手术时间均长于改良组（P均＜0．05），两组术中转开腹例数、术中出血量、术后24 h NRS评分、术后自主下床活动时间、通气时间、引流管留置时间、住院时间比较差异均无统计学意义（P均＞0．05）。术后随访12个月，两组均未出现切口疝、粘连性肠梗阻、胆管狭窄等并发症；传统组出现胆瘘3例、胆管结石残留1例，改良组分别为1、2例，两组比较P均＞0．05。结论改良腹腔镜联合胆道镜手术治疗肝外胆道系统结石可缩短胆管缝合时间、手术时间，且较为安全。 [ABSTRACT FROM AUTHOR]

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

目的：总结腹腔妊娠的诊治经验。方法回顾性分析1例经剖腹取胎术治疗的腹腔妊娠患者临床资料，并复习相关文献。结果患者停经后超声提示宫内早孕，行清宫术＋宫内节育器放置术后出现不规则阴道流血、发热伴下腹痛。查血人绒毛膜促性腺激素（HCG）97871 mIU／mL，超声提示腹腔内妊娠囊，确诊为腹腔妊娠。行剖腹取胎术，刺破妊娠囊取出胎儿后，胎盘附着处大量出血，腹腔留置纱布压迫止血。3天后，行二次手术取出腹腔纱布，术中见胎盘附着于结肠系膜，血运丰富，故未处理胎盘。术后患者血HCG进行性下降，3个月后出现下腹部疼痛，给予第3次剖腹手术，取出胎盘。术后血HCG 0．12 mIU／mL，患者恢复正常。结论腹腔妊娠无特异性临床表现，需结合实验室检查进行确诊，临床误诊率较高；其治疗以剖腹取胎术为主，术中根据胎盘附着部位给予针对性处理。 [ABSTRACT FROM AUTHOR]

目的：探讨嗜麦芽窄食单胞菌临床分布及耐药性，为临床合理用药提供依据。方法收集2013年1月～2014年12月本溪市中心医院常规培养检出的嗜麦芽窄食单胞菌菌株，分析检出菌株的标本分布、病房分布及其耐药情况。结果本研究共检出嗜麦芽窄食单胞菌77株，检出菌株的标本分布以痰液为主（占72．7％），病房分布以干部病房和ICU病房为主（分别占35．1％和15．6％）。药物敏感试验结果显示，检出的嗜麦芽窄食单胞菌对四环素耐药率最高（100％），其次为头孢他啶（77．6％），对左氧氟沙星耐药率较低（10．4％）。结论嗜麦芽窄食单胞菌主要从痰液中检出，对四环素、头孢他啶耐药严重。 [ABSTRACT FROM AUTHOR]