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1. AbSeq Protocol Using the Nano-Well Cartridge-Based Rhapsody Platform to Generate Protein and Transcript Expression Data on the Single-Cell Level

Author

Jami R. Erickson, Florian Mair, Grace Bugos, Jody Martin, Aaron J. Tyznik, Margaret Nakamoto, Stefanie Mortimer, and Martin Prlic

Subjects
Science (General), Q1-390
Abstract

Summary: By including oligonucleotide-labeled antibodies into high-throughput single-cell RNA-sequencing protocols, combined transcript and protein expression data can be acquired on the single-cell level. Here, we describe a protocol for the combined analysis of over 40 proteins and 400 genes on over 104 cells using the nano-well based Rhapsody platform. We also include a workflow for sample multiplexing, which uniquely identifies the initial source of cells (such as tissue type or donor) in the downstream analysis after upstream pooling.For complete information on the use and execution of this protocol, please refer to Mair et al. (2020).

Published
2020
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2. AbSeq Protocol Using the Nano-Well Cartridge-Based Rhapsody Platform to Generate Protein and Transcript Expression Data on the Single-Cell Level

Author

Stefanie Mortimer, Margaret Nakamoto, Aaron J. Tyznik, Jody L. Martin, Grace Bugos, Florian Mair, Martin Prlic, and Jami R. Erickson

Subjects
Proteomics, General Immunology and Microbiology, Sequence Analysis, RNA, Computer science, Gene Expression Profiling, General Neuroscience, Gene Expression, High-Throughput Nucleotide Sequencing, Proteins, Computational biology, Cellular level, Multiplexing, Article, Antibodies, General Biochemistry, Genetics and Molecular Biology, Workflow, Tissue type, Upstream (networking), Single-Cell Analysis, Transcriptome, lcsh:Science (General), Protocol (object-oriented programming), Gene, Gene transcript, lcsh:Q1-390
Abstract

Summary By including oligonucleotide-labeled antibodies into high-throughput single-cell RNA-sequencing protocols, combined transcript and protein expression data can be acquired on the single-cell level. Here, we describe a protocol for the combined analysis of over 40 proteins and 400 genes on over 104 cells using the nano-well based Rhapsody platform. We also include a workflow for sample multiplexing, which uniquely identifies the initial source of cells (such as tissue type or donor) in the downstream analysis after upstream pooling. For complete information on the use and execution of this protocol, please refer to Mair et al. (2020) .

Published
2020

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