1. Effects of combinations of Fusarium mycotoxins on the inhibition of macromolecular synthesis, malondialdehyde levels, DNA methylation and fragmentation, and viability in Caco-2 cells
- Author
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Sébastien D. Dano, Théophile A. Mobio, James H. Kouadio, Edmond E. Creppy, Serge Moukha, Université de Bordeaux Ségalen [Bordeaux 2], Université Félix Houphouët-Boigny (UFHB), Unité de recherche Mycologie et Sécurité des Aliments (MycSA), and Institut National de la Recherche Agronomique (INRA)
- Subjects
DNA damage ,Cell Survival ,Apoptosis ,DNA Fragmentation ,Biology ,CELLULAR MACROMOLECULES SYNTHESIS ,Toxicology ,Fumonisins ,03 medical and health sciences ,chemistry.chemical_compound ,0404 agricultural biotechnology ,Fusarium ,Malondialdehyde ,DEUTEROMYCOTINA ,Humans ,Drug Interactions ,Viability assay ,Gene Silencing ,Fragmentation (cell biology) ,SYNTHESE ,Zearalenone ,030304 developmental biology ,0303 health sciences ,Fumonisin B1 ,DNA synthesis ,FUSARIUM TOXIN ,food and beverages ,04 agricultural and veterinary sciences ,DNA Methylation ,Mycotoxins ,CELL VIABILITY ,040401 food science ,LIGNEE CACO-2 ,Drug Combinations ,Oxidative Stress ,Enterocytes ,Biochemistry ,chemistry ,[SDV.TOX]Life Sciences [q-bio]/Toxicology ,INTERACTIVE EFFECT ,DNA fragmentation ,HUMAN INTESTINAL CACO-2 CELL ,Lipid Peroxidation ,Caco-2 Cells ,Trichothecenes ,DNA - Abstract
International audience; We studied the interactive effects of either binary or tertiary mixtures of Fusarium mycotoxins, deoxynivalenol (DON), zearalenone (ZEA), and fumonisin B1 (FB1) on the human intestinal cell line, Caco-2, using the endpoints including malonedialdehyde (MDA) production, inhibition of protein and DNA syntheses, DNA methylation, DNA fragmentation, and cell viability as measured by the neutral red (NR) test. The mixtures of mycotoxins reduce cellular viability in increasing order: [FB1+ZEA]
- Published
- 2006
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