Search

Your search keyword '"Fachado A"' showing total 5 results
Start Over Author "Fachado A" Journal vaccine
5 results on '"Fachado A"'

2. Protective effect of a naked DNA vaccine cocktail against lethal toxoplasmosis in mice

Author

Regina R. Amendoeira, Diamar C. Pinto, Armando Acosta, Alexandro Rodriguez, Alberto Fachado, Iviane Vila, Joseli Lannes-Vieira, and Sergio O. Angel

Subjects
Male, Protozoan Vaccines, animal diseases, DNA vaccination, Interferon-gamma, Mice, chemistry.chemical_compound, Plasmid, parasitic diseases, Vaccines, DNA, Animals, Gene, Mice, Inbred BALB C, General Veterinary, General Immunology and Microbiology, biology, Vaccination, Public Health, Environmental and Occupational Health, Toxoplasma gondii, Th1 Cells, biology.organism_classification, Virology, Toxoplasmosis, Animal, Infectious Diseases, chemistry, Immunization, Naked DNA, Molecular Medicine, Toxoplasma, DNA
Abstract

BALB/c mice were intramuscularly immunized with low doses (25-50microg) of DNA cocktail containing plasmids encoding the full-length SAG1/P30 and the 196-561 terminal sequence of ROP2 genes. This immunization resulted in a Th1-type response with predominance of IgG2a and a specific T-cell proliferation with high levels of interferon-gamma (IFN-gamma) secretion, whereas no IL-4 was detected. Moreover, DNA cocktail immunization induced a long-lasting protection against a lethal challenge with the highly virulent Toxoplasma gondii RH strain, whereas low doses of single genes were not protective. These results support further investigations to achieve a multigene anti-T. gondii DNA vaccine.

Published
2003
Full Text
View/download PDF

3. Specific cellular and humoral immune response in Balbc mice immunised with an expression genomic library of Trypanosoma cruzi

Author

J. F. Infante, Luis Izquierdo, Alberto Fachado, Maria E. Sarmiento, Luis Fonte, Armando Acosta, G. Sierra, Esteban Alberti, Carlos M. Finlay, Teresita Vidal, and Carlos Hidalgo

Subjects
Protozoan Vaccines, Cellular immunity, Trypanosoma cruzi, Enzyme-Linked Immunosorbent Assay, Lymphocyte Activation, Mice, Immune system, Antigen, Antibody Specificity, parasitic diseases, Vaccines, DNA, Animals, Immunity, Cellular, Mice, Inbred BALB C, General Veterinary, General Immunology and Microbiology, biology, Immunogenicity, Public Health, Environmental and Occupational Health, DNA, Protozoan, biology.organism_classification, Virology, Infectious Diseases, Immunoglobulin G, Antibody Formation, Humoral immunity, biology.protein, Molecular Medicine, Female, Antibody, Genome, Protozoan, Lymphoproliferative response
Abstract

An expression genomic library of Trypanosoma cruzi (T. cruzi) constructed using pcDNA3 plasmid was used for the immunisation (25 ug) of Balblc mice. Expression of T. cruzi antigens in the muscle of inoculated mice was detected by indirect immunofluorescence 7 days after immunisation. Specific IgG antibodies were significatively increased (P ~0.05) in animals that were reimmunised with 50 ,ug of the genomic library. An antigen specific lymphoproliferative response was detected in one animal of 0 1998 Elsevier Science Ltd. All the group inoculated with one dose of the library rights reserved

Published
1998
Full Text
View/download PDF

4. Specific cellular and humoral immune response in Balbc mice immunised with an expression genomic library of Trypanosoma cruzi

Author

Alberti, Esteban, primary, Acosta, Armando, additional, Sarmiento, Maria E., additional, Hidalgo, Carlos, additional, Vidal, Teresita, additional, Fachado, Alberto, additional, Fonte, Luis, additional, Izquierdo, Luis, additional, Infante, Juan F., additional, Finlay, Carlos M., additional, and Sierra, Gustavo, additional

Published
1998
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results