1. Higher catalytic efficiency of N-7-methylation is responsible for processive N-7 and 2'-O methyltransferase activity in dengue virus.
- Author
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Chung KY, Dong H, Chao AT, Shi PY, Lescar J, and Lim SP
- Subjects
- Adenosine analogs & derivatives, Adenosine pharmacology, Animals, Enzyme Inhibitors pharmacology, Inhibitory Concentration 50, Kinetics, Methylation, Methyltransferases antagonists & inhibitors, S-Adenosylmethionine metabolism, Viral Proteins antagonists & inhibitors, Dengue Virus enzymology, Methyltransferases metabolism, RNA metabolism, Viral Proteins metabolism
- Abstract
Methyltransferases (MTases) from the genus Flavivirus encode both N-7 and 2'-O activities needed for type 1 (m(7)GpppNm) cap structure formation. We performed kinetic studies to understand the mechanisms of its progressive N-7 and 2'-O methylations. Sequential N-7 to 2'-O methylation occurred via a random bi bi and processive mechanism that does not involve enzyme-RNA dissociation. Analyses of steady state kinetic parameters showed that N-7 precedes 2'-O methylation as it turnovers RNA faster (k(cat)) resulting in 2.4-fold higher catalytic efficiency. Michaelis constants for S-adenosyl-methionine (AdoMet) in both reactions were about 10-fold lower than for their respective RNA substrates, suggesting that the rate-limiting steps in methylase reactions were associated with RNA templates. In the context of long viral RNA sequences, and compared to S-adenosyl-homocysteine, sinefungin was about 60- and 12-folds more potent against dengue N-7 and 2'-O MTase activity, exhibiting IC(50) values of 30 and 41nM, respectively., (Copyright (c) 2010 Elsevier Inc. All rights reserved.)
- Published
- 2010
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