1. Effect of parathyroid hormone on hypogonadism induced bone loss of proximal femur of orchiectomized rat.
- Author
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Tezval M, Serferaz G, Rack T, Kolios L, Sehmisch S, Schmelz U, Tezval H, Stuermer KM, and Stuermer EK
- Subjects
- Animals, Biomechanical Phenomena drug effects, Biomechanical Phenomena physiology, Body Weight drug effects, Body Weight physiology, Bone Density drug effects, Bone Density physiology, Disease Models, Animal, Dose-Response Relationship, Drug, Femur drug effects, Femur pathology, Injections, Subcutaneous, Male, Osteoporosis physiopathology, Parathyroid Hormone administration & dosage, Parathyroid Hormone pharmacology, Rats, Rats, Sprague-Dawley, Femur physiopathology, Hypogonadism complications, Orchiectomy, Osteoporosis drug therapy, Osteoporosis etiology, Parathyroid Hormone therapeutic use
- Abstract
Purpose: Management of hypogonadism-induced osteoporosis in elderly men is still a challenge. We investigated the short-term effects of parathyroid hormone (PTH) treatments on strength, micro-architecture, and mineral density of trochanteric region of orchiectomized rat femur., Methods: Eight-month-old male Sprague-Dawley rats (n = 44) were divided into two groups: (1) orchiectomized (ORX) and (2) sham group. Twelve weeks after orchiectomy, half of the orchiectomized animals were treated with daily subcutaneously injected PTH (0.040 mg/kg/BW) (ORX-PTH) for 5 weeks. The other half remained untreated (ORX). The sham-operated group was divided and treated in the same way (sham, sham-PTH). After 5 weeks, both femurs were excised for biomechanical and histomorphometric analysis, trabecular measurements, mineral content assessment, and immunofluorescence analysis., Results: The femoral trochanteric strength after PTH treatment was enhanced in the breaking test (ORX-F(max) = 158.7 N vs. ORX + PTH-F(max) = 202 N). Stiffness of treated ORX animals reached nearly the levels observed in untreated sham rats. PTH therapy improved the trabecular connectivity, width, and area (ORX-Tb.Ar = 47.79% vs. ORX + PTH-Tb.Ar = 68.47%, P < 0.05) in the proximal femur. The treated rats showed significantly improved mineral content in ashed femurs (ORX-mineral content = 43.73% vs. ORX + PTH-mineral content = 49.49%) when compared to the untreated animals. A comparison of widths of fluorescence bands in cortical bone of the subtrochanteric cross-sections showed a significant increase in oppositions after the PTH therapy., Conclusions: Our finding supports the hypothesis that PTH therapy seems to be a rational therapy in patients with hypogonadism induced bone loss and improves the bone strength of trochanteric region of rat femur.
- Published
- 2011
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