Search

Objective To analyze the routine test parameter levels of patients with colorectal adenoma and colorectal cancer, and develop a prediction model. Methods A total of 580 patients diagnosed with colorectal adenoma (117 patients) and colorectal cancer (463 patients) were included in the retrospective study. The patients were randomly divided into two groups according to a 7:3 ratio: a training set with 406 cases and a validation set with 174 cases. Logistic regression analysis was used to establish a prediction model, and a nomogram was drawn. The model′s discrimination, calibration, and clinical applicability were evaluated using receiver operating characteristic curve (ROC), calibration plot, and decision curve analysis (DCA). Results Univariate logistic regression analysis identified 13 potential predictors: age, fecal occult blood test (FOBT), fibrinogen (FIB), thrombin time (TT), albumin (ALB), white blood cell value (WBC), neutrophil count (NEUT#), hematocrit value (HCT), mean corpuscular hemoglobin (MCH), red cell distribution width (RDW), platelet count (PLT), mean platelet volume (MPV), and activated partial thromboplastin time (APTT). Multivariate logistic regression analysis showed MPV, FIB, ALB, FOBT, TT, and HCT were risk factors for colorectal cancer in patients with colorectal adenoma (P

Objective To explore the advantages and disadvantages of S-poly(T) plus qRT-PCR and Poly (A) qRT-PCR methods in detecting peripheral blood serum miR-199. Methods S-poly(T) plus RT-PCR and Poly(A) qRT-PCR methods were used to detect the serum miR-199 in patients with liver cancer and healthy controls in three aspects of sensitivity, linearity and repeatability to evaluate their potential clinical value. Results The Ct values of S-poly(T) plus qRT-PCR method for miR-199 in healthy controls was significantly lower than that of Poly(A) qRT-PCR method (P < 0.0001); and the S-poly(T) plus qRT-PCR method had better linear correlation (P=0.0027) and repeatability (P=0.010). S-poly(T) plus qRT-PCR method had better sensitivity in detecting serum miR-199 in liver cancer patients comparing with Poly (A) qRT-PCR method. Conclusion In the detection of liver cancer serum miR-199, S-poly(T) plus qRT-PCR method is more sensitive, repeatable and stable than Poly(A) qRT-PCR.

Objective: To investigate the role of Th17/Treg unbalance in the pathogenesis of experimental autoimmune encephalomyelitis (EAE)., Methods: EAE was modeled in mice and the number of regulatory T cells (Tregs) in spleen of EAE mice was detected by flow cytometry. The expressions of Foxp3 and RoR-γt mRNA in the spleen of EAE mice and IL-17 mRNA in the brain of EAE mice were evaluated by real-time quantitative PCR and the levels of IL-6, TGF-β and IL-17 in the serum of EAE mice were examined by ELISA., Results: Compared with control group, the number of CD4(+)CD25(+) Foxp3(+) Tregs and the expression of Foxp3 mRNA in the spleen of EAE mice dramatically decreased in the early and peak stage of EAE (P<0.05), but increased in chronic stage of EAE (P<0.05); the RoR-γt mRNA expression from mouse spleen at the early stage of EAE was significant raised (P<0.05), but was not significantly different at the peak and chronic stage of EAE from that in control group (P>0.05). The levels of IL-6 and TGF-β in the serum of EAE group dramatically increased compared with control group (P<0.05). With the development of EAE, the level of IL-6 gradually decreased, and there was no statistical difference in the chronic stage of EAE compared with control group (P>0.05). However, the level of TGF-β was higher than that in control group in the chronic stage of EAE (P<0.05). Compared with those in control group, the concentration of IL-17A and the expression of IL-17 mRNA dramatically increased in different stages of EAE group, especially in peak stage (P<0.05)., Conclusion: Th17/Treg unbalance may be involved in the pathogenesis of EAE.