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Copyright of Food & Fermentation Industries is the property of Food & Fermentation Industries and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

【Objective】Rhizobium yanglingense CCBAU 01603 form efficient nitrogen-fixing nodules with Caragana legumes. Alk_40, one of the offspring of CCBAU 01603, was obtained through experimental evolution in alkaline medium for 500 generations. It was discovered that a significant decrease in exopolys accharide（EPS）yields of Alk_40 with specific SNPs changes in the gene envZ, iscS, and asnC. EPS yields and environmental adaptability of rhizobia regulated by these key genes were investigated.【Method】Three mutants of rhizobia were created by deleting gene envZ, iscS, and asnC, respectively. EPS yields and growth under different environmental conditions of mutants were assessed.【Result】In comparison to CCBAU 01603, the envZ, iscS, and asnC mutants presented significantly lower EPS yields in YMA medium, demonstrated diminished fitness in acidic environments, and had a weakened tolerance to high temperatures of 65℃ . This observation suggests a correlation between the EPS yield of rhizobia and their resistances to acidic or heat stress. In addition, the mutants lacking the envZ and asnC genes showed the sensitivity to NaCl, resulting in severely limited growth and reproduction in NaCl-containing media. Furthermore, their adaption in alkalescent environments significantly reduced as well.【Conclusion】It is discovered that EPS yields of rhizobia can be regulated by gene envZ, iscS, and asnC. [ABSTRACT FROM AUTHOR]

Copyright of China Brewing is the property of China Brewing Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Based on the bibliometrics of scientific literature, CiteSpace and VOSviewer software are used to investigate exopolysaccharides (EPS) in Web of Science (WOS) core collection database and China National Knowledge Infrastructure (CNKI) database from 2013 to 2023. In the field of EPS, 4047 English literatures and 1212 Chinese literatures are analyzed by quantitative statistics and visualization. The research status and hotspots of EPS are revealed by the perspectives of annual publication numbers, authors, highly cited literatures, publication journals, publication institutions, publication countries, keyword co-occurrences and emergences. The results shows that the overall number of published papers in the field of EPS is increasing year by year. Major contributors are Dertli Enes, De Philippis Roberto, Xu Chunping and so on, whereas the communication and cooperation among authors needto be strengthened. The most highly cited literatures in the WOS and CNKI databases are Strategies for combating bacterial biofilms: A focus on anti-biofilm agents and their mechanisms of action and Investigation of Biofilm formation Mechanism and its Influencing Factors, respectively. The top three journals are International Journal of Biological Macromolecules, Frontiers in Microbiology and Carbohydrate Polymers. The top three institutions are Centre National de la Recherche Scientifique (CNRS), Egyptian knowledge bank (EKB) and Chinese Academy of Sciences. The publication countries mainly are China, India and the United States, whereas academic impact of China in the field of EPS need to be improved. Keyword co-occurrence analysis shows that the hot spots are optimization of fermentation conditions, developement of EPS function and production EPS by lactic acid bacteria. It reveals that mining of biological function, biosynthesis and industrial application of EPS are the main directions of future research. Based on bibliometrics, this paper expounds and analyzes the research status and hot spots in the field of EPS, providing certain references for future research.

Copyright of Journal of Chinese Institute of Food Science & Technology / Zhongguo Shipin Xuebao is the property of Journal of Chinese Institute of Food Science & Technology Periodical Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The objective of this study was to screen lactic acid bacteria strains with high exopolysaccharides production potential from sugar beet molasses. The taxonomic identification and growth characteristics of a lactic acid bacteria strain M1 with high exopolysaccharides-producing capacity isolated from sugar beet molasses were investigated. Furthermore, the exopolysaccharides fermentation parameters using sugar beet molasses as an alternative carbon source to sucrose were optimized using a single-factor experiment and response surface methodology based on Box-Behnken design. Moreover, antioxidant activity of the harvested crude exopolysaccharides were evaluated. The results showed that strain M1 was taxonomically classified as Leuconostoc suionicum. The growth profile of L. suionicum M1 indicated a lag phase of 2 hours, an optimal temperature of 35 °C, a favorable pH value of 8. 0, and a remarkable tolerance to sucrose concentrations up to 60%. The optimal medium for exopolysaccharides production consisted of 300 g/L sugar beet molasses, 20. 0 g/L yeast extract, 15. 0 g/L K2HPO4·3H2O, 0. 2 g/L MgSO4·7H2O, 0. 01 g/L NaCl, 0. 01 g/L CaCl2·2H2O, 0. 01 g/L MnSO4·4H2O, 0. 01 g/L FeSO4·7H2O. The maximum exopolysaccharides yield of 39. 1 g/L was achieved under the following conditions: loading volume 200 mL/250 mL, inoculum size 5%, initial pH value 8. 1, fermentation temperature of 38. 5 °C and fermentation period 41. 1 h. The exopolysaccharides produced by strain M1 exhibited significant antioxidant activities, with quenching capacities of 70. 25% for DPPH radicals, 47. 16% for ·OH radicals, 35. 92% for ABTS+ radicals and 0. 42 for Fe3+ total reducing force at a concentration of 6. 0 mg/ mL. Strain M1 was capable of producing exopolysaccharides with antioxidant activity using sugar beet molasses, and could be used as a promising candidate for the bioconversion of sugar beet molasses into functional exopolysaccharides products. [ABSTRACT FROM AUTHOR]

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Copyright of Food Research & Development is the property of Food Research & Development Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Microbial exopolysaccharide (EPS) is an important biological resource, and EPS production from endophytes has attracted much attention in recent years. To explore more EPS-producing strain resources as well as soybean endophytic resources, in this study, the strain with the highest EPS yield was screened from soybean seeds, and its morphological and molecular identification, biological characteristics (temperature, pH, rotation speed, sucrose tolerance, NaCl tolerance) and physiological and biochemical studies were carried out. The results showed that the maximum EPS yield of strain SE01 was 0.63 g/L after 48 h fermentation. Colony of strain SE01 was round, with smooth edge, milky white, and glossy and sticky surface. Under microscope, the bacterium after Gram staining was short, pink and rod-shaped, which identified to Gram-negative bacterium. The results of physiological and biochemical experiments and 16S rDNA identification showed that strain SE01 was Enterobacter cloacae, and named Enterobacter cloacae SE01. The optimum growth conditions of Enterobacter cloacae SE01 were temperature 30 ℃, pH5, and rotational speed 120 r/min. The strain SE01 had high tolerance to sucrose, but low tolerance to NaCl. Increasing the concentration of NaCl could inhibit its growth. This study provides data support for the development and application of soybean endophytic EPS.

Pediococcus acidilactici was mutated by space mutagenesis technology, and the strains with high production of exopolysaccharide (EPS) were screened. The functional characteristics of the EPS were also investigated. EPS production and genetic stability of the strains were measured, and a stable mutant strain L21-49 with high production of EPS was selected. Meanwhile, the self-aggregation and hydrophobicity of the mutant strain and the original strain were investigated, and the tolerances of the strains to acid, bile salts, and artificial simulated gastrointestinal fluid were compared. The anti-biofilm, anti-oxidant, and the inhibitory activities on α-amylase and α-glucosidase of EPS were also analyzed. The results showed that the EPS yield of the mutant strain was 196.23 mg/L, which was 26.54% higher than that of the original strain L21. The strain showed good self-aggregation, hydrophobicity and tolerance in vitro. The viable cell number could be maintained at 107 CFU/mL after being cultured in pH2.0 and 3.0 for 3 h. When treated with 4.00 g/L bile salt for 24 h, the number of viable cells were 107 CFU/mL. The viable count could be maintained at 106 CFU/mL after the treatment of the artificial simulated gastrointestinal fluid. The EPS presented good antioxidant capacity in vitro. When the concentration of EPS was 8.0 mg/mL, the clearance rates on DPPH, OH, ABTS+ and \begin{document}${\rm{O}}_2^- $\end{document} were 91.75%, 38.44%, 54.71% and 58.84%, respectively. The inhibition rates of L21-49 EPS on the biofilms of Staphylococcus aureus, Listeria monocytogenes, Escherichia coli and Pseudomonas aeruginosa were 40.04%, 60.04%, 50.59% and 33.68%, respectively. It inhibited the activities of α-amylase and α-glucosidase by 23.52% and 51.15%, respectively. In this study, the effects of space mutagenesis on the probiotic characteristics and EPS production of lactic acid bacteria were investigated, so as to provide reference for the research and development of lactic acid bacteria and the EPS.

Copyright of Journal of Agro-Environment Science is the property of Journal of Agro-Environment Science Editorial Board and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In order to study the effect of polysaccharides produced by Antrodia cinnamomea in submerged fermentation on the intestinal flora of mice and, more broadly, to develop the potential and application value of A. cinnamomea in the field of functional food, we extracted and characterized intracellular polysaccharides (AIPS) and exopolysaccharides (AEPS) from the submerged cultured mycelia and broth of Antrodia cinnamomea. It was found that AIPS and AEPS were predominantly composed of glucose, galactose and mannose. Their average molecular masses were 3.52 × 106 and 4.16 × 105 Da, respectively. AEPS contained a pyran ring, while AIPS had (–C≡C–H) and (C–O) functional groups. Both AIPS and AEPS had strong digestive resistance as demonstrated by their resistance to α-amylase digestion and simulated gastric digestion. Intragastrically administered AIPS and AEPS significantly increased the relative abundance of some beneficial microorganisms (such as Lactobacillus) in the intestine of mice with lincomycin-caused diarrhea, and significantly reduced the relative abundance of some harmful microorganisms (such as Enterococcus, Staphylococcus, Parasutterella and Shigella) (P < 0.05), AEPS being more significantly better than AIPS. This study can provide a new idea and basis for the development of new multifunctional prebiotics.

The effect of addition of mixtures of exopolysaccharide (EPS) from Lactobacillus plantarum JLAU103 with fish gelatin (FG) in different proportions to low-fat yogurt on its quality characteristics was investigated. The results showed that adding 0.1% (m/m) EPS and 0.6% (m/m) FG improved the water retention and pH of low-fat yogurt, reduced the titration acidity, and resulted in viscosity similar to whole-fat yogurt. Addition of EPS and FG made the network structure of low-fat yogurt more compact, reducing the voids in it. The viable bacterial count of low-fat yogurt was significantly increased to 8.40 × 108 CFU/mL after addition of 0.6% FG and 0.1% EPS (P < 0.05). The contents of characteristic volatile flavor substances such as diacetyl, 2-nononone and 2,3-glutaredione increased significantly, the sensory score increased, and the texture was significantly improved. The rheological results showed that addition of EPS reduced the thixotropic loop area of low-fat yogurt, enhanced the stability and total strain, and caused low-fat yogurt to form a more solid gel network. Therefore, L. plantarum JLAU103 EPS combined with FG can significantly improve the quality of low-fat yogurt, and has a good application prospect.

Bacterial exopolysaccharide (EPS) is a secondary metabolite that is synthesized and secreted outside the cell wall during bacterial growth and metabolism. It can regulate the initial attachment of cells to different substrates and protect cells against environmental stress and dehydration. As a potential prebiotic, EPS has the characteristics of safety, non-toxicity and unique physical and chemical properties, and is widely used in the fields of food, medicine, biology and industry. However, the bacterial metabolic system is complex, and the biosynthetic mechanism of EPS has not been fully elucidated. Cyclic diguanylate (c-di-GMP) is an important second messenger, and it plays an important role in the regulation of many physiological activities such as biofilm formation, motility, adhesion, virulence and EPS synthesis. The analysis of the transcriptional regulation mechanism of c-di-GMP provides a new idea for exploring the biosynthesis mechanism of bacterial EPS. This paper summarizes the characteristics and synthetic degradation pathways of c-di-GMP in detail, and focuses on the regulatory mechanism of c-di-GMP in the process of bacterial EPS biosynthesis. This paper provides a theoretical basis for revealing the mechanism of bacterial EPS biosynthesis and the structure-activity relationship.

Copyright of Food Research & Development is the property of Food Research & Development Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

[Objective] Extracellular polysaccharide (EPS) producing bacteria in vivo and on body surface of some fish, shrimp and crabs in the offshore waters of Beibu Gulf of Guangxi were isolated and their biological activities were analyzed to provide a scientific basis for developing symbiotic and epiphytic microorganisms of marine animals in the coastal waters of Beibu Gulf of Guangxi. [Method] The marine bacteria were isolated from the intestinal and surface of a variety of fishes, shrimps and crabs in the area, and the EPS producing strains were screened by the mucous colorimetry. The EPS was extracted and purified by ethanol precipitation method and Sevage method, respectively. And the EPS content was determined by phenol sulfuric acid method. The EPS producing strains were preliminarily screened, bacterial species were identified and their diversity was analyzed by 16S rDNA sequence. The scavenging rate of 1, 1-diphenyl-2trinitrophenylhydrazine (DPPH) of EPS was measured and the antiviral activity of EPS against infectious bursal disease virus (IBDV) was studied to evaluate the antioxidant activity. [Result] Thirty-seven strains of EPS producing symbiotic and epiphytic marine bacteria were obtained. The 16S rDNA sequence analysis of the representative strains revealed that these strains were affiliated with 3 classes, 4 orders, 6 families, 6 genera and 10 species, respectively. Among them, the dominant genus was Bacillus, accounting for 42. 86% of the representative strains, and followed by Providencia (25.00%) and Vibrio (21.43%). The EPS content determination results showed that there were obvious differences in EPS production among different strains, and the results of DPPH clearance analysis showed that EPS produced by representative strains had certain antioxidant activity, and the DPPH clearance rates were greatly different among different strains of different genera and different species strains of the same genus. EPS produced by representative strains of Bacillus predominance had the ability to inhibit IBDV replication in the chick embryo. [Conclusion] The results indicated that there is species diversity of EPS producing bacteria from symbiotic and epiphytic microorganisms of marine animals in the Beibu Gulf of Guangxi, and the EPS produced has antioxidant and antiviral activities. The results provide a scientific basis for the utilization of the symbiotic and epiphytic bacteria resources of marine animals in Beibu Gulf of Guangxi. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Henan University of Technology Natural Science Edition is the property of Henan University of Technology Journal Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In order to expand the source of exopolysaccharides (EPS) from lactic acid bacteria, obtain EPS from lactic acid bacteria with clear source, stable yield and excellent biological characteristics. In this study, a strain of lactic acid bacteria with high EPS production was isolated and screened from self-made orange fermentation liquid by slime producing colony method. Morphological observation, 16S rDNA sequence analysis results and API 50 CHL test were combined to identify the strain. Antioxidant and milk coagulation characteristics of the lactic acid bacteria EPS were studied by antioxidant and milk coagulation test. Results showed that: A 16S rDNA fragment of Leuconostoc mesenteroides with 1444 bp was obtained, and its GenBank number was OM302141. The contents of total sugar, protein and uronic acid in strain HDE1 EPS were 41.73%±1.74%, 0.29%±0.03% and 7.69%±0.42%, respectively. The EPS showed good antioxidant activity when the concentration was 3 mg/mL. When the concentration of EPS was 5 mg/mL, the scavenging ability of DPPH, ABTS+ and H2O2- free radical reached 50.00%±0.05%, 40.00%±0.02% and more than 50%. The hydroxyl scavenging ability was 49.96%±0.03%, and the total reducing power of EPS was 38.82%±0.09%. Milk curdling results showed that HDE1 could make skim milk with 3% (w/v) sucrose coagulate completely at 36 h. These results indicated that strain HDE1 EPS had good antioxidant and milk condensation properties, showing good application potential in food, medicine and probiotics.

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Copyright of Journal of Chinese Institute of Food Science & Technology / Zhongguo Shipin Xuebao is the property of Journal of Chinese Institute of Food Science & Technology Periodical Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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