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The experiment was conducted to study the effect of probiotics, acidifiers and enzyme preparations on performance, meat quality, serum biochemical indexes and antioxidant capacity of Dagu chickens. A total of 1 200 Dagu chickens were randomly divided into five groups, with four replicates in each group and 60 chickens in each replicate. The group A (control group) was basal diet, group B was basal diet + 0.3% probiotics, group C was basal diet + 0.3% probiotics + 0.2% acidifier, group D was basal diet + 0.3% probiotics + 100 g/t enzyme preparation, and group E was basal diet + 0.3% probiotics + 0.2% acidifier + 100 g/t enzyme preparation. The experiment lasted for 63 d. The results showed that the weight gain of Dagu chickens in group C, group D and group E was significantly higher than that in control group ( P<0.05), and feed to gain radio of Dagu chickens in group C, group D and group E was significantly lower than that in control group ( P<0.05). The breast muscle shear force of Dagu chickens in group C, group D, and group E was significantly lower than that in control group ( P<0.05), the leg muscle cooking loss of large-bone chickens in group E was significantly lower than that in control group ( P<0.05), and the leg muscle shear force of large-bone chickens in group D and group E was significantly lower than that in control group ( P<0.05). The serum total cholesterol and triglyceride content of Dagu chickens in group B, group C, group D and group E were significantly lower than those in control group ( P<0.05), the serum total protein content of Dagu chickens in group D and group E were significantly higher than those in control group ( P<0.05), and the serum alanine aminotransferase activity of Dagu chickens in group E was significantly lower than that in control group (P<0.05). Serum aspartate aminotransferase of Dagu chickens in group D and group E was significantly lower than that in control group ( P<0.05). The serum MDA content of large-bone chickens in group B, group C, group D and E was significantly lower than that in control group ( P<0.05), and the T-SOD activity and T-AOC were significantly higher than those in control group ( P<0.05). The serum GSH-Px activity of large-bone chickens in group C, group D and group E was significantly higher than that in control group ( P<0.05). The experiment indicates taht probiotics, acidifiers and enzyme preparations can improve growth performance, improve meat quality, reduce blood lipid, enhance liver function and enhance antioxidant capacity of Dagu chickens. [ABSTRACT FROM AUTHOR]

The study aimed to establish the detection method of clenbuterol in lamb by using Oasis MCX SPE column combined internal standard method. The separation was performed by using acetonitrile -0.1% formic acid solution as mobile phase and 5% ammoniated methanol as eluent. The detection was conducted by using HPLC-ESI-MS/MS in positive ion and multiple reaction monitoring (MRM) modes. The results showed that this method possessed satisfactory detecting rate for clenbuterol ranging from 0.050 ng/mL to 5.000 ng/mL. The coefficient was 0.997 8, the recovery rate was 98% ~ 102%, and the relative standard deviation was 2.09%. With high sensitivity and accuracy, this method could be used for detecting the clenbuterol in lamb, and it also had certain guiding significance for quantitative analysis of clenbuterol in other meat products. [ABSTRACT FROM AUTHOR]

For the complexity, uncertainty, ambiguity grab correction system, this paper proposed fuzzy neural network based fault tree as a method for grab correction system fault diagnosis. The method extracted fault diagnosis input and output variables of grab correction system by using fault tree knowledge. It introduced the concept of fuzz logic, used fuzzy membership functions to describe the degree of failures. It used Levenberg-Marquardt algorithm to train the neural network system, got a better performance in inference speed and fault-tolerant. The experiments verify the effectiveness of fault diagnosis grab correction system based on fuzzy neural network. [ABSTRACT FROM AUTHOR]

In view of deficiencies of current column chromatography apparatus, the improvement was proposed correspondingly. The column chromatography apparatus can avoid phenomenon of dry column effectively and the requirements of purification for temperature can be satisfied by level alarm and chromatographic column with interlayer. The improved experimental device improves the efficiency of separation and purification. The practice shows that students can not only develop innovative consciousness on the common experiment for medicinal chemistry of natural products, but also set up protection consciousness of independent intellectual property during designing the device which greatly promote the organic combination of innovative experiment, design experiment and basic experiment. This pattern has certain enlightenment value for the cultivation of application and innovative pharmaceutical talents. The column chromatography device with temperature control and alarm function has characteristics of high efficiency and green, energy saving. The creation obtained the certificate of national utility model patent. [ABSTRACT FROM AUTHOR]

Addition of exogenous enzymes can significantly improve the absorption of carbohydrate and the utilization rate of protein in ruminants. However, the studies are mainly focused on cows and beef cattle, and there is a lack of more extensive research in sheep production. In the paper, the research status of exogenous enzymes, the mechanism of exogenous enzymes on ruminants, and its effects on sheep production performance, rumen microorganisms fermentation and microbial population were reviewed, to provide a reference for wider application of exogenous enzymes in sheep production. [ABSTRACT FROM AUTHOR]

In order to study the mineral properties, composition and the relationship with the organic matter characteristics in Lower Silurian Longmaxi Formation shale, 30 samples from Longmaxi Formation fresh outcrop cross sections located in Shuanghe Town, Changning County in Sichuan Basin were analyzed by scanning electron microscopy (SEM), electron probe micro analyzer, X-ray diffraction (XRD) and Cathode luminescence analysis (CL). Form the bottom up to top developed black-dark gray graptolite shale, carbonaceous shale, siliceous shale and silty shale pelitic silstone. The analysis results show that mineral compositions are brittle (fragment) minerals: quartz, feldspars, mica, pyrite; siliciclastic and calcite minerals: Chalcedony, calcite and dolomite; and clay minerals: Illite, chlorite and montmorillonite. The organic matter occurrence modes have important relationship with the mineral character. The finer fracture in the fragment minerals have huge influence to the produce and travel of the shale gas. [ABSTRACT FROM AUTHOR]

Objective To observe and explore the effects of high-flux hemodialysis (HFHD) on calcium and phosphorus metabolism in patients with maintenance hemodialysis (MHD) . Methods Eighty-six patients with MHD in our hospital from August 2012 to February 2014 were selected and randomly divided into the observation group and the control group. 43 cases in the observation group were treated with HFHD, while 43 patients in the control group were treated with low-flux hemodialysis (LFHD). All patients accepted hemodialysis for 6 months. Calcium, phosphorus, parathyroid hormone ( iPTH) and calcium-phosphorus product were measured and compared in the two groups before and after hemodialysis. Results The differences of calcium, phosphorus, iPTH and calcium-phosphorus product between two groups before treatment were not statistically significant (P > 0.05) . After hemodialysis, calcium, phosphorus, iPTH and calcium-phosphorus product were (3.2 ± 0.3) mmol/L, (1.8 ± 0.2) mmol/L, (156.5 ± 32.8) pg/mL and (32.5 ± 3.6) mg2/dL2 in the observation group, and were (2.1 ± 0.4) mmol/L, (2.1 ±0.2) mmol/L, (387.2 ± 74.5) pg/mL and (47.1 ± 4.3) mg²/dL² in the control group, respectively. The differences were statistically significant between the two groups (P < 0.05) . Conclusion HFHD can significantly eliminate phosphorus and iPTH, as well as improve serum calcium level in patients with MHD, thereby improving patient's calcium and phosphorus metabolism. [ABSTRACT FROM AUTHOR]