Search

Hyperlipidemia is closely related to renal function damage, and they influence each other. Lipid deposition in the kidney affects the function and structure of glomerulus and renal tubules directly and indirectly, resulting in renal function injury. On the contrary, renal function damage further affects lipid metabolism and aggravates the progress of hyperlipidemia. At present, besides synthetic drugs, there are many non-synthetic drugs which have been well verified in the intervention of kidney injury in hyperlipidemia animal model. In this paper, the research progress in the mechanism of renal function damage and the related intervention using hyperlipidemia animal model at home and abroad in recent years were summarized.

Objectives: To evaluate the response to cardiac resynchronization therapy (CRT) and the correlation between CRT and pulmonary artery hemodynamic parameters., Methods: The patients with chronic heart failure indicator for CRT were enrolled. The left ventricular end-systolic volume (LVESV) was measured by echocardiography and New York Heart Association (NYHA) classification was evaluated between one week before and six months after CRT. Mean pulmonary artery pressure (mPAP), pulmonary artery systolic pressure (PASP) and pulmonary vascular resistance (PVR) were measured by right heart catheterization. Left ventricular reverse remodeling (LVRR) is defined as a decrease of 15% or more in LVESV at the 6th month after CRT; Clinical response is defined as a decrease of NYHA classification at or above grade 1 at the 6th month after CRT. Pulmonary hypertension (PH) was defined as mPAP≥25 mmHg. According to the response, patients were divided into 3 groups: group A (LVRR+clinical response), group B (no LVRR+clinical response) and group C (no LVRR+no clinical response). The changes of NYHA classification, echocardiographic and pulmonary hemodynamic parameters were observed in the 3 groups. The Kaplan-Meier survival curve was used to analyze the differences in all-cause mortality, combined end-point events of death or re-hospitalization due to heart failure among different groups., Results: A total of 45 patients with CRT implantation [aged (63.27±9.55) years, 36 males] were included. The average follow-up period was (33.76±11.50) months. Thirty-one patients (68.89%) were in group A, 9 of whom with PH. Eight patients (17.78%) were in group B, 7 of whom with PH. Six patients were in group C, all with PH. Cardiac function including NYHA classification, echocardiographic and pulmonary hemodynamic parameters had been significantly improved in group A after CRT implantation ( P <0.05). In group B, NYHA classification and pulmonary hemodynamic parameters were decreased significantly ( P <0.05), but echocardiographic parameters did not change obviously ( P >0.05). There were no significant changes in NYHA classification, echocardiographic and pulmonary hemodynamic parameters in group C ( P >0.05). Compared with group C, group A and group B had lower all-cause mortality ( P =0.005) and lower incidence of composite endpoint events ( P =0.001)., Conclusions: Patients with LVRR and clinical response after CRT have a good prognosis. Patients with clinical response but without LVRR have a better prognosis than those without clinical response and LVRR, which may be related to the decrease of pulmonary hemodynamic parameters such as mPAP and TPG.

Objective: To investigate the effects of PRX-2 gene on phenotype changes in epidermal stem cells differentiating into sweat gland cells., Methods: Epidermal stem cells and sweat gland cells separated and cultured from healthy foreskin and adult full-thick skin respectively, were identified by immunofluorescence staining. Lentiviral vector-mediated overexpression and knockdown of PRX-2 gene in epidermal stem cells were performed respectively,with empty vector-mediated epidermal stem cells as a control group. Overexpression blank control and know down group's PRX-2 expressions in gene and protein levels were detected using RT-PCR and Western blot technology. The ESCs of each group were co-cultured with sweat gland cells through transwell plate, and the expressions of CEA and β1 integrin in epidermal stem cells were determined by flow cytometry before and after co-culturing., Results: Epidermal stem cells and sweat gland cells were in line with their respective specific antigens .Before co-cultured, epidermal stem cells highly expressed β1 integrin (98.69 ± 0.67)％,hardly expressed CEA (6.20 ± 3.15)％.After co-cultured,β1 integrin expression levels were showed as knockdown group (19.30 ± 0.53) ％ ＜blank control group (65.77 ± 2.32)％ ＜ overexpress group (92.63 ± 10.97)％,and CEA expression levels as knockdown (95.43 ±2.36)％ ＞ blank control group (51.20 ±0.79)％ ＞ overexpress group (45.91 ±0.93)％.There had significant differences between those of each two groups., Conclusions: PRX-2 gene can inhibit the phenotypic change of Epidermal Stem Cells differentiating into Sweat Gland Cells and improve the ability to maintain their own specific antigens.

Objective: To evaluate whether the chemokine (C-C motif) receptor 7 (CCR7) over-expressing C3H10 T1/2 mesenchymal stem cells are more efficient in immunosuppression effects than the original ones in skin grafts in mice., Methods: The C3H10 T1/2 cells were amplified and transfered with EGFP-CCR7 gene using lentivirus. The skin grafts were harvested from donators (C57BL/6 mice) and then transplanted to recipients (BALB/c mice). The recipients were divided into 4 groups randomly: CCR7⁺ C3H10 T1/2 group (1 × 10⁶ CCR7⁺ C3H10 T1/2 cells suspended in 1 mL PBS were injected into the recipient mice by caudal vein), C3H10 T1/2 group (1 × 10⁶ C3H10 T1/2 cells were injected into the recipient mice after skin graft), allogenic control group (the mice were injected with 1 mL normal saline), and congenic control group (the BALB/c mice that had received the skin from the congenic ones were injected with 1 mL normal saline). The skin graft survival condition and histopathological changes were observed on the 12th postoperative day. The Th17 cells and regulatory T cells (Tregs) in the lymphocytes of spleen were detected by mouse Th17/Treg phenotyping kit., Results: On the 12th postoperative day, the skin surface features, histopathologic changes and flow cytometry results indicated that the skin graft survival condition of the allografts treated with CCR7⁺ C3H10 T1/2 or C3H10 T1/2 cells were better than that of the allogenic control group. Compared with C3H10 T1/2 cells, CCR7⁺ C3H10 T1/2 cells had better skin graft survival condition and weaker immune response., Conclusion: CCR7⁺ C3H10 T1/2 cells induce more intensive immunosuppressive effects than original C3H10 T1/2 cells. CCR7⁺ C3H10 T1/2 cells significantly inhibit inflammatory reaction and improve the living state of skin allograft.

The combination of nanotechnology and molecular imprinting technique, including their application research in biomedical domain, provides a new solution to the problem of the substitutes for antibodies, enzymes, and other native biological structures as well as cell bracket materials. Nanocavity biomaterials with recognition specificity imprinted by using proteins as templates have numerous applications in biotechnology, medicine and so on. This review presents the aspects of molecular imprinting nanostructure involved in the biomacromolecules imprinting, and it explores the precent developments and achievements of nanomaterials for molecular imprinting technology.

In this paper, a series of low-molecular-weight PEG-PCL-PEG triblock copolymers were successfully synthesized by ring-opening polymerization method, and were characterized using 1H-NMR and FTIR. The aqueous solution displayed specific thermosensitive gel-sol transition when the concentration was above corresponding critical gel concentration (CGC). The gel-sol phase diagram was recorded using test tube-inverting method, which was depended on the hydrophilic/hydrophobic balance in macromolecular structure, as well as heating history. As a result, the gel-sol transition temperature range could be altered, which might be very useful for its application as injectable drug delivery system.

Hydrothermally synthesized nano-hydroxyapatite (n-HA) varying in weight from 10% to 30% was used as filler to make nanocomposites with novel aliphatic polyesteramide (PEA) in our laboratory. The structure and properties of PEA and its n-HA composites were investigated through transmission electron microscopy, infrared spectrometry, X-ray diffractioin, scanning electron microscopy and energy spectrometry. The shape and size of the n-HA crystals are similar to those of the apatite crystals in natural bone. Molecule interactions are present between the n-HA and PEA in the composite, which allows the uniform dispersion of n-HA in PEA matrix. This contributes enhanced mechanical property and bioactivity to the composite. The cytocompatibility of the composites has been investigated by culturing osteoblasts on the membranes. Good cell attachment and proliferation manner were observed on the membranes after 1 week. These results suggest that the PEA/n-HA composites prepared in this study may serve as potential candidate scaffold for tissue engineering.