1. [Effect of proteins extracted from mycelia of Omphalia lapidescens on inhibiting H, liver cancer in mice and regulating immune function].
- Author
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Chen YT, Lin MA, Cheng DQ, Shi ZJ, Zhu JL, and Wu J
- Subjects
- Animals, Antineoplastic Agents administration & dosage, Antineoplastic Agents pharmacology, Cyclophosphamide pharmacology, Cyclophosphamide therapeutic use, Drugs, Chinese Herbal administration & dosage, Drugs, Chinese Herbal pharmacology, Interferon-gamma blood, Interleukin-2 blood, Liver drug effects, Liver pathology, Liver Neoplasms, Experimental blood, Male, Mice, Mice, Inbred ICR, Phytotherapy, Random Allocation, Spleen drug effects, Spleen immunology, Thymus Gland drug effects, Thymus Gland immunology, Antineoplastic Agents therapeutic use, Cell Proliferation drug effects, Drugs, Chinese Herbal therapeutic use, Liver Neoplasms, Experimental drug therapy, Polyporaceae chemistry
- Abstract
Objective: To explore the effect of proteins extraceed from mycelia of Omphalia lapidescens on inhibiting H22 liver cancer in vivo., Methods: 50 hepatoma 22 tumor bearing mice models were divided into five groups randomly:control group( CG), cyclophosphamide group, and 3 groups of incremental Hepatoma 22 dosages (5, 3, 1 mg/kg). All groups were i.v. with drugs once a day. After 8 consecutive days, the concentrations of interferon-gamma (IFN-gamma) and interleukin-4 (IL-4) in serum were detected by enzyme-linked immunoabsorbent assay (ELISA). The weight changes of tumor, thymus, liver, heart, spleen, lung and kidney were observed., Results: It showed the tumors' weight were significant heavier in CG than in EGs. The tumor-inhibition rate (IR) was 36.4% in high dosage group,which was lower than 43.2% in cyclophosphamide group. The spleen mass of proteins groups increased significantly. The concentration of IFN-gamma in serum of proteins groups increased as CG, but IL-4 in inverse direction. The observations of thymus, liver, heart, lung and kidney in EGs were the same as CG., Conclusion: The proteins extracted from mycelium of Omphalia lapidescens can inhibit the growth of tumour and enhance the immune function of H22 tumor-bearing mice.
- Published
- 2009