Search

[Objective] Genetic polymorphisms determine individual genetic differences and disease susceptibility and affect drug metabolism and transport. Genetic polymorphism testing has significant application value in various fields, such as medical research, disease diagnosis, prevention, and health management. Fluorescence quantitative polymerase chain reaction (qPCR) instruments are highly sensitive and accurate molecular biology detection tools widely used in basic medical research and clinical diagnosis. Thus, medical students need to learn the fluorescence qPCR technique to improve their research capabilities and disease diagnosis levels and respond to public health events. This experiment designs three methods, namely high-resolution melting curve (HRM), allele-specific PCR (AS-PCR), and TaqMan probe, to genotype the ethanol dehydrogenase 1B (ADH1B) rs1229984 locus, aiming to guide students in using different PCR-derived techniques for genetic polymorphism testing. [Methods] The ADH1B gene sequence and the homologous genes ADH1C and ADH1A with high homology were downloaded from the GenBank database. The SnapGene software for gene alignment was used to locate the rs1229984 locus. Amplification primers for HRM-PCR, AS-PCR, and direct sequencing were designed using software such as SNPway, Primer, and Beacon Designer and algorithms such as NUPACK and MetLab. Commercial TaqMan probe primer pairs were ordered. Oral epithelial cells were successively collected from 48 volunteers, DNA was extracted using a rapid extraction method, and DNA content was measured using a spectrophotometer. PCR amplification systems were configured for the HRM, AS, and TaqMan methods, amplification reactions were performed on the corresponding PCR instruments, and the corresponding software was used for result analysis after the reaction ended. At the same time, the PCR products were detected using Sanger sequencing to identify the genotype of ADH1B rs 1229984. [Results] The HRM, AS, and TaqMan methods can all accurately identify the polymorphism of the ADH1B gene, and their genotyping results are consistent with the results of the direct and Sanger sequencing methods. Each of the three PCR methods has its advantages and disadvantages: The TaqMan method has strong sensitivity and specificity but requires designing a pair of primers in addition to a pair of locus-specific probes, which is costly. The HRM method is the simplest to operate and the lowest in cost, but it requires high-quality DNA. The AS method has strong specificity but requires high primer design standards and involves PCR amplification reactions with two upstream primers and one downstream primer, comparing the ΔCt values of the two for judgment, which is relatively complex. [Conclusions] By establishing the HRM, AS, and TaqMan methods, the polymorphism of the ADH1B gene rs122998 locus in 48 volunteers was successfully genotyped. The accuracy of the experimental results confirmed the reliability of fluorescence qPCR technology in the analysis of genetic polymorphisms. Through experimental teaching, students can be taught to apply what they have learned to detect other genetic polymorphisms. When choosing a detection method, several factors, such as the purpose of the experiment, number of samples, cost, and ease of operation, need to be considered comprehensively. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Henan Agricultural Sciences is the property of Editorial Board of Journal of Henan Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: The essence of the individual genetic differences of human leukocyte antigen (HLA) is the new alleles generated under various inducing factors at the level of genes encoding its antigen products, the frequency distribution in the population, and the blood type of the antigen. Serological testing and biological functions need to be further studied. OBJECTIVE: The identification and sequence analysis of a new HLA allele HLA-DRB1*12:02:10. METHODS: The polymerase chain reaction-sequence-based typing, single-strand sequencing, and nex-generation sequencing were used to identify the HLA-DRB1 non-matched transplant match samples. The control experiment was performed by polymerase chain reaction-sequence specific oligonucleotide probe. RESULTS AND CONCLUSION: (1) The HLA-DRB1 had T>G; a base mutation in Exon 3 at position 582, which caused the 194 codon of Exon 3 to be changed by CCT>CCG, which was a synonym mutation without any change of amino acid. (2) The new HLA-DRB1 allele was identified and named HLA-DRB1*12:02:10 by the World Health Organization HLA Factor Nomenclature Committee. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Modern Laboratory Medicine is the property of Journal of Modern Laboratory Medicine Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Modern Laboratory Medicine is the property of Journal of Modern Laboratory Medicine Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Forensic Medicine / Fayixue Zazhi is the property of Journal of Forensic Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

[Objective] This study explored SSR loci associated with main economic characters of Ziziphus jujuba var. spinosa to provide basis for gene mining, excellent parent breeding and resource exploitation of Z. jujuba var. spinosa. [Method] The single fruit mass, fruit horizontal diameter, fruit vertical diameter, fruit-form index, single nutlet mass, nutlet horizontal diameter, nutlet vertical diameter, nucleation index, single kernel mass, kernel horizontal diameter, kernel vertical diameter, kernel-form index, kernel side diameter, dual kernel rate, edible rate, nutlet rate and kernel rate of 72 Z. jujuba var. spinosa germ-plasms in western Liaoning were investigated. SPSS 22. 0 software was used for statistical analysis of economic traits, 32 SSR markers were selected, and Structure 2. 3. 4 software was used to analyze the population structure of germplasms. Tassle 2. 0 software was used to analyze the linkage disequilibrium among 32 SSR markers. General linear model (GLM) and mixed linear model (MLM) were used to analyze the association between economic traits and SSR markers, and the phenotypic effects of alleles at association sites were analyzed to obtain typical vector materials. [Result] The variation coefficients of the 17 economic traits of 72 Z. jujuba var. spinosa germplasms ranged from 7. 84% to 85. 75 %, indicating that the tested germplasms had rich phenotypic diversity. The 72 Z. jujuba var. spinosa germplasms were divided into 4 groups by population genetic structure analysis, indicating unique population genetic background. The level of linkage disequilibrium among 32 SSR markers was low. There were 166 pairs of unbalanced combinations, accounting for 33. 47% of the total number of combinations. In the correlation analysis, 21 SSR loci significantly associated with 17 major economic traits were detected by the GLM model (P<0.05), and the explained rate of variation was 15. 35% --69. 14%. The MLM model detected 17 SSR loci significantly associated with 16 economic traits and the explained rate of variation was 0.82% -- 75. 37%. The phenotypic effect values of each allelic fragment of 16 SSR association sites detected in the two models were analyzed, and 31 excellent allelic variations with maximum efficiency increase and reduction and 15 typical carrier materials were excavated. [Conclusion] Based on SSR correlation analysis, 16 SSR association site and 15 aggregated excellent alleles associated with 16 economic traits were selected. [ABSTRACT FROM AUTHOR]

Copyright of Mycosystema is the property of Mycosystema Editorial Board and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

【Objective】By detecting the natural variation of Auxin Up-regulated F-box protein2 (AUF2) gene and exploring the elite alleles associated with agronomic traits in order to provide theoretical references for molecular marker assisted breeding of flowering Chinese cabbage.【Method】PCR amplification and sequencing was used to detect the natural variations in AUF2 gene among 156 flowering Chinese cabbage accessions. By using the mixed linear model (MLM) of Tassel 5.0 software, the correlation between the variation sites of AUF2 gene and six agronomic traits of flowering Chinese cabbage was analyzed, including plant height, single plant weight, maximum leaf length, maximum leaf width, maximum petiole length and chlorophyll content (SPAD), and the polymorphic sites significantly associated with agronomic traits would be identified. Then, elite alleles and excellent haplotypes were determined.【Result】Through sequencing analysis, a total of 34 mutation sites were detected in the 2 996 bp amplification region of AUF2 gene, including 36 haplotypes. The AUF2 gene showed high nucleotide diversity (π = 0.00503) and haplotype diversity (Hd = 0.780). Among them, 2 InDels and 26 SNPs were in the 1 498 bp upstream non-conding region, and 1 InDel and 5 SNPs were in the 960 bp coding region. No mutation sites were detected in the 538 bp downstream non-conding region. Neutral test analysis showed that Tajima’s D value, Fu and Li’s D* value, and Fu and Li’s F* value of the AUF2 gene were positive, and deviated significantly from neutral evolutionary model. One plausible reason was that flowering Chinese cabbage population was subject to balancing selection. The association analysis showed that 4 SNPs were significantly associated with at least one of the four agronomic traits, including single plant weight, maximum leaf length, maximum leaf width and maximum petiole length. The phenotypic variation of a given trait explained by each associated SNP ranged from 5.04% to 6.52%. The haplotype Hap2, which contained elite alletes of the four SNPs mentioned above, could significantly raise the single plant weight, and remarkably increase the length of the maximum petiole.【Conclusion】There are abundant variations in the AUF2 gene, which has a significant impact on the agronomic traits of flowering Chinese cabbage. These elite alleles and excellent haplotypes of AUF2 gene will benefit the genetic improvement of flowering Chinese cabbage. [ABSTRACT FROM AUTHOR]

The purpose of this study was to investigate the genetic diversity, the microevolutionary process and the evolutionary history of human-type Ascaris, pig-type Ascaris, and hybrid Ascaris and to provide a theoretical basis for the prevention and control of human and animal ascariasis. Results on genetic diversity of human-type Ascaris, pig-type Ascaris and hybrid Ascaris based on microsatellite markers in this study showed that a total of 130,51,42 alleles were detected in human-type Ascaris, pig-type Ascaris and hybrid Ascaris, respectively. The advantage alleles and the private alleles varied in three populations. The average expected heterozygosity and polymorphism information content revealed rich genetic diversity. The highest genetic diversity was found in the human-type Ascaris population, while the lowest was found in the pig-type Ascaris population. AMOVA analysis showed that the genetic variation of human-type, pig-type, and hybrid Ascaris populations mainly came from individual populations, and the degree of genetic differentiation among the three populations was moderate. Gene flow analysis manifested that there were different levels of gene exchange between populations. The gene exchange between human-type Ascaris and hybrid Ascaris was more abundant. In addition, the data suggest that there are differences between hybrid Ascaris, human- type Ascaris, and pig-type Ascaris. This research not only has important academic significance, but also has potential application prospect. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Agricultural Science & Technology (1008-0864) is the property of Journal of Agricultural Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Oil Crop Sciences is the property of Oil Crops Research Institute of Chinese Academy of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Oil Crop Sciences is the property of Oil Crops Research Institute of Chinese Academy of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Liquidambar formosana is one of the important native tree species in Guangxi, which has high timber, ornamental and medicinal value. This study designs and develops EST-SSR markers of L. formosana based on data from the transcriptome sequencing. Primers were developed, and screened out by PCR amplification and polyacrylamide gel electrophoresis for high polymorphism, and the efficiency was tested by using 30 individuals from a wild L. formosana population to verify the practical application of these SSR primers. The results were as follows: (1) A total of 23 777 SSR loci were obtained by searching SSR Unigenes from transcriptome data. The repeat type of the SSR loci in L. formosana was dominated by mononucleotide repeat type (46.54%). The highest proportion of SSR loci (72.36%) was between 5 and 12 times. (2) A total of 262 pairs of SSR primers were developed, and among them, 139 pairs of effective amplifications with a success rate of 53.1%, and 18 pairs of them that could be used to steadily obtain clear bands were finally identified. (3) The polymorphism detection showed that all sites had a high degree of polymorphism. The number of alleles (Na), effective alleles (Ne), Shannon's diversity index (I), observed heterozygosity (Ho) of the L. formosana population ranged in 2-4, 1.112 8-2.609 6, 0.208 9-1.112 7 and 0.275 9-1.000 0, the average values were 2.333 3, 1.957 4, 0.708 5 and 0.722 6, respectively. In conclusion, the dominant SSR repeat type and repeat motif in L. formosana are basically the same as those in other species, the developed 18 pairs of EST-SSR markers can meet the needs of population genetic studies of L. formosana, which provides abundant primers for the study of genetic diversity of L. formosana. It is of important significance to the protection, development and utilization of L. formosana. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Modern Laboratory Medicine is the property of Journal of Modern Laboratory Medicine Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The study was to study the effect of prolactin receptor (PRLR) gene SNPs polymorphism on reproductive performance in Shenxian pigs. The SNPs locus of PRLR gene was detected by PCR-RFLP technique combined with mixed pool generation sequencing technology, and association with the reproductive performance of 71 multiparous sows of shenxian pigs was analyzed. The results showed that there were two SNPs loci of PRLR gene in Shenxian pig population: A133T and T225C, both located in exon 8. There were three genotypes at A133T: AA genotype, AT genotype and TT genotype, in which AA genotype was the dominant genotype and A is the dominant allele. The total litter size, litter weight and live litter size of AA genotypes were significantly higher than those of TT genotypes (P<0.05). There were three genotypes at T225C: TT, TC and CC, in which C was the dominant allele and CC was the dominant genotype. The total litter size and litter weight of TC and CC genotypes were significantly higher than those of TT genotypes (P<0.05). Among the combined genotypes, ATTC and ATCC genotypes had higher reproductive performance as a whole. The two loci were in Hardy-Weinberg equilibrium. The polymorphism information content was 0.329 and 0.373, respectively. The heterozygosity was 0.416 and 0.495, and the homozygosity was 0.584 and 0.505, respectively. The study indicates that the reproductive performance of Shenxian pigs can be improved by selecting individuals with AA genotypes at A133T site and TC genotypes at T225C loci, and combining individuals with ATTC and ATCC genotypes. [ABSTRACT FROM AUTHOR]

Copyright of South China Fisheries Science is the property of South China Fisheries Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Forensic Science & Technology is the property of Institute of Forensic Science, Ministry of Public Security and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: The essence of the individual genetic differences of human leukocyte antigen（HLA） is the new alleles generated under various inducing factors at the level of genes encoding its antigen products, the frequency distribution in the population, and the blood type of the antigen. Serological testing and biological functions need to be further studied.OBJECTIVE: The identification and sequence analysis of a new HLA allele HLA-DRB1*12:02:10.METHODS: The polymerase chain reaction-sequence-based typing, single-strand sequencing, and nex-generation sequencing were used to identify the HLA-DRB1 non-matched transplant match samples. The control experiment was performed by polymerase chain reaction-sequence specific oligonucleotide probe. RESULTS AND CONCLUSION:（1） The HLA-DRB1 had T>G; a base mutation in Exon 3 at position 582, which caused the 194 codon of Exon 3 to be changed by CCT>CCG, which was a synonym mutation without any change of amino acid.（2） The new HLA-DRB1 allele was identified and named HLA-DRB1*12:02:10 by the World Health Organization HLA Factor Nomenclature Committee. [ABSTRACT FROM AUTHOR]

Copyright of Forensic Science & Technology is the property of Institute of Forensic Science, Ministry of Public Security and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Tea Science is the property of Journal of Tea Science Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objetive: To analyze the sequence of a novel HLA-DPB1 allele in an individual., Methods: A individual identified from the database of blood donors for matched platelet transfusion at the Blood Center of Zhejiang Province in May 2022 was selected as the study subject. HLA genotype of the individual was determined by next-generation sequencing (NGS) on an Ion Torrent S5 platform. The sequence of the HLA-DPB1 locus was also determined by NGS on an Illumina Miseq platform and third-generation sequencing using Oxford Nanopore MinION. This study was approved by the Blood Center of Zhejiang Province (Ethics No. 2021-001)., Results: A novel HLA-DPB1*02 allele was identified in the specimen, for which the closest genotype was HLA-DPB1*02:new,17:01:01G, with the variant located in exon 3. Meanwhile, the NGS also revealed a novel HLA-DPB1*17 allele, with the closest genotype being HLA-DPB1*02:01,17:new. Both the HLA-DPB1*17:01:01:01 and HLA-DPB1*02 alleles were identified by third-generation sequencing. Compared with the HLA-DPB1*02:01:02:01 allele, the novel allele had a G>A variation at position 369 in the exon 3, which however did not result in amino acid change., Conclusion: A novel HLA-DPB1 allele has been identified and validated by both NGS and TGS, which has been named as HLA-DPB1*02:01:69 by the World Health Organization Committee on Nomenclature of Factors of the HLA System.

Objective: To analyze the distribution of clopidogrel metabolism-related gene variability in Kawasaki disease (KD) children with coronary artery lesions (CAL) across different age groups and the impact of genetic variability on the efficacy of clopidogrel antiplatelet therapy. Methods: A retrospective cohort study was conducted. Clinical data were collected from 46 KD children with CAL who were hospitalized in the Cardiovascular Center of Children's Hospital of Fudan University between January 2021 and August 2022 and were treated with clopidogrel, including gender, age, body mass index, course of KD, CAL severity grade, and baseline platelet count. According to their age, the children were divided into ≥2-year-old group and <2-year-old group. Their platelet responsiveness was assessed by adenosine diphosphate-induced platelet inhibition rate (ADPi) calculated via thromboelastography, and children were categorized into high on-treatment platelet reactivity (HTPR) and normal on-treatment platelet reactivity (NTPR) groups. Genotypes of CYP2C19, PON1 and ABCB1 were detected. The t test, one-way analysis of variance and Chi-square test were used for intergroup comparison. Results: Among the 46 KD children with CAL, 34 were male and 12 were female; 37 were ≥2-year-old and 9 were <2-year-old; 25 cases were in the HTPR group and 21 cases were in the NTPR group, with 19 HTPR and 18 NTPR in the ≥2-year-old group, and 6 HTPR and 3 NTPR in the <2-year-old group. Genetic analysis showed that 92 alleles among the 46 children, with frequencies of CYP2C19*1, CYP2C19*2, CYP2C19*3, CYP2C19*17, PON1 192Q, PON1 192R, ABCB1 3435C, ABCB1 3435T at 59% (54/92), 32% (29/92), 9% (8/92), 1% (1/92), 36% (36/92), 64% (59/92), 63% (58/92) and 37% (34/92), respectively. Analysis of the impact of genotype on ADPi revealed that in children aged ≥2 years, those with CYP2C19*1/*3 genotype had significantly lower ADPi than those with CYP2C19*1/*1 genotype ((34±15)% vs. (61±29)%, t =2.18, P =0.036). There were also no significant difference in ADPi among children with PON1 192Q homozygous, PON1 192R heterozygote and PON1 192R homozygous genotypes ((40±22)% vs. (52±33)% vs. (65±27)%, F =2.17, P =0.130), or among those with ABCB1 3435C homozygous, ABCB1 3435T heterozygote and ABCB1 3435T homozygous genotypes ((55±34)% vs. (60±27)% vs. (49±24)%, F =0.33, P =0.719). In <2-year-old group, there were no significant differences in ADPi across CYP2C19*1/*1, CYP2C19*1/*2 and CYP2C19*2*2 genotypes ((40±20)% vs. (53±37)% vs. (34±16)%, F =0.37, P >0.05). There were no significant differences in ADPi across CYP2C19*1/*1 and CYP2C19*1/*3 genotypes ((44±27)% vs. (42±20)%, t =0.08, P >0.05). There were no significant differences in ADPi across PON1 192Q homozygous, PON1 192R heterozygote and PON1 192R homozygous genotypes (45% vs. (55±27)% vs. (24±5)%, F =1.83, P >0.05). There were no significant differences in ADPi across ABCB1 3435C homozygous, ABCB1 3435T heterozygote and ABCB1 3435T homozygous genotypes ((36±16)% vs. (50±35)% vs. 45%, F =0.29, P >0.05). The risk analysis of HTPR in different genotypes revealed that in children aged ≥2 years, carrying at least 1 or 2 loss-of-function alleles of CYP2C19 was a risk factor for HTPR ( OR =4.69, 10.00, 95% CI 1.11-19.83, 0.84-119.32, P =0.033, 0.046, respectively), and PON1 192R homozygosity and carrying at least one PON1 192R allele were protective factors against HTPR ( OR =0.08, 0.13, 95% CI 0.01-0.86, 0.01-1.19, P =0.019, 0.043, respectively). Conclusion: KD children aged ≥2 years carrying CYP2C19 loss-of-function alleles and PON1 192Q are more likely to develop HTPR.

Objective: To investigate the molecular mechanism and distribution characteristics of RhD negative phenotypes in Han population of blood donors in Wuhu city., Methods: A total of 210 RhD - samples from August 2021 to August 2022 were screened by serological test and collected from Wuhu Central Blood Station for the voluntary blood donor population. Exons 1 and 10 of the RHD gene were amplificated by PCR to determine whether the samples had the RHD gene. Exons 1-10 of the RHD gene were amplificated by PCR and zygosity analysis were performed in 82 samples containing D gene, and Sanger sequencing was performed on 55 samples containing all RHD exons to determine the genotype., Results: Among 210 RhD - specimens, 128 cases (60.38%) had RHD gene deletion. 27 cases had partial exons of RHD , including 2 cases with RHD*DVI.3/RHD*01N.01 , 24 cases with RHD*01N.04/RHD*01N.01 , and 1 case with RHD-CE(2-10)/RHD*01N.01 . 55 cases had retained all of 10 exons, including 4 cases with RHD*01/RHD*01N.01 , 6 cases with RHD*15/RHD*01N.01 , 1 case with RHD*01W.72/RHD*01N.01 , 1 case with RHD*15/RHD*01EL.01 , 39 cases with RHD*01EL.01/RHD*01N.01 , and the remaining 4 cases were determined to have no RHD gene deletion by zygosity analysis and sequencing showed the presence of 1227G>A mutation loci., Conclusion: There is polymorphism in the molecular mechanism of RhD - D gene in Wuhu blood donor population, among which RHD*01EL.01 and RHD*15 are the main variants in this region. The results of this study provide a theoretical basis for RhD blood group identification and clinical blood transfusion in this region.

Objective: To explore the Rh genotype for a female with RhD(-) blood type and its molecular basis., Methods: A 26-year-old female who had attended the outpatient clinic of the First Affiliated Hospital of Zhengzhou University in August 2019 was selected as the study subject. Peripheral blood samples were collected from the proband and her parents for Rh phenotyping with gel card method. PCR-sequence-based typing (PCR-SBT) and DNA sequencing were used to determine the RHD zygosity and Rh genotype of the proband and her parents. Homology modeling of Rh proteins was performed with bioinformatic software, and protein structural alterations caused by the variant was simulated by molecular dynamics., Results: Serological tests showed that the proband and her father both had weakened e antigen of the Rh phenotype. PCR-SBT and DNA sequencing showed that the genotypes of the proband and her parents were dce/dCE, dce/DcE and dCE/DcE, respectively. And the genotypes of the RHD and RHCE of the proband were RHD*01N.01/RHD*01N.16, RHCE*01.01/RHCE*04, respectively. Protein simulation and molecular dynamics analysis revealed that the ce&#95;16C variant resulted from RHCE*ce (c.48G>C) may alter the structure of intracellular and extracellular loops, mainly affecting the mobility of extracellular loops 2, 6 and intracellular loops 3, 4., Conclusion: Variant of the RHCE*ce allele c.48G>C probably underlay the weakened e antigen in this proband.

Copyright of Journal of Henan Agricultural Sciences is the property of Editorial Board of Journal of Henan Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: To explore and analyze the correlation between serum IL-8 gene polymorphism and ESCC after radical resection. Methods:From August 2017 to June 2020, 98 cases of patients with esophageal squamous cell carcinoma diagnosed and treated in our hospital were selected as the research object, and the serum IL-8 expression level were detected. All patients were treated with radical surgery, and the prognosis of the patients were followed up and given correlation analysis. Results: All patients were followed up to July 2021. The average follow-up time were 25.69± 2.58 months. There were 28 deaths and the mortality rate were 28.6 % (death group). The expression level of serum IL-8 in the death group were higher than that in the survival group (P<0.05). The genotype frequencies of all patients were conformed to the Hardy-Weinberg equilibrium rule, which confirmed that the samples were representative of the population. The AA genotype of the IL-8 gene promoter rs4073A/T were higher than that of the death group(P<0.05), and the TT genotype were lower than that of the death group (P<0.05). There were also statistical differences in the frequency distribution of A and T alleles compared between the two groups (P<0.05). Linear correlation analysis showed that the AA genotype and A allele of IL-8 gene promoter rs4073A/T, expression level of serum IL-8 were correlated with prognosis and mortality(P<0.05). Multivariate logistic regression analysis showed that the AA genotype and A allele of IL-8 gene promoter rs4073A/T, expression level of serum IL-8 were the main factors leaded to followed-up death (OR=2.051, 3.094, P<0.05). Conclusions: Patients with esophageal squamous cell carcinoma still have certain mortality rate after radical resection. There are correlation between the death of patients and the polymorphism of serum IL-8 gene. It are also often accompanied by high expression of IL-8. The AA genotype and A allele of IL-8 gene promoter rs4073A/T, expression level of serum IL-8 are The main factor lead to the death of patients. [ABSTRACT FROM AUTHOR]

Copyright of Forensic Science & Technology is the property of Institute of Forensic Science, Ministry of Public Security and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Henan Agricultural Sciences is the property of Editorial Board of Journal of Henan Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of South China Fisheries Science is the property of South China Fisheries Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

[Objectives]This article aimed to study the genotypes and haplotypes of MYB(myeloblastosis)regulatory genes in wine grapes(Vinifera), and to analyze the relationship between fruit color and haplotype composition and quantity. [Methods]The specific primers were used to identify the genotype and haplotype composition of the MYBA1 and MYBA2 gene loci of 72 wine grape varieties by PCR technology, and the color of the fruit was investigated at the same time. [Results]PCR results showed that at the MYBA1 locus, VvmybA1a was detected in 56 varieties, VvmybA1b was detected in only 1 variety, VvmybA1c was detected in 16 varieties, VlmybA1-3 was detected in 13 varieties, VvmybA1SUB was detected in only 3 varieties, and VvmybA1BEN was not detected in all 72 varieties. At MYBA2 locus, VlmybA1-2 was detected in 6 hybrid varieties of Vitis vinifera×V.labrusca, VlmybA2 was detected in 3 hybrid varieties V.vinifera×V.labrusca, VvmybA2r was detected in 29 varieties, and VvmybA2w was detected in 58 varieties. There were 16 haplotype combinations of A, G, AB, AC-N, AC-NE1, AC-Rs, AE1, AE1E2, AF, C-N, C-NE1, C-Rs, CE1, C-RsF, C-RsE1 and GF. The grape skins containing the haplotype HapC-N were mostly purple black or blue-purple, which was the most common combination among the haploid composition types of colored varieties. The haplotype combination with the largest number of colored grape varieties in V.vinifera was AC-N, and the haplotype combination with the largest number of colored grape varieties in hybrids of V.vinifera×V.labrusca was AE1. The haplotype combinations of most colored grapes in V.vinifera were AC-N, while the number of haplotype combinations of AC-N, C-Rs and AE1E2 in hybrids of V.vinifera×V.labrusca was equivalent. [Conclusions]The MYB gene structure of wine grapes was highly conserved, a total of 10 alleles at 2 gene loci form different haplotypes participated in the regulation of fruit coloration. The number of haplotypes was not directly related to the peel color, only one coloring gene varieties might be purple black, while varieties with more than 2 coloring genes might have red peel color; different environmental conditions might also cause changes in peel color. [ABSTRACT FROM AUTHOR]

[Objective] In order to provide theoretical basis for crossing and molecular marker assisted breeding of plateau japonica rice, the genetic structure and SSR markers associated with agronomic traits were analyzed. [Method] The agronomic characters of 81 plateau japonica rice cultivar was investigated under two altitude conditions, and 48 SSR markers were used to detect the polymorphism and analyze the genetic structure, and then the association between SSR markers and agronomic traits was analyzed by the 2.1 MLM (Mixed Linear Model) method. [Result] There were a total of 139 allele tested by 37 SSR markers with polymorphic among 81 varieties, the allele ranging from 2 to 10, with an average of 3.76. The tested varieties were divided into two sub-populations by population genetic structure analysis. Association analysis showed that RM1195 and RM209 were associated with plant height and 1000-grain weight, respectively. While RM267, RM332, RM490, RM583 and RM590 were associated with seed setting rate at the level of P < 0. 05 under both conditions, and the rate of explanation on the phenotype of related marker ranged from 0.074 to 0.352. However, only RM332 was associated with seed setting rate at the level of P < 0.001, and the SSR markers was located on chromosome 11. [Conclusion] These markers detected in the study provide theoretical basis for crossing and molecular marker assisted breeding of plateau japonica rice. [ABSTRACT FROM AUTHOR]

Research of the population structure and genetic diversity has guiding significance to soybean improvement. In this study, 105 cultivar soybean accessions bred from Huang-Huai-Hai and Southern region of China were collected to detect their population structure and genetic diversity by using 99 SSR polymorphic primer pairs. The results showed that a total of 1142 alleles were detected at 99 loci with 11.54 average number of alleles per locus ranging from 5 to 24. The whole population was divided into 4 subgroups according to breeding period. The range of Nei's gene diversity was 0.628-0.839 with an average of 0.774. The polymorphism information content ranged from 0.562-0.820 with an average of 0.742. The genetic distance between subgroups ranged from 0.387 to 0.197 with an average of 0.297. Ninety-nine percentage of total variation was explained within the subgroups, and 1% of total variation was explained among subgroups based on the molecular variation analysis, which indicated that there were frequent gene exchanges in subgroups. The principal coordinate analysis showed that the first, second, and third principal factors explained 4.12%, 3.61%, and 2.90% of the total variation respectively. These soybean germplasm could be divided into 3 subgroups based on STRUCTURE analysis and unweighted pair group method with arithmetic average (UPGMA) cluster. Further comparison showed that the UPGMA subgroups and STRUCTURE subgroups displayed a highly consistent correlation with these pedigrees and the genetic base of each period. The genetic diversity had period characteristics and showed an increasing trend in this study. [ABSTRACT FROM AUTHOR]

[Objective] The aim of this study was to explore the differences between morphological traits and SSR marker identification, to provide theoretical basis for the improvement of maize SSR marker identification procedures. [Method] Using 20 pairs of core primers screened from SSR marker identification protocol to analyze the distinctness and diversity of 45 maize hybrid varieties. At the same time, 38 characteristics essential to be tested were tested of 45 maize hybrids. Based on morphological traits, distinctness and diversity of 45 maize hybrid varieties were analyzed. The relationship between morphological trait identification and SSR marker identification was compared and analyzed. [ Result] The results of SSR marker identification were consistent with morphological trait testing for 45 maize hybrid varieties, and there were distinctness among 45 maize hybrid varieties. 218 allele variations were detected with 20 SSR core primers for 45 maize hybrids, with an average of 10. 90 per pair; The average effective allele variations were 3. 1600; And the average Shannon-Weaver diversity information index was 2. 8922. Total 178 allelic variations were detected for 38 characteristics essential to be tested in 45 maize hybrid varieties, with an average of 4. 6842 for each trait; An average Shannon-Weaver diversity information index 1. 2593; And an average effective allelic variation 2. 7830. SSR markers and morphological traits clustering analysis showed that the similarity coefficients of 45 maize hybrid varieties were 0. 62 -0. 94 and 0. 31 -0. 68,respectively. 45 maize hybrid varieties could be divided into three groups,and the similarity was high between two clustering results. [ Conclusion] The molecular differences value between two random maize hybrids was 1. 94 times the morphological difference value. It was suggested that criterion value of molecular differences should be greater or equal to 4 when SSR marker identification was used to determine distinctness for two hybrids. [ABSTRACT FROM AUTHOR]

Objective: To assess the association of cytotoxic T lymphocyte-associated antigen-4 (CTLA-4) gene polymorphisms with the prognosis of patients with Bladder urothelial carcinoma (BUC)., Methods: From February 2019 to October 2020, 256 BUS patients treated at the Xinxiang Central Hospital were selected as the study group, whilst 250 healthy individuals were selected as the control group. Genotypes of rs5742909 (-318C/T), rs231775 (+49A/G) and rs4553808 (-1661A/G) were determined by PCR-restriction fragment length polymorphism assay. The frequencies of genotypes and alleles of the CTLA-4 gene were compared between the two groups. All patients had undergone surgical treatment and were followed up for 3 years and divided into good prognosis group (n = 166) and poor prognosis group (n = 86) based on the status of disease. The distribution of alleles and genotypes were compared, and Kaplan-Meier analysis was used to assess the association of genetic polymorphisms with the prognosis., Results: No significant difference was found in the gender, age, BMI, smoking history and alcohol use between the two groups (P > 0.05). The frequencies of GG genotype and G allele for the rs231775 (+49A/G) and rs4553808 (-1661A/G) loci were significantly higher in the study group compared with the control group (P < 0.05), whilst no statistical difference was found in the genotypic and allelic frequency for the rs5742909 locus between the two groups (P > 0.05). Among the 252 subjects who had completed follow-up, 86 had poor prognosis and 166 had good prognosis. The frequencies of GG genotype and G allele at the rs231775 (+49A/G) and rs4553808 (-1661A/G) loci were significantly lower in the good prognosis group compared with the poor prognosis group (P < 0.05). Kaplan-Meier survival curve analysis showed that the survival time of patients with GG genotype for the rs231775 (+49A/G) and rs4553808 (-1661A/G) loci was significantly shorter than patients with AA or AG genotypes (Log Rank 2 = 13.654, 9.974, P < 0.001)., Conclusion: The polymorphisms of the rs231775 and rs4553808 loci of the CTLA-4 gene are associated with genetic susceptibility and poor prognosis for BUC, and a higher GG genotypic frequency may increase the risk for infection and poor prognosis of the patients.

Objective: To explore the serological characteristics and genetic variant in a Chinese pedigree with Bw subtype., Methods: A 32-year-old female proband who had undergone prenatal examination on December 10, 2020 at the 960th Hospital of the PLA Joint Logistics Support Force and five members from her pedigree were selected as the study subjects. Peripheral blood samples were collected and subjected to ABO blood group phenotyping with serological methods and ABO blood group genotyping with fluorescent PCR. Genetic testing and haplotype analysis were carried out by direct sequencing of the entire coding region of the ABO gene in the proband and cloned sequencing of exons 1-7., Results: The blood type serology of the proband showed Bw, and her ABO blood type genotype determined by fluorescence PCR was B/O. The direct sequencing results showed that the proband had matched the ABO*O.01.01/ABO*B.01 genotype and carried a c.1A>G variant. Cloned sequencing has confirmed the c.1A>G variant to have occurred in the ABO*B.01 allele. Family analysis revealed that the mother of the proband had an O blood type, her husband had a B phenotype, and her three children had a normal B blood type. DNA sequencing showed that the two sons of the proband had a genotype of ABO*B.01 and c.1A>G/ABO*B.01. The daughter of the proband was ABO*O.01.01/ABO*B.01, whilst her mother was ABO*O.01.01/ABO *O.01.02. The novel c.1A>G variant sequence has been registered with the database with a number MZ076785 1., Conclusion: The novel c.1A>G variant of exon 1 of α- 1,3 galactose aminotransferase gene probably underlay the reduced expression of B antigen in this pedigree.

Objective: To explore the serological characteristics and molecular mechanism underlying an individual with A3 phenotype., Methods: A 27-year-old ethnic Han Chinese woman presented at the Fourth Affiliated Hospital of China Medical University on May 12, 2022 was selected as the study subject. ABO blood type was determined with standard serological techniques. The ABO gene was subjected to direct sequencing of PCR products. Exons 6 and 7 of the ABO gene were sequenced using specific primers to determine the haplotypes. Bioinformatic software was used to analyze the structure of the mutant protein., Results: Serological typing of the ABO blood group has suggested a rare A3 phenotype. The proband was found to harbor heterozygous c.261delG, c.467C>T and c.745C>T variants by direct sequencing. Single strand sequencing revealed that she has harbored ABO*A3.07 and ABO*O.01.01 alleles. The ABO*A3.07 allele has contained a c.745C>T (p.R249W) variant on the background of an ABO*A1.02 allele. The p.R249W substitution was predicted to be probably damaging by the PolyPhen2 software. The free energy change (ΔΔG) value predicted it to have a destabilizing effect on the GTA protein. Meanwhile, modeling of the 3D structure has predicted that the p.R249W amino acid substitution may alter the hydrogen bond network of the GTA protein., Conclusion: The p.R249W substitution of the α-1,3-N-acetylgalactosaminyltransferase gene may reduce the antigen expression owing to a great destabilizing effect on the structure and function of the GTA protein.

Objective: To investigate the relationship between the polymorphism of endoplasmic reticulum aminopeptidase 1 (ERAP-1) gene and the occurrence of pre-eclampsia (PE). Methods: A case-control study was conducted in Beijing Obstetrics and Gynecology Hospital from October 2018 to October 2021. A total of 51 PE pregnant women with onset gestational age<34 weeks were selected as the PE group, and 48 normal pregnant women during the same period were selected as the control group. Venous blood samples were collected from the pregnant women before delivery and umbilical cord within 5 minutes after delivery. Single nucleotide polymorphisms (SNP) of ERAP-1 gene in the pregnant women and their fetus were detected by next-generation sequencing. Univariate analysis and multivariate logistic regression analysis were used to analyze all the SNP loci and alleles detected in the two groups, and the significant SNP were screened. Results: (1) A total of 13 target SNP loci of maternal ERAP-1 gene were selected by univariate analysis. Among them, the frequency distribution of genotypes at 96096828, 96121524, 96121715, 96122260 and 96122281 showed statistically significant differences between PE group and control group (all P <0.05). Multivariate logistic regression analysis showed that the risk of PE in pregnant women with TC genotype at locus 96121524 was 2.002 times higher than those with TT genotype (95% CI : 0.687-5.831, P =0.020). (2) A total of 4 target SNP loci of ERAP-1 gene in fetal were selected by univariate analysis, and there was no statistical significance in gene polymorphism of the 4 loci between PE group and control group (all P> 0.05). Multivariate logistic regression analysis showed that the risk of PE in fetus with genotype AA at locus 96121406 was 0.236 times that of fetus with genotype GG (95% CI : 0.055-1.025, P =0.016). Conclusion: ERAP-1 gene with TC genotype at 96121524 in the mother and GG genotype at 96121406 in the fetus might be related to the incidence of PE.

Objective: To explore the characteristics of phenylalanine hydroxylase (PAH) gene variants and prenatal diagnosis for 43 Chinese pedigrees affected with Phenylketonuria (PKU)., Methods: Forty three PKU pedigrees diagnosed at the First Affiliated Hospital of Zhengzhou University between 2019 and 2021 were selected as the study subjects. Variants of the PAH gene of the probands were screened by high-throughput sequencing, and candidate variants were verified by Sanger sequencing. Negative cases were further analyzed by multiplex ligation-dependent probe amplification (MLPA) to detect large fragment deletions and duplications of the PAH gene. For 43 women undergoing subsequent pregnancy, Sanger sequencing, MLPA, combined with short tandem repeats (STR) sequence-based linkage analysis, were carried out for prenatal diagnosis., Results: Among the 86 alleles carried by the 43 probands, 78 nucleotide variants (90.70%) and 3 large deletions (3.49%) were found based on high-throughput sequencing and MLPA. The 81 mutant alleles had included 21 missense variants, 5 splice site variants, 4 nonsense variants, 2 microdeletions, 1 insertional variant and 2 large fragment deletions. Relatively common variants have included p.Arg243Gln (23.26%), p.Arg111Ter (8.14%), EX6-96A>G (6.98%), p.Val399Val (5.81%) and p.Arg413Pro (4.65%). Most of the variants were located in exons 7, 11, 3, 6 and 12. For the 43 families undergoing prenatal diagnosis, 9 fetuses (20.45%) were diagnosed with PKU, 20 (45.45%) were heterozygous carriers, and 15 (34.09%) did not carry the same pathogenic allele as the proband. All neonates were followed up till 6 months old, and the accuracy of prenatal diagnosis was 100%., Conclusion: The combination of high-throughput sequencing, Sanger sequencing, MLPA and linkage analysis can increase the diagnostic rate of PKU and attain accurate prenatal diagnosis.

Objective: To investigate the clinical significance of TP53 allelic state in patients with myelodysplastic syndromes (MDS)., Methods: The clinical data of 858 MDS patients who underwent second-generation sequencing (NGS) testing in the First Affiliated Hospital of Soochow University from January 2019 to December 2021 were retrospectively analyzed., Results: The median age of the patients was 52 years old, and median follow-up time was 23.8 (0.4-109.6) months. Four hundred and one patients (46.7%) had at least one chromosomal abnormality, including 106 complex karyotypes and 78 monosomal karyotypes. A total of 103 cases of TP53 mutations were identified, with a mutation rate of 12%. Compared with TP53 wild-type, various types of chromosomal abnormalities were significantly more common in patients with TP53 mutations (all P < 0.001). Patients with TP53 mutations had lower hemoglobin levels, lower platelet counts and higher percentage of bone marrow primitive cell compared with TP53 wild type (all P < 0.05), and significantly shorter overall survival (OS). Among 97 evaluable patients, 33 cases (34%) were mono-allelic TP53 mutation, while 64 cases were bi-allelic TP53 mutation. Patients in bi-allelic TP53 mutation subgroup had a higher proportion of chromosomal abnormalities and a lower number of co-mutations compared with mono-allelic TP53 mutation. The median OS was 33.6 months in patients with mono-allelic state and only 11.4 months in patients with bi-allelic state ( HR =2.138, 95% CI : 1.053-4.343, P >0.05). Median OS was not reached in TP53 wild-type patients, and there was a significant difference in OS among TP53 wild-type, mono-allelic and bi-allelic TP53 mutation patients ( P < 0.001). Multivariable Cox regression analysis showed that bi-allelic TP53 was an independent predictor of poor outcomes ( HR =2.808, 95% CI : 1.487-5.003, P =0.001), while mono-allelic TP53 mutation and wild-type TP53 were not., Conclusion: Patients with TP53 mutations have a poor prognosis, and bi-allelic TP53 mutations have a worse prognosis compared with mono-allelic TP53 mutations and independently affect the prognosis of MDS patients.

Copyright of Journal of Henan Agricultural Sciences is the property of Editorial Board of Journal of Henan Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

[Objective] The purpose of this paper was to explore the genetic diversity of the tea variety ’Zijuan’ and its mutagenesis progeny.[Method] The selected ’Zijuan’ and ’Zijuan’ mutated progeny were investigated from several aspects of botanical characters, biochemical components and EST-SSR markers. [Result] It was found that the color of buds and leaves of 15 materials were similar to that of ’Zijuan’,only two materials in the leaf shape of mature leaves that were the same as ’Zijuan’,which was lanceolate, and the others were elliptic or long elliptic. There were 9 materials with the same leaf color as ’Zijuan’,all of which are purple green, while 7 materials were yellow-green. Among the variation coefficients of biochemical components in spring and summer, the highest variation coefficient was anthocyanin, which reached 72.16% in spring and 77.86% in summer. The content of anthocyanin changed more in summer than in spring. A total of 165 alleles were detected by microsatellite markers, the average number of alleles was 7.5,the effective number of alleles was 2.562,Shannon information index was 0.997,polymorphic information content was 0.492,observed heterozygosity was 0.629,and expected heterozygosity was 0.565. [Conclusion] The above analysis results of traits, physiological and biochemical characteristics and microsatellite markers showed that the mutant progeny of ’Zijuan’ had a relatively rich genetic diversity. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Henan Agricultural Sciences is the property of Editorial Board of Journal of Henan Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Henan Agricultural Sciences is the property of Editorial Board of Journal of Henan Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: High resolution validation of human leukocyte antigen (HLA) is one of the essential step before hematopoietic stem cell transplantation. With the continuous development of the work and the number of people tested continues to increase, human leukocyte antigen new alleles are constantly emerging. Due to the late discovery of some new genes and the lack of relevant reports, the gene frequency cannot be accurately calculated. In the absence of relevant data, it is easy to misjudge the typing results. The result of mismatch affects the success rate of transplantation. OBJECTIVE: An allele of HLA-A*24:353 was detected and confirmed in HLA, and its occurrence frequency was analyzed. METHODS: Polymerase chain reaction-sequencing-based typing method was used to detect five loci of HLA- A, B, C, DRB1, and DQB1 in 543 clinical transplantation matching recipients from September 2019 to May 2020. Next-generation sequencing was used to determine the full-length sequence of HLA-A*24:02/24:353 ambiguous results to obtain high resolution typing results of HLA and calculate the occurrence frequency of HLA-a *24:353 gene. RESULTS AND CONCLUSION: The ambiguous results of HLA-A*24:02/24:353 were detected in 146 cases. After further confirmatory tests using next-generation sequencing, the results of HLA-A*24:353 gene were detected in 5 cases (3.42%) in A*24 group and 0.92% in 543 cases. The results suggest that HLA-A*24:353 may not be rare allele in China. When HLA-A*24:02/24:353 ambiguous results appear in clinical transplantation matching recipients for high resolution typing, further confirmation is needed to increase the accuracy of typing results and improve the success rate of transplantation matching. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Henan Agricultural Sciences is the property of Editorial Board of Journal of Henan Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Contemporary Pediatrics is the property of Xiangya Medical Periodical Press and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Oil Crop Sciences is the property of Oil Crops Research Institute of Chinese Academy of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Modern Laboratory Medicine is the property of Journal of Modern Laboratory Medicine Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

[Objectives] In this study, we aimed to understand the sequence characteristics of the 3' -UTR of BMP7 gene in Suhuai pigs, and the relationship between its polymorphisms and reproductive traits, and 3 '-UTR activity, in order to provide reference for investigating the regulatory mechanism of the 3'-UTR of the porcine BMP7 gene. [Methods] The 3'-UTR sequence of Suhuai pig BMP7 gene was obtained by PCR amplification and direct sequencing, and its sequence characteristics were analyzed by bioinformatic methods. Mutations of the 3' -UTR of BMP7 gene in Suhuai pigs were screened by pooled-DNA sequencing, and then genotyped by direct sequencing. Association between polymorphisms and reproductive traits was analyzed by linear model. BMP7 3 '-UTR reporter vectors with different alleles were constructed, and luciferase assays were performed by using a double luciferase repmier gene system. [Results] A 639 bp of 3 '-UTR sequence of BMP7 gene was obtained from Suhuai pigs, which had high homology with other pig breeds, and contained classical cis-acting elements such as AU-rich element (ARE) and miRNA response element ( MRE). A mutation c. * 273A>G was found at 273 nt of the 3' -UTR sequence of BMP7 gene in Suhuai pigs. There were three genotypes in Suhuai pig population, among which AG was the dominant genotype and A was the dominant allele. Association analysis showed that the polymorphisms was significantly associated with the total number of piglets horn(TNB)of first parity,in which the sows with genotype AA had 0.83 piglets per litter more than the sows with genotype GG. Furthermore, the luciferase activity of reporter vector of the 3' -UTR with allele A was higher than that of allele G, but the difference was not significant. [Conclusions] BMP7 was a candidate gene for reproductive traits in Suhuai pigs. [ABSTRACT FROM AUTHOR]