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Objective To assess the presence of enteroviruses and diarrheal viruses in the environmental sewage of Aba in 2022, and to provide evidence supporting effective control measures against these viruses. Methods Sewage samples were collected monthly throughout 2022, then eluted and concentrated by anionic membrane adsorption. Through this, enteroviruses were isolated and identified. At the same time, nucleic acid was extracted from the concentrated liquid, and real-time fluorescent PCR was used to detect several diarrhea viruses, such as group A rotavirus, norovirus, Sapovirus, human astrovirus and enteric adenovirus. Results A total of 12 environmental sewage samples were collected from January to December 2022. Out of these, 3 strains of polioviruses and 9 non-polio enteroviruses were isolated. All 3 poliovirus strains were detected as Type 3 vaccine-like poliovirus (PV) strains. Group A rotavirus and norovirus genotype II were detected every month. Norovirus type I and enteric adenovirus were observed in all months except November. Human astrovirus was absent in samples from July, August, November and December. Sapovirus was not identified throughout the year. Conclusion Enteroviruses and diarrheal viruses exist widely in Aba's environmental sewage. Environmental sewage surveillance can offer a comprehensive view of these viruses' etiological and epidemiological characteristics. Continuous environmental sewage surveillance can predict and warn the epidemic and outbreak of enteroviruses and diarrheal virus-related diseases. [ABSTRACT FROM AUTHOR]

In order to solve the problem of early field inspection of porcine epidemic diarrhea, the experiment established a colloidal gold immunochromatographic method for the detection of porcine epidemic diarrhea virus (PEDV) in pig feces using a new kind of colloidal golds prepared via 3-hydroxymethyl aminomethane (Tris) assisted reduction. The preparation conditions including labeled pH and protein concentration was optimized. The sensitivity, specificity and accuracy of the established method were finally evaluated. The results showed that the established PEDV immunochromatography method could detect PEDV in pig feces within 15 min, and the minimum detection limit was 1×102 TCID50/mL. No cross reaction with porcine infectious gastroenteritis virus, rotavirus, Salmonella and other common pathogens was observed. In addition, there was no significant difference between intra-batch and inter-batch detection results. The coincidence rate of 107 clinical samples and RT-PCR was 100%. The study indicates that the established PEDV immunochromatographic strip based on the new colloidal gold marker had high sensitivity, strong specificity, good repeatability, with simple and quick operation, and is suitable for the rapid diagnosis of PED in primary farms. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Contemporary Pediatrics is the property of Xiangya Medical Periodical Press and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objectives To identify VP7, VP4 and NSP4 genotypes and analyze their sequence characterization of group A rotavirus (RVA) strains prevalent in Tianjin. Methods The fecal samples of patients suffered from RVA-positive cases of acute diarrhea in Tianjin during 2018-2020 were collected and viral RNA were extracted. VP7, VP4 and NSP4 gene fragments were amplified by RT-PCR and PCR products were sequenced to analysis phylogenetic tree. Results The genotyping results showed that all RVA prevalent in Tianjing (TJ) strains in this study were G9P[8]. Phylogenetic analysis confirmed that VP7 and VP4 belonged to lineages G9-VI and P[8]-3. Phylogenetic analysis of NSP4 confirmed that 19 RVAs belonged to E1 and 12 RVAs belonged to E2. Comparison with vaccine strains and international epidemic strains showed VP7, VP4 and NSP4 genes of TJ strains had amino acid substitution at key sites. Thirty-one TJ-VP7 amino acid sequences were found with 4 and 3 substitution sites each compared to Rotavac G9 and Rotasiil G9. Six and three amino acid differences were found for thirty-one TJ-VP8* nucleotide sequences each compared with Rotarix P[8]-1 and RotaTeq. Compared with the two vaccines, only one amino acid site variation of TJ-VP5* was found in I388L of 5-1 region of RotaTeq, and the other 11 amino acid sites were highly conserved, while the amino acid sequence of the Rotarix 5-1 to 5-5 region is identical. Compared with the vaccine strain, TJ-E1 strain had 2 and 3 amino acid mutations in ASI and ASII regions, respectively; TJ E2 strain had 3, 8, 2, and 5 amino acid substitutions in ASI-ASIV, respectively, compared to the NSP4 sequence of the vaccine strain. 19 G9P[8]-E1 and 12 G9P[8]-E2 were detected in Tianjin from 2018 to 2020, which is the first time a G9P[8]-E2 reassortant (IGR) strains was found in Tianjin. Conclution Compared to the same genotypic vaccines strains and epidemic strains, the effect of amino acids variations located in VP7,VP4 and NSP4 epitope regions of TJ strains on the protective efficacy of the vaccine needs to be further investigated. Continued monitoring of rotavirus molecular mutation and recombination phenomena is needed, not only for G\P typing, but also for consideration of multi-gene fragment co-analysis and whole genome sequencing, in order to grasp the recombination and recombination of rotavirus. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Henan Agricultural Sciences is the property of Editorial Board of Journal of Henan Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Rotavirus is the leading cause of severe diarrhea in children worldwide. To study the spread of rotavirus, a rotavirus transmission model was proposed based on the characteristics of temporary immunity after infection and maternal antibody protecting the newborn. By means of dynamic analysis, the basic reproduction number deciding the persistence of the infection was obtained. Based on the local stability analysis of the feasible equilibria, it was proved that the diseasefree equilibrium will be globally asymptotically stable if the basic reproduction number is no more than 1, through construction of appropriate Lyapunov functions. The disease will persist in the population if the basic reproduction number is more than 1 according to the Fonda lemma. [ABSTRACT FROM AUTHOR]

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Modern Laboratory Medicine is the property of Journal of Modern Laboratory Medicine Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)