Search

The present study has compared the anti-inflammatory activity of aqueous (OB W ) and methanolic (OB M ) extracts of aerial parts of Ocimum basilicum in macrophage (RAW264.7) and human chondrosarcoma (SW1353) cell lines, and human primary chondrocytes to correlate their efficacy in terms of management of osteoarthritis (OA). In RAW264.7, OB W decreased nitric oxide (NO) (35% ± 0.22) and prostaglandin (PGE 2 ) (70.8% ± 0.93) production more effectively compared with OB M . Interestingly, decrease in NO was accompanied by a corresponding decrease in inducible nitric oxide synthase (iNOS) (71.4 ± 2.43%) protein expression. OB W decreased total nuclear factor-kappa B (NFκB) (79.28% ± 1.8) and cyclooxygenase (COX)-2 (83.87% ± 0.95) proteins significantly ( p < 0.001), compared with OB M . Similarly, in SW1353 and chondrocytes, OB W decreased PGE 2 (76.11% ± 5.5) and leukotriene (LTB4) (59.6% ± 0.22) production appreciably ( p < 0.001), compared with OB M . In chondrocytes, OB W reduced the production of matrix metalloproteinase (MMP)-2 (58.49% ± 1.41), −9 (43.13% ± 2.82) and −13 (54.54% ± 2.12) significantly more ( p < 0.001), than OB M. All these data suggest that compared with the methanolic extract, the aqueous extract of O. basilicum could be explored for its potential applications in the management of inflammatory conditions associated with OA. [ABSTRACT FROM AUTHOR]

• UHRF1 is upregulated in several cancers. • UHRF1 levels are associated with stage, size and grade of the tumor. • UHRF1 can be used as a druggable target. • Docking of Propranolol, Nocodazole, Thymoquinone, Emodin, Napthazarin to SRA domain. The figure describes the location of UHRF1 (Ubiquitin-like with containing PHD and RING Finger domains 1) gene, mRNA and protein synthesis in the tumor cell and its structural domains with a focus on the docking of Naphthazarin on the SRA domain of UHRF1, resulting in reduction of tumor size. [ABSTRACT FROM AUTHOR]