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In this study, 37 derivatives of phorbol esters were synthesized and their anti-HIV-1 activities evaluated, building upon our previous synthesis of 51 phorbol derivatives. 12-Para-electron-acceptor- trans -cinnamoyl-13-decanoyl phorbol derivatives stood out, demonstrating remarkable anti-HIV-1 activities and inhibitory effects on syncytia formation. These derivatives exhibited a higher safety index compared with the positive control drug. Among them, 12-(trans -4-fluorocinnamoyl)-13-decanoyl phorbol, designated as compound 3c , exhibited the most potent anti-HIV-1 activity (EC 50 2.9 nmol·L−1, CC 50 /EC 50 11 117.24) and significantly inhibited the formation of syncytium (EC 50 7.0 nmol·L−1, CC 50 /EC 50 4891.43). Moreover, compound 3c is hypothesized to act both as an HIV-1 entry inhibitor and as an HIV-1 reverse transcriptase inhibitor. Isothermal titration calorimetry and molecular docking studies indicated that compound 3c may also function as a natural activator of protein kinase C (PKC). Therefore, compound 3c emerges as a potential candidate for developing new anti-HIV drugs. [ABSTRACT FROM AUTHOR]

Understanding the response of wetlands to climate changes could provide useful insights toward predicting the wetlands future in a warmer world. Here, we present a well-dated peat/mud profile with multiple proxies to reconstruct the historic development of Honghe wetland and discuss its response to the East Asian monsoon variations during the Holocene. The results show that the Honghe wetland developed as a shallow-water lake in the mid-early Holocene during a time of high precipitation from the East Asian monsoon. At 4600 years BP, peat layers appeared with sharp increases in the accumulation rate, grain-size Md and arboreal plants, marking a decline in water levels with the relative dry climate. Moreover, the transition corresponds well to the mid-Holocne monsoon weakening event and we suggest the decrease of the monsoon associated precipitation plays a critical role in driving the lake-peatland transition in Honghe. Facing the intensified summer monsoon in future, we suggest some practical measures should be taken to protect the present wetlands from changing back to lakes. [ABSTRACT FROM AUTHOR]

[Display omitted] • The bacterial variation within microhabitats associated with the black stone of the Black Gobi desert were investigated. • Bacterial communities within microhabitats were highly different in the desert ecosystems. • Microhabitats formed by black stones support highly diverse and biologically active bacterial communities. • Major environmental controls were water content, total carbon, and total nitrogen. Desert soil around the black stones is highly complex, which substantially affects the diversity and composition of inhabiting microbes. The existence of black stones in the southern part of the Black Gobi desert of China could provide microhabitats for diverse bacterial communities that remain unexplored. Hence, Illumina MiSeq sequencing was used to determine the differences in bacterial communities associated within microhabitats in three sites of the Black Gobi desert, China. Our results show that bacterial communities are significantly affected by each microhabitat. For instance, the α -diversity of bacterial communities indicated more remarkable diversity and richness in these microhabitats. Considering β -diversity, variances were reported mainly in the Proteobacteria (30%), Actinobacteria (26%), Chloroflexi (19%), and Firmicutes (9%). Firmicutes were markedly enriched in the upper surface, especially in site 1. Compared to other microhabitats, the relative abundance of Proteobacteria was greater in the subsurface, and they were also more dominant in the other two sites. Network analysis of soil factors and bacterial genera showed that the most significant-occurrences were positively correlated, demonstrating potential synergistic interactions. Collective with the predicted function profiles and the redundancy analysis, these results indicated the highest variances in bacterial community structure and function in Black Gobi Desert ecosystems. These differences are likely closely related to the soil parameters, mainly water content, total carbon, and total nitrogen, and might be associated with black stones. This study concludes that microhabitats formed by black stones support highly diverse and biologically active bacterial communities. These microhabitats with extreme environmental conditions deliver new opportunities to explore soil bacterial communities at relevant spatial scales in the Black Gobi desert. [ABSTRACT FROM AUTHOR]

Fucoidans are complex sulfated polysaccharides extracted from brown algae. Depending on the concentration, they have been shown to stimulate and inhibit blood coagulation in vitro . Promotion of coagulation is mediated by blocking tissue factor pathway inhibitor (TFPI). We screened fucoidan extracts from four brown algae species in vitro with respect to their potential to improve coagulation in bleeding disorders. The fucoidans’ pro- and anticoagulant activities were assessed by global hemostatic and standard clotting assays. Results showed that fucoidans improved coagulation parameters. Some fucoidans also activated the contact pathway of coagulation, an undesired property reported for sulfated glycosaminoglycans. Chemical evaluation of fucoidans’ complex and variable structure included molecular weight ( Mw ), polydispersity (polyD), structural heterogeneity, and organic and inorganic impurities. Herewith, we describe a screening strategy that facilitates the identification of crude fucoidan extracts with desired biological and structural properties for improvement of compromised coagulation like in hemophilia. [ABSTRACT FROM AUTHOR]

Compared to the well-studied the grain-size depositional process in hydraulic and aeolian sediments, the process in wetlands is still poorly understood. In this study, the grain-size components of a well-dated mud/peat profile (SJ2) in the Sanjiang Plain were partitioned using a lognormal distribution function. The results suggest that the lacustrine deposits contain four distinct unimodal grain-size distributions, C-1 through C-4, with their modal sizes ranging 0.7–1.6, 2.4–8.7, 15.7–27.8, and 380.2–529.6 μm respectively. Only the C-1 and C-3 were identified in the peat layers. A grain-size depositional modal was introduced to explain the environmental meanings of the four components. C-3, as a nearshore suspension component, was the most sensitive component to the variation of local hydraulic conditions. Based on the variation of C-3 percentages of the profile SJ2, the local hydraulic conditions were tentatively reconstructed, and the results showed a close relation with the monsoon variations during the Holocene. Accordingly, we suggest the relative coarse component of the polymodal peat deposits in monsoonal regions can be used as a sensitive indicator for the hydraulic conditions and the monsoon circulations in the geological past. [ABSTRACT FROM AUTHOR]

Abstract: The novel low-molecular-weight chitosan polysulfate (MW 5120–26,200Da) was prepared using the depolymerization of chitosan with papain (EC. 3.4.22.2). The sulfonation of depolymerized products was performed using chlorosulfonic acid in N,N-dimethylformamide under semi-heterogeneous conditions. The structures of the products were characterized by FTIR, 13C NMR, and 1H NMR (1D, 2D NMR) spectroscopy. The present study sheds light on the mechanism of anticoagulant activity of chitosan polysulfate. Anticoagulant activity was investigated by an activated partial thromboplastin assay, a thrombin time assay, a prothrombin time assay, and thrombelastography. Surface plasmon resonance also provided valuable data for understanding the relationship between the molecular binding of sulfated chitosan to two important blood clotting regulators, antithrombin III and heparin cofactor II. These results show that the principal mechanism by which this chitosan polysulfate exhibits anticoagulant activity is mediated through heparin cofactor II and is dependent on polysaccharide molecular weight. [Copyright &y& Elsevier]

Abstract: Propagation of faults and folds in the foreland basins of Tian Shan is an important process accommodating Cenozoic crustal shortening and mountain building, but little is known about the accurate time of the Cenozoic tectonic deformation. Based on growth strata and age determination, we show that syntectonic growth strata began to develop in the middle part of Tian Shan since 6 Ma ago. Geometry analysis indicates that formation of the growth strata is associated with progressive fold-limb rotation. Formation of the growth strata is contemporaneous with the tectonic deformation in the thrusting and folding zones. Together with the remarkable increase of sedimentation rate as well as the accumulation of coarse molasse deposits, we conclude that the late Cenozoic crustal shortening and mountain building in the region initiated since about 6 Ma and lasted to the early Pleistocene, as a consequence of intracontinental deformation within the India–Eurasia convergent system. [Copyright &y& Elsevier]

Alginate that was purified from the fermentation solution of marine bacteria Vibro sp. 510 under specific reaction conditions was hydrolyzed by alginate lyase. Seven oligosaccharides, including di-, tri- and tetrasaccharides, were isolated through low-pressure, gel-permeation chromatography (LP-GPC) and semipreparative strong-anion exchange (SAX) fast-protein liquid chromatography (FPLC). The oligosaccharide structures were elucidated based on ESIMS and 2D NMR spectral analysis. The hydrolytic specificity of this alginate lyase to alginate is discussed. [Copyright &y& Elsevier]

Schematic figure showing climate driving mechanisms on wetland hydrological and vegetational evolution. The relatively strong East Asian summer monsoon (EASM) with high precipitation tends to generate a higher water-level condition with more hygrophilous herbaceous plants while blocking the deposition of coarse grain-size fractions (a), and vise versa for weak EASM stages (b). [Display omitted] • The late-Holocene hydrological and vegetational regimes were reconstructed. • An ecological lake-to-wetland transition occurred at ~4.5 ka BP. • The wetlands evolution was largely controlled by monsoonal precipitation. The responding mechanism of wetland ecosystem to climate change is currently unclear due to a lack of long-term records incorporating multiple ecological factors. This paper presents a well-dated and multi-proxy cored record from a natural freshwater wetland developed in a paleo-pingo-related depression on the Sanjiang Plain. The study aims to reconstruct the wetland water-level and vegetational regimes during the past ~6.0 ka BP and discuss the wetland evolution response to the East Asian summer monsoon (EASM) circulation. The results show that a shallow-water lake with hygrophilous herbs developed in the depression with a strong EASM during ~6.0–4.5 ka BP. The paleolake was succeeded by a wetland with a lowered mean water level and an increase in tree cover controlled by the weakened EASM during the past 4.5 ka BP. Such an ecological lake-to-wetland transition corresponds well to the sharp decline of the EASM around 4.5 ka BP which has been well documented in various geological records across the EASM domain. During the wetland developing stage over the past 4.5 ka BP, the water-level and vegetational changes in the studied depression were also closely related to the EASM variations. The strengthened EASM usually generated higher water levels with more hygrophilous herbs around 4.0 ka BP, 3.5 ka BP, 3.0 ka BP, 2.0 ka BP, and 1.3 ka BP. Considering the prevalent EASM climate serving as the predominant water supply for the wetlands on the modern Sanjiang Plain, we suggest the EASM circulation has played a critical role in driving the wetland ecological evolution during the mid-to-late Holocene Epoch. The past 0.4 ka BP is an exception as the local Trees/Herbs ratio visibly increased with the declined water level, while the monsoonal precipitation shows no equivalent shift. The wetland evolution at this stage was attributed to human interference. [ABSTRACT FROM AUTHOR]

As a kind of Fabry-Perot cavities, one-dimensional photonic crystals (1DPCs) with defect layers have been widely utilized for narrow-band filtering. However, according to the Bragg interference condition, photonic band gaps (PBGs) in conventional all-dielectric 1DPCs shift towards shorter wavelengths (blueshift) as the incident angle increases. Therefore, transmittance peaks in all-dielectric one-dimensional defective PCs also shift along with PBGs as the incident angle increases. Recently, the emergence of angle-insensitive PBGs in 1DPCs containing hyperbolic metamaterials (HMMs) provides us a possibility to realize omnidirectional narrow-band filtering. In this paper, we design an angle-insensitive ultraviolet PBG in a 1DPC containing HMM, where HMM layer is mimicked by a subwavelength dielectric-metal multilayer. By introducing a defect layer into the 1DPC and adjusting the dielectric thickness within the 1DPC, we achieve omnidirectional narrow-band ultraviolet filtering with angle-insensitive property. This omnidirectional narrow-band filtering might possess applications in digital display, organic solar cell and wavelength division demultiplexing. [ABSTRACT FROM AUTHOR]

An integrated view of wetland's evolution is currently poorly understood due to a lack of knowledge on long-term interactions of multiple ecological factors. Here, we present a cored palynological record covering the Holocene Epoch from a depressional wetland on Heixiazi Island (China). With the aid of principal component analysis and cluster analysis of the palynological data, the historical vegetation regime has been well reconstructed for wetlands on the island. With further assistance from the published data on local hydrology and regional East Asian summer monsoon (EASM) variations, the interactions of vegetation, hydrology, and climate in the island's wetlands have been thoroughly analyzed with correlation analysis of the three factors. The results indicate that a strong EASM generally led to a high water level in the wetlands from increased monsoonal precipitation, causing an increase in arboreal vegetation and a decrease in herbs. Alternatively, a weak EASM generally led to low water levels due to decreased monsoonal precipitation, causing a decrease in arboreal vegetation and an increase in herbs. The local vegetation regime during the early Holocene was marked by an increase in tree/herb ratio due to rising water levels under the influence of an increasingly strengthened EASM. Subsequently, a general decline of the tree/herb ratio occurred from a gradual decrease in water level during the mid and late Holocene when the EASM gradually retreated. The wettest stage marked by the highest water level and tree/herb ratio occurred 8.0–4.6 ka BP with the strongest EASM. The driest stage occurred during the last 0.4 ka BP, which was attributed to both the weakest EASM influence and anthropogenic disturbance. Our study provides an integrated view of the wetlands' ecological dynamics incorporating multi-factor interactions, which further sheds light on the EASM driving mechanisms on wetlands evolution during the Holocene Epoch. Schematic figure showing the historical interactions of vegetation, hydrology, and climate in a typical wetland on Heixiazi Island, NE China. The relatively strong East Asian summer monsoon (EASM) with high atmospheric precipitation tends to generate a higher water level with less herbaceous plants (a), while a weaker EASM stage with less precipitation leads to a lower water level and more herbaceous plants (b). The light blue and gray arrows indicate the separate pollen contributions of arboreal trees in elevated areas and herbs within the depressional wetland, respectively. Unlabelled Image • A palynological methodology aided in reconstructing vegetation history. • Holocene climatological and hydrological regimes were determined. • Holocene interactions of vegetation, hydrology, and climate were presented. • Wetland evolution was largely controlled by monsoonal precipitation. [ABSTRACT FROM AUTHOR]

A novel homogenous polysaccharide LPWF together with its three acid hydrolysis products LPWF1–3 were isolated and prepared from lotus plumule (germs of Nelumbo nucifera). LPWF was composed of rhamnose (Rha), arabinose (Ara), galactose (Gal), xylose (Xyl), and galacturonic acid (GalA) in the molar ratio of 7.3: 34.0: 7.0: 19.1: 32.6 with a molecular weight of 567.6 kDa. The structure of LPWF was elucidated by methylation and NMR analysis of LPWF1–3 and a follow-up structural assembling aided by high-resolution mass spectrometry mapping of oligosaccharides and ROSEY spectra. LPWF was characterized as an unusual pectin linked by rhamnogalacturonan I (RGI, composed of LPWF1–2) and xylogalacturonan (XGA, LPWF3). LPWF1 was an arabinan peeled from the RGI part with a 1,5-linked backbone branching on the O -2 position, while LPWF2 was the remaining part of RGI composed of Rha (36.1%), Gal (17.8%), and GalA (43.7%). LPWF3 was identified as the XGA part with a backbone of α-1,4-linked GalA and branches of mono-xylose substitutions on the O -3 of GalA. LPWF (25 μg/mL) demonstrated significant inhibitions on the expression of IL-1β, IL-6, and TNF-α in LPS-stimulated primary murine microglia cultures. LPWF1 and 2 showed selectively and significantly inhibitory activity against the expression of IL-1β. • Structure features of the pectin LPWF was determined by NMR and methylation. • Structural characterization of LPWF was elucidated by assembling three stepwise hydrolyzed fragments. • LPWF is speculated to be a linearly linked RGI and XGA pectin. • The pectin LPWF could significantly inhibit the expression of IL-1β, IL-6, and TNF-α. [ABSTRACT FROM AUTHOR]

Heparin, an old but first-line anticoagulant, has been used over a century. It is a heterogeneous, linear, highly sulfated, anionic glycosaminoglycan with a broad distribution in relative molecular weight and charge density. These structural properties allow heparin to selectively interact with multiple proteins, leading to heparin's various pharmacological functions, such as anticoagulant, anti-viral, anti-tumor and anti-inflammatory activities. Clinical data suggest that unfractionated heparin or low molecule weight heparin could decrease mortality in COVID-19 patients with sepsis-induced hypercoagulation through the anticoagulant, anti-viral and anti-inflammatory activities of these drugs. Thus, the non-anticoagulant activity of heparin has again aroused attention. This review highlights recent advances in the preparation of heparin-derived drugs and clinical research on its non-anticoagulant properties over the past decade, to further the development and utilization of these important drugs. [Display omitted] [ABSTRACT FROM AUTHOR]

Abstract: The sulfo groups of glycosaminoglycans contribute to their high charge densities, and are critical for the role they play in various physiological and pathophysiological processes. Unfortunately, the sulfo groups can be hydrolyzed to inorganic sulfate. Thus, it is important to monitor the presence of these sulfo groups. In addition, free anions, including chloride, sulfate and acetate, are often present in glycosaminoglycans as a result of multiple purification steps, and their presence also needs to be monitored. In this report, ion chromatography with conductivity detection is used to analyze the anions present in glycosaminoglycans, including heparin, heparan sulfate, chondroitin sulfate and dermatan sulfate. This method allows quantitation over a wide range of concentrations, affording a limit of quantitation of 0.1ppm and a limit of detection of 0.05ppm for most anions of interest. The stability of heparin was also studied, providing data on the formation of both sulfate and acetate anions. [Copyright &y& Elsevier]