1. Gambogic acid induces growth inhibition and differentiation via upregulation of p21waf1/cip1 expression in acute myeloid leukemia cells.
- Author
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Chen, Yan, Hui, Hui, Li, Zheng, Wang, Hong-Mei, You, Qi-Dong, and Lu, Na
- Subjects
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ANALYSIS of variance , *ANTINEOPLASTIC agents , *BIOLOGICAL assay , *CARBOXYLIC acids , *CELL culture , *CELL differentiation , *CELL physiology , *FLOW cytometry , *GENE expression , *GUMS & resins , *MOLECULAR structure , *RESEARCH funding , *TOXICITY testing , *WESTERN immunoblotting , *ACUTE myeloid leukemia , *DATA analysis software , *DESCRIPTIVE statistics - Abstract
Gambogic acid (GA) is the major active ingredient of gamboges, a brownish to orange resin product from Garcinia hanburyi tree in Southeast Asia. This compound exhibits anti-cancer effect on solid tumors. In this study, we investigated the effects of GA on the growth and differentiation of acute myeloid leukemia cells by growth-inhibition detection, morphological changes observation, nitroblue tetrazolium reduction, and the expression of the relative cell-surface differentiation markers. The results showed that GA could inhibit cell growth and promote differentiation in U937 and HL-60 cells. In addition, GA upregulated the expression of p21waf1/cip1 in the two cell lines. Finally, downregulating the p21waf1/cip1 expression with small interfering RNA partially blocked GA-induced cell growth inhibition and differentiation. These results of this study revealed that GA may be used as one of the investigational drugs for acute myeloid leukemia. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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