65 results on '"Fleming, Andrew"'
Search Results
2. Cellular perspectives for improving mesophyll conductance.
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Lundgren, Marjorie R. and Fleming, Andrew J.
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LEAF physiology , *GAS flow , *RATE of return , *CROP yields , *SYSTEMS engineering , *FLUX (Energy) - Abstract
Summary: After entering the leaf, CO2 faces an intricate pathway to the site of photosynthetic fixation embedded within the chloroplasts. The efficiency of CO2 flux is hindered by a number of structural and biochemical barriers which, together, define the ease of flow of the gas within the leaf, termed mesophyll conductance. Previous authors have identified the key elements of this pathway, raising the prospect of engineering the system to improve CO2 flux and, thus, to increase leaf photosynthetic efficiency. In this review, we provide a perspective on the potential for improving the individual elements that contribute to this complex parameter. We lay particular emphasis on generation of the cellular architecture of the leaf which sets the initial boundaries of a number of mesophyll conductance parameters, incorporating an overview of the molecular transport processes which have been proposed as major facilitators of CO2 flux across structural boundaries along the pathway. The review highlights the research areas where future effort might be invested to increase our fundamental understanding of mesophyll conductance and leaf function and, consequently, to enable translation of these findings to improve the efficiency of crop photosynthesis. Significance Statement: Increasing CO2 flux has been identified as an element of leaf structure/function which could significantly improve photosynthetic efficiency and, thus, crop yield. This review highlights the challenges and opportunities of achieving this aim, bringing into focus our ignorance of some fundamental aspects of leaf developmental physiology, and identifying areas where future research investment could yield improved knowledge, understanding, and impact. [ABSTRACT FROM AUTHOR]
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- 2020
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3. The developmental relationship between stomata and mesophyll airspace.
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Baillie, Alice L. and Fleming, Andrew J.
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WATER efficiency , *MESOPHYLL tissue , *LEAF development , *CROP improvement , *GAS exchange in plants , *STOMATA , *AGROHYDROLOGY - Abstract
Summary: The quantitative and spatial coordination of stomatal pores in the epidermis and airspaces in the underlying mesophyll tissue is vital for efficient gas exchange in the leaf. The mechanisms that determine the distribution of stomata in the epidermis have been studied extensively, but how this relates to the regulation of mesophyll airspace configuration is poorly understood. Recent studies have investigated how development is coordinated between these tissue layers. The evidence suggests that multiple mechanisms are likely to work concurrently to coordinate stomatal and mesophyll development for optimal leaf gas exchange, and that both genetic and physiological factors contribute to this regulation. Such advances in our understanding of leaf development have important implications for potential improvement of crop water use efficiency. [ABSTRACT FROM AUTHOR]
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- 2020
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4. Resonance-Enhanced Coupling for Range Extension of Electromagnetic Tracking Systems.
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Islam, Mohd Noor and Fleming, Andrew J.
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ELECTROMAGNETIC coupling , *MAGNETIC sensors , *DEGREES of freedom , *BANDWIDTHS , *SURFACE discharges (Electricity) , *MAGNETIC resonance - Abstract
This paper investigates the use of resonance-enhanced coupling to increase the received signal level in a six-degree-of-freedom electromagnetic tracking system. Resonant coupling is found to increase the efficiency of the transmitter and increase the gain of the sensing coil, resulting in improved range. However, the measurement update rate is reduced due to the settling time of the transmitter circuit and the limited bandwidth of the sensing circuit. A resistive tuning approach is proposed to balance the tradeoff between a decreased measurement bandwidth and an improved signal level. [ABSTRACT FROM AUTHOR]
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- 2018
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5. An Algorithm for Transmitter Optimization in Electromagnetic Tracking Systems.
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Islam, Mohd Noor and Fleming, Andrew J.
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COILS (Magnetism) , *TRACKING control systems , *COMPRESSED sensing , *MAGNETIC fields , *ALGORITHMS (Physics) - Abstract
Electromagnetic tracking systems are used extensively in biomedical devices, gaming consoles, and animation because they are inexpensive and do not require line of sight. However, the measurement range is limited by the amplitude of the induced voltage in the sensing coil. The induced voltage is a function of the transmitter parameters such as the coil dimensions, signal power, and frequency. The transmitted power is typically restricted by the physical constraints in the application. This paper develops an algorithm to optimize the dimensions of the transmitting coil for maximum induced voltage. The proposed method is suitable for a transmitter consisting of three concentric orthogonal transmitting coils of the type commonly used in six-degree-of-freedom localization. The simulation and experimental results are presented, which demonstrate the efficacy of the proposed method. [ABSTRACT FROM PUBLISHER]
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- 2017
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6. Dissecting Hobby Lobby's Corporate Person.
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Fleming, Andrew J.
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BURWELL v. Hobby Lobby Stores Inc. , *CITIZENS United v. Federal Election Commission , *DUE process of law ,RELIGIOUS Freedom Restoration Act of 1993 (U.S.) - Abstract
The article reports the decision of the U.S. Supreme court in the case 'Burwell v. Hobby Lobby Stores Inc.' in which the Court held that for-profit corporations are "persons" under the Religious Freedom Restoration Act (RFRA). Topics discussed include the U.S. Supreme Court's decision in the case 'Citizens United v. FEC; ' expansion of First Amendment political speech rights to corporations; and adoption of the Fourteenth Amendment to the U.S. Constitution.
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- 2016
7. Improving Digital-to-Analog Converter Linearity by Large High-Frequency Dithering.
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Eielsen, Arnfinn A. and Fleming, Andrew J.
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DIGITAL-to-analog converters , *HARMONIC distortion (Physics) , *DISTRIBUTION (Probability theory) - Abstract
A new method for reducing harmonic distortion due to element mismatch in digital-to-analog converters is described. This is achieved by using a large high-frequency periodic dither. The reduction in nonlinearity is due to the smoothing effect this dither has on the nonlinearity, which is only dependent on the amplitude distribution function of the dither. Since the high-frequency dither is unwanted in the output of the digital-to-analog converter, the dither is removed by an output filter. The fundamental frequency component of the dither is attenuated by a passive notch filter and the remaining fundamental component and harmonic components are attenuated by the low-pass reconstruction filter. Two methods that further improve performance are also presented. By reproducing the dither on a second channel and subtracting it using a differential amplifier, additional dither attenuation is achieved; and by averaging several channels, the noise-floor of the output is improved. Experimental results demonstrate more than 10 dB improvement in the signal-to-noise-and-distortion ratio. [ABSTRACT FROM AUTHOR]
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- 2017
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8. On the Atmospheric Correction of Antarctic Airborne Hyperspectral Data.
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Black, Martin, Fleming, Andrew, Riley, Teal, Ferrier, Graham, Fretwell, Peter, McFee, John, Achal, Stephen, and Diaz, Alejandra Umana
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RADIATIVE transfer , *STANDARD deviations , *ATMOSPHERIC aerosols , *CALIBRATION , *HYPERSPECTRAL imaging systems - Abstract
The first airborne hyperspectral campaign in the Antarctic Peninsula region was carried out by the British Antarctic Survey and partners in February 2011. This paper presents an insight into the applicability of currently available radiative transfer modelling and atmospheric correction techniques for processing airborne hyperspectral data in this unique coastal Antarctic environment. Results from the Atmospheric and Topographic Correction version 4 (ATCOR-4) package reveal absolute reflectance values somewhat in line with laboratory measured spectra, with Root Mean Square Error (RMSE) values of 5% in the visible near infrared (0.4-1 µm) and 8% in the shortwave infrared (1-2.5 µm). Residual noise remains present due to the absorption by atmospheric gases and aerosols, but certain parts of the spectrum match laboratory measured features very well. This study demonstrates that commercially available packages for carrying out atmospheric correction are capable of correcting airborne hyperspectral data in the challenging environment present in Antarctica. However, it is anticipated that future results from atmospheric correction could be improved by measuring in situ atmospheric data to generate atmospheric profiles and aerosol models, or with the use of multiple ground targets for calibration and validation. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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9. On the Atmospheric Correction of Antarctic Airborne Hyperspectral Data.
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Black, Martin, Fleming, Andrew, Riley, Teal, Ferrier, Graham, Fretwell, Peter, McFee, John, Achal, Stephen, and Umana Diaz, Alejandra
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RADIATIVE transfer , *STANDARD deviations , *AEROSOLS , *PENINSULAS - Abstract
The first airborne hyperspectral campaign in the Antarctic Peninsula region was carried out by the British Antarctic Survey and partners in February 2011. This paper presents an insight into the applicability of currently available radiative transfer modelling and atmospheric correction techniques for processing airborne hyperspectral data in this unique coastal Antarctic environment. Results from the Atmospheric and Topographic Correction version 4 (ATCOR-4) package reveal absolute reflectance values somewhat in line with laboratory measured spectra, with Root Mean Square Error (RMSE) values of 5% in the visible near infrared (0.4-1 μm) and 8% in the shortwave infrared (1-2.5 μm). Residual noise remains present due to the absorption by atmospheric gases and aerosols, but certain parts of the spectrum match laboratory measured features very well. This study demonstrates that commercially available packages for carrying out atmospheric correction are capable of correcting airborne hyperspectral data in the challenging environment present in Antarctica. However, it is anticipated that future results from atmospheric correction could be improved by measuring in situ atmospheric data to generate atmospheric profiles and aerosol models, or with the use of multiple ground targets for calibration and validation. [ABSTRACT FROM AUTHOR]
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- 2014
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10. The co-ordination of cell division, differentiation and morphogenesis in the shoot apical meristem: a perspective.
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Fleming, Andrew J.
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CELL proliferation , *REJUVENESCENCE (Botany) , *CELL division , *PLANT cells & tissues , *MORPHOGENESIS - Abstract
Whether morphogenesis is cell division-driven or organismal-based has been a long-running debate in plant biology. This article is a summary of a series of experiments aimed at distinguishing these alternate views by local manipulation of parameters of cell division frequency, orientation, and growth within the shoot apical meristem. These data, put in the context of other investigations in this area, support an organismal view of plant morphogenesis and support the idea that the cell wall plays a key role in the mechanism by which this is achieved. At the same time, the data indicate that the intimate but variable relationship between cell growth and division within the organism means that cell proliferation can indirectly influence this process, leading to a context-dependent influence on morphogenesis. Finally, cell growth and proliferation are intimately related with the process of differentiation as cells exit the meristem. In the final part of the article the molecular mechanism by which these basic cellular parameters are intertwined is discussed. [ABSTRACT FROM PUBLISHER]
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- 2006
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11. The control of leaf development.
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Fleming, Andrew J.
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LEAVES , *MERISTEMS , *PLANT cells & tissues , *PLANT growth , *PATTERN formation (Biology) - Abstract
Summary 1I. Introduction 1II. The shoot apical meristem: the creation of a field of cells from which leaves can be formed 2III. Patterning: the selection of leaf initiation site 2IV. Morphogenesis: the initial stages of leaf formation 4V. Differentiation and determination: transcriptional networks controlling determinancy 6VI. The elaboration of leaf form 6VII. Early steps in leaf histogenesis 7VIII. Later steps in leaf differentiation: epidermal cell fate 8IX. Compound and simple leaves: variation on a theme? 9X. Changes in leaf form: developmental and environmental influences 9XI. A model of leaf formation 9 Acknowledgements 10 References 10The formation of a leaf is a basic aspect of plant development. This review provides an overview of our present understanding of the process from initiation to the final form of the leaf. Molecular genetic and cell biology approaches have yielded significant advances in this area, adding not only to our knowledge of leaf development but also to fundamental principles in plant biology. These principles will be highlighted, as well as areas where our understanding is still incomplete, in particular the problem of coordinating the multifaceted steps involved in the generation of the leaf structure.New Phytologist(2004)doi: 10.1111/j.1469-8137.2004.01292.x© New Phytologist(2004) [ABSTRACT FROM AUTHOR]
- Published
- 2005
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12. Cell division pattern influences gene expression in the shoot apical meristem.
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Wyrzykowska, Joanna and Fleming, Andrew
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CELL division , *GENE expression , *MERISTEMS , *ANGIOSPERMS - Abstract
The shoot apical meristem of angiosperms shows a highly conserved cellular architecture in which a change of cell division orientation correlates with early events of leaf initiation. However, the causal role of this altered cellular parameter in leaf formation is debatable. We have used the dynamin-like protein phragmopiastin as a tool to modify the pattern of cell division within the apical meristem. Taking a microinduction approach, we show that local alteration in cell division orientation is not sufficient to induce morphogenesis in the meristem. Surprisingly, an altered cell division pattern did lead to an altered pattern of expression of genes implicated in aspects of leaf formation. Our data identify inducible expression of phragmoplastin as a tool to manipulate cell division pattern. Furthermore, they indicate that a mechanism exists by which cells in the meristem can respond at the level of gene expression to altered parameters of cell division. These data are discussed in the context of a model linking leaf morphogenesis and differentiation. [ABSTRACT FROM AUTHOR]
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- 2003
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13. Induction of leaf primordia by the cell wall protein expansin.
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Fleming, Andrew J. and McQueen-Mason, Simon
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PLANT cell walls , *EXTRACELLULAR matrix proteins , *PHYSIOLOGY , *CHEMICAL structure - Abstract
Informs about the role that expansin, an extracellular protein, plays in the increasing of plant cell wall extensibility. Expansin inducing tissue expansion in vivo; Localized control of tissue expansion possibly sufficient to induce leaf formation; Suggestion of a role for biophysical forces in the regulation of plant development.
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- 1997
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14. Producing patterns in plants.
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Fleming, Andrew J.
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PLANT morphogenesis , *PLANT morphology , *FLOWERS , *INFLORESCENCES , *AUXIN , *PLANT hormones - Abstract
The article provides a quantitative analysis of the process of capitulum growth and the gradual but controlled process by which elements within the capitulum become destined to form individual flowers. The data provide a foundation for the modelling of flower formation and for linking the observed parameters of floral morphogenesis with the wealth of molecular data on organogenesis in plants. An analysis of auxin flux and distribution during leaf initiation is also presented. The situation in the capitulum may be linked with the spatial and temporal control of factors specific to flower formation.
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- 2006
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15. Synthesis and import of GDP‐l‐fucose into the Golgi affect plant–water relations.
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Waszczak, Cezary, Yarmolinsky, Dmitry, Leal Gavarrón, Marina, Vahisalu, Triin, Sierla, Maija, Zamora, Olena, Carter, Ross, Puukko, Tuomas, Sipari, Nina, Lamminmäki, Airi, Durner, Jörg, Ernst, Dieter, Winkler, J. Barbro, Paulin, Lars, Auvinen, Petri, Fleming, Andrew J., Andersson, Mats X., Kollist, Hannes, and Kangasjärvi, Jaakko
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GOLGI apparatus , *FUCOSE , *FUCOSYLATION , *ARABIDOPSIS thaliana , *IMPORTS , *WATER efficiency - Abstract
Summary: Land plants evolved multiple adaptations to restrict transpiration. However, the underlying molecular mechanisms are not sufficiently understood.We used an ozone‐sensitivity forward genetics approach to identify Arabidopsis thaliana mutants impaired in gas exchange regulation.High water loss from detached leaves and impaired decrease of leaf conductance in response to multiple stomata‐closing stimuli were identified in a mutant of MURUS1 (MUR1), an enzyme required for GDP‐l‐fucose biosynthesis. High water loss observed in mur1 was independent from stomatal movements and instead could be linked to metabolic defects. Plants defective in import of GDP‐l‐Fuc into the Golgi apparatus phenocopied the high water loss of mur1 mutants, linking this phenotype to Golgi‐localized fucosylation events. However, impaired fucosylation of xyloglucan, N‐linked glycans, and arabinogalactan proteins did not explain the aberrant water loss of mur1 mutants.Partial reversion of mur1 water loss phenotype by borate supplementation and high water loss observed in boron uptake mutants link mur1 gas exchange phenotypes to pleiotropic consequences of l‐fucose and boron deficiency, which in turn affect mechanical and morphological properties of stomatal complexes and whole‐plant physiology. Our work emphasizes the impact of fucose metabolism and boron uptake on plant–water relations. [ABSTRACT FROM AUTHOR]
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- 2024
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16. Pathogenic Variant Frequencies in Hereditary Haemorrhagic Telangiectasia Support Clinical Evidence of Protection from Myocardial Infarction.
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Jain, Kinshuk, McCarley, Sarah C., Mukhtar, Ghazel, Ferlin, Anna, Fleming, Andrew, Morris-Rosendahl, Deborah J., and Shovlin, Claire L.
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MYOCARDIAL infarction , *HEREDITARY hemorrhagic telangiectasia , *TELANGIECTASIA , *GENETIC variation , *MISSENSE mutation , *GENETIC testing - Abstract
Hereditary haemorrhagic telangiectasia (HHT) is a vascular dysplasia inherited as an autosomal dominant trait, due to a single heterozygous loss-of-function variant, usually in ACVRL1 (encoding activin receptor-like kinase 1 [ALK1]), ENG (encoding endoglin [CD105]), or SMAD4. In a consecutive single-centre series of 37 positive clinical genetic tests performed in 2021–2023, a skewed distribution pattern was noted, with 30 of 32 variants reported only once, but ACVRL1 c.1231C>T (p.Arg411Trp) identified as the disease-causal gene in five different HHT families. In the same centre's non-overlapping 1992–2020 series where 110/134 (82.1%) HHT-causal variants were reported only once, ACVRL1 c.1231C>T (p.Arg411Trp) was identified in nine further families. In a 14-country, four-continent HHT Mutation Database where 181/250 (72.4%) HHT-causal variants were reported only once, ACVRL1 c.1231C>T (p.Arg411Trp) was reported by 12 different laboratories, the adjacent ACVRL1 c.1232G>A (p.Arg411Gln) by 14, and ACVRL1 c.1120C>T (p.Arg374Trp) by 18. Unlike the majority of HHT-causal ACVRL1 variants, these encode ALK1 protein that reaches the endothelial cell surface but fails to signal. Six variants of this type were present in the three series and were reported 6.8–25.5 (mean 8.9) times more frequently than the other ACVRL1 missense variants (all p-values < 0.0039). Noting lower rates of myocardial infarction reported in HHT, we explore potential mechanisms, including a selective paradigm relevant to ALK1′s role in the initiating event of atherosclerosis, where a plausible dominant negative effect of these specific variants can be proposed. In conclusion, there is an ~9-fold excess of kinase-inactive, cell surface-expressed ACVRL1/ALK1 pathogenic missense variants in HHT. The findings support further examination of differential clinical and cellular phenotypes by HHT causal gene molecular subtypes. [ABSTRACT FROM AUTHOR]
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- 2024
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17. Regulatory NADH dehydrogenase‐like complex optimizes C4 photosynthetic carbon flow and cellular redox in maize.
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Zhang, Qiqi, Tian, Shilong, Chen, Genyun, Tang, Qiming, Zhang, Yijing, Fleming, Andrew J., Zhu, Xin‐Guang, and Wang, Peng
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CALVIN cycle , *CARBON 4 photosynthesis , *ELECTRON transport , *COMPARATIVE method , *OXIDATION-reduction reaction - Abstract
Summary: C4 plants typically operate a CO2 concentration mechanism from mesophyll (M) cells into bundle sheath (BS) cells. NADH dehydrogenase‐like (NDH) complex is enriched in the BS cells of many NADP‐malic enzyme (ME) type C4 plants and is more abundant in C4 than in C3 plants, but to what extent it is involved in the CO2 concentration mechanism remains to be experimentally investigated.We created maize and rice mutants deficient in NDH function and then used a combination of transcriptomic, proteomic, and metabolomic approaches for comparative analysis.Considerable decreases in growth, photosynthetic activities, and levels of key photosynthetic proteins were observed in maize but not rice mutants. However, transcript abundance for many cyclic electron transport (CET) and Calvin–Benson cycle components, as well as BS‐specific C4 enzymes, was increased in maize mutants. Metabolite analysis of the maize ndh mutants revealed an increased NADPH : NADP ratio, as well as malate, ribulose 1,5‐bisphosphate (RuBP), fructose 1,6‐bisphosphate (FBP), and photorespiration intermediates.We suggest that by optimizing NADPH and malate levels and adjusting NADP‐ME activity, NDH functions to balance metabolic and redox states in the BS cells of maize (in addition to ATP supply), coordinating photosynthetic transcript abundance and protein content, thus directly regulating the carbon flow in the two‐celled C4 system of maize. [ABSTRACT FROM AUTHOR]
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- 2024
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18. Beyond gene-disease validity: capturing structured data on inheritance, allelic requirement, disease-relevant variant classes, and disease mechanism for inherited cardiac conditions.
- Author
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Josephs, Katherine S., Roberts, Angharad M., Theotokis, Pantazis, Walsh, Roddy, Ostrowski, Philip J., Edwards, Matthew, Fleming, Andrew, Thaxton, Courtney, Roberts, Jason D., Care, Melanie, Zareba, Wojciech, Adler, Arnon, Sturm, Amy C., Tadros, Rafik, Novelli, Valeria, Owens, Emma, Bronicki, Lucas, Jarinova, Olga, Callewaert, Bert, and Peters, Stacey
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HEREDITY , *GENETIC disorders , *ALLELES - Abstract
Background: As the availability of genomic testing grows, variant interpretation will increasingly be performed by genomic generalists, rather than domain-specific experts. Demand is rising for laboratories to accurately classify variants in inherited cardiac condition (ICC) genes, including secondary findings. Methods: We analyse evidence for inheritance patterns, allelic requirement, disease mechanism and disease-relevant variant classes for 65 ClinGen-curated ICC gene-disease pairs. We present this information for the first time in a structured dataset, CardiacG2P, and assess application in genomic variant filtering. Results: For 36/65 gene-disease pairs, loss of function is not an established disease mechanism, and protein truncating variants are not known to be pathogenic. Using the CardiacG2P dataset as an initial variant filter allows for efficient variant prioritisation whilst maintaining a high sensitivity for retaining pathogenic variants compared with two other variant filtering approaches. Conclusions: Access to evidence-based structured data representing disease mechanism and allelic requirement aids variant filtering and analysis and is a pre-requisite for scalable genomic testing. [ABSTRACT FROM AUTHOR]
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- 2023
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19. Charge drive with active DC stabilization for linearization of piezoelectric hysteresis.
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Fleming, Andrew
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ELECTRONIC linearization , *HYSTERESIS , *PIEZOELECTRIC actuators , *PERFORMANCE evaluation , *ELECTRIC capacity , *TRANSIENT analysis - Abstract
Charge drive circuits can significantly reduce piezoelectric nonlinearity; however, they are rarely used in practice because of their limited low-frequency performance, their dependence of voltage gain on the load capacitance, and their requirement for time-consuming tuning procedures. In this report, a new charge drive circuit is proposed that uses a controlled current source with voltage feedback to stabilize the low-frequency behavior. This approach eliminates many of the present difficulties and allows extremely low transition frequencies without a long transient response. Experimental results demonstrate that the proposed charge amplifier can effectively reduce piezoelectric hysteresis and creep to less than 1.3% at scan-rates of 10, 1, and 0.1 Hz. [ABSTRACT FROM AUTHOR]
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- 2013
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20. Elevated CO2 Priming as a Sustainable Approach to Increasing Rice Tiller Number and Yield Potential.
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Sloan, Jennifer M., Mujab, Azzami Adam Muhamad, Mashitah, Jusoh, Zulkarami, Berahim, Wilson, Matthew J., Toh, Liang Su, Nur Zahirah, A. Jalil, Afiq, Kamaruzali, Asyraf, Ahmad Tajuddin, Zhu, Xin-Guang, Yaapar, Nazmin, and Fleming, Andrew J.
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CULTIVATORS , *PLEUROTUS ostreatus , *RICE farmers , *ORDER picking systems , *MYCELIUM , *RICE farming - Abstract
Tillering and yield are linked in rice, with significant efforts being invested to understand the genetic basis of this phenomenon. However, in addition to genetic factors, tillering is also influenced by the environment. Exploiting experiments in which seedlings were first grown in elevated CO2 (eCO2) before transfer and further growth under ambient CO2 (aCO2) levels, we found that even moderate exposure times to eCO2 were sufficient to induce tillering in seedlings, which was maintained in plants grown to maturity plants in controlled environment chambers. We then explored whether brief exposure to eCO2 (eCO2 priming) could be implemented to regulate tiller number and yield in the field. We designed a cost-effective growth system, using yeast to increase the CO2 level for the first 24 days of growth, and grew these seedlings to maturity in semi-field conditions in Malaysia. The increased growth caused by eCO2 priming translated into larger mature plants with increased tillering, panicle number, and improved grain filling and 1000 grain weight. In order to make the process more appealing to conventional rice farmers, we then developed a system in which fungal mycelium was used to generate the eCO2 via respiration of sugars derived by growing the fungus on lignocellulosic waste. Not only does this provide a sustainable source of CO2, it also has the added financial benefit to farmers of generating economically valuable oyster mushrooms as an end-product of mycelium growth. Our experiments show that the system is capable of generating sufficient CO2 to induce increased tillering in rice seedlings, leading eventually to 18% more tillers and panicles in mature paddy-grown crop. We discuss the potential of eCO2 priming as a rapidly implementable, broadly applicable and sustainable system to increase tillering, and thus yield potential in rice. [ABSTRACT FROM AUTHOR]
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- 2023
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21. Origins and Evolution of Stomatal Development.
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Chater, Caspar C. C., Caine, Robert S., Fleming, Andrew J., and Gray, Julie E.
- Abstract
The fossil record suggests stomata-like pores were present on the surfaces of land plants over 400 million years ago. Whether stomata arose once or whether they arose independently across newly evolving land plant lineages has long been a matter of debate. In Arabidopsis, a genetic toolbox has been identified that tightly controls stomatal development and patterning. This includes the basic helix-loop-helix (bHLH) transcription factors SPEECHLESS (SPCH), MUTE, FAMA, and ICE/SCREAMs (SCRMs), which promote stomatal formation. These factors are regulated via a signaling cascade, which includes mobile EPIDERMAL PATTERNING FACTOR (EPF) peptides to enforce stomatal spacing. Mosses and hornworts, the most ancient extant lineages to possess stomata, possess orthologs of these Arabidopsis (Arabidopsis thaliana) stomatal toolbox genes, and manipulation in the model bryophyte Physcomitrella patens has shown that the bHLH and EPF components are also required for moss stomatal development and patterning. This supports an ancient and tightly conserved genetic origin of stomata. Here, we review recent discoveries and, by interrogating newly available plant genomes, we advance the story of stomatal development and patterning across land plant evolution. Furthermore, we identify potential orthologs of the key toolbox genes in a hornwort, further supporting a single ancient genetic origin of stomata in the ancestor to all stomatous land plants. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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22. Defining the scope for altering rice leaf anatomy to improve photosynthesis: a modelling approach.
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Xiao, Yi, Sloan, Jen, Hepworth, Chris, Fradera‐Soler, Marc, Mathers, Andrew, Thorley, Rachel, Baillie, Alice, Jones, Hannah, Chang, Tiangen, Chen, Xingyuan, Yaapar, Nazmin, Osborne, Colin P., Sturrock, Craig, Mooney, Sacha J., Fleming, Andrew J., and Zhu, Xin‐Guang
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LEAF anatomy , *PHOTOSYNTHESIS - Abstract
Summary: Leaf structure plays an important role in photosynthesis. However, the causal relationship and the quantitative importance of any single structural parameter to the overall photosynthetic performance of a leaf remains open to debate. In this paper, we report on a mechanistic model, eLeaf, which successfully captures rice leaf photosynthetic performance under varying environmental conditions of light and CO2.We developed a 3D reaction‐diffusion model for leaf photosynthesis parameterised using a range of imaging data and biochemical measurements from plants grown under ambient and elevated CO2 and then interrogated the model to quantify the importance of these elements.The model successfully captured leaf‐level photosynthetic performance in rice. Photosynthetic metabolism underpinned the majority of the increased carbon assimilation rate observed under elevated CO2 levels, with a range of structural elements making positive and negative contributions. Mesophyll porosity could be varied without any major outcome on photosynthetic performance, providing a theoretical underpinning for experimental data.eLeaf allows quantitative analysis of the influence of morphological and biochemical properties on leaf photosynthesis. The analysis highlights a degree of leaf structural plasticity with respect to photosynthesis of significance in the context of attempts to improve crop photosynthesis. [ABSTRACT FROM AUTHOR]
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- 2023
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23. Morphogenesis: Forcing the Tissue
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Fleming, Andrew
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MORPHOGENESIS , *TRANSCRIPTION factors , *PLANT cells & tissues , *EXTRACELLULAR matrix , *TISSUE mechanics - Abstract
Summary: How are the transcriptional events that control form actually transduced into the shape of an organism? Analysis of plant tissue mechanical properties shows that control of the extracellular matrix is key. [ABSTRACT FROM AUTHOR]
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- 2011
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24. Energy optimized aircraft and equipment systems.
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Fleming, Andrew J.
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AIRCRAFT carriers , *CONTRACTORS , *EQUIPMENT & supplies ,EDWARDS Air Force Base (Calif.) - Abstract
The article discusses developments and trends in energy optimized aircraft and equipment systems for 2012. The integrated vehicle energy technology (INVENT) program of the U.S. Air Force Research Laboratory is conducted under the triservice energy optimized aircraft (EOA) initiative. Some benefits of the EAO initiative are performance, energy and acquisition. INVENT and prime contractor Boeing have completed an integrated subsystem design of an Air Dominance Concept vehicle.
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- 2012
25. Buried treasure.
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Fleming, Andrew
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ARCHAEOLOGY , *NONFICTION - Abstract
The article reviews the book "The Passage of Arms: An Archaeological Analysis of Prehistoric Hoards and Votive Deposits," by Richard Bradley.
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- 1991
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26. TERT Expression in Wilms Tumor Is Regulated by Promoter Mutation or Hypermethylation, WT1, and N-MYC.
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Jablonowski, Carolyn M., Gil, Hyea Jin, Pinto, Emilia M., Pichavaram, Prahalathan, Fleming, Andrew M., Clay, Michael R., Hu, Dongli, Morton, Christopher L., Pruett-Miller, Shondra M., Hansen, Baranda S., Chen, Xiang, Jones, Karissa M. Dieseldorff, Liu, Yanling, Ma, Xiaotu, Yang, Jun, Davidoff, Andrew M., Zambetti, Gerard P., and Murphy, Andrew J.
- Subjects
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REVERSE transcriptase polymerase chain reaction , *TELOMERES , *GENETIC mutation , *XENOGRAFTS , *SEQUENCE analysis , *ONCOGENES , *TELOMERASE , *HEALTH outcome assessment , *GENE expression , *NEPHROBLASTOMA , *METHYLATION , *CANCER genes , *CELL proliferation , *DESCRIPTIVE statistics , *POLYMERASE chain reaction , *STATISTICAL correlation , *CELL lines - Abstract
Simple Summary: The telomerase enzyme adds repetitive genetic sequences to the ends of chromosomes called telomeres to prevent cellular senescence. Gain of telomerase function is one of the hallmarks of human cancer. The telomerase protein is coded by the gene TERT and increased TERT RNA levels have been associated with disease relapse in Wilms tumor, the most common kidney cancer of childhood. This study aimed to determine the mechanisms of increased TERT expression in Wilms tumor. This study found mutations in the TERT promoter, increased methylation of the TERT promoter, and genomic copy number amplifications of TERT as potential mechanisms of TERT activation. Conversely, this study found that inactivating WT1 mutation was associated with low TERT RNA levels and telomerase activity. N-MYC overexpression in Wilms tumor cells resulted in increased TERT promoter activity and TERT transcription. TERT transcription is associated with molecular and histologic subgroups in Wilms tumor and telomere-targeted therapies warrant future investigation. Increased TERT mRNA is associated with disease relapse in favorable histology Wilms tumor (WT). This study sought to understand the mechanism of increased TERT expression by determining the association between TERT and WT1 and N-MYC, two proteins important in Wilms tumor pathogenesis that have been shown to regulate TERT expression. Three out of 45 (6.7%) WTs and the corresponding patient-derived xenografts harbored canonical gain-of-function mutations in the TERT promoter. This study identified near ubiquitous hypermethylation of the TERT promoter region in WT compared to normal kidney. WTs with biallelic inactivating mutations in WT1 (7/45, 15.6%) were found to have lower TERT expression by RNA-seq and qRT-PCR and lower telomerase activity determined by the telomerase repeat amplification protocol. Anaplastic histology and increased percentage of blastema were positively correlated with higher TERT expression and telomerase activity. In vitro shRNA knockdown of WT1 resulted in decreased expression of TERT, reduced colony formation, and decreased proliferation of WiT49, an anaplastic WT cell line with wild-type WT1. CRISPR-Cas9-mediated knockout of WT1 resulted in decreased expression of telomere-related gene pathways. However, an inducible Wt1-knockout mouse model showed no relationship between Wt1 knockout and Tert expression in normal murine nephrogenesis, suggesting that WT1 and TERT are coupled in transformed cells but not in normal kidney tissues. N-MYC overexpression resulted in increased TERT promoter activity and TERT transcription. Thus, multiple mechanisms of TERT activation are involved in WT and are associated with anaplastic histology and increased blastema. This study is novel because it identifies potential mechanisms of TERT activation in Wilms tumor that could be of therapeutic interests. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
27. Gall formation in clubroot-infected Arabidopsis results from an increase in existing meristematic activities of the host but is not essential for the completion of the pathogen life cycle.
- Author
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Malinowski, Robert, Smith, Jody A., Fleming, Andrew J., Scholes, Julie D., and Rolfe, Stephen A.
- Subjects
- *
ARABIDOPSIS thaliana , *PATHOGENIC microorganisms , *LIFE cycles (Biology) , *PLANT parenchyma , *PHLOEM , *CAMBIUM , *STEM cells - Abstract
Plasmodiophora brassicae (clubroot) infection leads to reprogramming of host development resulting in the formation of characteristic galls. In this work we explored the cellular events that underly gall formation in Arabidopsis thaliana with the help of molecular markers of cell division ( CYCB1:GUS) and meristematic activity ( ANT:GUS). Our results show that gall development involved the amplification of existing meristematic activities within the vascular cambium (VC) and phloem parenchyma (PP) cells in the region of the hypocotyl. Additionally we found that the increase in VC activity and prolonged maintenance of cambial-derived cells in a meristematic state was crucial for gall formation; disruption of the VC activity significantly decreased the gall size. Gall formation also perturbed vascular development with a significant reduction in xylem and increase in PP in infected plants. This situation was reflected in a decrease in transcripts of key factors promoting xylogenesis (VND6, VND7 and MYB46) and an increase in those promoting phloem formation and function (APL, SUC2). Finally we show, using the cell cycle inhibitor ICK1/KRP1 and a cle41 mutant with altered regulation of cambial stem cell maintenance and differentiation, that a decrease in gall formation did not prevent pathogen development. This finding demonstrates that although gall formation is a typical symptom of the disease and influences numbers of spores produced, it is not required for completion of the pathogen life cycle. Together, these results provide an insight into the relationship of the cellular events that accompany Plasmodiophora infection and their role in disease progression. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
28. A Phox2- and Hand2-Dependent Hand1 cis-Regulatory Element Reveals a Unique Gene Dosage Requirement for Hand2 during Sympathetic Neurogenesis.
- Author
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Vincentz, Joshua W., VanDusen, Nathan J., Fleming, Andrew B., Rubart, Michael, Firulli, Beth A., Howard, Marthe J., and Firulli, Anthony B.
- Subjects
- *
DEVELOPMENTAL neurobiology , *NEUROGENETICS , *CELL differentiation , *TRANSCRIPTION factors , *CHROMATIN , *GENE expression , *MOLECULAR genetics - Abstract
Neural crest cell specification and differentiation to a sympathetic neuronal fate serves as an important model for neurogenesis and depends upon the function of both bHLH transcription factors, notably Hand2, and homeodomain transcription factors, including Phox2b. Here, we define a 1007 bp cis-regulatory element 5' of the Hand1 gene sufficient to drive reporter expression within the sympathetic chain of transgenic mice. Comparative genomic analyses uncovered evolutionarily conserved consensus-binding sites within this element, which chromatin immunoprecipitation and electrophoretic mobility shift assays confirm are bound by Hand2 and Phox2b. Mutational analyses revealed that the conserved Phox2 and E-box binding sites are necessary for proper cis-regulatory element activity, and expression analyses on both Hand2 conditionally null and hypomorphic backgrounds demonstrate that Hand2 is required for reporter activation in a gene dosage-dependent manner.Wedemonstrate that Hand2 and Hand1 differentially bind the E-boxes in this cis-regulatory element, establishing molecular differences between these two factors. Finally, we demonstrate that Hand1 is dispensable for normal tyrosine hydroxylase (TH) and dopamine &bgr;-hydroxylase (DBH) expression in sympathetic neurons, even when Hand2 gene dosage is concurrently reduced by half. Together, these data define a tissue-specific Hand1 cis-regulatory element controlled by two factors essential for the development of the sympathetic nervous system and provide in vivo regulatory evidence to support previous findings that Hand2, rather than Hand1, is predominantly responsible for regulating TH, DBH, and Hand1 expression in developing sympathetic neurons. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
29. Targeted manipulation of leaf form via local growth repression.
- Author
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Malinowski, Robert, Kasprzewska, Ania, and Fleming, Andrew J.
- Subjects
- *
PLANT cells & tissues , *CELL proliferation , *GROWTH factors , *GENE targeting , *PROTEIN kinases , *NUCLEOTIDE sequence , *MORPHOGENESIS , *GENE expression in plants - Abstract
A classical view is that leaf shape is the result of local promotion of growth linked to cell proliferation. However, an alternative hypothesis is that leaf form is the result of local repression of growth in an otherwise growing system. Here we show that leaf form can indeed be manipulated in a directed fashion by local repression of growth. We show that targeting expression of an inhibitor of a cyclin-dependent kinase ( KRP1) to the sinus area of developing leaves of Arabidopsis leads to local growth repression and the formation of organs with extreme lobing, including generation of leaflet-like organs. Directing KRP1 expression to other regions of the leaf using an miRNA target sequence tagging approach also leads to predictable novel leaf forms, and repression of growth in the leaf margin blocks the outgrowth of lobes, leading to a smoother perimeter. In addition, we show that decreased growth around the perimeter and across the leaf abaxial surface leads to a change in 3D form, as predicted by mechanical models of leaf growth. Our analysis provides experimental evidence that local repression of growth influences leaf shape, suggesting that it could be part of the mechanism of morphogenesis in plants in the context of an otherwise growing system. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
30. The ABC transporter SpTUR2 confers resistance to the antifungal diterpene sclareol.
- Author
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van den Brûle, Sybille, Müller, Axel, Fleming, Andrew J, and Smart, Cheryl C
- Subjects
- *
ADENOSINE triphosphate , *DITERPENES , *ARABIDOPSIS thaliana , *ABSCISIC acid - Abstract
Summary PDR5-like proteins represent one group of the ABC superfamily of transporters. Members of this group are present in plants and, due to the function of PDR5-related proteins in fungi in the excretion of xenobiotics (including antifungal agents), it has been proposed that they might play a similar role in plants in the response to and detoxification of herbicides and fungicides. However, until now no functional data has been presented showing an altered plant response to any herbicide or fungicide as a result of manipulating the expression of a PDR5-like gene in plants. In this paper, we show that the plant SpTUR2 PDR5-like ABC transporter is localised to the plasma membrane and that expression of this protein in Arabidopsis leads to the acquisition of resistance to the diterpenoid antifungal agent sclareol. These data both define a possible endogenous substrate for this transporter and highlight the potential of manipulating plant chemical resistance via modulating the expression of specific PDR5-like transporters. [ABSTRACT FROM AUTHOR]
- Published
- 2002
- Full Text
- View/download PDF
31. Novel marker genes for early leaf development indicate spatial regulation of carbohydrate metabolism within the apical meristem.
- Author
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Pien, Stéphane, Wyrzykowska, Joanna, and Fleming, Andrew J.
- Subjects
- *
MERISTEMS , *LEAF development , *PLANT development , *CARBOHYDRATE metabolism - Abstract
Summary To identify genes expressed at the earliest stages of leaf development, we have performed a differential display analysis using portions of meristems destined to form leaves. Our analysis led to the identification of five genes showing an asymmetric pattern of gene expression within the meristem associated with leaf formation. Surprisingly, three of these genes encoded enzymes involved in carbohydrate metabolism (ADPglucose pyrophosphorylase, sucrose synthase and an SNF1-like kinase). Furthermore, specific transcript patterns were responsive to specific sugar and hormonal treatments. The other two genes identified encoded a Phantastica-like myb transcription factor (associated with the acquisition of leaf dorsiventrality) and CYP85 (a cytochrome P450, which plays a pivotal role in brassinolide metabolism). These data, firstly, identify a novel set of marker genes for the analysis of the earliest stages of leaf formation. Secondly, the function of the proteins encoded by these genes and their expression patterns within the meristem indicate that carbohydrate metabolism is spatially regulated within a tissue involved in key developmental processes. Finally, our data provide the first indication of an asymmetry in gene expression related to hormone biosynthesis in the meristem. [ABSTRACT FROM AUTHOR]
- Published
- 2001
- Full Text
- View/download PDF
32. Changes in prey fields increase the potential for spatial overlap between gentoo penguins and a krill fishery within a marine protected area.
- Author
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Ratcliffe, Norman, Deagle, Bruce, Love, Kieran, Polanowski, Andrea, Fielding, Sophie, Wood, Andrew G., Hill, Simeon, Grant, Susie, Belchier, Mark, Fleming, Andrew, and Hall, Jonathan
- Subjects
- *
MARINE parks & reserves , *KRILL , *FISHERIES , *PENGUINS , *BYCATCHES , *FISHERY management - Abstract
AIM: Management of competition with predators is an important consideration for fisheries, particularly within marine protected areas (MPAs) where conservation is a primary objective. We aimed to test whether static no‐take zones within a large, sustainable‐use MPA prevented overlap between gentoo penguins and a krill fishery during two winters with contrasting prey fields. LOCATION: South Georgia, Southwest Atlantic Ocean. METHOD: We used satellite tracking (N = 16, June–September 2018) to describe gentoo penguin movements and distribution and quantified their overlap with the MPA's no‐take zone (NTZ) and the krill fishing grounds. DNA metabarcoding of scats (N = 220, April–September 2018) was used to quantify diet. RESULTS: When krill were at moderate densities and evenly distributed in 2001, gentoo penguins would have spent all of their time within the 12 NM NTZ, but when availability was low in 2018, they spent 46.3% of their time outside the NTZ and 9.6% within the krill fishing grounds. The extension of the NTZ to 30 km in response to this finding would have produced a 14.9% increase in protection for penguins and displaced 4% of fishery hauls. Gentoo penguin diet comprised 25.8% krill, which is lower than in the late 1980s but more than in 2009. MAIN CONCLUSIONS: Gentoo penguins extend their foraging range when krill is scarce, which increases the potential for spatial overlap with the krill fishery during periods of nutritional stress. Current regulations allow for expansion of both extent and catches by the krill fishery and, should this occur, gentoo penguins may face heightened risks from competition. A dynamic ocean management framework, that extends closed areas in response to near real‐time data on penguin movements and krill density estimates, may reduce the potential for competition in this sustainable‐use MPA while allowing a profitable krill fishery. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
33. St Edmund, King and Martyr: Changing Images of a Medieval Saint.
- Author
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Fleming, Andrew
- Subjects
- *
SAINTS , *NONFICTION - Abstract
The article reviews the book "St Edmund, King and Martyr: Changing Images of a Medieval Saint," edited by Anthony Bale.
- Published
- 2010
- Full Text
- View/download PDF
34. BOOK REVIEWS.
- Author
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Fleming, Andrew
- Subjects
- LANDLESS & Hungry (Book)
- Abstract
Reviews the book `Landless and Hungry: Access to Land in Early and Tradional Societies,' edited by B. Haring and R. De Maaijer.
- Published
- 2000
- Full Text
- View/download PDF
35. Reduced stomatal density in bread wheat leads to increased water-use efficiency.
- Author
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Dunn, Jessica, Hunt, Lee, Afsharinafar, Mana, Meselmani, Moaed Al, Mitchell, Alice, Howells, Rhian, Wallington, Emma, Fleming, Andrew J, and Gray, Julie E
- Subjects
- *
WHEAT breeding , *PLANT breeding , *DENSITY , *WHEAT , *GRAIN yields , *PLANT spacing , *WINTER wheat - Abstract
Wheat is a staple crop, frequently cultivated in water-restricted environments. Improving crop water-use efficiency would be desirable if grain yield can be maintained. We investigated whether a decrease in wheat stomatal density via the manipulation of epidermal patterning factor (EPF) gene expression could improve water-use efficiency. Our results show that severe reductions in stomatal density in EPF-overexpressing wheat plants have a detrimental outcome on yields. However, wheat plants with a more moderate reduction in stomatal density (i.e. <50% reduction in stomatal density on leaves prior to tillering) had yields indistinguishable from controls, coupled with an increase in intrinsic water-use efficiency. Yields of these moderately reduced stomatal density plants were also comparable with those of control plants under conditions of drought and elevated CO2. Our data demonstrate that EPF-mediated control of wheat stomatal development follows that observed in other grasses, and we identify the potential of stomatal density as a tool for breeding wheat plants that are better able to withstand water-restricted environments without yield loss. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
36. Modeling of soft fluidic actuators using fluid–structure interaction simulations with underwater applications.
- Author
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Xavier, Matheus S., Harrison, Simon M., Howard, David, Yong, Yuen K., and Fleming, Andrew J.
- Subjects
- *
FLUID-structure interaction , *ACTUATORS , *DEAD loads (Mechanics) , *FLUID flow , *SOFT robotics , *FISHWAYS , *PNEUMATICS , *REMOTE submersibles - Abstract
Soft robots have been developed for a variety of applications including gripping, locomotion, wearables and medical devices. For the majority of soft robots, actuation is performed using pneumatics or hydraulics. Many previous works have addressed the modeling of these fluid-driven soft robots using static finite element simulations where the pressure inside the actuator is assumed to be constant and uniform. The assumption of constant internal pressure is a useful simplification but introduces significant errors during events such as pressurization, depressurization, and transient loads from a liquid environment. Applications that use soft actuators for locomotion or propulsion operate using a sequence of transient events, so accurate simulation of these events is critical to optimizing performance. To improve the simulation of soft fluidic actuators and enable the modeling of both internal and external fluid flow in underwater applications, this work describes a fully-coupled, three-dimensional fluid–structure interaction simulation approach, where the pressure and flow dynamics are explicitly solved. This approach provides a realistic simulation of soft actuators in fluid environments, and permits the optimization of transient responses, which may be due to a combination of environmental fluid loads and non-uniform pressurization. The proposed methods are demonstrated in a number of case studies and experiments for a range of actuation and both internal and external inlet flow configurations, including bending actuators, a soft robotic fish fin for propulsion, and experimental results of a bending actuator in a high-speed fluid, which correlate closely with simulations. The proposed approach is expected to assist in the design, modeling, and optimization of bioinspired soft robots in underwater applications. [Display omitted] • 3D fluid–structure interaction simulations (FSI) for soft fluidic actuators are developed. • This approach is used to model both internal and external flow and pressure dynamics. • Realistic simulation of soft actuators in fluid environments such as underwater. • A series of comparison experiments and FSI analysis are presented and discussed. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
37. BMP-Responsive Protease HtrAl Is Differentially Expressed in Astrocytes and Regulates Astrocytic Development and Injury Response.
- Author
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Chen, Jessie, Van Gulden, Stephanie, McGuire, Tammy L., Fleming, Andrew C., Chio Oka, Kessler, John A., and Chian-Yu Peng
- Subjects
- *
BONE morphogenetic proteins , *ASTROCYTES , *ION exchange (Chemistry) , *NEUROTRANSMITTER uptake inhibitors , *CHONDROITIN sulfates , *PROTEOGLYCANS , *ENDOTHELIAL cells - Abstract
Astrocytes perform a wide array of physiological functions, including structural support, ion exchange, and neurotransmitter uptake. Despite this diversity, molecular markers that label subpopulations of astrocytes are limited, and mechanisms that generate distinct astrocyte subtypes remain unclear. Here we identified serine protease high temperature requirement A 1 (HtrAl), a bone morphogenetic protein 4 signaling regulated protein, as a novel marker of forebrain astrocytes, but not of neural stem cells, in adult mice of both sexes. Genetic deletion of HtrAl during gliogenesis accelerates astrocyte differentiation. In addition, ablation of HtrAl in cultured astrocytes leads to altered chondroitin sulfate proteoglycan expression and inhibition of neurite extension, along with elevated levels of transforming growth fact or-(3 family proteins. Brain injury induces HtrA 1 expression in reactive astrocytes, and loss of HtrA 1 leads to an impairment in wound closure accompanied by increased proliferation of endothelial and immune cells. Our findings demonstrate that HtrAl is differentially expressed in adult mouse forebrain astrocytes, and that HtrAl plays important roles in astrocytic development and injury response. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
38. Cell density and airspace patterning in the leaf can be manipulated to increase leaf photosynthetic capacity.
- Author
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Lehmeier, Christoph, Pajor, Radoslaw, Lundgren, Marjorie R., Mathers, Andrew, Sloan, Jen, Bauch, Marion, Mitchell, Alice, Bellasio, Chandra, Green, Adam, Bouyer, Daniel, Schnittger, Arp, Sturrock, Craig, Osborne, Colin P., Rolfe, Stephen, Mooney, Sacha, and Fleming, Andrew J.
- Subjects
- *
CELL division , *CELL growth , *PHOTOSYNTHESIS , *ARABIDOPSIS thaliana , *MESOPHYLL tissue , *PHYSIOLOGY , *PLANTS - Abstract
The pattern of cell division, growth and separation during leaf development determines the pattern and volume of airspace in a leaf. The resulting balance of cellular material and airspace is expected to significantly influence the primary function of the leaf, photosynthesis, and yet the manner and degree to which cell division patterns affect airspace networks and photosynthesis remains largely unexplored. In this paper we investigate the relationship of cell size and patterning, airspace and photosynthesis by promoting and repressing the expression of cell cycle genes in the leaf mesophyll. Using micro CT imaging to quantify leaf cellular architecture and fluorescence/gas exchange analysis to measure leaf function, we show that increased cell density in the mesophyll of Arabidopsis can be used to increase leaf photosynthetic capacity. Our analysis suggests that this occurs both by increasing tissue density (decreasing the relative volume of airspace) and by altering the pattern of airspace distribution within the leaf. Our results indicate that cell division patterns influence the photosynthetic performance of a leaf, and that it is possible to engineer improved photosynthesis via this approach. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
39. Formation of the Stomatal Outer Cuticular Ledge Requires a Guard Cell Wall Proline-Rich Protein.
- Author
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Hunt, Lee, Amsbury, Samuel, Baillie, Alice, Movahedi, Mahsa, Mitchell, Alice, Afsharinafar, Mana, Swarup, Kamal, Denyer, Thomas, Hobbs, Jamie K., Swarup, Ranjan, Fleming, Andrew J., and Gray, Julie E.
- Abstract
Stomata are formed by a pair of guard cells which have thickened, elastic cell walls to withstand the large increases in turgor pressure that have to be generated to open the pore that they surround. We have characterized FOCL1, a guard cell-expressed, secreted protein with homology to Hyp-rich cell wall proteins. FOCL1-GFP localizes to the guard cell outer cuticular ledge and plants lacking FOCL1 produce stomata without a cuticular ledge. Instead the majority of stomatal pores are entirely covered over by a continuous fusion of the cuticle, and consequently plants have decreased levels of transpiration and display drought tolerance. The focl1 guard cells are larger and less able to reduce the aperture of their stomatal pore in response to closure signals suggesting that the flexibility of guard cell walls is impaired. FOCL1 is also expressed in lateral root initials where it aids lateral root emergence. We propose that FOCL1 acts in these highly specialized cells of the stomata and root to impart cell wall strength at high turgor and/or to facilitate interactions between the cell wall and the cuticle. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
40. Stomatal Function Requires Pectin De-methyl-esterification of the Guard Cell Wall.
- Author
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Amsbury, Sam, Hunt, Lee, Elhaddad, Nagat, Baillie, Alice, Lundgren, Marjorie, Verhertbruggen, Yves, Scheller, Henrik V., Knox, J. Paul, Fleming, Andrew J., and Gray, Julie E.
- Subjects
- *
PECTINS , *STOMATA , *GUARD cells (Plant anatomy) , *ESTERIFICATION , *METHYL groups , *CELLULAR mechanics - Abstract
Summary Stomatal opening and closure depends on changes in turgor pressure acting within guard cells to alter cell shape [ 1 ]. The extent of these shape changes is limited by the mechanical properties of the cells, which will be largely dependent on the structure of the cell walls. Although it has long been observed that guard cells are anisotropic due to differential thickening and the orientation of cellulose microfibrils [ 2 ], our understanding of the composition of the cell wall that allows them to undergo repeated swelling and deflation remains surprisingly poor. Here, we show that the walls of guard cells are rich in un-esterified pectins. We identify a pectin methylesterase gene, PME6 , which is highly expressed in guard cells and required for stomatal function. pme6-1 mutant guard cells have walls enriched in methyl-esterified pectin and show a decreased dynamic range in response to triggers of stomatal opening/closure, including elevated osmoticum, suggesting that abrogation of stomatal function reflects a mechanical change in the guard cell wall. Altered stomatal function leads to increased conductance and evaporative cooling, as well as decreased plant growth. The growth defect of the pme6-1 mutant is rescued by maintaining the plants in elevated CO 2 , substantiating gas exchange analyses, indicating that the mutant stomata can bestow an improved assimilation rate. Restoration of PME6 rescues guard cell wall pectin methyl-esterification status, stomatal function, and plant growth. Our results establish a link between gene expression in guard cells and their cell wall properties, with a corresponding effect on stomatal function and plant physiology. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
41. An ancestral stomatal patterning module revealed in the non-vascular land plant Physcomitrella patens.
- Author
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Caine, Robert S., Chater, Caspar C., Kamisugi, Yasuko, Cuming, Andrew C., Beerling, David J., Gray, Julie E., and Fleming, Andrew J.
- Subjects
- *
STOMATA , *PLANT development , *PLANT evolution , *ARABIDOPSIS , *PHYSCOMITRELLA patens , *NONVASCULAR plants - Abstract
The patterning of stomata plays a vital role in plant development and has emerged as a paradigm for the role of peptide signals in the spatial control of cellular differentiation. Research in Arabidopsis has identified a series of epidermal patterning factors (EPFs), which interact with an array of membrane-localised receptors and associated proteins (encoded by ERECTA and TMM genes) to control stomatal density and distribution. However, although it is well-established that stomata arose very early in the evolution of land plants, until now it has been unclear whether the established angiosperm stomatal patterning system represented by the EPF/ TMM/ERECTA module reflects a conserved, universal mechanism in the plant kingdom. Here, we use molecular genetics to show that the moss Physcomitrella patens has conserved homologues of angiosperm EPF, TMM and at least one ERECTA gene that function together to permit the correct patterning of stomata and that, moreover, elements of the module retain function when transferred to Arabidopsis. Our data characterise the stomatal patterning system in an evolutionarily distinct branch of plants and support the hypothesis that the EPF/TMM/ERECTA module represents an ancient patterning system. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
42. Combined Chlorophyll Fluorescence and Transcriptomic Analysis Identifies the P3/P4 Transition as a Key Stage in Rice Leaf Photosynthetic Development.
- Author
-
van Campen, Julia C., Yaapar, Muhammad N., Narawatthana, Supatthra, Lehmeier, Christoph, Wanchana, Samart, Thakur, Vivek, Chater, Caspar, Kelly, Steve, Rolfe, Stephen A., Quick, W. Paul, and Fleming, Andrew J.
- Subjects
- *
CHLOROPHYLL spectra , *TRANSCRIPTION factors , *HARVESTING , *RICE , *PHOTOSYNTHESIS , *COMPOSITION of leaves , *MOLECULAR biology - Abstract
Leaves are derived from heterotrophic meristem tissue that, at some point, must make the transition to autotrophy via the initiation of photosynthesis. However, the timing and spatial coordination of the molecular and cellular processes underpinning this switch are poorly characterized. Here, we report on the identification of a specific stage in rice (Oryza sativa) leaf development (P3/P4 transition) when photosynthetic competence is first established. Using a combined physiological and molecular approach, we show that elements of stomatal and vascular differentiation are coordinated with the onset of measurable light absorption for photosynthesis. Moreover, by exploring the response of the system to environmental perturbation, we show that the earliest stages of rice leaf development have significant plasticity with respect to elements of cellular differentiation of relevance for mature leaf photosynthetic performance. Finally, by performing an RNA sequencing analysis targeted at the early stages of rice leaf development, we uncover a palette of genes whose expression likely underpins the acquisition of photosynthetic capability. Our results identify the P3/P4 transition as a highly dynamic stage in rice leaf development when several processes for the initiation of photosynthetic competence are coordinated. As well as identifying gene targets for future manipulation of rice leaf structure/function, our data highlight a developmental window during which such manipulations are likely to be most effective. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
43. Assessing the effectiveness of specially protected areas for conservation of Antarctica's botanical diversity.
- Author
-
Hughes, Kevin A., Ireland, Louise C., Convey, Peter, and Fleming, Andrew H.
- Subjects
- *
VEGETATION & climate , *PLANT communities , *TRAMPLING , *INTRODUCED species - Abstract
Vegetation is sparsely distributed over Antarctica's ice-free ground, and distinct plant communities are present in each of the continent's 15 recently identified Antarctic Conservation Biogeographic Regions (ACBRs). With rapidly increasing human activity in Antarctica, terrestrial plant communities are at risk of damage or destruction by trampling, overland transport, and infrastructure construction and from the impacts of anthropogenically introduced species, as well as uncontrollable pressures such as fur seal (Arctocephalus gazella) activity and climate change. Under the Protocol on Environmental Protection to the Antarctic Treaty, the conservation of plant communities can be enacted and facilitated through the designation of Antarctic Specially Protected Areas (ASPAs). We examined the distribution within the 15 ACBRs of the 33 ASPAs whose explicit purpose includes protecting macroscopic terrestrial flora. We completed the first survey using normalized difference vegetation index (NDVI) satellite remote sensing to provide baseline data on the extent of vegetation cover in all ASPAs designated for plant protection in Antarctica. Large omissions in the protection of Antarctic botanical diversity were found. There was no protection of plant communities in 6 ACBRs, and in another 6, <0.4% of the ACBR area was included in an ASPA that protected vegetation. Protected vegetation cover within the 33 ASPAs totaled 16.1 km2 for the entire Antarctic continent; over half was within a single protected area. Over 96% of the protected vegetation was contained in 2 ACBRs, which together contributed only 7.8% of the continent's ice-free ground. We conclude that Antarctic botanical diversity is clearly inadequately protected and call for systematic designation of ASPAs protecting plant communities by the Antarctic Treaty Consultative Parties, the members of the governing body of the continent. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
44. Auxin influx importers modulate serration along the leaf margin.
- Author
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Kasprzewska, Ania, Carter, Ross, Swarup, Ranjan, Bennett, Malcolm, Monk, Nick, Hobbs, Jamie K., and Fleming, Andrew
- Subjects
- *
LEAF anatomy , *AUXIN , *PLANT embryology , *ARABIDOPSIS , *PLANT growth , *GENETIC transcription in plants - Abstract
Leaf shape in Arabidopsis is modulated by patterning events in the margin that utilize a PIN-based auxin exporter/CUC2 transcription factor system to define regions of promotion and retardation of growth, leading to morphogenesis. In addition to auxin exporters, leaves also express auxin importers, notably members of the AUX1/ LAX family. In contrast to their established roles in embryogenesis, lateral root and leaf initiation, the function of these transporters in leaf development is poorly understood. We report that three of these genes ( AUX1, LAX1 and LAX2) show specific and dynamic patterns of expression during early leaf development in Arabidopsis, and that loss of expression of all three genes is required for observation of a phenotype in which morphogenesis (serration) is decreased. We used these expression patterns and mutant phenotypes to develop a margin-patterning model that incorporates an AUX1/ LAX1/ LAX2 auxin import module that influences the extent of leaf serration. Testing of this model by margin-localized expression of axr3-1 ( AXR17) provides further insight into the role of auxin in leaf morphogenesis. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
45. Altering arabinans increases Arabidopsis guard cell flexibility and stomatal opening.
- Author
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Carroll, Sarah, Amsbury, Sam, Durney, Clinton H., Smith, Richard S., Morris, Richard J., Gray, Julie E., and Fleming, Andrew J.
- Subjects
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STOMATA , *CELLULAR mechanics , *ATOMIC force microscopy , *ARABIDOPSIS , *GAS exchange in plants , *PLANT-water relationships - Abstract
Stomata regulate plant water use and photosynthesis by controlling leaf gas exchange. They do this by reversibly opening the pore formed by two adjacent guard cells, with the limits of this movement ultimately set by the mechanical properties of the guard cell walls and surrounding epidermis. 1,2 A body of evidence demonstrates that the methylation status and cellular patterning of pectin wall polymers play a core role in setting the guard cell mechanical properties, with disruption of the system leading to poorer stomatal performance. 3–6 Here we present genetic and biochemical data showing that wall arabinans modulate guard cell flexibility and can be used to engineer stomata with improved performance. Specifically, we show that a short-chain linear arabinan epitope associated with the presence of rhamnogalacturonan I in the guard cell wall is required for full opening of the stomatal pore. Manipulations leading to the novel accumulation of longer-chain arabinan epitopes in guard cell walls led to an increase in the maximal pore aperture. Using computational modeling combined with atomic force microscopy, we show that this phenotype reflected a decrease in wall matrix stiffness and, consequently, increased flexing of the guard cells under turgor pressure, generating larger, rounder stomatal pores. Our results provide theoretical and experimental support for the conclusion that arabinan side chains of pectin modulate guard cell wall stiffness, setting the limits for cell flexing and, consequently, pore aperture, gas exchange, and photosynthetic assimilation. [Display omitted] • Cell walls in stomata have a distinct composition of arabinans • Increasing the level of a specific arabinan makes the walls more flexible • Stomata with more flexible walls can open wider • Under high CO 2 , more flexible, wider stomata increase carbon assimilation rate The degree of stomatal opening is set by the mechanical properties of the guard cell walls. Carroll et al. show that wall flexibility is set by the arabinan composition, and that by manipulating arabinan polymers it is possible to engineer stomata with increased opening under elevated CO 2 , leading to increased carbon assimilation. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
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46. Conservation of Male Sterility 2 function during spore and pollen wall development supports an evolutionarily early recruitment of a core component in the sporopollenin biosynthetic pathway.
- Author
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Wallace, Simon, Chater, Caspar C., Kamisugi, Yasuko, Cuming, Andrew C., Wellman, Charles H., Beerling, David J., and Fleming, Andrew J.
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MALE sterility in plants , *SPOROPOLLENIN , *ARABIDOPSIS thaliana , *PLANT spores , *EXINE , *BIOSYNTHESIS , *PHYSCOMITRELLA patens - Abstract
The early evolution of plants required the acquisition of a number of key adaptations to overcome physiological difficulties associated with survival on land. One of these was a tough sporopollenin wall that enclosed reproductive propagules and provided protection from desiccation and UV- B radiation. All land plants possess such walled spores (or their derived homologue, pollen)., We took a reverse genetics approach, consisting of knock-out and complementation experiments to test the functional conservation of the sporopollenin-associated gene MALE STERILTY 2 (which is essential for pollen wall development in Arabidopsis thaliana) in the bryophyte Physcomitrella patens., Knock-outs of a putative moss homologue of the A. thaliana MS2 gene, which is highly expressed in the moss sporophyte, led to spores with highly defective walls comparable to that observed in the A. thaliana ms2 mutant, and extremely compromised germination. Conversely, the moss MS2 gene could not rescue the A. thaliana ms2 phenotype., The results presented here suggest that a core component of the biochemical and developmental pathway required for angiosperm pollen wall development was recruited early in land plant evolution but the continued increase in pollen wall complexity observed in angiosperms has been accompanied by divergence in MS2 gene function. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
47. Open Access Data in Polar and Cryospheric Remote Sensing.
- Author
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Pope, Allen, Gareth Rees, W., Fox, Adrian J., and Fleming, Andrew
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CRYOSPHERE , *REMOTE-sensing images , *AEROSPACE telemetry , *GEOPHYSICAL prospecting , *DETECTORS - Abstract
Abstract: This paper aims to introduce the main types and sources of remotely sensed data that are freely available and have cryospheric applications. We describe aerial and satellite photography, satellite-borne visible, near-infrared and thermal infrared sensors, synthetic aperture radar, passive microwave imagers and active microwave scatterometers. We consider the availability and practical utility of archival data, dating back in some cases to the 1920s for aerial photography and the 1960s for satellite imagery, the data that are being collected today and the prospects for future data collection; in all cases, with a focus on data that are openly accessible. Derived data products are increasingly available, and we give examples of such products of particular value in polar and cryospheric research. We also discuss the availability and applicability of free and, where possible, open-source software tools for reading and processing remotely sensed data. The paper concludes with a discussion of open data access within polar and cryospheric sciences, considering trends in data discoverability, access, sharing and use. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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48. Increased leaf mesophyll porosity following transient retinoblastoma-related protein silencing is revealed by microcomputed tomography imaging and leads to a system-level physiological response to the altered cell division pattern.
- Author
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Dorca-Fornell, Carmen, Pajor, Radoslaw, Lehmeier, Christoph, Pérez-Bueno, Marísa, Bauch, Marion, Sloan, Jen, Osborne, Colin, Rolfe, Stephen, Sturrock, Craig, Mooney, Sacha, and Fleming, Andrew
- Subjects
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ARABIDOPSIS thaliana , *RETINOBLASTOMA , *PLANT proteins , *GENE silencing , *COMPUTED tomography , *PLANT cells & tissues , *MESOPHYLL tissue , *PLANT physiology , *PHYSIOLOGY , *FUNGI - Abstract
The causal relationship between cell division and growth in plants is complex. Although altered expression of cell-cycle genes frequently leads to altered organ growth, there are many examples where manipulation of the division machinery leads to a limited outcome at the level of organ form, despite changes in constituent cell size. One possibility, which has been under-explored, is that altered division patterns resulting from manipulation of cell-cycle gene expression alter the physiology of the organ, and that this has an effect on growth. We performed a series of experiments on retinoblastoma-related protein ( RBR), a well characterized regulator of the cell cycle, to investigate the outcome of altered cell division on leaf physiology. Our approach involved combination of high-resolution micro CT imaging and physiological analysis with a transient gene induction system, providing a powerful approach for the study of developmental physiology. Our investigation identifies a new role for RBR in mesophyll differentiation that affects tissue porosity and the distribution of air space within the leaf. The data demonstrate the importance of RBR in early leaf development and the extent to which physiology adapts to modified cellular architecture resulting from altered cell-cycle gene expression. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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- View/download PDF
49. Genome-wide transcriptomic analysis of the sporophyte of the moss Physcomitrella patens.
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O’Donoghue, Martin-Timothy, Chater, Caspar, Wallace, Simon, Gray, Julie E., Beerling, David J., and Fleming, Andrew J.
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PHYSCOMITRELLA patens , *GENETIC transcription , *BRYOPHYTES , *HAPLOIDY , *GAMETOPHYTES , *GENE expression - Abstract
Bryophytes, the most basal of the extant land plants, diverged at least 450 million years ago. A major feature of these plants is the biphasic alternation of generations between a dominant haploid gametophyte and a minor diploid sporophyte phase. These dramatic differences in form and function occur in a constant genetic background, raising the question of whether the switch from gametophyte-to-sporophyte development reflects major changes in the spectrum of genes being expressed or alternatively whether only limited changes in gene expression occur and the differences in plant form are due to differences in how the gene products are put together. This study performed replicated microarray analyses of RNA from several thousand dissected and developmentally staged sporophytes of the moss Physcomitrella patens, allowing analysis of the transcriptomes of the sporophyte and early gametophyte, as well as the early stages of moss sporophyte development. The data indicate that more significant changes in transcript profile occur during the switch from gametophyte to sporophyte than recently reported, with over 12% of the entire transcriptome of P. patens being altered during this major developmental transition. Analysis of the types of genes contributing to these differences supports the view of the early sporophyte being energetically and nutritionally dependent on the gametophyte, provides a profile of homologues to genes involved in angiosperm stomatal development and physiology which suggests a deeply conserved mechanism of stomatal control, and identifies a novel series of transcription factors associated with moss sporophyte development. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
50. Inducible Repression of Multiple Expansin Genes Leads to Growth Suppression during Leaf Development1[C][W].
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Goh, Hoe-Han, Sloan, Jennifer, Dorca-Fornell, Carmen, and Fleming, Andrew
- Subjects
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EXTRACELLULAR matrix , *PLANT plasma membranes , *ARABIDOPSIS thaliana , *GENE expression , *GENE expression in plants , *PHYSIOLOGY ,LEAF growth - Abstract
Expansins are cell wall proteins implicated in the control of plant growth via loosening of the extracellular matrix. They are encoded by a large gene family, and data linked to loss of single gene function to support a role of expansins in leaf growth remain limited. Here, we provide a quantitative growth analysis of transgenics containing an inducible artificial microRNA construct designed to down-regulate the expression of a number of expansin genes that an expression analysis indicated are expressed during the development of Arabidopsis (Arabidopsis thaliana) leaf 6. The results support the hypothesis that expansins are required for leaf growth and show that decreased expansin gene expression leads to a more marked repression of growth during the later stage of leaf development. In addition, a histological analysis of leaves in which expansin gene expression was suppressed indicates that, despite smaller leaves, mean cell size was increased. These data provide functional evidence for a role of expansins in leaf growth, indicate the importance of tissue/organ developmental context for the outcome of altered expansin gene expression, and highlight the separation of the outcome of expansin gene expression at the cellular and organ levels. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
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