1. Comparison of hyperthermia and adrenaline to enhance the intratumoral accumulation of cisplatin in a murine model of peritoneal carcinomatosis
- Author
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Patrick Rat, Hervé Tixier, François Radais, Delphine Delroeux, François Ghiringhelli, Sylvain Ladoire, Olivier Facy, Pablo Ortega-Deballon, Bruno Chauffert, Lipides - Nutrition - Cancer (U866) (LNC), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA), Service de Chirurgie Digestive, Cancérologique, Générale, Endocrinienne et Urgences (CHU de Dijon), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Service de Chirurgie Digestive [CHRU Besançon], Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), This paper was supported by grants from the French National League against Cancer (Committees of Saône et Loire, Nièvre, and Côte d'Or)., BMC, Ed., Service de chirurgie digestive et cancérologie, Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand ( CHU Dijon ), Lipides - Nutrition - Cancer (U866) ( LNC ), Université de Bourgogne ( UB ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon ( ENSBANA ), Service de chirurgie digestive [Besançon], Centre Hospitalier Régional Universitaire [Besançon] ( CHRU Besançon ) -Hôpital Jean Minjoz, Service d'oncologie médicale, and CHU Amiens-Picardie
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MESH : Hyperthermia, Induced ,MESH : Rats, Inbred Strains ,Cancer Research ,Pathology ,Pharmacology ,[ SDV.CAN ] Life Sciences [q-bio]/Cancer ,Peritoneal Neoplasm ,0302 clinical medicine ,MESH : Tumor Cells, Cultured ,Tumor Cells, Cultured ,Medicine ,MESH: Peritoneal Neoplasms ,MESH : Female ,MESH: Animals ,Peritoneal Neoplasms ,Ovarian Neoplasms ,MESH : Cell Survival ,0303 health sciences ,MESH : Rats ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,MESH: Rats, Inbred Strains ,3. Good health ,MESH : Antineoplastic Agents ,MESH: Ovarian Neoplasms ,Epinephrine ,Oncology ,MESH: Cell Survival ,030220 oncology & carcinogenesis ,Female ,medicine.drug ,Hyperthermia ,medicine.medical_specialty ,MESH: Rats ,Cell Survival ,MESH: Epinephrine ,Antineoplastic Agents ,[SDV.CAN]Life Sciences [q-bio]/Cancer ,lcsh:RC254-282 ,MESH : Cisplatin ,03 medical and health sciences ,[SDV.CAN] Life Sciences [q-bio]/Cancer ,MESH : Peritoneal Neoplasms ,In vivo ,Animals ,Humans ,MESH: Tumor Cells, Cultured ,MESH: Hyperthermia, Induced ,030304 developmental biology ,Cisplatin ,MESH: Humans ,MESH : Ovarian Neoplasms ,business.industry ,Research ,MESH : Humans ,Rats, Inbred Strains ,Hyperthermia, Induced ,medicine.disease ,MESH : Disease Models, Animal ,Rats ,Disease Models, Animal ,MESH: Cisplatin ,Apoptosis ,MESH : Epinephrine ,Cancer cell ,MESH: Antineoplastic Agents ,MESH : Animals ,MESH: Disease Models, Animal ,business ,Ovarian cancer ,MESH: Female - Abstract
Background The best method to deliver intraperitoneal chemotherapy (IPC) for peritoneal carcinomatosis from ovarian cancer is not well defined. The aim of this study was to assess the ability of hyperthermia and adrenaline to enhance the intratumoral accumulation of cisplatin in a rat model of peritoneal carcinomatosis. Methods Four groups of 5 BDIX rats with ovarian peritoneal carcinomatosis underwent IPC with 30 mg/l of cisplatin according to the following conditions: normothermia at 37° for 1 or 2 hours, hyperthermia at 42°C for 1 hour or normothermia at 37°C for 2 hours with 2 mg/l adrenaline. Tissue platinum content was measured by atomic absorption spectroscopy. The effect of hyperthermia, adrenaline and the duration of exposure to the drug was measured in vivo (tissue concentration of platinum in tumor, abdominal and extra abdominal tissues) and in vitro (cytotoxicity on human ovarian cancer cells). Results In vitro, hyperthermia and longer exposure enhanced the accumulation and the cytotoxic effect of cisplatin on cancer cells. In vivo, only the 2 hours treatment with adrenaline resulted in increased platinum concentrations. The rats treated with adrenaline showed significantly lower concentrations of cisplatin in extra peritoneal tissues than those treated with hyperthermia. Conclusion Adrenaline is more effective than hyperthermia in order to enhance the intratumoral concentration of cisplatin in rats with peritoneal carcinomatosis from ovarian origin. It may also decrease the systemic absorption of the drug.
- Published
- 2011