12 results on '"Ansaldi, J."'
Search Results
2. PMP22 overexpression causes dysmyelination in mice
- Author
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Robaglia-Schlupp, A., Pizant, J., Norreel, J.-C., Passage, E., Sabéran-Djoneidi, D., Ansaldi, J.-L., Vinay, L., Figarella-Branger, D., Lévy, N., Clarac, F., Cau, P., Pellissier, J.-F., and Fontés, M.
- Published
- 2002
3. Oral Communication Abstracts
- Author
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Jumeau, C, Rupin, A, Villeneuve, N, Mougenot, N, Ansaldi, J, Alessi, Mc, Hatem, S, David-Dufilho, M, Unité de Recherche sur les Maladies Cardiovasculaires, du Métabolisme et de la Nutrition = Research Unit on Cardiovascular and Metabolic Diseases (ICAN), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut de Cardiométabolisme et Nutrition = Institute of Cardiometabolism and Nutrition [CHU Pitié Salpêtrière] (IHU ICAN), CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), SERVIER, Institut National de la Santé et de la Recherche Médicale (INSERM), Nutrition, obésité et risque thrombotique (NORT), Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), and Institut de Recherche International Servier - Grant number 2012/0374
- Subjects
Pharmacology ,business.industry ,[SDV]Life Sciences [q-bio] ,CCR3 ,030226 pharmacology & pharmacy ,Blockade ,03 medical and health sciences ,[SPI]Engineering Sciences [physics] ,0302 clinical medicine ,Medicine ,Pharmacology (medical) ,business ,030217 neurology & neurosurgery ,Collagen-induced arthritis - Published
- 2016
4. Direct thrombin inhibitors but not warfarin prevent the atrial dilation during heart failure in the rat
- Author
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Jumeau, C., Rupin, A., Villeneuve, N., Mougenot, N., Ansaldi, J., Marie-Christine Alessi, Hatem, S., Monique DAVID-DUFILHO, ProdInra, Migration, Institut National de la Santé et de la Recherche Médicale (INSERM), SERVIER, Nutrition, obésité et risque thrombotique (NORT), and Aix Marseille Université (AMU)-Institut National de la Recherche Agronomique (INRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)
- Subjects
[SDV] Life Sciences [q-bio] ,[SDV]Life Sciences [q-bio] ,ComputingMilieux_MISCELLANEOUS - Abstract
International audience
- Published
- 2016
5. Fish oil supplementation prevents diabetes-induced nerve conduction velocity and neuroanatomical changes in rats.
- Author
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Gerbi, Alain, Maixent, Jean-Michel, Gerbi, A, Maixent, J M, Ansaldi, J L, Pierlovisi, M, Coste, T, Pelissier, J F, Vague, P, and Raccah, D
- Subjects
FISH oils ,SCIATIC nerve ,PHYSIOLOGY ,ADENOSINE triphosphatase ,ANIMAL experimentation ,COMPARATIVE studies ,DIABETES ,DIETARY supplements ,RESEARCH methodology ,MEDICAL cooperation ,NEURAL conduction ,NEURONS ,RATS ,RESEARCH ,TIME ,EVALUATION research - Abstract
Diabetic neuropathy has been associated with a decrease in nerve conduction velocity, Na,K-ATPase activity and characteristic histological damage of the sciatic nerve. The aim of this study was to evaluate the potential effect of a dietary supplementation with fish oil [(n-3) fatty acids] on the sciatic nerve of diabetic rats. Diabetes was induced by intravenous streptozotocin injection. Diabetic animals (n = 20) were fed a nonpurified diet supplemented with either olive oil (DO) or fish oil (DM), and control animals (n = 10) were fed a nonpurified diet supplemented with olive oil at a daily dose of 0.5 g/kg by gavage for 8 wk. Nerves were characterized by their conduction velocity, morphometric analysis and membrane Na, K-ATPase activity. Nerve conduction velocity, as well as Na,K-ATPase activity, was improved by fish oil treatment. A correlation was found between these two variables (R = 0.999, P < 0.05). Moreover, a preventive effect of fish oil was observed on nerve histological damage [endoneurial edema, axonal degeneration (by 10-15%) with demyelination]. Moreover, the normal bimodal distribution of the internal diameter of myelinated fibers was absent in the DO group and was restored in the DM group. These data suggest that fish oil therapy may be effective in the prevention of diabetic neuropathy. [ABSTRACT FROM AUTHOR]
- Published
- 1999
- Full Text
- View/download PDF
6. Effects of castration and testosterone on Fel d I production by sebaceous glands of male cats: II--morphometric assessment.
- Author
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Charpin, C., Zielonka, T. M., Charpin, D., Ansaldi, J. -L., Allasia, C., and Vervloet, D.
- Subjects
TESTOSTERONE ,ANDROGENS ,CYTOPLASM ,CELL membranes ,EXOCRINE secretions ,CASTRATION - Abstract
A morphometric study of cat sebaceous glands was performed to evaluate the effects of castration and testosterone treatments. Skin biopsies were taken in six cats before castration, after castration and after the testosterone injections administered after castration (total number of biopsies: 18). Ninety 8/im thick sections of each biopsy were assessed for image analysis processing (SAMBA 2005, ALCATEL TITN). The variations in glands and cells size were evaluated on digitized microscopic images by morphometric parameters included in the SAMBA software package. An original software was developed for the analysis of the spacial gland structure. The best morphometric parameters were selected in a first step of the study, and included the nuclear surface (NS), the cell surface (CS) and the nuclear/cellular surface ratio (N/C). These three parameters were then compared in each group of samples for the six cats. It was shown that after castration the N/C (21%) significantly increased compared with prior to castration (12.6%). This 59.8% increase was mainly due to cell cytoplasm shrinking reflecting a decrease of the cell activity. The testosterone administered after castration produced a reverse effect with a N/C ratio back to normal (11.4%) and a significant cell cytoplasm and gland enlargement as shown by the three dimension constructions. This morphometric data correlated with the measurement of sebum and Fel d I productions. The negative effects of castration and the positive effects of testosterone on the sebaceous cells and glands volume favour the hypothesis that cat sebaceous cells are subject to hormonal control this is also likely to apply to the Fel d I production. [ABSTRACT FROM AUTHOR]
- Published
- 1994
- Full Text
- View/download PDF
7. Digitization of microcalcifications in breast radiographs. Correlation with pathologic data.
- Author
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Charpin C, Allasia C, Davies JD, Devictor B, Boulat J, Ansaldi JL, Lavaut MN, Serradour B, Andrac L, and Piana L
- Subjects
- Calcinosis diagnostic imaging, Carcinoma in Situ diagnostic imaging, Carcinoma in Situ pathology, Carcinoma, Intraductal, Noninfiltrating diagnostic imaging, Carcinoma, Intraductal, Noninfiltrating pathology, Carcinoma, Lobular diagnostic imaging, Carcinoma, Lobular pathology, Female, Humans, Mammography, Radiographic Image Enhancement methods, Sensitivity and Specificity, Breast Neoplasms diagnostic imaging, Breast Neoplasms pathology, Calcinosis pathology
- Abstract
A series of 104 nonpalpable breast lesions detected by mammograms and containing microcalcifications were studied. Intraoperative radiographs of intact and sliced specimens were assessed, followed by microscopic diagnosis on frozen sections of areas containing microcalcifications. Microcalcifications detected on mammograms and radiographs of the specimens were digitized and evaluated in accordance with morphometric parameters, including the mean surface, shape factor, bend energy, envelope surface and total surface, total number and concentration of microcalcifications. Benign disorders, atypical hyperplasia and carcinomas accounted for 47.2%, 4.8% and 48% of the tissue lesions, respectively, but the disorders were most often heterogeneous and mixed. Most, but not all, parameters significantly correlated with the three types of radiographs, although radiographs of the sliced specimens provided images of the best quality. Only two parameters, mean size and bend energy, were significantly different (P = .008, .0036) in benign and malignant lesions. It is concluded that image analysis of digitized microcalcifications in radiographs may provide quantitative data helpful in mammogram interpretation.
- Published
- 1995
8. Morphological polarization of human polymorphonuclear leucocytes in response to three different chemoattractants: an effector response independent of calcium rise and tyrosine kinases.
- Author
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Lepidi H, Zaffran Y, Ansaldi JL, Mege JL, and Capo C
- Subjects
- Actins blood, Actins chemistry, Actins drug effects, Adult, Cell Membrane drug effects, Cell Membrane physiology, Cell Membrane ultrastructure, Chelating Agents pharmacology, Complement C5a pharmacology, Cytosol metabolism, Egtazic Acid analogs & derivatives, Egtazic Acid pharmacology, Humans, In Vitro Techniques, Interleukin-8 pharmacology, Kinetics, Microscopy, Electron, Scanning, Neutrophils drug effects, Superoxides blood, Calcium blood, Chemotactic Factors pharmacology, N-Formylmethionine Leucyl-Phenylalanine pharmacology, Neutrophils cytology, Neutrophils physiology, Protein-Tyrosine Kinases blood
- Abstract
Chemoattractants such as interleukin-8, C5a and N-formylmethionyl-leucyl-phenylalanine induce a cytosolic calcium rise involved in triggering the secretory functions of human polymorphonuclear leucocytes. We studied the possible role of calcium rise in membrane ruffling, actin polymerization, filamentous actin distribution, and morphological polarization, which are all events contributing to chemotaxis. Membrane ruffling was assessed by right-angle light-scatter changes, the cellular content of polymerized actin by fluorescence of bodipy phallacidin, the intracellular distribution of filamentous actin by fluorescence microscopy and image digitization, and morphological polarization by scanning electron microscopy. Pretreatment of polymorphonuclear leucocytes with 50 microM BAPTA/AM, an intracellular calcium chelator, lowered the basal level in cell calcium and inhibited the transient calcium rise stimulated by 2 nM interleukin-8, 2 nM C5a, and 10 nM N-formylmethionyl-leucyl-phenylalanine. However, BAPTA pretreatment of polymorphonuclear leucocytes did not modify membrane ruffling, actin polymerization, filamentous actin distribution, and morphological polarization stimulated by chemoattractants. Downstream effectors may be protein tyrosine kinases. However, the tyrosine kinase inhibitor tyrphostin did not affect the cytoskeletal characteristics elicited by chemoattractants. Taken together, our results suggest that the transductional pathway leading to cytoskeleton organization and morphological polarization of polymorphonuclear leucocytes is different from that leading to secretion.
- Published
- 1995
- Full Text
- View/download PDF
9. Effects of castration and testosterone on Fel dI production by sebaceous glands of male cats: II--Morphometric assessment.
- Author
-
Charpin C, Zielonka TM, Charpin D, Ansaldi JL, Allasia C, and Vervloet D
- Subjects
- Animals, Cats, Male, Sebaceous Glands drug effects, Sebaceous Glands immunology, Sebum immunology, Skin drug effects, Skin immunology, Allergens biosynthesis, Castration, Glycoproteins biosynthesis, Sebaceous Glands pathology, Skin pathology, Testosterone pharmacology
- Abstract
A morphometric study of cat sebaceous glands was performed to evaluate the effects of castration and testosterone treatments. Skin biopsies were taken in six cats before castration, after castration and after the testosterone injections administered after castration (total number of biopsies: 18). Ninety 8 microns thick sections of each biopsy were assessed for image analysis processing (SAMBA 2005, ALCATEL TITN). The variations in glands and cells size were evaluated on digitized microscopic images by morphometric parameters included in the SAMBA software package. An original software was developed for the analysis of the spacial gland structure. The best morphometric parameters were selected in a first step of the study, and included the nuclear surface (NS), the cell surface (CS) and the nuclear/cellular surface ratio (N/C). These three parameters were then compared in each group of samples for the six cats. It was shown that after castration the N/C (21%) significantly increased compared with prior to castration (12.6%). This 59.8% increase was mainly due to cell cytoplasm shrinking reflecting a decrease of the cell activity. The testosterone administered after castration produced a reverse effect with a N/C ratio back to normal (11.4%) and a significant cell cytoplasm and gland enlargement as shown by the three dimension constructions. This morphometric data correlated with the measurement of sebum and Fel dI productions. The negative effects of castration and the positive effects of testosterone on the sebaceous cells and glands volume favour the hypothesis that cat sebaceous cells are subject to hormonal control this is also likely to apply to the Fel dI production.
- Published
- 1994
- Full Text
- View/download PDF
10. X-raying of sliced surgical specimens during surgery: an improvement of the histological diagnosis of impalpable breast lesions with microcalfications.
- Author
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Charpin C, Bonnier P, Habib MC, Andrac L, Vacheret H, Lavaut MN, Ansaldi JL, Allasia C, and Piana L
- Subjects
- Breast Neoplasms surgery, Calcinosis surgery, Carcinoma diagnostic imaging, Carcinoma pathology, Carcinoma surgery, Carcinoma in Situ diagnostic imaging, Carcinoma in Situ pathology, Carcinoma in Situ surgery, Carcinoma, Intraductal, Noninfiltrating diagnostic imaging, Carcinoma, Intraductal, Noninfiltrating pathology, Carcinoma, Intraductal, Noninfiltrating surgery, Female, Histological Techniques, Humans, Mammography, Neoplasm Invasiveness, Breast Neoplasms diagnostic imaging, Breast Neoplasms pathology, Calcinosis diagnostic imaging, Calcinosis pathology
- Abstract
Surgical specimens, from 78 patients operated for impalpable breast lesions containing microcalcifications detected on mammographs (breast cancer screening program) were systematically examined by a pathologist during the surgical intervention. The per-operative procedures encompassed (i) X-raying of the unfixed specimens to ascertain that the latter did enclose the screened lesion, (ii) slicing and subsequent X-raying of the specimens slices in order to locate the microcalcifications, (iii) histological evaluation of areas containing the microcalcifications on frozen sections. Thirty-two of the lesions were histologically benign, 55% were malignant and 13% borderline. Forty of the carcinomas were in situ and 60% were invasive. The per-operative histological diagnosis was correct in 65% of the case, erroneous in 10% and uncertain in 25%. Malignancy was never overscored. In 65% of the carcinomas diagnosed during the surgical intervention, the in sano margins excision and axillary lymph node removal could be performed (one stage surgical treatment). These results suggest that X-raying of sliced specimens and histological evaluation during the surgical intervention ascertains that lesions screened by mammographs are effectively and completely removed, and that they are precisely and extensively histologically examined after being identified and located.
- Published
- 1992
11. Daytime fluctuations of plasminogen activator inhibitor 1 (PAI-1) in populations with high PAI-1 levels.
- Author
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Juhan-Vague I, Alessi MC, Raccah D, Aillaud MF, Billerey M, Ansaldi J, Philip-Joet C, and Vague P
- Subjects
- Adult, Female, Fibrinolysis physiology, Humans, Infections blood, Inflammation blood, Insulin Resistance physiology, Male, Middle Aged, Obesity blood, Pregnancy, Circadian Rhythm physiology, Plasminogen Inactivators blood
- Abstract
The mechanism underlying diurnal variations in PAI-1 as well as the cellular origin of PAI-1 in subjects with high PAI-1 levels are unknown. We evaluated diurnal changes (8:00 am vs 4:00 pm) in PAI-1 (functional and immunological assays), t-PA Ag and t-PA/PAI-1 complex levels in controls and subjects with high PAI-1 levels. Three test groups were recruited among obese hyperinsulinemic subjects, emergency care unit patients with inflammatory syndrome or infection and pregnant women. The classical afternoon decrease of PAI-1 level was observed in controls and obese subjects but its amplitude was greater in the latter. The decrease in t-PA Ag and t-PA/PAI-1 complex levels was the same in controls and in obese. As, in previous studies, elevated PAI-1 levels have been correlated with insulin resistance and a decrease in insulin sensibility has been described in the early morning, it is proposed that this "dawn phenomenon" could be implicated in the circadian variations of PAI-1 in controls and could be amplified in obese subjects. Great variability in PAI-1, t-PA Ag or t-PA/PAI-1 complex levels was observed in patients with acute inflammatory syndrome or infection for whom classical biorhythms are suppressed. No diurnal changes in PAI-1 and other fibrinolytic parameters were observed in patients with inflammatory syndrome or in pregnant women suggesting that other sources and/or other regulatory mechanisms of PAI-1 production are involved.
- Published
- 1992
12. Deficient t-PA release and elevated PA inhibitor levels in patients with spontaneous or recurrent deep venous thrombosis.
- Author
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Juhan-Vague I, Valadier J, Alessi MC, Aillaud MF, Ansaldi J, Philip-Joet C, Holvoet P, Serradimigni A, and Collen D
- Subjects
- Adult, Aged, Female, Fibrinolysis, Humans, Male, Middle Aged, Plasminogen Inactivators, Serum Globulins analysis, Thrombophlebitis etiology, Tissue Plasminogen Activator antagonists & inhibitors, Triglycerides blood, Glycoproteins metabolism, Thrombophlebitis blood, Tissue Plasminogen Activator deficiency
- Abstract
The fibrinolytic system was investigated in 120 patients with spontaneous or recurrent deep vein thrombosis (DVT) without any known organic disease able to explain by itself the occurrence of a thrombosis and without any known defect of antithrombin III, Heparin Cofactor II, Protein C, or Protein S. The assays included: Euglobulin fibrinolytic activity (EFA), tissue-type plasminogen activator related antigen (t-PA-Ag) and plasminogen activator inhibitor activity (PA inhibitor), which were measured before and after 10 min of venous occlusion (V.O.). On the basis of the results, the patients could be classified in 3 groups: good responders with an at least two-fold increase of EFA after venous occlusion (n = 76), poor responders with a lesser increase of EFA due to deficient release of t-PA (n = 12), and poor responders with a normal t-PA release but an increased level of PA-Inhibitor (n = 32). The poor responders due to deficient t-PA release (10% of total) had a higher incidence of recurrence of deep vein thrombosis, than the other groups (p less than 0.01). An overall correlation was found between the level of PA-Inhibitor activity and the triglyceride level (r = 0.40, p less than 0.01), suggesting that these elevations may be due to a common cause, at least in some of the patients. It is concluded that a poor fibrinolytic response to venous occlusion occurs in 35 percent of DVT patients.(ABSTRACT TRUNCATED AT 250 WORDS)
- Published
- 1987
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