18 results on '"Buffoni, Leandro"'
Search Results
2. Evaluation of Th1/Th2, regulatory cytokines and transcriptional factor FoxP3 in sheep immunized with a partially protective and non-protective vaccine and challenged with Fasciola hepatica
- Author
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Ruiz-Campillo, María Teresa, Pacheco, Isabel Lourdes, Abril, Nieves, Bautista, María José, Martínez-Moreno, Álvaro, Martínez-Moreno, Francisco Javier, Buffoni, Leandro, Pérez, José, Molina-Hernández, Verónica, and Zafra, Rafael
- Published
- 2024
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- View/download PDF
3. Liver Histopathological and Immunohistochemical Evaluation from Fasciola hepatica Experimentally Infected and Reinfected Sheep.
- Author
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Herrera-Torres, Guillem, Ruiz-Campillo, María T., Bautista, María J., Martínez-Moreno, Francisco J., Zafra, Rafael, Buffoni, Leandro, Rufino-Moya, Pablo J., Martínez-Moreno, Álvaro, Molina-Hernández, Verónica, and Pérez, José
- Subjects
FASCIOLA hepatica ,SHEEP diseases ,SHEEP ,DRUG residues ,BILE ducts ,T cells ,HOST-parasite relationships - Abstract
Simple Summary: Fasciolosis is a parasitic disease of livestock causing important economic losses worldwide, and it is also a zoonosis. The treatment is based on the use of anthelmintic drugs, but the increase in resistance and the risk of drug residues in food make this approach no longer sustainable. Developing protective vaccines for the control of fasciolosis is postulated as an appropriate treatment, but a better knowledge of the host–parasite interaction is needed. The aim of the present study was to evaluate the hepatic lesions in sheep infected and reinfected with Fasciola hepatica during the acute and chronic stages of infection and the characterization of the hepatic inflammatory infiltrates using immunohistochemistry with CD3, Foxp3, iNOS, and CD163 antibodies. The most remarkable histopathological finding was the presence of large necrotic foci and/or hemorrhages adjacent to enlarged bile ducts containing adult parasites, suggesting flukes may have caused these lesions while feeding. In the literature, necrotic foci/hemorrhages are considered a consequence of parasite migration. In both the primoinfected and reinfected groups, and during acute and chronic stages of the infection, an increase in Foxp3 T cells with respect to uninfected controls and a poor expression of iNOS was found accompanied by a strong expression of CD163, suggesting a marked M2 activation of macrophages in the hepatic lesions. Fasciolosis is an important economic disease of livestock. There is a global interest in the development of protective vaccines since the current anthelmintic therapy is no longer sustainable. A better knowledge of the host–parasite interaction is needed to design effective vaccines. To date, few studies have evaluated host–parasite interaction by comparing infected and reinfected animals. The present study evaluates the microscopical hepatic lesions in sheep infected and reinfected with Fasciola hepatica during the acute and chronic stages of infection. The histopathological study revealed the presence of necrotizing foci (NF1) associated with larvae migration during the early stages of infection in the primoinfected (PI) and reinfected (RI) groups. In the late stages of infection of the PI group and at the early and late stages of infection in the RI groups, extensive necrotizing/hemorrhagic foci (NF2) were found in the vicinity of enlarged bile ducts, some containing adult flukes, suggesting parasites may have caused NF2 while feeding. The immunohistochemical study revealed an increase in Foxp3+ T cells in both PI and RI groups with respect to the UC group and in the infiltrates adjacent to NF1 in the RI groups with respect to the PI group, suggesting the F. hepatica induce Foxp3 T cell expansion to facilitate parasite survival. In addition, in both the PI and RI groups, and during acute and chronic stages of the infection, a poor expression of iNOS was found accompanied by a strong expression of CD163, suggesting a marked M2 activation of macrophages in the hepatic lesions, which may be related with healing processes, and it also may facilitate parasite survival. The main differences between PI and RI animals were the more severe infiltration of eosinophils and Foxp3+ T cells, whereas RI did not modify M2 activation of macrophages which occurs since the early stages of primoinfection. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
4. Serosurveillance of Trichinella sp. in wild boar and Iberian domestic suids in Mediterranean ecosystems of southwestern Spain.
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Buffoni, Leandro, Cano‐Terriza, David, Jiménez‐Martín, Débora, Jiménez‐Ruiz, Saúl, Martínez‐Moreno, Álvaro, Martínez‐Moreno, Francisco Javier, Zafra, Rafael, Pérez‐Caballero, Raúl, Risalde, María Ángeles, Gómez‐Guillamón, Félix, and García‐Bocanegra, Ignacio
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WILD boar , *TRICHINELLA , *SWINE , *SEROPREVALENCE , *SEROCONVERSION , *RISK exposure - Abstract
Aims: A cross‐sectional study was carried out to assess the seroprevalence and risk factors associated with Trichinella spp. exposure in wild boar and Iberian domestic pigs from Mediterranean ecosystems of southwestern Spain. Methods and Results: Serum samples from 1360 wild boar and 439 Iberian domestic pigs were obtained during 2015–2020, from regions where Iberian pigs are raised under extensive conditions, hence sharing habitat with wild boar. Seropositivity was found in 7.4% (100/1360; 95% CI: 6.1–8.9) of the wild boar analysed. In this species, the individual seroprevalence ranged from 3.6% (8/223) (hunting season 2016–2017) to 11.4% (37/326) (2018–2019). A significant higher seropositivity was observed during the hunting season 2018–2019 (p < 0.009: OR = 3.07; 95% CI = 1.32–7.18) and one statistically significant cluster was detected within the studied area, in south central Andalusia [Relative Risk (RR) = 2.9; p = 0.037]. Females showed a significantly higher seroprevalence than males (8.7% vs. 5.8%) (p < 0.001: OR = 1.58; 95% CI = 1.08–2.32). No seropositivity to Trichinella spp. was detected in Iberian domestic pigs (0.0%; 95% CI: 0.0–0.9). Conclusions: Although wild boar play an important role as a reservoir of Trichinella sp. in the Mediterranean ecosystems of southwestern Spain, our results suggest that the wild boar production system does not seem to pose a risk of Trichinella exposure to domestic pigs, despite sharing habitats in these ecosystems. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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5. Efficacy of a multivalent vaccine against Fasciola hepatica infection in sheep
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Zafra, Rafael, Buffoni, Leandro, Pérez-Caballero, Raúl, Molina-Hernández, Verónica, Ruiz-Campillo, María T., Pérez, José, Martínez-Moreno, Álvaro, and Martínez Moreno, Francisco J.
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- 2021
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6. Identification of protective peptides of Fasciola hepatica-derived cathepsin L1 (FhCL1) in vaccinated sheep by a linear B-cell epitope mapping approach
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Buffoni, Leandro, Garza-Cuartero, Laura, Pérez-Caballero, Raúl, Zafra, Rafael, Javier Martínez-Moreno, F., Molina-Hernández, Verónica, Pérez, José, Martínez-Moreno, Álvaro, and Mulcahy, Grace
- Published
- 2020
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7. Characterization of dendritic cells and follicular dendritic cells in the hepatic lymph nodes and liver of sheep experimentally infected with Fasciola hepatica
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Ruiz-Campillo, María Teresa, Molina-Hernández, Verónica, Bautista, María José, Pacheco, Isabel L., Zafra, Rafael, Buffoni, Leandro, Martínez-Moreno, Francisco Javier, Martínez-Moreno, Alvaro, and Pérez, José
- Published
- 2020
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8. A Quality Assurance Discrimination Tool for the Evaluation of Satellite Laboratory Practice Excellence in the Context of the European Official Meat Inspection for Trichinella spp.
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Villegas-Pérez, José, Navas-González, Francisco Javier, Serrano, Salud, García-Viejo, Fernando, and Buffoni, Leandro
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MEAT inspection ,TRICHINELLA ,QUALITY assurance ,FOODBORNE diseases ,ZOONOSES - Abstract
Trichinellosis is a parasitic foodborne zoonotic disease transmitted by ingestion of raw or undercooked meat containing the first larval stage (L1) of the nematode. To ensure the quality and safety of food intended for human consumption, meat inspection for detection of Trichinella spp. larvae is a mandatory procedure according to EU regulations. The implementation of quality assurance practices in laboratories that are responsible for Trichinella spp. detection is essential given that the detection of this parasite is still a pivotal threat to public health, and it is included in list A of Annex I, Directive 2003/99/EC, which determines the agents to be monitored on a mandatory basis. A Quality Management System (QMS) was applied to slaughterhouses and game handling establishments conducting Trichinella spp. testing without official accreditation but under the supervision of the relevant authority. This study aims to retrospectively analyze the outcomes of implementing the QMS in slaughterhouses and game handling establishments involved in Trichinella testing in southern Spain. Canonical discriminant analyses (CDAs) were performed to design a tool enabling the classification of SLs while determining whether linear combinations of measures of quality-assurance-related traits describe within- and between-SL clustering patterns. The participation of two or more auditors improves the homogeneity of the results deriving from audits. However, when training expertise ensures that such levels of inter-/intralaboratory homogeneity are reached, auditors can perform single audits and act as potential trainers for other auditors. Additionally, technical procedure issues were the primary risk factors identified during audits, which suggests that they should be considered a critical control point within the QMS. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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9. Expression of free radicals by peritoneal cells of sheep during the early stages of Fasciola hepatica infection
- Author
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Pérez-Caballero, Raúl, Buffoni, Leandro, Martínez-Moreno, F. Javier, Zafra, Rafael, Molina-Hernández, Verónica, Pérez, José, and Martínez-Moreno, Álvaro
- Published
- 2018
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10. Antigen-specific response of CD4+ T cells and hepatic lymph node cells to Fasciola hepatica-derived molecules at the early and late stage of the infection in sheep.
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Pérez-Caballero, Raúl, Martínez-Moreno, F. Javier, Corripio-Miyar, Yolanda, McNeilly, Tom N., Cwiklinski, Krystyna, Dalton, John P., Zafra, Rafael, Pérez, José, Martínez-Moreno, Álvaro, and Buffoni, Leandro
- Abstract
The immunomodulatory capacity of F. hepatica antigens is probably one of the main reasons for the development of a driven non-protective Th2 immune response. In this study, we analysed the cellular response of hepatic lymph node cells and CD4
+ T cells in terms of proliferative response, efficiency of antigen presentation and cytokine production, to F. hepatica-derived molecules, at early and late stages of the infection. Thirty-one sheep were allocated into five groups and were slaughtered at 16 dpi and 23 wpi. In order to analyse antigen-specific response, the following F. hepatica recombinant molecules were used: rFhCL1, rFhCL2, rFhCL3, rFhCB1, rFhCB2, rFhCB3, rFhStf-1, rFhStf-2, rFhStf-3 and rFhKT1. A cell proliferation assay using hepatic lymph node cells and an antigen presentation cell assay using CD4+ T cells were performed. At 16 dpi, all molecules but rFhStf-2 and rFhKT1 elicited a significant cell proliferative response on hepatic lymph node cells of infected animals. At both early and late stage of the infection, antigen presentation of rFhCB3 and rFhCL2 resulted in higher stimulation index of CD4+ T cells which was IL-2 mediated, although no statistically significant when compared to uninfected animals. Significant cytokine production (IL-4, IL-10 and IFN-γ) was conditioned by the antigen-specific cell stimulation. No CD4+ T cell exhaustion was detected in infected sheep at the chronic stage of the infection. This study addressed antigen-specific response to F. hepatica-derived molecules that are involved in key aspects of the parasite survival within the host. [ABSTRACT FROM AUTHOR]- Published
- 2021
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11. Acuaroid nematodes in the common kestrel ( Falco tinnunculus) in the south of Spain
- Author
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Acosta, Isabel, Hernández, Santiago, Gutiérrez, Pedro N., Martínez-Cruz, María S., Hernández, Esther, Buffoni, Leandro, and Javier Martínez-Moreno, F.
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- 2010
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12. The Sigma Class Glutathione Transferase from the Liver Fluke Fasciola hepatica.
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LaCourse, E. James, Perally, Samirah, Morphew, Russell M., Moxon, Joseph V., Prescott, Mark, Dowling, David J., O'Neill, Sandra M., Kipar, Anja, Hetzel, Udo, Hoey, Elizabeth, Zafra, Rafael, Buffoni, Leandro, Pérez Arévalo, José, and Brophy, Peter M.
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LIVER flukes ,GLUTATHIONE transferase ,FASCIOLA hepatica ,CYCLOOXYGENASES ,VACCINE trials - Abstract
Background: Liver fluke infection of livestock causes economic losses of over US$ 3 billion worldwide per annum. The disease is increasing in livestock worldwide and is a re-emerging human disease. There are currently no commercial vaccines, and only one drug with significant efficacy against adult worms and juveniles. A liver fluke vaccine is deemed essential as short-lived chemotherapy, which is prone to resistance, is an unsustainable option in both developed and developing countries. Protein superfamilies have provided a number of leading liver fluke vaccine candidates. A new form of glutathione transferase (GST) family, Sigma class GST, closely related to a leading Schistosome vaccine candidate (Sm28), has previously been revealed by proteomics in the liver fluke but not functionally characterised. Methodology/Principal Findings: In this manuscript we show that a purified recombinant form of the F. hepatica Sigma class GST possesses prostaglandin synthase activity and influences activity of host immune cells. Immunocytochemistry and western blotting have shown the protein is present near the surface of the fluke and expressed in eggs and newly excysted juveniles, and present in the excretory/secretory fraction of adults. We have assessed the potential to use F. hepatica Sigma class GST as a vaccine in a goat-based vaccine trial. No significant reduction of worm burden was found but we show significant reduction in the pathology normally associated with liver fluke infection. Conclusions/Significance: We have shown that F. hepatica Sigma class GST has likely multi-functional roles in the host-parasite interaction from general detoxification and bile acid sequestration to PGD synthase activity. Author Summary: Combating neglected parasitic diseases is of paramount importance to improve the health of human populations and/or their domestic animals. Uncovering key roles in host-parasite interactions may support the vaccine potential portfolio of a parasite protein. Fasciola hepatica causes global disease in humans and their livestock but no commercial vaccines are available. Members of the Sigma class glutathione transferase (GST) family have long been highlighted as vaccine candidates towards parasitic flatworms. To this end, a Sigma class GST is currently undergoing phase II clinical trials to protect against infection from the schistosomes. In this study we characterise the protein from F. hepatica following four work pathways that 1) confirm its designation as a Sigma class GST using substrate profiling, 2) assess prostaglandin synthase activity and its effect on host immune cells, 3) localise the Sigma GST within adult fluke and between ontogenic stages and 4) measure its potential as a vaccine candidate. The work presented here shows F. hepatica Sigma class GST to have key host-parasite roles and we suggest, warrants further investigation for inclusion into vaccine formulations. [ABSTRACT FROM AUTHOR]
- Published
- 2012
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13. A Partially Protective Vaccine for Fasciola hepatica Induced Degeneration of Adult Flukes Associated to a Severe Granulomatous Reaction in Sheep.
- Author
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Molina-Hernández, Verónica, Ruiz-Campillo, María T., Martínez-Moreno, Francisco J., Buffoni, Leandro, Martínez-Moreno, Álvaro, Zafra, Rafael, Bautista, María J., Escamilla, Alejandro, Pérez-Caballero, Raúl, and Pérez, José
- Subjects
FASCIOLA hepatica ,VACCINE trials ,VACCINE effectiveness ,MULTINUCLEATED giant cells ,DRUG residues ,SHEEP ,CELL death ,BILE - Abstract
Simple Summary: Fasciolosis is a parasitic disease of livestock causing important economic losses worldwide and it is also a zoonosis. Current therapy relies on the use of anthelmintic drugs, which is no longer sustainable due to the increase of anthelmintic resistance and the risk of drug residues in food. A deep understanding of the host-parasite interaction is required to develop protective vaccines for the control of fasciolosis. The aim of the present study is to evaluate the hepatic lesions in sheep vaccinated with a partly protective vaccine for F. hepatica, a non-protective vaccine and an infected control group. The protective vaccine showed less severe hepatic lesions than the infected control group. In addition, in the protective vaccine group dead flukes surrounded by a severe granulomatous inflammation were observed, which taken together with the lower fluke burden, suggests that the host response induced by the partially protective vaccine may have been involved in the death of adult flukes of F. hepatica. This is the first study reporting the presence of degenerated flukes associated to a severe granulomatous inflammation in bile ducts in a vaccine trial, a finding that would be useful for improving vaccine efficacy in future trials. Fasciolosis is an important economic disease of livestock. There is a global interest in the development of protective vaccines since current anthelmintic therapy is no longer sustainable. A better knowledge of the host-parasite interaction is needed for the design of effective vaccines. The present study evaluates the microscopical hepatic lesions in sheep immunized with a partially protective vaccine (VAC1), a non-protective vaccine (VAC2), and an infected control group (IC). The nature of granulomatous inflammation associated with degeneration of adult flukes found in the VAC1 group was characterized by immunohistochemistry. Hepatic lesions (fibrous perihepatitis, chronic tracts, bile duct hyperplasia, infiltration of eosinophils and lymphocytes and plasma cells) were significantly less severe in the VAC1 group than in the IC group. Dead adult flukes within bile ducts were observed only in the VAC1 group and were surrounded by a severe granulomatous inflammation composed by macrophages and multinucleate giant cells with a high expression of lysozyme, CD163 and S100 markers, and a low expression of CD68. Numerous CD3+ T lymphocytes and scarce infiltrate of FoxP3+ Treg and CD208+ dendritic cells were present. This is the first report describing degenerated flukes associated to a severe granulomatous inflammation in bile ducts in a F. hepatica vaccine trial. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
14. Comparative dynamics of peritoneal cell immunophenotypes in sheep during the early and late stages of the infection with Fasciola hepatica by flow cytometric analysis.
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Pérez-Caballero, Raúl, Javier Martínez-Moreno, F., Zafra, Rafael, Molina-Hernández, Verónica, Pacheco, Isabel L., Teresa Ruiz-Campillo, M., Escamilla, Alejandro, Pérez, José, Martínez-Moreno, Álvaro, and Buffoni, Leandro
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FASCIOLA hepatica ,CYTOMETRY ,PERITONEUM ,TREMATODA ,APOPTOSIS - Abstract
Background: The peritoneal cell populations (PCP) are thought to play a crucial role during the early immune response in Fasciola hepatica infection while newly excysted juveniles (NEJ) are migrating in the peritoneal cavity (PC) towards the liver. In this study, we aimed to determine the immunophenotypes of the PCP and to analyse the dynamics of the recruitment of the PCP during the early and late stage of the infection in sheep infected with F. hepatica. Methods: Thirty-seven sheep were divided into three groups: Group 1 (n = 20) and 2 (n = 10) were challenged with F. hepatica, Group 3 (n = 7) was not infected and remained as uninfected control (UC). After the slaughtering, peritoneal lavages were carried out to isolate peritoneal cell populations at 1, 3, 9 and 18 days post-infection (dpi) for Group 1 and at 14 weeks post-infection (wpi) for Group 2 and 3. Flow cytometry was conducted to assess the dynamics of peritoneal cavity cell populations. Results: TCD4 cells showed a significant decrease at 1 and 18 dpi when compared to UC; no statistical differences were detected for TCD8 and WC1
+ γδ during the early stage of the infection with respect to the UC. CD14 cells exhibited a decreasing trend, with a significant decrease at 9 and 18 dpi when compared to the UC. The dynamics of MHCII and CD83 cells showed a similar increasing pattern from 3 to 18 dpi. During the chronic stage, both TCD4 and TCD8 cells showed no significant differences when compared to the UC, although a slight but statistically significant higher level of WC1+ γδ cells was observed. A lower percentage of antigen-presenting cells (APCs) was detected with respect to the UC. Conclusions: The recruitment of the lymphocytes subsets did not show a significant increase during the course of the infection and only WC1+ γδ cells displayed a significant increase at the chronic stage. For the CD14, a decreasing trend was observed during the early stage, which was statistically significant at the chronic stage of the infection. Peritoneal CD83 and MHCII cells developed an increasing trend during the early stage of infection, and showed a significant decrease at the late stage of the infection. [ABSTRACT FROM AUTHOR]- Published
- 2018
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15. Evaluation of hepatic changes and local and systemic immune responses in goats immunized with recombinant Peroxiredoxin (Prx) and challenged with Fasciola hepatica
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Mendes, Ricardo E., Pérez-Écija, Rafael A., Zafra, Rafael, Buffoni, Leandro, Martínez-Moreno, Álvaro, Dalton, John P., Mulcahy, Grace, and Pérez, José
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IMMUNE response , *IMMUNIZATION , *GOATS , *PHYSIOLOGY , *RECOMBINANT proteins , *FASCIOLA hepatica , *IMMUNOLOGICAL adjuvants , *MORPHOMETRICS , *IMMUNOHISTOCHEMISTRY , *IMMUNOGLOBULIN G , *LIVER injuries - Abstract
Abstract: Protection against Fasciola hepatica in goats immunized with Peroxiredoxin (Prx) was assessed. The experimental trial consisted of three groups of seven animals; group 1 were unimmunized and uninfected, group 2 were immunized with adjuvant only and group 3 were immunized with recombinant Prx in adjuvant (immunized and infected). Immunization with Prx in Quil A adjuvant, group 3, induced a reduction in fluke burden of 33.04% when compared to adjuvant control, group 2, although this difference was not significant. The hepatic gross and microscopical morphometric study revealed lower damage in the Prx-immunized compared to group 2 (p <0.05). Furthermore, immunohistochemical studies revealed that the Prx-immunized group exhibited reduced infiltration of CD4+, CD8+, IFN-γ+ and TCR+ (p <0.05); and CD2+ and IL-4+ (p <0.001) in hepatic lesions. Levels of anti-Prx serum IgG in group 3 showed a significant increase at the 4th week after challenge infection compared with group 2 (p <0.0001). This is the first report of ruminant immunization with recombinant Prx of F. hepatica. The study shows that this vaccine significantly reduces hepatic damage and encourages further studies to improve the vaccine efficacy. [Copyright &y& Elsevier]
- Published
- 2010
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16. Fasciolosis: pathogenesis, host-parasite interactions, and implication in vaccine development.
- Author
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Flores-Velázquez LM, Ruiz-Campillo MT, Herrera-Torres G, Martínez-Moreno Á, Martínez-Moreno FJ, Zafra R, Buffoni L, Rufino-Moya PJ, Molina-Hernández V, and Pérez J
- Abstract
Fasciola hepatica is distributed worldwide, causing substantial economic losses in the animal husbandry industry. Human fasciolosis is an emerging zoonosis in Andean America, Asia, and Africa. The control of the disease, both in humans and animals, is based on using anthelmintic drugs, which has resulted in increased resistance to the most effective anthelmintics, such as triclabendazole, in many countries. This, together with the concerns about drug residues in food and the environment, has increased the interest in preventive measures such as a vaccine to help control the disease in endemic areas. Despite important efforts over the past two decades and the work carried out with numerous vaccine candidates, none of them has demonstrated consistent and reproducible protection in target species. This is at least in part due to the high immunomodulation capacity of the parasite, making ineffective the host response in susceptible species such as ruminants. It is widely accepted that a deeper knowledge of the host-parasite interactions is needed for a more rational design of vaccine candidates. In recent years, the use of emerging technologies has notably increased the amount of data about these interactions. In the present study, current knowledge of host-parasite interactions and their implication in Fasciola hepatica vaccine development is reviewed., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Flores-Velázquez, Ruiz-Campillo, Herrera-Torres, Martínez-Moreno, Martínez-Moreno, Zafra, Buffoni, Rufino-Moya, Molina-Hernández and Pérez.)
- Published
- 2023
- Full Text
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17. Antigen-specific response of CD4 + T cells and hepatic lymph node cells to Fasciola hepatica-derived molecules at the early and late stage of the infection in sheep.
- Author
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Pérez-Caballero R, Martínez-Moreno FJ, Corripio-Miyar Y, McNeilly TN, Cwiklinski K, Dalton JP, Zafra R, Pérez J, Martínez-Moreno Á, and Buffoni L
- Subjects
- Animals, Fasciola hepatica physiology, Fascioliasis immunology, Fascioliasis parasitology, Liver immunology, Male, Sheep, Sheep Diseases parasitology, Sheep, Domestic, Antigens, Helminth immunology, Fascioliasis veterinary, Lymph Nodes immunology, Sheep Diseases immunology, T-Lymphocytes immunology
- Abstract
The immunomodulatory capacity of F. hepatica antigens is probably one of the main reasons for the development of a driven non-protective Th2 immune response. In this study, we analysed the cellular response of hepatic lymph node cells and CD4
+ T cells in terms of proliferative response, efficiency of antigen presentation and cytokine production, to F. hepatica-derived molecules, at early and late stages of the infection. Thirty-one sheep were allocated into five groups and were slaughtered at 16 dpi and 23 wpi. In order to analyse antigen-specific response, the following F. hepatica recombinant molecules were used: rFhCL1, rFhCL2, rFhCL3, rFhCB1, rFhCB2, rFhCB3, rFhStf-1, rFhStf-2, rFhStf-3 and rFhKT1. A cell proliferation assay using hepatic lymph node cells and an antigen presentation cell assay using CD4+ T cells were performed. At 16 dpi, all molecules but rFhStf-2 and rFhKT1 elicited a significant cell proliferative response on hepatic lymph node cells of infected animals. At both early and late stage of the infection, antigen presentation of rFhCB3 and rFhCL2 resulted in higher stimulation index of CD4+ T cells which was IL-2 mediated, although no statistically significant when compared to uninfected animals. Significant cytokine production (IL-4, IL-10 and IFN-γ) was conditioned by the antigen-specific cell stimulation. No CD4+ T cell exhaustion was detected in infected sheep at the chronic stage of the infection. This study addressed antigen-specific response to F. hepatica-derived molecules that are involved in key aspects of the parasite survival within the host.- Published
- 2021
- Full Text
- View/download PDF
18. Evaluation of local immune response to Fasciola hepatica experimental infection in the liver and hepatic lymph nodes of goats immunized with Sm14 vaccine antigen.
- Author
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Mendes RE, Zafra R, Pérez-Ecija RA, Buffoni L, Martínez-Moreno A, Tendler M, and Pérez J
- Subjects
- Animals, Fascioliasis prevention & control, Fatty Acid Transport Proteins administration & dosage, Goat Diseases immunology, Goats, Helminth Proteins administration & dosage, Liver immunology, Liver parasitology, Lymph Nodes immunology, Lymph Nodes parasitology, Vaccines immunology, Antigens, Helminth immunology, Fasciola hepatica immunology, Fascioliasis immunology, Fatty Acid Transport Proteins immunology, Goat Diseases parasitology, Helminth Proteins immunology
- Abstract
Protection against Fasciola hepatica in goats immunized with a synthetic recombinant antigen from Schistosoma mansoni fatty acid-binding protein 14 (rSm14) was investigated by assessing worm burdens, serum levels of hepatic enzymes, faecal egg count and hepatic damage, which was evaluated using gross and microscopic morphometric observation. The nature of the local immune response was assessed by examining the distribution of CD2+, CD4+, CD8+ and γ´+ T lymphocytes along with IgG+, IL-4+ and IFN-γ+ cells in the liver and hepatic lymph nodes (HLN). The goats used consisted of group 1 (unimmunized and uninfected), group 2 [infected control - immunized with Quillaia A (Quil A)] and group 3 (immunized with rSm14 in Quil A and infected), each containing seven animals. Immunization with rSm14 in Quil A adjuvant induced a reduction in gross hepatic lesions of 56.6% (p < 0.001) and reduced hepatic and HLN infiltration of CD2+, CD4+, CD8+ and γ´+ T lymphocytes as well as IL-4+ and IFN-γ+ cells (p < 0.05). This is the first report of caprine immunization against F. hepatica using a complete rSm14 molecule derived from S. mansoni. Immunization reduced hepatic damage and local inflammatory infiltration into the liver and HLN. However, considering that Quil A is not the preferential/first choice adjuvant for Sm14 immunization, further studies will be undertaken using the monophosphoryl lipid A-based family of adjuvants during clinical trials to facilitate anti-Fasciolavaccine development.
- Published
- 2010
- Full Text
- View/download PDF
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