41 results on '"Esplugues, Enric"'
Search Results
2. Dynamic metabolism of endothelial triglycerides protects against atherosclerosis in mice
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Boutagy, Nabil E., Gamez-Mendez, Ana, Fowler, Joseph W.M., Zhang, Hanming, Chaube, Bal K., Esplugues, Enric, Kuo, Andrew, Lee, Sungwoon, Horikami, Daiki, Zhang, Jiasheng, Citrin, Kathryn M., Singh, Abhishek K., Coon, Brian G., Lee, Monica Y., Suarez, Yajaira, Fernandez-Hernando, Carlos, and Sessa, William C.
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Lipids -- Physiological aspects ,Chronic diseases -- Physiological aspects ,Endothelium -- Physiological aspects ,Antilipemic agents -- Physiological aspects ,Lipase -- Physiological aspects ,Nitric oxide -- Physiological aspects ,Blood circulation disorders -- Physiological aspects ,Atherosclerosis -- Physiological aspects ,Health care industry - Abstract
Blood vessels are continually exposed to circulating lipids, and elevation of ApoB-containing lipoproteins causes atherosclerosis. Lipoprotein metabolism is highly regulated by lipolysis, largely at the level of the capillary endothelium lining metabolically active tissues. How large blood vessels, the site of atherosclerotic vascular disease, regulate the flux of fatty acids (FAs) into triglyceride-rich (TG-rich) lipid droplets (LDs) is not known. In this study, we showed that deletion of the enzyme adipose TG lipase (ATGL) in the endothelium led to neutral lipid accumulation in vessels and impaired endothelialdependent vascular tone and nitric oxide synthesis to promote endothelial dysfunction. Mechanistically, the loss of ATGL led to endoplasmic reticulum stress-induced inflammation in the endothelium. Consistent with this mechanism, deletion of endothelial ATGL markedly increased lesion size in a model of atherosclerosis. Together, these data demonstrate that the dynamics of FA flux through LD affects endothelial cell homeostasis and consequently large vessel function during normal physiology and in a chronic disease state., Introduction The vascular endothelium is essential in lipid partitioning (1). Specifically, in metabolically active tissues (e.g., heart, skeletal muscle, adipose), triglyceride-rich (TG-rich) lipoproteins (chylomicrons, VLDL) are metabolized by lipoprotein lipase [...]
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- 2024
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3. Intestinal type 1 regulatory T cells migrate to periphery to suppress diabetogenic T cells and prevent diabetes development
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Yu, Hua, Gagliani, Nicola, Ishigame, Harumichi, Huber, Samuel, Zhu, Shu, Esplugues, Enric, Herold, Kevan C., Wen, Li, and Flavell, Richard A.
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- 2017
4. The induction and function of the anti-inflammatory fate of TH17 cells
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Xu, Hao, Agalioti, Theodora, Zhao, Jun, Steglich, Babett, Wahib, Ramez, Vesely, Maria Carolina Amezcua, Bielecki, Piotr, Bailis, Will, Jackson, Ruaidhri, Perez, Daniel, Izbicki, Jakob, Licona-Limón, Paula, Kaartinen, Vesa, Geginat, Jens, Esplugues, Enric, Tolosa, Eva, Huber, Samuel, Flavell, Richard A., and Gagliani, Nicola
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- 2020
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5. Microbiota-driven interleukin-17-producing cells and eosinophils synergize to accelerate multiple myeloma progression
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Calcinotto, Arianna, Brevi, Arianna, Chesi, Marta, Ferrarese, Roberto, Garcia Perez, Laura, Grioni, Matteo, Kumar, Shaji, Garbitt, Victoria M., Sharik, Meaghen E., Henderson, Kimberly J., Tonon, Giovanni, Tomura, Michio, Miwa, Yoshihiro, Esplugues, Enric, Flavell, Richard A., Huber, Samuel, Canducci, Filippo, Rajkumar, Vincent S., Bergsagel, P. Leif, and Bellone, Matteo
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- 2018
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6. TFH cells progressively differentiate to regulate the germinal center response
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Weinstein, Jason S, Herman, Edward I, Lainez, Begoña, Licona-Limón, Paula, Esplugues, Enric, Flavell, Richard, and Craft, Joe
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- 2016
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7. Apoptosis in response to microbial infection induces autoreactive TH17 cells
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Campisi, Laura, Barbet, Gaetan, Ding, Yi, Esplugues, Enric, Flavell, Richard A, and Blander, J Magarian
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- 2016
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8. TH17 cells transdifferentiate into regulatory T cells during resolution of inflammation
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Gagliani, Nicola, Vesely, Maria Carolina Amezcua, Iseppon, Andrea, Brockmann, Leonie, Xu, Hao, Palm, Noah W., de Zoete, Marcel R., Licona-Limon, Paula, Paiva, Ricardo S., Ching, Travers, Weaver, Casey, Zi, Xiaoyuan, Pan, Xinghua, Fan, Rong, Garmire, Lana X., Cotton, Matthew J., Drier, Yotam, Bernstein, Bradley, Geginat, Jens, Stockinger, Brigitta, Esplugues, Enric, Huber, Samuel, and Flavell, Richard A.
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T cells -- Physiological aspects ,Inflammation -- Research ,Environmental issues ,Science and technology ,Zoology and wildlife conservation - Abstract
Inflammation is a beneficial host response to infection but can contribute to inflammatory disease if unregulated. The TH17 lineage of T helper (Th) cells can cause severe human inflammatory diseases. These cells exhibit both instability (they can cease to express their signature cytokine, IL-17A) (1) and plasticity (they can start expressing cytokines typical of other lineages) (1,2) upon in vitro re-stimulation. However, technical limitations have prevented the transcriptional profiling of pre- and post-conversion TH17 cells ex vivo during immune responses. Thus, it is unknown whether TH17 cell plasticity merely reflects change in expression of a few cytokines, or if TH17 cells physiologically undergo global genetic reprogramming driving their conversion from one T helper cell type to another, a process known as transdifferentiation (3,4). Furthermore, although TH17 cell instability/plasticity has been associated with pathogenicity (1,2,5), it is unknown whether this could present a therapeutic opportunity, whereby formerly pathogenic TH17 cells could adopt an anti-inflammatory fate. Here we used two new fate-mapping mouse models to track TH17 cells during immune responses to show that [CD4.sup.+] T cells that formerly expressed IL-17A go on to acquire an antiinflammatory phenotype. The transdifferentiation of TH17 into regulatory T cells was illustrated by a change in their signature transcriptional profile and the acquisition of potent regulatory capacity. Comparisons of the transcriptional profiles of pre- and post-conversion TH17 cells also revealed a role for canonical TGF-β signalling and consequently for the aryl hydrocarbon receptor (AhR) in conversion. Thus, TH17 cells transdifferentiate into regulatory cells, and contribute to the resolution of inflammation. Our data suggest that TH17 cell instability and plasticity is a therapeutic opportunity for inflammatory diseases., TH17 cells are characterized by secretion of IL-17A, expression of chemokine receptor CCR6 and transcriptional factor RORγt (6). Their pathogenicity is limited by [Foxp3.sup.+] [T.sub.Reg] and T regulatory type 1 [...]
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- 2015
9. miR-33a/b contribute to the regulation of fatty acid metabolism and insulin signaling
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Dávalos, Alberto, Goedeke, Leigh, Smibert, Peter, Ramírez, Cristina M., Warrier, Nikhil P., Andreo, Ursula, Cirera-Salinas, Daniel, Rayner, Katey, Suresh, Uthra, Pastor-Pareja, José Carlos, Esplugues, Enric, Fisher, Edward A., Penalva, Luiz O. F., Moore, Kathryn J., Suárez, Yajaira, Lai, Eric C., and Fernández-Hernando, Carlos
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- 2011
10. Control of [T.sub.H]17 cells occurs in the small intestine
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Esplugues, Enric, Huber, Samuel, Gagliani, Nicola, Hauser, Anja E., Town, Terrence, Wan, Yisong Y., O'Connor, Jr., William, Rongvaux, Anthony, Van Rooijen, Nico, Haberman, Ann M., Iwakura, Yoichiro, Kuchroo, Vijay K., Kolls, Jay K., Bluestone, Jeffrey A., Herold, Kevan C., and Flavell, Richard A.
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T cells -- Health aspects -- Genetic aspects ,Intestine, Small -- Genetic aspects -- Health aspects ,Inflammatory bowel diseases -- Causes of -- Genetic aspects ,Environmental issues ,Science and technology ,Zoology and wildlife conservation - Abstract
Interleukin (IL)-17-producing T helper cells ([T.sub.H]17) are a recently identified [CD4.sup.+] T cell subset distinct from T helper type 1 ([T.sub.H]1) and T helper type 2 ([T.sub.H]2) cells (1). [T.sub.H]17 cells can drive antigenspecific autoimmune diseases and are considered the main population of pathogenic T cells driving experimental autoimmune encephalomyelitis (EAE) (2), the mouse model for multiple sclerosis. The factors that are needed for the generation of [T.sub.H]17 cells have been well characterized (3-6). However, where and how the immune system controls [T.sub.H]17 cells in vivo remains unclear. Here, by using a model of tolerance induced by CD3-specific antibody, a model of sepsis and influenza A viral infection (H1N1), we show that pro-inflammatory [T.sub.H]17 cells can be redirected to and controlled in the small intestine. [T.sub.H]17-specific IL-17A secretion induced expression of the chemokine CCL20 in the small intestine, facilitating the migration of these cells specifically to the small intestine via the CCR6/CCL20 axis. Moreover, we found that [T.sub.H]17 cells are controlled by two different mechanisms in the small intestine: first, they are eliminated via the intestinal lumen; second, pro-inflammatory [T.sub.H]17 cells simultaneously acquire a regulatory phenotype with invitro and invivoimmune-suppressive properties (r[T.sub.H]17). These results identify mechanisms limiting [T.sub.H]17 cell pathogenicity and implicate the gastrointestinal tract as a site for control of [T.sub.H]17 cells., [T.sub.H]17 cells have been associated with the pathogenesis of several chronic inflammatory disorders, including rheumatoid arthritis and multiple sclerosis (2,7). To study the cellular and molecular mechanisms that control pathogenicity [...]
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- 2011
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11. GERMINAL CENTERS: Dynamic signaling by T follicular helper cells during germinal center B cell selection
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Shulman, Ziv, Gitlin, Alexander D., Weinstein, Jason S., Lainez, Begoña, Esplugues, Enric, Flavell, Richard A., Craft, Joseph E., and Nussenzweig, Michel C.
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- 2014
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12. Th17 Cells Express Interleukin-10 Receptor and Are Controlled by Foxp3− and Foxp3+ Regulatory CD4+ T Cells in an Interleukin-10-Dependent Manner
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Huber, Samuel, Gagliani, Nicola, Esplugues, Enric, OʼConnor, William, Jr., Huber, Francis J., Chaudhry, Ashutosh, Kamanaka, Masahito, Kobayashi, Yasushi, Booth, Carmen J., Rudensky, Alexander Y., Roncarolo, Maria Grazia, Battaglia, Manuela, and Flavell, Richard A.
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- 2011
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13. CD69 limits early inflammatory diseases associated with immune response to Listeria monocytogenes infection
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Vega-Ramos, Javier, Alari-Pahissa, Elisenda, del Valle, Juana, Carrasco-Marín, Eugenio, Esplugues, Enric, Borràs, Miquel, Martínez-A, Carlos, and Lauzurica, Pilar
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- 2010
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14. Identification and characterization of a novel spliced variant that encodes human soluble tumor necrosis factor receptor 2
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Lainez, Begoña, Fernandez-Real, José Manuel, Romero, Xavier, Esplugues, Enric, Cañete, Juan D., Ricart, Wifredo, and Engel, Pablo
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- 2004
15. CD69 downregulates autoimmune reactivity through active transforming growth factor-β production in collagen-induced arthritis
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Sancho, David, Gómez, Manuel, Viedma, Fernando, Esplugues, Enric, Gordón-Alonso, Mónica, García-López, María Angeles, de la Fuente, Hortensia, Martínez-A, Carlos, Lauzurica, Pilar, and Sánchez-Madrid, Francisco
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- 2003
16. Intestinal type 1 regulatory T cells migrate to periphery to suppress diabetogenic T cells and prevent diabetes development.
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Hua Yu, Gagliani, Nicola, Ishigame, Harumichi, Huber, Samuel, Shu Zhu, Esplugues, Enric, Herold, Kevan C., Li Wen, and Flavell, Richard A.
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AUTOIMMUNE diseases ,PEOPLE with diabetes ,T cells ,CHEMOKINE receptors ,TRANSGENIC animals - Abstract
Growing insight into the pathogenesis of autoimmune diseases and numerous studies in preclinical models highlights the potential of regulatory T cells to restore tolerance. By using non-obese diabetic (NOD) BDC2.5 TCR-transgenic (Tg), and IL-10 and Foxp3 doublereporter mice, we demonstrate that alteration of gut microbiota during cohousing experiments or treatment with anti-CD3 mAb significantly increase intestinal IL-10-producing type 1 regulatory T (Tr1) cells and decrease diabetes incidence. These intestinal antigenspecific Tr1 cells have the ability to migrate to the periphery via a variety of chemokine receptors such as CCR4, CCR5, and CCR7 and to suppress proliferation of Th1 cells in the pancreas. The ability of Tr1 cells to cure diabetes in NOD mice required IL-10 signaling, as Tr1 cells could not suppress CD4
+ T cells with a dominant-negative IL-10R. Taken together, our data show a key role of intestinal Tr1 cells in the control of effector T cells and development of diabetes. Therefore, modulating gut-associated lymphoid tissue to boost Tr1 cells may be important in type 1 diabetes management. [ABSTRACT FROM AUTHOR]- Published
- 2017
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17. Gatekeeper role of brain antigen-presenting CD11c+ cells in neuroinflammation.
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Paterka, Magdalena, Siffrin, Volker, Voss, Jan O, Werr, Johannes, Hoppmann, Nicola, Gollan, René, Belikan, Patrick, Bruttger, Julia, Birkenstock, Jérôme, Jung, Steffen, Esplugues, Enric, Yogev, Nir, Flavell, Richard A, Bopp, Tobias, and Zipp, Frauke
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ANTIGEN presenting cells ,CD11 antigen ,MULTIPLE sclerosis ,AUTOIMMUNE diseases ,CELL communication ,CHEMOKINES - Abstract
Multiple sclerosis is the most frequent chronic inflammatory disease of the CNS. The entry and survival of pathogenic T cells in the CNS are crucial for the initiation and persistence of autoimmune neuroinflammation. In this respect, contradictory evidence exists on the role of the most potent type of antigenpresenting cells, dendritic cells. Applying intravital two-photon microscopy, we demonstrate the gatekeeper function of CNS professional antigen-presenting CD11c
+ cells, which preferentially interact with Th17 cells. IL-17 expression correlates with expression of GM-CSF by T cells and with accumulation of CNS CD11c+ cells. These CD11c+ cells are organized in perivascular clusters, targeted by T cells, and strongly express the inflammatory chemokines Ccl5, Cxcl9, and Cxcl10. Our findings demonstrate a fundamental role of CNS CD11c+ cells in the attraction of pathogenic T cells into and their survival within the CNS. Depletion of CD11c+ cells markedly reduced disease severity due to impaired enrichment of pathogenic T cells within the CNS. [ABSTRACT FROM AUTHOR]- Published
- 2016
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18. A Critical Role of IL-21-Induced BATF in Sustaining CD8-T-Cell-Mediated Chronic Viral Control.
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Xin, Gang, Schauder, David M., Lainez, Begoña, Weinstein, Jason S., Dai, Zhengxi, Chen, Yuhong, Esplugues, Enric, Wen, Renren, Wang, Demin, Parish, Ian A., Zajac, Allan J., Craft, Joe, and Cui, Weiguo
- Abstract
Summary Control of chronic viral infections by CD8 T cells is critically dependent on CD4 help. In particular, helper-derived IL-21 plays a key role in sustaining the CD8 T cell response; however, the molecular pathways by which IL-21 sustains CD8 T cell immunity remain unclear. We demonstrate that IL-21 causes a phenotypic switch of transcription factor expression in CD8 T cells during chronic viral infection characterized by sustained BATF expression. Importantly, BATF expression during chronic infection is both required for optimal CD8 T cell persistence and anti-viral effector function and sufficient to rescue “unhelped” CD8 T cells. Mechanistically, BATF sustains the response by cooperating with IRF4, an antigen-induced transcription factor that is also critically required for CD8 T cell maintenance, to preserve Blimp-1 expression and thereby sustain CD8 T cell effector function. Collectively, these data suggest that CD4 T cells “help” the CD8 response during chronic infection via IL-21-induced BATF expression. [ABSTRACT FROM AUTHOR]
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- 2015
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19. Oct-1 Regulates IL-17 Expression by Directing Interchromosomal Associations in Conjunction with CTCF in T Cells.
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Kim, Lark?Kyun, Esplugues, Enric, Zorca, Cornelia?E., Parisi, Fabio, Kluger, Yuval, Kim, Tae Hoon, Galjart, Niels?J., and Flavell, Richard?A.
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INTERLEUKIN-17 , *T cells , *PLANT chromosomes , *GENE expression in plants , *INTERLEUKIN-4 , *CELL differentiation - Abstract
Summary: Interchromosomal associations can regulate gene expression, but little is known about the molecular basis of such associations. In response to antigen stimulation, naive T cells can differentiate into Th1, Th2, and Th17 cells expressing IFN-γ, IL-4, and IL-17, respectively. We previously reported that in naive T cells, the IFN-γ locus is associated with the Th2 cytokine locus. Here we show that the Th2 locus additionally associates with the IL-17 locus. This association requires a DNase I hypersensitive region (RHS6) at the Th2 locus. RHS6 and the IL-17 promoter both bear Oct-1 binding sites. Deletion of either of these sites or Oct-1 gene impairs the association. Oct-1 and CTCF bind their cognate sites cooperatively, and CTCF deficiency similarly impairs the association. Finally, defects in the association lead to enhanced IL-17 induction. Collectively, our data indicate Th17 lineage differentiation is restrained by the Th2 locus via interchromosomal associations organized by Oct-1 and CTCF. [ABSTRACT FROM AUTHOR]
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- 2014
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20. The Role of TH17-Associated Cytokines in Health and Disease.
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O'Connor Jr., William, Esplugues, Enric, and Huber, Samuel
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CYTOKINE genetics , *DISEASES , *MANAGEMENT , *HIV infections , *CELL proliferation ,HEALTH management - Published
- 2014
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21. Effector CD4+ T Cell Expression Signatures and Immune-Mediated Disease Associated Genes.
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Wei Zhang, Ferguson, John, Sok Meng Ng, Ken Hui, Gerald Goh, Aiping Lin, Esplugues, Enric, Flavell, Richard A., Abraham, Clara, Hongyu Zhao, and Judy H. Cho
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T cells ,GENES ,GENETICS ,HEREDITY ,GENOMES - Abstract
Genome-wide association studies (GWAS) in immune-mediated diseases have identified over 150 associated genomic loci. Many of these loci play a role in T cell responses, and regulation of T cell differentiation plays a critical role in immunemediated diseases; however, the relationship between implicated disease loci and T cell differentiation is incompletely understood. To further address this relationship, we examined differential gene expression in naïve human CD4+ T cells, as well as in in vitro differentiated Th1, memory Th17-negative and Th17-enriched CD4+ T cells subsets using microarray and RNASeq. We observed a marked enrichment for increased expression in memory CD4+ compared to naïve CD4+ T cells of genes contained among immune-mediated disease loci. Within memory T cells, expression of disease-associated genes was typically increased in Th17-enriched compared to Th17-negative cells. Utilizing RNASeq and promoter methylation studies, we identified a differential regulation pattern for genes solely expressed in Th17 cells (IL17A and CCL20) compared to genes expressed in both Th17 and Th1 cells (IL23R and IL12RB2), where high levels of promoter methylation are correlated to near zero RNASeq levels for IL17A and CCL20. These findings have implications for human Th17 celI plasticity and for the regulation of Th17-Th1 expression signatures. Importantly, utilizing RNASeq we found an abundant isoform of IL23R terminating before the transmembrane domain that was enriched in Th17 cells. In addition to molecular resolution, we find that RNASeq provides significantly improved power to define differential gene expression and identify alternative gene variants relative to microarray analysis. The comprehensive integration of differential gene expression between cell subsets with disease-association signals, and functional pathways provides insight into disease pathogenesis. [ABSTRACT FROM AUTHOR]
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- 2012
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22. Mir-33 regulates cell proliferation and cell cycle progression.
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Cirera-Salinas, Daniel, Pauta, Montse, Allen, Ryan M., Salerno, Alessandro G., Ramírez, Cristina M., Chamorro-Jorganes, Aránzazu, Wanschel, Amarylis C., Lasunción, Miguel A., Morales-Ruiz, Manuel, Suárez, Yajaira, Baldán, Ángel, Esplugues, Enric, and Fernández-Hernando, Carlos
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- 2012
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23. Development of Autoimmune Diabetes in the Absence of Detectable IL-17A in a CD8-Driven Virally Induced Model.
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Van Belle, Tom L., Esplugues, Enric, Liao, Jeanette, Juntti, Therese, Flavell, Richard A., and von Herrath, Matthias G.
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DIAGNOSIS of diabetes , *AUTOIMMUNE disease diagnosis , *GREEN fluorescent protein , *LABORATORY mice , *KLEBSIELLA pneumoniae , *LYMPHOCYTIC choriomeningitis virus , *THERAPEUTICS - Abstract
Recent studies have shown that IL-17 can contribute beneficially to pathogen defense but also that excessive IL-17 levels are associated with chronic inflammation and autoimmune disorders. To date, the role of IL-17 in viral infections and type 1 diabetes is ambiguous. In this study, we used IL-17A enhanced green fluorescent protein bicistronic reporter mouse strains to analyze in situ production of IL-17A. Upon Klebsiella pneumoniae bacterial infection, CD4+ and γδ T cells produce IL-17A. In contrast, CD4+ or CD8+ T cells do not produce IL-17A in response to acute or protracted viral infection with lymphocytic choriomeningitis virus or during autoimmune diabetes development in the CD8-driven lymphocytic choriomeningitis virus-induced model of type 1 diabetes. We conclude that viral elimination and type 1 diabetes can occur in the absence of detectable IL-17A production, suggesting IL-17A is not essential in these settings. [ABSTRACT FROM AUTHOR]
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- 2011
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24. Control of TH17 cells occurs in the small intestine.
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Esplugues, Enric, Huber, Samuel, Gagliani, Nicola, Hauser, Anja E., Town, Terrence, Wan, Yisong Y., O'Connor, William, Rongvaux, Anthony, Van Rooijen, Nico, Haberman, Ann M., Iwakura, Yoichiro, Kuchroo, Vijay K., Kolls, Jay K., Bluestone, Jeffrey A., Herold, Kevan C., and Flavell, Richard A.
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T cell differentiation , *INTERLEUKINS , *CD4 antigen , *ENCEPHALOMYELITIS , *ANIMAL models of multiple sclerosis , *CHEMOKINES - Abstract
Interleukin (IL)-17-producing T helper cells (TH17) are a recently identified CD4+ T cell subset distinct from T helper type 1 (TH1) and T helper type 2 (TH2) cells. TH17 cells can drive antigen-specific autoimmune diseases and are considered the main population of pathogenic T cells driving experimental autoimmune encephalomyelitis (EAE), the mouse model for multiple sclerosis. The factors that are needed for the generation of TH17 cells have been well characterized. However, where and how the immune system controls TH17 cells in vivo remains unclear. Here, by using a model of tolerance induced by CD3-specific antibody, a model of sepsis and influenza A viral infection (H1N1), we show that pro-inflammatory TH17 cells can be redirected to and controlled in the small intestine. TH17-specific IL-17A secretion induced expression of the chemokine CCL20 in the small intestine, facilitating the migration of these cells specifically to the small intestine via the CCR6/CCL20 axis. Moreover, we found that TH17 cells are controlled by two different mechanisms in the small intestine: first, they are eliminated via the intestinal lumen; second, pro-inflammatory TH17 cells simultaneously acquire a regulatory phenotype with in vitro and in vivo immune-suppressive properties (rTH17). These results identify mechanisms limiting TH17 cell pathogenicity and implicate the gastrointestinal tract as a site for control of TH17 cells. [ABSTRACT FROM AUTHOR]
- Published
- 2011
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25. The role of TH17-associated cytokines in health and disease.
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O'Connor Jr, William, Esplugues, Enric, Huber, Samuel, and O'Connor, William Jr
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- 2014
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26. Interleukin-17A is not Produced by T Cells During Anti-viral Immunity and Autoimmunity in a Model of Virus-induced Autoimmune Diabetes
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Van Belle, Tom, Esplugues, Enric, Liao, Jeanette, Juntti, Therese, Flavell, Richard, and von Herrath, Matthias
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- 2010
- Full Text
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27. Th17 Cells Express Interleukin-10 Receptor and Are Controlled by Foxp3− and Foxp3+ Regulatory CD4+ T Cells in an Interleukin-10-Dependent Manner
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Huber, Samuel, Gagliani, Nicola, Esplugues, Enric, O'Connor, William, Huber, Francis J., Chaudhry, Ashutosh, Kamanaka, Masahito, Kobayashi, Yasushi, Booth, Carmen J., Rudensky, Alexander Y., Roncarolo, Maria Grazia, Battaglia, Manuela, and Flavell, Richard A.
- Subjects
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GLYCOPROTEINS , *T cells , *INTERLEUKINS , *AUTOIMMUNE diseases , *ENTERITIS , *LABORATORY mice , *THERAPEUTICS - Abstract
Summary: T helper 17 (Th17) cells are important for host defense against extracellular microorganisms. However, they are also implicated in autoimmune and chronic inflammatory diseases, and as such need to be tightly regulated. The mechanisms that directly control committed pathogenic Th17 cells in vivo remain unclear. We showed here that IL-17A-producing CD4+ T cells expressed interleukin-10 receptor α (IL-10Rα) in vivo. Importantly, T cell-specific blockade of IL-10 signaling led to a selective increase of IL-17A+IFN-γ− (Th17) and IL-17A+IFN-γ+ (Th17+Th1) CD4+ T cells during intestinal inflammation in the small intestine. CD4+Foxp3− IL-10-producing (Tr1) cells and CD4+Foxp3+ regulatory (Treg) cells were able to control Th17 and Th17+Th1 cells in an IL-10-dependent manner in vivo. Lastly, IL-10 treatment of mice with established colitis decreased Th17 and Th17+Th1 cell frequencies via direct signaling in T cells. Thus, IL-10 signaling directly suppresses Th17 and Th17+Th1 cells. [Copyright &y& Elsevier]
- Published
- 2011
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28. IL-10 Receptor Signaling Is Essential for TR1 Cell Function In Vivo.
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Brockmann, Leonie, Gagliani, Nicola, Steglich, Babett, Giannou, Anastasios D., Kempski, Jan, Pelczar, Penelope, Geffken, Maria, Mfarrej, Bechara, Huber, Francis, Herkel, Johannes, Wan, Yisong Y., Esplugues, Enric, Battaglia, Manuela, Krebs, Christian F., Flavell, Richard A., and Huber, Samuel
- Subjects
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CELL physiology , *HOMEOSTASIS , *CYTOKINES , *LABORATORY mice , *T cells , *THERAPEUTICS - Abstract
IL-10 is essential to maintain intestinal homeostasis. CD4+ T regulatory type 1 (TR1) cells produce large amounts of this cytokine and are therefore currently being examined in clinical trials as T cell therapy in patients with inflammatory bowel disease. However, factors and molecular signals sustaining TR1 cell regulatory activity still need to be identified to optimize the efficiency and ensure the safety of these trials. We investigated the role of IL-10 signaling in mature TR1 cells in vivo. Double IL-10eGFP Foxp3mRFP reporter mice and transgenic mice with impairment in IL-10 receptor signaling were used to test the activity of TR1 cells in a murine inflammatory bowel disease model, a model that resembles the trials performed in humans. The molecular signaling was elucidated in vitro. Finally, we used human TR1 cells, currently employed for cell therapy, to confirm our results. We found that murine TR1 cells expressed functional IL-10Ra. TR1 cells with impaired IL-10 receptor signaling lost their regulatory activity in vivo. TR1 cells required IL-10 receptor signaling to activate p38 MAPK, thereby sustaining IL-10 production, which ultimately mediated their suppressive activity. Finally, we confirmed these data using human TR1 cells. In conclusion, TR1 cell regulatory activity is dependent on IL-10 receptor signaling. These data suggest that to optimize TR1 cell-based therapy, IL-10 receptor expression has to be taken into consideration. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
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29. Dynamic signaling by T follicular helper cells during germinal center B cell selection.
- Author
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Shulman, Ziv, Gitlin, Alexander D., Weinstein, Jason S., Lainez, Begoña, Esplugues, Enric, Flavell, Richard A., Craft, Joseph E., and Nussenzweig, Michel C.
- Subjects
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CELL communication , *T helper cells , *B cells , *GERMINAL centers , *INTRACELLULAR calcium , *INTERLEUKIN-4 , *INTERLEUKIN-21 , *CELL migration inhibition - Abstract
T follicular helper (TFH) cells select high-affinity, antibody-producing B cells for clonal expansion in germinal centers (GCs), but the nature of their interaction is not well defined. Using intravital imaging, we found that selection is mediated by large but transient contacts between TFH and GC B cells presenting the highest levels of cognate peptide bound to major histocompatibility complex II. These interactions elicited transient and sustained increases in TFH intracellular free calcium (Ca2+) that were associated with TFH cell coexpression of the cytokines interleukin-4 and -21. However, increased intracellular Ca2+ did not arrest TFH cell migration. Instead, TFH cells remained motile and continually scanned the surface of many GC B cells, forming short-lived contacts that induced selection through further repeated transient elevations in intracellular Ca2+. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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30. Enhanced Anti-Serpin Antibody Activity Inhibits Autoimmune Inflammation in Type 1 Diabetes.
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Czyzyk, Jan, Henegariu, Octavian, Preston-Hurlburt, Paula, Baldzizhar, Raman, Fedorchuk, Christine, Esplugues, Enric, Bottomly, Kim, Gorus, Frans K., Herold, Kevan, and Flavell, Richard A.
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- *
AUTOIMMUNE diseases , *INFLAMMATION , *DIABETES , *SERPINS , *PROTEASE inhibitors , *HOMEOSTASIS , *HUMORAL immunity - Abstract
Intracellular (clade B) OVA-serpin protease inhibitors play an important role in tissue homeostasis by protecting cells from death in response to hypo-osmotic stress, heat shock, and other stimuli. It is not known whether these serpins influence immunological tolerance and the risk for autoimmune diseases. We found that a fraction of young autoimmune diabetes-prone NOD mice had elevated levels of autoantibodies against a member of clade B family known as serpinB13. High levels of anti-serpinB13 Abs were accompanied by low levels of anti-insulin autoantibodies, reduced numbers of islet-associated T cells, and delayed onset of diabetes. Exposure to anti-serpinB13 mAb alone also decreased islet inflammation, and coadministration of this reagent and a suboptimal dose of anti-CD3 mAb accelerated recovery from diabetes. In a fashion similar to that discovered in the NOD model, a deficiency in humoral activity against serpinB13 was associated with early onset of human type 1 diabetes. These findings suggest that, in addition to limiting exposure to proteases within the cell, clade B serpins help to maintain homeostasis by inducing protective humoral immunity. [ABSTRACT FROM AUTHOR]
- Published
- 2012
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31. SARS-CoV-2-encoded small RNAs are able to repress the host expression of SERINC5 to facilitate viral replication.
- Author
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Meseguer S, Rubio MP, Lainez B, Pérez-Benavente B, Pérez-Moraga R, Romera-Giner S, García-García F, Martinez-Macias O, Cremades A, Iborra FJ, Candelas-Rivera O, Almazan F, and Esplugues E
- Abstract
Serine incorporator protein 5 (SERINC5) is a key innate immunity factor that operates in the cell to restrict the infectivity of certain viruses. Different viruses have developed strategies to antagonize SERINC5 function but, how SERINC5 is controlled during viral infection is poorly understood. Here, we report that SERINC5 levels are reduced in COVID-19 patients during the infection by SARS-CoV-2 and, since no viral protein capable of repressing the expression of SERINC5 has been identified, we hypothesized that SARS-CoV-2 non-coding small viral RNAs (svRNAs) could be responsible for this repression. Two newly identified svRNAs with predicted binding sites in the 3'-untranslated region (3'-UTR) of the SERINC5 gene were characterized and we found that the expression of both svRNAs during the infection was not dependent on the miRNA pathway proteins Dicer and Argonaute-2. By using svRNAs mimic oligonucleotides, we demonstrated that both viral svRNAs can bind the 3'UTR of SERINC5 mRNA, reducing SERINC5 expression in vitro . Moreover, we found that an anti-svRNA treatment to Vero E6 cells before SARS-CoV-2 infection recovered the levels of SERINC5 and reduced the levels of N and S viral proteins. Finally, we showed that SERINC5 positively controls the levels of Mitochondrial Antiviral Signalling (MAVS) protein in Vero E6. These results highlight the therapeutic potential of targeting svRNAs based on their action on key proteins of the innate immune response during SARS-CoV-2 viral infection., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Meseguer, Rubio, Lainez, Pérez-Benavente, Pérez-Moraga, Romera-Giner, García-García, Martinez-Macias, Cremades, Iborra, Candelas-Rivera, Almazan and Esplugues.)
- Published
- 2023
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32. Effector T H 17 Cells Give Rise to Long-Lived T RM Cells that Are Essential for an Immediate Response against Bacterial Infection.
- Author
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Amezcua Vesely MC, Pallis P, Bielecki P, Low JS, Zhao J, Harman CCD, Kroehling L, Jackson R, Bailis W, Licona-Limón P, Xu H, Iijima N, Pillai PS, Kaplan DH, Weaver CT, Kluger Y, Kowalczyk MS, Iwasaki A, Pereira JP, Esplugues E, Gagliani N, and Flavell RA
- Subjects
- Animals, CD4-Positive T-Lymphocytes cytology, CD4-Positive T-Lymphocytes immunology, CD4-Positive T-Lymphocytes metabolism, Diphtheria Toxin pharmacology, Disease Models, Animal, Female, Immunologic Memory, Interleukin-17 genetics, Interleukin-17 metabolism, Klebsiella Infections pathology, Klebsiella pneumoniae immunology, Klebsiella pneumoniae pathogenicity, Lung drug effects, Lung metabolism, Lung microbiology, Mice, Mice, Inbred C57BL, Th17 Cells cytology, Th17 Cells metabolism, Klebsiella Infections immunology, Th17 Cells immunology
- Abstract
Adaptive immunity provides life-long protection by generating central and effector memory T cells and the most recently described tissue resident memory T (T
RM ) cells. However, the cellular origin of CD4 TRM cells and their contribution to host defense remain elusive. Using IL-17A tracking-fate mouse models, we found that a significant fraction of lung CD4 TRM cells derive from IL-17A-producing effector (TH 17) cells following immunization with heat-killed Klebsiella pneumonia (Kp). These exTH 17 TRM cells are maintained in the lung by IL-7, produced by lymphatic endothelial cells. During a memory response, neither antibodies, γδ T cells, nor circulatory T cells are sufficient for the rapid host defense required to eliminate Kp. Conversely, using parabiosis and depletion studies, we demonstrated that exTH 17 TRM cells play an important role in bacterial clearance. Thus, we delineate the origin and function of airway CD4 TRM cells during bacterial infection, offering novel strategies for targeted vaccine design., (Copyright © 2019 Elsevier Inc. All rights reserved.)- Published
- 2019
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33. IL-10 Receptor Signaling Is Essential for TR1 Cell Function In Vivo.
- Author
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Brockmann L, Gagliani N, Steglich B, Giannou AD, Kempski J, Pelczar P, Geffken M, Mfarrej B, Huber F, Herkel J, Wan YY, Esplugues E, Battaglia M, Krebs CF, Flavell RA, and Huber S
- Subjects
- Animals, Interleukin-10 physiology, Mice, Mice, Inbred C57BL, Phosphorylation, STAT3 Transcription Factor metabolism, Th17 Cells immunology, p38 Mitogen-Activated Protein Kinases metabolism, Receptors, Interleukin-10 physiology, Signal Transduction physiology, T-Lymphocytes, Regulatory immunology
- Abstract
IL-10 is essential to maintain intestinal homeostasis. CD4
+ T regulatory type 1 (TR 1) cells produce large amounts of this cytokine and are therefore currently being examined in clinical trials as T cell therapy in patients with inflammatory bowel disease. However, factors and molecular signals sustaining TR 1 cell regulatory activity still need to be identified to optimize the efficiency and ensure the safety of these trials. We investigated the role of IL-10 signaling in mature TR 1 cells in vivo. Double IL-10eGFP Foxp3mRFP reporter mice and transgenic mice with impairment in IL-10 receptor signaling were used to test the activity of TR 1 cells in a murine inflammatory bowel disease model, a model that resembles the trials performed in humans. The molecular signaling was elucidated in vitro. Finally, we used human TR 1 cells, currently employed for cell therapy, to confirm our results. We found that murine TR 1 cells expressed functional IL-10Rα. TR 1 cells with impaired IL-10 receptor signaling lost their regulatory activity in vivo. TR 1 cells required IL-10 receptor signaling to activate p38 MAPK, thereby sustaining IL-10 production, which ultimately mediated their suppressive activity. Finally, we confirmed these data using human TR 1 cells. In conclusion, TR 1 cell regulatory activity is dependent on IL-10 receptor signaling. These data suggest that to optimize TR 1 cell-based therapy, IL-10 receptor expression has to be taken into consideration., Competing Interests: The authors have declared that there is no conflict of interest., (Copyright © 2017 by The American Association of Immunologists, Inc.)- Published
- 2017
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34. Gatekeeper role of brain antigen-presenting CD11c+ cells in neuroinflammation.
- Author
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Paterka M, Siffrin V, Voss JO, Werr J, Hoppmann N, Gollan R, Belikan P, Bruttger J, Birkenstock J, Jung S, Esplugues E, Yogev N, Flavell RA, Bopp T, and Zipp F
- Subjects
- Animals, Antigen-Presenting Cells chemistry, Brain immunology, Cell Movement, Dendritic Cells chemistry, Encephalomyelitis, Autoimmune, Experimental immunology, Granulocyte-Macrophage Colony-Stimulating Factor metabolism, Interleukin-17 metabolism, Mice, Inbred C57BL, T-Lymphocytes physiology, Th17 Cells physiology, Antigen-Presenting Cells physiology, Brain pathology, CD11c Antigen analysis, Dendritic Cells physiology, Encephalomyelitis, Autoimmune, Experimental pathology, T-Lymphocytes immunology
- Abstract
Multiple sclerosis is the most frequent chronic inflammatory disease of the CNS. The entry and survival of pathogenic T cells in the CNS are crucial for the initiation and persistence of autoimmune neuroinflammation. In this respect, contradictory evidence exists on the role of the most potent type of antigen-presenting cells, dendritic cells. Applying intravital two-photon microscopy, we demonstrate the gatekeeper function of CNS professional antigen-presenting CD11c(+) cells, which preferentially interact with Th17 cells. IL-17 expression correlates with expression of GM-CSF by T cells and with accumulation of CNS CD11c(+) cells. These CD11c(+) cells are organized in perivascular clusters, targeted by T cells, and strongly express the inflammatory chemokines Ccl5, Cxcl9, and Cxcl10. Our findings demonstrate a fundamental role of CNS CD11c(+) cells in the attraction of pathogenic T cells into and their survival within the CNS. Depletion of CD11c(+) cells markedly reduced disease severity due to impaired enrichment of pathogenic T cells within the CNS., (© 2015 The Authors.)
- Published
- 2016
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35. Bcl6 expression specifies the T follicular helper cell program in vivo.
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Liu X, Yan X, Zhong B, Nurieva RI, Wang A, Wang X, Martin-Orozco N, Wang Y, Chang SH, Esplugues E, Flavell RA, Tian Q, and Dong C
- Subjects
- Animals, Antibody Formation immunology, Cell Differentiation immunology, Cluster Analysis, DNA-Binding Proteins immunology, Gene Expression, Gene Expression Profiling, Gene Order, Gene Targeting, Genes, Reporter, Germinal Center immunology, Immunologic Memory, Mice, Mice, Transgenic, Proto-Oncogene Proteins c-bcl-6, Receptors, CXCR5 genetics, Receptors, CXCR5 metabolism, T-Lymphocyte Subsets cytology, T-Lymphocyte Subsets immunology, T-Lymphocyte Subsets metabolism, T-Lymphocytes, Helper-Inducer cytology, T-Lymphocytes, Helper-Inducer metabolism, Transcription, Genetic, DNA-Binding Proteins genetics, T-Lymphocytes, Helper-Inducer immunology
- Abstract
T follicular helper cells (Tfh cells) play a pivotal role in germinal center reactions, which require B cell lymphoma 6 (Bcl6) transcription factor. To analyze their relationships with other effector T cell lineages and their stability in vivo, we developed and analyzed a new Bcl6 reporter mouse alone or together with other lineage reporter systems. Assisted with genome-wide transcriptome analysis, we show substantial plasticity of T cell differentiation in the early phase of immune response. At this stage, CXCR5 appears to be expressed in a Bcl6-independent manner. Once Bcl6 is highly expressed, Tfh cells can persist in vivo and some of them develop into memory cells. Together, our results indicate Bcl6 as a bona fide marker for Tfh polarized program.
- Published
- 2012
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36. Effector CD4+ T cell expression signatures and immune-mediated disease associated genes.
- Author
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Zhang W, Ferguson J, Ng SM, Hui K, Goh G, Lin A, Esplugues E, Flavell RA, Abraham C, Zhao H, and Cho JH
- Subjects
- Chemokine CCL20 immunology, Female, Genome-Wide Association Study, Humans, Immune System Diseases pathology, Interleukin-17 immunology, Male, Receptors, Interleukin immunology, Receptors, Interleukin-12 immunology, Th1 Cells pathology, Th17 Cells pathology, Gene Expression Regulation immunology, Immune System Diseases immunology, Th1 Cells immunology, Th17 Cells immunology
- Abstract
Genome-wide association studies (GWAS) in immune-mediated diseases have identified over 150 associated genomic loci. Many of these loci play a role in T cell responses, and regulation of T cell differentiation plays a critical role in immune-mediated diseases; however, the relationship between implicated disease loci and T cell differentiation is incompletely understood. To further address this relationship, we examined differential gene expression in naïve human CD4+ T cells, as well as in in vitro differentiated Th1, memory Th17-negative and Th17-enriched CD4+ T cells subsets using microarray and RNASeq. We observed a marked enrichment for increased expression in memory CD4+ compared to naïve CD4+ T cells of genes contained among immune-mediated disease loci. Within memory T cells, expression of disease-associated genes was typically increased in Th17-enriched compared to Th17-negative cells. Utilizing RNASeq and promoter methylation studies, we identified a differential regulation pattern for genes solely expressed in Th17 cells (IL17A and CCL20) compared to genes expressed in both Th17 and Th1 cells (IL23R and IL12RB2), where high levels of promoter methylation are correlated to near zero RNASeq levels for IL17A and CCL20. These findings have implications for human Th17 celI plasticity and for the regulation of Th17-Th1 expression signatures. Importantly, utilizing RNASeq we found an abundant isoform of IL23R terminating before the transmembrane domain that was enriched in Th17 cells. In addition to molecular resolution, we find that RNASeq provides significantly improved power to define differential gene expression and identify alternative gene variants relative to microarray analysis. The comprehensive integration of differential gene expression between cell subsets with disease-association signals, and functional pathways provides insight into disease pathogenesis.
- Published
- 2012
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37. CD69 targeting differentially affects the course of collagen-induced arthritis.
- Author
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Sancho D, Gómez M, Martinez Del Hoyo G, Lamana A, Esplugues E, Lauzurica P, Martinez-A C, and Sánchez-Madrid F
- Subjects
- Adoptive Transfer methods, Animals, Antibodies, Monoclonal therapeutic use, Antigens, CD genetics, Antigens, Differentiation, T-Lymphocyte genetics, Arthritis, Experimental drug therapy, Arthritis, Experimental genetics, Cell Proliferation drug effects, Collagen Type II toxicity, Immunoglobulin G therapeutic use, Inflammation chemically induced, Inflammation drug therapy, Inflammation genetics, Inflammation immunology, Lectins, C-Type, Lymphocyte Depletion methods, Mice, Mice, Inbred BALB C, Mice, Inbred DBA, Mice, Knockout, Antibodies, Monoclonal immunology, Antigens, CD immunology, Antigens, Differentiation, T-Lymphocyte immunology, Arthritis, Experimental immunology, Immunoglobulin G immunology, Interferon-gamma immunology, T-Lymphocytes immunology
- Abstract
CD69 expression is induced following activation of leukocytes at inflammatory sites and plays a negative regulatory role in the development of collagen-induced arthritis (CIA). To evaluate potential strategies of CD69 targeting in chronic inflammatory diseases, two different anti-CD69 mAbs were generated and their effects on CIA were studied. Administration of the IgG1 anti-CD69 mAb 2.2 to DBA/1 mice with CIA led to an exacerbation of the disease, correlated with down-modulation of CD69 from the cell surface, and reproduced the phenotype of the CD69(-/-) mouse in wild-type animals. In contrast, treatment with the IgG2a anti-CD69 mAb 2.3 was effective in ameliorating CIA when administered in the early or intermediate phases of the disease, causing a decreased production of proinflammatory cytokines in inflammatory foci. Monoclonal antibody 2.3 induces partial depletion of CD69+ cells in vivo. Moreover, adoptive transfer of type-II collagen (CII)-sensitized cells treated with mAb 2.3 to deplete CD69+ cells did not result in arthritis. The attenuation of inflammation correlates with reduced lymphocyte proliferative response in response to CII and with a reduction in the frequency of CII-specific T cells producing IFN-gamma. We thus conclude that CD69 targeting by mAbs can either enhance or dampen the immune response.
- Published
- 2006
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- View/download PDF
38. The adaptor protein 3BP2 binds human CD244 and links this receptor to Vav signaling, ERK activation, and NK cell killing.
- Author
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Saborit-Villarroya I, Del Valle JM, Romero X, Esplugues E, Lauzurica P, Engel P, and Martín M
- Subjects
- Adaptor Proteins, Signal Transducing physiology, Animals, Antigens, CD physiology, Cell Line, Tumor, Coculture Techniques, Extracellular Signal-Regulated MAP Kinases physiology, Humans, Interferon-gamma metabolism, Ligands, Membrane Glycoproteins physiology, Mice, Phosphorylation, Receptors, Immunologic physiology, Signal Transduction physiology, Signaling Lymphocytic Activation Molecule Family, Yeasts metabolism, Adaptor Proteins, Signal Transducing metabolism, Antigens, CD metabolism, Cytotoxicity, Immunologic, Extracellular Signal-Regulated MAP Kinases metabolism, Killer Cells, Natural physiology, Membrane Glycoproteins metabolism, Receptors, Immunologic metabolism
- Abstract
Adaptor proteins, molecules that mediate intermolecular interactions, are crucial for cellular activation. The adaptor 3BP2 has been shown to positively regulate NK cell-mediated cytotoxicity. In this study we present evidence for a physical interaction between 3BP2 and the CD244 receptor. CD244, a member of the CD150 family, is a cell surface protein expressed on NK, CD8+ T, and myeloid cells. CD244 interacts via its Src homology 2 domain with the X-linked lymphoproliferative disease gene product signaling lymphocytic activation molecule-associated protein (SAP)/SH2 domain protein 1A. 3BP2 interacts with human but not murine CD244. CD244-3BP2 interaction was direct and regulated by phosphorylation, as shown by a three-hybrid analysis in yeast and NK cells. Tyr337 on CD244, part of a consensus motif for SAP/SH2 domain protein 1A binding, was critical for the 3BP2 interaction. Although mutation of Tyr337 to phenylalanine abrogated human 3BP2 binding, we still observed SAP association, indicating that this motif is not essential for SAP recruitment. CD244 ligation induced 3BP2 phosphorylation and Vav-1 recruitment. Overexpression of 3BP2 led to an increase in the magnitude and duration of ERK activation, after CD244 triggering. This enhancement was concomitant with an increase in cytotoxicity due to CD244 ligation. However, no differences in IFN-gamma secretion were found when normal and 3BP2-transfected cells were compared. These results indicate that CD244-3BP2 association regulates cytolytic function but not IFN-gamma release, reinforcing the hypothesis that, in humans, CD244-mediated cytotoxicity and IFN-gamma release involve distinct NK pathways.
- Published
- 2005
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39. Induction of tumor NK-cell immunity by anti-CD69 antibody therapy.
- Author
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Esplugues E, Vega-Ramos J, Cartoixà D, Vazquez BN, Salaet I, Engel P, and Lauzurica P
- Subjects
- Animals, Antibodies, Monoclonal administration & dosage, Cytotoxicity, Immunologic, Immunity drug effects, Interferon-gamma biosynthesis, Killer Cells, Natural drug effects, Lectins, C-Type, Lung Neoplasms drug therapy, Lung Neoplasms immunology, Lung Neoplasms secondary, Lymphocyte Activation drug effects, Lymphoma drug therapy, Lymphoma immunology, Male, Mice, Mice, Inbred C57BL, Neoplasms immunology, Prostatic Neoplasms drug therapy, Prostatic Neoplasms pathology, Antibodies, Monoclonal therapeutic use, Antigens, CD immunology, Antigens, Differentiation, T-Lymphocyte immunology, Immunotherapy methods, Killer Cells, Natural immunology, Neoplasms therapy
- Abstract
The leukocyte activation marker CD69 is a novel regulator of the immune response, modulating the production of cytokines including transforming growth factor-beta (TGF-beta). We have generated an antimurine CD69 monoclonal antibody (mAb), CD69.2.2, which down-regulates CD69 expression in vivo but does not deplete CD69-expressing cells. Therapeutic administration of CD69.2.2 to wild-type mice induces significant natural killer (NK) cell-dependent antitumor responses to major histocompatibility complex (MHC) class I low RMA-S lymphomas and to RM-1 prostatic carcinoma lung metastases. These in vivo antitumor responses are comparable to those seen in CD69(-/-) mice. Enhanced host NK cytotoxic activity correlates with a reduction in NK-cell TGF-beta production and is independent of tumor priming. In vitro studies demonstrate the novel ability of anti-CD69 mAbs to activate resting NK cells in an Fc receptor-independent manner, resulting in a substantial increase in both NK-cell cytolytic activity and interferon gamma (IFNgamma) production. Modulation of the innate immune system with monoclonal antibodies to host CD69 thus provides a novel means to antagonize tumor growth and metastasis.
- Published
- 2005
- Full Text
- View/download PDF
40. CD69 downregulates autoimmune reactivity through active transforming growth factor-beta production in collagen-induced arthritis.
- Author
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Sancho D, Gómez M, Viedma F, Esplugues E, Gordón-Alonso M, García-López MA, de la Fuente H, Martínez-A C, Lauzurica P, and Sánchez-Madrid F
- Subjects
- Animals, Antigens, CD genetics, Antigens, CD immunology, Antigens, Differentiation, T-Lymphocyte genetics, Antigens, Differentiation, T-Lymphocyte immunology, Arthritis, Experimental immunology, Cytokines genetics, Cytokines immunology, Cytokines metabolism, Foot pathology, Humans, Inflammation immunology, Joints cytology, Joints pathology, Lectins, C-Type, Mice, Mice, Inbred C57BL, Mice, Knockout, Spleen cytology, Spleen pathology, Synovial Fluid cytology, Synovial Fluid immunology, Transforming Growth Factor beta immunology, Antigens, CD metabolism, Antigens, Differentiation, T-Lymphocyte metabolism, Arthritis, Experimental metabolism, Autoimmunity physiology, Down-Regulation, Transforming Growth Factor beta metabolism
- Abstract
CD69 is induced after activation of leukocytes at inflammatory sites, but its physiological role during inflammation remains unknown. We explored the role of CD69 in autoimmune reactivity by analyzing a model of collagen-induced arthritis (CIA) in WT and CD69-deficient mice. CD69-/- mice showed higher incidence and severity of CIA, with exacerbated T and B cell immune responses to type II collagen. Levels of TGF-beta1 and TGF-beta2, which act as protective agents in CIA, were reduced in CD69-/- mice inflammatory foci, correlating with the increase in the proinflammatory cytokines IL-1beta and RANTES. Local injection of blocking anti-TGF-beta antibodies increased CIA severity and proinflammatory cytokine mRNA levels in CD69+/+ but not in CD69-/- mice. Moreover, in vitro engagement of CD69 induced total and active TGF-beta1 production in Concanavalin A-activated splenocyte subsets, mouse and human synovial leukocytes, and Jurkat stable transfectants of human CD69 but not in the parental CD69 negative cell line. Our results show that CD69 is a negative modulator of autoimmune reactivity and inflammation through the synthesis of TGF-beta, a cytokine that in turn downregulates the production of various proinflammatory mediators.
- Published
- 2003
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41. Enhanced antitumor immunity in mice deficient in CD69.
- Author
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Esplugues E, Sancho D, Vega-Ramos J, Martínez C, Syrbe U, Hamann A, Engel P, Sánchez-Madrid F, and Lauzurica P
- Subjects
- Animals, Antigens, CD genetics, Antigens, Differentiation, T-Lymphocyte genetics, Homeostasis, Killer Cells, Natural immunology, Lectins, C-Type, Mice, Mice, Inbred C57BL, T-Lymphocytes immunology, Transforming Growth Factor beta immunology, Transforming Growth Factor beta physiology, Up-Regulation, Antigens, CD immunology, Antigens, Differentiation, T-Lymphocyte immunology, Neoplasms, Experimental immunology
- Abstract
We investigated the in vivo role of CD69 by analyzing the susceptibility of CD69-/- mice to tumors. CD69-/- mice challenged with MHC class I- tumors (RMA-S and RM-1) showed greatly reduced tumor growth and prolonged survival compared with wild-type (WT) mice. The enhanced anti-tumor response was NK cell and T lymphocyte-mediated, and was due, at least in part, to an increase in local lymphocytes. Resistance of CD69-/- mice to MHC class I- tumor growth was also associated with increased production of the chemokine MCP-1, diminished TGF-beta production, and decreased lymphocyte apoptosis. Moreover, the in vivo blockade of TGF-beta in WT mice resulted in enhanced anti-tumor response. In addition, CD69 engagement induced NK and T cell production of TGF-beta, directly linking CD69 signaling to TGF-beta regulation. Furthermore, anti-CD69 antibody treatment in WT mice induced a specific down-regulation in CD69 expression that resulted in augmented anti-tumor response. These data unmask a novel role for CD69 as a negative regulator of anti-tumor responses and show the possibility of a novel approach for the therapy of tumors.
- Published
- 2003
- Full Text
- View/download PDF
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