1. Microsatellite Marker Discovery in the Stingless Bee Uruçu-Amarela (Melipona rufiventris Group, Hymenoptera, Meliponini) for Population Genetic Analysis
- Author
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Geice Ribeiro da Silva, Maria Teresa R Lopes, Bruno A Souza, Tânia M. Fernandes-Salomão, Helder Canto Resende, F. M. Diniz, Francisca Andréa da Silva Oliveira, Rodrigo Maggioni, Aline Barbosa Negreiros, Fabia M Pereira, ALINE B. NEGREIROS, UFPI, GEICE R. SILVA, UFPI, FRANCISCA A. S. OLIVEIRA, UFC, HELDER C. RESENDE, UFV, TÂNIA M. FERNANDES-SALOMÃO, UFV, RODRIGO MAGGIONI, UFC, FABIA DE MELLO PEREIRA, CPAMN, BRUNO DE ALMEIDA SOUZA, CPAMN, MARIA TERESA DO REGO LOPES, CPAMN, and FABIO MENDONCA DINIZ, CNPC.
- Subjects
Species complex ,molecular markers ,Stingless bee ,Population genetics ,Population ,Zoology ,Biology ,Genetic differentiation ,Marcador Molecular ,Article ,Illumina platform ,Genetic drift ,Diferenciação genética ,education ,education.field_of_study ,Genetic diversity ,Conservação ,conservation ,population genetics ,NGS technology ,biology.organism_classification ,Genetic divergence ,Insect Science ,Microsatellite ,genetic differentiation ,Genética populacional - Abstract
The species Melipona rufiventris Lepeletier, 1836 is a Brazilian native stingless bee that is part of a species complex known as the &lsquo, rufiventris group&rsquo, making it difficult to distinguish between the different species. Populations in this group are facing a severe decline, leading to the risk of local extinction, and therefore, their conservation should be treated as a major concern. This study describes the first set of tri- and tetranucleotide microsatellite markers, using next-generation sequencing technology for use in the identification of genetic diversity and population structure in the &lsquo, A total of 16 microsatellite loci displayed polymorphism. Analysis of the whole data set (n = 50) detected 63 alleles in all loci, ranging from 2 to 7 with a mean of 3.9 alleles/locus. A genetic diversity analysis revealed high values for population differentiation estimates (FST = 0.252, RST = 0.317, and DEST = 0.284) between the Atlantic Forest, Cerrado, and Caatinga biomes. An additional evidence for genetic divergence among populations was also found in the &rsquo, these should be treated as separate conservation units or even as separate species. These microsatellite markers have demonstrated a strong potential for assessing population discrimination in this threatened stingless bee group.
- Published
- 2019