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The application of ecological theory in urban planning is becoming more important as land managers focus on increasing biodiversity to improve human welfare in cities. Authorities must decide not only what types of biodiversity-focused infrastructure should be prioritized, but also where new resources should be positioned and existing resources protected or enhanced. Measuring the contribution of green infrastructure to landscape connectivity can maximise the successful return and conservation of urban nature. By using ecological connectivity theory as a planning tool, the effect of different interventions (both positive and negative) on the ease with which wildlife can move across the landscape can be compared. Here we outline an approach to a) quantify ecological connectivity for different urban wildlife species and b) use this to test different urban planning scenarios using QGIS. We demonstrate extensions which improve the application of this method as a planning tool: • Conversion of the effective mesh size value (meff) to a “probability of connectedness” (Pc, for easier interpretation by local government and comparisons between planning scenarios). • An approach for measuring species-specific connectivity, including how to decide what spatial information should be included and which types of species might be most responsive to connectivity planning. • Guidance for using the method to compare different urban planning scenarios.

Abstract Background Domesticated pigs are the main source of Trichinella sp. infections for humans, particularly when reared in backyards or free-ranging. In temperate areas of southern Europe, most pigs are farmed under controlled housing conditions, but sows and sometimes fattening pigs have access to outdoors to improve animal welfare. The aim of the present study was to investigate whether outdoor access of breeding pigs farmed under controlled housing conditions can represent a risk for Trichinella sp. transmission when the farm is located in an agricultural area interspersed with wooded areas and badlands, where Trichinella spp. could be present in wildlife. Methods Serum samples were collected from 63 breeding sows and one boar before and after their access to an open fenced area for 2 months and from 84 pigs that never had outdoor access. Samples were screened for anti-Trichinella antibodies by ELISA, and positive sera were confirmed using Western blot (Wb) excretory/secretory antigens. To detect Trichinella sp. larvae, muscle tissues from serologically positive and negative pigs were tested by artificial digestion. Results Thirteen (20.6%) sows and one boar tested positive with both ELISA and Wb. No larvae were detected in muscle samples of serologically positive and serologically negative pigs. Positive serum samples were then tested by Wb using crude worm extract as antigens. The Wb banding pattern displayed was that characteristic of encapsulated species (Trichinella spiralis or Trichinella britovi). Conclusions The detection of anti-Trichinella antibodies without larvae in the pig muscles, supported by epidemiological data, suggests that pigs may have been exposed to T. britovi. This study stresses the importance of instigating monitoring systems at farm level to prevent Trichinella sp. transmission and to investigate, through a landscape parasitological study, the suitability of a site before the planting of a high containment level pig farm in which the sows can have outside access to improve their welfare during pregnancy. Graphical abstract

Abstract Background Domesticated and wild swine play an important role as reservoir hosts of Trichinella spp. and a source of infection for humans. Little is known about the survival of Trichinella larvae in muscles and the duration of anti-Trichinella antibodies in pigs with long-lasting infections. Methods Sixty pigs were divided into three groups of 20 animals and infected with 10,000 larvae of Trichinella spiralis, Trichinella britovi or Trichinella pseudospiralis. Four pigs from each group were sacrificed at 2, 6, 12, 18 and 24 months post-infection (p.i.) and the number of larvae per gram (LPG) of muscles was calculated. Serum samples were tested by ELISA and western blot using excretory/secretory (ES) and crude antigens. Results Trichinella spiralis showed the highest infectivity and immunogenicity in pigs and larvae survived in pig muscles for up to 2 years p.i. In these pigs, the IgG level significantly increased at 30 days p.i. and reached a peak at about 60 days p.i., remaining stable until the end of the experiment. In T. britovi-infected pigs, LPG was about 70 times lower than for T. spiralis at 2 months p.i. and only very few infecting larvae were detected at 6 months p.i., whereas no larvae were detected at 12, 18 and 24 months p.i. At 6 months p.i., degenerated/calcified larvae and cysts were detected in the muscles by trichinoscopy and histology. The IgG pattern showed by T. britovi-infected pigs was similar to that of T. spiralis-infected pigs, although seroconversion occurred some days later. The larval burden of T. pseudospiralis was slightly greater than for T. britovi at 2 months p.i., but no larvae were detected at 6 and 12 months p.i. In T. pseudospiralis-infected pigs, seroconversion occurred slowly, as in T. britovi-infected pigs. The IgG level showed a significant drop at 6 months p.i. and declining to the cut-off value at 12 months p.i. Conclusions The longer survival of T. spiralis in pigs in comparison with the other two species highlights its exceptional dissemination potential. These results provide an explanation of the controversial data collected by parasitological and serological tools in the course of epidemiological investigations.

It is increasingly recognised that urban trees can contribute to reducing stormwater runoff by intercepting and retaining a fraction of rainfall received. What is less studied is the translation of this to reduced pollutant loads being transferred to receiving streams, rivers, and water bodies. In this paper, we assess interception of two tree species (Eucalyptus microcorys and Ulmus procera) in an urban park. These data are used in simple water balance modelling to predict the environmental and economic benefit of reducing nitrogen loads to receiving waterways as a function of reduced runoff volume resulting from rainfall interception by urban trees on public land (21% of the catchment area). We use a highly urbanized catchment in Melbourne, Australia to demonstrate the impact of an urban forest dominated by deciduous trees, evergreen trees or a mixed tree canopy cover. We found that doubling the urban canopy cover in the catchment, while keeping the current mix ratio of deciduous and evergreen trees, could reduce annual runoff volume by 30 mm (92 MLyr−1). Using the prescribed values that developers must pay the local water authority for nitrogen treatment as a condition of new development, we calculate that this would deliver a nitrogen load removal benefit of AUD$ 200/tree. If only deciduous trees are planted, the annual runoff reduction would decrease to 24 mm (73 MLyr−1) and increases to 37 mm (112 MLyr−1) if only evergreen trees are planted. This study highlights both the additional benefits of public street trees and the differences in deciduous and evergreen trees which should be accounted for by policy makers.

Built environment practitioners currently seek options and opportunities to respond to the biodiversity emergency. Biodiversity Inclusive Design (BID) is an approach to design that seeks to foster functional ecological systems, enable species’ persistence within the built environment and (re) connect people with nature. BID can support designers’ quest toward biodiversity positivity. However, design projects that prioritise biodiversity are sparse and are limited to ad hoc initiatives by individual champions rather than being standard practice. Frameworks providing a structured design process to achieve biodiversity positivity already exist, but they can be difficult to find, compare and navigate. Responding to calls to further develop the concept of Biodiversity Inclusive Design, we systematically analyse 15 design frameworks compatible with BID. We explore how existing design frameworks position biodiversity as a client. For each framework, we uncover the underlying rules, ideas, beliefs, design principles and proposed structure of the design process. Through a thematic analysis, we identify re-emerging concepts and themes underpinning BID. Nested within complementary design frameworks, we conclude by positioning BID as a set of parallel processes that specifically explore biodiversitys’ perspectives (needs, preferences) and how they interact with the socio-ecological system to give a voice to biodiversity within the planning and design process. Our paper formalises BID as a practice and identifies three core dimensions of design action and nine design principles.

Abstract Background Cryptosporidium parvum is a major cause of diarrhea in children and ruminants at the earliest stages of life. Maternal antibodies represent the main shield of neonate mammals for most of the infections. Two recombinant antigens (SA35 and SA40), portions of two C. parvum proteins, were tested for their ability to induce immune responses in adult mice and for protection on neonate BALB/c mice born from females immunised by mucosal delivery of both peptides. Methods Adult BALB/c mice were intraperitoneally immunised with SA35 and SA40, separately or mixed, and their immune response was characterised. Furthermore, BALB/c pregnant mice were immunised by mucosal delivery with an SA35/40 mix, before and during pregnancy. Soon after birth, their offspring were infected with two doses (1 × 105 and 5 × 103) of C. parvum oocysts and the parasitic burden was determined at 5 and 9 days post-infection. Results Intraperitoneal immunisation with SA35 and SA40 induced specific IgG and IgG1 in serum, specific IgA in the intestinal mucosa, increase of CD3+/CD4+ and CD30+ cells in splenocytes, which produced IFN-γ. Neonates born from immunised mice and infected with 1 × 105 oocysts showed a significant reduction of oocysts and intestinal forms (23 and 42%, respectively). A reduction of all parasitic forms (96%; P

Abstract Background Trichinellosis is a meat-borne zoonotic disease caused by parasites of the genus Trichinella. To date, 12 taxa have been described. The identification of Trichinella species is crucial in order to identify the possible source of infection, the geographical origin of the parasite and to assess risk of infection for domestic pigs and humans. Specific identification of the etiological agent is not always feasible using direct methods since the source of infection can be untraceable. The aim of this study was to develop a diagnostic tool to infer the causative Trichinella species using western blot patterns of sera derived from infected animal and human hosts. Methods Sera from mice experimentally infected with Trichinella spiralis, Trichinella britovi, Trichinella pseudospiralis and Trichinella papuae were tested by western blot using homologous and heterologous crude worm extracts (CWE) and a highly sensitive detection system based on chemiluminescence. In addition, sera from pigs experimentally infected with T. spiralis, T. britovi and T. pseudospiralis and from patients with confirmed T. spiralis, T. britovi and T. pseudospiralis infections, were also included. Results Sera from mice infected with one Trichinella species reacted with CWE proteins from all four investigated species. Likewise, sera derived from pigs and humans infected with one Trichinella species reacted with CWE proteins from all the three investigated species. Using T. spiralis CWE, sera from T. pseudospiralis-infected hosts yielded a characteristic pattern of reactivity using Wb, which differed to that produced by T. spiralis/T. britovi- or T. papuae-infected host sera. Conclusions The present study suggests that western blot using T. spiralis CWE may be a useful tool to distinguish Trichinella infections caused by T. pseudospiralis from those caused by T. spiralis or T. britovi. This method may support epidemiological investigations, particularly when the source of infection is not traceable.

We undertook the first study systematically evaluating the risk of Anisakis-sensitization in Croatian fish-processing workers and potential genetic susceptibility to anisakiasis. Anti-Anisakis IgE seroprevalence and risk factors for 600 employees of Croatian fish processing facilities and 466 blood donor controls, were assessed by indirect ELISA targeted with: recombinant Ani s 1 and Ani s 7 allergens, an Anisakis crude extract, the commercial ImmunoCAP kit, and questionnaires. Genetic susceptibility to anisakiasis was evaluated by genotypisation of human leukocytes alleles (HLA). Anti-Anisakis seropositive and a fraction of negative subjects were also assessed by ELISA and Western Blot (WB) for IgG seroprevalence to Trichinella spp. Overall, the observed anti-Anisakis seroprevalence inferred by indirect ELISA was significantly higher in fish processing workers (1.8%, 95% CI 0.9-3.3%) compared to the controls (0%, 0-0.8%). Seven out of 11 Ani s 1 and Ani s 7-positives and none of selected 65 negative sera, tested positive on whole-Anisakis extract (ImmunoCAP), whereas Anisakis crude extract ELISA detected 3.9% (2.4-6.0%) seropositives in fish processing workers, three (14%) of which showed IgE reactivity to milk proteins. The highest risk associated with Anisakis-sensitization among workers was fishing in the free time, rather than any of attributes related to the occupational exposure. Although no association was observed between anti-Anisakis seropositivity and wearing gloves or protective goggles, the majority of workers (92%) wore protective gloves, minimizing the risk for Anisakis sensitization via skin contact. Six HLA alleles within DRB1 gene were significantly associated with seropositivity under dominant, allelic or recessive models. All sera confirmed negative for anti-Trichinella spp. IgG. The study exhaustively covered almost all marine fish processing workers in Croatia, reflecting real-time Anisakis sensitization status within the industry, already under the influence of wide array of allergens.

Laboratory tools for diagnosing taeniosis/cysticercosis in non-endemic countries are available; however, there is little data on their performance. To provide information on the sensitivity, specificity, and reproducibility of these tools, inter-laboratory studies were organized within the EU COST-Action CYSTINET (TD1302). Two serological and one coprological Ring Trials (RTs) were organized to test a panel of human-derived sera and stool samples using assays routinely conducted by the participating laboratories to detect Taenia spp. infections. Four Western blots (WBs) and five ELISAs were used by nine laboratories for cysticercosis diagnosis. In the first serological RT, the overall sensitivity was 67.6% (95% CI, 59.1–75.4), whereas specificity was 97% (95% CI, 89.8–99.6). WBs recorded the best accuracy. A second serological RT was organized, to assess the three tests most frequently used during the first RT. Two out of six laboratories performed all the three tests. The overall sensitivity and specificity were 52.8% (95% CI, 42.8–62.7) and 98.1% (95% CI, 93.2–99.7), respectively. Laboratory performance strongly affected test results. Twelve laboratories participated in the coprological RT using conventional microscopy and six laboratories used molecular assays. Traditional diagnosis by microscopy yielded better results than molecular diagnosis. This may have been influenced by the lack of standardization of molecular tests across participating laboratories.

Estimation of urban tree canopy parameters plays a crucial role in urban forest management. Unmanned aerial vehicles (UAV) have been widely used for many applications particularly forestry mapping. UAV-derived images, captured by an onboard camera, provide a means to produce 3D point clouds using photogrammetric mapping. Similarly, small UAV mounted light detection and ranging (LiDAR) sensors can also provide very dense 3D point clouds. While point clouds derived from both photogrammetric and LiDAR sensors can allow the accurate estimation of critical tree canopy parameters, so far a comparison of both techniques is missing. Point clouds derived from these sources vary according to differences in data collection and processing, a detailed comparison of point clouds in terms of accuracy and completeness, in relation to tree canopy parameters using point clouds is necessary. In this research, point clouds produced by UAV-photogrammetry and -LiDAR over an urban park along with the estimated tree canopy parameters are compared, and results are presented. The results show that UAV-photogrammetry and -LiDAR point clouds are highly correlated with R2 of 99.54% and the estimated tree canopy parameters are correlated with R2 of higher than 95%.

This paper presents the outcomes from a joint research project that aims to develop a smartphone application/online platform to model the most thermally comfortable active transport route to a planned destination using heat information and tree shading (Shadeway). Here, we provide a summary of our systematic review of academic literature and applications from the Google Play and Apple App Store, to identify current knowledge about personal adaptation strategies when navigating travel in cities during high temperatures. The review identifies that there is a lack of attention regarding the use of smartphone applications to address urban thermal comfort for active transport by government and private industry. We then present the initial results of original research from three community focus groups and an online survey that elicited participants’ opinions about Shadeways in the City of Greater Bendigo (CoGB), Australia. The results clearly show the need for better management of Shadeways in CoGB. For example, 52.3% of the routes traveled by participants suffer from either no or poor levels of shading, and 53 of the shaded areas were located along routes that also experience heavy traffic, which can have an adverse effect on perceptions and actual safety. It is expected that this study will contribute to improve understanding of the methods used to identify adaptation strategies to increasingly extreme temperatures.

Abstract Background Trichinella spp. infections in wild boar (Sus scrofa), one of the main sources of human trichinellosis, continue to represent a public health problem. The detection of Trichinella spp. larvae in muscles of wild boar by digestion can prevent the occurrence of clinical trichinellosis in humans. However, the analytical sensitivity of digestion in the detection process is dependent on the quantity of tested muscle. Consequently, large quantities of muscle have to be digested to warrant surveillance programs, or more sensitive tests need to be employed. The use of indirect detection methods, such as the ELISA to detect Trichinella spp. infections in wild boar has limitations due to its low specificity. The aim of the study was to implement serological detection of anti-Trichinella spp. antibodies in meat juices from hunted wild boar for the surveillance of Trichinella spp. infections. Methods Two tests were used, ELISA for the initial screening test, and a specific and sensitive Western blot (Wb) as a confirmatory test. The circulation of anti-Trichinella IgG was determined in hunted wild boar muscle juice samples in 9 provinces of 5 Italian regions. Results From 1,462 muscle fluid samples, 315 (21.5%, 95% C.I. 19.51-23.73) were tested positive by ELISA. The 315 ELISA-positive muscle fluid samples were further tested by Wb and 32 (10.1%, 95% C.I. 7.29-13.99) of these were positive with a final seroprevalence of 2.2% (95% C.I 1.55-3.07; 32/1,462). Trichinella britovi larvae were detected by artificial digestion in muscle tissues of one (0.07%, 95%C.I. 0.01-0.39) out of the 1,462 hunted wild boars. No Trichinella spp. larvae were detected in Wb-negative wild boar. From 2006 to 2012, a prevalence of 0.017% was detected by muscle digestion in wild boar hunted in the whole Italian territory. Conclusions The combined use of both serological methods had a sensitivity 31.4 times higher than that of the digestion (32/1,462 versus 1/1,462), suggesting their potential use for the surveillance of the Trichinella spp. infection in wild boar populations.

Urban trees deliver many ecological services to the urban environment, including reduced runoff generation in storms. Trees intercept rainfall and store part of the water on leaves and branches, reducing the volume and velocity of water that reaches the soil. Moreover, trees modify the spatial distribution of rainwater under the canopy. However, measuring interception parameters is a complex task because it depends on many factors, including environmental conditions (rainfall intensity, wind speed, etc.) and tree characteristics (plant surface area, leaf and branch inclination angle, etc.). In the few last decades, remotely sensed data have been tested for retrieving tree metrics, but the use of this derived data for predicting interception parameters are still being developed. In this study, we measured the minimum water storage capacity (Cmin) and throughfall under the canopies of 12 trees belonging to three different species. All trees had their plant surface metrics calculated: plant surface area (PSA), plant area index (PAI), and plant area density (PAD). Trees were scanned with a mobile terrestrial laser scan (TLS) to obtain their individual canopy point clouds. Point clouds were used to calculate canopy metrics (canopy projected area and volume) and TLS-derived surface metrics. Measured surface metrics were then correlated to derived TLS metrics, and the relationship between TLS data and interception parameters was tested. Additionally, TLS data was used in analyses of throughfall distribution on a sub-canopy scale. The significant correlation between the directly measured surface area and TLS-derived metrics validates the use of the remotely sensed data for predicting plant area metrics. Moreover, TLS-derived metrics showed a significant correlation with a water storage capacity parameter (Cmin). The present study supports the use of TLS data as a tool for measuring tree metrics and ecosystem services such as Cmin; however, more studies to understand how to apply remotely sensed data into ecological analyses in the urban environment must be encouraged.

Since opisthorchiasis does not show pathognomonic signs or symptoms, physicians can have serious problems to make a differential diagnosis of this infection in non endemic areas, in particular when there is a simultaneous occurrence with other seasonal infections. Moreover, symptomatic infections due to O. felineus can last a few weeks and then the signs and symptoms disappear, but the worms survive in the bile ducts for years causing hepatobiliary diseases including hepatomegaly, cholangitis, fibrosis of the periportal system, cholecystitis, and gallstones. Consequently, an early diagnosis prevents chronicity and loss of working days. The detection of specific antibodies has been considered as a complementary tool to the fecal examination to establish the definitive diagnosis of this infection and for the follow up. Therefore the aim of this work was the development and validation of an enzyme-linked immunosorbent assay (ELISA) using excretory/secretory antigens (ESA) from O. felineus adult worms to detect anti-Opisthorchis IgG in human sera. A total of 370 human sera were tested: 144 sera from persons with a confirmed diagnosis of opisthorchiasis, 110 sera from healthy Italian people, and 116 sera from people with other parasitic or non-parasitic infections. Results were analyzed by receiver-operator characteristic (ROC) curve analysis. The accuracy of the test, calculated by the area under curve (AUC), yielded a 0.999 value, indicating the high performance of the test. The sensitivity was 100% (95% CI: 97.40% to 100%) and no false-negative sera were detected; the specificity was 99.09% (95% CI: 95.02% to 99.83%). The validated ELISA shows a good performance in terms of sensitivity, repeatability and reproducibility, and it is suitable to detect anti-Opisthorchis IgG in human sera for diagnostic purposes and for the follow up to assess the efficacy of drug treatment.

The successful establishment of the greenbelt around London during the 1930s acted as an inspiration for postwar planners in the United Kingdom and in other countries. The history of ideas that supported this establishment has been investigated, but no research has examined the implementation of the 1930s' greenbelt. The objective of this article is to investigate why the greenbelt was successfully established in the 1930s, despite the United Kingdom's weak planning system and the unprecedented urban growth at the time. In particular, the authors highlight the role of landowners and county council members in establishing the greenbelt. Finally, they discuss how the history of the greenbelt has to be reassessed. [ABSTRACT FROM AUTHOR]