35 results on '"Petit, Aurélie"'
Search Results
2. Airway epithelial type‐2 alarmin profiles: Blood eosinophil counts remain in memory.
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Vernisse, Charlotte, Tuaillon, Edouard, Suehs, Carey, Gras, Delphine, Bedin, Anne Sophie, Charriot, Jeremy, Knabe, Lucie, Vachier, Isabelle, Chanez, Pascal, Petit, Aurélie, and Bourdin, Arnaud
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THYMIC stromal lymphopoietin ,EOSINOPHILS ,CHRONIC obstructive pulmonary disease - Abstract
Epithelial cytokines are involved in the orchestration of T1/T2 inflammatory patterns. We question the persistence of this trait in air–liquid interface (ALI) epithelial cultures and whether this local orientation can be related to systemic patterns (e.g., blood eosinophil counts [BECs]). We investigated alarmin release related to high versus low T2 phenotypes associated with chronic airway diseases. ALIs were reconstituted from 32 control, 40 chronic obstructive pulmonary disease, and 20 asthmatic patients. Interleukin‐8 (IL‐8; a T1‐cytokine), IL‐25, IL‐33, and thymic stromal lymphopoietin (T2‐alarmins) concentrations were assessed in subnatants at steady state and used to explain blood neutrophil and eosinophil counts. IL‐25 and IL‐8 levels were highest in asthma ALI‐subnatants, whereas IL‐33 was sparsely detected. Thymic stromal lymphopoietin levels were similar among groups. All asthma cell cultures were T1‐high/T2‐high, while chronic obstructive pulmonary disease and controls tended to be mixed. BECs were independently explained by both disease and in‐culture T2‐alarmin levels, irrespective of the T2‐alarmin considered. The epithelial ALI‐T2 signature was more frequently high in patients with a BEC > 300/mm3. Despite removal from an in vivo environment for ≥2 months, ALIs release disease‐specific cytokine "cocktails" into their subnatants, suggesting continued persistence of alarmin orientation in differentiated cell line environments. [ABSTRACT FROM AUTHOR]
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- 2023
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3. Generation of a healthy heavy smoker patient-derived induced pluripotent stem cell line UHOMi007-A from peripheral blood mononuclear cells
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Ahmed, Engi, Fieldès, Mathieu, Bourguignon, Chloé, Mianné, Joffrey, Petit, Aurélie, Amel, Nasri, Foisset, Florent, Bourdais, Carine, Vachier, Isabelle, Assou, Said, De Vos, John, and Bourdin, Arnaud
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- 2024
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4. PFRU, a single dominant locus regulates the balance between sexual and asexual plant reproduction in cultivated strawberry
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Gaston, Amèlia, Perrotte, Justine, Lerceteau-Köhler, Estelle, Rousseau-Gueutin, Mathieu, Petit, Aurélie, Hernould, Michel, Rothan, Christophe, and Denoyes, Béatrice
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- 2013
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5. "Do female anime fans exist?" The impact of women-exclusionary discourses on rec.arts.anime.
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Petit, Aurélie
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ANIME , *INTERNET forums , *VIRTUAL communities , *DISCURSIVE practices , *POISONS - Abstract
This paper examines women-exclusionary discourses on the popular anime Usenet newsgroup, rec.arts.anime. By going back to pre-2000 online anime histories, this paper proposes to understand how women-exclusionary discursive practices on rec.arts.anime have contributed to shaping contemporary toxic technocultures' discursive identities, as it is admitted that forum 4chan originated from online anime fandom. By using a data set of 252 messages related to gender issues posted from 1992 to 1996, I identify 7 discursive practices that I am theorizing here under the name of negative networking: 1. Blaming female anime fans for their lack of visibility; 2. Doubting the authentic interest of women in anime; 3. Mystifying the female anime fan; 4. Harassing female anime fans; 5. Criticizing the association of feminism with anime, both as interpretive practices and as scholarship; 6. Belittling female anime fans' concerns; and 7. Denying or ignoring the challenges faced by female anime fans. I argue that the impact of these discourses must be understood as determinant in the establishment of the online anime hegemonic fan identity and its prediscourses, especially as they relate to the long-lasting marginalization of women and gender diverse anime fans. [ABSTRACT FROM AUTHOR]
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- 2022
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6. Supplementing Defect in Club Cell Secretory Protein Attenuates Airway Inflammation in COPD
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Gamez, Anne Sophie, Gras, Delphine, Petit, Aurélie, Knabe, Lucie, Molinari, Nicolas, Vachier, Isabelle, Chanez, Pascal, and Bourdin, Arnaud
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- 2015
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7. Differentiation of Human Induced Pluripotent Stem Cells from Patients with Severe COPD into Functional Airway Epithelium.
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Ahmed, Engi, Fieldes, Mathieu, Bourguignon, Chloé, Mianné, Joffrey, Petit, Aurélie, Jory, Myriam, Cazevieille, Chantal, Boukhaddaoui, Hassan, Garnett, James P., Hirtz, Christophe, Massiera, Gladys, Vachier, Isabelle, Assou, Said, Bourdin, Arnaud, and De Vos, John
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PLURIPOTENT stem cells ,INDUCED pluripotent stem cells ,LUNGS ,EPITHELIUM ,MONONUCLEAR leukocytes ,CHRONIC obstructive pulmonary disease ,DRUG discovery ,NEUROENDOCRINE cells - Abstract
Background: Chronic Obstructive Pulmonary Disease (COPD), a major cause of mortality and disability, is a complex disease with heterogeneous and ill-understood biological mechanisms. Human induced pluripotent stem cells (hiPSCs) are a promising tool to model human disease, including the impact of genetic susceptibility. Methods: We developed a simple and reliable method for reprogramming peripheral blood mononuclear cells into hiPSCs and to differentiate them into air–liquid interface bronchial epithelium within 45 days. Importantly, this method does not involve any cell sorting step. We reprogrammed blood cells from one healthy control and three patients with very severe COPD. Results: The mean cell purity at the definitive endoderm and ventral anterior foregut endoderm (vAFE) stages was >80%, assessed by quantifying C-X-C Motif Chemokine Receptor 4/SRY-Box Transcription Factor 17 (CXCR4/SOX17) and NK2 Homeobox 1 (NKX2.1) expression, respectively. vAFE cells from all four hiPSC lines differentiated into bronchial epithelium in air–liquid interface conditions, with large zones covered by beating ciliated, basal, goblets, club cells and neuroendocrine cells, as found in vivo. The hiPSC-derived airway epithelium (iALI) from patients with very severe COPD and from the healthy control were undistinguishable. Conclusions: iALI bronchial epithelium is ready for better understanding lung disease pathogenesis and accelerating drug discovery. [ABSTRACT FROM AUTHOR]
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- 2022
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8. Using intracellular SCGB1A1-sorted, formalin-fixed club cells for successful transcriptomic analysis.
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Vernisse, Charlotte, Petit, Aurélie, Pantesco, Véronique, Chanez, Pascal, Gras, Delphine, Tuaillon, Edouard, Duperray, Christophe, Vachier, Isabelle, Assou, Said, and Bourdin, Arnaud
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TRANSCRIPTOMES , *RIBONUCLEASES , *SAPONINS , *FLOW cytometry , *RNA , *CLUBS - Abstract
As opposed to surface marker staining, certain cell types can only be recognized by intracellular markers. Intracellular staining for use in cell sorting remains challenging. Fixation and permeabilization steps for intracellular staining and the presence of RNases notably affect preservation of high-quality mRNA. We report the work required for the optimization of a successful protocol for microarray analysis of intracellular target-sorted, formalin-fixed human bronchial club cells. Cells obtained from differentiated air-liquid interface cultures were stained with the most characteristic intracellular markers for club cell (SCGB1A1+) sorting. A benchmarked intracellular staining protocol was carried out before flow cytometry. The primary outcome was the extraction of RNA sufficient quality for microarray analysis as assessed by Bioanalyzer System. Fixation with 4% paraformaldehyde coupled with 0.1% Triton/0.1% saponin permeabilization obtained optimal results for SCGB1A1 staining. Addition of RNase inhibitors throughout the protocol and within the appropriate RNA extraction kit (Formalin-Fixed-Paraffin-Embedded) dramatically improved RNA quality, resulting in samples eligible for microarray analysis. The protocol resulted in successful cell sorting according to specific club cell intracellular marker without using cell surface marker. The protocol also preserved RNA of sufficient quality for subsequent microarray transcriptomic analysis, and we were able to generate transcriptomic signature of club cells. • Successful SCGB1A1 sorting according to specific intracellular marker. • Preserved RNA of sufficient quantity and quality for microarray experiment. • Pathways underlying both mucus production and ciliogenesis in SCGB1A1+ cells. • Signature of basal cells for SCGB1A1- cells. [ABSTRACT FROM AUTHOR]
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- 2022
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9. Bronchial epithelium as a target for innovative treatments in asthma
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Gras, Delphine, Chanez, Pascal, Vachier, Isabelle, Petit, Aurélie, and Bourdin, Arnaud
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- 2013
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10. High Resolution Quantitative Trait Locus Mapping and Whole Genome Sequencing Enable the Design of an Anthocyanidin Reductase -Specific Homoeo-Allelic Marker for Fruit Colour Improvement in Octoploid Strawberry (Fragaria × ananassa).
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Labadie, Marc, Vallin, Guillaume, Potier, Aline, Petit, Aurélie, Ring, Ludwig, Hoffmann, Thomas, Gaston, Amèlia, Munoz-Blanco, Juan, Caballero, José L., Schwab, Wilfried, Rothan, Christophe, and Denoyes, Béatrice
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STRAWBERRIES ,LOCUS (Genetics) ,WHOLE genome sequencing ,GENE mapping ,FRUIT ,COLOR of fruit - Abstract
Fruit colour is central to the sensorial and nutritional quality of strawberry fruit and is therefore a major target in breeding programmes of the octoploid cultivated strawberry (Fragaria × ananassa). The red colour of the fruit is caused by the accumulation of anthocyanins, which are water-soluble flavonoids. To facilitate molecular breeding, here we have mapped with high resolution fruit colour quantitative trait loci (QTLs) (COLOUR, scored visually as in selection programmes) and associated flavonoid metabolic QTLs (5 anthocyanins compounds together with 8 flavonols and flavan-3-ols) to specific subgenomes of cultivated strawberry. Two main colour-related QTLs were located on the LG3A linkage group (Fragaria vesca subgenome). Genetic mapping, transcriptome analysis and whole genome sequencing enabled the detection of a homoeo-allelic variant of ANTHOCYANIDIN REDUCTASE (ANR) underlying the major male M3A COLOUR and pelargonidin-3-glucoside (PgGs) QTLs (up to ∼20% of explained variance). Consistent with previously published functional studies, ANR transcript abundance was inversely related with PgGs content in contrasted progeny individuals. Genetic segregation analyses further indicated that a molecular marker designed using an 18 bp deletion found in the 5′UTR of the candidate ANR homoeo-allelic variant is effective in identifying genotypes with intense red fruit colour. Our study provides insights into the genetic and molecular control of colour-related traits in strawberry and further defines a genetic marker for marker-assisted selection of new strawberry varieties with improved colour. The QTLs detected and the underlying candidate genes are different from those described to date, emphasising the importance of screening a wide diversity of genetic resources in strawberry. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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11. Deciphering the balance between sexual and asexual plant reproduction in an herbaceous perennial, the strawberry. [Poster-D268]
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Gaston, Amèlia, Potier, A., Tenreira, T., Labadie, Marc, Petit, Aurélie, Bres, Cécile, Perrotte, Justine, Demené, Marie-Noëlle, Guédon, Yann, Rothan, Christophe, Hernould, Michel, and Delmas, Frédéric
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fungi ,food and beverages - Abstract
Strawberry, the major berry fruit crop, is a relevant model to study the occurrence and concomitance of various developmental processes/ events . Vegetative reproduction through stolons (elongated stems) and sexual reproduction through inflorescences occur successively or jointly, depending on the genotype, cultural technique and environment. The control of the trade-off between flowering and runnering is crucial for managing the production of plants in nurseries and the production of fruits in fields, tunnels or greenhouses. The runnering and flowering pathways likely interact. We recently showed in diploid strawberry that the gibberellin biosynthetic enzyme GA20ox plays a key role in controlling this trade-off by regulating the fate of axillary meristem to either stolon or inflorescence. We also showed that the floral repressor FvTFL1 controls perpetual flowering (PF). Interestingly, in the cultivated octoploid strawberry, a single major locus FaPFRU that does not involve GA20ox or TFL1 controls PF and displays opposite effects on flowering and runnering. We further identified by using longitudinal data analysis methods a locus controlling the flowering intensity of the last phase of PF. We will present how these results deepen our understanding of the underlying mechanisms and allow a better control of fruit yield in strawberry.
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- 2018
12. Application of multiomic technologies to study the environmental impact on berry fruit quality
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Jimenez, Lidia, Pott, Delphine, Duran, Sara, Mott, Daniella, Petit, Aurélie, Celejewska, Karolina, Piecko, Jan, Masny, Agnieska, Savini, Gianluca, Chartier, Philippe, Krüger, Erika, Sonsteby, Anita, Denoyes, Beatrice, Vallarino, José G., and Osorio-Algar, Sonia
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Fruit quality ,Climate changes ,Metabolomic ,Strawberry ,Agricultura -- Innovaciones tecnológicas - Abstract
Berries, such as strawberry, raspberry and black currant, are well appreciated for their delicate flavor and nutraceutical properties, with consumer demand increasing over the last years. However, climate change and market globalization have made necessary to improve the production while maintaining fruit quality traits. Among the EU GoodBerry project’s objetive are develop state-of-the-art analytical platforms, covering from transcriptomic to metabolites and volatile compounds analysis, to find new factors controlling plant adaptation, fruit production and quality and use the data to face climate changes. Here we present the metabolomic analysis of strawberry, raspberry and black currant fruits from the 2017 harvest. Different berry cultivars, adapted to diverse environments, were grown in 2017 and 2018 in different latitudes (Germany, France, Norway, Italy and Poland) combination of spectrometry techniques was used to semi-quantify fruit primary metabolome and volatilome. Around 50 key primary metabolites, including sugars and acids, which are fundamental factors influencing fruit taste and 75 volatiles, responsible of the aroma, were identified across the different genotypes and climates. Multivariate statistical approaches allow us to point out the genetic and environmental factors underlying complex metabolic traits involved in fruit quality. Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.
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- 2018
13. Influence of Post-Flowering Climate Conditions on Anthocyanin Profile of Strawberry Cultivars Grown from North to South Europe.
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Krüger, Erika, Will, Frank, Kumar, Keshav, Celejewska, Karolina, Chartier, Philippe, Masny, Agnieszka, Mott, Daniela, Petit, Aurélie, Savini, Gianluca, and Sønsteby, Anita
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ANTHOCYANINS ,STRAWBERRIES ,GLOBAL radiation ,PRINCIPAL components analysis ,CULTIVARS ,FRUIT growing ,REGRESSION analysis - Abstract
The effect of cultivar and environmental variations and their interaction on anthocyanin components of strawberry were assessed for six cultivars grown in five locations from North to South of Europe in two different years. To evaluate the impact of latitude- and altitude-related factors, daily mean (T
mean ), maximum (Tmax ) and minimum (Tmin ) temperature and global radiation accumulated for 3, 5, 10 and 15 days before fruit sampling, was analyzed. In general, fruits grown in the south were more enriched in total anthocyanin and pelargonidin-3-glucoside (pel-3-glc), the most abundant anthocyanin in strawberry. Principal component analysis (PCA) provided a separation of the growing locations within a cultivar due to latitudinal climatic differences, temporary weather changes before fruit collection and cultivation technique. PCA also depicted different patterns for anthocyanin distribution indicating a cultivar specific reaction on the environmental factors. The linear regression analysis showed that pel-3-glc was relatively less affected by these factors, while the minor anthocyanins cyanidin-3-glucoside, cyanidin-3-(6-O-malonyl)-glucoside, pelargonidin-3-rutinoside and pelargonidin-3-(6-O-malonoyl)-glucoside were sensitive to Tmax . The global radiation strongly increased cya-3-mal-glc in 'Frida' and pel-3-rut in 'Frida' and 'Florence'. 'Candonga' accumulated less pel-3-glc and total anthocyanin with increased global radiation. The anthocyanin profiles of 'Gariguette' and 'Clery' were unaffected by environmental conditions. [ABSTRACT FROM AUTHOR]- Published
- 2021
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14. Metabolite Quantitative Trait Loci for Flavonoids Provide New Insights into the Genetic Architecture of Strawberry (Fragaria × ananassa) Fruit Quality.
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Labadie, Marc, Vallin, Guillaume, Petit, Aurélie, Ring, Ludwig, Hoffmann, Thomas, Gaston, Amèlia, Potier, Aline, Schwab, Wilfried, Rothan, Christophe, and Denoyes, Béatrice
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- 2020
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15. QualiFraise: An Ambitious Research Project To Improve The Stability Of Strawberry Fruit Quality
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PETIT , Aurélie, Carmagnat, Christophe, Perrotte, Justine, Demene, Marie-Noëlle, Marais, Armelle, DENOYES, Beatrice, Delmas, Frédéric, Benhamed, Moussa, Ziessel, Julie, Création Variétale Fraises Fruits Rouges (Ciref), Centre Interrégional de Recherche et d'Expérimentation de la Fraise (CIREF), Fruits et Légumes d'Aquitaine (INVENIO), Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, Université Paris Saclay (COmUE), Administration - Ingénieurie - Ressources - Expertises fruits et légumes, Partenaires INRAE, and ProdInra, Migration
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project ,misshapen fruit ,epigenetics ,fruit quality ,physiology ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,[SDV.BV] Life Sciences [q-bio]/Vegetal Biology ,genetics ,strawberry ,ComputingMilieux_MISCELLANEOUS ,virology - Abstract
International audience
- Published
- 2016
16. Narrowing down the single homoeologous FaPFRU locus controlling flowering in cultivated octoploid strawberry using a selective mapping strategy
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Perrotte, Justine, Gaston, Amèlia, Potier, Aline, Petit, Aurélie, Denoyes, Béatrice, Petit, Johann, Bres, Cécile, Mauxion, Jean-Philippe, Wong Jun Tai, Fabienne, Martin, Laetitia B.B., Fich, Eric, Joubès, Jérôme, Rose, Jocelyn K.C., Domergue, Frédéric, Rothan, C., Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, Création Variétale Fraises Fruits Rouges (Ciref), Centre Interrégional de Recherche et d'Expérimentation de la Fraise (CIREF), Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Laboratoire de l'intégration, du matériau au système (IMS), Université Sciences et Technologies - Bordeaux 1-Institut Polytechnique de Bordeaux-Centre National de la Recherche Scientifique (CNRS), Laboratoire de biogenèse membranaire (LBM), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS), and Cornell University [New York]
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0301 basic medicine ,[SDV.SA]Life Sciences [q-bio]/Agricultural sciences ,Genetic Linkage ,[SDV]Life Sciences [q-bio] ,Population ,Locus (genetics) ,Plant Science ,Flowers ,Biology ,Fragaria ,03 medical and health sciences ,Polyploid ,bin mapping ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,perpetual flowering ,Allele ,education ,Research Articles ,ComputingMilieux_MISCELLANEOUS ,polyploidy ,Plant Proteins ,2. Zero hunger ,Genetics ,education.field_of_study ,fungi ,food and beverages ,Marker-assisted selection ,030104 developmental biology ,Trait ,marker assisted selection ,marker‐assisted selection ,Ploidy ,Agronomy and Crop Science ,Genome, Plant ,Biotechnology ,Research Article - Abstract
International audience; Extending the period of fruit production is a way to substantially increase crop yield in many fruit or ornamental species. In the cultivated octoploid strawberry (Fragaria x ananassa), the most consumed small fruit worldwide, fruit production season can be extended by selecting perpetual flowering (PF) cultivars. This trait is of considerable interest to growers and to the food industry. Four homoeologous loci controlling a single trait can be expected in such a complex octoploid species. However, we recently showed that the PF trait is under the control of the single dominant FaPFRU locus (Gaston et al., 2013), making it potentially amenable to marker assisted selection (MAS). Here, we report the successful use of a strategy, based on selective mapping using a reduced sample of individuals, to identify nine markers in close linkage to the FaPFRU allelic variant. Thus, this strategy can be used to fine map target homoeologous loci in other complex polyploid crop species. Recombinant analysis further enabled us to reduce the locus to a region flanked by two markers, Bx083_206 and Bx215_131, corresponding to a 1.1 Mb region in the diploid F. vesca reference genome. This region comprised 234 genes, including 15 flowering-associated genes. Among these, the FLOWERING LOCUS T (FT) is known to be a key activator of flowering. The close association between the PF trait and the FaPFRU flanking markers was validated using an additional segregating population and genetic resources. This study lays the foundation for effective and rapid breeding of PF strawberry cultivars by MAS. This article is protected by copyright. All rights reserved.
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- 2016
17. Combined genetic, genomic and physiological approaches to characterize flowering in Fragaria [W324]
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Gaston, Amèlia, Perrotte, Justine, Tenreira, T., Demené, Marie-Noëlle, Potier, A., Petit, Aurélie, Guédon, Yann, Godin, Christophe, Rothan, Christophe, and Denoyes, Béatrice
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F63 - Physiologie végétale - Reproduction ,F30 - Génétique et amélioration des plantes - Abstract
Flowering is a key event for production of seeds or fruits. To date, studies of this process were mainly focused on the molecular mechanisms involved in the control of flowering until now. In perennial plants, few studies have taken into account the large variability in flowering patterns along plant development. Fragaria stands as an interesting model for studying flowering and perpetual flowering and its relationships with vegetative plant reproduction in perennial plants. In this talk, we will show how complementary approaches in genetics, genomics and plant physiology can be integrated to provide a better understanding of flowering in Fragaria. Besides giving a better insights into these poorly known processes, our results will provide new tools for controlling that trait in strawberry and, consequently, fruit production.
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- 2015
18. The role of MYB factor in fruit strawberry color
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Fouché, Mathieu, Denoyes, Beatrice, Rothan, Christophe, Vignes, Matthieu, Mangin, Brigitte, Petit, Aurélie, Chartier, P., Schwab, W., Monfort, A., Villatoro, C., Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, Unité de Biométrie et Intelligence Artificielle (UBIA), Institut National de la Recherche Agronomique (INRA), CIREF, Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM), Institute of Agrifood Research and Technology (IRTA), and ProdInra, Migration
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QTL ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,[SDV.BV] Life Sciences [q-bio]/Vegetal Biology ,Myb ,strawberry ,ComputingMilieux_MISCELLANEOUS ,Fragaria x ananassa ,fruit color - Abstract
International audience
- Published
- 2012
19. Antioxidants and health benefits of strawberry: a review of current research
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Petit, Aurélie, Chartier, P., Baldet, Pierre, Rothan, Christophe, Denoyes, Beatrice, CIREF, Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, and ProdInra, Migration
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[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,[SDV.BV] Life Sciences [q-bio]/Vegetal Biology ,ComputingMilieux_MISCELLANEOUS - Abstract
National audience
- Published
- 2012
20. Complementary strategies for breeding cultivated strawberry for high level of antioxydants
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PETIT , Aurélie, Tallès, K., Jousseaume, G., Chartier, P., DENOYES, Beatrice, ProdInra, Migration, CIREF, Biologie du fruit et pathologie (BFP), and Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)
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genetic resources ,antioxidant ,QTL ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,[SDV.BV] Life Sciences [q-bio]/Vegetal Biology ,strawberry ,ComputingMilieux_MISCELLANEOUS ,Fragaria x ananassa - Abstract
International audience
- Published
- 2012
21. How strawberry can initiate continuous flowering: the genetic dissection of an agronomical interest trait, continuously-flowering in Fragaria x ananassa
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Gaston-Dauzon, Amélia, Petit, Aurélie, Lerceteau-Köhler, Estelle, Barrot, Laure, Rousseau-Gueutin, Mathieu, Denoyes, Béatrice, UMR INRA / Univ. Bordeaux 1 / Univ. Bordeaux 2 : Physiologie et Biotechnologie Végétales, Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux 1 - Sciences Technologies (U. Bordeaux 1)-Université Victor Segalen - Bordeaux 2 (U. Bordeaux 2), ProdInra, Migration, Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)
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[SDV] Life Sciences [q-bio] ,[SDE] Environmental Sciences ,[SDV]Life Sciences [q-bio] ,[SDE]Environmental Sciences ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,[SDV.BV] Life Sciences [q-bio]/Vegetal Biology ,ComputingMilieux_MISCELLANEOUS - Abstract
International audience
- Published
- 2010
22. Genetic dissection of seasonal vs recurrent flowering for better management of the production of fruits in the cultivated strawberry
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Gaston, Amèlia, Lerceteau-Köhler, Estelle, Barreau, Laure, Petit, Aurélie, Schafleitner, Sadia, Rousseau-Gueutin, Mathieu, Denoyes-Rothan, Beatrice, Unité de recherches Espèces Fruitières et Vigne (UREFV), Institut National de la Recherche Agronomique (INRA), and ProdInra, Migration
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[SDV] Life Sciences [q-bio] ,[SDV]Life Sciences [q-bio] ,FRAISIER ,CARTOGRAPHIE GENETIQUE ,PRODUCTION DU FRUIT ,ComputingMilieux_MISCELLANEOUS - Abstract
National audience
- Published
- 2008
23. Club cell secretory protein serum concentration is a surrogate marker of small-airway involvement in asthmatic patients.
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Bommart, Sebastien, Marin, Grégory, Molinari, Nicolas, Knabe, Lucie, Petit, Aurélie, Chanez, Pascal, Gamez, Anne Sophie, Devautour, Catherine, Vachier, Isabelle, and Bourdin, Arnaud
- Published
- 2017
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24. The HDAC inhibitor SAHA does not rescue CFTR membrane expression in Cystic Fibrosis.
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Bergougnoux, Anne, Petit, Aurélie, Knabe, Lucie, Bribes, Estelle, Chiron, Raphaël, De Sario, Albertina, Claustres, Mireille, Molinari, Nicolas, Vachier, Isabelle, Taulan-Cadars, Magali, and Bourdin, Arnaud
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CYSTIC fibrosis treatment , *CYSTIC fibrosis transmembrane conductance regulator genetics , *HYDROXAMIC acids , *HISTONE deacetylase , *METAPLASIA - Abstract
The development of suitable Cystic Fibrosis (CF) models for preclinical bench tests of therapeutic candidates is challenging. Indeed, the validation of molecules to rescue the p.Phe508del-CFTR channel (encoded by the Cystic Fibrosis Transmembrane conductance Regulator gene carrying the p.Phe508del mutation) requires taking into account their overall effects on the epithelium. Suberoylanilide Hydroxamic Acid (SAHA), a histone deacetylase inhibitor (HDACi), was previously shown to be a CFTR corrector via proteostasis modulation in CFTR-deficient immortalized cells. Here, we tested SAHA effects on goblet cell metaplasia using an ex vivo model based on the air-liquid interface (ALI) culture of differentiated airway epithelial cells obtained by nasal scraping from CF patients and healthy controls. Ex vivo epithelium grew successfully in ALI cultures with significant rise in the expression of CFTR and of markers of airway epithelial differentiation compared to monolayer cell culture. SAHA decreased CFTR transcript and protein levels in CF and non-CF epithelia. Whereas SAHA induced lysine hyperacetylation, it did not change histone modifications at the CFTR promoter. SAHA reduced MUC5AC and MUC5 B expression and inhibited goblet epithelial cell differentiation. Similar effects were obtained in CF and non-CF epithelia. All the effects were fully reversible within five days from SAHA withdrawal. We conclude that, ex vivo, SAHA modulate the structure of airway epithelia without specific effect on CFTR gene and protein suggesting that HDACi cannot be useful for CF treatment. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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25. Epithelial ciliated beating cells essential for ex vivo ALI culture growth.
- Author
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Gras, Delphine, Petit, Aurélie, Charriot, Jérémy, Knabe, Lucie, Alagha, Khuder, Gamez, Anne Sophie, Garulli, Céline, Bourdin, Arnaud, Chanez, Pascal, Molinari, Nicolas, and Vachier, Isabelle
- Subjects
EPITHELIAL cells ,NATURAL immunity ,LUNG biopsy ,OBSTRUCTIVE lung diseases ,EPITHELIUM ,BRONCHI ,CELL survival ,ASTHMA ,BRONCHOSCOPY ,CELL culture ,CULTURE media (Biology) ,LONGITUDINAL method ,MULTIVARIATE analysis ,RESPIRATORY mucosa ,SMOKING ,LOGISTIC regression analysis ,PROPORTIONAL hazards models ,SEVERITY of illness index ,CASE-control method ,CELL physiology - Abstract
Background: Bronchial epithelium plays a key role in orchestrating innate and adaptive immunity. The fate of ex vivo airway epithelial cultures growing at the air liquid interface (ALI) derived from human endobronchial biopsies or brushings is not easy to predict. Calibrating and differentiating these cells is a long and expensive process requiring rigorous expertise. Pinpointing factors associated with ALI culture success would help researchers gain further insight into epithelial progenitor behavior.Methods: A successful ALI culture was defined as one in which a pseudostratified epithelium has formed after 28 days in the presence of all differentiated epithelial cell types. A 4-year prospective bi-center study was conducted with adult subjects enrolled in different approved research protocols.Results: 463 consecutive endobronchial biopsies were obtained from normal healthy volunteers, healthy smokers, asthmatic patients and smokers with COPD. All demographic variables, the different fiber optic centers and culture operators, numbers of endo-bronchial biopsies and the presence of ciliated cells were carefully recorded. Univariate and multivariate models were developed. A stepwise procedure was used to select the final logistic regression model. ALI culture success was independently associated with the presence of living ciliated cells within the initial biopsy (OR = 2.18 [1.50-3.16], p < 0.001).Conclusion: This finding highlights the properties of the cells derived from the epithelium dedifferentiation process. The preferential selection of samples with ciliated beating cells would probably save time and money. It is still unknown whether successful ALI culture is related to indicators of general cell viability or a purported stem cell state specifically associated with ciliated beating cells. [ABSTRACT FROM AUTHOR]- Published
- 2017
- Full Text
- View/download PDF
26. Narrowing down the single homoeologous Fa PFRU locus controlling flowering in cultivated octoploid strawberry using a selective mapping strategy.
- Author
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Perrotte, Justine, Gaston, Amèlia, Potier, Aline, Petit, Aurélie, Rothan, Christophe, and Denoyes, Béatrice
- Subjects
STRAWBERRY growing ,POLYPLOIDY in plant chromosomes ,CROP yields ,FRUIT ,PLANT breeding ,FRUIT varieties - Abstract
Extending the period of fruit production is a way to substantially increase crop yield in many fruit or ornamental species. In the cultivated octoploid strawberry ( Fragaria × ananassa), the most consumed small fruit worldwide, fruit production season can be extended by selecting the perpetual flowering ( PF) cultivars. This trait is of considerable interest to growers and to the food industry. Four homoeologous loci controlling a single trait can be expected in such a complex octoploid species. However, we recently showed that the PF trait is under the control of the single dominant Fa PFRU locus ( J. Exp. Bot., 2013, 64, 1837), making it potentially amenable to marker-assisted selection ( MAS). Here, we report the successful use of a strategy, based on a selective mapping using a reduced sample of individuals, to identify nine markers in close linkage to the FaPFRU allelic variant. Thus, this strategy can be used to fine map the target homoeologous loci in other complex polyploid crop species. Recombinant analysis further enabled us to reduce the locus to a region flanked by two markers, Bx083_206 and Bx215_131, corresponding to a 1.1 Mb region in the diploid F. vesca reference genome. This region comprises 234 genes, including 15 flowering associated genes. Among these, the FLOWERING LOCUS T ( FT) is known to be a key activator of flowering. The close association between the PF trait and the Fa PFRU flanking markers was validated using an additional segregating population and genetic resources. This study lays the foundation for effective and rapid breeding of PF strawberry cultivars by MAS. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
27. CCSP G38A polymorphism environment interactions regulate CCSP levels differentially in COPD.
- Author
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Knabe, Lucie, Varilh, Jessica, Bergougnoux, Anne, Gamez, Anne-Sophie, Bonini, Jennifer, Pommier, Alexandra, Petit, Aurélie, Molinari, Nicolas, Vachier, Isabelle, Taulan-Cadars, Magali, and Bourdin, Arnaud
- Subjects
GENETIC polymorphisms ,OBSTRUCTIVE lung diseases ,TRANSCRIPTION factors ,CIGARETTE smoke ,BODY mass index ,GENE transfection ,DYSPNEA - Abstract
Impaired airway homeostasis in chronic obstructive pulmonary disease (COPD) could be partly related to club cell secretory protein (CCSP) deficiency. We hypothesize that CCSP G38A polymorphism is involved and aim to examine the influence of the CCSP G38A polymorphism on CCSP transcription levels and its regulatory mechanisms. CCSP genotype and CCSP levels in serum and sputum were assessed in 66 subjects with stable COPD included in a 1-yr observational study. Forty-nine of them had an exacerbation. In an in vitro study, the impact on the CCSP promoter of 38G wild-type or 38A variant was assessed. BEAS-2B cells were transfected by either the 38G or 38A construct, in the presence/absence of cigarette smoke extract (CSE) or lipopolysaccharides (LPS). Cotransfections with modulating transcription factors, p53 and Nkx2.1, identified by in silico analysis by using ConSite and TFSEARCH were conducted. A allele carrier COPD patients had lower serum and sputum CCSP levels, especially among active smokers, and a decreased body mass index, airflow obstruction, dyspnea, and exercise capacity (BODE) score. In vitro, baseline CCSP transcription levels were similar between the wild and variant constructs. CSE decreased more profoundly the CCSP transcription level of 38A transfected cells. The opposite effect was observed with p53 cotransfection. LPS stimulation induced CCSP repression in 38A promoter transfected cells. Cotransfection with Nkx2.1 significantly activated the CCSP promoters irrespective of the polymorphism. Circulating CCSP levels are associated with smoking and the CCSP G38A polymorphism. CSE, LPS, and the Nkx2.1 and p53 transcription factors modulated the CCSP promoter efficiency. The 38A polymorphism exaggerated the CCSP repression in response to p53 and CSE. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
28. Methods of Sputum and Mucus Assessment for Muco-Obstructive Lung Diseases in 2022: Time to "Unplug" from Our Daily Routine!
- Author
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Charriot, Jeremy, Volpato, Mathilde, Petit, Aurélie, Vachier, Isabelle, and Bourdin, Arnaud
- Subjects
MUCUS ,LUNG diseases ,CHRONIC obstructive pulmonary disease ,SPUTUM ,OBSTRUCTIVE lung diseases ,PULMONARY function tests - Abstract
Obstructive lung diseases, such as chronic obstructive pulmonary disease, asthma, or non-cystic fibrosis bronchiectasis, share some major pathophysiological features: small airway involvement, dysregulation of adaptive and innate pulmonary immune homeostasis, mucus hyperproduction, and/or hyperconcentration. Mucus regulation is particularly valuable from a therapeutic perspective given it contributes to airflow obstruction, symptom intensity, disease severity, and to some extent, disease prognosis in these diseases. It is therefore crucial to understand the mucus constitution of our patients, its behavior in a stable state and during exacerbation, and its regulatory mechanisms. These are all elements representing potential therapeutic targets, especially in the era of biologics. Here, we first briefly discuss the composition and characteristics of sputum. We focus on mucus and mucins, and then elaborate on the different sample collection procedures and how their quality is ensured. We then give an overview of the different direct analytical techniques available in both clinical routine and more experimental settings, giving their advantages and limitations. We also report on indirect mucus assessment procedures (questionnaires, high-resolution computed tomography scanning of the chest, lung function tests). Finally, we consider ways of integrating these techniques with current and future therapeutic options. Cystic fibrosis will not be discussed given its monogenic nature. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
29. Rheology predicts sputum eosinophilia in patients with muco-obstructive lung diseases.
- Author
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Volpato, Mathilde, Vialaret, Jerome, Hirtz, Christophe, Petit, Aurélie, Suehs, Carey, Patarin, Jérémy, Matzner-Lober, Eric, Vachier, Isabelle, Molinari, Nicolas, Bourdin, Arnaud, and Charriot, Jeremy
- Subjects
- *
EOSINOPHILIA , *MUCOCILIARY system , *LUNGS , *LUNG diseases , *NUCLEIC acid amplification techniques , *SPUTUM , *RHEOLOGY - Abstract
Mucus is known to play a pathogenic role in muco-obstructive lung diseases, but little is known about the determinants of mucus rheology. The purpose of this study is to determine which sputum components influence sputum rheology in patients with muco-obstructive lung diseases. We performed a cross sectional prospective cohort study. Spontaneous sputum was collected from consecutive patients with muco-obstructive lung diseases. Sputum rheology was assessed using the Rheomuco® rheometer (Rheonova, Grenoble); the elastic modulus G′, viscous modulus G″, and the critical stress threshold σc were recorded. Key quantitative and qualitative biological sputum components were determined by cytology, nucleic acid amplification tests and mass spectrometry. 48 patients were included from January to August 2019. Among them, 10 had asthma, 14 COPD and 24 non-CF bronchiectasis (NCFB). The critical stress threshold σc predicted a sputum eosinophilia superior to 1.25% with 89.19% accuracy (AUC = 0.8762). G′ and G″ are positively correlated with MUC5AC protein concentration ((rho = 0.361; P =.013) and (rho = 0.335; P =.021), respectively). σc was positively correlated with sputum eosinophilia (rho = 0.394; P =.012), MUC5B (rho = 0.552; P <.001) and total protein (rho = 0.490; P <.001) concentrations. G′ and G″ were significantly higher in asthma patients (G' = 14.49[7.18–25.26]Pa, G'' = 3.0[2.16–5.38]Pa) compared to COPD (G' = 5.01[2.94–6.48]Pa, P =.010; G'' = 1.45[1.16–1.94]Pa, P =.006) and to NCFB (G' = 4.99[1.49–10.49]Pa, P =.003; G" = 1.46[0.71–2.47]Pa, P =.002). In muco-obstructive lung diseases, rheology predicts sputum eosinophilia and is correlated with mucin concentrations, regardless of the underlying disease. (registrar, website, and registration number), where applicable NCT04081740. • Sputum rheology is correlated with mucin content and eosinophils. • Critical stress threshold predicts sputum eosinophilia >1.25% regardless of the underlying disease. • Rheology-based diagnosis and therapeutic strategies can now be tested. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
30. Combining gene expression and genetic analyses to identify candidate genes involved in cold responses in pea.
- Author
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Legrand, Sylvain, Marque, Gilles, Blassiau, Christelle, Bluteau, Aurélie, Canoy, Anne-Sophie, Fontaine, Véronique, Jaminon, Odile, Bahrman, Nasser, Mautord, Julie, Morin, Julie, Petit, Aurélie, Baranger, Alain, Rivière, Nathalie, Wilmer, Jeroen, Delbreil, Bruno, and Lejeune-Hénaut, Isabelle
- Subjects
- *
GENE expression in plants , *PLANT genes , *PEAS , *EFFECT of temperature on plants , *PLANT growth , *POLYMERASE chain reaction - Abstract
Abstract: Cold stress affects plant growth and development. In order to better understand the responses to cold (chilling or freezing tolerance), we used two contrasted pea lines. Following a chilling period, the Champagne line becomes tolerant to frost whereas the Terese line remains sensitive. Four suppression subtractive hybridisation libraries were obtained using mRNAs isolated from pea genotypes Champagne and Terese. Using quantitative polymerase chain reaction (qPCR) performed on 159 genes, 43 and 54 genes were identified as differentially expressed at the initial time point and during the time course study, respectively. Molecular markers were developed from the differentially expressed genes and were genotyped on a population of 164 RILs derived from a cross between Champagne and Terese. We identified 5 candidate genes colocalizing with 3 different frost damage quantitative trait loci (QTL) intervals and a protein quantity locus (PQL) rich region previously reported. This investigation revealed the role of constitutive differences between both genotypes in the cold responses, in particular with genes related to glycine degradation pathway that could confer to Champagne a better frost tolerance. We showed that freezing tolerance involves a decrease of expression of genes related to photosynthesis and the expression of a gene involved in the production of cysteine and methionine that could act as cryoprotectant molecules. Although it remains to be confirmed, this study could also reveal the involvement of the jasmonate pathway in the cold responses, since we observed that two genes related to this pathway were mapped in a frost damage QTL interval and in a PQL rich region interval, respectively. [Copyright &y& Elsevier]
- Published
- 2013
- Full Text
- View/download PDF
31. Strawberry phenotypic plasticity in flowering time is driven by interaction between genetic loci and temperature.
- Author
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Prohaska A, Petit A, Lesemann S, Rey-Serra P, Mazzoni L, Masny A, Sánchez-Sevilla JF, Potier A, Gaston A, Klamkowski K, Rothan C, Mezzetti B, Amaya I, Olbricht K, and Denoyes B
- Abstract
The flowering time (FT), which determines when fruits or seeds can be harvested, is subject to phenotypic plasticity, i.e. the ability of a genotype to display different phenotypes in response to environmental variations. Here, we investigated how the environment affects the genetic architecture of FT in cultivated strawberry (Fragaria ×ananassa) and modifies its QTL effects. To this end, we used a bi-parental segregating population grown for two years at widely divergent latitudes (5 European countries) and combined climatic variables with genomic data (Affymetrix® SNP array). Examination, using different phenological models, of the response of FT to photoperiod, temperature and global radiation, indicated that temperature is the main driver of FT in strawberry. We next characterized in the segregating population the phenotypic plasticity of FT by using three statistical approaches that generated plasticity parameters including reaction norm parameters. We detected 25 FT QTL summarized into 10 unique QTL. Mean values and plasticity parameters QTL were co-localized in three of them, including the major 6D_M QTL whose effect is strongly modulated by temperature. The design and validation of a genetic marker for the 6D_M QTL offers great potential for breeding programs, for example for selecting early-flowering strawberry varieties well adapted to different environmental conditions., (© The Author(s) 2024. Published by Oxford University Press on behalf of the Society for Experimental Biology.)
- Published
- 2024
- Full Text
- View/download PDF
32. Exploration of a European-centered strawberry diversity panel provides markers and candidate genes for the control of fruit quality traits.
- Author
-
Prohaska A, Rey-Serra P, Petit J, Petit A, Perrotte J, Rothan C, and Denoyes B
- Abstract
Fruit quality traits are major breeding targets in cultivated strawberry ( Fragaria × ananassa ). Taking into account the requirements of both growers and consumers when selecting high-quality cultivars is a real challenge. Here, we used a diversity panel enriched with unique European accessions and the 50 K FanaSNP array to highlight the evolution of strawberry diversity over the past 160 years, investigate the molecular basis of 12 major fruit quality traits by genome-wide association studies (GWAS), and provide genetic markers for breeding. Results show that considerable improvements of key breeding targets including fruit weight, firmness, composition, and appearance occurred simultaneously in European and American cultivars. Despite the high genetic diversity of our panel, we observed a drop in nucleotide diversity in certain chromosomal regions, revealing the impact of selection. GWAS identified 71 associations with 11 quality traits and, while validating known associations (firmness, sugar), highlighted the predominance of new quantitative trait locus (QTL), demonstrating the value of using untapped genetic resources. Three of the six selective sweeps detected are related to glossiness or skin resistance, two little-studied traits important for fruit attractiveness and, potentially, postharvest shelf life. Moreover, major QTL for firmness, glossiness, skin resistance, and susceptibility to bruising are found within a low diversity region of chromosome 3D. Stringent search for candidate genes underlying QTL uncovered strong candidates for fruit color, firmness, sugar and acid composition, glossiness, and skin resistance. Overall, our study provides a potential avenue for extending shelf life without compromising flavor and color as well as the genetic markers needed to achieve this goal., Competing Interests: The authors declare that there are no conflicts of interest., (© The Author(s) 2024. Published by Oxford University Press on behalf of Nanjing Agricultural University.)
- Published
- 2024
- Full Text
- View/download PDF
33. QTL analysis for ascorbic acid content in strawberry fruit reveals a complex genetic architecture and association with GDP-L-galactose phosphorylase.
- Author
-
Muñoz P, Castillejo C, Gómez JA, Miranda L, Lesemann S, Olbricht K, Petit A, Chartier P, Haugeneder A, Trinkl J, Mazzoni L, Masny A, Zurawicz E, Ziegler FMR, Usadel B, Schwab W, Denoyes B, Mezzetti B, Osorio S, Sánchez-Sevilla JF, and Amaya I
- Abstract
Strawberry ( Fragaria × ananassa ) fruits are an excellent source of L -ascorbic acid (AsA), a powerful antioxidant for plants and humans. Identifying the genetic components underlying AsA accumulation is crucial for enhancing strawberry nutritional quality. Here, we unravel the genetic architecture of AsA accumulation using an F
1 population derived from parental lines 'Candonga' and 'Senga Sengana', adapted to distinct Southern and Northern European areas. To account for environmental effects, the F1 and parental lines were grown and phenotyped in five locations across Europe (France, Germany, Italy, Poland and Spain). Fruit AsA content displayed normal distribution typical of quantitative traits and ranged five-fold, with significant differences among genotypes and environments. AsA content in each country and the average in all of them was used in combination with 6,974 markers for quantitative trait locus (QTL) analysis. Environmentally stable QTLs for AsA content were detected in linkage group (LG) 3A, LG 5A, LG 5B, LG 6B and LG 7C. Candidate genes were identified within stable QTL intervals and expression analysis in lines with contrasting AsA content suggested that GDP-L-Galactose Phosphorylase FaGGP(3A), and the chloroplast-located AsA transporter gene FaPHT4;4(7C) might be the underlying genetic factors for QTLs on LG 3A and 7C, respectively. We show that recessive alleles of FaGGP(3A) inherited from both parental lines increase fruit AsA content. Furthermore, expression of FaGGP(3A) was two-fold higher in lines with high AsA. Markers here identified represent a useful resource for efficient selection of new strawberry cultivars with increased AsA content., Competing Interests: The authors declare no competing interest., (© The Author(s) 2023. Published by Oxford University Press on behalf of Nanjing Agricultural University.)- Published
- 2023
- Full Text
- View/download PDF
34. CCSP counterbalances airway epithelial-driven neutrophilic chemotaxis.
- Author
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Knabe L, Petit A, Vernisse C, Charriot J, Pugnière M, Henriquet C, Sasorith S, Molinari N, Chanez P, Berthet JP, Suehs C, Vachier I, Ahmed E, and Bourdin A
- Subjects
- Humans, Interleukin-8 metabolism, Interleukin-8 pharmacology, Neutrophils drug effects, Pulmonary Disease, Chronic Obstructive metabolism, Recombinant Proteins pharmacology, Smoking, Sputum cytology, Bronchioles pathology, Chemotaxis, Leukocyte, Neutrophils cytology, Pulmonary Disease, Chronic Obstructive pathology, Uteroglobin pharmacology
- Abstract
Club cell secretory protein (CCSP) knockout mice exhibit increased airway neutrophilia, as found in chronic obstructive pulmonary disease (COPD). We therefore investigated whether treating COPD airway epithelia with recombinant human CCSP (rhCCSP) could dampen exaggerated airway neutrophilia.Control, smoker and COPD air-liquid interface (ALI) cultures exposed to cigarette smoke extract (CSE) were treated with and without rhCCSP. The chemotactic properties of the supernatants were assessed using Dunn chambers. Neutrophil chemotaxis along recombinant human interleukin 8 (rhIL8) gradients (with and without rhCCSP) was also determined. rhCCSP-rhIL8 interactions were tested through co-immunoprecipitation, Biacore surface plasmon resonance (SPR) and in silico modelling. The relationship between CCSP/IL8 concentration ratios in the supernatant of induced sputum from COPD patients versus neutrophilic airway infiltration assessed in lung biopsies was assessed.Increased neutrophilic chemotactic activity of CSE-treated ALI cultures followed IL8 concentrations and returned to normal when supplemented with rhCCSP. rhIL8-induced chemotaxis of neutrophils was reduced by rhCCSP. rhCCSP and rhIL8 co-immunoprecipitated. SPR confirmed this in vitro interaction (equilibrium dissociation constant=8 µM). In silico modelling indicated that this interaction was highly likely. CCSP/IL8 ratios in induced sputum correlated well with the level of small airway neutrophilic infiltration (r
2 =0.746, p<0.001).CCSP is a biologically relevant counter-balancer of neutrophil chemotactic activity. These different approaches used in this study suggest that, among the possible mechanisms involved, CCSP may directly neutralise IL8., Competing Interests: Conflict of interest: L. Knabe has nothing to disclose. Conflict of interest: A. Petit has nothing to disclose. Conflict of interest: C. Vernisse has nothing to disclose. Conflict of interest: J. Charriot has nothing to disclose. Conflict of interest: M. Pugnière has nothing to disclose. Conflict of interest: C. Henriquet has nothing to disclose. Conflict of interest: S. Sasorith has nothing to disclose. Conflict of interest: N. Molinari has nothing to disclose. Conflict of interest: P. Chanez has nothing to disclose. Conflict of interest: J-P. Berthet has nothing to disclose. Conflict of interest: C. Suehs has nothing to disclose. Conflict of interest: I. Vachier has nothing to disclose. Conflict of interest: E. Ahmed has nothing to disclose. Conflict of interest: A. Bourdin has nothing to disclose., (Copyright ©ERS 2019.)- Published
- 2019
- Full Text
- View/download PDF
35. Narrowing down the single homoeologous FaPFRU locus controlling flowering in cultivated octoploid strawberry using a selective mapping strategy.
- Author
-
Perrotte J, Gaston A, Potier A, Petit A, Rothan C, and Denoyes B
- Subjects
- Genetic Linkage genetics, Genome, Plant genetics, Plant Proteins genetics, Plant Proteins metabolism, Polyploidy, Flowers genetics, Fragaria genetics
- Abstract
Extending the period of fruit production is a way to substantially increase crop yield in many fruit or ornamental species. In the cultivated octoploid strawberry (Fragaria × ananassa), the most consumed small fruit worldwide, fruit production season can be extended by selecting the perpetual flowering (PF) cultivars. This trait is of considerable interest to growers and to the food industry. Four homoeologous loci controlling a single trait can be expected in such a complex octoploid species. However, we recently showed that the PF trait is under the control of the single dominant FaPFRU locus (J. Exp. Bot., 2013, 64, 1837), making it potentially amenable to marker-assisted selection (MAS). Here, we report the successful use of a strategy, based on a selective mapping using a reduced sample of individuals, to identify nine markers in close linkage to the FaPFRU allelic variant. Thus, this strategy can be used to fine map the target homoeologous loci in other complex polyploid crop species. Recombinant analysis further enabled us to reduce the locus to a region flanked by two markers, Bx083_206 and Bx215_131, corresponding to a 1.1 Mb region in the diploid F. vesca reference genome. This region comprises 234 genes, including 15 flowering associated genes. Among these, the FLOWERING LOCUS T (FT) is known to be a key activator of flowering. The close association between the PF trait and the FaPFRU flanking markers was validated using an additional segregating population and genetic resources. This study lays the foundation for effective and rapid breeding of PF strawberry cultivars by MAS., (© 2016 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.)
- Published
- 2016
- Full Text
- View/download PDF
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