45 results on '"Sadeghi, Yousef"'
Search Results
2. Bone Marrow Mesenchymal Stem Cell Condition Medium Loaded on PCL Nanofibrous Scaffold Promoted Nerve Regeneration After Sciatic Nerve Transection in Male Rats
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Raoofi, Amir, Sadeghi, Yousef, Piryaei, Abbas, Sajadi, Ensieh, Aliaghaei, Abbas, Rashidiani-Rashidabadi, Ali, Fatabadi, Fatemeh Fadaei, Mahdavi, Behnam, Abdollahifar, Mohammad-Amin, and Khaneghah, Amin Mousavi
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- 2021
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3. Tissue Plasminogen Activator Loaded PCL Nanofibrous Scaffold Promoted Nerve Regeneration After Sciatic Nerve Transection in Male Rats
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Sajadi, Ensieh, Aliaghaei, Abbas, Farahni, Reza Mastery, Rashidiani-Rashidabadi, Ali, Raoofi, Amir, Sadeghi, Yousef, Bagheri, Mohammad, Ilkhani, Saba, and Abdollahifar, Mohammad-Amin
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- 2021
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4. Neuroanatomical changes of the medial prefrontal cortex of male pups of Wistar rat after prenatal and postnatal noise stress
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Teimouri, Mahtab, Heidari, Mohammad Hassan, Amini, Abdollah, Sadeghi, Yousef, Abdollahifar, Mohammad-Amin, Aliaghaei, Abbas, Khavanin, Ali, Nadri, Farshad, Danyali, Samira, and Sanchooli, Tayebeh
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- 2020
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5. Correction to: Subcellular localization of L-selectin ligand in the endometrium implies a novel function for pinopodes in endometrial receptivity
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Nejatbakhsh, Reza, Kabir-Salmani, Maryam, Dimitriadis, Evdokia, Hosseini, Ahmad, Taheripanah, Robabeh, Sadeghi, Yousef, Akimoto, Yoshihiro, and Iwashita, Mitsutoshi
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- 2021
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6. Impairment Induction by Axotomy in Motor Functional and Histological Anterior Horn of Spinal Cord Structure/Induccion del Deterioro por Axotomia en la Estructura Motora Funcional e Histologica del Asta Anterior de la Medula Espinal
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Raoofi, Amir, Gharibian, Saeed, and Sadeghi, Yousef
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- 2019
7. Human Umbilical Cord Matrix Stem Cells Reverse Oxidative Stress-Induced Cell Death and Ameliorate Motor Function and Striatal Atrophy in Rat Model of Huntington Disease
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Ebrahimi, Mohammad Javad, Aliaghaei, Abbas, Boroujeni, Mahdi Eskandarian, Khodagholi, Fariba, Meftahi, Gholamhoussein, Abdollahifar, Mohammad Amin, Ahmadi, Houssein, Danyali, Samira, Daftari, Mahtab, and Sadeghi, Yousef
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- 2018
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8. Resveratrol Protects Purkinje Neurons and Restores Muscle Activity in Rat Model of Cerebellar Ataxia
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Ghorbani, Zeynab, Farahani, Reza Mastery, Aliaghaei, Abbas, Khodagholi, Fariba, Meftahi, Gholam houssein, Danyali, Samira, Abdollahifar, Mohammad Amin, Daftari, Mahtab, Boroujeni, Mahdi Eskandarian, and Sadeghi, Yousef
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- 2018
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9. Sertoli Cells Avert Neuroinflammation-Induced Cell Death and Improve Motor Function and Striatal Atrophy in Rat Model of Huntington Disease
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Ahmadi, Houssein, Boroujeni, Mahdi Eskandarian, Sadeghi, Yousef, Abdollahifar, Mohammad Amin, Khodagholi, Fariba, Meftahi, Gholam Houssein, Hadipour, Mohammadmehdi, Bayat, Amir-Hossein, Shaerzadeh, Fatemeh, and Aliaghaei, Abbas
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- 2018
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10. Osteogenic differentiation of human adipose-derived mesenchymal stem cells on gum tragacanth hydrogel
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Haeri, Seyed Mohammad Jafar, Sadeghi, Yousef, Salehi, Mohammad, Farahani, Reza Masteri, and Mohsen, Nourozian
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- 2016
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11. Long-Term Effects of Hippocampal Low-Frequency Stimulation on Pro-Inflammatory Factors and Astrocytes Activity in Kindled Rats
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Rohani, Razieh, Aliaghaei, Abbas, Abdollahifar, Mohammad-Amin, Sadeghi, Yousef, Zare, Leila, Dehghan, Samaneh, and Heidari, Mohammad Hassan
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Cellular and Molecular Biology ,Epilepsy ,Physiology ,GFAP ,Interleukin-6 ,Deep Brain Stimulation ,TNF-α ,Original Article - Abstract
Objective Epilepsy is accompanied by inflammation, and the anti-inflammatory agents may have anti-seizure effects. In this investigation, the effect of deep brain stimulation, as a potential therapeutic approach in epileptic patients, was investigated on seizure-induced inflammatory factors. Materials and Methods In the present experimental study, rats were kindled by chronic administration of pentylenetetrazol (PTZ; 34 mg/Kg). The animals were divided into intact, sham, low-frequency deep brain stimulation (LFS), kindled, and kindled +LFS groups. In kindled+LFS and LFS groups, animals received four trains of intra-hippocampal low-frequency deep brain stimulation (LFS) at 20 minutes, 6, 24, and 30 hours after the last PTZ injection. Each train of LFS contained 200 pulses at 1 Hz, 200 µA, and 0.1 ms pulse width. One week after the last PTZ injection, the Y-maze test was run, and then the rats’ brains were removed, and hippocampal samples were extracted for molecular assessments. The gene expression of two pro-inflammatory factors [interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α)], and glial fibrillary acidic protein (GFAP) immunoreactivity (as a biological marker of astrocytes reactivation) were evaluated. Results Obtained results showed a significant increase in the expression of of interleukin-6 (IL-6), tumor necrosis factor (TNF)-α, and GFAP at one-week post kindling seizures. The application of LFS had a long-lasting effect and restored all of the measured changes toward normal values. These effects were gone along with the LFS improving the effect on working memory in kindled animals. Conclusion The anti-inflammatory action of LFS may have a role in its long-lasting improving effects on seizure-induced cognitive disorders.
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- 2021
12. Effect of low-frequency stimulation on kindling induced changes in rat dentate gyrus: an ultrastructural study
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Rohani, Razieh, Piryaei, Abbas, Jahanshahi, Ali, Sadeghi, Yousef, and Mirnajafi-Zadeh, Javad
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- 2014
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13. Postmortem Study of Molecular and Histological Changes in the CA1 Hippocampal Region of Chronic Methamphetamine User
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Mahmoudiasl, Gholam-Reza, Abbaszadeh, Hojjat Allah, Rezaei-Tavirani, Mostafa, Abdollahifar, Mohammad-Amin, Sadeghi, Yousef, Khoramgah, Maryam Sadat, Niknazar, Somayeh, and Darabi, Shahram
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nervous system ,GFAP ,Iba1 ,Original Article ,Hippocampous ,Apoptosis ,CA1 ,Methamphetamine - Abstract
Methamphetamine (Meth) is recognized as one of the most important new distributed abused drug that causes severe damage to the different parts of the brain, especially hippocampus. Previous studies have demonstrated that Meth can induce apoptosis and cell death in the brain. In this study, we evaluated the long-term effects of Meth abuse in the CA1 region of postmortem hippocampus. Postmortem molecular and histological analysis was performed for five non-addicted subjects and five Meth addicted ones. Iba-1 (microglia) and glial fibrillary acidic protein, GFAP (astrocytes) expression were assayed by western blotting and immunohistochemistry (IHC) methods. Histopathological assessment was done with stereological counts of hippocampal cells stained with hematoxylin and eosin (H and E). Tunel staining was used to detect DNA damage in human brains. In addition, protein-protein interaction analysis network was investigated. Western blotting and immunohistochemistry assay showed overexpression of GFAP and Iba-1 protein in the CA1 hippocampal region of Meth users' brain. Stereological analysis in the CA1 region revealed increased neuron degeneration. Furthermore, significant apoptosis and cell death were confirmed by Tunel assay in the hippocampus. The prominent role of TLR4, IL1B, CASP1, and NLRP3 in the molecular mechanism of Meth was highlighted via PPI network analysis. Chronic Meth use can induce GFAP and Iba-1 upregulation and neuronal apoptosis in the CA1 region of the postmortem hippocampus.
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- 2019
14. Impairment Induction by Axotomy in Motor Functional and Histological Anterior Horn of Spinal Cord Structure
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Raoofi, Amir, Gharibian, Saeed, and Sadeghi, Yousef
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Anterior horn of spinal cord ,Stereology ,Estereología ,Degenerative ,Motor-funcional ,Axotomy ,Asta anterior ,Motor functional ,Degenerativa ,Axotomía - Abstract
SUMMARY: We have surveyed the motor changes in rats subjected to sciatic nerve axotomy. The rats were divided into two groups, each one consisting of ten animals, which underwent the following intervention: The first group (control): healthy rats without any injuries and experimental group: rats with injured sciatic nerve without treatment. at 12 weeks, the L4 and L5 spinal cord segments were removed. We evaluated nerve function using muscle electromyography (EMG) activity and sciatic function index (SFI) simultaneously with histological spinal cord analyses by stereological methods at 12 week. After nerve injury presented gross locomotor deficits at week 12. We also found that the volume of the anterior horn of spinal cord and total number of motor neurons were decreased after nerve axotomy (p
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- 2019
15. Learning impairment caused by intra-CA1 microinjection of testosterone increases the number of astrocytes
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Emamian, Shahla, Naghdi, Nasser, Sepehri, Houri, Jahanshahi, Mehrdad, Sadeghi, Yousef, and Choopani, Samira
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- 2010
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16. Disorders of sex development and female reproductive capacity: A literature review.
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Hosseinirad, Hossein, Yadegari, Pouya, Mohanazadeh Falahieh, Fatemeh, Nouraei, Soheila, Paktinat, Shahrokh, Afsharzadeh, Nousha, and Sadeghi, Yousef
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SEX differentiation disorders ,REPRODUCTIVE technology ,GONADAL dysgenesis ,ADRENOGENITAL syndrome ,ANDROGEN-insensitivity syndrome ,GENITALIA - Abstract
Disorders of sex development (DSD) are a wide-ranging group of complex conditions that influence chromosomal, gonadal, and phenotypic sex. The prevalence of DSD is very low, but affected patients deserve individualized management to improve psychological, sexual, and reproductive outcomes. This review aims to clarify the fertility potential of DSD patients who can be reared as females and their chance of becoming pregnant, especially using assisted reproductive techniques (ART). Due to the effects of DSD on internal and external genital organs, these conditions result in varying degrees of fertility potential. Fertility rate depends on the phenotype and is inversely related to the severity of the disorder. Reproductive endocrinologists and infertility specialists must be considered active partners of the interdisciplinary treatment team. With current advances in ART, pregnancy is more achievable in patients who were considered infertile at first glance. Due to the complexity of the medical management in DSD patients, more studies should be conducted to conclusively suggest the best choice for improving their fertility potential. Abbreviations: AIS: Androgen Insensitivity Syndrome; AMH: Anti-Müllerian Hormone; ART: Assisted Reproductive Technology; ASRM: American Society for Reproductive Medicine; CAH: Congenital Adrenal Hyperplasia; CAIS: Complete Androgen Insensitivity Syndrome; DHT: Dihydrotestosterone; DSD: Disorders of Sexual Development; FSH: Follicle Stimulating Hormone; GD: Gonadal Dysgenesis; ICSI: Intracytoplasmic Sperm Injection; IUGR: Intrauterine Growth Restriction; IVF: In Vitro Fertilization; IVF-ET: IVF and Embryo Transfer; LH: Luteinizing Hormone; MGD: Mixed Gonadal Dysgenesis; MRI: Magnetic Resonance Imaging; MRKH: Mayer-Rokitansky-Kuster-Hauser; US: Ultrasonography; HSG: Hysterosalpingography; PAIS: Partial Androgen Insensitivity Syndrome; PGD: Preimplantation Genetic Diagnosis; POR: P450 Oxidoreductase; PROM: Premature Rupture of Membranes; TS: Turner Syndrome; 17β-HSD III: 17β-Hydroxysteroid Dehydrogenase III; 21-OHD: 21-hydroxylase deficiency; 5α-RD-2: 5α-reductase-2 [ABSTRACT FROM AUTHOR]
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- 2021
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17. Subcellular localization of L-selectin ligand in the endometrium implies a novel function for pinopodes in endometrial receptivity
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Nejatbakhsh, Reza, Kabir-Salmani, Maryam, Dimitriadis, Eva, Hosseini, Ahmad, Taheripanah, Robabeh, Sadeghi, Yousef, Akimoto, Yoshihiro, and Iwashita, Mitsutoshi
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- 2012
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18. Functional dopaminergic neurons derived from human chorionic mesenchymal stem cells ameliorate striatal atrophy and improve behavioral deficits in Parkinsonian rat model.
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Ebrahimi, Vahid, Eskandarian Boroujeni, Mahdi, Aliaghaei, Abbas, Abdollahifar, Mohammad Amin, Piryaei, Abbas, Haghir, Hossein, and Sadeghi, Yousef
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- 2020
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19. Alterations of neuroimmune cell density and pro-inflammatory cytokines in response to thimerosal in prefrontal lobe of male rats.
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Afsordeh, Kobra, Sadeghi, Yousef, Amini, Abdollah, Namvarpour, Zahra, Abdollahifar, Mohammad-Amin, Abbaszadeh, Hojjat-Allah, and Aliaghaei, Abass
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THIMEROSAL , *CYTOKINES , *HEAVY metal toxicology , *MICROGLIA , *MAST cells - Abstract
Evidence suggests that the effect of heavy metals on neuroimmune cells lead to neurogenic inflammatory responses. In this study, immune cells [mast cells (MCs) and microglia] and pro-neuroinflammation cytokines (interleukin-1b and tumor necrosis factor-α) were assessed in the prefrontal lobe of rat brains exposed to thimerosal in different timeframes. A total of 108 neonatal Wistar rats were divided into three groups having three subgroups. The experimental groups received a single dose of thimerosal (300 μg/kg) postnatally at 7, 9, 11, and 15 days. The vehicle groups received similar injections of phosphate-buffered saline in a similar manner. The control groups received nothing. Samples of the prefrontal cortex were collected and prepared for stereological, immunohistochemical, and molecular studies at timeframes of 12 or 48 h (acute phase) and 8 days (subchronic phase) after the last injection. The average density of the microglia and MCs increased significantly in the experimental groups. This increase was more evident in the 48 h group. At 8 days after the last injection, there was a significant decrease in the density of the MCs compared to the 12 and 48 h groups. Alterations in pro-inflammatory cytokines were significant for all timeframes. This increase was more evident in the 48 h group after the last injection. There was a significant decrease in both neuroinflammatory cytokines at 8 days after the last injection. It was found that ethylmercury caused abnormal neurogenic inflammatory reactions and alterations in the neuroimmune cells that remained for a longer period in the brain than in the blood. [ABSTRACT FROM AUTHOR]
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- 2019
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20. Peripheral axotomy-induced changes of motor function and histological structure of spinal anterior horn.
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Raoofi, Amir, Abdollahifar, Mohammad Amin, Aliaghaei, Abbas, Piryaei, Abbas, Hejazi, Fatemeh, Sajadi, Ensieh, Rashidiani-Rashidabadi, Ali, and Sadeghi, Yousef
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LEG muscles ,ASTROCYTES ,SCIATIC nerve ,PERIPHERAL nervous system ,RAT control ,SPINAL cord ,EXPERIMENTAL groups - Abstract
The aim of this study was to evaluate changes of both peripheral motor function and histology of spinal anterior horn in adult rats after unilateral sciatectomy. Ten adult healthy rats served as control group, while in the ten rat experimental group the right sciatic nerve was severed. We followed-up nerve motor function using a sciatic function index and electromyography activity of the gastrocnemious muscle. The rats of the experimental group presented the expected gross locomotor deficit and leg muscle atrophy. At 12 weeks post sciatectomy, L4 and L5 spinal cord segments were removed from the twenty rats and were analysed by istological stereological methods. In the axotomized animals volume of the anterior horn and its content of motor neurons decreased, while the content of astrocytes increased (p < 0.05). Thus, in adult rats, beside the obvious peripheral nerve disfuction, the sciatic nerve axotomy have severe consequences on the soma of the injured motor neurons in the spinal anterior horn. All these quantitative analyses may be usefull to quantify changes occurring in adult animals after axotomy and eventual management to modify the final outcomes in peripheral nerve disorders. [ABSTRACT FROM AUTHOR]
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- 2019
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21. Stem cell transplantation and functional recovery after spinal cord injury: a systematic review and meta-analysis.
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Abbaszadeh, Hojjat-Allah, Niknazar, Somayeh, Darabi, Shahram, Roozbahany, Navid Ahmady, Noori-Zadeh, Ali, Ghoreishi, Seyed Kamran, Khoramgah, Maryam Sadat, and Sadeghi, Yousef
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STEM cell transplantation ,SPINAL cord injuries ,BRUISES - Abstract
Spinal cord injury is a significant cause of motor dysfunctions. There is no definite cure for it, and most of the therapeutic modalities are only symptomatic treatment. In this systematic review and meta-analysis, the effectiveness of stem cell therapy in the treatment of the spinal cord injuries in animal models was studied and evaluated. A systematic search through medical databases by using appropriate keywords was conducted. The relevant reports were reviewed in order to find out cases in which inclusion and exclusion criteria had been fulfilled. Finally, 89 articles have been considered, from which 28 had sufficient data for performing statistical analyses. The findings showed a significant improvement in motor functions after cell therapy. The outcome was strongly related to the number of transplanted cells, site of injury, chronicity of the injury, type of the damage, and the induction of immune-suppression. According to our data, improvements in functional recovery after stem cell therapy in the treatment of spinal cord injury in animal models was noticeable, but its outcome is strongly related to the site of injury, number of transplanted cells, and type of transplanted cells. [ABSTRACT FROM AUTHOR]
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- 2018
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22. Mesenchymal Stem Cells as an Alternative for Schwann Cells in Rat Spinal Cord Injury
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Zaminy, Arash, Shokrgozar, Mohammad Ali, Sadeghi, Yousef, Noroozian, Mohsen, Heidari, Mohammad Hassan, and Piryaei, Abbas
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Male ,Tissue Scaffolds ,Basic Sciences ,Bone Marrow Cells ,Mesenchymal Stem Cells ,Recovery of Function ,Flow Cytometry ,Mesenchymal Stem Cell Transplantation ,Axons ,Nerve Regeneration ,Rats ,Gene Expression Regulation ,Spinal Cord ,Cell Transdifferentiation ,Animals ,Original Article ,Cell Lineage ,RNA, Messenger ,Schwann Cells ,Rats, Wistar ,Myelin Sheath ,Spinal Cord Injuries - Abstract
Background: Spinal cord has a limited capacity to repair; therefore, medical interventions are necessary for treatment of injuries. Transplantation of Schwann cells has shown a great promising result for spinal cord injury (SCI). However, harvesting Schwann cell has been limited due to donor morbidity and limited expansion capacity. Furthermore, accessible sources such as bone marrow stem cells have drawn attentions to themselves. Therefore, this study was designed to evaluate the effect of bone marrow-derived Schwann cell on functional recovery in adult rats after injury. Methods: Mesenchymal stem cells were cultured from adult rats’ bone marrow and induced into Schwann cells in vitro. Differentiation was confirmed by immunocytochemistry and RT-PCR. Next, Schwann cells were seeded into collagen scaffolds and engrafted in 3 mm lateral hemisection defects. For 8 weeks, motor and sensory improvements were assessed by open field locomotor scale, narrow beam, and tail flick tests. Afterwards, lesioned spinal cord was evaluated by conventional histology and immunohistochemistry. Results: In vitro observations showed that differentiated cells had Schwann cell morphology and markers. In this study, we had four groups (n = 10 each): laminectomy, control, scaffold and scaffold + Schwann cells. Locomotor and sensory scores of cell grafted group were significantly better than control and scaffold groups. In histology, axonal regeneration and remyelination were better than control and scaffold groups. Conclusion: This study demonstrates that bone marrow-derived Schwann cells can be considered as a cell source for Schwann cells in SCI treatment.
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- 2013
23. Decrease in Cavity Size and Oligodendrocyte Cell Death Using Neurosphere-Derived Oligodendrocyte-Like Cells in Spinal Cord Contusion Model.
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Abbaszadeh, Hojjat Allah, Tiraihi, Taki, Sadeghi, Yousef, Delshad, Ali Reza, Sadeghizadeh, Majid, Taheri, Taher, and Noori-Zadeh, Ali
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- 2018
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24. Evaluation of the spatial arrangement of Purkinje cells in ataxic rat's cerebellum after Sertoli cells transplantation.
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Mohammadi, R., Heidari, M. H., Sadeghi, Y., Abdollahifar, M.-A., Aghaei, A., Mohammadi, Rabieh, Heidari, Mohammad Hassan, Sadeghi, Yousef, Abdollahifar, Mohammad Amin, and Aghaei, Abbas
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Background: Purkinje cells (PCs) pathology is important in cerebellar disorders like ataxia. The spatial arrangement of PCs after different treatments has not been studied extensively. Immunohistochemistry (IHC) analysis of cerebellum can give a proper tool for explaining the pathophysiology of PCs in ataxia. Here we stereologically analysed the 3-dimensional spatial arrangement of PCs in the cerebellum of rats after ataxia induction with 3-acetylpyridine (3-AP).Materials and Methods: Ataxia was induced in rats by intraperitoneal injection of 3-AP (65 mg/kg). Spatial arrangement of PCs for differences in ataxic rats with (3-AP-SC) or without (3-AP) Sertoli cells (SCs) transplantation was evaluated using second-order stereology. The IHC method by using antibodies to anti-calbindin in the cerebellum was applied.Results: Our results showed that a random arrangement is at larger distances between PCs in 3-AP and 3-Ap-SC groups. Therefore the PCs are not normally arranged after 3-AP and SCs transplantation stored the spatial arrangements of the cells after ataxia induction in rats. IHC analyse shows that number of PCs was significantly improved after the SC transplantation.Conclusions: Segregation of PCs can be observed at some areas in the ataxic rats' cerebellum. However, the spatial arrangement of PCs was unchanged in SCs transplanted rats. (Folia Morphol 2018; 77, 2: 194-200). [ABSTRACT FROM AUTHOR]- Published
- 2018
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25. The Neuroprotective Effects of Flaxseed Oil Supplementation on Functional Motor Recovery in a Model of Ischemic Brain Stroke: Upregulation of BDNF and GDNF.
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Bagheri, Abolqasem, Talei, Sahand, Hassanzadeh, Negar, Mokhtari, Tahmineh, Akbari, Mohammad, Malek, Fatemeh, Jameie, Seyed Behnamedin, Sadeghi, Yousef, and Hassanzadeh, Gholamreza
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STROKE ,LINSEED oil ,NEUROPROTECTIVE agents ,MOTOR neurons ,BRAIN-derived neurotrophic factor ,GLIAL cell line-derived neurotrophic factor ,DIETARY supplements ,GENETICS ,THERAPEUTICS - Abstract
Cerebral ischemic stroke is a common leading cause of disability. Flaxseed is a richest plant-based source of antioxidants. In this study, the effects of flaxseed oil (FSO) pretreatment on functional motor recovery and gene expression and protein content of neurotrophic factors in motor cortex area in rat model of brain ischemia/reperfusion (I/R) were assessed. Transient middle cerebral artery occlusion (tMCAo) in rats was used as model brain I/R. Rats (6 in each group) were randomly divided into four groups of Control (Co+normal saline [NS]), Sham (Sh+NS), tMCAo+NS and tMCAo+FSO. After three weeks of pretreatment with vehicle or FSO (0.2 ml~800 mg/kg body weight), the rats were operated in sham and ischemic groups. Ischemia was induced for 1 h and then reperfused. After 24 h of reperfusion, neurological examination was performed, and animals were sacrificed, and their brains were used for molecular and histopathological studies. FSO significantly improved the functional motor recovery compared with tMCAo+NS group (P<0.05). A significant reduction in brain-derived neurotrophic factor (BDNF) and glial cell-derived neurotrophic factor (GDNF) mRNAs and protein levels were observed in the tMCAo+NS group compared with Co+NS and Sh+NS group (P<0.05). A significant increase of BDNF and GDNF mRNAs and proteins was recorded in the tMCAo+FSO group compared with Co+NS, Sh+NS and tMCAO+NS groups (P<0.05). The results of the current study demonstrated that pretreatment with FSO had neuroprotective effects on motor cortex area following cerebral ischemic stroke by increasing the neurotrophic factors (BDNF, GDNF). [ABSTRACT FROM AUTHOR]
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- 2017
26. Er:YAG Laser and Cyclosporin A Effect on Cell Cycle Regulation of Human Gingival Fibroblast Cells.
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Abbaszadeh, Hojjat-Allah, Peyvandi, Ali Asghar, Sadeghi, Yousef, Safaei, Akram, Zamanian-Azodi, Mona, Khoramgah, Maryam Sadat, and Rezaei-Tavirani, Mostafa
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INFLAMMATORY bowel diseases ,BONE resorption ,GENE ontology - Abstract
Introduction: Periodontitis is a set of inflammatory disorders characterized by periodontal attachment loss and alveolar bone resorption. Because of deficiency in periodontitis mechanical therapy, this study was aimed to explore the molecular influence of the erbiumdoped: yttrium aluminum garnet (Er:YAG) laser and cyclosporin A (CsA) on human gingival fibroblasts (HGFs) for improvement in periodontal diseases therapy. Methods: We focused on articles that studied the proteome profiles of HGFs after treatment with laser irradiation and application of CsA. The topological features of differentially expressed proteins were analyzed using Cytoscape Version 3.4.0 followed by module selection from the protein-protein interaction (PPI) network using Cluster ONE plugin. In addition, we performed gene ontology (GO) enrichment analysis for the densely connected region and key proteins in both PPI networks. Results: Analysis of PPI network of Er:YAG laser irradiation on HGFs lead to introducing YWHAZ, VCP, HNRNPU, YWHAE, UBA52, CLTC, FUS and IGHG1 as key proteins while similar analysis revealed that ACAT1, CTSD, ALDOA, ANXA2, PRDX1, LGALS3, ARHGDI and EEF1A1 are the crucial proteins related to the effect of drug. GO enrichment analysis of hubbottleneck proteins of the 2 networks showed the different significant biological processes and cellular components. The functional enrichments of module of Er:YAG laser network are included as fatty acid transmembrane transport, cytokinesis, regulation of RNA splicing and asymmetric protein localization. There are not any significant clusters in network of HGF treated by CsA. Conclusion: The results indicate that there are 2 separate biomarker panels for the 2 treatment methods. [ABSTRACT FROM AUTHOR]
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- 2017
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27. On the square subgroups of decomposable torsion-free abelian groups of rank three.
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Hasani, Fysal, Karimi, Fatemeh, Najafizadeh, Alireza, and Sadeghi, Yousef
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- 2016
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28. Astrocytes in Molecular Layer of Cerebellum after Spatial Learning.
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Jahanshahi, Mehrdad, Sadeghi, Yousef, Hosseini, Ahmad, Naghdi, Naser, and Golalipour, Mohammad Jafar
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CEREBELLUM , *MOTOR learning , *CEREBELLAR cortex , *ASTROCYTES , *SHORT-term memory - Abstract
Introduction: Previous studies have suggested that the cerebellum is a primary site of motor learning. The cerebellar cortex has a particular glial architecture with large astroglial cells. In addition, more recent works have revealed that astrocytes play a more active role in neuronal activity. The aim of this study was to evaluate the number of astrocytes in the molecular layer of rat's cerebellum after spatial learning. Methods: 21 male albino wistar rats were used in this study. Reference and working memory methods of Morris Water Maze (MWM) were used. Following behavioral testing, animals were decapitated under diethyl ether anesthesia. Brains were removed and fixed for 2 weeks for histological assessment. Finally, 7 µm thick coronal slices were cut and stained with PTAH staining for showing the astrocytes. Results: Our results showed a significant difference in the number of astrocytes between the control, reference and working memory groups. On the other hand, the number of astrocytes in the working memory group was more than the other groups. There was no difference in density of astrocytes between the lateral and medial parts of the cerebellum in any group. It seems that the distribution of astrocytes in the lateral and medial parts of cerebellum is similar. Discussion: We concluded that spatial learning such as reference or working memory methods, can increase the number of astrocytes in the cerebellum and this increase is similar in the cerebellar cortex. [ABSTRACT FROM AUTHOR]
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- 2012
29. The evaluation of cyclic uniaxial strain on myogenic differentiation of adipose-derived stem cells.
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Bayati, Vahid, Sadeghi, Yousef, Shokrgozar, Mohammad Ali, Haghighipour, Nooshin, Azadmanesh, Kayhan, Amanzadeh, Amir, and Azari, Shahram
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MYOBLASTS ,CELL differentiation ,ADIPOSE tissues ,STEM cells ,MUSCLE cells ,CELLULAR signal transduction ,HOMEOSTASIS ,REGENERATION (Biology) - Abstract
Abstract: It has been revealed that skeletal muscle cells have the potential to generate, sense and respond to biomechanical signals and that, mechanical force is one of the important factors influencing proliferation, differentiation, regeneration and homeostasis of skeletal muscle cells and myoblasts. The aim of this study was to illustrate the effect of cyclic uniaxial strain on myogenic differentiation of adipose-derived stem cells (ASCs). This study was designed to investigate this effect within 3 days in 4 groups: control (untreated), chemical, chemical-mechanical and mechanical based on exposure of ASCs to chemical growth factors for 3 days or to mechanical strain just on the 2nd day. Finally, cell orientation, muscle-related gene expression, myosin protein synthesis and the number of myosin-positive cells were examined to estimate the rate of differentiation. By studying the cells before and after exposure to uniaxial strain, it could be observed that by exerting the load, the cells were organized almost perpendicularly to strain direction. Real-time RT-PCR demonstrated that uniaxial strain had a significant effect on up-regulation of muscle-related genes in chemical–mechanical group (P <0.001) as compared to mechanical or chemical groups. Immunocytochemistry confirmed the myosin-positive cells in treated groups and the numbers of these cells were enumerated by flow cytometry. These data suggest that uniaxial cyclic strain could affect ASCs and cause their myogenic differentiation and that the combination of chemical myogenic differentiation factors with mechanical signals promotes differentiation much more than differentiation by chemical myogenic differentiation factors or mechanical signals alone. [Copyright &y& Elsevier]
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- 2011
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30. Transplantation of Schwann Cells Differentiated from Adipose Stem Cells Improves Functional Recovery in Rat Spinal Cord Injury.
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Zaminy, Arash, Shokrgozar, Mohammad Ali, Sadeghi, Yousef, Norouzian, Mohsen, Heidari, Mohammad Hassan, and Piryaei, Abbas
- Abstract
Background: When the spinal cord is damaged, medical procedures are vital to prevent of improvement of the lesion. Because of poor regeneration ability of central nervous tissue, the most injuries are irreversible. One of encouraging interventions for treatment of spinal cord injury is Schwann cell transplantation. However, isolation of Schwann cell for clinical interventions is complicated approach with low cells yield and purity. Thus, easily accessed sources like Adipose mesenchymal stem cells have been taken notice. Therefore, this study was planned to assess the effect of adipose stromal cell-derived Schwann cell transplantation in functional recovery after lateral hemisection in adult rats. Methods: After isolation, adipose stem cells were differentiated to Schwann cells. The differentiation was verified by immunocytochemistry and reverse transcription-polymerase chain reaction (RT-PCR). Then, we loaded the cells into collagen scaffolds with parallel aligned canals and transplanted into rats with 3 mm lesions at T9-T10 level. Motor and sensory improvement were evaluated by open field locomotor scale, narrow beam, and tail flick tests for 60 days. Subsequently, conventional histology and immunohistochemistry were performed. Results: In vitro results revealed that mesenchymal stem cells after differentiation gained Schwann cells morphology and markers. Schwann cell-grafted group had significantly higher locomotor and sensory scores in comparison with the control and scaffold without cell groups. Histological observations showed differentiated cells have the ability to improve axonal regeneration and remyelination. Conclusion: Our study proved that adipose tissue-derived Schwann cells can change the rough environment of damaged spinal cord and support axon regeneration and enhance functional recovery, and possibly be helpful for people suffering from spinal cord injuries. [ABSTRACT FROM AUTHOR]
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- 2013
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31. Transplantation of human dental pulp stem cells compensates for striatal atrophy and modulates neuro-inflammation in 3-nitropropionic acid rat model of Huntington's disease.
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Eskandari, Neda, Boroujeni, Mahdi Eskandarian, Abdollahifar, Mohammad Amin, Piryaei, Abbas, Khodagholi, Fariba, Mirbehbahani, Seyed Hamidreza, Siroosi, Shokoofeh, Moghaddam, Meysam Hassani, Aliaghaei, Abbas, and Sadeghi, Yousef
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HUNTINGTON disease , *ANIMAL disease models , *DENTAL pulp , *STEM cells , *MOTOR ability - Abstract
[Display omitted] • Transplantation of DPSCs improved motor skills and muscle activity in rat brain lesion. • Transplantation of DPSCs prevented the atrophy of the striatum. • Transplantation of DPSCs impeded gliosis and microgliosis in rats. • Transplantation of DPSC downregulated Caspase-3 in brain lesion. • Transplantation of DPSCs decreased the expression of inflammatory cytokines, TNF and IL-1β, at mRNA level. Stem cell-based therapy has recently offered a promising alternative for the remedy of neurodegenerative disorders like Huntington's disease (HD). Herein, we investigated the potential ameliorative effects of implantation of dental pulp stem cells (DPSCs) in 3-nitropropionic acid (3-NP) rat models of HD. In this regard, human DPSCs were isolated, culture-expanded and implanted in rats lesioned with 3-NP. Post-transplantation examinations revealed that DPSCs were able to survive and augment motor skills and muscle activity. Histological analysis showed DPSCs treatment hampered the shrinkage of the striatum along with the inhibition of gliosis and microgliosis in the striatum of 3-NP rat models. We also detected the downregulation of Caspase-3 and pro-inflammatory cytokines such as TNF and IL-1β upon DPSCs grafting. Overall, these findings imply that the grafting of DPSCs could repair motor-skill impairment and induce neurogenesis, probably through the secretion of neurotrophic factors and the modulation of neuroinflammatory response in HD animal models. [ABSTRACT FROM AUTHOR]
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- 2021
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32. Neuro-restorative effect of sertoli cell transplants in a rat model of amyloid beta toxicity.
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Aliaghaei, Abbas, Meymand, Arman Zeinaddini, Boroujeni, Mahdi Eskandarian, Khodagoli, Fariba, Meftahi, Gholam Houssein, Hadipour, Mohammad Mehdi, Abdollahifar, Mohammad Amin, Mesgar, Somaye, Ahmadi, Houssein, Danyali, Samira, Hasani, Sanaz, and Sadeghi, Yousef
- Subjects
- *
SERTOLI cells , *LONG-term synaptic depression , *RATS , *TRANSPLANTATION of organs, tissues, etc. , *ALZHEIMER'S disease , *NEUROPLASTICITY - Abstract
Highlights • Grafted Sertoli cells (SCs) exhibited a decline in apoptosis in a rat model of amyloid beta toxicity. • Implantation of SCs in rats with Aβ1-42 receiving vehicle decreased the amount of gliosis. • We found that implanted SCs can recover hippocampus dependent memory and learning. • Transplanted SCs restored long-term synaptic plasticity in lesioned rats. Abstract Alzheimer's disease (AD) is a degenerative nerve disease which adversely affects memory and learning skills. Currently, there is no disease-modifying therapeutic approach for AD. However, a growing body of literature suggests cell based therapies as a promising remedy for neurological disorders. Among the potential cell sources, testis- derived Sertoli cells (SCs) appear to be an attractive choice due to their immune-privileged capacities. Herein, we investigated the neuro-restorative/protective effects of SC transplants in a rat model of amyloid beta toxicity. To this end, GATA-4 and vimentin positive SCs were transplanted into rats with amyloid beta induced hippocampal lesions. According to our in vivo results, implanted SCs survived, exhibited reduction in both apoptosis as well as astrocytic migration. Additionally, transplantation of SCs restored hippocampus dependent memory and learning, along with the recovery of long-term synaptic plasticity. Taken together, these data indicate that SCs are a valuable source for cell-based therapies particularly aimed at AD. [ABSTRACT FROM AUTHOR]
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- 2019
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33. Cardiac extracellular matrix hydrogel together with or without inducer cocktail improves human adipose tissue-derived stem cells differentiation into cardiomyocyte–like cells.
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Baghalishahi, Masoumeh, Efthekhar-vaghefi, Seyed hasan, Piryaei, Abbas, Nematolahi-mahani, S.N., Mollaei, Hamid Reza, and Sadeghi, Yousef
- Subjects
- *
EXTRACELLULAR matrix , *HYDROGELS , *ADIPOSE tissues , *STEM cells , *CELL differentiation - Abstract
Studies have demonstrated that differentiation of stem cells into cardiomyocytes is a complex phenomenon that requires sufficient inducing factors at various time points. Cardiac extracellular matrix (cECM) could provide tissue specific microenvironment and act as an inductive template for efficient cell differentiation. The aim of this study was to investigate the effect of cECM on differentiation of human adipose tissue-derived stem cells (hADSCs) into cardiomyocytes using cECM hydrogel in combination with a cardiac inductive cocktail. hADSCs were cultured on ECM-coated plates with and without inductive cocktail for 3weeks. qRT-PCR and western blot analysis were used to evaluate the expression pattern of special cardiac genes and proteins. When hADSCs were cultured in the presence of cECM cardiac genes including GATA4, HAND1, HAND2, NKX2.5, Troponin I, βMHC, Connexin43 were highly expressed in differentiated cells. Also Connexin43, cTnI and βMHC proteins were expressed as well. We could show that cECM by itself could affect viability, proliferation and differentiation of hADSCs. However, combination of cECM with a cardiac inducing cocktail could improve the results. [ABSTRACT FROM AUTHOR]
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- 2018
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34. The role of Tetrahydrocannabinol in inducing disrupted signaling cascades, hippocampal atrophy and memory defects.
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Barfi, Elahe, Tehrani, Ava Modirzadeh, Mohammadpanah, Mojtaba, Boroujeni, Mahdi Eskandarian, Meftahi, Gholam Hossein, Sadeghi, Yousef, Eziy, Samira, Khatmi, Aysan, Abdollahifar, Mohammad Amin, Ghorbani, Zeynab, and Aliaghaei, Abbas
- Subjects
- *
LONG-term memory , *TETRAHYDROCANNABINOL , *ATROPHY , *HIPPOCAMPUS (Brain) , *CELL communication - Abstract
• THC administration interrupts long term memory and long term potentiation. • Stereological studies revealed considerable atrophy in hippocampus. • Several signaling cascades related to synaptic regulation were interrupted in hippocampus under THC administration. Tetrahydrocannabinol (THC), a major psychoactive constituent of marijuana, can substantially change the function of several brain areas, leading to behavioral impairment including memory and learning dysfunction. Given the importance of hippocampus as one of the chief parts of the brain involved in memory processing, the present study seeks to investigate structural and histological alterations in hippocampus as well as behavioral defects provoked by THC treatment. Besides, using genome-wide sequencing, we adopted a pathway-based approach to discover dysregulated molecular pathways. Our results demonstrated remarkable hippocampal atrophy, and also interrupted memory function and long term potentiation (LTP) under THC exposure. We also detected several dysregulated signaling pathways involved in synaptic plasticity as well as cell-cell interaction in the hippocampus of THC-treated rats. Overall, the results indicate a potential correlation between disrupted signaling cascades, hippocampal atrophy and memory defects caused by THC treatment. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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35. Alpha lipoic acid ameliorates THIM-induced prefrontal cell loss and abnormal enzymatically contents in the developing rat.
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Noshadian, Mehrazin, Namvarpour, Zahra, Amini, Abdollah, Raoofi, Amir, Atabati, Hadi, Sadeghi, Yousef, Aliaghaei, Abass, and Abdollahifar, Modammad-Amin
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- *
LIPOIC acid , *PREFRONTAL cortex , *VOXEL-based morphometry , *RATS , *DRUGS , *CELLS - Abstract
•.Alpha Lipoic Acid has a protective effect on brain cells. • THIM has a toxic effect on the developing brain. • Alpha Lipoic Acid decreaces the glutathione content of prefrontal cortex. • ALA is one of the important antioxidants that can prevent the adverse effects of THIM-containing products. Thimerosal (THIM) is a common preservative used in many pharmaceutical drugs, vaccines, cosmetics and many other products. Today, it was somewhat clear that Thimerosal (THIM) is a neurotoxicant preservative. We aimed to use of a suitable agent for preventing of THIM side effects on brain. Therefore, in this research, the protective effects of Alpha Lipoic Acid (ALA), against THIM-induced brain cell loss, changes in neuroimmune cell and enzymatically contents were examined. Male Wistar rats (n = 60) were randomly distributed into five groups: 1- THIM group; this group received THIM at dose of 300 μg /kg on 7, 9, 11, 15 days after birth 2- ALA group; received ALA (20 mg/kg) in the same order. 3- THIM & ALA group; this group received ALA in the same dose, 30 min before THIM administration.4& 5; Saline and ALA vehicle groups were also included. At 56th postnatal day, samples of the prefrontal cortex were collected and prepared for stereological, immune-histochemical, and enzymatic evaluations. The result showed that ALA, prevents the adverse effects of THIM on brain cell loss, abnormal changes in neuroimmune cells (p < 0.05), prefrontal cortex volume (p < 0.05), and the glutathione content of prefrontal cortex (p < 0.05). In conclusion, neonatal exposure to THIM can induce abnormal alterations in neuroimmune cells and brain cell density as well as prefrontal cortex volume & glutathione content, and ALA can ameliorate these abnormalities. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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36. Neuroprotective effect of exogenous melatonin on the noradrenergic neurons of adult male rats' locus coeruleus nucleus following REM sleep deprivation.
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Jameie, Seyed Behnamedin, Mesgar, Somaye, Aliaghaei, Abbas, Raoofi, Amir, Amini, Maryam, Khodagholi, Fariba, Danyali, Samira, Sorraya, Marziyeh, and Sadeghi, Yousef
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- *
LOCUS coeruleus , *NORADRENERGIC neurons , *RAPID eye movement sleep , *SLEEP deprivation , *MELATONIN , *TYROSINE hydroxylase , *PINEAL gland - Abstract
Melatonin is primarily secreted by the pineal gland in dark. In addition to its role as an internal sleep facilitator, melatonin acts as an antioxidant, anti-inflammatory and neuroprotective agents. melatonin has been introduced as a therapeutic strategy for sleep disorders. Hence, in the present study, we studied the neuroprotective effects of pre- and post-treatment of melatonin in locus coeruleus nucleus (LC) of rapid eye movement (REM) sleep deprived (REM-SD) male adult rats. Adult male rats of control, sham and trial groups were used Exogenous melatonin (ExMe) was intraperitoneally injected in two forms of pre and post treatment. The protein level of cleaved caspase-3, the number and density of tyrosine hydroxylase (TH) positive neurons and the microglia population in LC were studied by western blot and immunohistochemistry respectively. Morphological changes of LC nucleus and its neurons were also studied by using stereological analysis. The number of neurons and volume of LC was reserved in animals that had received post-RSD ExMe. Apoptosis significantly was decreased comparing to RSD and Pre-RSD animals. Melatonin post-treatment of RSD rats also decreased cleavage of caspase-3 and increased reduced glutathione content in LC. Moreover, immunohistochemistry analysis showed an increase in the number of TH positive neurons and a decrease in microglia migration. Based on our findings antioxidant properties of exogenous melatonin could play a critical role in certain types of sleep disorders. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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37. The impact of congenital uterine abnormalities on pregnancy and fertility: a literature review.
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Hosseinirad H, Yadegari P, Falahieh FM, Shahrestanaki JK, Karimi B, Afsharzadeh N, and Sadeghi Y
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- Female, Fertility, Humans, Pregnancy, Pregnancy Outcome epidemiology, Reproductive Techniques, Assisted, Urogenital Abnormalities complications, Urogenital Abnormalities epidemiology, Urogenital Abnormalities therapy, Uterus
- Abstract
Congenital abnormalities of the uterus result primarily from embryological maldevelopment of the paramesonephric ducts and have been associated with pregnancy complications, reduced fertility, and other adverse fetal outcomes. While such abnormalities are rare, affected patients should be correctly managed to improve psychological, sexual, and reproductive outcomes. This review intends to elucidate the impact of congenital uterine abnormalities on fertility and pregnancy outcomes. We also present the available management methods and discuss the role of assisted reproductive technologies (ART) to benefit affected women. This review clearly shows that although these disorders are generally not lethal, they critically impact the patient's reproductive health. The fertility rate of patients with uterine congenital abnormalities depends on the severity of the condition. Reproductive endocrinologists and infertility specialists must be considered as active parts of the interdisciplinary treatment team for such patients. ART practices are reasonably successful at managing fertility problems of women with these abnormalities.
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- 2021
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38. Functional dopaminergic neurons derived from human chorionic mesenchymal stem cells ameliorate striatal atrophy and improve behavioral deficits in Parkinsonian rat model.
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Ebrahimi V, Eskandarian Boroujeni M, Aliaghaei A, Abdollahifar MA, Piryaei A, Haghir H, and Sadeghi Y
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- Animals, Parkinson Disease, Secondary pathology, Parkinson Disease, Secondary physiopathology, Rats, Behavior, Animal physiology, Corpus Striatum pathology, Dopaminergic Neurons transplantation, Mesenchymal Stem Cells cytology, Motor Activity physiology, Neurogenesis physiology, Parkinson Disease, Secondary surgery
- Abstract
Human chorionic mesenchymal stem cells (HCMSCs) have been recognized as a desirable choice for cell therapy in neurological disorders such as Parkinson's disease (PD). Due to invaluable features of HCMSCs including their immunomodulatory and immunosuppressive properties, easily accessible and less differentiated compared to other types of MSCs, HCMSCs provide a great hope for regenerative medicine. Thus, the purpose of this study was to determine the in vitro and in vivo efficacy of HCMSCs-derived dopaminergic (DA) neuron-like cells with regard to PD. Initially, HCMSCs were isolated and underwent a 2-week DA differentiation, followed by in vitro assessments, using quantitative real-time polymerase chain reaction, immunocytochemistry, patch clamp recording, and high-performance liquid chromatography. In addition, the effects of implanted HCMSCs-derived DA neuron-like cells on the motor coordination along with stereological alterations in the striatum of rat models of PD were investigated. Our results showed that under neuronal induction, HCMSCs revealed neuron-like morphology, and expressed neuronal and DA-specific genes, together with DA release. Furthermore, transplantation of HCMSCs-derived DA neurons into the striatum of rat models of PD, augmented performance. Besides, it prevented reduction of striatal volume, dendritic length, and the total number of neurons, coupled with a diminished level of cleaved caspase-3. Altogether, these findings suggest that HCMSCs could be considered as an attractive strategy for cell-based therapies in PD., (© 2019 American Association for Anatomy.)
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- 2020
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39. Sciatic nerve injury alters the spatial arrangement of neurons and glial cells in the anterior horn of the spinal cord.
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Rashidiani-Rashidabadi A, Heidari MH, Sajadi E, Hejazi F, Fathabady FF, Sadeghi Y, Aliaghaei A, Raoofi A, Abdollahifar MA, and Farahni RM
- Abstract
The spatial arrangement of the cell is important and considered as underlying mechanism for mathematical modeling of cell to cell interaction. The ability of cells to take on the characteristics of other cells in an organism, it is important to understand the dynamical behavior of the cells. This method implements experimental parameters of the cell-cell interaction into the mathematical simulation of cell arrangement. The purpose of this research was to explore the three-dimensional spatial distribution of anterior horn cells in the rat spinal cord to examine differences after sciatic nerve injury. Sixteen Sprague-Dawley male rats were assigned to control and axotomy groups. Twelve weeks after surgery, the anterior horn was removed for first- and second-order stereological studies. Second-order stereological techniques were applied to estimate the pair correlation and cross-correlation functions using a dipole probe superimposed onto the spinal cord sections. The findings revealed 7% and 36% reductions in the mean volume and total number of motoneurons, respectively, and a 25% increase in the neuroglial cell number in the axotomized rats compared to the control rats. In contrast, the anterior horn volume remained unchanged. The results also indicated a broader gap in the pair correlation curve for the motoneurons and neuroglial cells in the axotomized rats compared to the control rats. This finding shows a negative correlation for the distribution of motoneurons and neuroglial cells in the axotomized rats. The cross-correlation curve shows a negative correlation between the motoneurons and neuroglial cells in the axotomized rats. These findings suggest that cellular structural and functional changes after sciatic nerve injury lead to the alterations in the spatial arrangement of motoneurons and neuroglial cells, finally affecting the normal function of the central nervous system. The experimental protocol was reviewed and approved by the Animal Ethics Committee of Shahid Beheshti University of Medical Sciences (approval No. IR.SBMU.MSP.REC1395.375) on October 17, 2016., Competing Interests: None
- Published
- 2019
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40. Nigral injection of 6-hydroxydopamine induces changes in spatial arrangement of striatal neuron and glial cells.
- Author
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Ebrahimi V, Aliaghaei A, Piryaei A, Haghir H, Abdollahifar MA, and Sadeghi Y
- Subjects
- Animals, Corpus Striatum cytology, Disease Models, Animal, Male, Neuroglia pathology, Neurons pathology, Rats, Rats, Sprague-Dawley, Substantia Nigra cytology, Corpus Striatum drug effects, Neuroglia drug effects, Neurons drug effects, Oxidopamine pharmacology, Parkinsonian Disorders drug therapy, Substantia Nigra drug effects, Sympatholytics pharmacology
- Abstract
In Parkinson's disease, nigral dopamine neurons are lost and the structure of the striatum is progressively degraded. These events lead to a substantial neuronal loss in the striatum, changing spatial pattern of the neurons and glial cells, and associated cellular connections. Therefore, the aim of this study was to develop a new insight into whether the Parkinson's disease causes a change in the spatial arrangement of the neurons and glial cells in the striatum. Nigral injection of 6-hydroxydopamine led to a significant reduction in the total number of the neurons, an increase in the number of striatal glial cells, and disruption in the spatial arrangement of glial and neuronal cells in the Parkinson's disease-induced group, compared to the control group. The data support the idea that in Parkinson's disease, the function of the striatum is disturbed by both the loss of neurons and an increase in the number of glial cells, culminating in the disordered spatial distribution of these cells.
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- 2019
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41. Neural differentiation of choroid plexus epithelial cells: role of human traumatic cerebrospinal fluid.
- Author
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Hashemi E, Sadeghi Y, Aliaghaei A, Seddighi A, Piryaei A, Broujeni ME, Shaerzadeh F, Amini A, and Pouriran R
- Abstract
As the key producer of cerebrospinal fluid (CSF), the choroid plexus (CP) provides a unique protective system in the central nervous system. CSF components are not invariable and they can change based on the pathological conditions of the central nervous system. The purpose of the present study was to assess the effects of non-traumatic and traumatic CSF on the differentiation of multipotent stem-like cells of CP into the neural and/or glial cells. CP epithelial cells were isolated from adult male rats and treated with human non-traumatic and traumatic CSF. Alterations in mRNA expression of Nestin and microtubule-associated protein ( MAP2 ), as the specific markers of neurogenesis, and astrocyte marker glial fibrillary acidic protein ( GFAP ) in cultured CP epithelial cells were evaluated using quantitative real-time PCR. The data revealed that treatment with CSF (non-traumatic and traumatic) led to increase in mRNA expression levels of MAP2 and GFAP . Moreover, the expression of Nestin decreased in CP epithelial cells treated with non-traumatic CSF, while treatment with traumatic CSF significantly increased its mRNA level compared to the cells cultured only in DMEM/F12 as control. It seems that CP epithelial cells contain multipotent stem-like cells which are inducible under pathological conditions including exposure to traumatic CSF because of its compositions., Competing Interests: Conflicts of interest: None declared.
- Published
- 2017
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42. Mesenchymal stem cells as an alternative for Schwann cells in rat spinal cord injury.
- Author
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Zaminy A, Shokrgozar MA, Sadeghi Y, Noroozian M, Heidari MH, and Piryaei A
- Subjects
- Animals, Axons pathology, Bone Marrow Cells cytology, Bone Marrow Cells ultrastructure, Cell Lineage, Cell Transdifferentiation, Flow Cytometry, Gene Expression Regulation, Male, Mesenchymal Stem Cells metabolism, Mesenchymal Stem Cells ultrastructure, Myelin Sheath metabolism, Nerve Regeneration, RNA, Messenger genetics, RNA, Messenger metabolism, Rats, Rats, Wistar, Recovery of Function, Schwann Cells ultrastructure, Spinal Cord pathology, Spinal Cord Injuries pathology, Spinal Cord Injuries physiopathology, Tissue Scaffolds, Mesenchymal Stem Cell Transplantation, Mesenchymal Stem Cells cytology, Schwann Cells cytology, Spinal Cord Injuries therapy
- Abstract
Background: Spinal cord has a limited capacity to repair; therefore, medical interventions are necessary for treatment of injuries. Transplantation of Schwann cells has shown a great promising result for spinal cord injury (SCI). However, harvesting Schwann cell has been limited due to donor morbidity and limited expansion capacity. Furthermore, accessible sources such as bone marrow stem cells have drawn attentions to themselves. Therefore, this study was designed to evaluate the effect of bone marrow-derived Schwann cell on functional recovery in adult rats after injury., Methods: Mesenchymal stem cells were cultured from adult rats' bone marrow and induced into Schwann cells in vitro. Differentiation was confirmed by immunocytochemistry and RT-PCR. Next, Schwann cells were seeded into collagen scaffolds and engrafted in 3 mm lateral hemisection defects. For 8 weeks, motor and sensory improvements were assessed by open field locomotor scale, narrow beam, and tail flick tests. Afterwards, lesioned spinal cord was evaluated by conventional histology and immunohistochemistry., Results: In vitro observations showed that differentiated cells had Schwann cell morphology and markers. In this study, we had four groups (n = 10 each): laminectomy, control, scaffold and scaffold + Schwann cells. Locomotor and sensory scores of cell grafted group were significantly better than control and scaffold groups. In histology, axonal regeneration and remyelination were better than control and scaffold groups., Conclusion: This study demonstrates that bone marrow-derived Schwann cells can be considered as a cell source for Schwann cells in SCI treatment.
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- 2013
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43. Effect of low-level laser therapy on skin fibroblasts of streptozotocin-diabetic rats.
- Author
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Mirzaei M, Bayat M, Mosafa N, Mohsenifar Z, Piryaei A, Farokhi B, Rezaei F, Sadeghi Y, and Rakhshan M
- Subjects
- Animals, Cell Proliferation radiation effects, Diabetes Complications radiotherapy, Diabetes Mellitus, Experimental pathology, Male, Organ Culture Techniques, Rats, Tramadol therapeutic use, Wound Healing radiation effects, Diabetes Mellitus, Experimental radiotherapy, Fibroblasts radiation effects, Low-Level Light Therapy
- Abstract
Objective: This study explored the effects of low-level laser therapy (LLLT) on cellular changes in cell culture and organ culture of skin from streptozotocin-diabetic (STZ-D) rats., Background Data: Growth of skin and its fibroblasts are impaired in diabetes. Therefore the healing of skin wounds is impaired in diabetic patients. The positive effects of LLLT on complications of diabetes in patients and animal models have been shown., Methods: Diabetes was induced in rats by streptozotocin 30 days after its injection. Two sets of skin samples were extracted from skin under sterile conditions. Fibroblasts that were extruded from the samples were proliferated in vitro, and another set of samples were cultured as organ culture. A 24-well culture medium containing Dulbecco's modified minimum essential medium was supplemented by 12% fetal bovine serum. There were five laser-treated and five sham-exposed groups. A helium-neon laser was used, and 0.9-4 J/cm(2) energy densities were applied four times to each organ culture and cell culture. The organ cultures were analyzed by light microscopy and transmission electron microscopy examinations. Cell proliferation was evaluated by dimethylthiazol-diphenyltetrazolium bromide (MTT) assay., Results: Statistical analysis revealed that 4-J/cm(2) irradiation significantly increases the fibroblast numbers compared to the sham-exposed cultures (p = 0.046)., Conclusion: It is concluded that LLLT resulted in a significant increase of fibroblast proliferation of STZ-D rats in vitro.
- Published
- 2007
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44. The effect of 30-day pretreatment with pentoxifylline on the survival of a random skin flap in the rat: an ultrastructural and biomechanical evaluation.
- Author
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Bayat M, Chelcheraghi F, Piryaei A, Rakhshan M, Mohseniefar Z, Rezaie F, Bayat M, Shemshadi H, and Sadeghi Y
- Subjects
- Animals, Biomechanical Phenomena, Blood Vessels ultrastructure, Endothelium, Vascular ultrastructure, Male, Microscopy, Electron, Transmission, Mitochondrial Swelling, Rats, Skin blood supply, Graft Survival drug effects, Hematologic Agents administration & dosage, Pentoxifylline administration & dosage, Skin ultrastructure, Surgical Flaps
- Abstract
Background: The aim of this study was to clarify the histological, ultrastructural and biomechanical effects of pentoxifylline (PTX) on the survival of random skin flaps (RSFs) in rats., Material/methods: Thirty male rats were randomly divided into experimental, sham and control groups. The experimental group received PTX 20 mg/kg/day, and the sham group received saline. A 20x70-mm RSF was made 30 days after the commencement of treatment for the three groups. PTX and saline were continued postoperatively for 7 days in the experimental and sham groups, respectively. On the seventh postoperative day, the surviving parts of the flaps were determined and examined through light and transmission electron microscopes. The wounds (incisions) on the margins of the flaps were evaluated histologically and biomechanically., Results: Analysis of variance showed that, in the experimental group, the mean of the surviving parts of the RSFs, fibroblast proliferation, collagen organization and granulation tissue of the wounds was significantly higher than in the sham and control groups (P=0.007, P=0.001, P=0.041, P=0.000, respectively). There were swollen mitochondria in the endothelium of the blood vessels of the surviving flap parts in the control and sham groups, whereas in the experimental group the mitochondria were normal., Conclusions: Thirty days of pretreatment of RSFs with PTX significantly increased the survival of the flaps. PTX appeared to have healed wounds and reversed ultrastructural changes resulting from hypoxia in the blood vessel endothelium of the flaps.
- Published
- 2006
45. Low-level laser therapy improves early healing of medial collateral ligament injuries in rats.
- Author
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Bayat M, Delbari A, Almaseyeh MA, Sadeghi Y, Bayat M, and Reziae F
- Subjects
- Animals, Dose-Response Relationship, Radiation, Male, Rats, Rats, Wistar, Tensile Strength, Low-Level Light Therapy methods, Medial Collateral Ligament, Knee injuries, Wound Healing
- Abstract
Objective: This study sought to investigate whether or not low-level laser therapy (LLLT) with a helium-neon laser increased biomechanical parameters of transected medial collateral ligament (MCL) in rats., Background Data: It has been reported that LLLT can enhance tendon healing., Methods: Thirty rats received surgical transection to their right MCL, and five were assigned as the control group. After surgery, the rats were divided into three groups: group 1 (n = 10) received LLLT with 0.01 J/cm(2) energy density per day, group 2 (n = 10) received LLLT with 1.2 J/cm(2) energy density per day, and group 3 (sham = exposed group; n = 10) received daily placebo laser with shut-down laser equipment, while the control group received neither surgery nor LLLT. Biomechanical tests were performed at 12 and 21 days after surgery. The data were analyzed by one-way analysis of variance., Results: The ultimate tensile strength (UTS) of group 2 on day 12 was significantly higher than that of groups 1 and 3. Furthermore, the UTS and energy absorption of the control (uninjured) group were significantly higher than those of the other groups., Conclusions: LLLT with a helium-neon laser is effective for the early improvement of the ultimate tensile strength of medial collateral ligament injuries.
- Published
- 2005
- Full Text
- View/download PDF
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