77 results on '"Sanchez-Sanchez, R."'
Search Results
2. Gamma-ray measurements of the activated target components in a cyclotron used for positron emission tomography
- Author
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Jurado Vargas, M., Carbajo Chávez, J.C., Sánchez Sánchez, R., and Sosa, C.
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- 2020
- Full Text
- View/download PDF
3. Combined positive axillary lymph node marking with iodine-125 seeds and sentinel lymph node biopsy in breast cancer patients treated with neoadjuvant chemotherapy
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Rebollo Aguirre, A.C., Sánchez Sánchez, R., González Jiménez, A.D., Culiañez Casas, M., Mendoza Arnau, I., Rashki, M., Rudolphi Solero, T., and Martínez Meca, S.
- Published
- 2020
- Full Text
- View/download PDF
4. Surgical clips vs. iodine-125 (125I) seeds for marking the location of nonpalpable malignant breast lesions: preliminary results
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Mendoza Arnau, I., Sánchez Sánchez, R., Culiáñez Casas, M., Rebollo Aguirre, Á.C., González Jiménez, A.D., and Martínez Meca, S.
- Published
- 2020
- Full Text
- View/download PDF
5. 125I radioactive seed localization for non-palpable lesions in breast cancer
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Sánchez Sánchez, R., González Jiménez, A.D., Rebollo Aguirre, A.C., Mendoza Arnau, I., Menjón Beltrán, S., Vergara Alcaide, M.E., Osorio Ceballos, J.L., and Llamas Elvira, J.M.
- Published
- 2019
- Full Text
- View/download PDF
6. Diagnostic Imaging in Dermatology: Utility of PET-CT in Cutaneous Melanoma
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Sánchez-Sánchez, R., Serrano-Falcón, C., and Rebollo Aguirre, A.C.
- Published
- 2015
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- View/download PDF
7. Removal of triazine herbicides from aqueous systems by a biofilm reactor continuously or intermittently operated
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Sánchez-Sánchez, R., Ahuatzi-Chacón, D., Galíndez-Mayer, J., Ruiz-Ordaz, N., and Salmerón-Alcocer, A.
- Published
- 2013
- Full Text
- View/download PDF
8. Positron tomography with 18F-fluorodeoxyglucose in the preoperative evaluation of gallbladder lesions suspicious of malignancy: Diagnostic utility and clinical impact
- Author
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Ramos-Font, C., Gómez Río, M., Rodríguez-Fernández, A., Sánchez Sánchez, R., and Llamas Elvira, J.M.
- Published
- 2011
- Full Text
- View/download PDF
9. Utility of PET/CT for mediastinal staging of non-small cell lung cancer in stage III (N2)
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Sánchez Sánchez, R., Rodríguez Fernández, A., Gómez Río, M., Alkurdi Martínez, A., Castellón Rubio, V.E., Ramos Font, C., Sánchez-Palencia Ramos, A., Delgado Pérez, J.R., and Llamas Elvira, J.M.
- Published
- 2011
- Full Text
- View/download PDF
10. Ovulation rate, embryo mortality and intrauterine growth retardation in obese swine with gene polymorphisms for leptin and melanocortin receptors
- Author
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Gonzalez-Añover, P., Encinas, T., Torres-Rovira, L., Pallares, P., Muñoz-Frutos, J., Gomez-Izquierdo, E., Sanchez-Sanchez, R., and Gonzalez-Bulnes, A.
- Published
- 2011
- Full Text
- View/download PDF
11. Radioguided surgery of intestinal carcinoid tumor relapse. Role of SPECT-CT
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Serrano Vicente, J., Domínguez Grande, M.L., Infante De La Torre, J.R., Duran Barquero, C., Garcia Bernardo, L., Rayo Madrid, J.I., Pérez Andrés, I., and Sánchez Sánchez, R.
- Published
- 2010
- Full Text
- View/download PDF
12. Optimization of sentinel lymph node biopsy in breast cancer by intraoperative axillary palpation
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Serrano Vicente, J., Infante de la Torre, J.R., Domínguez Grande, M.L., García Bernardo, L., Durán Barquero, C., Rayo Madrid, J.I., Sánchez Sánchez, R., Correa Antúnez, M.I., Amaya Lozano, J.L., and Conde Martín, A.F.
- Published
- 2010
- Full Text
- View/download PDF
13. Negative Predictive Value of the SPECT with 123I Loflupane in movement disorders
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Serrano Vicente, J., García Bernardo, L., Durán Barquero, C., Constantino Silva, A., Infante de la Torre, J.R., Domínguez Grande, M.L., Rayo Madrid, J.I., Sánchez Sánchez, R., and Durán Herrera, C.
- Published
- 2009
- Full Text
- View/download PDF
14. Accuracy of in vivo and ex vivo ultrasonographic evaluation of ovarian follicles and corpora lutea in sows
- Author
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Gonzalez-Añover, P., Encinas, T., Gomez-Izquierdo, E., Sanz, E., Sanchez-Sanchez, R., and Gonzalez-Bulnes, A.
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- 2009
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- View/download PDF
15. Prevalence of malnutrition and sarcopenia in Sedreno-2 study according to GLIM And EWGSOP2 criteria
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Zugasti Murillo, A., Petrina Jáuregui, M.E., Villazon González, F., Ripa Ciaurriz, M.C., Calles Romero, L., Chinchetru Ranedo, M.J., Riestra Fernández, M., Rengel Jiménez, J., Sánchez Sánchez, R., Valle Rodríguez, S., Diez Muñiz, M., Agorreta Ruiz, J., Guimón Bardesi, A., Larrañaga Unanue, I., Elías Martínez, E., Zubillaga, I.P., Irigaray Echarri, A., Salsamendi Pérez, J.L., Abínzano Guillén, M.L., and Olariaga Sarasola, O.
- Published
- 2020
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- View/download PDF
16. Implantation of a heterologous dermo-epidermal skin substitute in a patient with deep dermal burn that enhances biomechanical and functional recovery: Case report
- Author
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Ornelas-Flores, M.C., García-López, J., Melgarejo-Ramírez, Y., Sánchez-Sánchez, R., Leyva-Gómez, G., Zacaula-Juárez, N., González-Mendoza, O., Manzo-Castrejón, H.A., Ferreira-Aparicio, F.E., Márquez-Gutiérrez, E., Martínez-Pardo, M.E., Velasquillo-Martínez, M.C., Ibarra-Ponce de León, J.C., and Brena-Molina, A.M.
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- 2018
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17. Prepuberal evolution of plasma leptin levels in gilts of thrifty genotype (Iberian pig) and lean commercial crosses (Large White × Landrace)
- Author
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Gonzalez-Añover, P., Vigo, E., Encinas, T., Torres-Rovira, L., Pallares, P., Gomez-Izquierdo, E., Sanchez-Sanchez, R., Mallo, F., and Gonzalez-Bulnes, A.
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- 2012
- Full Text
- View/download PDF
18. Coexistence of Two Different Types of Lymphoma in a Patient with Sjögren's Syndrome. The Usefulness of the PET-CT
- Author
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Infante De La Torre, J.R., Durán Barquero, C., García Bernardo, L., Groiss, J., Rayo Madrid, J.I., Serrano Vicente, J., Domínguez Grande, M.L., and Sánchez Sánchez, R.
- Published
- 2012
- Full Text
- View/download PDF
19. Patterns of corpora lutea growth and progesterone secretion in sows with thrifty genotype and leptin resistance due to leptin receptor gene polymorphisms (Iberian pig)
- Author
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Gonzalez-Añover, P., Encinas, T., Torres-Rovira, L., Sanz, E., Pallares, P., Ros, J. M., Gomez-Izquierdo, E., Sanchez-Sanchez, R., and Gonzalez-Bulnes, A.
- Subjects
endocrine system ,urogenital system ,reproductive and urinary physiology ,hormones, hormone substitutes, and hormone antagonists - Abstract
The current study aimed to compare luteal function, as measured by corpora lutea dynamics and progesterone secretion, in 10 sows with obesity/leptin resistance genotype (Iberian pig) and 10 females of lean commercial crosses (Large White×Landrace). In all the animals, the oestrous cycle was synchronized with progestagens, and ovulation was induced by exogenous gonadotrophins. Thereafter, number and size of follicles and plasma oestradiol concentration were determined at oestrus detection, and number and size of corpora lutea and progesterone concentration were evaluated from Day 4 to 12 of the cycle. There were no differences between genotypes in follicle population and oestradiol concentration, and ovulation rate (15.2±1.3 in Iberian vs 12.7±1.8 in LWxL sows); however, there was a higher percentage of Iberian than control sows showing luteal cysts (66.7% vs 30%, respectively; p
- Published
- 2011
20. Advanced onset of puberty in gilts of thrifty genotype (Iberian pig)
- Author
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Gonzalez-Añover, P., Encinas, T., Gomez-Izquierdo, E., Sanz, E., Letelier, C. A., Torres-Rovira, L., Pallares, P., Sanchez-Sanchez, R., and Gonzalez-Bulnes, A.
- Subjects
humanities - Abstract
Contents The Iberian pig is an autochthonous breed of the Mediterranean area, reared extensively in the central and southern areas of Spain and Portugal and that is known worldwide for the production of Iberian ham. The characteristics of the Iberian ham are related to its abundance of intramuscular fat, owing to the high capacity of the pig to accumulate fat under its skin and between the muscular fibres. This ability to store excess fat enables survival during periods of scarcity and it has been found in other antique animal breeds and even in humans, being named as the thrifty genotype. The reproductive management of the Iberian pig, in spite of a lack of accurate information unlike in other swine breeds, is based on the assumption of lower reproductive precocity and efficiency than the modern commercial breeds. The current study characterized and compared the onset of puberty in gilts of Iberian breed and meat commercial crosses (Large White × Landrace) reared in the same conditions by assessing weekly plasma progesterone profiles. At the end of the experimental period, when the gilts became 180 days old, the percentage of Iberian females that had reached puberty was 72.2%, with a mean age and weight of 160.5 ± 2.6 days and 92.9 ± 4.7 kg, respectively. The percentage of Large White × Landrace cross-breed females reaching puberty at 180 days was only 15% (p < 0.05). The mean age and weight were 165.0 ± 4.1 days and 107.8 ± 2.2 kg (p < 0.05), respectively. Thus, converse to the traditional assumption, onset of puberty was advanced in Iberian gilts. These results are important for the reproductive management of the Iberian pig but, at the same time, may set the basis for future studies on the metabolism-reproduction link and, specifically, on the possible relationship between earlier accumulation of fat and attainment of puberty. © 2009 Blackwell Verlag GmbH.
- Published
- 2010
21. PET/CT: Focal lung uptake of 18F-fluordeoxyglucose on PET but no structural alterations on CT
- Author
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Sánchez-Sánchez, R., Rodríguez-Fernández, A., Ramírez-Navarro, Á., Gómez-Río, M., Ramos-Font, C., and Llamas-Elvira, J.M.
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- 2010
- Full Text
- View/download PDF
22. Maternal diet-induced obesity in swine with leptin resistance modifies puberty and pregnancy outputs of the adult offspring.
- Author
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Gonzalez-Bulnes, A., Astiz, S., Sanchez-Sanchez, R., Perez-Solana, M., and Gomez-Fidalgo, E.
- Abstract
The assessment of reproductive features (puberty, fertility and prolificacy) in female Iberian pigs indicates that exposition to intrauterine maternal malnutrition, either by deficiency or excess, is associated with juvenile obesity and a significantly earlier age of puberty onset. At adulthood, prenatal exposition to undernutrition affects reproductive outputs by diminishing prolificacy, an effect that was not found in females exposed to prenatal overnutrition. [ABSTRACT FROM PUBLISHER]
- Published
- 2013
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- View/download PDF
23. In vitro Release of Ovarian Progesterone is Decreased During the Oestrous Cycle and Pregnancy of Swine with Obesity/Leptin Resistance.
- Author
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Astiz, S, Gonzalez‐Bulnes, A, Perez‐Solana, ML, Sanchez‐Sanchez, R, and Torres‐Rovira, L
- Subjects
IN vitro studies ,PROGESTERONE ,OVARIAN follicle ,ESTRUS ,MAMMAL reproduction ,SWINE ,OBESITY ,LEPTIN - Abstract
Contents Previous studies indicate that reproductive prolificacy of obese swine breeds is markedly influenced by embryo losses in early pregnancy. In such period, adequate secretion of progesterone (P4) by the ovary is essential for pregnancy success. This study analyses the luteal functionality during the oestrous cycle and early pregnancy of Iberian sows and Large White x Landrace females, in terms of P4 secretion after in vitro culture of luteal tissue stimulated or not with luteinizing hormone ( LH). The secretion of progesterone (expressed in ng/mg of luteal tissue or ng/mg LT) of the corpora lutea of obese Iberian swine was always hampered when compared to lean genotypes, either during early oestrous cycle (110.7 ± 37.8 vs 259.7 ± 10.2 ng/mg LT; p < 0.0001), late oestrous cycle (49.0 ± 3.5 vs 75.92 ± 7.14 ng/mg LT; p < 0.0001) or early pregnancy (38.4 ± 2.1 vs 70.7 ± 5.3 ng/mg LT; p < 0.0001). The differences in basal P4 secretion remained after stimulation with LH. Finally, P4 secretion during early pregnancy of Iberian sows decreased with age and, hence, with obesity features (46.6 ± 4.2 vs 65.5 ± 4.8 ng/mg LT; p < 0.001). In conclusion, the results of the present study provide convincing evidence of a reduced luteal function during oestrous cycle and early pregnancy of sows with obesity/leptin resistance like Iberian sows, which may contribute to the low reproductive efficiency reported in this breed. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
24. Gender-specific early postnatal catch-up growth after intrauterine growth retardation by food restriction in swine with obesity/leptin resistance.
- Author
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Gonzalez-Bulnes, A., Ovilo, C., Lopez-Bote, C. J., Astiz, S ., Ayuso, M ., Perez-Solana, M. L., Sanchez-Sanchez, R., and Torres-Rovira, L.
- Subjects
FETAL development ,LABORATORY swine ,LEPTIN ,OBESITY ,URINALYSIS ,NUTRITIONAL requirements - Abstract
The effects of undernutrition during pregnancy on prenatal and postnatal development of the offspring were evaluated in sows with obesity/leptin resistance. Females were fed, from day 35 of pregnancy onwards, a diet fulfilling either 100% (group control, n = 10) or 50% of the nutritional requirements (group underfed, n=10). In the control group, maternal body weight increased during pregnancy (P<0.05) while it decreased or remained steady in the underfed group. At days 73 and 100 of gestation, plasma triglycerides were lower but urea levels were higher in restricted than in control sows (P<0.05 for both). Assessment of the offspring indicated that the trunk diameter was always smaller in the restricted group (P< 0.01 at day 50, P< 0.005 at days 75 and 100 and P< 0.0001 at birth) while head measurements were similar through pregnancy, although smaller in the restricted than in the control group at birth (P< 0.05). Newborns from restricted sows were also lighter than offspring from control females (P<0.01) and had higher incidence of growth retardation (P<0.01). Afterwards, during lactation, early postnatal growth in restricted piglets was modulated by gender. At weaning, males from restricted sows were still lighter than their control counterparts (P<0.05), while females from control and underfed sows were similar. Thus, the current study indicates a gender-related differential effect in the growth patterns of the piglets, with females from restricted sows evidencing catch-up growth to neutralise prenatal retardation and reaching similar development than control counterparts. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
25. Diet-Induced Swine Model with Obesity/Leptin Resistance for the Study of Metabolic Syndrome and Type 2 Diabetes.
- Author
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Torres-Rovira, L., Astiz, S., Caro, A., Lopez-Bote, C., Ovilo, C., Pallares, P., Perez-Solana, M. L., Sanchez-Sanchez, R., and Gonzalez-Bulnes, A.
- Subjects
LABORATORY swine ,OBESITY ,PHYSIOLOGICAL effects of leptin ,METABOLIC syndrome ,TYPE 2 diabetes ,DEFICIENCY diseases ,INSULIN resistance - Published
- 2012
- Full Text
- View/download PDF
26. Patterns of Corpora Lutea Growth and Progesterone Secretion in Sows with Thrifty Genotype and Leptin Resistance due to Leptin Receptor Gene Polymorphisms (Iberian Pig).
- Author
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Gonzalez-Añover, P, Encinas, T, Torres-Rovira, L, Sanz, E, Pallares, P, Ros, JM, Gomez-Izquierdo, E, Sanchez-Sanchez, R, and Gonzalez-Bulnes, A
- Subjects
CORPUS luteum ,PROGESTERONE ,SOWS ,GENETIC polymorphisms ,LEPTIN ,PEPTIDE receptors ,ESTRUS ,COMPARATIVE studies ,REPRODUCTION - Abstract
Contents The current study aimed to compare luteal function, as measured by corpora lutea dynamics and progesterone secretion, in 10 sows with obesity/leptin resistance genotype (Iberian pig) and 10 females of lean commercial crosses (Large White × Landrace). In all the animals, the oestrous cycle was synchronized with progestagens, and ovulation was induced by exogenous gonadotrophins. Thereafter, number and size of follicles and plasma oestradiol concentration were determined at oestrus detection, and number and size of corpora lutea and progesterone concentration were evaluated from Day 4 to 12 of the cycle. There were no differences between genotypes in follicle population and oestradiol concentration, and ovulation rate (15.2 ± 1.3 in Iberian vs 12.7 ± 1.8 in LWxL sows); however, there was a higher percentage of Iberian than control sows showing luteal cysts (66.7% vs 30%, respectively; p < 0.05). In both breeds, both total luteal area and plasma progesterone concentration grew linearly from Day 4 to 8 (p < 0.01) and remained more stable between Days 8 and 12, without significant differences between genotypes. In conclusion, current study supports that ovulatory processes and luteal functionality are not the main limiting factors for prolificacy in a pig model of leptin resistance and obesity. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
27. Advanced Onset of Puberty in Gilts of Thrifty Genotype (Iberian Pig).
- Author
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Gonzalez-Añover, P, Encinas, T, Gomez-Izquierdo, E, Sanz, E, Letelier, CA, Torres-Rovira, L, Pallares, P, Sanchez-Sanchez, R, and Gonzalez-Bulnes, A
- Subjects
PUBERTY ,SOWS ,ANIMAL breeds ,ANIMAL reproduction ,PROGESTERONE ,ANIMAL breeding ,METABOLISM - Abstract
The Iberian pig is an autochthonous breed of the Mediterranean area, reared extensively in the central and southern areas of Spain and Portugal and that is known worldwide for the production of Iberian ham. The characteristics of the Iberian ham are related to its abundance of intramuscular fat, owing to the high capacity of the pig to accumulate fat under its skin and between the muscular fibres. This ability to store excess fat enables survival during periods of scarcity and it has been found in other antique animal breeds and even in humans, being named as the thrifty genotype. The reproductive management of the Iberian pig, in spite of a lack of accurate information unlike in other swine breeds, is based on the assumption of lower reproductive precocity and efficiency than the modern commercial breeds. The current study characterized and compared the onset of puberty in gilts of Iberian breed and meat commercial crosses (Large White × Landrace) reared in the same conditions by assessing weekly plasma progesterone profiles. At the end of the experimental period, when the gilts became 180 days old, the percentage of Iberian females that had reached puberty was 72.2%, with a mean age and weight of 160.5 ± 2.6 days and 92.9 ± 4.7 kg, respectively. The percentage of Large White × Landrace cross-breed females reaching puberty at 180 days was only 15% (p < 0.05). The mean age and weight were 165.0 ± 4.1 days and 107.8 ± 2.2 kg (p < 0.05), respectively. Thus, converse to the traditional assumption, onset of puberty was advanced in Iberian gilts. These results are important for the reproductive management of the Iberian pig but, at the same time, may set the basis for future studies on the metabolism-reproduction link and, specifically, on the possible relationship between earlier accumulation of fat and attainment of puberty. [ABSTRACT FROM AUTHOR]
- Published
- 2010
- Full Text
- View/download PDF
28. Inguinal herniation of a bladder diverticulum, diagnosed by PET-CT
- Author
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Durán Barquero, C., Serrano Vicente, J., García Bernardo, L., Domínguez Grande, M.L., Rayo Madrid, J.I., Infante de la Torre, J.R., and Sánchez Sánchez, R.
- Published
- 2010
- Full Text
- View/download PDF
29. Plasma Leptin, Ghrelin and Indexes of Glucose and Lipid Metabolism in Relation to the Appearance of Post-Weaning Oestrus in Mediterranean Obese Sows (Iberian Pig).
- Author
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Torres-Rovira, L, Pallares, P, Vigo, E, Gonzalez-Añover, P, Sanchez-Sanchez, R, Mallo, F, and Gonzalez-Bulnes, A
- Subjects
MAMMAL reproduction ,SWINE ,LIPID metabolism ,LEPTIN ,GHRELIN ,GLUCOSE ,ESTRUS ,OBESITY in animals ,LACTATION - Abstract
Iberian pig is the most abundant Mediterranean swine. The lack of knowledge of the reproductive physiology of Mediterranean genotypes, with predisposition to obesity, led us to evaluate the influence of body condition and metabolic status at weaning on the resumption of follicular growth and the appearance of post-weaning oestrus. Females failing to display post-weaning oestrus showed a high decrease in backfat mass during lactation; backfat depth at weaning was therefore lower than in sows becoming in oestrus. Females not bearing oestrus behaviour showed lower plasma leptin levels and higher ghrelin concentrations at weaning. Moreover, these sows evidenced dyslipidemic profile (increased triglyceridemia and cholesterolemia) and mobilization of fat reserves. Hence, changes in metabolic regulation of Iberian pigs may originate large effects on the resumption of ovulatory activity after weaning. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
30. P.161 Reconstruction of the total lower lip, chin and mandible with the composite radial forearm-palmaris longus and fibular osteocutaneous free flaps
- Author
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Garcia-Montesinos, B., Sanchez Sanchez, R., Vazquez Marcos, V., Garcia Reija, F., and Saiz Bustillo, R.
- Published
- 2005
- Full Text
- View/download PDF
31. Synovial sarcoma of the masticator space: report of a case.
- Author
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Dominguez MF, Sanchez Sanchez R, Gonzalez FS, Perticone MA, Gonzalez JM, and Mancha de la Plata M
- Published
- 2011
32. Reproductive, endocrine and metabolic feto-maternal features and placental gene expression in a swine breed with obesity/leptin resistance
- Author
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Gonzalez-Bulnes, A., Torres-Rovira, L., Ovilo, C., Astiz, S., Gomez-Izquierdo, E., Gonzalez-Añover, P., Pallares, P., Perez-Solana, M.L., and Sanchez-Sanchez, R.
- Subjects
- *
OBESITY , *GENE expression , *MAMMAL reproduction , *SWINE , *METABOLISM , *LEPTIN , *MATERNAL-fetal exchange , *REPRODUCTION endocrinology , *SWINE breeds - Abstract
Abstract: The current study was conducted in a swine breed (Iberian pig) with a genotype that predisposed the pig to obesity. The aim of the study was to determine the morphological, metabolomic and endocrine features of early conceptuses and to elucidate how placental gene expression (related to placentation, angiogenesis and fetal nutrition), maternal hormones and the metabolome affect the fetal environment and fetal growth. Conceptus viability and growth were found to be related to maternal endocrine (plasma progesterone levels) and metabolic features (plasma levels of leptin, cholesterol, HDL-c, LDL-c and triglycerides). These features were related to the placental expression of the vascular endothelial growth factor A (VEGFA) and leptin (LEP) genes, the placental efficiency and, thus, the nutrition and the metabolism of the fetus (availability of glucose, triglycerides and cholesterol, as HDL-c). Viability of conceptuses in females with evidence of dyslipidemia (low plasma levels of total cholesterol due to low HDL-c concentration but high levels of triglycerides) was diminished. The availability of nutrients and metabolic substrates to the conceptus was also affected in females with higher fat deposition and evidence of dyslipidemia. In conclusion, the conceptus viability and growth appear to be strongly related to maternal metabolic features and, thus, affected in females with alterations in lipid metabolism. [Copyright &y& Elsevier]
- Published
- 2012
- Full Text
- View/download PDF
33. Preovulatory follicle dynamics and ovulatory efficiency in sows with thrifty genotype and leptin resistance due to leptin receptor gene polymorphisms (Iberian pig)
- Author
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Gonzalez-Añover, P., Encinas, T., Sanz, E., Letelier, C.A., Torres-Rovira, L., de Mercado, E., Pallares, P., Sanchez-Sanchez, R., and Gonzalez-Bulnes, A.
- Subjects
- *
OVULATION , *LEPTIN , *GENETIC polymorphisms , *GONADOTROPIN , *OBESITY , *MAMMAL reproduction , *SWINE , *OVARIAN follicle - Abstract
Abstract: This study compares follicular function and ovulatory efficiency in 20 sows with obesity/leptin resistance genotype (Iberian pig) and 20 females of lean commercial crosses (Large White×Landrace; LW×L). Estrous cycle was synchronized with progestagens; ovulation was induced with eCG and hCG, in half of the females of each group, to determine its effect. In females of both breeds not treated with gonadotropins, the number of follicles larger than 4.9mm and the estradiol secretion increased throughout the follicular phase (P <0.05); estradiol values were similar at estrus detection (22.5±1.2 vs. 26.5±0.6pg/ml respectively, for Iberian and LW×L sows). Moreover, ovulation rate was higher in Iberian pigs (15.3±1.3 CLs) than in LW×L (10.2±1.3 CLs; P <0.05), with mean progesterone values being 18.1±0.7ng/ml in Iberian and 16.8±0.6ng/ml in LW×L pigs. Thus, the preovulatory follicular growth and the ovulatory efficiency seem not to be the main limiting factors for reproductive efficiency in Iberian swine. The gonadotropins induced a significant increase, when compared to untreated females (P <0.05), in the number of follicles larger than 4.9mm growing throughout the follicular phase; however, estradiol values at estrus were lower (P <0.05) in both breeds (9.2±0.7pg/ml in Iberian vs. 8.6±0.8pg/ml in LW×L), when compared with the nontreated animals, which suggests defective follicular function after gonadotropin stimulation. There were also no differences between genotypes in ovulation rate (15.2±1.3 vs. 12.7±1.8) and progesterone secretion (21.2±0.8ng/ml in Iberian and 20.9±0.7ng/ml in LW×L sows) in the treated animals. In conclusion, the current findings indicate that preovulatory follicular growth and ovulatory efficiency are not main limiting factors for prolificacy in a pig model of leptin resistance and obesity. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
34. Dysregulation of RNA-Exosome machinery is directly linked to major cancer hallmarks in prostate cancer: Oncogenic role of PABPN1.
- Author
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Sáez-Martínez P, Porcel-Pastrana F, Montero-Hidalgo AJ, Lozano de la Haba S, Sanchez-Sanchez R, González-Serrano T, Gómez-Gómez E, Martínez-Fuentes AJ, Jiménez-Vacas JM, Gahete MD, and Luque RM
- Subjects
- Male, Humans, Exosome Multienzyme Ribonuclease Complex, Cell Line, Tumor, Gene Expression Regulation, Neoplastic, Neoplasm Recurrence, Local, RNA, Messenger, Poly(A)-Binding Protein I metabolism, Exosomes metabolism, Prostatic Neoplasms pathology
- Abstract
Novel biomarkers and therapeutic strategies for prostate-cancer (PCa) are required to overcome its lethal progression. The dysregulation/implication of the RNA-Exosome-complex (REC; cellular machinery controlling the 3'-5'processing/degradation of most RNAs) in different cancer-types, including PCa, is poorly known. Herein, different cellular/molecular/preclinical approaches with human PCa-samples (tissues and/or plasma of 7 independent cohorts), and in-vitro/in-vivo PCa-models were used to comprehensively characterize the REC-profile and explore its role in PCa. Moreover, isoginkgetin (REC-inhibitor) effects were evaluated on PCa-cells. We demonstrated a specific dysregulation of the REC-components in PCa-tissues, identifying the Poly(A)-Binding-Protein-Nuclear 1 (PABPN1) factor as a critical regulator of major cancer hallmarks. PABPN1 is consistently overexpressed in different human PCa-cohorts and associated with poor-progression, invasion and metastasis. PABPN1 silencing decreased relevant cancer hallmarks in multiple PCa-models (proliferation/migration/tumourspheres/colonies, etc.) through the modulation of key cancer-related lncRNAs (PCA3/FALEC/DLEU2) and mRNAs (CDK2/CDK6/CDKN1A). Plasma PABPN1 levels were altered in patients with metastatic and tumour-relapse. Finally, pharmacological inhibition of REC-activity drastically inhibited PCa-cell aggressiveness. Altogether, the REC is drastically dysregulated in PCa, wherein this novel molecular event/mechanism, especially PABPN1 alteration, may be potentially exploited as a novel prognostic and therapeutic tool for PCa., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.)
- Published
- 2024
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35. Antiproliferative and Antimigratory Activity of Poly-gallic Acid in Cancer Cell Lines.
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Hernandez-Valencia CG, Rodriguez-Martinez G, Carriles-Perez AM, Gonzalez-Perez D, Ortega-Sanchez C, Andonegui-Elguera MA, Zamudio-Cuevas Y, Fernandez-Torres J, Hernandez-Valdepena MA, Gimeno M, and Sanchez-Sanchez R
- Subjects
- Male, Humans, Lysine, Trametes, MCF-7 Cells, Arginine pharmacology, Cell Proliferation, Gallic Acid pharmacology, Prostatic Neoplasms pathology, Salicylates
- Abstract
Background/aim: Enzyme-mediated grafting of poly (gallic acid) (PGAL) and L-arginine and a-L-lysine onto PGAL produces reactive oxygen species (ROS)-suppressor multiradical molecules with low cytotoxicity, high thermostability and water solubility with cancer treatment potential. This study examined the anticancer effects of these molecules in hepatic (HepG2, ATCC HB-8065), breast (MCF7, ATCC HTB-22), and prostate (PC-3, ATCC CRL-1435 and DU 145, ATCC HTB-81) cancer cell lines, as well as in fibroblasts from healthy human skin as control cells., Materials and Methods: PGAL was synthesized by the oxidative polymerization of the naturally abundant GA using laccase from Trametes versicolor. Insertions of amino acids L-arginine and α-L-lysine on the PGAL chain were carried out by microwave. The cells of dermal fibroblast (Fb) were obtained from primary skin cultures and isolated from skin biopsies. The cancer cells lines of hepatic (HepG2), breast (MCF7), and prostate (PC-3, DU 145) were obtained from ATCC. The viability of the cancer cells and the primary culture was obtained by the MTT assay. Proliferation was demonstrated by crystal violet assay. Cell migration was determined by Wound healing assay. Finally, cell cycle analysis was carried out with cells., Results: The results show that 200 μg/ml of PGAL cultured in vitro with prostate cancer cells decreased viability, proliferation, and migration, as well as arrested cells in the G
1 and S phases of the cell cycle. In contrast, the dermal fibroblasts and the hepatic line remained unaffected. The random grafting of L-Arg and a-L-Lys onto the PGAL chain also decreased the viability of prostate cancer cells., Conclusion: PGAL and PGAL-grafted amino acids are potential adjuvants for prostate cancer treatment, with improved physicochemical characteristics compared to GA., (Copyright © 2024 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)- Published
- 2024
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36. Does Telotristat Have a Role in Preventing Carcinoid Heart Disease?
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Herrera-Martínez AD, Fuentes-Fayos AC, Sanchez-Sanchez R, Montero AJ, Sarmento-Cabral A, Gálvez-Moreno MA, Gahete MD, and Luque RM
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- Humans, Octreotide pharmacology, Octreotide therapeutic use, Serotonin, Fibrosis, Carcinoid Heart Disease drug therapy, Neuroendocrine Tumors drug therapy, Phenylalanine analogs & derivatives, Pyrimidines
- Abstract
Carcinoid heart disease (CHD) is a frequent and life-threatening complication in patients with carcinoid tumors. Its clinical management is challenging is some cases since serotonin-induced valve fibrosis leads to heart failure. Telotristat is an inhibitor of tryptophan-hydroxylase (TPH), a key enzyme in serotonin production. Telotristat use in patients with carcinoid syndrome and uncontrollable diarrhea under somatostatin analogs is approved, but its specific role in patients with CHD is still not clear. IN this context, we aimed to explore the effect of telotristat in heart fibrosis using a mouse model of serotonin-secreting metastasized neuroendocrine neoplasm (NEN). To this aim, four treatment groups (n = 10/group) were evaluated: control, monthly octreotide, telotristat alone, and telotristat combined with octreotide. Plasma serotonin and NT-proBNP levels were determined. Heart fibrosis was histologically evaluated after 6 weeks of treatment or when an individual mouse's condition was close to being terminal. Heart fibrosis was observed in all groups. Non-significant reductions in primary tumor growth were observed in all of the treated groups. Feces volume was increased in all groups. A non-significant decrease in feces volume was observed in the octreotide or telotristat-treated groups, while it was significantly reduced with the combined treatment at the end of the study compared with octreotide (52 g reduction; p < 0.01) and the control (44.5 g reduction; p = 0.05). Additionally, plasma NT-proBNP decreased in a non-significant, but clinically relevant, manner in the octreotide (28.2% reduction), telotristat (45.9% reduction), and the octreotide + telotristat (54.1% reduction) treatment groups. No significant changes were observed in plasma serotonin levels. A similar non-significant decrease in heart valve fibrosis was observed in the three treated groups. In conclusion, Telotristat alone and especially in combination with octreotide decreases NT-proBNP levels in a mouse model of serotonin-secreting metastasized NEN, when compared with the control and octreotide, but its effect on heart valve fibrosis (alone and in combination) was not superior to octreotide in monotherapy.
- Published
- 2024
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37. Adults with metabolically healthy overweight or obesity present more brown adipose tissue and higher thermogenesis than their metabolically unhealthy counterparts.
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Jurado-Fasoli L, Sanchez-Delgado G, Alcantara JMA, Acosta FM, Sanchez-Sanchez R, Labayen I, Ortega FB, Martinez-Tellez B, and Ruiz JR
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- Young Adult, Humans, Female, Obesity diagnostic imaging, Obesity metabolism, Thermogenesis, Positron Emission Tomography Computed Tomography methods, Cold Temperature, Fluorodeoxyglucose F18 metabolism, Energy Metabolism, Overweight metabolism, Adipose Tissue, Brown diagnostic imaging, Adipose Tissue, Brown metabolism
- Abstract
Background: There is a subset of individuals with overweight/obesity characterized by a lower risk of cardiometabolic complications, the so-called metabolically healthy overweight/obesity (MHOO) phenotype. Despite the relatively higher levels of subcutaneous adipose tissue and lower visceral adipose tissue observed in individuals with MHOO than individuals with metabolically unhealthy overweight/obesity (MUOO), little is known about the differences in brown adipose tissue (BAT)., Methods: This study included 53 young adults (28 women) with a body mass index (BMI) ≥25 kg/m
2 which were classified as MHOO (n = 34) or MUOO (n = 19). BAT was assessed through a static18 F-FDG positron emission tomography/computed tomography scan after a 2-h personalized cooling protocol. Energy expenditure, skin temperature, and thermal perception were assessed during a standardized mixed meal test (3.5 h) and a 1-h personalized cold exposure. Body composition was assessed by dual-energy x-ray absorptiometry, energy intake was determined during an ad libitum meal test and dietary recalls, and physical activity levels were determined by a wrist-worn accelerometer., Findings: Participants with MHOO presented higher BAT volume (+124%, P = 0.008), SUVmean (+63%, P = 0.001), and SUVpeak (+133%, P = 0.003) than MUOO, despite having similar BAT mean radiodensity (P = 0.354). In addition, individuals with MHOO exhibited marginally higher meal-induced thermogenesis (P = 0.096) and cold-induced thermogenesis (+158%, P = 0.050). Moreover, MHOO participants showed higher supraclavicular skin temperature than MUOO during the first hour of the postprandial period and during the cold exposure, while no statistically significant differences were observed in other skin temperature parameters. We observed no statistically significant differences between MHOO and MUOO in thermal perception, body composition, outdoor ambient temperature exposure, resting metabolic rate, energy intake, or physical activity levels., Interpretation: Adults with MHOO present higher BAT volume and activity than MUOO. The higher meal- and cold-induced thermogenesis and cold-induced supraclavicular skin temperature are compatible with a higher BAT activity. Overall, these results suggest that BAT presence and activity might be linked to a healthier phenotype in young adults with overweight or obesity., Funding: See acknowledgments section., Competing Interests: Declaration of interests None., (Copyright © 2023 The Author(s). Published by Elsevier B.V. All rights reserved.)- Published
- 2024
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38. Hippocampal subfield abnormalities and biomarkers of pathologic brain changes: from SARS-CoV-2 acute infection to post-COVID syndrome.
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Díez-Cirarda M, Yus-Fuertes M, Sanchez-Sanchez R, Gonzalez-Rosa JJ, Gonzalez-Escamilla G, Gil-Martínez L, Delgado-Alonso C, Gil-Moreno MJ, Valles-Salgado M, Cano-Cano F, Ojeda-Hernandez D, Gomez-Ruiz N, Oliver-Mas S, Benito-Martín MS, Jorquera M, de la Fuente S, Polidura C, Selma-Calvo B, Arrazola J, Matias-Guiu J, Gomez-Pinedo U, and Matias-Guiu JA
- Subjects
- Humans, Brain diagnostic imaging, Brain pathology, Hippocampus pathology, Atrophy, Syndrome, Biomarkers, SARS-CoV-2, COVID-19 pathology
- Abstract
Background: Cognitive deficits are among the main disabling symptoms in COVID-19 patients and post-COVID syndrome (PCS). Within brain regions, the hippocampus, a key region for cognition, has shown vulnerability to SARS-CoV-2 infection. Therefore, in vivo detailed evaluation of hippocampal changes in PCS patients, validated on post-mortem samples of COVID-19 patients at the acute phase, would shed light into the relationship between COVID-19 and cognition., Methods: Hippocampal subfields volume, microstructure, and perfusion were evaluated in 84 PCS patients and compared to 33 controls. Associations with blood biomarkers, including glial fibrillary acidic protein (GFAP), myelin oligodendrocyte glycoprotein (MOG), eotaxin-1 (CCL11) and neurofilament light chain (NfL) were evaluated. Besides, biomarker immunodetection in seven hippocampal necropsies of patients at the acute phase were contrasted against eight controls., Findings: In vivo analyses revealed that hippocampal grey matter atrophy is accompanied by altered microstructural integrity, hypoperfusion, and functional connectivity changes in PCS patients. Hippocampal structural and functional alterations were related to cognitive dysfunction, particularly attention and memory. GFAP, MOG, CCL11 and NfL biomarkers revealed alterations in PCS, and showed associations with hippocampal volume changes, in selective hippocampal subfields. Moreover, post mortem histology showed the presence of increased GFAP and CCL11 and reduced MOG concentrations in the hippocampus in post-mortem samples at the acute phase., Interpretation: The current results evidenced that PCS patients with cognitive sequalae present brain alterations related to cognitive dysfunction, accompanied by a cascade of pathological alterations in blood biomarkers, indicating axonal damage, astrocyte alterations, neuronal injury, and myelin changes that are already present from the acute phase., Funding: Nominative Grant FIBHCSC 2020 COVID-19. Department of Health, Community of Madrid. Instituto de Salud Carlos III through the project INT20/00079, co-funded by European Regional Development Fund "A way to make Europe" (JAMG). Instituto de Salud Carlos III (ISCIII) through Sara Borrell postdoctoral fellowship Grant No. CD22/00043) and co-funded by the European Union (MDC). Instituto de Salud Carlos III through a predoctoral contract (FI20/000145) (co-funded by European Regional Development Fund "A way to make Europe") (MVS). Fundación para el Conocimiento Madri+d through the project G63-HEALTHSTARPLUS-HSP4 (JAMG, SOM)., Competing Interests: Declaration of interests The authors declare that they have no competing interests., (Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)
- Published
- 2023
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39. Assessment of the Activity of Decoquinate and Its Quinoline- O -Carbamate Derivatives against Toxoplasma gondii In Vitro and in Pregnant Mice Infected with T. gondii Oocysts.
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Ramseier J, Imhof D, Anghel N, Hänggeli K, Beteck RM, Balmer V, Ortega-Mora LM, Sanchez-Sanchez R, Ferre I, Haynes RK, and Hemphill A
- Subjects
- Animals, Antiprotozoal Agents chemistry, Decoquinate analogs & derivatives, Decoquinate chemistry, Disease Models, Animal, Female, Infectious Disease Transmission, Vertical prevention & control, Mice, Molecular Structure, Oocysts drug effects, Pregnancy, Quinolines chemistry, Toxoplasma ultrastructure, Antiprotozoal Agents pharmacology, Carbamates chemistry, Decoquinate pharmacology, Quinolines pharmacology, Toxoplasma drug effects, Toxoplasmosis, Animal drug therapy, Toxoplasmosis, Animal parasitology
- Abstract
The quinolone decoquinate (DCQ) is widely used in veterinary practice for the treatment of bacterial and parasitic infections, most notably, coccidiosis in poultry and in ruminants. We have investigated the effects of treatment of Toxoplasma gondii in infected human foreskin fibroblasts (HFF) with DCQ. This induced distinct alterations in the parasite mitochondrion within 24 h, which persisted even after long-term (500 nM, 52 days) treatment, although there was no parasiticidal effect. Based on the low half-maximal effective concentration (IC
50 ) of 1.1 nM and the high selectivity index of >5000, the efficacy of oral treatment of pregnant mice experimentally infected with T. gondii oocysts with DCQ at 10 mg/kg/day for 5 days was assessed. However, the treatment had detrimental effects, induced higher neonatal mortality than T. gondii infection alone, and did not prevent vertical transmission. Thus, three quinoline- O -carbamate derivatives of DCQ, anticipated to have better physicochemical properties than DCQ, were assessed in vitro. One such compound, RMB060, displayed an exceedingly low IC50 of 0.07 nM, when applied concomitantly with the infection of host cells and had no impact on HFF viability at 10 µM. As was the case for DCQ, RMB060 treatment resulted in the alteration of the mitochondrial matrix and loss of cristae, but the changes became apparent at just 6 h after the commencement of treatment. After 48 h, RMB060 induced the expression of the bradyzoite antigen BAG1, but TEM did not reveal any other features reminiscent of bradyzoites. The exposure of infected cultures to 300 nM RMB060 for 52 days did not result in the complete killing of all tachyzoites, although mitochondria remained ultrastructurally damaged and there was a slower proliferation rate. The treatment of mice infected with T. gondii oocysts with RMB060 did reduce parasite burden in non-pregnant mice and dams, but vertical transmission to pups could not be prevented.- Published
- 2021
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40. One health therapeutics: Target-Based drug development for cryptosporidiosis and other apicomplexa diseases.
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Van Voorhis WC, Hulverson MA, Choi R, Huang W, Arnold SLM, Schaefer DA, Betzer DP, Vidadala RSR, Lee S, Whitman GR, Barrett LK, Maly DJ, Riggs MW, Fan E, Kennedy TJ, Tzipori S, Doggett JS, Winzer P, Anghel N, Imhof D, Müller J, Hemphill A, Ferre I, Sanchez-Sanchez R, Ortega-Mora LM, and Ojo KK
- Subjects
- Animals, Apicomplexa, Humans, Antiparasitic Agents pharmacology, Cryptosporidiosis drug therapy, One Health, Piperidines pharmacology, Pyrimidines pharmacology, Quinolines pharmacology
- Abstract
This is a review of the development of bumped-kinase inhibitors (BKIs) for the therapy of One Health parasitic apicomplexan diseases. Many apicomplexan infections are shared between humans and livestock, such as cryptosporidiosis and toxoplasmosis, as well as livestock only diseases such as neosporosis. We have demonstrated proof-of-concept for BKI therapy in livestock models of cryptosporidiosis (newborn calves infected with Cryptosporidium parvum), toxoplasmosis (pregnant sheep infected with Toxoplasma gondii), and neosporosis (pregnant sheep infected with Neospora caninum). We discuss the potential uses of BKIs for the treatment of diseases caused by apicomplexan parasites in animals and humans, and the improvements that need to be made to further develop BKIs., (Copyright © 2020. Published by Elsevier B.V.)
- Published
- 2021
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41. Evaluation of anti-biofilm and cytotoxic effect of a gel formulation with Pluronic F-127 and silver nanoparticles as a potential treatment for skin wounds.
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Alvarado-Gomez E, Martínez-Castañon G, Sanchez-Sanchez R, Ganem-Rondero A, Yacaman MJ, and Martinez-Gutierrez F
- Subjects
- Biofilms growth & development, Female, Fibroblasts metabolism, Fibroblasts pathology, Humans, Male, Skin metabolism, Skin pathology, Wounds and Injuries metabolism, Wounds and Injuries pathology, Biofilms drug effects, Cytotoxins chemistry, Cytotoxins pharmacology, Hydrogels chemistry, Hydrogels pharmacology, Metal Nanoparticles chemistry, Metal Nanoparticles therapeutic use, Poloxamer chemistry, Poloxamer pharmacology, Pseudomonas aeruginosa physiology, Silver chemistry, Silver pharmacology, Skin injuries, Staphylococcus aureus physiology, Wounds and Injuries drug therapy
- Abstract
The skin wounds cause serious burden to healthcare systems. The lack of sterility of the innate barrier function of the skin facilitates the development of microbial communities within the wound environment especially in biofilm form. Since biofilm is difficult to eradicate, new treatments have been established, such as silver nanoparticles (AgNPs), which antimicrobial and anti-biofilm properties have been studied, nevertheless, their toxic effects are known too. Different concentrations of AgNPs stabilized with a biocompatible and thermo-reversible vehicle as hydrogel Pluronic F-127 were synthesized, those formulations presented interesting thermo-reversibility which could be used to apply on wounds. The formulations (Gel 62.5, 125, and 250 ppm of AgNPs) proposed in this study showed in vitro a total inhibition of clinical strains (Staphylococcus aureus and Pseudomonas aeruginosa) in planktonic form, as well as, anti-biofilm activity was archived with the formulation of Gel 250 ppm, a total inhibition of biofilm formation with mixed culture was registered in the first 30 min of biofilm growth; even more, the viability of human fibroblasts with all gels formulations was >95%, in contrast to silver sulfadiazine cream 1% which showed the highest cytotoxic effect. PF-127 gel with AgNPs could be a prophylactic treatment for skin wounds, because its activity in critical steps on biofilm formation., (Copyright © 2018 Elsevier B.V. All rights reserved.)
- Published
- 2018
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42. Effects of fetal genotype and sex on developmental response to maternal malnutrition.
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Cogollos L, Garcia-Contreras C, Vazquez-Gomez M, Astiz S, Sanchez-Sanchez R, Gomez-Fidalgo E, Ovilo C, Isabel B, and Gonzalez-Bulnes A
- Subjects
- Adiposity, Alleles, Animals, Animals, Inbred Strains, Crosses, Genetic, Female, Fetal Growth Retardation genetics, Fetal Growth Retardation metabolism, Fetal Growth Retardation pathology, Fetus metabolism, Fetus pathology, Heterozygote, Homozygote, Insemination, Artificial veterinary, Male, Pregnancy, Receptors, Leptin genetics, Receptors, Leptin metabolism, Sex Characteristics, Spain, Sus scrofa, Fatty Acids, Essential metabolism, Fetal Development, Fetal Growth Retardation etiology, Malnutrition physiopathology, Maternal Nutritional Physiological Phenomena, Organogenesis
- Abstract
The present study aimed to determine whether developmental patterns, adiposity level and fatty-acid composition of fetuses exposed to maternal malnutrition are driven by their sex or their genotype, or both, as these may modulate the adaptive response to the intrauterine environment independently of the maternal genotype. We used a single maternal genotype (purebred Iberian (IB) sows), which was inseminated with heterospermic semen (obtained by mixing semen from Iberian and Large White (LW) boars), to obtain four different subsets of fetuses (male and female, purebred (IB×IB) and crossbred (IB×LW)) in Iberian purebred sows. Analysis of fetal phenotypes indicated a better adaptive response of the female offspring, which was modulated by their genotype. When faced with prenatal undernutrition, females prioritised the growth of vital organs (brain, liver, lungs, kidneys and intestine) at the expense of bone and muscle. Moreover, the analysis of fat composition showed a higher availability of essential fatty acids in the female sex than in their male counterparts and also in the Iberian genotype than in crossbred fetuses. These results are of high translational value for understanding ethnic differences in prenatal programming of postnatal health and disease status, and show evidence that prenatal development and metabolic traits are primarily determined by fetal sex and strongly modulated by fetal genotype.
- Published
- 2017
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43. Ontogeny of Sex-Related Differences in Foetal Developmental Features, Lipid Availability and Fatty Acid Composition.
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Garcia-Contreras C, Vazquez-Gomez M, Astiz S, Torres-Rovira L, Sanchez-Sanchez R, Gomez-Fidalgo E, Gonzalez J, Isabel B, Rey A, Ovilo C, and Gonzalez-Bulnes A
- Subjects
- Animals, Cholesterol blood, Female, Lipids blood, Liver embryology, Liver metabolism, Male, Maternal-Fetal Exchange, Muscles embryology, Muscles metabolism, Placenta metabolism, Pregnancy, Sex Factors, Swine, Triglycerides blood, Fatty Acids metabolism, Fetal Development, Fetus metabolism, Lipids biosynthesis
- Abstract
Sex-related differences in lipid availability and fatty acid composition during swine foetal development were investigated. Plasma cholesterol and triglyceride concentrations in the mother were strongly related to the adequacy or inadequacy of foetal development and concomitant activation of protective growth in some organs (brain, heart, liver and spleen). Cholesterol and triglyceride availability was similar in male and female offspring, but female foetuses showed evidence of higher placental transfer of essential fatty acids and synthesis of non-essential fatty acids in muscle and liver. These sex-related differences affected primarily the neutral lipid fraction (triglycerides), which may lead to sex-related postnatal differences in energy partitioning. These results illustrate the strong influence of the maternal lipid profile on foetal development and homeorhesis, and they confirm and extend previous reports that female offspring show better adaptive responses to maternal malnutrition than male offspring. These findings may help guide dietary interventions to ensure adequate fatty acid availability for postnatal development., Competing Interests: The authors declare no conflicts of interest. The funding sponsors had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.
- Published
- 2017
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44. Polyphenols and IUGR pregnancies: Maternal hydroxytyrosol supplementation improves prenatal and early-postnatal growth and metabolism of the offspring.
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Vazquez-Gomez M, Garcia-Contreras C, Torres-Rovira L, Pesantez JL, Gonzalez-Añover P, Gomez-Fidalgo E, Sanchez-Sanchez R, Ovilo C, Isabel B, Astiz S, and Gonzalez-Bulnes A
- Subjects
- Animal Feed, Animal Nutritional Physiological Phenomena, Animals, Blood Glucose analysis, Dietary Supplements, Female, Litter Size drug effects, Male, Phenylethyl Alcohol pharmacology, Postnatal Care, Pregnancy, Prenatal Nutritional Physiological Phenomena, Sex Factors, Swine, Weaning, Antioxidants pharmacology, Birth Weight drug effects, Body Composition drug effects, Fetal Growth Retardation pathology, Phenylethyl Alcohol analogs & derivatives, Polyphenols pharmacology
- Abstract
Hydroxytyrosol is a polyphenol with antioxidant, metabolism-regulatory, anti-inflammatory and immuno-modulatory properties. The present study aimed to determine whether supplementing the maternal diet with hydroxytyrosol during pregnancy can improve pre- and early post-natal developmental patterns and metabolic traits of the offspring. Experiment was performed in Iberian sows fed a restricted diet in order to increase the risk of IUGR. Ten sows were treated daily with 1.5 mg of hydroxytyrosol per kg of feed between Day 35 of pregnancy (30% of total gestational period) until delivery whilst 10 animals were left untreated as controls. Number and weight of offspring were assessed at birth, on post-natal Day 15 and at weaning (25 days-old). At weaning, body composition and plasma indexes of glucose and lipids were measured. Treatment with hydroxytyrosol was associated with higher mean birth weight, lower incidence of piglets with low birth weight. Afterwards, during the lactation period, piglets in the treated group showed a higher body-weight than control piglets; such effects were even stronger in the most prolific litters. These results suggest that maternal supplementation with hydroxytyrosol may improve pre- and early post-natal development of offspring in pregnancies at risk of IUGR.
- Published
- 2017
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45. Fetal Sex Modulates Developmental Response to Maternal Malnutrition.
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Gonzalez-Bulnes A, Torres-Rovira L, Astiz S, Ovilo C, Sanchez-Sanchez R, Gomez-Fidalgo E, Perez-Solana M, Martin-Lluch M, Garcia-Contreras C, and Vazquez-Gomez M
- Subjects
- Adiposity, Animals, Body Weight, Disease Models, Animal, Female, Fetus metabolism, Male, Malnutrition pathology, Maternal-Fetal Exchange physiology, Pregnancy, Pregnancy Complications pathology, Prenatal Exposure Delayed Effects etiology, Prenatal Exposure Delayed Effects physiopathology, Sus scrofa, Fetal Development physiology, Malnutrition complications, Malnutrition physiopathology, Pregnancy Complications physiopathology, Sex Characteristics
- Abstract
The incidence of obesity and metabolic diseases is dramatically high in rapidly developing countries. Causes have been related to intrinsic ethnic features with development of a thrifty genotype for adapting to food scarcity, prenatal programming by undernutrition, and postnatal exposure to obesogenic lifestyle. Observational studies in humans and experimental studies in animal models evidence that the adaptive responses of the offspring may be modulated by their sex. In the contemporary context of world globalization, the new question arising is the existence and extent of sex-related differences in developmental and metabolic traits in case of mixed-race. Hence, in the current study, using a swine model, we compared male and female fetuses that were crossbred from mothers with thrifty genotype and fathers without thrifty genotype. Female conceptuses evidence stronger protective strategies for their adequate growth and postnatal survival. In brief, both male and female fetuses developed a brain-sparing effect but female fetuses were still able to maintain the development of other viscerae than the brain (mainly liver, intestine and kidneys) at the expense of carcass development. Furthermore, these morphometric differences were reinforced by differences in nutrient availability (glucose and cholesterol) favoring female fetuses with severe developmental predicament. These findings set the basis for further studies aiming to increase the knowledge on the interaction between genetic and environmental factors in the determination of adult phenotype.
- Published
- 2015
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46. Activating brown adipose tissue through exercise (ACTIBATE) in young adults: Rationale, design and methodology.
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Sanchez-Delgado G, Martinez-Tellez B, Olza J, Aguilera CM, Labayen I, Ortega FB, Chillon P, Fernandez-Reguera C, Alcantara JMA, Martinez-Avila WD, Muñoz-Hernandez V, Acosta FM, Prados-Ruiz J, Amaro-Gahete FJ, Hidalgo-Garcia L, Rodriguez L, Ruiz YA, Ramirez-Navarro A, Muros-de Fuentes MA, García-Rivero Y, Sanchez-Sanchez R, de Dios Beas Jimenez J, de Teresa C, Navarrete S, Lozano R, Brea-Gomez E, Rubio-Lopez J, Ruiz MR, Cano-Nieto A, Llamas-Elvira JM, Jimenez Rios JA, Gil A, and Ruiz JR
- Subjects
- Adolescent, Adult, Body Temperature Regulation, Energy Metabolism, Female, Heart Rate, Humans, Male, Multimodal Imaging, Positron-Emission Tomography, Tomography, X-Ray Computed, Young Adult, Adipose Tissue, Brown metabolism, Exercise physiology
- Abstract
Aims: The energy expenditure capacity of brown adipose tissue (BAT) makes it an attractive target as a therapy against obesity and type 2 diabetes. BAT activators namely catecholamines, natriuretic peptides and certain myokines, are secreted in response to exercise. ACTIBATE will determine the effect of exercise on BAT activity and mass measured by positron emission tomography/computed tomography (PET/CT, primary outcome) in young adults. ACTIBATE will also investigate the physiological consequences of activating BAT (secondary outcomes)., Methods: ACTIBATE will recruit 150 sedentary, healthy, young adults (50% women) aged 18-25 years. Eligible participants will be randomly assigned to a non-exercise group (n ≈ 50) or one of two exercise groups (n=50 each). Participants in the exercise groups will perform aerobic and strength training 3-4 days/week at a heart rate equivalent to 60% of heart rate reserve (HRres), and at 50% of 1 repetition maximum (RM) for the moderate-intensity group, and at 80% of HRres and 70% RM for the vigorous-intensity group. Laboratory measures completed at baseline and 6 months include BAT activity and mass, resting energy expenditure, meal and cold-induced thermogenesis, body temperature regulation and shivering threshold, body composition and cardiovascular disease risk factors. We will also obtain biopsies from abdominal subcutaneous white adipose tissue and skeletal muscle to analyse the expression of genes encoding proteins involved in the thermogenic machinery., Discussion: Findings from ACTIBATE will have significant implications for our understanding of exercise and its protective effects against the development of type 2 diabetes, obesity and related metabolic diseases. ClinicalTrials.gov ID: NCT02365129., (Copyright © 2015 Elsevier Inc. All rights reserved.)
- Published
- 2015
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47. Rosette-forming glioneuronal tumour (RGNT) of the fourth ventricle: a highly aggressive case.
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García Cabezas S, Serrano Blanch R, Sanchez-Sanchez R, and Palacios Eito A
- Subjects
- Adult, Cerebral Ventricle Neoplasms blood supply, Cerebral Ventricle Neoplasms therapy, Combined Modality Therapy, Diagnostic Imaging, Disease Progression, Glioma blood supply, Glioma therapy, Humans, Male, Microvessels pathology, Necrosis, Neoplasm Invasiveness, Neuroma blood supply, Neuroma therapy, Spinal Cord Neoplasms blood supply, Spinal Cord Neoplasms therapy, Treatment Outcome, Young Adult, Cerebral Ventricle Neoplasms pathology, Fourth Ventricle pathology, Glioma pathology, Neuroma pathology, Spinal Cord pathology, Spinal Cord Neoplasms pathology
- Abstract
Background: The rosette-forming glioneuronal tumour (RGNT) is a rarely encountered tumour that has been included as a new entity in the 2007 edition of the "World Health Organization (WHO) Classification of Tumours of the Central Nervous System". We describe a rather unusual case of multifocal cerebellar RGNT, located in the spinal cord and displaying leptomeningeal spread., Clinical Presentation: Twenty-four-year-old male with history of long-lasting headaches. A magnetic resonance scan revealed three heterogeneous lesions located within both cerebellar hemispheres and the left cerebellopontine angle, in addition to a spinal cord lesion at the level of the cervical region, and images of leptomeningeal spread. Interventions were performed in two stages; these involved resection of two cerebellar lesions, with a histopathological diagnosis of RGNT with atypical microvascular proliferation and focal necrosis. Although these tumours appear to be benign, our case debuted in an aggressive form, both from the radiological point of view and with respect to its histopathological characteristics. For this reason, the patient received adjuvant therapy with chemotherapy and radiotherapy., Conclusions: Experience of RGNT is limited. The prognostic significance of the histological findings of vascular proliferation and necrosis is still unknown. The clinical improvement in our patient endorses our decision to perform aggressive treatment.
- Published
- 2015
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48. Elevated GH/IGF-I promotes mammary tumors in high-fat, but not low-fat, fed mice.
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Gahete MD, Córdoba-Chacón J, Lantvit DD, Ortega-Salas R, Sanchez-Sanchez R, Pérez-Jiménez F, López-Miranda J, Swanson SM, Castaño JP, Luque RM, and Kineman RD
- Subjects
- 9,10-Dimethyl-1,2-benzanthracene toxicity, Animals, Breast Neoplasms complications, Breast Neoplasms pathology, Diet, Fat-Restricted, Diet, High-Fat, Female, Humans, Insulin Resistance genetics, Insulin-Like Growth Factor I deficiency, Mammary Neoplasms, Animal chemically induced, Mammary Neoplasms, Animal complications, Mammary Neoplasms, Animal pathology, Mice, Obesity complications, Obesity genetics, Obesity pathology, Abnormalities, Multiple genetics, Breast Neoplasms genetics, Growth Disorders genetics, Growth Hormone genetics, Insulin-Like Growth Factor I genetics, Mammary Neoplasms, Animal genetics
- Abstract
Growth hormone (GH) and/or insulin-like growth factor I (IGF-I) are thought to promote breast cancer based on reports showing circulating IGF-I levels correlate, in epidemiological studies, with breast cancer risk. Also, mouse models with developmental GH/IGF-I deficiency/resistance are less susceptible to genetic- or chemical-induced mammary tumorigenesis. However, given the metabolic properties of GH, medical strategies have been considered to raise GH to improve body composition and metabolic function in elderly and obese patients. Since hyperlipidemia, inflammation, insulin resistance and obesity increase breast cancer risk, elevating GH may serve to exacerbate cancer progression. To better understand the role GH/IGF-I plays in tumor formation, this study used unique mouse models to determine if reducing GH/IGF-I in adults protects against 7,12-dimethylbenz[α]anthracene (DMBA)-induced mammary tumor development, and if moderate elevations in endogenous GH/IGF-I alter DMBA-induced tumorigenesis in mice fed a standard-chow diet or in mice with altered metabolic function due to high-fat feeding. We observed that adult-onset isolated GH-deficient mice, which also have reduced IGF-I levels, were less susceptible to DMBA-treatment. Specifically, fewer adult-onset isolated GH-deficient mice developed mammary tumors compared with GH-replete controls. In contrast, chow-fed mice with elevated endogenous GH/IGF-I (HiGH mice) were not more susceptible to DMBA-treatment. However, high-fat-fed, HiGH mice showed reduced tumor latency and increased tumor incidence compared with diet-matched controls. These results further support a role of GH/IGF-I in regulating mammary tumorigenesis but suggest the ultimate consequences of GH/IGF-I on breast tumor development are dependent on the diet and/or metabolic status., (Published by Oxford University Press 2014.)
- Published
- 2014
- Full Text
- View/download PDF
49. Early-postnatal changes in adiposity and lipids profile by transgenerational developmental programming in swine with obesity/leptin resistance.
- Author
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Gonzalez-Bulnes A, Astiz S, Ovilo C, Lopez-Bote CJ, Sanchez-Sanchez R, Perez-Solana ML, Torres-Rovira L, Ayuso M, and Gonzalez J
- Subjects
- Adiposity genetics, Analysis of Variance, Animal Nutritional Physiological Phenomena, Animals, Animals, Newborn, Drug Resistance, Female, Fetal Development genetics, Fetal Development physiology, Inheritance Patterns, Leptin pharmacology, Liver Diseases genetics, Liver Diseases physiopathology, Male, Malnutrition genetics, Metabolic Syndrome genetics, Metabolic Syndrome physiopathology, Obesity genetics, Overnutrition genetics, Swine, Time Factors, Weaning, Adiposity physiology, Leptin metabolism, Lipids analysis, Malnutrition physiopathology, Obesity physiopathology, Overnutrition physiopathology
- Abstract
Maternal malnutrition during pregnancy, both deficiency and excess, induces changes in the intrauterine environment and the metabolic status of the offspring, playing a key role in the growth, status of fitness/obesity and appearance of metabolic disorders during postnatal life. There is increasing evidence that these effects may not be only limited to the first generation of descendants, the offspring directly exposed to metabolic challenges, but to subsequent generations. This study evaluated, in a swine model of obesity/leptin resistance, the existence and extent of transgenerational developmental programming effects. Pre- and postnatal development, adiposity and metabolic features were assessed in the second generation of piglets, descendant of sows exposed to either undernutrition or overnutrition during pregnancy. The results indicated that these piglets exhibited early-postnatal increases in adiposity and disturbances in lipid profiles compatible with the early prodrome of metabolic syndrome, with liver tissue also displaying evidence of paediatric liver disease. These features indicative of early-life metabolic disorders were more evident in the males that were descended from overfed grandmothers and during the transition from milk to solid feeding. Thus, this study provides evidence supporting transgenerational developmental programming and supports the necessity for the development of strategies for avoiding the current epidemics of childhood overweight and obesity., (© 2014 Society for Endocrinology.)
- Published
- 2014
- Full Text
- View/download PDF
50. Similarities and differences between exome sequences found in a variety of tissues from the same individual.
- Author
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Gómez-Ramos A, Sanchez-Sanchez R, Muhaisen A, Rábano A, Soriano E, and Avila J
- Subjects
- Female, Humans, Male, Organ Specificity, Chromosomes, Human genetics, Exome, Genetic Variation
- Abstract
DNA is the most stable nucleic acid and most important store of genetic information. DNA sequences are conserved in virtually all the cells of a multicellular organism. To analyze the sequences of various individuals with distinct pathological disorders, DNA is routinely isolated from blood, independently of the tissue that is the target of the disease. This approach has proven useful for the identification of familial diseases where mutations are present in parental germinal cells. With the capacity to compare DNA sequences from distinct tissues or cells, present technology can be used to study whether DNA sequences in tissues are invariant. Here we explored the presence of specific SNVs (Single Nucleotide Variations) in various tissues of the same individual. We tested for the presence of tissue-specific exonic SNVs, taking blood exome as a control. We analyzed the chromosomal location of these SNVs. The number of SNVs per chromosome was found not to depend on chromosome length, but mainly on the number of protein-coding genes per chromosome. Although similar but not identical patterns of chromosomal distribution of tissue-specific SNVs were found, clear differences were detected. This observation supports the notion that each tissue has a specific SNV exome signature.
- Published
- 2014
- Full Text
- View/download PDF
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