17 results on '"Shannonhouse, John"'
Search Results
2. PACAP38/mast-cell-specific receptor axis mediates repetitive stress-induced headache in mice
- Author
-
Son, Hyeonwi, Zhang, Yan, Shannonhouse, John, Gomez, Ruben, and Kim, Yu Shin
- Published
- 2024
- Full Text
- View/download PDF
3. Pituitary hormones are specifically expressed in trigeminal sensory neurons and contribute to pain responses in the trigeminal system
- Author
-
Hovhannisyan, Anahit H., Son, Hyeonwi, Mecklenburg, Jennifer, Barba-Escobedo, Priscilla Ann, Tram, Meilinn, Gomez, Ruben, Shannonhouse, John, Zou, Yi, Weldon, Korri, Ruparel, Shivani, Lai, Zhao, Tumanov, Alexei V., Kim, Yu Shin, and Akopian, Armen N.
- Published
- 2021
- Full Text
- View/download PDF
4. Fluoxetine disrupts motivation and GABAergic signaling in adolescent female hamsters
- Author
-
Shannonhouse, John L., DuBois, Dustin W., Fincher, Annette S., Vela, Alejandra M., Henry, Morgan M., Wellman, Paul J., Frye, Gerald D., and Morgan, Caurnel
- Published
- 2016
- Full Text
- View/download PDF
5. Meclizine and Metabotropic Glutamate Receptor Agonists Attenuate Severe Pain and Ca2+ Activity of Primary Sensory Neurons in Chemotherapy-Induced Peripheral Neuropathy.
- Author
-
Shannonhouse, John, Bernabucci, Matteo, Gomez, Ruben, Hyeonwi Son, Yan Zhang, Chih-Hsuan Ai, Hirotake Ishida, and Yu Shin Kim
- Subjects
- *
GLUTAMATE receptors , *SENSORY neurons , *PERIPHERAL neuropathy , *CHEMOTHERAPY complications , *ANTIHISTAMINES - Abstract
Chemotherapy-induced peripheral neuropathy (CIPN) affects; 68% of patients undergoing chemotherapy, causing debilitating neuropathic pain and reducing quality of life. Cisplatin is a commonly used platinum-based chemotherapeutic drug known to cause CIPN, possibly by causing oxidative stress damage to primary sensory neurons. Metabotropic glutamate receptors (mGluRs) are widely hypothesized to be involved in pain processing and pain mitigation. Meclizine is an H1 histamine receptor antagonist known to have neuroprotective effects, including an anti-oxidative effect. Here, we used a mouse model of cisplatin-induced CIPN using male and female mice to test agonists of mGluR8 and Group II mGluR as well as meclizine as interventions to reduce cisplatin-induced pain. We performed behavioral pain tests, and we imaged Ca2+ activity of the large population of dorsal root ganglia (DRG) neurons in vivo. For the latter, we used a genetically-encoded Ca2+ indicator, Pirt-GCaMP3, which enabled us to monitor different drug interventions at the level of the intact DRG neuronal ensemble. We found that CIPN increased spontaneous Ca2+ activity in DRG neurons, increased number of Ca2+ transients, and increased hyper-responses to mechanical, thermal, and chemical stimuli. We found that mechanical and thermal pain caused by CIPN was significantly attenuated by the mGluR8 agonist, (S)23,4-DCPG, the Group II mGluR agonist, LY379268, and the H1 histamine receptor antagonist, meclizine. DRG neuronal Ca2+ activity elevated by CIPN was attenuated by LY379268 and meclizine, but not by (S)23,4-DCPG. Furthermore, meclizine and LY379268 attenuated cisplatin-induced weight loss. These results suggest that Group II mGluR agonist, mGluR8 agonist, and meclizine are promising candidates as new treatment options for CIPN, and studies of their mechanisms are warranted. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
6. cytoNet: Spatiotemporal network analysis of cell communities.
- Author
-
Mahadevan, Arun S., Long, Byron L., Hu, Chenyue W., Ryan, David T., Grandel, Nicolas E., Britton, George L., Bustos, Marisol, Gonzalez Porras, Maria A., Stojkova, Katerina, Ligeralde, Andrew, Son, Hyeonwi, Shannonhouse, John, Robinson, Jacob T., Warmflash, Aryeh, Brey, Eric M., Kim, Yu Shin, and Qutub, Amina A.
- Subjects
CELL analysis ,NEURAL stem cells ,CYTOLOGY ,CELL morphology ,HUMAN stem cells ,CELL populations ,REGENERATIVE medicine ,CANCER stem cells - Abstract
We introduce cytoNet, a cloud-based tool to characterize cell populations from microscopy images. cytoNet quantifies spatial topology and functional relationships in cell communities using principles of network science. Capturing multicellular dynamics through graph features, cytoNet also evaluates the effect of cell-cell interactions on individual cell phenotypes. We demonstrate cytoNet's capabilities in four case studies: 1) characterizing the temporal dynamics of neural progenitor cell communities during neural differentiation, 2) identifying communities of pain-sensing neurons in vivo, 3) capturing the effect of cell community on endothelial cell morphology, and 4) investigating the effect of laminin α4 on perivascular niches in adipose tissue. The analytical framework introduced here can be used to study the dynamics of complex cell communities in a quantitative manner, leading to a deeper understanding of environmental effects on cellular behavior. The versatile, cloud-based format of cytoNet makes the image analysis framework accessible to researchers across domains. Author summary: cytoNet provides an online tool to rapidly characterize relationships between objects within images and videos. To study complex tissue, cell and subcellular topologies, cytoNet integrates vision science with the mathematical technique of graph theory. This allows the method to simultaneously identify environmental effects on single cells and on network topology. cytoNet has versatile use across neuroscience, stem cell biology and regenerative medicine. cytoNet applications described in this study include: (1) characterizing how sensing pain alters neural circuit activity, (2) quantifying how vascular cells respond to neurotrophic stimuli overexpressed in the brain after injury or exercise, (3) delineating features of fat tissue that may confer resistance to obesity and (4) uncovering structure-function relationships of human stem cells as they transform into neurons. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
7. In Vivo Calcium Imaging Visualizes Incision-Induced Primary Afferent Sensitization and Its Amelioration by Capsaicin Pretreatment.
- Author
-
Ishida, Hirotake, Yan Zhang, Gomez, Ruben, Shannonhouse, John, Hyeonwi Son, Banik, Ratan, and Yu Shin Kim
- Subjects
AFFERENT pathways ,DORSAL root ganglia ,CAPSAICIN ,SENSORY neurons ,NERVE fibers - Abstract
Previous studies have shown that infiltration of capsaicin into the surgical site can prevent incision-induced spontaneous pain like behaviors and heat hyperalgesia. In the present study, we aimed to monitor primary sensory neuron Ca21 activity in the intact dorsal root ganglia (DRG) using Pirt-GCaMP3 male and female mice pretreated with capsaicin or vehicle before the plantar incision. Intraplantar injection of capsaicin (0.05%) significantly attenuated spontaneous pain, mechanical, and heat hypersensitivity after plantar incision. The Ca21 response in in vivo DRG and in in situ spinal cord was significantly enhanced in the ipsilateral side compared with contralateral side or naive control. Primary sensory nerve fiber length was significantly decreased in the incision skin area in capsaicin-pretreated animals detected by immunohistochemistry and placental alkaline phosphatase (PLAP) staining. Thus, capsaicin pretreatment attenuates incisional pain by suppressing Ca21 response because of degeneration of primary sensory nerve fibers in the skin. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
8. Sex differences in motivational responses to dietary fat in Syrian hamsters.
- Author
-
Shannonhouse, John L., Grater, Danielle M., York, Daniel, Wellman, Paul J., and Morgan, Caurnel
- Subjects
- *
ANHEDONIA , *DIETARY supplements , *TRANQUILIZING drugs , *ANTIDEPRESSANTS , *MESSENGER RNA ,SEX differences (Biology) - Abstract
Women are more likely than men to exhibit motivational disorders (e.g., anhedonia and anxiety) with limited treatment options, and to overconsume high-fat “comfort foods” to improve motivational disruptions. Unfortunately, neurobiological underpinnings for sex differences in motivational disruptions and their responses to dietary fat are poorly understood. To help bridge these fundamental knowledge gaps, we assessed behavioral and neurobiological responses to dietary fat in a hamster model of female-biased motivational lability. Relative to social housing, social separation reduced hedonic drive in a new behavioral assay, the reward investigational preference (RIP) test. Fluoxetine or desipramine treatment for 21, but not 7, days improved RIP test performance. Pharmacologic specificity in this test was shown by non-responsiveness to diazepam, tracazolate, propranolol, or naltrexone. In the anxiety-related feeding/exploration conflict (AFEC) test, social separation worsened latency to eat highly palatable food under anxiogenic conditions, but not in home cages. Social separation also reduced weight gain, food intake, and adiposity while elevating energy expenditure, assessed by caloric efficiency and indirect calorimetry. Furthermore, chronic high-fat feeding improved anhedonic and anxious responses to separation, particularly in females. In the motivation-influencing nucleus accumbens, females, but not males, exhibited a separation-induced anxiety-related decrease in Creb1 mRNA levels and an anhedonia-related decrease in ΔFosb mRNA levels. Consistent with its antidepressant- and anxiolytic-like effects on behavior, high-fat feeding elevated accumbal Creb1 and ΔFosb mRNA levels in females only. Another accumbal reward marker, Tlr4 mRNA, was elevated in females by high-fat feeding. These results show that social separation of hamsters provides a novel model of sex-dependent comorbid anhedonia, anxiety, and anorexia, and implicate accumbal CREB, ΔFosB, and TLR4. Moreover, the results validate a new assay for chronic antidepressant efficacy. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
9. Aquaporin-11 control of testicular fertility markers in Syrian hamsters.
- Author
-
Shannonhouse, John L., Urbanski, Henryk F., Woo, Shih-Lung, Fong, Li An, Goddard, Scott D., Lucas, William F., Jones, Edward R., Wu, Chaodong, and Morgan, Caurnel
- Subjects
- *
AQUAPORINS , *TESTIS , *MAMMAL fertility , *GOLDEN hamster , *PHOTOPERIODISM , *GENETIC markers , *RODENTS - Abstract
Highlights: [•] Differential display PCR revealed candidate genes for control of fertility in testis during photoperiodic modulation. [•] Multiple linear regression modeling helped identify aquaporin-11 as the best fit among the candidate genes. [•] RNA interference of Aqp11, using small interfering RNA demonstrated Aqp11 tonically induces markers of fertility. [•] The collective results provide the first molecular evidence that Aqp11 regulates fertility. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
10. Female-biased anorexia and anxiety in the Syrian hamster.
- Author
-
Shannonhouse, John L., Fong, Li An, Clossen, Bryan L., Hairgrove, Ross E., York, Daniel C., Walker, Benjamin B., Hercules, Gregory W., Mertesdorf, Lauren M., Patel, Margi, and Morgan, Caurnel
- Subjects
- *
ANOREXIA nervosa , *ANXIETY , *GOLDEN hamster , *COMORBIDITY , *SHORING & underpinning , *SOCIAL isolation , *ADRENOCORTICAL hormones - Abstract
Abstract: Anorexia and anxiety cause significant mortality and disability with female biases and frequent comorbidity after puberty, but the scarcity of suitable animal models impedes understanding of their biological underpinnings. It is reported here that in adult or weanling Syrian hamsters, relative to social housing (SH), social separation (SS) induced anorexia characterized as hypophagia, weight loss, reduced adiposity, and hypermetabolism. Following anorexia, SS increased reluctance to feed, and thigmotaxis, in anxiogenic environments. Importantly, anorexia and anxiety were induced post-puberty with female biases. SS also reduced hypothalamic corticotrophin-releasing factor mRNA and serum corticosteroid levels assessed by RT-PCR and RIA, respectively. Consistent with the view that sex differences in adrenal suppression contributed to female biases in anorexia and anxiety by disinhibiting neuroimmune activity, SS elevated hypothalamic interleukin-6 and toll-like receptor 4 mRNA levels. Although corticosteroids were highest during SH, they were within the physiological range and associated with juvenile-like growth of white adipose, bone, and skeletal muscle. These results suggest that hamsters exhibit plasticity in bioenergetic and emotional phenotypes across puberty without an increase in stress responsiveness. Thus, social separation of hamsters provides a model of sex differences in anorexia and anxiety during adulthood and their pathogeneses during adolescence. [Copyright &y& Elsevier]
- Published
- 2014
- Full Text
- View/download PDF
11. A modified anxious behavior test for hamsters.
- Author
-
Shannonhouse, John L., York, Daniel C., and Morgan, Caurnel
- Subjects
- *
ANXIETY , *APPETITE , *BENZODIAZEPINES , *BETA adrenoceptors , *NORADRENALINE , *LABORATORY mice - Abstract
Highlights: [•] AFEC test is modified for anxious behavior in hamsters. [•] AFEC test distinguishes anxious behavior from appetitive or consummatory behavior. [•] Food deprivation elevates anxious behavior in hamsters without increasing consummatory drive. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
12. Letters
- Author
-
Wolff, Michele and Shannonhouse, John
- Subjects
Computers and office automation industries ,Computers ,Government - Abstract
LETTERS Over the Slump I want to clarify two points raised in Ulric Weil's article, 'NCR Finding Success With Niche Marketing Strategy,' that appeared in the April 1 issue of [...]
- Published
- 1988
13. Na+-dependent inactivation of vascular Na+/Ca2+ exchanger responsible for reduced peripheral blood flow in neuropathic pain model.
- Author
-
Ishida, Hirotake, Yamaguchi, Momoka, Saito, Shin-ya, Furukawa, Takuma, Shannonhouse, John L., Kim, Yu Shin, and Ishikawa, Tomohisa
- Subjects
- *
NEURALGIA , *BLOOD flow , *INTRACELLULAR calcium , *PERIPHERAL nerve injuries , *NERVOUS system injuries , *SCIATIC nerve - Abstract
Reduced skin blood flow has been reported in neuropathic pain patients as well as various peripheral neuropathic pain model animals. We have previously shown that vasodilators, which improves reduced skin blood flow, correlatively alleviate neuropathic pain in chronic constriction injury (CCI) mice, a model of neuropathic pain from peripheral nerve injury. Here, we sought to elucidate the mechanism underlying the reduced skin blood flow in CCI rats. The skin blood flow of the ipsilateral plantar arteries was significantly reduced compared to that of the contralateral ones 4 weeks after loose ligation of the sciatic nerve. The contraction induced by noradrenaline, serotonin, and U46619, a thromboxane receptor agonist, in the isolated ipsilateral plantar arteries was significantly enhanced compared to that in the contralateral ones. KB-R7943, a Na+/Ca2+ exchanger (NCX) inhibitor, shifted the concentration-response curves of noradrenaline to the left in the contralateral arteries but had no effect on the ipsilateral side. There was no significant difference in concentration-response curves of noradrenaline between the ipsilateral and contralateral arteries in the presence of KB-R7943. Amiloride, a non-specific inhibitor of Na+ channels and transporters, comparably shifted concentration-response curves of noradrenaline to the left in both the contralateral and ipsilateral arteries. One hundred nM of noradrenaline induced intracellular Ca2+ elevation in the ipsilateral arteries, which was significantly larger than that induced by 300-nM noradrenaline in the contralateral arteries. These results suggest that reduced peripheral blood flow after nerve injury is due to Na+-dependent inactivation of NCX in the ipsilateral plantar arteries. [Display omitted] [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
14. Elucidation of neuronal activity in mouse models of TMJ injury by in vivo GCaMP Ca 2+ imaging of intact trigeminal ganglion neurons.
- Author
-
Son H, Shannonhouse J, Zhang Y, Gomez R, Chung MK, and Kim YS
- Abstract
Patients with temporomandibular disorders (TMD) typically experience facial pain and discomfort or tenderness in the temporomandibular joint (TMJ), causing disability in daily life. Unfortunately, existing treatments for TMD are not always effective, creating a need for more advanced, mechanism-based therapies. In this study, we used in vivo GCaMP3 Ca
2+ imaging of intact trigeminal ganglia (TG) to characterize functional activity of the TG neurons in vivo , specifically in TMJ animal models. This system allows us to observe neuronal activity in intact anatomical, physiological, and clinical conditions and to assess neuronal function and response to various stimuli. We observed a significant increase in spontaneously and transiently activated neurons responding to mechanical, thermal, and chemical stimuli in the TG of forced mouth open (FMO) mice. An inhibitor of the CGRP receptor significantly attenuated FMO-induced facial hypersensitivity. In addition, we confirmed the attenuating effect of CGRP antagonist on FMO-induced sensitization by in vivo GCaMP3 Ca2+ imaging of intact TG. Our results contribute to unraveling the role and activity of TG neurons in the TMJ pain animal models of TMD, bringing us closer understanding the pathophysiological processes underlying TMD. Our study also illustrates the utility of in vivo GCaMP3 Ca2+ imaging of intact TG for studies aimed at developing more targeted and effective treatments for TMD.- Published
- 2024
- Full Text
- View/download PDF
15. Forced mouth opening induces post-traumatic hyperalgesia and associated peripheral sensitization after temporomandibular joints injury in mice.
- Author
-
Alshanqiti I, Son H, Shannonhouse J, Hu J, Kumari S, Parastooei G, Wang S, Ro JY, Kim YS, and Chung MK
- Abstract
Temporomandibular disorder (TMD) is the most prevalent painful condition in the craniofacial area. The pathophysiology of TMD is not fully understood, and it is necessary to understand pathophysiology underlying painful TMD conditions to develop more effective treatment methods. Recent studies suggested that external or intrinsic trauma to TMJ is associated with chronic TMD in patients. Here, we investigated the effects of the TMJ trauma through forced-mouth opening (FMO) in mice to determine pain behaviors and peripheral sensitization of trigeminal nociceptors. FMO increased mechanical hyperalgesia assessed by von Frey test, spontaneous pain-like behaviors assessed by mouse grimace scale, and anxiety-like behaviors assessed by open-field test. In vivo GCaMP Ca
2+ imaging of intact trigeminal ganglia (TG) showed increased spontaneous Ca2+ activity and mechanical hypersensitivity of TG neurons in the FMO compared to the sham group. Ca2+ responses evoked by cold, heat, and capsaicin stimuli were also increased. FMO-induced hyperalgesia and neuronal hyperactivities were not sex dependent. TG neurons sensitized following FMO were primarily small to medium-sized nociceptive afferents. Consistently, most TMJ afferents in the TG were small-sized peptidergic neurons expressing calcitonin gene-related peptides, whereas nonpeptidergic TMJ afferents were relatively low. FMO-induced intraneural inflammation in the surrounding tissues of the TMJ indicates potentially novel mechanisms of peripheral sensitization following TMJ injury. These results suggest that the TMJ injury leads to persistent post-traumatic hyperalgesia associated with peripheral sensitization of trigeminal nociceptors.- Published
- 2024
- Full Text
- View/download PDF
16. In Vivo Calcium Imaging of Neuronal Ensembles in Networks of Primary Sensory Neurons in Intact Dorsal Root Ganglia.
- Author
-
Shannonhouse J, Gomez R, Son H, Zhang Y, and Kim YS
- Subjects
- Mice, Animals, Action Potentials, Sensory Receptor Cells, Pain, Calcium pharmacology, Ganglia, Spinal
- Abstract
Ca
2+ imaging can be used as a proxy for cellular activity, including action potentials and various signaling mechanisms involving Ca2+ entry into the cytoplasm or the release of intracellular Ca2+ stores. Pirt-GCaMP3-based Ca2+ imaging of primary sensory neurons of the dorsal root ganglion (DRG) in mice offers the advantage of simultaneous measurement of a large number of cells. Up to 1,800 neurons can be monitored, allowing neuronal networks and somatosensory processes to be studied as an ensemble in their normal physiological context at a populational level in vivo. The large number of neurons monitored allows the detection of activity patterns that would be challenging to detect using other methods. Stimuli can be applied to the mouse hindpaw, allowing the direct effects of stimuli on the DRG neuron ensemble to be studied. The number of neurons producing Ca2+ transients as well as the amplitude of Ca2+ transients indicates sensitivity to specific sensory modalities. The diameter of neurons provides evidence of activated fiber types (non-noxious mechano vs. noxious pain fibers, Aβ, Aδ, and C fibers). Neurons expressing specific receptors can be genetically labeled with td-Tomato and specific Cre recombinases together with Pirt-GCaMP. Therefore, Pirt-GCaMP3 Ca2+ imaging of DRG provides a powerful tool and model for the analysis of specific sensory modalities and neuron subtypes acting as an ensemble at the populational level to study pain, itch, touch, and other somatosensory signals.- Published
- 2023
- Full Text
- View/download PDF
17. Meclizine and Metabotropic Glutamate Receptor Agonists Attenuate Severe Pain and Ca 2+ Activity of Primary Sensory Neurons in Chemotherapy-Induced Peripheral Neuropathy.
- Author
-
Shannonhouse J, Bernabucci M, Gomez R, Son H, Zhang Y, Ai CH, Ishida H, and Kim YS
- Abstract
Chemotherapy-induced peripheral neuropathy (CIPN) affects ∼68% of patients undergoing chemotherapy, causing debilitating neuropathic pain and reducing quality of life. Cisplatin is a commonly used platinum-based chemotherapeutic drug known to cause CIPN, possibly by causing oxidative stress damage to primary sensory neurons. Metabotropic glutamate receptors (mGluRs) are widely hypothesized to be involved in pain processing and pain mitigation. Meclizine is an H1 histamine receptor antagonist known to have neuroprotective effects, including an anti-oxidative effect. Here, we used a mouse model of cisplatin-induced CIPN using male and female mice to test agonists of mGluR8 and Group II mGluR as well as meclizine as interventions to reduce cisplatin-induced pain. We performed behavioral pain tests, and we imaged Ca
2+ activity of the large population of dorsal root ganglia (DRG) neurons in vivo For the latter, we used a genetically-encoded Ca2+ indicator, Pirt-GCaMP3, which enabled us to monitor different drug interventions at the level of the intact DRG neuronal ensemble. We found that CIPN increased spontaneous Ca2+ activity in DRG neurons, increased number of Ca2+ transients, and increased hyper-responses to mechanical, thermal, and chemical stimuli. We found that mechanical and thermal pain caused by CIPN was significantly attenuated by the mGluR8 agonist, (S)-3,4-DCPG, the Group II mGluR agonist, LY379268, and the H1 histamine receptor antagonist, meclizine. DRG neuronal Ca2+ activity elevated by CIPN was attenuated by LY379268 and meclizine, but not by (S)-3,4-DCPG. Furthermore, meclizine and LY379268 attenuated cisplatin-induced weight loss. These results suggest that Group II mGluR agonist, mGluR8 agonist, and meclizine are promising candidates as new treatment options for CIPN, and studies of their mechanisms are warranted. SIGNIFICANCE STATEMENT Chemotherapy-induced peripheral neuropathy (CIPN) is a painful condition that affects most chemotherapy patients and persists several months or longer after treatment ends. Research on CIPN mechanism is extensive but has produced only few clinically useful treatments. Using in vivo GCaMP Ca2+ imaging in live animals over 1800 neurons/dorsal root ganglia (DRG) at once, we have characterized the effects of the chemotherapeutic drug, cisplatin and three treatments that decrease CIPN pain. Cisplatin increases sensory neuronal Ca2+ activity and develops various sensitization. Metabotropic glutamate receptor (mGluR) agonist, LY379268 or the H1 histamine receptor antagonist, meclizine decreases cisplatin's effects on neuronal Ca2+ activity and reduces pain hypersensitivity. Our results and experiments provide insights into cellular effects of cisplatin and drugs preventing CIPN pain., (Copyright © 2022 the authors.)- Published
- 2022
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.