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2 results on '"Shiguang Cao"'

1. Signal peptidase 21 suppresses cell proliferation, migration, and invasion via the PTEN-PI3K/Akt signaling pathway in lung adenocarcinoma

Author

Na Zhang, Shiguang Cao, Ruiying Sun, Yibei Wang, Luna Liu, Wei Wang, and Xia Meng

Subjects
SPC21, Lung adenocarcinoma, Akt, PTEN, Medicine, Biology (General), QH301-705.5
Abstract

Background In a previous study, a total of 568 differentially expressed proteins including the signal peptidase SPC21 were identified from lung adenocarcinoma (LUAD) and paired normal lung tissues. In this study, the role of SPC21 in LUAD progression was investigated. Methods The relationships and protein-protein interaction network of proteins differentially expressed between paired LUAD samples and adjacent normal tissues samples were identified via the String and Pajek software, respectively. The expression levels of the hub protein SPC21 were analyzed in 84 LUAD-normal paired tissues via immunohistochemistry. The prognostic value of SPC21 mRNA was investigated in 478 LUAD patients from TCGA and GTEx datasets. siRNAs were used in A549 and NCI-H1299 cells to knockdown SPC21. The SPC21 biological function was evaluated using the CCK-8, EdU, plate colony formation, transwell, wound healing, and adhesion assays. Results Patients with lower SPC21 mRNA levels tended to have worse prognosis (overall survival) than those with higher mRNA levels. SPC21 expression was significantly downregulated in LUAD tumor tissues compared with that in paired normal tissues (P

Published
2022
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2. ADFP promotes cell proliferation in lung adenocarcinoma via Akt phosphorylation

Author

Ping Fang, Shiguang Cao, Xia Meng, Boxuan Liu, Wei Wang, Shuanying Yang, Ruiying Sun, Shanshan Deng, and Na Zhang

Subjects
0301 basic medicine, Lung Neoplasms, proliferation, ADFP, Mice, Nude, Adenocarcinoma of Lung, Real-Time Polymerase Chain Reaction, phosphorylation of Akt, Perilipin-2, Small hairpin RNA, 03 medical and health sciences, Mice, 0302 clinical medicine, In vivo, Cell Line, Tumor, Proliferating Cell Nuclear Antigen, medicine, Animals, Humans, Protein kinase B, Mice, Inbred BALB C, Wound Healing, biology, Cell growth, Chemistry, Cell Biology, Original Articles, lung adenocarcinoma, Flow Cytometry, Epithelium, In vitro, Proliferating cell nuclear antigen, 030104 developmental biology, medicine.anatomical_structure, A549 Cells, 030220 oncology & carcinogenesis, Cancer research, biology.protein, Molecular Medicine, Phosphorylation, Original Article, Female, Proto-Oncogene Proteins c-akt
Abstract

Previously, we identified differentially expressed proteins, including ADFP, between lung adenocarcinoma (LAC) tissue and paired normal bronchioloalveolar epithelium. In this study, we investigated the role of ADFP in LAC. ADFP levels in the serum of patients with lung cancer and benign diseases were measured by enzyme‐linked immunosorbent assays (ELISA). shRNA was used to knock‐down or overexpress ADFP in A549 and NCI‐H1299 cells. The biological function of ADFP and its underlying mechanisms was evaluated in vivo and in vitro. ADFP was highly expressed in the serum of lung cancer patients, especially those with LAC. ADFP promoted cell proliferation and up‐regulated the p‐Akt/Akt ratio in A549 and NCI‐H1299 cells in vitro. Furthermore, in nude mice, ADFP promoted tumour formation with high levels of p‐Akt/Akt, Ki67 and proliferating cell nuclear antigen (PCNA). Similar to the effect of ADFP knock‐down, MK‐2206 (a phosphorylation inhibitor of Akt) reduced A549 and NCI‐H1299 cell proliferation. In ADFP‐overexpressing A549 and NCI‐H1299 cells, proliferation was suppressed by MK‐2206 and returned to the control level. ADFP did not regulate invasion, migration or adhesion in LAC cells. Together, these results suggest that ADFP promotes LAC cell proliferation in vitro and in vivo by increasing Akt phosphorylation level.

Published
2020

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