Search

Your search keyword '"Tsukamoto, Kazuhiro"' showing total 228 results
Start Over Author "Tsukamoto, Kazuhiro" Language english
228 results on '"Tsukamoto, Kazuhiro"'

2. Autophagy-Related Gene ATG7 Polymorphism Could Potentially Serve as a Biomarker of the Progression of Atrophic Gastritis.

Author

Yamaguchi, Naoyuki, Sakaguchi, Takuki, Taira, Miki, Fukuda, Daisuke, Ohnita, Ken, Hirayama, Tatsuro, Yashima, Kazuo, Isomoto, Hajime, and Tsukamoto, Kazuhiro

Subjects
ATROPHIC gastritis, GENETIC polymorphisms, SINGLE nucleotide polymorphisms, BIOMARKERS, HELICOBACTER pylori
Abstract

Cytotoxin-associated gene A (CagA) is an oncoprotein that H. pylori injects into the host's gastric epithelial cells and that induces proinflammatory cytokines, such as interleukin (IL)-18 and IL-1β. As a result, it leads to atrophic gastritis (AG), a precancerous lesion of gastric cancer. On the other hand, host cells degrade CagA using autophagy systems. However, few studies exist about the single nucleotide polymorphisms (SNPs) in MAP1LC3A, MAP1LC3B, ATG4A, ATG4B, ATG4C, ATG7, and ATG13, which belong to the autophagy-related genes concerning AG. This study aimed to detect biomarkers associated with AG. Herein, H. pylori-positive subjects (n = 200) were divided into the AG (n = 94) and non-AG (n = 106) groups. Thirty tag SNPs were selected from the above seven candidate genes. The SNP frequency between the two groups was analyzed. The frequency of the C/T or T/T genotype at rs4683787 of ATG7 was significantly lower in the AG group than in the non-AG group (p = 0.034, odds ratio = 0.535). Based on multivariate analysis, the C/C genotype of rs4684787 and age were independently associated with gastric mucosal atrophy. This finding helps stratify the patients needing timely endoscopic screening or early eradication of H. pylori. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

3. The SNP rs6508974 in AXL is a functional polymorphism and a promising biomarker for gefitinib treatment

Author

Kamikatahira, Megumi, Inamine, Tatsuo, Kawano, Sara, Ohba, Haruna, Obata, Kyohei, Iwanaga, Risako, Fukuda, Minoru, Fukuda, Masaaki, Yamaguchi, Hiroyuki, Hirayama, Tatsuro, Mukae, Hiroshi, and Tsukamoto, Kazuhiro

Subjects
schizophrenia, malignant catatonia, modified electroconvulsive therapy (mECT), anti-N-methyl-D-aspartate (NMDA) receptor antibody encephalitis, respiratory tract diseases
Abstract

Somatic mutations in epidermal growth factor receptor (EGFR) found in lung adenocarcinomas are used as biomarkers for the treatment with EGFR-tyrosine kinase inhibitors, including gefitinib. The bypass tracks with amplification of AXL is one of the mechanisms underlying the resistance to gefitinib. We, therefore, carried out a candidate gene approach method to identify AXL polymorphisms associated with the effectiveness of gefitinib. EGFR mutations were first dentified by mutantenriched PCR-restriction fragment length polymorphism (RFLP), and then 2 tag single nucleotide olymorphisms (SNPs) of AXL were examined by PCR-RFLP in 62 Japanese patients with advanced lung adenocarcinoma and treated with gefitinib in two general hospitals in Nagasaki. Subsequently, the association of EFGR mutations and the AXL polymorphism with the effectiveness of gefitinib was examined in these patients. We next examined the effect of the AXL polymorphism on the expression and function of this gene. It is worthy of note that EGFR mutations and the AXL polymorphism rs6508974 independently contributed to the effectiveness of gefitinib, and the polymorphism was proved to be a possible biomarker for selecting non-responders and responders to gefitinib treatment even in the absence of EGFR mutations. Furthermore, this SNP increased the transcriptional activity of the AXL transcript variant 3, one of the three AXL transcript variants, which to some extent increased the epithelial-mesenchymal transition in cancer cells. Taken together, AXL is one of the genes that determine the effectiveness of gefitinib and a biomarker for selecting non-responders and responders among lung adenocarcinoma patients with no EGFR mutations, suggesting that rs6508974 in AXL might be a functional SNP in lung denocarcinoma., Acta medica Nagasakiensia, 65(3), pp.111−121; 2022

Published
2022

4. High Expression of Dihydropyrimidine Dehydrogenase in Lung Adenocarcinoma is Associated With Mutations in Epidermal Growth Factor Receptor: Implications for the Treatment of Non–Small-Cell Lung Cancer Using 5-Fluorouracil

Author

Mochinaga, Koji, Tsuchiya, Tomoshi, Nagasaki, Toshiya, Arai, Junichi, Tominaga, Tetsuro, Yamasaki, Naoya, Matsumoto, Keitaro, Miyazaki, Takuro, Nanashima, Atsushi, Hayashi, Tomayoshi, Tsukamoto, Kazuhiro, and Nagayasu, Takeshi

Published
2014
Full Text
View/download PDF

5. ATG16L1 and ATG12 Gene Polymorphisms Are Involved in the Progression of Atrophic Gastritis.

Author

Yamaguchi, Naoyuki, Sakaguchi, Takuki, Isomoto, Hajime, Inamine, Tatsuo, Ueda, Haruka, Fukuda, Daisuke, Ohnita, Ken, Kanda, Tsutomu, Kurumi, Hiroki, Matsushima, Kayoko, Hirayama, Tatsuro, Yashima, Kazuo, and Tsukamoto, Kazuhiro

Subjects
ATROPHIC gastritis, GENETIC polymorphisms, HELICOBACTER pylori, STOMACH cancer, SINGLE nucleotide polymorphisms
Abstract

Helicobacter pylori (H. pylori) infection causes a progression to atrophic gastritis and results in gastric cancer. Cytotoxin-associated gene A (CagA), a major virulence factor of H. pylori, is injected into gastric epithelial cells using the type IV secretion system. On the other hand, gastric epithelial cells degrade CagA using an autophagy system, which is strictly regulated by the autophagy-related (ATG) genes. This study aimed to identify SNPs in ATG5, ATG10, ATG12, and ATG16L1 associated with gastric mucosal atrophy (GMA). Here, two-hundred H. pylori-positive participants without gastric cancer were included. The degree of GMA was evaluated via the pepsinogen method. Twenty-five SNPs located in the four candidate genes were selected as tag SNPs. The frequency of each SNP between the GMA and the non-GMA group was evaluated. The rs6431655, rs6431659, and rs4663136 in ATG16L1 and rs26537 in ATG12 were independently associated with GMA. Of these four SNPs, the G/G genotype of rs6431659 in ATG16L1 has the highest odd ratio (Odds ratio = 3.835, 95% confidence intervals = 1.337–1.005, p = 0.008). Further functional analyses and prospective analyses with a larger sample size are required. [ABSTRACT FROM AUTHOR]

Published
2023
Full Text
View/download PDF

8. Echinocandin Resistance in Candida auris Occurs in the Murine Gastrointestinal Tract Due to FKS1 Mutations

Author

Hirayama, Tatsuro, Miyazaki, Taiga, Sumiyoshi, Makoto, Ito, Yuya, Ashizawa, Nobuyuki, Takeda, Kazuaki, Iwanaga, Naoki, Takazono, Takahiro, Yamamoto, Kazuko, Izumikawa, Koichi, Yanagihara, Katsunori, Makimura, Koichi, Tsukamoto, Kazuhiro, Kohno, Shigeru, and Mukae, Hiroshi

Subjects
echinocandins, gastrointestinal tract, Candida auris, yeast, antifungal resistance, FKS1
Abstract

Candida auris is resistant to multiple antifungal agents. This study investigated its antifungal susceptibility and explored FKS1 mutations across the isolates from mice enterically colonized with wild-type C. auris and treated with echinocandin. Resistant C. auris with FKS1 mutations, including S639F, S639Y, D642Y, R1354H, or R1354Y, were isolated and found to be micafungin- and caspofungin-resistant in vivo; however, the MICs of isolates with mutation in R1354 remained below the micafungin breakpoint in vitro., Antimicrobial agents and chemotherapy, 67(4), art. no. e0124322; 2023

Published
2023

12. A phase II study of amrubicin and carboplatin for previously untreated patients with extensive-disease small cell lung cancer

Author

Ikeda, Takaya, Fukuda, Minoru, Nakamura, Yoichi, Kinoshita, Akitoshi, Senju, Hiroaki, Nakano, Hirofumi, Kitazaki, Takeshi, Ogawara, Daiki, Taniguchi, Hirokazu, Motoshima, Kohei, Yamaguchi, Hiroyuki, Nakatomi, Katsumi, Shimada, Midori, Nagashima, Seiji, Tsukamoto, Kazuhiro, Kohno, Shigeru, and Nagasaki Thoracic Oncology Group

Published
2014
Full Text
View/download PDF

17. Genetic polymorphisms in CTLA4 and SLC4A2 are differentially associated with the pathogenesis of primary biliary cirrhosis in Japanese patients

Author

Aiba, Yoshihiro, Nakamura, Minoru, Joshita, Satoru, Inamine, Tatsuo, Komori, Atsumasa, Yoshizawa, Kaname, Umemura, Takeji, Horie, Hitomi, Migita, Kiyoshi, Yatsuhashi, Hiroshi, Nakamuta, Makoto, Fukushima, Nobuyoshi, Saoshiro, Takeo, Hayashi, Shigeki, Kouno, Hiroshi, Ota, Hajime, Muro, Toyokichi, Watanabe, Yukio, Nakamura, Yoko, Komeda, Toshiki, Shimada, Masaaki, Masaki, Naohiko, Komatsu, Tatsuji, Yagura, Michiyasu, Sugi, Kazuhiro, Koga, Michiaki, Tsukamoto, Kazuhiro, Tanaka, Eiji, Ishibashi, Hiromi, and The PBC Study Group in NHOSLJ

Published
2011
Full Text
View/download PDF

18. A polymorphism in the integrin αV subunit gene affects the progression of primary biliary cirrhosis in Japanese patients

Author

Inamine, Tatsuo, Nakamura, Minoru, Kawauchi, Ayumi, Shirakawa, Yayoi, Hashiguchi, Hisae, Aiba, Yoshihiro, Taketomi, Akinobu, Shirabe, Ken, Nakamuta, Makoto, Hayashi, Shigeki, Saoshiro, Takeo, Komori, Atsumasa, Yatsuhashi, Hiroshi, Kondo, Shinji, Omagari, Katsuhisa, Maehara, Yoshihiko, Ishibashi, Hiromi, Tsukamoto, Kazuhiro, and the PBC Study Group in NHOSLJ

Published
2011
Full Text
View/download PDF

22. SNP rs2920280 in PSCA Is Associated with Susceptibility to Gastric Mucosal Atrophy and Is a Promising Biomarker in Japanese Individuals with Helicobacter pylori Infection.

Author

Isomoto, Hajime, Sakaguchi, Takuki, Inamine, Tatsuo, Takeshita, Shintaro, Fukuda, Daisuke, Ohnita, Ken, Kanda, Tsutomu, Matsushima, Kayoko, Honda, Tetsuro, Sugihara, Takaaki, Hirayama, Tatsuro, Nakao, Kazuhiko, and Tsukamoto, Kazuhiro

Subjects
HELICOBACTER pylori infections, JAPANESE people, BIOMARKERS, SINGLE nucleotide polymorphisms, GENETIC testing
Abstract

Helicobacter pylori infection results in gastric cancer (GC) with gastric mucosal atrophy (GMA). Some single-nucleotide polymorphisms (SNPs) in the prostate stem cell antigen gene (PSCA) are associated with GC and duodenal ulcers. However, the relationship of other identified SNPs in PSCA with these diseases remains unclear. Herein, the association between PSCA SNPs and GMA among 195 Japanese individuals with H. pylori infection was evaluated. The definition of GMA or non-GMA was based on serum pepsinogen levels or endoscopic findings. Five tag PSCA SNPs were analyzed using PCR high-resolution melting curve analysis with nonlabelled probes. The frequencies of alleles and the genotypes of each tag SNP were compared between the GMA and non-GMA groups. Subsequently, a genetic test was performed using associated SNPs as biomarkers to detect patients developing GMA. Two tag PSCA SNPs (rs2920280 and rs2294008) were related to GMA susceptibility. Individuals with the rs2920280 G/G genotype or the rs2294008 T/T genotype in PSCA had 3.5- and 2.1-fold susceptibility to GMA, respectively. In conclusion, SNP rs2920280 is a possible biomarker for detecting individuals developing GMA. PSCA polymorphisms may be useful biomarkers for predicting GMA linked to GC risk and a screening endoscopy strategy to detect GC related to early stage H. pylori associated GMA. [ABSTRACT FROM AUTHOR]

Published
2022
Full Text
View/download PDF

44. Isolation and characterization of fetal nucleated red blood cells from maternal blood as a target for single cell sequencing‐based non‐invasive genetic testing.

Author

Ito, Noriko, Tsukamoto, Kazuhiro, Taniguchi, Kosuke, Takahashi, Ken, Okamoto, Aikou, Aoki, Hiroaki, Otera‐Takahashi, Yuka, Kitagawa, Michihiro, Ogata‐Kawata, Hiroko, Morita, Hideaki, Hata, Kenichiro, and Nakabayashi, Kazuhiko

Subjects
*ERYTHROCYTES, *Y chromosome, *GENETIC testing, *CELL-free DNA, *GENE libraries, *NUCLEOTIDE sequencing
Abstract

Purpose: Although non‐invasive prenatal testing (NIPT) based on cell‐free DNA (cfDNA) in maternal plasma has been prevailing worldwide, low levels of fetal DNA fraction may lead to false‐negative results. Since fetal cells in maternal blood provide a pure source of fetal genomic DNA, we aimed to establish a workflow to isolate and sequence fetal nucleated red blood cells (fNRBCs) individually as a target for NIPT. Methods: Using male‐bearing pregnancy cases, we isolated fNRBCs individually from maternal blood by FACS, and obtained their genomic sequence data through PCR screening with a Y‐chromosome marker and whole‐genome amplification (WGA)‐based whole‐genome sequencing. Results: The PCR and WGA efficiencies of fNRBC candidates were consistently lower than those of control cells. Sequencing data analyses revealed that although the majority of the fNRBC candidates were confirmed to be of fetal origin, many of the WGA‐based genomic libraries from fNRBCs were considered to have been amplified from a portion of genomic DNA. Conclusions: We established a workflow to isolate and sequence fNRBCs individually. However, our results demonstrated that, to make cell‐based NIPT targeting fNRBCs feasible, cell isolation procedures need to be further refined such that the nuclei of fNRBCs are kept intact. [ABSTRACT FROM AUTHOR]

Published
2021
Full Text
View/download PDF

45. 長崎大学医学部

Author

Fukae, Atsuki, Ikeda, Satoshi, Inoue, Mariko, Tachibana, Toshiko, Inamine, Tatsuo, Kondo, Shinji, Miyahara, Yoshiyuki, Maemura, Koji, Kohno, Shigeru, and Tsukamoto, Kazuhiro

Subjects
liver X receptor gene, dyscholesteremia, single nucleotide polymorphism, coronary artery disease
Abstract

Coronary artery disease (CAD) is a multifactorial and polygenic disorder, which arises due to atherosclerosis of the coronary arteries. Both numerous genetic factors and environmental risk factors may contribute to the pathogenesis of atherosclerosis. Thus, in order to identify the genetic determinants of CAD, an association of genetic polymorphisms of the liver X receptoralpha (NR1H3) and -beta (NR1H2) genes with susceptibility to CAD was examined in a Japanese population. Eight tag single nucleotide polymorphisms (SNPs) in NR1H3 and NR1H2 were analyzed by PCR-restriction fragment length polymorphism or PCR-direct DNA sequencing method in 143 Japanese patients with CAD and 164 healthy control subjects with normal coronary arteries. Subsequently, haplotypes composed of the two tag SNPs in NR1H2 were constructed. Significant differences in the clinical risk factors, dyscholesteremia and diabetes mellitus, were observed between CAD patients and controls (P = 0.040 and P = 0.005, respectively). The frequencies of a C allele in the multiplicative model and its homozygous C/C genotype in the recessive model at rs2279238 in NR1H3 were significantly higher in CAD patients as compared to those in controls (P = 0.039 and P = 0.016, respectively). Furthermore, the frequency of a Hap 4/any diplotype of NR1H2 was significantly higher in CAD patients in comparison to controls (P =, Acta Medica Nagasakiensia, 55(2), pp.69-76; 2011

Published
2011

46. JAK/STAT3 and NF-κB Signaling Pathways Regulate Cancer Stem-Cell Properties in Anaplastic Thyroid Cancer Cells.

Author

Shiraiwa, Ken, Matsuse, Michiko, Nakazawa, Yuka, Ogi, Tomoo, Suzuki, Keiji, Saenko, Vladimir, Xu, Shuhang, Umezawa, Kazuo, Yamashita, Shunichi, Tsukamoto, Kazuhiro, and Mitsutake, Norisato

Subjects
ANAPLASTIC thyroid cancer, CANCER cells, CANCER stem cells, SMALL interfering RNA, ALDEHYDE dehydrogenase, AGAR, IODINE isotopes
Abstract

Background: Anaplastic thyroid carcinoma (ATC) is one of the most aggressive and refractory cancers, and a therapy with a new concept needs to be developed. Recently, research on cancer stem cells (CSCs) has progressed, and CSCs have been suggested to be responsible for metastasis, recurrence, and therapy resistance. In ATC-CSCs, aldehyde dehydrogenase (ALDH) activity is the most reliable marker to enrich CSCs. However, it is just a marker and is not involved in CSC properties. The present study therefore aimed to identify key signaling pathways specific for ATC-CSCs. Methods: A small interfering RNA library targeting 719 kinases was used in a sphere formation assay and cell survival assay using ATC cell lines to select target molecules specific for CSC properties. The functions of the selected candidates were confirmed by sphere formation, cell survival, soft agar, and nude mice xenograft assays using small compound inhibitors. Results: The study focused on PDGFR, JAK, and PIM, whose small interfering RNAs had a higher inhibitory effect on sphere formation, as well as a lower or no effect on regular cell growth in both FRO and KTC3 cells. Next, inhibitors of PDGFR, JAK, STAT3, PIM and NF-κB were used, and all of them successfully suppressed sphere formation in a dose-dependent manner but not regular cell growth, confirming the screening results. Inhibition of the JAK/STAT3 and NF-κB pathways also reduced anchorage-independent growth in soft agar and tumor growth in nude mice. Conclusions: These results suggest that JAK/STAT3 and NF-κB signals play important roles in ATC-CSCs. Targeting these signaling pathways may be a promising approach to treat ATC. [ABSTRACT FROM AUTHOR]

Published
2019
Full Text
View/download PDF

47. Evaluation of Bronchoalveolar Lavage as a Diagnostic Procedure for Primary Pulmonary B-cell Lymphoma

Author

Nakano, Reiji, Oka, Mikio, Watanabe, Masami, Soda, Hiroshi, Terashi, Kenji, Fukuda, Minoru, Narasaki, Fumihiko, Kawabata, Shigeru, Tsurutani, Junji, Nagashima, Seiji, Tsukamoto, Kazuhiro, Noguchi, Yuji, and Kohno, Shigeru

Subjects
respiratory system, respiratory tract diseases
Abstract

We evaluated retrospectively the role of bronchoalveolar lavage (BAL) in the diagnosis of primary pulmonary B-cell lymphoma in four patients. Histological examination of transbronchial lung biopsy specimens showed nonspecific infiltration of small lymphocytes. Examination of BAL fluid (BALF) samples showed lymphocytosis in all samples with dominant B-cell in two patients and T-cell in the remaining patients. In two patients only, there was a increase in B-cell bearing IgM light-chain or M-protein in BALF samples. our results suggest that the diagnostic value of BAL in primary pulmonary B-cell lymphoma is limited and that new molecular biological techniques should be adapted for analysis of BALF samples., Acta medica Nagasakiensia. 1998, 43(3-4), p.62-66

Published
1998

49. A Phase II Study of S-1 for Previously Untreated Elderly Patients with Advanced Non-Small Cell Lung Cancer.

Author

Kasai, Takashi, Nakamura, Yoichi, Fukuda, Minoru, Kitazaki, Takeshi, Nagashima, Seiji, Takatani, Hiroshi, Nakano, Hirofumi, Nakatomi, Katsumi, Ikeda, Takaya, Yamaguchi, Hiroyuki, Tsukamoto, Kazuhiro, Oka, Mikio, and Kohno, Shigeru

Subjects
CANCER treatment, NON-small-cell lung carcinoma, OLDER patients, FLUOROPYRIMIDINES, CANCER chemotherapy, LIVER function tests, NEUTROPENIA, CANCER, THERAPEUTICS
Abstract

Background: S-1, a novel oral fluoropyrimidine, is active in the treatment of non-small cell lung cancer (NSCLC). However, data on S-1 for elderly patients with NSCLC are insufficient. Methods: Eligibility criteria were no prior chemotherapy, stage IIIB or IV NSCLC, performance status 0-1, age >70 years, and adequate hematological, hepatic, and renal functions. Patients received S-1 (40 mg/m2 twice a day) for 28 consecutive days. This schedule was repeated every 6 weeks. The primary end point was the tumor response rate. Results: Thirty-two patients were enrolled and 31 patients were evaluable for response. The patients' median age was 80 years (range: 71-88). The response rate was 22.6% (95% CI: 11-38). Neutropenia, anemia, thrombocytopenia, febrile neutropenia, and diarrhea of grade ≥3 occurred in 6, 6, 10, 3, and 3%, respectively. Conclusions: In elderly patients with previously untreated advanced NSCLC, S-1 appears to be well tolerated and demonstrates encouraging activity. © 2015 S. Karger AG, Basel [ABSTRACT FROM AUTHOR]

Published
2016
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results