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Background Oral lichen planus (OLP) is a common oral mucosal disease, clinically categorized into erosive OLP (EOLP) and non-erosive OLP (NEOLP) based on symptoms, but its pathogenic mechanism remains unclear. This study aims to explore the relationship between OLP and the oral microbiome.Methods We collected oral mucosal samples from 49 patients and 10 healthy individuals and conducted 16S rRNA and ITS gene sequencing to explore the oral fungal and bacterial communities.Results We observed significantly lower α diversity of fungi in the EOLP group, with Candida being significantly enriched as the main dominant genus. In the NEOLP group, Aspergillaceae were significantly enriched. The EOLP group showed significant enrichment of Aggregatibacter and Lactobacillus, but the relative abundance of Streptococcus was notably lower than in the other two groups. In the NEOLP group, two species including Prevotella intermedia were significantly enriched. The microbial co-occurrence and co-exclusion networks display distinct characteristics across the three groups, with Lactobacillus assuming a significant bridging role in the ELOP group.Conclusions Our study indicates that EOLP and NEOLP experience varying degrees of dysbiosis at both the fungal and bacterial levels. Therefore, the pathogenic mechanisms and interactive relationships of these microbiota associated with OLP merit further in-depth investigation.

Parkinson's disease (PD) is a common neurological degenerative disease in clinical practice. Dysarthria is a common motor symptom, with a high incidence and diverse clinical manifestations. PD patients often ignore their dysarthria symptoms until they reach the decompensation stage, and only a small number of patients receive treatment. This article reviews the pathogenesis, evaluation and treatment of dysarthria in PD, providing theoretical basis for early diagnosis and timely treatment of dysarthria in PD.

To evaluate influence of external environment and material composition on skid-resistance durability of high viscosity modified asphalt (HVMA) mixture, the accelerated abrasion test was selected to simulate decay of skid resistance for asphalt mixture by setting different test conditions. The influence of aggregate gradation, asphalt type, ambient humidity and overload on skid-resistance durability of asphalt mixture was discussed, and the significant effect of selected factors on skid-resistance durability of asphalt mixture was revealed. The results show that the skid-resistance durability of SMA-13 (Stone Mastic Asphalt) mixture is better than OGFC-13(Open Graded Friction Course) and PA-13 (Porous Asphalt) mixture under different conditions, and attenuation rate of different indexes of SMA-13 mixture is 1.6–2.2% and 0.8–2.1% lower than compared with OGFC-13 and PA-13 mixture. The attenuation rate of texture depth for asphalt mixture is closely related to aggregate gradation and asphalt, but the attenuation rate of british pendulum number for asphalt mixture with the same mineral aggregate is only significantly affected by the asphalt. The skid resistance of SMA-13 and PA-13 mixture is improved slightly by addition of high viscosity modified asphalt. The attenuation rate of texture depth of SMA-13 and PA-13 mixture with HVMA is reduced about 0.6–3.0% and 2.2–4.2% than SBS modified asphalt, but the attenuation rate of british pendulum number of above mixtures is increased about 1.2–1.6% and 0.3–1.7%. The skid-resistance durability of asphalt mixture with TPS (TAFPACK-Super) and SINOTPS high viscosity agent is better than HVA (High viscosity additive). The skid-resistance durability of asphalt mixture based on texture depth is improved under wet condition. However, the skid-resistance durability of asphalt mixture for british pendulum number gets worse due to water intrusion. The skid-resistance durability of asphalt mixture is rapidly decreased under overload condition. The influence of overload and SBS modified asphalt on skid-resistance durability of asphalt mixture is higher than ambient humidity and HVMA. This paper will provide foundation for high viscosity modified asphalt applied in asphalt pavement.

The comprehensive utilization of coal gangue is low, and this causes serious environment problems and security risks. The physical and chemical properties of coal gangue were analyzed in this paper, and the estimated value of different chemical components of coal gangue was counted. The physical properties of coal gangue aggregate were analyzed, and performance and its influencing factors of base course mixture with coal gangue aggregate were studied. The influence of coal gangue powder and coal gangue aggregate on performance of asphalt mortar and asphalt mixture was analyzed, and possible influencing factors and function mechanism were discussed. The results showed that heavy metal elements of coal gangue applied into asphalt pavement is not seeped out. The crushing value, water absorption and flat elongated particles content of coal gangue aggregate are generally high, but the compressive strength of base course with coal gangue aggregate is verified to meet the strength requirement. The performance, volume stability and durability of base course with coal gangue aggregate are closely related to aggregate gradation, the type and dosage of cementations materials and aggregate. The high temperature performance, temperature susceptibility and viscosity of asphalt mortar are improved by the addition of different coal gangue powder, and improvement effect of activated coal gangue powder on asphalt mortar performance is better. The low temperature performance and adhesion property of asphalt mortar with chemical activated coal gangue powder are improved. The physical reaction between asphalt and different coal gangue powder is only taken place. The temperature stability of asphalt mixture with coal gangue coarse aggregate has achieved the specification requirement. The water stability and low temperature fracture performance of asphalt mixture with fine aggregate replaced by coal gangue are improved. However, the high temperature performance of above mixture is lower than conventional hot mix asphalt mixture. The research results will provide reference for coal gangue applied into asphalt pavement.

Deep learning methods based on time series prediction are widely applied to solving fluid dynamics problems. However, the application characteristics of Long Short-Term Memory (LSTM) and Gate Recurrent Unit (GRU) in fluid mechanics are lacking in the existing research. Therefore, this paper proposes two flow field prediction models combining the Proper Orthogonal Decomposition (POD) method, referred to as POD-LSTM and POD-GRU. Their performance in flow field prediction is compared with numerical simulation data and experimental data respectively. The numerical simulation was obtained by using OpenFOAM-v9, and the experimental data were obtained from Particle Image Velocimetry (PIV). The results show that LSTM performs excellently in predicting velocity field, while GRU excels in predicting pressure field. On the other hand, under the same computational resources and cost, the training time required by GRU is shorter than that of LSTM. Furthermore, as the number of POD modes increases, the overall deviation of LSTM network predictions from actual flow field values tends to increase. In contrast, GRU networks show the opposite trend. This study provides a detailed description of the performance of POD-LSTM and POD-GRU in fluid mechanics. The proposed prediction models demonstrate strong generalization capabilities, further exploring the potential of deep learning methods in fluid mechanics.

Heated tobacco products (HTPs) are a recent category of tobacco products, with their relative safety compared to cigarette smoking and potential to help smokers to quit being two reasons why regulators may consider their market approval. Designed to heat tobacco rather than to burn in order to produce aerosol, different heating techniques are applied to commercial HTPs, which may result in differing aerosol formation. Therefore, each product requires separate assessment. This work focuses on a closed-end HTP (coded as HTP-A), which is electrically heated and designed to allow puffing air flow to bypass its tobacco section, resulting in reduced oxygen concentration within the tobacco section during heating and aerosol forming. To provide a preliminary aerosol chemistry and in vitro toxicological screening, this study assessed HTP-A against a commercial electrically heated HTP (IQOSTM, coded as HTP-B) and a 3R4F reference cigarette. Under Health Canada Intense (HCI) smoking regime, the levels of 9 regulatory priority toxicants in the aerosol of HTP-A were either reduced or comparable to those in HTP-B on a per-stick basis. Additionally, both HTPs showed significant reduction (greater than 90%) in comparison to those measured in mainstream smoke of 3R4F cigarette for these toxicants. Using a set of standard in vitro toxicological assays (Ames, Micronucleus and Neutral Red Uptake), the two HTPs showed no observable responses while significant toxicity responses were recorded for 3R4F’s total particulate matter. Based on these preliminary results, the novel closed-end HTP-A design may provide similar toxicological profiles to the comparator HTP-B. Further toxicological and clinical assessments are warranted to evaluate HTP-A’s potential for exposure or disease risk reduction. [Contrib. Tob. Nicotine Res. 32 (2023) 146–156]

To evaluate the effects of varying proportions of yak meat in feed on the growth of rats and provide a theoretical basis for selecting the optimal feed proportion suitable for rats. This study was designed as a one-variable experiment. Fifty male rats were divided into five groups. The ratios of yak meat to basal feed of rats in four dietary treatment groups were 2:8, 4:6, 6:4, and 8:2, respectively, while those in the control group were only provided a basal diet. In the feeding experiment, the body weights of the rats were recorded on Day 0 and subsequently in the first, second, third, and fourth weeks, along with quantities of feed intake. The body and tail lengths, as well as the waist circumference of the rats, were measured, and blood samples were collected in the fourth week for routine blood and biochemistry investigations. The rats in the 4:6 feed group had the best body condition. They had normal body and tail lengths, smaller waist circumferences, good posture, and were in better overall health than rats in the other groups. The results indicate that the 4:6 diet was optimal for enhancing rats’ growth performance compared to the other diets.

The differences in the chemical composition of Morus nigra (M. nigra) extracts from four different extraction methods, ultrasound-assisted extraction with pure water (WU), pure water decoction extraction (WD), ultrasonic-assisted extraction with formic acid water (FAU), and pure water heat reflux extraction (WHR), were identified using ultra-high performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF-MS/MS) technology.

Cigarette ventilation characteristics are one of the important design factors that affect the combustion state and therefore smoke release. In order to study the changes of ventilation characteristics and the combustion state of cigarettes with different rod circumferences during smoking, a device was designed that could flexibly measure the different ventilation characteristics along the cigarette rod. The device was utilized to measure the changes of the total ventilation rate and filter ventilation rate of cigarettes with different rod circumference in both burned and unburned conditions. At the same time, a test method was implemented to measure the temperature of the combustion coal puff-by-puff during the smoking process. The relationship between ventilation and the combustion state was analyzed on a per-puff basis. The results show that with the decrease of the rod circumference from 24 mm to 20 mm and 17 mm, the total ventilation rate under burning conditions changed considerably compared with unburned conditions, increasing by 55.7%, 60.5% and 74.5% on average, respectively. The ventilation of the cigarette paper played a major role in regulating the ventilation during puffing. With the increase from 17 mm to 24 mm in circumference, the combustion efficiency of the tobacco decreased as indicated by a range of thermophysical parameters of the burning coal.

In order to improve the small intestinal digestibility of rice analogues (RA) and to enhance the nutritional value of the product, rice and potato flour blends were used as raw materials to prepare RA by direct extrusion modification using chemical or biological modifiers combined with high-temperature puffing. The internal structure and nutritional characteristics of RA were analyzed. The results showed that potato starch and amylase enhanced the digestive resistance of RA. The tested chemical modifiers mainly affected the structure and physicochemical properties of starch, in turn increasing the apparent viscosity and decreasing the degree of hydrolysis and digestibility of RA. Octenylsuccinic anhydride (OSA) played a promoting role in the initial stage of intestinal digestion. Propylene oxide (HPPO) reduced the particle size and increased the storage modulus (G’) of RA. Sodium trimetaphosphate (STMP) increased the particle size and G’ of RA. α-Amylase promoted the decomposition of large particles, led to even distribution of proteins on the surface of particles, and inhibited digestion. High-temperature expansion promoted starch decomposition in the early stage of intestinal digestion. Digestion results showed that the combinatorial effect of extrusion modification and high-temperature puffing weakened the film-forming capability of potato starch, which effectively inhibited the decomposition of the starch and weakened the digestive reaction when it just entered the intestine.

The arrival of the big data era, which has been widely used in various industries. In today’s education management mode, in order to improve education development, it is necessary to keep up with the times and reform the education management mode. For this reason, this paper designs an education management model based on the classroom behavior recognition model, which manages students in real time by detecting their classroom behavior status. The classroom behavior recognition model is improved based on the deep learning target detection SSD algorithm. A lightweight model for detecting the behavior of SSD-M3-SFP networks has been designed by replacing the SSD backbone network, VGG, with a MobileNetV3 network after pruning SFPs. Both the speed and accuracy of student behavior recognition can be ensured by the model. Through practical analysis, in the before-and-after analysis of SSD-M3-SFP-based education management model and general education management model, the p-values are 0.001, 0.003, 0.000 on the indicators of the three aspects of education management services, all of which are lower than 0.05, and the difference is significant, and the management effect of SSD-M3-SFP-based education management model is higher than that of general education management model.

Rural tourism, as an indispensable part of rural revitalization, has attracted more and more attention, and deficiencies have been exposed while in the ascendant. During his research on rural tourism service quality, the author develops a rural tourism service quality evaluation scale using the SERVQUAL model and designs a questionnaire accordingly. Q county is selected as the empirical research object of rural tourism service quality, and tourist satisfaction is taken as the entry point to analyze the overall evaluation of rural tourism service quality in Q county so as to carry out factor analysis. Taking rural tourism service quality as the difference between perceived service quality and expected service quality, correlation analysis and regression analysis of the two and tourists’ satisfaction were conducted respectively to explore the relationship between rural tourism service quality and tourists’ satisfaction. The highest score in the comprehensive evaluation of tourists’ satisfaction with rural tourism service quality is reliability (3.49), and the lowest is responsiveness (3.36). The cumulative variance contribution of tangibility, reliability, responsiveness, assurance and empathy was 81.426%. The correlation coefficients of expected tangibles, reliability, responsiveness, assurance and empathy with tourist satisfaction were all less than 0.3, while perceived tangibles, reliability, responsiveness, assurance and empathy were all greater than 0.5. Tourist satisfaction was positively correlated with tangibles, reliability, and responsiveness, but had little correlation with assurance and empathy.

This paper first introduces the lean supply chain international logistics management model and then determines the optimal conditions of the logistics supply chain for B2C enterprises through logistics service integrators and logistics service subcontractors. The paper then employs a combination of the factor analysis method and the factor rotation method to identify 17 key indicators that significantly influence the service quality of the international logistics management model for cross-border e-commerce. It then analyzes these relevant indicators and proposes targeted improvement countermeasures. The results revealed that the respondents have a high level of recognition for the 17 indicators that impact international logistics management, and their mean and standard deviation are stable. The results demonstrate that the first four factors can effectively explain the majority of the original data. Their eigenvalues are 8.157, 2.338, 2.132, and 1.045, and the variance contribution rates of their factors are 47.98%, 13.75%, 12.54%, and 6.14%, respectively, with the cumulative variance contribution rate explaining 80.41% of the original data. By combining the characteristics of the indicators, we identified four key factors: procurement, logistics and customs clearance, after-sale, and overall supply chain links. This study helps to better assist in the development of an international logistics supply chain management model oriented towards cross-border e-commerce.

Objective: This paper discusses the feasibility of using mineral element fingerprinting technology of to identify tobacco producing areas, screens out the effective indicators, and constructs the discrimination model of tobacco producing areas. Methods: The contents of 20 mineral elements in tobacco leaves from 11 producing areas were simultaneously determined by ICP-MS, and the data were analyzed by variance analysis, cluster analysis, principal component analysis and discriminant analysis. Results: The contents of 16 elements were significantly different among producing areas, and principal component analysis resulted in 6 principal components, with the cumulative variance contribution of 89.253%. Using linear discriminant analysis, four elementsmineral, K, Mn, Se and Ba, were screened as the effective indicators to discriminate the geographical origin of tobacco leaves. The established discriminant model could accurately distinguish tobacco leaf samples from different tobacco producing areas. Conclusion: Significant differences were showed in the contents of mineral elements in tobacco leaves from 11 producing areas. Four elements, K, Mn, Se and Ba, are important indicators for distinguishing tobacco producing areas. Mineral element fingerprinting technology can be used for distinguishing tobacco producing areas.

Aim/purpose – Cultural differences play an influential role in driving tourism development and varying outcomes of technology acceptance. The impact of ChatGPT on tourism is yet to be explored. Therefore, this study discussed how ChatGPT may influence tourism development and management in different cultural contexts.

A novel concept is described here that utilizes externally applied heat to a solid rod of reconstituted tobacco biomass to form a stream of aerosol under progressively oxygen-deficient atmosphere. The boundary of auto-ignition was determined at oxygen concentrations of 0%, 5%, 10%, and 21% and then the effects of these different atmospheres on various parameters were studied. Experimental results indicated that the ignition temperature decreased with the increase of oxygen concentration and a negative temperature coefficient (NTC) for atmosphere with oxygen was observed at before but close to ignition temperature. Significant differences in the yields of CO and CO2 between oxygen and oxygen-free atmosphere were observed. The mass of aerosol produced under an inert atmosphere and reduced-oxygen atmosphere were characterized with selected volatile and semi-volatile components, phenols, aldehydes, and other organic compounds of interests. In addition, higher oxygen concentration resulted in higher bulk and surface oxygen content of solid-phase residue, the reduction of carbon and hydrogen element content and related functional groups, and the content of inorganic compounds also exhibited an increase with oxygen concentration. By systematically changing oxygen concentrations of the biomass bed with increasing temperatures, an aerosol stream of controlled chemical composition and yields could be formed without leading to ignition. Key chemical markers of inhalation harm were measured and compared to each other under different degrees close to combustion. Studying reconstituted tobacco or other biomass materials in such a way could provide alternative and useful information in the design heated biomass aerosol generators.

A lit cigarette forms a positive and negative pressure zone by a puff with respect to the position of its paper burn line. Smoke aerosols generated from the two zones are then pulled through the rod under the puff to form the mainstream smoke. This phenomenon is fundamental to the thermophysics and the resultant chemical composition of the mainstream smoke. In this study, we created two different airflow pathways inside a heated tobacco rod by a puff, and investigated the differences in aerosol formation and its chemical compositions. The two different pressure-induced conditions, one through an open-ended tobacco rod (marked as HNB, a label of a designed airflow pathway of commercial heated tobacco products called heat-not-burn prior), and the other through a closed-ended tobacco rod (marked as NSC, a label of a novel-designed airflow pathway of heated tobacco products), were compared for their aerosol collected mass (ACM), the contents of nicotine, water and added aerosol agents such as propylene glycol (PG) and glycerol (VG), as well as selected aldehydes and ketones in the mainstream aerosol. Aerosol particle distribution and the heated temperature along different rod locations were also compared during a puff. The results indicated marked differences in the aerosol formation processes between the two HNB and NSC systems. The transfer ratios of the main aerosol components were significantly higher for the NSC; the levels of formaldehyde and acetaldehyde were significantly lower under the NSC than the HNB condition. There were also significant differences for the aerosol particle number concentration (APNC) and count median diameter (CMD) for the two systems. The lack of convective heat transfer in the aerosol formation under the NSC condition resulted in a relatively stable thermal aerosol generation zone, reflected by the temperature difference between the two systems in the selected locations. The NSC mode of tobacco heating thus offers a novel and enhanced aerosol generation for heat tobacco product designs.

Abstract Background Natural orifice specimen extraction surgery (NOSES) has the advantages of less postoperative pain, fast bowel function recovery, reduced hospital stay and better cosmetic effects. In our centre, anterior resection of rectal cancer with preservation of the left colonic artery (LCA) was performed using NOSES. The feasibility, safety and short-term clinical efficacy of the technique were discussed. Methods A retrospective analysis was performed on 19 patients who underwent laparoscopic anterior resection of rectal cancer with left colonic artery preservation and natural orifice specimen extraction in the Gastrointestinal Surgery Center of Sichuan Cancer Hospital from September 2018 to December 2019. General information about the patients, perioperative data and short-term postoperative results were analysed. Results All operations were completed smoothly, with an average operation duration of 304.36 ± 45.04 min, intraoperative bleeding of 76.31 ± 61.12 ml, first time off bed of 14.42 ± 3.56 h, first time to anus exhaust of 15.26 ± 8.92 h, first time to liquid diet of 2.94 ± 1.12 days, and average postoperative stay of 10.21 ± 3.13 days. Two patients developed temporary intestinal obstruction, and one patient developed pulmonary infection. All of them recovered well after active supportive treatment and were successfully discharged. Conclusion Laparoscopic NOSES for rectal cancer with left colon artery preservation is safe and feasible, with satisfactory short-term results, and is worthy of further clinical investigation.

Objective To discuss the strategy for cervical dumbbell⁃shaped tumors and develop a proposal for individualized therapy. Methods and Results Total 32 patients with cervical dumbbell⁃shaped tumors were recruited from The First Affiliated Hospital of Anhui Medical University from March 2017 to March 2022. All patients received surgical treatments, while the tumors located in C1-2 levels among 7 patients and C2-7 levels among 25 patients. Preoperative McCormick spinal cord function gradeⅠin 9 cases, gradeⅡin 12 cases, grade Ⅲ in 11 cases. Individualized surgical strategies were set according to the results of Toyama typing, preoperative imaging examination and intraoperative spinal stability. Surgery through posterior approach was performed in 26 cases, 2 patients underwent a lateral posterior sternocleidomastoid approach, 2 patients underwent surgery through combined posterior⁃anterolateral approach, and 2 patients received anterior cervical approach. Ninteen out of 32 patients with preoperatively unstable spinal factors or changes in spinal stability resulting from surgery received one⁃stage internal fixation. One patient underwent anterior cervical corpectomy and fusion followed by titanium cage implantation, and 18 patients underwent posterior trans⁃pedicle and lateral mass screw fixation. The results showed a gross total resection for all patients and the success rate was 100% (32/32). The average intraoperative blood loss was about 210 ml. Imaging examinations were performed for all patients within a week after the surgery, the internal fixator were all in good position. One month after the surgery McCormick spinal cord function gradeⅠin 20 cases, gradeⅡin 10 cases, grade Ⅲ in 2 cases. All patients had varying degrees of improvement in symptoms, no cerebrospinal fluid leakage, no serious nervous system infection and other complications, no postoperative cervical kyphosis, and no loosening or breakage of the screws and rods were found in 28 months after surgery, the bone graft fusion and vertebral body shape were good. Conclusions The size of the intervertebral foramen is one of the important factors to be considered in the surgical strategy of cervical dumbbell⁃typed tumors. The surgical management of C1-2 dumbbell⁃typed tumors is different from that of other levels, it is necessary to understand the patient's medical history, symptoms and signs in detail and to improve imaging evaluation before surgery. The development of multiple techniques and typing methods facilitate the development of individualized surgical plans.

In implementing the PMC model, trusting relationships between owners and PMC contractors is the key to resolving the dilemmas caused by institutional constraints and conventional thinking, such as frequent organizational conflicts, low management efficiency, and higher transaction costs. This study utilized PMC projects in Guangxi, Jiangsu, Zhejiang, Tibet, and Guizhou as analysis samples and employed semi-structured field interviews to investigate the antecedents of trust between owners and PMC contractors. In addition, questionnaires were used to quantify the mechanism of trust formation between the two parties and the influence of trust on project performance. The findings indicated that the managerial competence and reputation of the PMC contractor, the adequacy of the owner's authorization, and the effectiveness of the owner's supervisory measures were the key drivers of calculative trust. The adequacy of the owner's authorization, the effectiveness of the owner's supervisory measures, and social similarity play the greatest roles in influencing relational trust. Calculative and relational trust significantly impact project performance, with relational trust having a slightly more substantial impact. This study can serve as a resource for enhancing the performance of PMC projects and achieving PMC project success.

The differences in spatial-temporal distribution patterns and dominant driving factors of vegetation evolution among sub-regions during different historical periods were not clear. Therefore, this study introduced the gravity center model and geodetector to analyze the spatial-temporal change characteristics and dominant driving factors of normalized difference vegetation index (NDVI) in China during 1981–2019 from the perspective of geographic divisions. Results showed that (1) during 1981–2019, the average vegetation coverage in China showed an increasing trend and zones with obviously increasing vegetation NDVI were mainly distributed in the middle reaches of the Yellow River basin and the upper reaches of the Yangtze River. (2) During 1981–2019, the gravity center of national vegetation NDVI was mainly concentrated in Yan’an City and Tongchuan City, showing a southward migration trend as a whole, which indicates that the increment and growth rates of the southern part were greater than those of the northern part. (3) The growth of vegetation in southern China was primarily affected by the temperature, while that of northern China was largely influenced by precipitation. (4) During 1981–2019, the dominant interactive factors of vegetation change for all subregions changed greatly: natural factor (climate or terrain) ∩ other factors → landuse ∩ other factors.

Potato Y virus (PVY) is a bacterial virus that seriously jeopardizes the growth of tobacco. In order to achieve rapid detection of PVY, monoclonal antibodies to PVY-CP protein were prepared and characterized, and colloidal gold immunochromatographic test strips that can be used to detect PVY were established. In this study, we constructed the PVY-CP protein expression plasmid pET28a-PVY-CP, transformed it into Escherichia coli BL21 (DE3) receptor cells to induce the expression of the target protein, and then further purified it as an immunogen to screen hybridoma cells that can stably secrete monoclonal antibody against PVY through cell fusion with hybridoma cells, prepared ascites, and purified the monoclonal antibody by using the caprylic acid saturated ammonium sulfate method. The monoclonal antibody was purified using the method of ammonium octanoate saturated sulfate, labeled with colloidal gold, and the colloidal gold immunochromatographic test strip was established by optimizing the reaction conditions, and the detectability, accuracy and specificity of the test strip were evaluated. The detection limit of the test strip for PVY-CP protein was 1 μg/mL, and there was no cross-reactivity with tobacco bunchy top virus, tomato spotted wilt virus, and tobacco mosaic virus. Comparison of the prepared colloidal gold test strips and the RT-PCR method for actual samples showed that the total conformity rate of the two was 86.67%, and the positive conformity rate was 90%.

Tomato spotted wilt virus (TSWV) is a bacterial virus that seriously harms the growth of tobacco. In order to realize the rapid detection of TSWV, monoclonal antibody to TSWV-NP protein was prepared and characterized, and colloidal gold immunochromatographic test strips were established that can be used to detect TSWV. In this study, the monoclonal antibody to TSWV-NP protein was labeled with colloidal gold, and the reaction conditions were optimized to establish a colloidal gold immunochromatographic test strip assay, and the detectability, accuracy, and specificity of the test strip were evaluated. The detection limit of the test strip for TSWV-NP protein was 0.1 μg/mL, and there was no cross-reactivity with tobacco bunchy top virus, potato Y virus, and tobacco mosaic virus, which had excellent specificity. The results of the colloidal gold test strip and the RT-PCR result method were compared with the actual samples, and the total conformity rate of the two was 95%, and the positive conformity rate was 93.3%.

We present the electronic properties of InAsNBi with N and Bi concentrations based on 16×16 band Hamiltonian model. This model is extended from Band Anti-crossing (BAC) and the dimension of basis states used in the Hamiltonian matrix is 16. Under the condition that the quaternary compound matches the lattice configuration of the InAs substrate, the variations of band gap (Eg), spin-orbit coupling energy (ΔSO) has been examined as a dependency on the mole fractions of N and Bi. Our findings indicate the integration of N and Bi atoms in InAs gives rise to a substantial band gap energy minimization and the growth of spin-orbit coupling energy. Subsequently a crossover (ΔSO = Eg) is observed at a Bi concentration of 0.37%, which contributes to enhancing the effectiveness of photonic devices by suppress Auger recombination. We further reveal the band offset with different N and Bi concentrations and considered the influence of the substrate on the material deviation. The conduction band energy mislignment (ΔEc) of InAsNBi exceeds energy misalignment in the valence bands (ΔEv), which is required to improve thermal-insensitive characteristics of semiconductor devices. Moreover, the significant mastery over the edges of the conduction and valence bands by adjusting the concentration of N and Bi will increase the flexibility to design InAsNBi/InAs structures.

Abstract Background Childbirth preparation plays an important role in reducing maternal mortality and improving women’s childbirth experience. Evaluating childbirth readiness levels before and after interventions provides a basis for formulating more targeted and effective interventions. However, existing tools only assess partial childbirth preparation or have limited evidence of reliability and validity. The aim of this study was thus to develop a new instrument for use during the third trimester to comprehensively assess the readiness level of pregnant women, and test the scale’s psychometric properties. Methods The scale was developed through exploratory mixed methods including qualitative and quantitative phases. A literature review and in-depth semi-structured interviews were utilized to identify the scale items. A Delphi expert consultation evaluated the content validity. Psychometric testing was conducted in a convenience sample of 731 pregnant women in the third trimester (recruited from 3 tertiary hospitals in Hubei province in China). Item analysis was used to screen items; exploratory factor analysis was performed to extract factors; confirmatory factor analysis was performed to evaluate fit on the factor structures. Results The final scale consisted of four dimensions and 18 items that explained 65.8% of the total variance. Confirmative factor analysis (CFA) model showed that the 4-factor model fits the data well. The total Cronbach alpha coefficient of the total scale and 4 factors was 0.935 and 0.853–0.914. The split-half reliability was 0.880. The dimensions comprised “Self-management”, “Information literacy”, “Birth confidence” and “Birth plan”. Conclusions The childbirth readiness scale we developed has good reliability and validity, and can be used to comprehensively assess the readiness level of pregnant women. In addition to understanding the overall level of women’s childbirth readiness, using subscale scores, improvements can be targeted to specific areas of the preparation for childbirth, to improve the efficiency of the intervention.

With the rapid development of global industrialization, the pollution of hexavalent chromium (Cr(Ⅵ)) in soil and groundwater has become increasingly serious. Field investigation and laboratory tests were carried out for the soil polluted by the chromium slag of a ferroalloy plant. The adsorption, infiltration and dispersion experiments were conducted to study the adsorption characteristics and migration mechanism of Cr(Ⅵ) in silty clay. A three-dimensional kinetic mathematical model of Cr(Ⅵ) migration considering convection-dispersion-adsorption was established. The migration and distribution characteristics of Cr(Ⅵ) in groundwater with the pollution source located upstream or downstream of the contaminated site were obtained using the numerical approach. Meanwhile, the effects of dispersity and distribution coefficient) on the spatial and temporal distribution of Cr(Ⅵ) were revealed. The experimental results show that the Langmuir isotherm model well fits the adsorption data of silty clay. The maximum adsorption capacity of silty clay for Cr(Ⅵ) was 466.6 mg/kg. The hydraulic conductivity of silty clay under the infiltration of distilled water and 160 mg/L Cr(Ⅵ) solution was 6.5×10–7–6.7×10–7 cm/s, while it increased to 4.4×10–6 cm/s under infiltration of Cr(Ⅵ) solution with a concentration of 1 000 mg/L. The hydrodynamic dispersion coefficient (D) of silty clay was 1.4×10–4 m2/d. The value of the retardation factor ( ) was found to be 4.2–10. The results of the numerical simulation indicated that when the downstream was contaminated by Cr(Ⅵ), there was still a risk of pollution in the upstream even if molecular diffusion was not considered. The degree of pollution depended on the dispersity of the aquifer. Considering the adsorption of Cr(Ⅵ) by the aquifer, the higher the soil distribution coefficient, the smaller was the distribution range of the Cr(Ⅵ) pollution plume. Therefore, the transformation processes such as Cr(Ⅵ) adsorption should be focused on when predicting the distribution of Cr(Ⅵ) in contaminated sites.

During the active alignment focusing process of car camera assembly, lenses and holders need to be gummed by creamy white and translucent UV glue. The quality of glue dispensing can directly influence the performance of car cameras. Because of the translucency of UV glue, the glue dispensing image may present a low contrast situation, which increases the difficulty of vision detection. This paper proposes a method based on CycleGAN to enhance the glue dispensing image and effectively overcome the problems of blurred and low contrast edges. First, the glue part of the image is segmented into twenty regions. Second, the VGG16 model is used to divide the abovementioned twenty regions into high-contrast images and low-contrast images. Next, the CycleGAN model is trained to enhance the low-contrast images, and then convert them to high-contrast images. Finally, glue contours are extracted by using thresholding segmentation and edge detection to ensure that the quality of glue dispensing can be detected. The success rates of the VGG16 model and the CycleGAN model are 96% and 58%, respectively. The results show that the proposed method can effectively enhance the low contrast part of the glue region and improve the detection accuracy. Specifically, it can increase the gray value difference between the glue and the background from 20 to 55, while the background is substantially retained. The detailed information of the edges of the images is enriched. The accuracy of glue edge extraction can be increased to 99%, which is an approximately 75% improvement compared to the methods without enhancement.

In order to investigate the mechanical properties and failure mechanisms of the rock containing a single hole and double cracks, we used the 3D sand printing technique to prepare the rock-like specimens. Digital image correlation (DIC) method was employed to non-contactly monitor the deformation field of the specimens during the compression process. By calculating the convariance matrix of the horizontal, vertical and shear strains, we introduced the effective variance of the strain field to quantify and identify the failure behaviors of the specimens. The test results indicate that the mechanical properties of the standard 3D sand printed specimens are similar to those of natural sandstones, and the test data show a low dispersion. Therefore, they can be classified as a rock-like material. Due to the inclusion of the cracks, the mechanical properties of the specimens are degraded. The compressive strength and elastic modulus of the specimens each of which contains a single hole and double cracks are reduced by 8.04%-38.91% and 14.44%-27.78%, respectively, compared with those of the specimens each of which merely contains a single hole. Based on the DIC results, three basic types of cracks are identified successfully, i.e. tensile crack (Mode I), shear crack (Mode II) and mixed tensile-shear crack (Mode I-II). All the specimens each of which contains a single hole and double cracks show mixed tensile–shear failure (Mode I-II). The coalescence patterns between the hole and the cracks are influenced by their horizontal distance, and can be classified into tensile coalescence, rotation coalescence and mixed tensile-shear coalescence. The dispersion of the strain field can be quantified by the effective variance of strain field comprehensively. The effective variance of the strain field is close to zero at the initial crack closure stage and the elastic deformation stage. The cracks propagate in different manners after initiation. Based on the effective variance of the strain field, a quantitative method to identify the type of crack was proposed. The cracks can be identified as tensile crack, mixed tensile-shear crack, and shear crack, respectively, when the growth rates of the effective variance fall into the ranges of 0.72×10-2-1.89×10-2, 2.34×10-2-3.59×10-2, and 9.63×10-2-32.40×10-2, respectively.

In the numerical simulation of the macroscopic flow of the concrete, it can optimize the performance indicators of the screw conveyor and improve the uniformity of the material to be discharged in the batch production. The discrete element method is effective. The accuracy of physical parameters of this method is a key issue for the reliability of the simulation results of concrete. In this study, we measured the parameters describing the interaction between gravel, mortar, as well as between these two materials and the wall (steel). The experimentally determined parameters include the particle density, size, shape, coefficient of restitution, coefficients of static, and rolling friction. The cohesion coefficient of mortar particles for batch time was obtained by comparing the spread diameter and flow time in V-funnel experiments and simulation. After these calibration steps, the DEM parameters were validated by comparison of the mass flow rate and driving power by the batch production of screw conveying in simulations and experiments. The calculated results are proved to be close to the experimental data, which demonstrates that the measured DEM parameters are of sufficient accuracy to be used in the simulation of concrete flow performance (mass flow rate, energy consumption) in the screw conveyors.

China leads the world in the development and rollout of 5G network, yet less research has been done on the drivers of Chinese people’s adoption of 5G network, especially the specific role of national sentiments, such as the influence of patriotism on their attitude toward 5G network. The study obtained 804 effective online questionnaires from the respondents of various ages, genders, areas, and educational levels. The results based on the structural equation modeling (SEM) analysis showed that patriotism was an antecedent to Chinese users’ cognition of the conditions provided by the government and telecom operators and the extent that users were influenced by media and surrounding people, which in turn increased users’ expectations of the performance of 5G network and their confidence in adapting to 5G network, and resulted in an increased willingness to use 5G network. This research helps to understand the role of patriotism, which is an emotional factor, in stimulating Chinese users’ attitudes toward 5G network at the time when 5G network is putting into large-scale commercial use in China.

In order to study the influence of joint density on the strength characteristics and failure modes of rock mass, the rock-like specimens with different joint densities were prepared by using 3D sand printing, with the quartz sand and furan resin being employed as the printing materials. The uniaxial compression test was performed on the 3D sand printed specimens, and the digital image correlation (DIC) method was used as a non-contact technique to monitor the full-field deformation. The crack initiation, propagation and coalescence behaviors were quantitatively analyzed from the micromechanics point of view. The results show that the shape of stress-strain curve and the compressive-to-tensile strength ratio of 3D sand printed intact specimen are similar with those of the natural rock, which can be grouped as a rock-like material. The variation processes of stress-strain curves with different joint densities are similar, and can be divided into initial compaction, linear elastic deformation, crack development and residual strength stages. The mechanical properties of specimens decrease with the increase of joint density εf, and the relationship can be expressed as exponential decay functions. By calculating the strain field and displacement vector distribution on the specimen surface, the deformation field distribution and crack propagation of the specimen are found to be closely related to the joint density. The failure mode shifts from axial tension failure (εf = 0.280%) toward mixed failure (εf = 1.193%) and then to tensile coalescence band failure (εf ≥ 1.712%) as the flaw density increases. When the joint density is greater than or equal to 2.739%, the block rotation appears in the tensile coalescence band, and the bookshelf faulting with block rotation is reproduced.

The jointed rock mass exhibits an obviously instantaneous plastic strain under fatigue loading, and thus it is imperative to establish a fatigue constitutive model of jointed rock considering instantaneous plasticity. Based on the assumption that the relationship between the plastic stress of the joint and stress follows a power function, and the accelerated fatigue strain is a second-order nonlinear viscous strain, a joint plastic fatigue component and a double-triggered nonlinear viscous fatigue composite component are proposed. Then a new elastic-plastic viscous fatigue model of jointed rock is established. The results reveal that the proposed model can accurately simulate the fatigue strain of intact rock and jointed rock. Moreover, the proposed model can simulate the stationary fatigue curve of jointed rock mass under fatigue loading, and it can also be applied in the simulation of instantaneous elastic-plastic fatigue, decelerated fatigue, constant speed fatigue and accelerated fatigue of jointed rock under non-stationary fatigue. The model fitting results show that the instantaneous fatigue plastic strain of jointed rock accounts for a large proportion of the instantaneous fatigue strain which should not be ignored. The results of this study are of great reference value in the prediction of fatigue strain and fatigue stability of jointed rock in rock engineering.

Qingqing Wu,1,2 Xiaodong Wang,3 Qian Chen,1,2 Yang Zou,1,2 Xiaoyan Xu,1,2 Tingting Li,1,2 Chen Yu,1,2 Fu Zhu,1,2 Kanyin E Zhang,4 Jingying Jia,1,2 Yanmei Liu1,2 1Center Laboratory, Shanghai Xuhui Central Hospital, Shanghai, People’s Republic of China; 2Shanghai Engineering Research Center of Phase I Clinical Research & Quality Consistency Evaluation for Drugs, Shanghai, People’s Republic of China; 3Changzhou Siyao Pharmaceuticals Co., Ltd, Jiangsu, People’s Republic of China; 4ViaClinical Ltd, Shanghai, People’s Republic of ChinaCorrespondence: Yanmei LiuCenter Laboratory, Shanghai Xuhui Central Hospital, No. 966, Huaihai Road(M), Shanghai, People’s Republic of ChinaTel/Fax +86-21-54030254Email ymliu@shxh-centerlab.comPurpose: To compare the bioequivalence of two formulations of valsartan (80 mg capsules) under fasting and fed conditions in healthy Chinese volunteers using a full-replicate study design.Methods: A total of 78 Subjects were randomly assigned to fasting cohort (n = 48) or fed cohort (n = 30). Each cohort includes 4 single-dose observation periods and 3-day washout periods. Blood samples were collected at designed time point. Plasma concentration of valsartan was analyzed by a validated LC-MS/MS method. Noncompartmental analysis method was employed to determine the pharmacokinetic parameters. Based on the within-subject standard deviation (SWR) of the reference formulation, either reference-scaled average bioequivalence (RSABE) or average bioequivalence (ABE) method was used to evaluate the bioequivalence of the two formulations.Results: Under fasting conditions, the RSABE method was used to evaluate the bioequivalence of Cmax (SWR> 0.294), while ABE method was used to evaluate the bioequivalence of AUC0-t and AUC0-∞. The geometric mean ratio (GMR) of the test/reference for Cmax was 99.52%, and the 95% upper confidence bound was < 0. For AUC0-t and AUC0-∞ comparisons, GMRs were 102.07% and 101.92%, and the 90% CIs of the test/reference were 96.28%– 108.21%, 96.28%– 107.88%, respectively. Under fed conditions, the SWR value of Cmax, AUC0-t and AUC0-∞ all exceeded the cutoff value of 0.294 and therefore, the RSABE method was used. The GMRs for Cmax, AUC0-t and AUC0-∞ were 98.78%, 103.33% and 103.08%, respectively, while the 95% upper confidence bound values were all < 0. These results all met the bioequivalence criteria for highly variable drugs. All adverse events were mild and transient.Conclusion: In this study, the generic formulation of valsartan 80 mg capsule was considered to be bioequivalent to the reference product under both fasting and fed conditions, and satisfied the requirements for marketing in China.NMPA Registration No: CTR20181422.Keywords: valsartan, bioequivalence, replicate, pharmacokinetics, safety

This study was aimed at examining the effects of different deoxidization methods on the physical properties of metallic melts by measuring the changes in the kinematic viscosity, electrical resistivity, surface tension, and density of the metallurgical melts during the heating and cooling processes. Our results indicate that high-temperature physical properties are consistently affected by specific elements and compounds.

Abstract Background This study evaluated the minimal invasiveness, safety, and accuracy of robot-assisted pedicle screw placement procedure using a modified tracer fixation device. Methods Patients were randomly assigned to conventional fixation group (25 patients) and modified fixation group (27 patients). Results No baseline statistical difference was observed between the groups (P > 0.05). The length of unnecessary incision, amount of bleeding, and fixation duration for tracer fixation respectively were 6.08 ± 1.02 mm, 1.46 ± 0.84 ml, and 1.56 ± 0.32 min in the modified fixation group and 40.28 ± 8.52 mm, 12.02 ± 2.24 ml, and 5.08 ± 1.06 min in the conventional group. The difference between both groups was significant (P 0.05). Conclusions The modified minimally invasive procedure for tracer fixation results in minimal trauma and is simple, reliable, and highly safe. Additionally, the procedure does not compromise the accuracy of pedicle screw placement. Thus, it has great clinical applicable value. Trial registration Chinese Clinical Trial Registry: Registration number, ChiCTR1800016680 ; Registration Date, 15/06/2018.

Objectives In view of the operational sea conditions of FPSO (Floating Production Storage and Offloading) ships in the South China Sea, this paper studies the optimization of the multi-point mooring system and side-by-side coupling system of FPSO ships and shuttle tankers. Methods ANSYS-AQWA software and the potential flow theory is used to conduct time-domain analysis, optimize the mooring line segments and proportions, as well as the position of the fairlead; and Fluent software is used to analyze the influence of the change of draft on the wind and current load when the two ships are abreast.Results The results show that a top section of 200 m of steel cable and a bottom section of 316 m of ship chain is the most suitable FPSO multi-point mooring structure for a water depth of 200 m. Maintaining tension on the side-by-side mooring line or increasing its weight can reduce the movement range of the shuttle tanker. After the position of the fairlead is moved to midship, the forces on the fender and mooring line become more uniform. When the weight of the alongside cable is 135 kg/m, the maximum and average values of the fender and alongside cable forces can be minimized.Conclusions The results of this study have reference value for better understanding the influence of mooring parameters on mooring line tension and its influence on the movement of the ship, and can be used to optimize the design of mooring schemes.

C21H22N12O7Hg, monoclinic, P2/n (no. 13), a = 13.655(3) Å, b = 8.7038(17) Å, c = 23.260(5) Å, β = 102.51(3)°, V = 2698.8(10) Å3, Z = 4, Rgt(F) = 0.0446, wRref(F2) = 0.0875, T = 293(2) K.

This article is devoted to the study of Japanese loanwords in Chinese and their classification. Particular attention is paid to the lexical units in writing in Chinese characters, coming from the Japanese language as graphic loanwords in modern Chinese and Japanese, popular on the Chinese-language Internet. The material of the study is loanwords of Japanese origin, selected from dictionaries and scientific works on this topic, as well as word usage in messages on Russian and Chinese Internet forums. We distinguish between two types of Japanese loanwords in Chinese according to how they are borrowed: phonetic and graphic borrowed words. Graphic borrowed from the Japanese language, including the actual Japanese words spelled in Chinese characters, and words created by the Japanese using Chinese characters to convey tokens of other languages, as well as the words of the ancient Chinese language, rethought by the Japanese to create terms, then returned back to modern Chinese language, constitute a characteristic group of graphic loanwords in Chinese.

In recent years, shortage of nurses, high turnover rate, low self-worth, and team instability have become increasingly serious. With the development of positive psychology, more and more nursing managers have begun to attach great importance to the mental health of nurses. Self-esteem, as one of the core indicators of individual mental health, has received extensive attention from researchers in different fields since its inception. This paper reviews and summarizes the research status and development trends of nurses’ self-esteem considering the aspects of self-esteem as a concept, assessment tools, significance, and intervention methods. At the same time, it also puts forward problems that need to be solved by undertaking research into nurses’ self-esteem, in order to provide a reference for further studies on nurses’ self-esteem.