1. [Untitled]
- Author
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Nkere, Chukwuemeka K., Oyekanmi, Joshua O., Silva, Gonçalo, Bömer, Moritz, Atiri, Gabriel I., Onyeka, Joseph, Maroya, Norbert G., Seal, Susan E., and Kumar, P. Lava
- Subjects
0301 basic medicine ,S1 ,Potyvirus ,Loop-mediated isothermal amplification ,Gene Expression ,Diamines ,Biology ,Sensitivity and Specificity ,03 medical and health sciences ,chemistry.chemical_compound ,Virology ,Benzothiazoles ,Organic Chemicals ,Reverse Transcription Loop-mediated Isothermal Amplification ,DNA Primers ,Fluorescent Dyes ,Plant Diseases ,Yam mosaic virus ,Dioscorea ,Chromogenic ,Brief Report ,Reverse Transcription ,General Medicine ,biology.organism_classification ,Reverse transcriptase ,Plant Leaves ,Plant Tubers ,030104 developmental biology ,chemistry ,Quinolines ,SYBR Green I ,Capsid Proteins ,Nucleic Acid Amplification Techniques - Abstract
A closed-tube reverse transcription loop-mediated isothermal amplification (CT-RT-LAMP) assay was developed for the detection of yam mosaic virus (YMV, genus Potyvirus) infecting yam (Dioscorea spp.). The assay uses a set of six oligonucleotide primers targeting the YMV coat protein region, and the amplification products in YMV-positive samples are visualized by chromogenic detection with SYBR Green I dye. The CT-RT-LAMP assay detected YMV in leaf and tuber tissues of infected plants. The assay is 100 times more sensitive in detecting YMV than standard RT-PCR, while maintaining the same specificity. Electronic supplementary material The online version of this article (10.1007/s00705-018-3706-0) contains supplementary material, which is available to authorized users.