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Studies of dairy cow longevity usually focus on the animal life after first calving, with few studies considering early life conditions and their effects on longevity. The objective was to evaluate the effect of birth conditions routinely collected by Dairy Herd Improvement agencies on offspring longevity measured as length of life and length of productive life. Lactanet provided 712,890 records on offspring born in 5,425 Quebec dairy herds between January 1999 and November 2015 for length of life, and 506,066 records on offspring born in 5,089 Quebec dairy herds between January 1999 and December 2013 for length of productive life. Offspring birth conditions used in this study were calving ease (unassisted, pull, surgery, or malpresentation), calf size (small, medium, or large), and twinning (yes or no). Observations were considered censored if the culling reason was "exported," "sold for dairy production," or "rented out" as well as if the animals were not yet culled at the time of data extraction. If offspring were not yet culled when the data were extracted, the last test-day date was considered the censoring date. Conditional inference survival trees were used in this study to analyze the effect of offspring birth conditions on offspring longevity. The hazard ratio of culling between the groups of offspring identified by the survival trees was estimated using a Cox proportional hazard model with herd-year-season as a frailty term. Five offspring groups were identified with different length of life based on their birth condition. Offspring with the highest length of life [median = 3.61 year; median absolute deviation (MAD) = 1.86] were those classified as large or medium birth size and were also the result of an unassisted calving. Small offspring as a result of a twin birth had the lowest length of life (median = 2.20 year; MAD = 1.69) and were 1.52 times more likely to be culled early in life. Six groups were identified with different length of productive life. Offspring that resulted from an unassisted or surgery calving and classified as large or medium when they were born were in the group with the highest length of productive life (median = 2.03 year; MAD = 1.63). Offspring resulting from a malpresentation or pull in a twin birth were in the group with the lowest length of productive life (median = 1.15 year; MAD = 1.11) and were 1.70 times more likely to be culled early in life. In conclusion, birth conditions of calving ease, calf size, and twinning greatly affected offspring longevity, and such information could be used for early selection of replacement candidates.

Achieving high rendering quality in the visualization of large particle data, for example from large-scale molecular dynamics simulations, requires a significant amount of sub-pixel super-sampling, due to very high numbers of particles per pixel. Although it is impossible to super-sample all particles of large-scale data at interactive rates, efficient occlusion culling can decouple the overall data size from a high effective sampling rate of visible particles. However, while the latter is essential for domain scientists to be able to see important data features, performing occlusion culling by sampling or sorting the data is usually slow or error-prone due to visibility estimates of insufficient quality. We present a novel probabilistic culling architecture for super-sampled high-quality rendering of large particle data. Occlusion is dynamically determined at the sub-pixel level, without explicit visibility sorting or data simplification. We introduce confidence maps to probabilistically estimate confidence in the visibility data gathered so far. This enables progressive, confidence-based culling, helping to avoid wrong visibility decisions. In this way, we determine particle visibility with high accuracy, although only a small part of the data set is sampled. This enables extensive super-sampling of (partially) visible particles for high rendering quality, at a fraction of the cost of sampling all particles. For real-time performance with millions of particles, we exploit novel features of recent GPU architectures to group particles into two hierarchy levels, combining fine-grained culling with high frame rates.

The dairy industry is searching for new technologies to address low (50%) estrus detection. However, the lack of information on the potential economic benefits regarding new technology implementation has led some dairy producers to continue using conventional estrus detection methods (e.g. visual observation of standing to be mounted). The objective of this study was to compare the costs of infrared thermography (IRT), visual observation (VO) and ovulation synchronization (Ovsynch: OVS) as breeding strategies at different accuracy levels (Sensitivity [Se], Specificity [Sp]) and pregnancy rates (PR). The costs associated with Breeding, Feeding, Operation Costs, Return to Equity and Culling Risk per estrus detection rate (ER; 30-100%, conception rate for OVS; 30-100%), PR [PR per Parity group; 1-2 (50%), 3-4 (43%), and4 (41%)], and ER accuracy determined the potential financial benefit of each breeding method for a representative farm. Breeding Cost results (Canadian dollars per cow; CAD/cow) showed a higher cost of OVS (138.99) as compared to VO (115.78) and IRT (127.69). Pregnancy Costs were affected by Breeding Cost; however, ER had a significant effect on PR expense for each method, IRT (ER; 30%: 210.38; 100%: 132.19), VO (ER; 30%: 205.93; 100%: 129.39), and OVS (ER; 30%: 247.21; 100%: 155.33). The minimum Se level with a positive Financial Effect for IRT and VO was 60% with a Sp of 100%, and for the OVS was Se 65% and Sp 100%. However, when the Se was 100% a positive Financial Effect was observed with a minimum Sp of 85% for IRT and 75% for VO. Culling Risk was reduced if ER increases differently depending on the parity group. Implementing of IRT as an estrus detection method yields a competitive breeding cost compared to VO and OVS. Further, breeding methods must accomplish at least ∼60% accuracy to have a positive net return.

Feral deer are some of Australia’s worst emerging pest species. Recently, the Government of South Australia launched a four-year program to reduce the populations of feral fallow deer (Dama dama). The program will focus on coordinating landscape-scale aerial culls and seeks to deliver the most efficient and humane approach to aerial culling. We sourced data from a recent program trialling a new approach to aerial culling that incorporated advanced thermal technology and a second shooter with a shotgun to target fallow deer. We reviewed available video and audio records of 104 deer culled in the program to assess efficiency and welfare outcomes. We collected information on the number of shotgun and rifle rounds fired per animal, time between first shot with a shotgun and confirmed death, and pursuit time. We completed field dissections of 20 individuals targeted in the program to assess the lethality of wounds inflicted with shotgun pellets. We also compared program costs and efficiency against published and unpublished data from ten other aerial-culling programs for feral deer in South Australia since 2009. A total of 383 shotgun rounds and 10 rifle rounds were used on 104 fallow deer in the focal program. We documented strong improvements to animal welfare for feral deer targeted with shotguns. The mean (± standard error) time between first shot and confirmed kill with a shotgun was 11.1 ± 0.7 seconds; mean pursuit time between detection and a confirmed kill was 49.5 ± 3.4 seconds. Pursuit time increased with subsequent deer controlled within a group; the maximum pursuit time for any individual was 159.0 seconds. All autopsied animals had received lethal wounds from shotgun pellets, with 100% receiving lung-penetrating damage and 70% also receiving heart-penetrating damage. While a program that uses a shotgun and rifle combined with a second shooter and thermographer can cost more to mobilise, the outcomes measured in cost deer-1 made it the most cost-effective approach of any program we assessed. Control options that deliver improved animal welfare outcomes and increase efficiency are desirable for managing expanding populations of feral deer in South Australia and elsewhere.

Background and Aim: Sow culling is an important practice in commercial swine production because it is directly associated with the economic efficiency of the breeding herd. This study was conducted to analyze the reasons for sow culling and quantify the factors affecting culling in crossbred Landrace and Large White sows under tropical climate. Materials and Methods: A total of 4887 culled sows from one parent stock farm located in Ratchaburi province, Western Thailand, were examined in this study. Culling reasons were grouped into the following eight categories according to farm management: (1) Reproductive disorders, (2) old age, (3) low performance, (4) diseases, (5) lameness, (6) udder problems, (7) body condition, and (8) other illnesses. Logistic regression analysis was used to explore the relationship between culling sows and environmental factors. Effects of parity and season of culling were considered as fixed effects in a statistical model. Results: Descriptive statistics indicated the following factors accounting for sow removals: Old age (34.93%, n=1707), reproductive disorders (29.32%, n=1433), low performance (12.62%, n=617), lameness (12.56%, n=614), diseases (4.8%, n=235), body condition (4.68%, n=229), udder problems (0.79%, n=39), and other illnesses (0.26%, n=13). Parity and season of culling were also found to have a significant effect on sow culling (p

Supernumerary teats (SNT) are a common epidermal abnormality of udders in mammals. The SNT negatively affect machine milking ability, udder health, and animal welfare and sometimes act as reservoirs for undesirable bacteria, resulting in economic losses on calves and lactating cows due to the cost of SNT removal surgery, early culling, and low milk yield. This study aimed to analyze the incidence and genetic parameter of SNT and detect SNT-related genes in Chinese Holstein cattle. In this study, the incidence of SNT was recorded in 4,670 Chinese Holstein cattle (born between 2008 and 2017) from 2 farms, including 734 genotyped cows with 114,485 SNPs. The SNT had a total frequency of 9.8% and estimated heritability of 0.22 (SE = 0.07), which were obtained using a threshold model in the studied Chinese Holstein population. Furthermore, we calculated approximate genetic corre-lations between SNT and the following indicator traits: 12 milk production, 28 body conformation, 5 fertility and reproduction, 5 health, and 9 longevity. Generally, the estimated correlations, such as 305-d milk yield for third parity (-0.55; SE = 0.02) and age at first calving in heifer (0.19; SE = 0.03), were low to moderate. A single-step GWAS was implemented, and 10 genes asso-ciated with SNT located in BTA4 were identified. The region (112.70-112.90 Mb) on BTA4 showed the high -est genetic variance for SNT. The quantitative trait loci on BTA4 was mapped into the RARRES2 gene, which was previously shown to affect adipogenesis and hormone secretion. The WIF1 gene, which was located in BTA5, was also considered as a candidate gene for SNT. Overall, these findings provide useful information for breeders who are interested in reducing SNT.

The culling of injured and non-viable pigs (Sus scrofa) (neonate to breeding stock) is a routine and necessary procedure on most farms. Usually, pigs are culled using one of the following methods: blunt-force trauma (manual and mechanical), captive-bolt stunners, electrical stunning and electrocution or carbon dioxide. Manual blunt-force trauma is one of the most widely used methods due to its low or absent operational and investment costs. However, as a method, it has serious limitations, which include the risk of incomplete concussion, pain, and distress. Manual blunt-force trauma is also aesthetically unpleasant to operators and wider society. To address these issues there has been significant recent research into the development of alternatives to manual blunt-force trauma, these include: captive-bolt stunners, on-farm, gas-based controlled atmosphere systems, low atmospheric pressure systems and electrical stunning. Some of these are currently in commercial use while others are still in the developmental phase. This review brings together the relevant research in this field, evaluating the methods in terms of mechanism of action (mechanical and physiological), effectiveness and animal welfare.

Bovine respiratory disease (BRD) is a common health challenge for dairy calves during the first weeks of life. It can be caused by several environmental factors and pathogens, such as bacteria and viruses, presenting alterations called pulmonary consolidations. BRD shows nonspecific clinical signs, a high rate of subclinical cases and triggers several health consequences, such as impaired growth, reduced weight gain, interference in production and reproduction during the first lactation, and in some cases, animals can also die. In addition economic losses such as: increase in the rate of involuntary culling and reduction of the source of replacement of productive cows in the herd can also occur. There are some forms of diagnosis evaluating clinical signs such as fever and discharges. However, these methods are not sufficient to confirm the clinical diagnosis and do not allow the measurement of the degree of pulmonary lesions. However, the disease can also be diagnosed by pulmonary ultrasound, presenting a high rate of accuracy. This study reviews systematically the main studies that performed pulmonary ultrasound evaluation in the diagnosis of BRD and reported results that showed the efficacy of the use of this method in the clinical diagnosis of the disease. The technique is considered noninvasive, fast, capacity of detecting animals with subclinical BRD and uses the same ultrasound device used in the reproductive management of cows. In addition, ultrasonography has other benefits such as cost reduction, reduced use of antimicrobials, evaluation of the extent of lung lesions, positive influence on animal health and support of profitability of a dairy operation.

Population management of herbivores is widely applied to decrease damage to forests in the northern hemisphere. Culling more antlerless deer, including females and fawns, is an effective means of reducing the population and helps to improve management efficiency. To increase the efficiency of antlerless-biased culling, we assessed the spatiotemporal distribution of the antlerless ratio in culled sika deer (Cervus nippon) in relation to population density. We hypothesized that the antlerless ratio is higher at the center of the distribution than at the margins of the deer’s range, because dispersal processes differ according to sex and age: Young male deer first disperse into an unoccupied area, and then males may become established in the new area for approximately 10–15 years before the number of females increases. A statistical model revealed spatiotemporal changes in the antlerless ratio of culled sika deer. The change in the ratio basically fit the density distribution of the deer, which was estimated independently on the basis of fecal pellet count surveys. The antlerless ratio estimated from the model increased asymptotically as deer density rose. The results support our hypothesis and suggest that antlerless sika deer will be more easily culled at the center rather than at the margins of the distribution range. These findings should help to increase the efficiency of managing the deer via antlerless-biased culling.

Using local resources for ruminant feeding is a way to achieve agroecological production in pastoral farming systems. In North Mediterranean countries, sheep farming systems have evolved towards more intensive systems in lowland and hilly areas, whereas remote and rough pastureland is abandoned and local breeds are rarely maintained; rather, they are progressively replaced by highly productive breeds and their crosses. Using the examples of Corsica (France) and Thessaly (Greece), two dairy sheep farming territories developing intensive farming systems that differ in the livestock breeds they use, we explored the hypothesis that the use of local breeds may not be systematically related to the maintenance of pastoral practices in Mediterranean dairy sheep farming systems. For this purpose, three data sets based on interviews with sheep farmers of local breeds and crossbred flocks were analysed in two study areas. The results demonstrated that local breeds’ adaptive abilities can be used in crossbred flocks or purebred flocks to maintain a feeding system based on pastoral components. However, other drivers also appear to lead into the declining use of local pastoral resources. Apart from the use of local breeds, year-to-year adjustments of replacement and culling rates sometimes have to be applied in order to address the inter-annual variations of the fodder on offer. This paper provides an original approach to studying the link between local breeds and the pastoral components of farming systems by combining synchronic and diachronic analyses of the practices in crossbred and purebred flocks composed of local breeds.

Objectives Since 2012, outbreaks of African swine fever (ASF) in domestic pigs have increased outside of South Africa’s ASF control zone. This study describes the epidemiological investigation and findings of an ASF outbreak in a small-scale pig unit in Gauteng Province and makes recommendations to prevent future outbreaks. Methods PCR testing and molecular analysis were performed on pig tissue samples. Veterinary services conducted epidemiological investigations, forward and backward tracing, and surveillance. Farm management and biosecurity practices were assessed. Quarantine, culling, carcass disposal, and disinfection were implemented. Results ASF virus genotype I was detected. A concurrent ASF outbreak in neighbouring Mpumalanga Province was identified as a possible source. Inadequate biosecurity measures probably facilitated viral transmission. Potential mechanisms for the introduction of the ASF virus include swill feeding practices, free roaming of pigs, scavenging, illegal slaughter, and trade of pig products within the community. Conclusions Molecular typing of the ASF virus linked the outbreak to an ongoing ASF outbreak in Mpumalanga Province. Pig enterprises with poor biosecurity practices may face greater risk of ASF introduction. Small-scale pig keepers should be targeted for ASF awareness and education campaigns. Innovative and cost-effective biosecurity solutions are needed in this resource-poor setting.

Elephant culling is included in National Norms and Standards for the Management of Elephants in the Republic of South Africa, as a last-resort option to reduce elephant population size when require...

The spread of the African swine fever through wild boar population has caused major losses in the pig industry. Therefore, to decrease the population density of wild boar in Poland, the culling of these animals has been dramatically increased. However, the effect of depopulation is unknown because there are no methods that could be used throughout the country to estimate the number of wild boar. Thus, during two hunting seasons an attempt was made to estimate the number of wild boar using data from collective hunts. The forested area of 21 hunting districts (351.5 km2) was divided into five sampling inventory blocks (SIBs), which were used for the statistical analysis of the population density, the harvest rate and results of collective hunts. The average population density obtained by a driving census amounted to 8.19 ± 1.12 and 10.09 ± 1.06 (x̅ ± SE), animals/km2, which indicates that 2879 and 3547 wild boar were living in the study area in 2012/2013 and 2013/2014 seasons respectively. The number of wild boars bagged per one hunting plot was adopted as the harvest success index (HBI). In SIBs the HBI value fluctuated in the range of 0.55 to 1.87 individuals/hunting plot and the population density ranged from 6.46 to 12.18 wild boars/km2. The non-linear regression showed a positive relationship between the HBI index and the population density. The discussion covers the possibility of using collective hunts to estimate the number of wild boar in Poland and in the European Union.

Background Culling is defined as the departure of cows from the herd because of sale, slaughter, salvage, or death. Culling is an important cost for dairy farms. At the same time, culling is a way to increase herd productivity and profitability, as keeping diseased and unproductive cows might result in lower herd milk production and deteriorated reproduction. In order to maximize profitability, the proportion of voluntary culling (selling for dairy purposes or culling due to low production) should be highest among the total culling rate. Previous studies indicate an ascending trend in the proportion of involuntary culling. This observational study used registry data of all cows from herds with ≥5 cow-years in 2016-2017. Objective This study was conducted to assess the reason for culling a dairy cow and its association with age at culling in and around Mekelle using a structured questioner format and direct observation. Visits were performed to each farm to collect data directly from owners or animal attendants and using direct observation. Materials and Methods The cross-sectional study was conducted in and around Mekelle city dairy farm from November, 2016 to April, 2017. Results The most common causes of culling were disease and economic reasons. The most common causes of voluntary culling were economic reasons (17.39), low milk yield (20.29%), and aging (8.70%). The common causes of involuntary culling were diseases (34.78%), injury (7.25%), and infertility (5.80%). In this study rates of culling increased with parity. The highest rate of culling was in animals between parity 2 and 5, increased from (14.49%) to (21.74%) and slightly decreased from parity 6 to 7, i.e. (13.04)% to (8.70)%, respectively. The highest rates of culling were between 3 and 5-years, (17.39)% and (23.19)%, respectively. Conclusion This study indicated animals were culled at premature age because of involuntary culling, which is economically not feasible, diseases were the most common causes of involuntary culling and voluntary culling was the most type of culling in dairy cattle farms and animals mostly culled for decreased production and economic reasons.

The 2015 European Union milk quota abolition initiated considerable expansion in the dairy sector in many European Union countries, most significantly in Ireland. However, this major production increase also had wider societal implications, such as negative environmental and animal welfare consequences. In this article, we used survey data of 441 Irish dairy farmers to assess farmers' attitudes toward the welfare of farmed animals and dairy calves, as well as the reputation of the Irish dairy sector. We also explored how expansion, breeding, calf management, and farmer characteristics relate to calf welfare outcomes (i.e., calf mortality, calf export, and premature culling). In relation to attitudes, farmers expressed a general concern toward animal welfare, while views toward dairy calves and industry reputation were mixed. We used Ward's linkage hierarchical cluster analysis to group farmers based on their attitudes. The cluster analysis revealed 3 distinct groups relating to high, medium, and low animal welfare concern. Herd expansion was negatively associated with being in a higher animal welfare concern cluster, whereas beef trait-focused breeding was positively associated with it. In relation to dairy calf welfare outcomes, our econometric analyses based on multiple regression and binary choice models revealed that expansion was positively associated with calf mortality, whereas improved breeding and calf management factors had a negative association. In addition, being in the high animal welfare concern cluster was negatively associated with calf mortality. Furthermore, breeding decisions were significantly associated with whether calves were exported, and being in the high animal welfare concern cluster was negatively associated with the probability that calves were sent for live export. Finally, farmers' breeding and calf management decisions were associated with premature culling of calves. Overall, this article revealed strategies worth promoting to improve dairy calf welfare, such as beef trait-focused breeding leading to greater dairy-beef integration.

Increasing populations of large herbivores have caused environmental damage around the world, and it is necessary to improve population management strategies. Culling is a traditional management method. Antlerless deer proportions, consisting of adult female deer and fawn in Cervidae in wildlife statistics, are directly related to population increases; thus, the culling-based removal of individuals from habitats and the removal of these antlerless individuals by game hunting and nuisance control might be effective approaches for reducing population sizes. We evaluated the effectiveness of antlerless culling on 17-year density trends in the sika deer (Cervus nippon) population across an area of 1175 km2 in Fukuoka Prefecture (Japan). In 11 out of 47 grids (area measuring 5 by 5 km), the densities of sika deer tended to decline; meanwhile, in the remaining 36 grids, the densities increased. These density trends were explained by changes in the proportion of antlerless culling, as the densities declined with increasing proportions of antlerless deer. The results affirm the theory that antlerless culling is effective in population management; it is posited that antlerless-biased culling could be a crucial measure in managing overabundant populations of herbivores, contributing to more effective conservation of forest environments.

Both influenza C (ICV) and influenza D (IDV) viruses were recently included as bovine respiratory disease (BRD) causes, but their role in BRD has not been evaluated. Therefore, the mortality and reproductive performances of BRD calves with different isolated viruses were determined in this study. Data on 152 BRD calves with bovine viral diarrhoea virus (BVDV), bovine respiratory syncytial virus (BRSV), bovine coronavirus (BCoV), bovine parainfluenza virus 3 (BPIV-3), ICV, or IDV from nasal swab samples using real-time rt-PCR were used. The general data and respiratory signs were recorded immediately, and thereafter, the data on dead or culling calves due to BRD and reproductive performance were collected. The percentages of the BRD calves were 71.7%, 52.6%, 40.8%, 10.5%, 68.4%, and 65.8% for BVDV, BRSV, BCoV, BPIV-3, ICV, and IDV, respectively. Mucous secretion (OR = 4.27) and age ≤ 6 months (OR =14.97) had higher risks of mortality than those with serous secretion and older age. The calves with IDV had lower risks of culling than those without IDV (OR = 0.19). This study shows that most viral infections in BRD calves are a combination of viruses with BVDV, ICV, and IDV. In addition, IDV might have a role in reducing the severity of BRD calves.

The objective of this review is to generate information about the current status of contagious caprine pleuropneumonia (CCPP) which is recognized as highly infectious, and devastating disease of goats for about 140 years. However, current reports indicated that sheep can be affected. CCPP is caused by Mycoplasma capricolum subspecies capripneumoniae (Mccp) which is severe and dramatic mycoplasmas, challenging to isolate and properly identify. Coughing, respiratory distress and very high morbidity and mortality are the main clinical signs that described the disease. Transmission of CCPP occurs through aerosol while animals are in nearby contact. There are a number of approaches for the rapid detection and identification of Mccp based on a PCR. In Africa, Asia and Middle East morbidity and mortality can reach about 100% which causes huge economic loss. Presence of CCPP in Ethiopia reported in 1983 and later confirmed from an outbreak in 1990 in Ogaden, Eastern Ethiopia. Since then CCPP has been considered as endemic disease in Ethiopia. Prevention and control of CCPP is undertaken through vaccination, quarantine, restriction of movement, culling of infected and exposed animals and keeping the hygiene of premises. However, it remains one of the standing problems of goat production. Therefore, more research, awareness creation about CCPP, transmission path way, prevention and control methods, in small ruminant rearing areas to reduce the impact of the disease. Key words: CCPP, goat, Mccp, Ethiopia.

The main trend in the development of dairy farming in the Russian Federation suggests maximising milk yield and reducing milk net cost. The economic effectiveness of industrial dairy farming is largely determined by adequate feeding, as well as effective system of measures to ensure animal health and prevent infectious and non-infectious mass diseases. The main reason for the premature retirement of highly productive cows is based on the factors typical of the intensive technologies used in dairy cattle breeding, which lead to the occurrence of metabolic diseases. It is established that the intensity of metabolism is directly linked to the high productivity of cows. With a highly concentrated, mainly silage-based type of feeding, an imbalance of nutrients is often recorded, in particular as regards the sugar/ protein ratio, leading to deep metabolic disorders and the development of immunodeficiency states. Metabolic disorders in highly productive cows occur as a result of unbalanced diets as far as protein, carbohydrates, vitamins and minerals are concerned. Acidosis, ruminitis and hepatosis are recorded in disordered cows and heifers. The service period exceeds 100 days in 70–75% of cows. Hepatosis and immunodeficiency states are often found in calves born to cows with signs of deep metabolic disorders. Metabolic disorders often remain unnoticed and become apparent only when pronounced pathological changes occur resulting in decreased productivity and ability to reproduce resistant young animals, as well as culling of animals. Metabolic diseases were recorded in 30–70% of cows examined in large dairy farms. The average lifetime productivity of high-yielding cows is (2.1 ± 0.15) lactations in Russia. The results of epidemiological investigations and laboratory testing of sera samples showed that emulsion inactivated vaccines administered to immunodeficient cattle induce higher titres of virus-specific antibodies than those in animals vaccinated with adsorbed vaccines.

Mastitis, the inflammation of the mammary gland, is a major endemic disease affecting dairy production worldwide. Costs of treatment and control of mastitis contributes to major losses to the dairy industry especially if the condition is not promptly and accurately diagnosed, thus necessitating the engagement of regular and reliable means of recognising intra-mammary infections. Somatic cell counting, which has been recognized as a major standard for mastitis diagnosis in milk, was utilized in this study as a direct measure of intramammary inflammation (IMI), along with bacterial culture and isolation to establish the presence of mammary infections in cows from two nomadic herds. Milk from all four quarters (composite samples) of each of 100 cows at various stages of lactation, were obtained and subjected to Levowitz-Weber staining of duplicate smears and direct microscopic counting of somatic cells. Culture and isolation of sterile milk swabs and subsequent identification by morphology, gram staining and biochemical tests were employed to assess the presence of mastitis-causing pathogens in the samples. Using ≤100,000 cells/ml as cut off for non-mastitic milk, sub-clinical mastitis (SM), was determined in 70 (70%) of the examined samples. Contagious pathogens, namely; Staphylococcus aureus, Staphylococcus species and others as well as environmental bacteria, E. coli and Enterococcus species amongst others, were isolated from the samples. This result circuitously indicates the level of losses to dairy production through poor milk quality and yield, costs of treatment and culling obtainable in the nomadic dairying venture. The need for routine evaluation of raw milk and other dairy products emanating from the itinerant pastoralists, using sensitive and reliable parameters to facilitate prompt diagnosis, targeted treatment and rapid control of cow to cow or herd to herd spread of IMI is highlighted in this study.

The association between dry period length (DPL) and time to culling and pregnancy in the subsequent lactation may be important for the economically optimal length of the dry period. Therefore, this study aimed to (1) quantify the association between DPL and hazard of culling and pregnancy in the subsequent lactation; (2) develop continuous functions of DPL for the hazard ratios of culling and pregnancy; and (3) investigate the effect of a cause-specific hazards model and a subdistribution model to analyze competing events. The data used in this observational cohort study were from dairy herd improvement milk test lactation records from 40 states in the United States. After edits, there remained 1,108,515 records from 6,730 herds with the last days dry in 2014 or 2015. The records from 2 adjacent lactations (current, subsequent) were concatenated with the DPL of interest, 21 to 100 d, in between both lactations. We defined 8 DPL categories of 10 d each. Kaplan-Meier survival curves were used to show associations between DPL and time to culling or pregnancy for 3 lactation groups: lactation 1 and 2, lactation 2 and 3, and lactation 3 and greater. To control for confounding factors in Cox proportional models, we included 6 current lactation covariates and 3 time-dependent variables in the survival models. Hazard ratios of culling were estimated for 4 days in milk (DIM) categories from 1 to 450 DIM. Hazard ratios of pregnancy were estimated for 3 DIM categories from 61 to 300 DIM. Competing risk analysis of 8 disposal codes (i.e., farmer reported reasons) for culling and the culling event for pregnancy were conducted by a cause-specific hazards model and a subdistribution model. Hazard ratios were also estimated as quadratic polynomials of DPL. Compared with the reference DPL category of 51 to 60 d, hazard ratios of culling and pregnancy of the other 7 DPL categories ranged between 0.70 and 1.49, and 0.93 and 1.15, respectively. Short DPL were associated with lower risk of culling in the early lactation but not over the entire lactation. Short DPL were associated with greater hazard of pregnancy. Trends in hazard ratios over the ranges of the 8 DPL categories were not always consistent. Competing risk analysis with both models provided little differences in hazard ratios of culling and pregnancy. In conclusion, variations in DPL were associated with meaningful differences in the hazard ratios for culling and pregnancy and minor differences in the relative frequency of disposal codes. Subdistribution hazards models produced hazard ratios similar to cause-specific hazard models. The quadratic polynomials may be useful for decision support on customization of DPL for individual cows.

The camel is one of the most vital domestic animals species for pastoralist livelihood as much as their blood circulation. The annual camel milk production of Ethiopia in 2018 is estimated to be 176113 tones. Camel produces considerable amount of milk and for long period of time than other milk producing animals under harsh conditions. Meat quality is largely age dependent and as other animals, good meat is from young slaughtered camel. Camel herd sizes per household vary from few heads to several hundreds. The proportion of male to female animals in breeding herd is not equal because of higher number of female camels in the herd as compared to male camels. During the dry season all female camels are shifted to areas with better feed accessibility but male camels are usually reared around settlement area. Mostly male camels are used for transportation of commodities. Breeding management comprises of selection of best animals for next generation and/or culling of the worst animals from the herd, and controlled breeding. Camels start breeding at the end of the dry season and the beginning of rainy season and continue thought the season. Camel can be categorized under seasonal breeding animal species. Prevalence of disease in pastoral areas of Ethiopia is principal constraint of the pastoralist in their camel production. There are inadequate researches and reports have been conducted on potential of camel production and management system in the country. Therefore, this review paper is going to present potential of camel production and management practices in Ethiopia. Key words: Camel, management practices, production potential.

The pandemic resulting from COVID-19 infections had short-term positive impacts on the environment such as improvement in air and water quality. However, long term changes still have disastrous effects in terms of loosening of conservation policies and an increase in ‘post-COVID-19’ development subsidies to boost the economy at the expense of the environment. The prevention of habitat loss and zoonoses will avert future pandemics and measures to protect the local environment should be taken. The Republic of Korea follows the global trend in the weakness of long-term environmental answer to the pandemic and other on-going zoonoses, such as the avian influenza and African swine fever. Some of the current activities may even increase the risks of pandemic as mass culling of animals is widespread despite known risks. Instead, environmental protection and decreased encroachment may be the only safe way to proactively prevent the emergence of further pandemics.

Context Cow survival measures the ability of cows to survive from the current to subsequent lactation. In addition to economic gain, genetic selection for survival could improve animal welfare by increasing the adaptability and resilience of the cows to both environmental and health challenges. However, survival is a complex trait because it results from a diverse range of reasons for culling of cows from the herd. Consequently, the accuracy of genetic predictions of direct survival are often low. Aims Our aim was to increase the accuracy of predictions of survival in Holstein and Jersey sires by including important predictor traits in multi-trait evaluation models. Methods Phenotypic and genetic correlations between survival trait deviations (TDs) and 35 routinely measured traits (including milk yield, fertility and type traits) were estimated using bivariate sire models. Survival TDs for 538 394 Holstein and 63 839 Jersey cows were used in our study; these cows or their close relatives also had milk, fertility and type traits records between 2002 and 2019. These genetic parameters were required to assess the potential usefulness of predictor traits for the prediction of survival. Key results Survival was genetically correlated with milk, fat and protein yields, overall type, composite mammary system and fertility TDs in both Holstein and Jersey. Further, most of the type traits related to feet and legs, and rump, were also correlated with survival TDs in Jersey. For sires, the accuracy of predictions for survival increased by 0.05 for Holsteins (from 0.54 to 0.59) and for Jerseys (from 0.48 to 0.53) through the use of multivariate models compared with univariate models. Conclusions Survival was genetically associated with traits affecting voluntary and involuntary culling and when included in multi-trait genetic evaluation models, they moderately improved the accuracy of genetic prediction of survival. Implications Predictor traits can be used to increase the accuracy of predictions of survival through the use of multi-trait models. The inclusion of breed-specific predictor traits should be considered, especially for Jerseys in genetic evaluations of survival.

Alien mammal species have a very long history of anthropogenic introductions and have been extensively studied due to their severe impact on native biodiversity and ecosystems. One of the most detrimental species is the sika deer (Cervus nippon), a native Asian deer that has settled in several districts of the Czech Republic and has widely colonized the area with increasing population numbers. In this study we aimed to investigate the trend of the number of sika deer culled in the country between 1994 and 2018, and to highlight limits and flaws in the current management and hunting strategy. We identified three hunting subpopulations based on both the official hunting records and the environmental and urban features of the Czech Republic. The analyses of the subpopulations over the period between 1994 and 2018 revealed very different trends, some of which are alarming. The average annual increase in culling was 7.4% for Subpopulation 1 (SP1), 1% for Subpopulation 2 (SP2), and 3.7% for Subpopulation 3 (SP3). Considered the increased percentages of culled deer, and the environmental features of the country, we evaluated the central subpopulation (SP2) as the easiest to manage due to its small area of extension, the scarcity of forested regions and, as possible contribution, the natural occurrence of predators. Being the other two subpopulations spread across larger forested areas and hosting a higher number of deer, we concluded that an increasing effort, and a more well-designed strategy to ensure accuracy of hunting plans seems to be impelling for the country.

Problems with claw disorders in dairy cattle herds are very typical and are among the most significant endemic cattle diseases. It is good to realize in what condition we keep the cattle - unsuitable floors and bed boxes, greasy microclimate, frequent movement between groups of animals, significant changes in the feed ration - all this affects the health of claws. Lameness and claw lesions have a great impact on production, welfare and health of dairy cows. They decreases milk yield, have a negative impact on reproductive traits and fertility performance and therefore lameness increases costs including medicine, detection of lameness, control and prevention, and creates losses including reduced milk production, non-sellable milk, reduced reproductive performance, increased culling and animal death. To reduce the effect of claw disorders on dairy cattle, it is necessary to apply the most efficient combination of available methods of prevention. The most effective is the combination of early detection and diagnosis, regular claw trimming, proper diet and clean and suitable enviroment. The aim of this review was to cumulate the existing informations of aspects of claw lessions and known preventive methods for breeders of dairy herds.

Since 2003, highly pathogenic avian influenza (HPAI) viruses of the H5 subtype have been maintained in poultry, periodically spilling back into wild migratory birds and spread to other geographic regions, with re-introduction to domestic birds causing severe impacts for poultry health, production and food sustainability. Successive waves of infection have also resulted in substantial genetic evolution and reassortment, enabling the emergence of multiple clades and subtypes within the H5 2.3.4.4 HPAI viruses. Control of AI is principally through either culling or through vaccination using conventional vaccines. Here, we antigenically and genetically characterise the emerging 2020/21 H5NX clade 2.3.4.4 strains and assess cross-reactivity to putative vaccine strains using chicken antisera. We demonstrate significant antigenic differences between commercially available poultry vaccines and currently circulating viruses suggesting that vaccination options might be suboptimal in the current outbreaks.

Bovine leukemia virus (BLV) infection, endemic in North American dairy herds, has production-limiting effects. A literature review of available papers published since 1995 concerning BLV transmission and its control was conducted. Although confirmed transmission routes were reviewed (blood, natural breeding, in utero, colostrum, and milk), there is still a lack of detailed information on other specific risks for transmission (e.g., contact transmission and hoof-trimming knives). Eradication of BLV has been achieved by combined management, segregation, and culling approaches. In contrast, although sole implementation of best management practices aimed at prevention of BLV transmission has decreased within-herd BLV prevalence, it has not eradicated BLV from a herd. Therefore, control and eradication of BLV by best management practices only should be further investigated. Additionally, the role of proviral load in infected cattle was investigated. Cattle with a high proviral load seem to be more likely to infect others, whereas those with a very low proviral load seem to have low risks of transmitting BLV. Information on proviral load could be taken into account when controlling BLV in high-prevalence herds. In conclusion, there is a need for detailed, large-scale studies investigating roles of specific transmission routes, knowing proviral load of infected individuals.

Physiological udder edema is a noninfectious metabolic disorder in dairy cattle, which may be present in a high percentage of dairy cows. This review summarizes the factors associated with udder edema. They include genetics, nutrition, oxidative stress, and physiological changes in freshening heifers. Udder edema negatively affects the productive life of a dairy cow. Udder support structures may be broken down due to tissue damage. Swollen teats may become sensitive, which makes attaching the milking unit more difficult. The amount of milk produced is decreased due to fluid buildup in the tissue spaces. Risk of secondary diseases, such as mastitis or udder cleft dermatitis, is also increased. All of these elements have an economic impact on the dairy farmer, in both the short term and the long term. If severe, damage could lead to early culling. Some possible methods for managing udder edema include (1) providing a separate diet for late-gestation heifers to monitor anionic salt intake, (2) selecting for either genetic lines with lower milk production or a phenotypic reduction of udder edema, and (3) ensuring that adequate exogenous antioxidants, such as vitamin E, vitamin C, carotenoids, and flavonoids, are provided in the diet to mitigate oxidative stress. In conclusion, udder edema may be an emerging issue that has the potential to seriously affect dairy cow welfare. Many of the research studies are outdated, and research with modern dairy cows is needed. The development of a scientifically validated udder edema scoring system is also needed to assess the severity of udder edema.

Purpose The purpose of this invited review was to identify and discuss (a) the welfare issues that can occur when cull dairy cows are sent for slaughter, (b) factors that can affect the occurrence and severity of these issues and how they relate to on-farm management decisions, (c) measures that can mitigate these issues, and (d) proposals to improve the welfare of cull dairy cows. Sources Peer-reviewed literature, book chapters, reports, and guidance documents were sources of information. Synthesis Severe welfare issues occur when some cull dairy cows that are not fit for the intended journey are transported to slaughter. These issues are even greater if compromised cows are sent to slaughter via an auction market. The decision to send a cull cow to slaughter needs to be made before the cow becomes unfit for the likely journey. If a cow becomes unfit for transport, it should be euthanized on the farm. Some cull cows arrive at slaughter plants and are observed at markets with distended udders, severe lameness, and disease. The numbers of cows with these severe welfare issues could be reduced by improved on-farm decision making about when and how to manage cows with health issues, when to euthanize sick or injured cows, and when a cow is fit for transport and for sale at a market. Dairy producers need to understand the welfare implications of how they manage their cull dairy cows, and that some changes in their management practices are required. Conclusions and Applications This review identified that research on the assessment of the fitness of cull dairy cows for transport and on the factors that influence their welfare during transport and marketing are required. Research about how to motivate dairy producers to incorporate the welfare implications of their management of cull cows into their culling decisions and how to increase their use of industry recommendations about the care of cull cows are priorities.

Our objective was to assess the effects of feeding negative dietary cation-anion difference (DCAD) prepartum diets on milk production, reproductive performance, and culling. Cows from 4 commercial farms in Ontario, Canada were enrolled in a pen-level controlled trial from November 2017 to April 2019. Close-up pens (1 per farm) with cows 3 wk before calving were randomly assigned to a negative DCAD (TRT; -108 mEq/kg of dry matter; target urine pH 6.0-6.5) or a control diet (CON; +105 mEq/kg of dry matter with a placebo supplement). Each pen was fed TRT or CON for 3 mo (1 period), and then switched to the other treatment for the next period (4 periods per farm). Data from 15 experimental units (8 pen treatments in TRT and 7 in CON), with a total of 1,086 observational units (cows), were included. The effect of treatment on milk yield at the first 3 milk recording tests of lactation was assessed with linear regression models accounting for repeated measures. The risk of pregnancy at first artificial insemination and culling by 30, 60, and 305 d in milk (DIM) were analyzed with logistic regression models, and effects on time to first AI, pregnancy, and culling were assessed with Cox proportional hazards models. All models included treatment, parity, and their interactions, accounting for pen-level randomization and clustering of animals within farm with random effects, giving 10 degrees of freedom for treatment effects. Multiparous cows fed TRT produced more milk at the first (42.0 vs. 38.8 ± 1.2 kg/d) and second (44.2 vs. 41.7 ± 1.3 kg/d) milk tests. However, multiparous cows fed TRT tended to have 0.2 percentage units less milk fat content at these tests. Although multiparous cows fed TRT tended to have greater energy-corrected milk at the first test (least squares means ± standard error: TRT = 46.1 ± 0.9 vs. CON = 43.8 ± 1 kg/d), there were no differences observed in energy-corrected milk at the second or third tests. In primiparous cows, there was no effect of treatment on milk production. Multiparous cows fed TRT had greater pregnancy to first insemination (TRT = 42 ± 3 vs. CON = 32 ± 4%) and tended to have shorter time to pregnancy [hazard ratio (HR) = 1.20; 95% CI: 0.96-1.49]. In primiparous cows fed TRT, time to pregnancy was increased (HR = 0.76; 95% CI: 0.59-0.99). Culling by 30 DIM tended to be less in TRT (3.3 ± 1.1%) than CON (5.5 ± 1.8%). No effect of treatment on culling by 305 DIM was detected in primiparous cows, but in multiparous cows, the TRT diets decreased the odds of culling (21.3 ± 1.9 vs. 31.7 ± 2.8%) and daily risk of culling to 305 DIM (HR = 0.64; 95% CI: 0.46 to 0.89). Under commercial herd conditions, prepartum negative DCAD diets improved milk production and reproductive performance, and reduced culling risk in multiparous cows. In primiparous cows, TRT diets had no effect on milk yield or culling, but increased the time to pregnancy. Our results suggest that negative DCAD diets should be targeted to multiparous cows.

The use of modern chicken genotypes with high egg or meat performance results in the ethically unacceptable practice of culling day-old male layer chicks because of their inefficient fattening performance. Dual-purpose genotypes with a balanced performance profile for both eggs and meat are one option to avoid this practice. In this study, four chicken crosses of a layer breed (White Rock or New Hampshire) and the meat breed Bresse Gauloise were compared under the conditions of organic agriculture. Purebred Bresse Gauloise and the layer hybrid Lohmann Sandy served as controls. Part 1 of this study focused on the fattening performance of the cockerels, which were reared together with the pullets. The birds were housed in a floor system (9.9–20.7 kg live weight per m2 at the end of week 15) with access to a green outdoor run. Live weight of the crosses before slaughter at the age of 15 weeks ranged between 2355 and 2447 g and did not differ significantly between the genotypes. With average daily gains of 22.1–22.8 g, the crosses grew slower than Bresse Gauloise males (26.1 g) but faster than Lohmann Sandy males (15.9 g). Welfare assessment indicated a generally high level of welfare with no foot pad lesions or hock burns on any of the cockerels. Fattening dual-purpose cockerels can therefore be an ethically desirable option with a high level of animal welfare and a better fattening performance than in male layer chicks, but still requires more resources than the fattening of specialised broilers.

The complex application of Ketoquin 10% and Paracetam AVZ preparations, watersoluble vitamin D 3 , as well as vitamin complexes Volstar or VitOkey in the system of veterinary preventive measures for replacement young stock of breeding poultry, increased the efficiency of hatching eggs production by reducing purchased at the dayold age of males by 30%, minimized their subsequent culling by 2.2 times, increased safety during rearing by 6.3% and their uniformity in live weight by 15.6%. At the same time, the uniformity of females in live weight increased by 2.3% compared to the control. Due to the synchronization of puberty, the productivity of chickens and the quality of hatching eggs increased.

Purpose: study the phenotypic parameters of fertility, milk production and survival of the same cows during their lifetime in the herd (from birth to the fourth calving) depending on the age of the first calving to better understand the factors that will improve the productive longevity of cows.Material and methods. Studies were conducted in a commercial herd with Holstein cattle located in the Central region of the Russian Federation. For the analysis, we used data from cows (culling and alive) of the same year of birth (2014), which were born, grew, calved and reached 4 calving (2019). The generated database contained complete information about the animal throughout its life in the herd (n=842). Depending on the actual age of the first calving (26 months), which reflects the growth rate, cows were grouped into groups: less than 23 months, 23–25 months, 26–29 months and over 30 months. In each group, we studied (1) reproductive parameters of heifers (number of inseminations per conception, age of the first insemination); (2) reproductive parameters of cows (number of inseminations per conception,, number of days from calving to first insemination, number of days from first to last insemination, conception rate at first insemination, number of days from calving to conception); (3) 305-day milk yield of first, second and third lactations; (4) lifetime milk production; (5) longevity index (proportion of days spent on milk production); (6) survival rate (proportion of cows surviving from first calving to the second, third and fourth calving, respectively). Results. Cows with an average age of the first calving of 22.1 (Conclusion. Cows with the age of the first calving ≤25 months without serious problems during the rearing period were distinguished by the best indicators of reproduction and productivity. They reached the third lactation faster and ended it by producing the largest amount of milk, which spent 45 to 48% of their productive life.

The effect of acoustic pulse technology (APT) on recovery, culling, milk yield, and economic benefits for 118 cows with subclinical mastitis was compared with a no-treatment control (59 vs. 59), and another 118 APT-treated cows with clinical mastitis were compared with antibiotic-treated controls (59 vs. 59). Recovery was defined as a decrease in somatic cell count (SCC) to &lt, 250 × 103 cells/mL in at least two out of three monthly milk recordings after treatments. For the subclinically infected cows, APT treatment resulted in 65.5% recovery, 0% culling, and additional milk yield of 2.74 L/cow per day compared to 35.6% recovery and 5.1% culling in the no-treatment controls. For the clinically infected cows, APT treatment resulted in 67.8% recovery, 6.8% culling, and additional milk yield of 3.9 L/cow per day compared to 35.6% recovery and 32.2% culling in the antibiotic-treated group. Bacteriological analysis was run for 95 (80%) cows with clinical mastitis (APT-46, AB-49). For cows with Escherichia coli infection, 85.7% (18/21) treated with APT recovered vs. 17.6% (3/17) in the antibiotic-treated group, for cows with streptococcal infection, 66.0% (12/18) in the APT-treated group recovered vs. 44.4% (8/18) in the antibiotic-treated group.

Context Smallholder beef farming in Lao People’s Democratic Republic (Laos) is constrained by poor reproductive performance, contributing to regional food insecurity. To address this, interventions were promoted in some rural communities by extension services to enhance cattle and buffalo nutrition, health, and reproductive husbandry. Aims This study assessed the impacts of these interventions on cattle and buffalo reproductive performance. Methods Knowledge, attitude and practice surveys were conducted on smallholder beef farmers in 2015 (n = 637) and 2018 (n = 226). With written records lacking on these farms, the survey relied on farmer recall of the number of calves born in the previous 12 months and the number of female cows (>12 months of age) present. The rate of these, calves/female cows, was used as an indicator of reproductive performance. Key results This proportion was higher in 2018 at a predicted mean 0.27 calves/cow compared with 2015 at 0.21 calves/cow (P < 0.001), suggesting a beneficial effect of interventions over time. Specifically, reproductive performance improved with increasing land dedicated to forages (P < 0.001) and increased farmer market-orientation (P = 0.006). Farmers who believed that selling sick large ruminants stopped the spread of disease had enhanced reproductive performance (P = 0.008). Although the practice of culling animals of low reproductive performance is desirable, in countries where foot-and-mouth disease is endemic, it is important to discourage practices that increase infectious disease transmission risk. Conclusion As reproductive knowledge interventions were not associated with reproductive performance, introducing interventions such as castration and weaning to consolidate reproductive knowledge is recommended as well as addressing challenges that limit forage adoption; and debunking misconceptions on effectiveness of biosecurity. Implications The use of evidence-based research helps inform the selection of interventions required to best enhance reproductive efficiency, potentially leading to improved reproductive performance of smallholder large ruminant systems in Laos and beyond.

Neospora caninum (N. caninum) infection, one of the major causes of abortions in dairy cattle, has brought a huge loss to farmers worldwide. In this study, we develop a six-compartment susceptible-infected model of N. caninum transmission which is later reduced to a two-equation system. Potential controls including medication, test-and-cull, and vaccination are proposed and analyzed, and the corresponding reproduction numbers are derived. The conditions for the global stabilities of disease-free and endemic equilibria are investigated with analytical solutions and geometric approach. Furthermore, uncertainty and sensitivity analysis shows that three control strategies are effective towards the varied environment, whereas the effectiveness of each measure highly depends on parameters related to control actions. Dynamics of reproduction numbers illustrate that disease elimination can be achieved by three types of controls: (1) adopting medication with medicine efficacy higher than 0.4 to prevent vertical transmission, (2) implementing test-and-cull with culling coverage larger than 0.3, and (3) taking vaccine with coverage larger than 0.1. Numerical results suggest that preventive measures should at least include the prevention of access of other hosts, such as dogs, to cattle; otherwise, these control measures will lose effectiveness. Our presented study provides guidance for decision-making on N. caninum infected farm management.

Calving difficulty is associated with cow mortality, calf loss, veterinary and labor costs, and causes delayed return to estrus and lower conception rate. Dystocia is common in heifers, and the frequency decreases with the increasing age of the cow. Farmers need to consider both genetics and management factors in an attempt to reduce dystocia. Calf birth weight, the pelvic area of the dam and their interrelationships are major determinants of dystocia. The weight of the calf is governed by genetic, environmental and management factors. Genetic factors include sex, length of gestation, breed, inbreeding, and genotype. Non-genetic factors include age and parity of the dam, nutrition of the dam during various phases of gestation, and environmental temperature and humidity. Dystocia management must begin with a selection of heifer for mating with respect to the body weight of heifers, body condition score (BCS) and dam’s pelvic area should be considered before heifer selection. Not only heifer selection but sire selection using an estimated breeding value for calving ease should also be considered to lessen the dystocia rate in a herd. A combination of culling heifers with small pelvic areas and using bulls reported to be calving ease sire may reduce dystocia significantly. Apart from all these factors, genomic selection for reducing calving difficulties is a new tool for dystocia management.

Livestock production is a key element for poverty alleviation, food security, and economic growth in Rwanda. In 2017, the national average milk production per cow was about 2.5 L per day; in 2020–2021, it is projected to increase to 3.5 L per day if improvement interventions including those designed to reduce the burden of brucellosis in cattle are implemented. The objective of the study reported here was to estimate the seroprevalence of and identify risk factors associated with dairy farms and cattle classified as seropositive to Brucella spp. in three different agroecological zones in Rwanda. Most study farms (40/85 or 47%) had one head of cattle only. Using the Rose Bengal test, the seroprevalence of brucellosis was 28/85 or 33% (95% CI = 24%, 43%) at the farm level and 63/465 or 14% (95% CI = 11%, 17%) at the animal level. Using logistic regression, at the farm level, the presence of seropositive cattle was associated with herd size (2–45 cattle, odds ratio = 21.2; 95% CI = 2.4, 184.5) (46–220 cattle, OR = 288.5; 95% CI = 24.3, 3,423.1) compared to farms with one animal, after controlling for main breed (local breeds, crossbreeds) on the farm. In addition, the odds of testing seropositive were 10.7 (95% CI = 2.3, 49.1) and 149.5 (95% CI = 19.3, 1,158.7) times higher in farms in Nyabihu district and Nyagatare district, respectively, than in farms in Muhanga district, after controlling for main breed on the farm. The odds of seropositivity to Brucella spp. were 2.8 times higher in farms with mostly local breeds, than in those with mostly crossbreeds; but the association was confounded by herd size and geographic location. At the animal level, the odds of seropositivity to Brucella spp. were 2.6 times higher in adult cattle than in young cattle (95% CI = 1.1, 6.3). Finally, we observed a high frequency of adult cattle (86%) and a high seroprevalence of brucellosis in adult cattle (25%) in Nyagatare; an indication that, in the absence of culling and other control measures, Brucella spp. infection pressure can be relatively constant and a steady source of disease transmission in pastoral systems in that district.

The article analyzes the influence of the season of the year on qualitative and quantitative sperm parametres of servicing bulls, it also considers the structure of reasons for culling of ejaculates. The research was carried out on bulls of Holstein, Black Spotted, brown Swiss and Hereford breeds at the age of 2-6 years in OOO “Nizhegorodskoe” for breeding work”. Statistically significant influence of the month of the year on concentration and activity of spermium, the ejaculate volume, the proportion of rejection of ejaculates that do not meet the requirements was established. The sperm concentration was 0.592 billion / ml at the beginning of the year. In spring, there was an increase in concentration and a sharp jump in June to 0.886 billion / ml, then it gradually decreased. The average volume of ejaculate at the beginning of the year was quite high 7.69-8.45 ml, in June there is a sharp decrease in volume, in subsequent months there was a gradual increase in volume to 7.68 ml. The sperm activity parametres change slightly during the year. At the beginning of the year, sperm activity was 5.98 points, in March there is an increase to 6.33 points, and in July to 6.47 points. After July, there is a decrease in activity to 5.95 points. The month influence on sperm concentration was 28.18 %, on the average volume of ejaculate - 19.73 %, on sperm activity - 6.36 %, on the number of frozen semen doses - 10.49 %. The most intensive culling of native sperm occurs in winter-spring months - 16-18 %, in summer culling is reduced to 3-8 %. Thus, in June, the maximum number of semen doses per a servicing bull was received - 887.4. The main reason for culling throughout the year was a low concentration of sperm in the ejaculate, more than half of the culled ejaculates (54.3 %) had this cause, 26.6 % - low activity, 13.6 % - both defects at the same time, 5.5 % were culled for other reasons

Records of cattle vaccination against paratuberculosis (PTB) have been analyzed to determine whether or not non-specific effect (NSE) on overall mortality similar to that observed in BCG vaccinated humans occurs in animals. The results of a previously reported slaughterhouse study on PTB prevalence were used as a reference on the age incidence of advanced patent (clinical) epidemio-pathogenic forms. In the proper vaccine study, cows in 30 cattle farms in the Basque Country, Spain were followed-up for between 1 and 13 years. Vaccinated groups were composed by 1008 (592 right-censored) animals younger than 3 months treated as calves and by 3761 (3160 right-censored) vaccinated at any older age. Controls were 339 (157 right-censored) and 4592 (2213 right-censored) age matched animals, respectively. Individual last year presence in the annual testing was considered age at culling or death. A survival analysis was carried out according age at vaccination of vaccinated versus non-vaccinated animals. PTB age incidence in the slaughterhouse study was subtracted from the difference between vaccinated and non-vaccinated animals at the same age in order to estimate PTB-specific and non-specific effects. The maximum difference was observed at the 2–3 years interval with a 33.9% mortality reduction in the calf vaccinated group. This corresponded also with the maximum NSE that was 24.5% for a PTB incidence of 9.5%. Overall, vaccination afforded to calves a 26.5% yearly mortality protection, split between 11.1% PTB-specific and 15.4% NSE. These results support a NSE on total mortality associated with PTB vaccination that appeared to persist for up to 6–7 years. This confirms for the first time in an animal field study the innate immune system memory predicted by the recently proposed trained immunity theory. Contrasting the literature, no deleterious effects of killed vaccines on females were observed. Mortality reduction would offset vaccination costs and could improve livestock systems efficiency and potentially reduce antibiotic use. Clinical trial registered with Spanish Agency for Drugs and Sanitary products (AEMPS) as 11/012/ECV.

Reproductive tract diseases can be a significant cause of subfertility or infertility in male small ruminants. Affected males can usually be identified early by performing routine serologic screening and yearly breeding soundness examinations. Early diagnosis will either maximize treatment success or expedite culling decisions. Once clinical signs of diseases develop, prognosis for breeding ability declines. Veterinary practitioners should used ancillary diagnostic techniques, such as ultrasound examination and laboratory testing to acquire a diagnosis and determine prognosis of reproductive lesions. Some diseases (eg, brucellosis) should be ruled out immediately to cull affected animals and minimize transmission within the herd.

The objective of this study was to quantify the efficacy of a second intrauterine cephapirin treatment administered 14 d after the initial one on subsequent reproductive performance of postpartum dairy cows affected by purulent vaginal discharge (PVD) or endometritis (ENDO). In total, 4,140 Holstein cows from 30 commercial herds were enrolled in a randomized clinical trial. At 36 (±7) d in milk, cows were examined using the Metricheck device to diagnose PVD. An endometrial cytology sample was also collected from each cow to perform a leukocyte esterase test for diagnosing ENDO. Diagnosis of PVD and ENDO was done cow-side. Cows diagnosed with PVD or ENDO were assigned to receive 1 of 2 treatments: (1) a single intrauterine cephapirin infusion (500 mg of cephapirin benzathin; Metricure, Merck Animal Health, Kirkland, QC, Canada) at the time of initial examination or (2) a single intrauterine infusion at the time of initial examination and a second one 14 d later. Subsequent reproductive and culling events were collected until 200 d in milk. Statistical analyses were performed using univariable and multivariable mixed logistic regression models. In cows affected by PVD, a second intrauterine cephapirin infusion increased the pregnancy risk at first insemination in comparison with cows that only received one treatment (28.0 vs. 38.8%). In cows affected by ENDO, a second treatment also increased the pregnancy risk at first insemination compared with cows that only received one treatment (30.3 vs. 39.2%). Overall, these results demonstrate that administering a second intrauterine cephapirin infusion 14 d after the initial treatment in postpartum cows affected by PVD or ENDO did improve their subsequent reproductive performance.

A total of 792 litters from 210 does of Baladi Black (BB) rabbits were considered to make characterization, and genetic evaluation for some doe traits, including litter traits (litter size at birth; LSB, litter size born alive; LSA, litter size at 21 days; LS21, litter size at weaning; LSW, Litter weight at birth; LWB, Litter weight at weaning; LWW, and the pre-weaning mortality rate; PWM), conception traits (number of services per conception; NSC, and gestation length; GL), and interval traits (kindling interval; KI and days open; DO). Heritability for litter traits was relatively low ranged from 0.08 to 0.14 except this for PWM it was moderate (0.15); h2 for conception and interval traits were low ranged from 0.00 to 0.06. Repeatability (R) for litter traits were low to moderate ranged from 0.11 to 0.34; R estimates for PWM and LWB were higher than other traits (0.16 and 0.34) respectively; and R for conception and interval traits was low ranged from 0.01 to 0.11. Genetic correlations among litter traits were positive, and moderate to strong ranged from 0.23 to 0.92 except for this between LSB and LWW and this between LWB and PWM, it was negative -0.16 and -0.08 respectively. Genetic correlations among conception and interval traits were positive and very strong ranged from 0.68 to 1.00 except this between GL and NSC it was negative and strong (-0.77). The ranges of transmitting abilities (TA) for litter size traits ranged from 1.92 to 2.10 kits. Ranges of TA for litter weight traits ranged from 0.01 to 0.70 grams. The range of TA for PWM was 33.90%. Ranges of TA for conception traits were low, 0.08 days for GL and 0.11 (numbers) for NSC. The ranges of TA were 4.93 and 10.72 days for KI and DO respectively. The percentage of positive transmitting ability ranged from 30.95 to 53.81 for litter traits and from 50.00 to 51.90 for conception, and interval traits. The genetic trends for litter traits at different ages, conception and interval traits were low and had fluctuations by year-season which could be due to variation in climatic conditions. Conclusively, Baladi Black rabbits' does have good potential reproduction. Most of doe reproductive traits of these rabbits were largely affected by environmental conditions which would be improved by improving environmental and management conditions. The moderate estimate of h2 for PWM (0.15) and its range of transmitting ability (33.90%) suggest decreasing pre-weaning mortality by selection. Moderate estimates for PWM and LWB comparing with other traits (0.16 and 0.34) respectively suggests using culling and selection strategies. The negative genetic correlation between LSB and LWW (-0.16) should be taken into consideration when designing a breeding program for these rabbits. The perfect genetic correlation between KI and DO (1.00) reflected that these two traits could be indicators of each other when designing breeding programs.

Mycoplasma bovis, a disease affecting cattle worldwide, was first reported in New Zealand in 2017. Classed as an unwanted organism, the Government attempted eradicating it via culling of infected h...