78 results on '"Huizhen Sun"'
Search Results
2. Thoracic interstitial injection of drug-liposomes in mice for treating atherosclerosis
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Tun Yan, Huizhen Sun, Yahong Shi, Ya Gao, Xi Lu, Kai Li, Yuting Zhu, Qiang Zhang, Tingting Li, Zhongxian Li, Xiaohan Zhou, Yuting Guo, Yinglu Ji, Xiaoli Shi, and Dong Han
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General Materials Science ,Electrical and Electronic Engineering ,Condensed Matter Physics ,Atomic and Molecular Physics, and Optics - Published
- 2022
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3. Role of S protein transmembrane domain mutations in the development of occult hepatitis B virus infection
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Xinyi Jiang, Le Chang, Ying Yan, Huimin Ji, Huizhen Sun, Yingzi Xiao, Shi Song, Kaihao Feng, Abudulimutailipu Nuermaimaiti, and Lunan Wang
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Hepatitis B virus ,Hepatitis B Surface Antigens ,Epidemiology ,Immunology ,General Medicine ,Hepatitis B ,Microbiology ,Hepatitis B, Chronic ,Infectious Diseases ,Virology ,DNA, Viral ,Mutation ,Drug Discovery ,Humans ,Parasitology ,Phospholipids - Abstract
Occult HBV infection (OBI) is a special infection status during Hepatitis B virus (HBV) infection. The underlying mechanism of its occurrence remains unclear. This study conducted sequencing analysis on 104 OBI plasma samples and 524 HBsAg positive samples from 29 blood centres, and searched for high-frequency mutations in transmembrane domain (TMD) of S protein in the OBI population. Plasmids with TMD high-frequency mutations were constructed, in vivo and in vitro functional experiments were performed to investigate possible molecular mechanisms of OBI occurrence. We found 22 high-frequency TMD mutations in genotype B OBI strains. Among them, five mutations can lead to impairment of HBsAg secretion; seven mutations had accumulated intracellular HBsAg while extracellular HBsAg didn’t decrease compared to wildtype. This study chose C85R from TMD2, F220C, and F220Y from TMD4 for further exploration. Protein structure predication showed these three mutant HBsAg displayed changed hydrophilic properties and tended to accumulate in the phospholipid bilayer of cell membrane. Mutant HBsAg’s secretion disorder may induce OBI. On the other hand, V168A + V177A from TMD3 expressed increased HBsAg both in intracellular and extracellular levels. This mutation had most unstable natural conformation and may be inclined to transition into V177A or V168A + S174N + V177A. These three mutations were more prone to mixed infection, presenting a state of coexistence, thus approaching the impaired secretion pattern of OBI. This study demonstrated TMD mutations could contribute to the occurrence of OBI and provided a theoretical basis for OBI study and the functional cure of chronic hepatitis B virus infection.
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- 2022
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4. Performance of ferroelectric visible light type II Ag10Si4O13/TiO2 heterojunction photocatalyst
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Cuixia Li, Huizhen Sun, Haize Jin, Wenshen Li, Jingbo Louise Liu, and Sajid Bashir
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General Chemistry ,Catalysis - Published
- 2022
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5. Seasonal non-structural carbohydrate dynamics differ between twig bark and xylem tissues
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Liyuan Gao, Doug P. Aubrey, Xingchang Wang, and Huizhen Sun
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Ecology ,Physiology ,Forestry ,Plant Science - Published
- 2022
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6. The Fibrillin‐1/VEGFR2/STAT2 signaling axis promotes chemoresistance via modulating glycolysis and angiogenesis in ovarian cancer organoids and cells
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Ziliang, Wang, Wei, Chen, Ling, Zuo, Midie, Xu, Yong, Wu, Jiami, Huang, Xu, Zhang, Yongheng, Li, Jing, Wang, Jing, Chen, Husheng, Wang, and Huizhen, Sun
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Mice, Knockout ,Ovarian Neoplasms ,Vascular Endothelial Growth Factor A ,Cancer Research ,Neovascularization, Pathologic ,Fibrillin-1 ,Mice, Nude ,STAT2 Transcription Factor ,Vascular Endothelial Growth Factor Receptor-2 ,Organoids ,Mice ,Oncology ,Drug Resistance, Neoplasm ,Cell Line, Tumor ,Animals ,Humans ,Female ,Cisplatin ,Glycolysis ,Zebrafish - Abstract
Chemotherapy resistance is a primary reason of ovarian cancer therapy failure; hence it is important to investigate the underlying mechanisms of chemotherapy resistance and develop novel potential therapeutic targets.RNA sequencing of cisplatin-resistant and -sensitive (chemoresistant and chemosensitive, respectively) ovarian cancer organoids was performed, followed by detection of the expression level of fibrillin-1 (FBN1) in organoids and clinical specimens of ovarian cancer. Subsequently, glucose metabolism, angiogenesis, and chemosensitivity were analyzed in structural glycoprotein FBN1-knockout cisplatin-resistant ovarian cancer organoids and cell lines. To gain insights into the specific functions and mechanisms of action of FBN1 in ovarian cancer, immunoprecipitation, silver nitrate staining, mass spectrometry, immunofluorescence, Western blotting, and Fӧrster resonance energy transfer-fluorescence lifetime imaging analyses were performed, followed by in vivo assays using vertebrate model systems of nude mice and zebrafish.FBN1 expression was significantly enhanced in cisplatin-resistant ovarian cancer organoids and tissues, indicating that FBN1 might be a key factor in chemoresistance of ovarian cancer. We also discovered that FBN1 sustained the energy stress and induced angiogenesis in vitro and in vivo, which promoted the cisplatin-resistance of ovarian cancer. Knockout of FBN1 combined with treatment of the antiangiogenic drug apatinib improved the cisplatin-sensitivity of ovarian cancer cells. Mechanistically, FBN1 mediated the phosphorylation of vascular endothelial growth factor receptor 2 (VEGFR2) at the Tyr1054 residue, which activated its downstream focal adhesion kinase (FAK)/protein kinase B (PKB or AKT) pathway, induced the phosphorylation of signal transducer and activator of transcription 2 (STAT2) at the tyrosine residue 690 (Tyr690), promoted the nuclear translocation of STAT2, and ultimately altered the expression of genes associated with STAT2-mediated angiogenesis and glycolysis.The FBN1/VEGFR2/STAT2 signaling axis may induce chemoresistance of ovarian cancer cells by participating in the process of glycolysis and angiogenesis. The present study suggested a novel FBN1-targeted therapy and/or combination of FBN1 inhibition and antiangiogenic drug for treating ovarian cancer.
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- 2022
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7. Particle-size-dependent biological distribution of gold nanoparticles after interstitial injection
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Huizhen Sun, Dong Han, Ya Gao, Tun Yan, Tingting Li, Yahong Shi, Songkun Gao, Zhongxian Li, Yuting Guo, and Xiaoli Shi
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Materials Chemistry ,General Materials Science - Abstract
There are significant differences in the biological distribution of AuNPs48 and AuNPs88 after interstitial injection, suggesting that we should consider the size effect of drugs when designing nanodrugs through interstitial injection.
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- 2022
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8. TRAF6 promotes chemoresistance to paclitaxel of triple negative breast cancer via regulating PKM2-mediated glycolysis
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Han Xu, Longzhi Li, Bing Dong, Ji Lu, Kun Zhou, Xiaoxin Yin, and Huizhen Sun
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Ample evidence reveals that glycolysis plays an important role in cancer progression; however, the underlying mechanism of its drug resistance is still worth being further explored. TRAF6, a E3 ubiquitin ligase, is well known to overexpress in various types of cancers, which predicts poor prognosis. In our study, we discovered that TRAF6 expressed more significantly in triple negative breast cancer (TNBC) than in other subtypes of breast cancers, promoting chemoresistance to paclitaxel; that the inhibited TRAF6 expression in the chemoresistant TNBC (TNBC-CR) cells enhanced the sensitivity by decreasing glucose uptake and lactate production; that TRAF6 regulated glycolysis and facilitated chemoresistance via binding directly to PKM2; and that overexpressing PKM2 in the TNBC-CR cells with TRAF6 knocked down regained significantly TRAF6-dependent drug resistance and glycolysis. Additionally, we verified that TRAF6 could facilitate PKM2-mediated glycolysis and chemoresistance in the animal models and clinical tumor tissues. Thus, we identified the novel function of TRAF6 to promote glycolysis and chemoresistance in TNBC by regulating PKM2, which could provide a potential therapeutic target for TNBC treatment.
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- 2023
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9. Low seroprevalence of hepatitis delta virus co-infection in hepatitis B virus-infected blood donors in China: A multicenter study
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Le Chang, Ying Yan, Huimin Ji, Huizhen Sun, Xinyi Jiang, Zhuoqun Lu, Lunan Wang, and HBV-Infected Blood Donors Study Group
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Microbiology (medical) ,Microbiology - Abstract
Hepatitis delta virus (HDV) coinfected with HBV causes severe viral hepatitis, however, the number of HDV infection may be underestimated. In the present study, we enrolled 1,141,331 blood donations, routinely tested for HBsAg and/or HBV DNA, from 21 blood establishments in China. 2,690 donors were HBsAg and/or HBV DNA positive after screening tests. After verification of HBsAg and HBV DNA, 1,490 samples were HBsAg confirmed-positive, including 1,459 HBV DNA-positive samples, and 825 samples were seronegative but HBV DNA positive. We first analyzed demographic characteristics of involved 2,690 donors with different HBV infection status and found the proportions of males, the older donors, workers and farmers were higher in HBsAg-/HBV DNA+ group. Then we evaluated specificity of HDV IgG and IgM antibody assays with 375 HBsAg and HBV DNA confirmed-negative samples, and 374 were tested negative using the two assays, respectively, suggesting a specificity of 99.73% for both assays (374/375, 95% Cl: 98.51–99.95%). Subsequently, we tested for HDV IgG and IgM of 2,315 HBsAg and/or HBV DNA confirmed-positive samples, and nine showed reactivity for IgG, while two were reactive for IgM. All these 11 reactive samples were tested again with another HDV pan-Ig and IgM testing assays and HDV RNA, and only one donor was identified as HDV IgG positive and HDV RNA negative, showing an HDV seroprevalence of 0.067% (95%CI: 0.012–0.38%) among HBsAg-positive blood donors in China. The positive donor was followed up for 2 years after the donation date, and decreased antibody titer of HDV IgG and HBsAg conversion were observed, and the infection status of the donor was HDV infection with recovery and occult hepatitis B virus infection with genotype C2. These results indicated a low seroprevalence of HDV infection among blood donors and a low risk of HDV transmission through blood transfusion in China.
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- 2022
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10. Circulating immune complexes and mutations of HBsAg are associated with the undetectable HBsAg in anti-HBs and HBeAg positive occult hepatitis B virus infection
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Ying Yan, Huizhen Sun, Le Chang, Huimin Ji, Xinyi Jiang, Shi Song, Yingzi Xiao, Kaihao Feng, Abudulimutailipu Nuermaimaiti, Zhuoqun Lu, and Lunan Wang
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Microbiology (medical) ,Microbiology - Abstract
IntroductionOccult hepatitis B virus infection (OBI) is an HBsAg negative state in HBV infection with usually inactive HBV replication. However, there were a minority of individuals with positive HBeAg and anti-HBs among OBI blood donors and few studies have focused on this unusual serological pattern.Methods2022 plasma of blood donors that preliminary screened reactive for HBV DNA and non-reactive for HBsAg were collected from 16 provinces in China from 2015 to 2018. HBV DNA and HBsAg in these samples were retested using the Cobas TaqScreen MPX test and ARCHITECT HBsAg Quantitative II assay. Lumipulse HBsAg-HQ assay and polyethylene glycol (PEG)-double precipitation following HCl and trypsin digestion were performed to detect HBsAg from HBsAg-anti-HBs circulating immune complexes (CICs).Results1487 of 2022 samples were positive for Cobas HBV DNA test and non-reactive for ARCHITECT HBsAg assay, while 404 of them were positive using Lumipulse HBsAg-HQ assay. 10 HBsAg-/anti-HBs+/HBeAg+ OBI blood donor samples were further dissociated and HBsAg-CICs were detected in 7 samples. Sequencing analysis showed that D44N, N98T, G73S, Del 56-116, and I161T occurred in the pre-S region, and immune escape mutations such as P127T, F134L, G145R, V168A, and I126T/S in the S region were found.DiscussionIn conclusion, there were a minority of HBsAg-/anti-HBs+/HBeAg+ individuals in OBI blood donors. The undetectable HBsAg in these individuals was mainly due to HBsAg-CICs. Immune escape-associated mutations also happened under the host’s selective pressure. HBsAg dissociation methods or Lumipulse HBsAg-HQ assay is recommended to distinguish these individuals.
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- 2022
11. Cordycepin protects islet β-cells against glucotoxicity and lipotoxicity via modulating related proteins of ROS/JNK signaling pathway
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Baiyi Yan, Yanchun Gong, Wei Meng, Huizhen Sun, Wenxi Li, Kaizhi Ding, Caixia Dang, Xiaofei Gao, Wei Sun, Chunhua Yuan, Songhua Wang, and Li-Hua Yao
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Pharmacology ,General Medicine - Published
- 2023
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12. Naturally occurring pre-S mutations promote occult HBV infection by affecting pre-S2/S promoter activity
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Huizhen Sun, Le Chang, Ying Yan, Huimin Ji, Xinyi Jiang, Shi Song, Yingzi Xiao, Zhuoqun Lu, and Lunan Wang
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Pharmacology ,Hepatitis B virus ,Hepatitis B Surface Antigens ,Hepatitis B, Chronic ,Genotype ,Virology ,Mutation ,DNA, Viral ,Humans ,Hepatitis B - Abstract
Occult hepatitis B virus (HBV) infection (OBI) has non-negligible clinical significance, but the mechanism of its occurrence remains unclear. Growing evidence suggests that mutations in the pre-S region of HBV genome may be associated with the occurrence of OBI. However, the role of pre-S mutations in OBI and its molecular mechanism was not fully understand. Here, the pre-S sequences from 307 OBI blood donors and 293 hepatitis B surface protein (HBsAg)-positive blood donors were obtained, and we observed a higher frequency of naturally occurring pre-S mutations in OBI donors infected with genotype B/C HBV than in HBsAg-positive donors, suggesting their potential positive role in OBI. In both genotype B and C, several pre-S mutants resulted in markedly reduced HBsAg production in vitro. In particular, the T68I, S78N and N98T mutants of genotype B were proven to significantly decrease the HBsAg synthesis by affecting the pre-S2/S promoter activity, and thereby promoting the occurrence of OBI.
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- 2022
13. Multiple modification on nanoporous silicon derived from photovoltaic silicon cutting waste for extraction of PbⅡ in industrial effluents
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Kaixin Fu, Ziheng Yang, HuiZhen Sun, Xiuhua Chen, Shaoyuan Li, Wenhui Ma, and Ran Chen
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Mechanics of Materials ,Materials Chemistry ,General Materials Science - Published
- 2023
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14. Novel strategy of combined interstitial macrophage depletion with intravenous targeted therapy to ameliorate pulmonary fibrosis
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Zhongxian Li, Qiang Zhang, Jiawei Xiang, Mingyuan Zhao, Yuan Meng, Xuhao Hu, Tingting Li, Yifeng Nie, Huizhen Sun, Tun Yan, Zhuo Ao, and Dong Han
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Biomaterials ,Biomedical Engineering ,Bioengineering ,Cell Biology ,Molecular Biology ,Biotechnology - Published
- 2023
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15. LRG1 Is Involved in the Progression of Ovarian Cancer via Modulating FAK/AKT Signaling Pathway
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Huizhen Sun, Xin Chen, Weiwei Xie, and Dongling Wu
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General Immunology and Microbiology ,General Medicine ,General Biochemistry, Genetics and Molecular Biology - Published
- 2023
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16. CDK4/6 inhibitor palbociclib promotes SARS-CoV-2 cell entry by down-regulating SKP2 dependent ACE2 degradation
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Yingzi Xiao, Ying Yan, Le Chang, Huimin Ji, Huizhen Sun, Shi Song, Kaihao Feng, Abudulimutailipu Nuermaimaiti, Zhuoqun Lu, and Lunan Wang
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Pharmacology ,Virology - Published
- 2023
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17. Stanniocalcin 1 promotes metastasis, lipid metabolism and cisplatin chemoresistance via the FOXC2/ITGB6 signaling axis in ovarian cancer
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Feikai, Lin, Xiaoduan, Li, Xinjing, Wang, Huizhen, Sun, Ziliang, Wang, and Xipeng, Wang
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Ovarian Neoplasms ,Cancer Research ,Integrin beta Chains ,Forkhead Transcription Factors ,Lipid Metabolism ,Gene Expression Regulation, Neoplastic ,Phosphatidylinositol 3-Kinases ,Oncology ,Drug Resistance, Neoplasm ,Cell Line, Tumor ,Humans ,Female ,Cisplatin ,Neoplasm Metastasis ,Glycoproteins ,Signal Transduction - Abstract
Background Stanniocalcin 1 (STC1) plays an integral role in ovarian cancer (OC). However, the functional role of STC1 in metastasis, lipid metabolism and cisplatin (DDP) chemoresistance in OC is not fully understood. Methods Single-cell sequencing and IHC analysis were performed to reveal STC1 expression profiles in patient tissues. Metastasis, lipid metabolism and DDP chemoresistance were subsequently assessed. Cell-based in vitro and in vivo assays were subsequently conducted to gain insight into the underlying mechanism of STC1 in OC. Results Single-cell sequencing assays and IHC analysis verified that STC1 expression was significantly enhanced in OC tissues compared with para-carcinoma tissues, and it was further up-regulated in peritoneal metastasis tissues compared with OC tissues. In vitro and in vivo experiments demonstrated that STC1 promoted metastasis, lipid metabolism and DDP chemoresistance in OC. Simultaneously, STC1 promoted lipid metabolism by up-regulating lipid-related genes such as UCP1, TOM20 and perilipin1. Mechanistically, STC1 directly bound to integrin β6 (ITGB6) to activate the PI3K signaling pathway. Moreover, STC1 was directly regulated by Forkhead box C2 (FOXC2) in OC. Notably, targeting STC1 and the FOXC2/ITGB6 signaling axis was related to DDP chemoresistance in vitro. Conclusions Overall, these findings revealed that STC1 promoted metastasis, lipid metabolism and DDP chemoresistance via the FOXC2/ITGB6 signaling axis in OC. Thus, STC1 may be used as a prognostic indicator in patients with metastatic OC. Meanwhile, STC1 could be a therapeutic target in OC patients, especially those who have developed chemoresistance to DDP.
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- 2022
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18. Aurora-A/SOX8/FOXK1 signaling axis promotes chemoresistance via suppression of cell senescence and induction of glucose metabolism in ovarian cancer organoids and cells
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H. W. Wang, Huizhen Sun, Xipeng Wang, Xinjing Wang, Xi Cheng, Yufei Yang, Xue Wang, Yoichi Aoki, and Ziliang Wang
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0301 basic medicine ,Senescence ,Cell ,Mice, Nude ,Medicine (miscellaneous) ,Antineoplastic Agents ,Aurora-A ,03 medical and health sciences ,0302 clinical medicine ,Ovarian cancer ,Cell Line, Tumor ,medicine ,Animals ,Humans ,Telomerase reverse transcriptase ,Pharmacology, Toxicology and Pharmaceutics (miscellaneous) ,Transcription factor ,Cellular Senescence ,Aurora Kinase A ,Cell Proliferation ,Ovarian Neoplasms ,Cisplatin ,Mice, Inbred BALB C ,SOXE Transcription Factors ,Chemistry ,Kinase ,Forkhead Transcription Factors ,medicine.disease ,Xenograft Model Antitumor Assays ,Gene Expression Regulation, Neoplastic ,Organoids ,Glucose ,030104 developmental biology ,medicine.anatomical_structure ,Drug Resistance, Neoplasm ,030220 oncology & carcinogenesis ,PDOs ,Cancer research ,Phosphorylation ,Female ,SOX8 ,Chemoresistance ,Signal Transduction ,Research Paper ,medicine.drug - Abstract
Rationale: Cisplatin derivatives are first-line chemotherapeutic agents for epithelial ovarian cancer. However, chemoresistance remains a major hurdle for successful therapy and the underlying molecular mechanisms are poorly understood at present. Methods: RNA sequencing of organoids (PDO) established from cisplatin-sensitive and -resistant ovarian cancer tissue samples was performed. Glucose metabolism, cell senescence, and chemosensitivity properties were subsequently examined. Immunoprecipitation, mass spectrometry, Fӧrster resonance energy transfer-fluorescence lifetime imaging (FRET-FLIM), luciferase reporter assay, ChIP and animal experiments were conducted to gain insights into the specific functions and mechanisms of action of the serine/threonine kinase, Aurora-A, in ovarian cancer. Results: Aurora-A levels were significantly enhanced in cisplatin-resistant PDO. Furthermore, Aurora-A promoted chemoresistance through suppression of cell senescence and induction of glucose metabolism in ovarian cancer organoids and cells. Mechanistically, Aurora-A bound directly to the transcription factor sex determining region Y-box 8 (SOX8) and phosphorylated the Ser327 site, in turn, regulating genes related to cell senescence and glycolysis, including hTERT, P16, LDHA and HK2, through enhancement of forkhead-box k1 (FOXK1) expression. Conclusions: Aurora-A regulates cell senescence and glucose metabolism to induce cisplatin resistance by participating in the SOX8/FOXK1 signaling axis in ovarian cancer. Our collective findings highlight a novel mechanism of cisplatin resistance and present potential therapeutic targets to overcome chemoresistance in ovarian cancer.
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- 2020
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19. Nitrogen doped porous carbon with high rate performance for lithium ion storage
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Huizhen Sun, Mingjun Xiao, and Fuliang Zhu
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General Chemical Engineering ,Electrochemistry ,Analytical Chemistry - Published
- 2023
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20. High-efficiency mechanically assisted alkaline extraction of nanoparticles from biological tissues for spICP-MS analysis
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Ya Gao, Ruiyi Zhang, Huizhen Sun, Yuting Guo, Lan Chen, Xiaoli Shi, and Guanglu Ge
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Mice ,Silver ,Animals ,Metal Nanoparticles ,Nanoparticles ,Tissue Distribution ,Gold ,Particle Size ,Biochemistry ,Analytical Chemistry - Abstract
The widespread use and increased exposure of nanoparticles call for technology to quantify their concentration and size distribution in biological matrices. As ex situ evaluation, facile extraction with high fidelity and efficiency is critical. In this work, single particle inductively coupled plasma mass spectrometry (spICP-MS) was used for nanoparticle number and distribution analysis, where a facile and highly efficient mechanically assisted alkaline digestion has been developed to extract nanoparticles at low alkali concentration. The optimization was performed using chicken tissues in vitro mixed with 30 nm gold nanoparticles, mixture of 30 nm and 60 nm gold nanoparticles, and 45 nm silver nanoparticles, respectively, which is, then, mechanically ground to form tissue homogenate and 2% TMAH is added. The nanoparticles are extracted with a recovery of more than 94% for all the spiked nanoparticle tissue samples. The extraction method has also been attempted to be applied to extract single-sized gold nanoparticles from various organs of mice mixed in vivo with the nanoparticles through intravenous injection, and led to consistent results with acid digestion. Mice injected intravenously with double-sized gold nanoparticle mixture were also studied, further showing that gold nanoparticles of 30 nm and 60 nm have no significant difference in their biodistribution in the same organ. To the best of our knowledge, this is the first attempt for multiple nanoparticles being extracted simultaneously and measured quantitatively from various organs, such as the heart, liver, spleen, lungs, and kidneys. We believe this method is beneficial to the safety assessment and toxicokinetics studies for nanoparticles in tissues.
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- 2021
21. Urodynamic Findings in Pediatric Spastic Cerebral Palsy: Retrospective Study From a Single Center
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Sen Li, Bo Xiao, Qijia Zhan, Min Wei, Huizhen Sun, Baojun Gu, Wenbin Jiang, and Fang Chen
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Pediatrics ,medicine.medical_specialty ,Spastic cerebral palsy ,business.industry ,medicine ,Retrospective cohort study ,medicine.disease ,Single Center ,business - Abstract
Objective To investigate the urodynamic study (UDS) result in pediatric patients with spastic cerebral palsy (CP). Material and methods Medical records of CP with pre-operative UDS results underwent selective dorsal rhizotomy (SDR) from Jan. 2020 to May. 2021 were retrospectively reviewed. Results Fifty-seven cases with spastic CP were included in the study. Among these cases, 46 were ambulatory and 11 were non-ambulatory. Average gross motor function measure - 66 (GMFM - 66) score was 62.16 ± 11.39. Reduced bladder capacity was seen in 49.12% of these cases and cases with lower GMFM - 66 score had a higher incidence rate of having low bladder capacity (p < 0.01). Detrusor overactivity (DO) was shown in 33.33% of patients. Cases with younger age had higher prevalence of DO (p < 0.05). Meanwhile, more non-ambulant patients had DO (p < 0.05). Increased post-voiding residual (PVR) was seen in 21.05% of cases. Those with higher average threshold in sphincter-associated input spinal nerve roots (rootlets) had higher rate of having abnormal PVR (p < 0.05). Conclusion Abnormal UDS results were prevalent in pediatric spastic CP. Motor function, age and threshold of their sphincter-associated spinal nerve rootlets were related to the abnormal UDS results.
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- 2021
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22. Lnc-RP11-536 K7.3/SOX2/HIF-1α signaling axis regulates oxaliplatin resistance in patient-derived colorectal cancer organoids
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Xinxiang Li, Jing Li, Ziliang Wang, Peiyong Zheng, Shaobo Mo, Yan Li, Lei Liang, Lu Gan, Midie Xu, Qingguo Li, Dakui Luo, Yufei Yang, Huizhen Sun, and Weixing Dai
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Male ,Organoid ,Cancer Research ,Carcinogenesis ,Colorectal cancer ,Angiogenesis ,Antineoplastic Agents ,In situ hybridization ,Biology ,SOX2 ,In vivo ,medicine ,Humans ,RC254-282 ,Research ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,medicine.disease ,Colon cancer ,Oxaliplatin ,Organoids ,Lnc-RP11-536 K7.3 ,Oncology ,Drug Resistance, Neoplasm ,Apoptosis ,Cancer research ,Female ,RNA, Long Noncoding ,Colorectal Neoplasms ,Signal Transduction ,medicine.drug - Abstract
Background Resistance to oxaliplatin is a major obstacle for the management of locally advanced and metastatic colon cancer (CC). Although long noncoding RNAs (lncRNAs) play key roles in CC, the relationships between lncRNAs and resistance to oxaliplatin have been poorly understood yet. Methods Chemo-sensitive and chemo-resistant organoids were established from colon cancer tissues of the oxaliplatin-sensitive or -resistant patients. Analysis of the patient cohort indicated that lnc-RP11-536 K7.3 had a potential oncogenic role in CC. Further, a series of functional in vitro and in vivo experiments were conducted to assess the effects of lnc-RP11-536 K7.3 on CC proliferation, glycolysis, and angiogenesis. RNA pull-down assay, luciferase reporter and fluorescent in situ hybridization assays were used to confirm the interactions between lnc-RP11-536 K7.3, SOX2 and their downstream target HIF-1α. Results In this study, we identified a novel lncRNA, lnc-RP11-536 K7.3, was associated with resistance to oxaliplatin and predicted a poor survival. Knockout of lnc-RP11-536 K7.3 inhibited the proliferation, glycolysis, and angiogenesis, whereas enhanced chemosensitivity in chemo-resistant organoids and CC cells both in vitro and in vivo. Furthermore, we found that lnc-RP11-536 K7.3 recruited SOX2 to transcriptionally activate USP7 mRNA expression. The accumulative USP7 resulted in deubiquitylation and stabilization of HIF-1α, thereby facilitating resistance to oxaliplatin. Conclusion In conclusion, our findings indicated that lnc-RP11-536 K7.3 could promote proliferation, glycolysis, angiogenesis, and chemo-resistance in CC by SOX2/USP7/HIF-1α signaling axis. This revealed a new insight into how lncRNA could regulate chemosensitivity and provide a potential therapeutic target for reversing resistance to oxaliplatin in the management of CC.
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- 2021
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23. OCT4 Promotes Ovarian Cancer Cell Metastasis and Angiogenesis via Modulating VEGFR2/LRPPRC Pathway
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Weiwei Xie, Huizhen Sun, Husheng Wang, Dongling Wu, Xin Chen, and Wei Chen
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genetic structures ,biology ,Angiogenesis ,business.industry ,VEGF receptors ,Cell ,medicine.disease ,Metastasis ,medicine.anatomical_structure ,embryonic structures ,Cancer research ,medicine ,biology.protein ,Ovarian cancer ,business - Abstract
Background: Due to its high ability of metastasis, ovarian cancer remains the most lethal gynecological malignancy, yet its underlying mechanism remains unconfirmed. Objectives: The main purpose is to probe into the role and regulation mechanism of octamer-binding transcription factor 4 (OCT4) in angiogenesis and metastasis in ovarian cancer.Methods: Immunohistochemistry (IHC) and immunofluorescence in epithelial ovarian cancer specimens and benign ovarian tumor samples were performed, followed by RNA-sequencing and examination of angiogenesis, cell migration and invasion in OCT4 knockdown cell lines and the controls. Co-Immunoprecipitation (Co-IP), mass spectrometry, immunoblotting, immunofluorescence and chromatin immunoprecipitation (ChIP) analyses were conducted along with models of zebrafishes and nude mice of transplanted tumors to gain insights into the specific functions and mechanisms of action of OCT4 in ovarian cancer.Results: Firstly, we discovered that OCT4 expression was enhanced in ovarian cancer tissues significantly, especially in the metastatic lesions, indicating that OCT4 might be a key for the metastasis of ovarian cancer. Furtherly, we observed and verified that OCT4 promoted cell migration and invasion, and induced angiogenesis in vitro and in vivo. Mechanistically, OCT4 modulated the transcription of leucine‑rich PPR motif‑containing protein (LRPPRC),and furtherly interacted with vascular endothelial growth factor receptor 2 (VEGFR2)/LRPPRC complex and ultimately triggered the downstream FAK/AKT signaling pathway . Conclusions: Together, through models of ovarian cancer cells, zebrafishes and tumor-transplanted mice, this study highlighted the importance of OCT4/LRPPRC/VEGFR2 signaling axis in metastasis of ovarian cancer and angiogenesis. Thus, our finding supplied a potential novel molecular-targeted approach for the treatment of ovarian cancer.
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- 2021
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24. Ovarian cancer: epigenetics, drug resistance, and progression
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Feikai Lin, Ziliang Wang, Xiaoduan Li, Weiwei Xie, Xipeng Wang, and Huizhen Sun
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Cancer Research ,Review ,medicine.disease_cause ,Ovarian cancer ,microRNA ,Gene expression ,Genetics ,medicine ,Epigenetics ,Gene ,RC254-282 ,DNA methylation ,QH573-671 ,biology ,Histone modifications ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,LncRNA ,Histone ,Oncology ,biology.protein ,Cancer research ,Histone deacetylase ,MiRNA ,Cytology ,Carcinogenesis - Abstract
Ovarian cancer (OC) is one of the most common malignant tumors in women. OC is associated with the activation of oncogenes, the inactivation of tumor suppressor genes, and the activation of abnormal cell signaling pathways. Moreover, epigenetic processes have been found to play an important role in OC tumorigenesis. Epigenetic processes do not change DNA sequences but regulate gene expression through DNA methylation, histone modification, and non-coding RNA. This review comprehensively considers the importance of epigenetics in OC, with a focus on microRNA and long non-coding RNA. These types of RNA are promising molecular markers and therapeutic targets that may support precision medicine in OC. DNA methylation inhibitors and histone deacetylase inhibitors may be useful for such targeting, with a possible novel approach combining these two therapies. Currently, the clinical application of such epigenetic approaches is limited by multiple obstacles, including the heterogeneity of OC, insufficient sample sizes in reported studies, and non-optimized methods for detecting potential tumor markers. Nonetheless, the application of epigenetic approaches to OC patient diagnosis, treatment, and prognosis is a promising area for future clinical investigation.
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- 2021
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25. Abstract P5-08-08: Not presented
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Yancheng Zhao, Wei Jin, and Huizhen Sun
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Oncology ,Cancer Research ,medicine.medical_specialty ,Breast cancer ,business.industry ,Internal medicine ,medicine ,Cancer ,medicine.disease ,business - Abstract
This abstract was not presented at the conference. Citation Format: Zhao Y, Sun H, Jin W. Not presented [abstract]. In: Proceedings of the 2018 San Antonio Breast Cancer Symposium; 2018 Dec 4-8; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2019;79(4 Suppl):Abstract nr P5-08-08.
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- 2019
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26. Dose-response relationships between polycyclic aromatic hydrocarbons exposure and platelet indices
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Huizhen Sun, Jian Hou, Weihong Chen, Chen Hu, Jing Yuan, Chunjie Yuan, and Yun Zhou
- Subjects
Adult ,Blood Platelets ,Male ,Generalized linear model ,China ,medicine.medical_specialty ,Adolescent ,010504 meteorology & atmospheric sciences ,Health, Toxicology and Mutagenesis ,010501 environmental sciences ,Toxicology ,Logistic regression ,01 natural sciences ,Gastroenterology ,Cohort Studies ,Internal medicine ,medicine ,Humans ,Platelet ,Lymphocyte Count ,Polycyclic Aromatic Hydrocarbons ,Mean platelet volume ,Aged ,0105 earth and related environmental sciences ,Aged, 80 and over ,Platelet indices ,business.industry ,Platelet Distribution Width ,Environmental Exposure ,General Medicine ,Baseline data ,Middle Aged ,Pollution ,Restricted cubic splines ,Environmental Pollutants ,Female ,business ,Mean Platelet Volume ,Biomarkers - Abstract
The relations of polycyclic aromatic hydrocarbons (PAHs) exposure with platelet indices remain unclear. Based on the baseline data from the Wuhan-Zhuhai Cohort Study, we used generalized linear model, multivariate logistic regression analysis and restricted cubic splines (RCS) to assess linear and nonlinear relationship of PAHs exposure with platelet indices. The results showed that among Wuhan individuals, there were the non-linear relations between total hydroxynaphthalene (ΣOHNa) and mean platelet volume (MPV) or ratio of mean platelet volume to platelet count (MPVP), total hydrophenanthrene (ΣOHPh) and MPV or platelet distribution width (PDW), the sum concentration of urinary monohydroxylated metabolites of PAHs (ΣOH-PAHs) and ratio of platelet count to lymphocyte count (PLR) or MPVP, 1-hydropyrene (1-OHP) and PLR or PDW. But among Zhuhai individuals, neither linear nor non-linear relations were found between each of OH-PAHs or ΣOH-PAHs and platelet indices. The findings indicate that serum MPV and MPVP may be independent biomarkers of effects of exposing to environmental PAHs on human bodies.
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- 2019
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27. Phloridzin Reveals New Treatment Strategies for Liver Fibrosis
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Yahong Shi, Tun Yan, Xi Lu, Kai Li, Yifeng Nie, Chuqiao Jiao, Huizhen Sun, Tingting Li, Xiang Li, and Dong Han
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Drug Discovery ,Pharmaceutical Science ,Molecular Medicine ,phloridzin ,liver fibrosis ,mRNA ,lncRNA ,ferroptosis ,energy metabolism ,biomechanics - Abstract
Liver fibrosis is an urgent public health problem which is difficult to resolve. However, various drugs for the treatment of liver fibrosis in clinical practice have their own problems during use. In this study, we used phloridzin to treat hepatic fibrosis in the CCl4-induced C57/BL6N mouse model, which was extracted from lychee core, a traditional Chinese medicine. The therapeutic effect was evaluated by biochemical index detections and ultrasound detection. Furthermore, in order to determine the mechanism of phloridzin in the treatment of liver fibrosis, we performed high-throughput sequencing of mRNA and lncRNA in different groups of liver tissues. The results showed that compared with the model group, the phloridzin-treated groups revealed a significant decrease in collagen deposition and decreased levels of serum alanine aminotransferase, aspartate aminotransferase, laminin, and hyaluronic acid. GO and KEGG pathway enrichment analysis of the differential mRNAs was performed and revealed that phloridzin mainly affects cell ferroptosis. Gene co-expression analysis showed that the target genes of lncRNA were obvious in cell components such as focal adhesions, intercellular adhesion, and cell–substrate junctions and in metabolic pathways such as carbon metabolism. These results showed that phloridizin can effectively treat liver fibrosis, and the mechanism may involve ferroptosis, carbon metabolism, and related changes in biomechanics.
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- 2022
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28. Additional file 1 of Seroprevalence of human T-lymphotropic virus infection among blood donors in China: a first nationwide survey
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Chang, Le, Shanhai Ou, Zhengang Shan, Faming Zhu, Huimin Ji, Rong, Xia, Guo, Fei, Xinyi Jiang, Huizhen Sun, Yan, Ying, and Wang, Lunan
- Abstract
Additional file 1: Table S1. Repeatedly reactive donations (n=63) by confirmatory test
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- 2021
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29. Anlotinib plus letrozole in patients with platinum-resistant recurrent ovarian cancer: A prospective, single-arm, open-label, phase II study
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Xipeng Wang, Jiarui Li, Ping Zhang, Xuhong Fang, Yizhi Wang, and Huizhen Sun
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Cancer Research ,Oncology - Abstract
e17545 Background: Studies have suggested that estrogen regulates the malignant transformation of ovarian surface epithelial cells, stimulates the growth and migration of cancer cells. and promotes the VEGF expression in epithelial ovarian cancer. Anlotinib is a novel multi-target tyrosine kinase inhibitor, inhibiting tumor angiogenesis and proliferative signaling. In this study, we evaluate the activity of anlotinib combined with letrozole in patients with estrogen receptor positive, platinum-resistant recurrent ovarian carcinoma (NCT04720807). Methods: Patients who have previously received two or more chemotherapy treatments, with histopathologically confirmed high-grade serous ovarian cancer (including salpingo carcinoma and peritoneal carcinoma), estrogen receptor (ER) positive and ECOG 0-2 were considered eligible for enrollment. Anlotinib was taken orally (10mg mg qd, d1-14, 21 days per cycle), and letrozole was taken orally (2.5mg qd, d1-21, 21 days per cycle). The treatment was continued until disease progression, death, or intolerant toxicity. The primary endpoint was objective response rate (ORR) and the secondary endpoints included disease control rate (DCR), duration of remission (DOR) progression free survival (PFS), overall survival (OS) and safety. Results: Between September 2020 and November 2021, 13 patients with a median age of 62 years (range:53-72), FIGO histopathological stage IC (23.1%), IIIC (53.8%) and IV (23.1%) were enrolled. Twelve of these patients have been evaluated for efficacy. In the efficacy-evaluable population (n=12), the therapeutic evaluation showed that 2 and 7 patients achieved partial response and Stable disease respectively, yielding the ORR of 16.7% (2/12, 95% CI: 3.3 to 54.3). The DCR was 75% (9/12, 95% CI: 39.3 to 93.3). The median PFS was 6.25 months (95% CI: 2.3m to not reached) and the median OS was not reached. The most common adverse events (AEs) were grade 1 or 2, which included laryngitis (69.2%), hypertension (53.8%), stomatitis (38.5%), diarrhea (30.8%), hand-foot syndrome (23.1%), and hyperuricemia (23.1%). The grade 3 AEs were hypertension (38.5%), diarrhea (7.7%) and hyperuricemia (7.7%). No higher AEs or treatment-related death were observed. Conclusions: Conclusions Anlotinib plus letrozole showed a promising efficacy with a favorable toxicity profile for patients with platinum-resistant recurrent ovarian cancer. The trial is ongoing and more data will be provided in the future. Clinical trial information: NCT04720807.
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- 2022
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30. Improving effect of cordycepin on insulin synthesis and secretion in normal and oxidative-damaged INS-1 cells
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Huizhen Sun, Anyong Zhang, Yanchun Gong, Wei Sun, Baiyi Yan, Shuihong Lei, and Li-Hua Yao
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Pharmacology ,Oxidative Stress ,Glucose ,Deoxyadenosines ,Insulin-Secreting Cells ,Insulin ,Hydrogen Peroxide - Abstract
Diabetes mellitus (DM) has recently become one of the major diseases that have received attention. Cordycepin (molecular formula: C
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- 2022
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31. Seroprevalence of human T-lymphotropic virus infection among blood donors in China: a first nationwide survey
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Zhengang Shan, Le Chang, Xinyi Jiang, Shanhai Ou, Huimin Ji, Huizhen Sun, Faming Zhu, Lunan Wang, Fei Guo, Xia Rong, and Ying Yan
- Subjects
lcsh:Immunologic diseases. Allergy ,Adult ,Male ,China ,Blood donor ,Adolescent ,viruses ,Seroprevalence ,Blood Donors ,Human T-lymphotropic virus ,Virus ,Serology ,03 medical and health sciences ,Young Adult ,Sex Factors ,immune system diseases ,Risk Factors ,Seroepidemiologic Studies ,Virology ,Prevalence ,Medicine ,Humans ,Seroconversion ,Risk factor ,030304 developmental biology ,0303 health sciences ,Human T-lymphotropic virus 1 ,biology ,030306 microbiology ,business.industry ,Research ,Human T-lymphotropic virus 2 ,virus diseases ,Middle Aged ,biology.organism_classification ,HTLV-I Infections ,HTLV-I Antibodies ,HTLV-II Antibodies ,Infectious Diseases ,biology.protein ,Female ,Antibody ,business ,lcsh:RC581-607 - Abstract
Background So far, the prevalence of human T-lymphotropic virus (HTLV) type 1 and 2 in some highly populated countries such as China is still unknown. In this study, a multi-center nationwide serological survey was designed and performed, to reveal the seroprevalence of HTLV infection among Chinese blood donors. Results Among 8,411,469 blood donors from 155 blood establishments, 435 were finally confirmed as HTLV carriers. The prevalence of HTLV infection in China varied in different provinces: Fujian had the highest prevalence of 36.240/100,000 (95% CI 31.990–41.050) and eleven provinces did not find HTLV-seropositive donors in the three years. no HTLV-2 infection was found. The overall prevalence of HTLV-1 in China decreased from 2016 to 2018. Female was identified as an independent risk factor of HTLV infection in China. Besides, seroconversion was observed in two of seven seroindeterminate donors 85 and 250 days after their last donation, respectively. Conclusions The seroprevalence of HTLV infection in most areas of China among blood donors is quite low, but it varies significantly in different geographic areas. Screening anti-HTLV-1/2 antibody and follow-up of serointederminate donors are essential to ensure blood safety especially in areas where we have found HTLV infected donors.
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- 2020
32. Hepatitis B virus pre-S region: Clinical implications and applications
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Le Chang, Ying Yan, Huizhen Sun, and Lunan Wang
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0301 basic medicine ,Hepatitis B virus ,Cirrhosis ,business.industry ,030106 microbiology ,Family Hepadnaviridae ,DNA virus ,medicine.disease_cause ,medicine.disease ,Virology ,Genome ,digestive system diseases ,03 medical and health sciences ,030104 developmental biology ,Infectious Diseases ,Viral life cycle ,Hepatocellular carcinoma ,Medicine ,business ,Fulminant hepatitis - Abstract
Hepatitis B virus (HBV) infection is a major threat to global public health, which can result in many acute and chronic liver diseases. HBV, a member of the family Hepadnaviridae, is a small enveloped DNA virus containing a circular genome of 3.2 kb. Located upstream of the S-open-reading frame of the HBV genome is the pre-S region, which is vital to the viral life cycle. The pre-S region has high variability and many mutations in the pre-S region are associated with several liver diseases, such as fulminant hepatitis (FH), liver cirrhosis (LC), and hepatocellular carcinoma (HCC). In addition, the pre-S region has been applied in the development of several pre-S-based materials and systems to prevent or treat HBV infection. In conclusion, the pre-S region plays an essential role in the occurrence, diagnosis, and treatment of HBV-related liver diseases, which may provide a novel perspective for the study of HBV infection and relevant diseases.
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- 2020
33. The clinical and epidemiological features and hints of 82 confirmed COVID-19 pediatric cases aged 0-16 in Wuhan, China
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Haijing Yu, Cai Q, Xuezhen Liu, Xiayun Dai, and Huizhen Sun
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Pediatrics ,medicine.medical_specialty ,Infection sources ,Coronavirus disease 2019 (COVID-19) ,business.industry ,Epidemiology ,Medicine ,China ,business - Abstract
Although COVID-19 pediatric patients just account for 1% of the overall cases, they are nonnegligible invisible infection sources. We quantitatively analyzed the clinical and epidemiological features of 82 confirmed cases aged 0-16 admitted to Wuhan Children’s Hospital, which are expected to shed some lights onto the pediatric diagnosis and therapy.
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- 2020
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34. Development of a nomogram to predict prognosis in ovarian cancer: a SEER-based study
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Hainan Chen, Tao Zheng, Yi Zhang, Huizhen Sun, Husheng Wang, and Li Yan
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Oncology ,Cancer Research ,medicine.medical_specialty ,business.industry ,Nomogram ,medicine.disease ,nomogram ,Ovarian cancer ,Internal medicine ,medicine ,risk factors ,Radiology, Nuclear Medicine and imaging ,Original Article ,prognosis ,business - Abstract
Background Ovarian cancer remains the most lethal gynecologic malignancy. In this study, we aimed to identify the specific risk factors affecting overall survival (OS) and develop a nomogram for prognostic prediction of ovarian cancer patients based on data from the Surveillance, Epidemiology, and End Results (SEER) database. Methods Information from the SEER database on ovarian cancer between 2004 and 2016 was screened and retrieved. Cases were randomly divided into the training cohort hand the validation cohort at a 7:3 ratio. The prognostic effects of individual variables on survival were evaluated via Kaplan-Meier method and Cox proportional hazards regression model using data from the training cohort. A nomogram was formulated to predict the 3- and 5-year OS rates of patients with ovarian cancer, and then validated both in the training cohort and the validation cohort. Results A total of 28,375 patients were selected from 75,921 samples (19,862 in training cohort and 8,513 in validation cohort). Cox regression analysis identified race, age laterality, histology, stage, grade, surgery, chemotherapy, radiotherapy, and marital status as independent risk factors for ovarian cancer prognosis. A nomogram was developed based on the results of multivariate analysis and validated using an internal bootstrap resampling approach, which demonstrated a sufficient level of discrimination according to the C-index (0.752, 95% CI: 0.746–0.758 in the training cohort, 0.755, 95% CI: 0.746–0.764). Conclusions We developed a nomogram valuable for accurate prediction of 3- and 5-year OS rates of ovarian cancer patients based on individual characteristics.
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- 2020
35. Preparation of gelatin-based films modified with nanocrystalline cellulose
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Haichao Li, Shuaishuai Yang, and Huizhen Sun
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Materials science ,food.ingredient ,Absorption of water ,Polymers and Plastics ,General Chemical Engineering ,Composite number ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Gelatin ,chemistry.chemical_compound ,food ,parasitic diseases ,Ultimate tensile strength ,Materials Chemistry ,Thermal stability ,Cellulose ,urogenital system ,Plasticizer ,021001 nanoscience & nanotechnology ,Nanocrystalline material ,0104 chemical sciences ,Chemical engineering ,chemistry ,embryonic structures ,0210 nano-technology - Abstract
Gelatin is a natural biological macromolecule derived from the collagen in the connective tissue of the skin, bone and other tissues. It has been widely used in medicine, food and industrial production and other fields for easy molding, excellent compatibility and biodegradability. However, physical and chemical disadvantages impede its further application, seriously. Therefore, modification of the gelatin films becomes more and more important. In this study, the gelatin/nanocrystalline cellulose (NCC) composite films were prepared by casting method with 4% glycerol as plasticizer. The effect of NCC on the properties of the composite films was investigated by the characterization of its morphology and mechanical, thermal, and optical properties and water adsorption. The results showed that mechanical, thermal stability and water absorption properties of the gelatin/NCC composite film were obviously improved. The composite films showed the highest tensile strength (13.56 ± 0.25 MPa) when the mass concentration of NCC was 0.6%. Adding NCC to gelatin benefited the thermal stability of composite films. The gelatin/NCC composite film of 0.4% NCC had the highest melting transition temperature (138.9 °C). The composite films exhibited the lower water absorption (271.1%) when mass concentration of NCC was 1.0%. Thus, these results indicated that NCC could affect the properties of gelatin-based composite films, and showed it has potential for application in food packing.
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- 2018
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36. New design of probe and central-homo primer pairs to improve TaqMan™ PCR accuracy for HBV detection
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Shang He, Huizhen Sun, Shanshan Liu, Xiaoting Liu, Fan Zhang, Chengbin Wang, Chen Chen, Hong Jiang, Jianan Wang, Wencan Jiang, Hongli Tong, Mianyang Li, and Suwen Yue
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Male ,0301 basic medicine ,Hepatitis B virus ,Reproducibility of Results ,Computational biology ,Biology ,Hepatitis B ,Real-Time Polymerase Chain Reaction ,Sensitivity and Specificity ,Homologous Sequences ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Real-time polymerase chain reaction ,Duplex (building) ,Molecular Probes ,030220 oncology & carcinogenesis ,Virology ,Primer dimer ,Nucleic acid ,TaqMan ,Humans ,Female ,Primer (molecular biology) ,DNA Primers - Abstract
Quantitative PCR (qPCR) assay using TaqMan™ probe was widely used in the detection of different nucleic acids. However, this technology has several drawbacks, including false negative results caused by primer-dimer (PD) and false positive issues due to primer-probe aggregations. Here, we designed a modified TaqMan™-Molecular Beacon probe by adding an antisense base and a new type of primer pair named central-homo primer pairs bearing 5-10 bases homologous sequence on the 3' end. Using the HBV qPCR assay as a proof of concept, the new design significantly improved the accuracy of the TaqMan™ qPCR assay for HBV detection. Application of the central-homo primer pair led to significantly delayed Ct values by 5-10 cycles compared with conventional primer design. The modified probe containing an antisense base did not produce any detectable signal in repeating primer-probe aggregation experiments. Furthermore, the use of the central-homo primer pair and the non-competitive internal control could solve the false negative problem caused by PD formation. We validated this customized duplex qPCR system using 208 clinical samples collected from patients in clinic showing accuracy was higher than that of the conventional qPCR method.
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- 2018
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37. Associations of urinary polycyclic aromatic hydrocarbon metabolites with fractional exhaled nitric oxide and exhaled carbon monoxide: A cross-sectional study
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Lili Xiao, Yun Zhou, Jing Yuan, Huizhen Sun, Wei Li, Bin Wang, Weihong Chen, Jixuan Ma, Yuewei Liu, and Limin Cao
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Adult ,Male ,China ,medicine.medical_specialty ,Environmental Engineering ,Cross-sectional study ,Urinary system ,Inflammatory response ,Polycyclic aromatic hydrocarbon ,010501 environmental sciences ,Nitric Oxide ,01 natural sciences ,Gastroenterology ,Nitric oxide ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Internal medicine ,medicine ,Humans ,Environmental Chemistry ,Polycyclic Aromatic Hydrocarbons ,Waste Management and Disposal ,Aged ,0105 earth and related environmental sciences ,chemistry.chemical_classification ,Carbon Monoxide ,business.industry ,Smoking ,Middle Aged ,Pollution ,Confidence interval ,respiratory tract diseases ,Cross-Sectional Studies ,Breath Tests ,030228 respiratory system ,chemistry ,Exhalation ,Exhaled nitric oxide ,Female ,business ,Biomarkers ,Carbon monoxide - Abstract
Exposure to Polycyclic aromatic hydrocarbons (PAHs) has been associated with inflammatory responses. Fractional exhaled nitric oxide (FeNO) and exhaled carbon monoxide (eCO) are both important inflammatory mediators especially in airways. However, few studies have investigated associations of PAH exposures with FeNO or eCO. Therefore, we aimed to quantify the associations of urinary PAH metabolites with FeNO and eCO levels, and investigate their potential effect modifiers by linear mixed models among 4133 participants from the Wuhan-Zhuhai cohort in China. We further performed stratified analyses to estimate effect modification. We found significant associations of increased urinary PAH metabolites with elevated eCO and FeNO. Among all participants, each 1% increase of 1-hydroxynaphthalene, 2-hydroxynaphthalene, 2-hydroxyfluorene, 4-hydroxyphenanthrene, 3-hydroxyphenanthrene, and total PAH metabolites was significantly associated with a 12.6% (95% confidence interval: 9.3%, 15.9%), 9.7% (6.5%, 12.9%), 7.5% (4.1%, 10.9%), 3.2% (0.2%, 6.2%), 2.7% (0.1%, 5.3%), and 6.5% (2.7%, 10.4%) increased eCO level, respectively; while each 1% increase of urinary 1-hydroxynaphthalene, 9-hydroxyphenanthrene, 3-hydroxyphenanthrene, and 2-hydroxyphenanthrene was associated with a -3.0% (-5.8%, -0.2%), 2.9% (0.3%, 5.6%), 3.2% (1.0%, 5.4%), and 4.5% (2.2%, 6.9%) change of FeNO level, respectively. Positive associations between certain urinary PAH metabolites and eCO were observed among both ever-smokers and non-smokers, and the associations were stronger among ever-smokers than that among non-smokers. Increased urinary PAH metabolites were associated with decreased FeNO among ever-smokers and elevated FeNO levels among non-smokers. Our findings suggest that PAH exposures may impair airway through inducing inflammatory response, especially among ever-smokers.
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- 2018
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38. Association of polycyclic aromatic hydrocarbons exposure with atherosclerotic cardiovascular disease risk: A role of mean platelet volume or club cell secretory protein
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Wenjun Yin, Xian Wang, Jing Yuan, Chen Hu, Guiyang Wang, Huizhen Sun, Jian Hou, Weihong Chen, Youjian Zhang, and Yun Zhou
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Male ,China ,medicine.medical_specialty ,Health, Toxicology and Mutagenesis ,Urinary system ,Physical examination ,030204 cardiovascular system & hematology ,010501 environmental sciences ,Toxicology ,Logistic regression ,01 natural sciences ,Gastroenterology ,Gas Chromatography-Mass Spectrometry ,Cohort Studies ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,medicine ,Humans ,Polycyclic Aromatic Hydrocarbons ,Mean platelet volume ,0105 earth and related environmental sciences ,Inflammation ,medicine.diagnostic_test ,Chemistry ,Environmental Exposure ,General Medicine ,Environmental exposure ,Middle Aged ,Phenanthrenes ,Pollution ,Logistic Models ,Club cell ,Secretory protein ,Biochemistry ,Cardiovascular Diseases ,Female ,Mean Platelet Volume ,Cohort study - Abstract
Background Inflammation may play an important role in the association between exposure to polycyclic aromatic hydrocarbons (PAHs) and atherosclerotic cardiovascular disease (ASCVD) risk. However, the underlying mechanisms remain unclear. Objectives To investigate the association of PAHs exposure with ASCVD risk and effects of mean platelet volume (MPV) or Club cell secretory protein (CC16) on the association. Methods A total of 2022 subjects (689 men and 1333 women) were drawn from the baseline Wuhan residents of the Wuhan-Zhuhai Cohort study. Data on demography and the physical examination were obtained from each participant. Urinary monohydroxy PAH metabolites (OH-PAHs) levels were measured by a gas chromatography-mass spectrometry. We estimated the association between each OH-PAHs and the 10-year ASCVD risk or coronary heart disease (CHD) risk using logistic regression models, and further analyze the mediating effect of MPV or plasma CC16 on the association by using structural equation modeling. Results The results of multiple logistic regression models showed that some OH-PAHs were positively associated with ASCVD risk but not CHD risk, including 2-hydroxyfluoren (β = 1.761; 95% CI: 1.194–2.597), 9-hydroxyfluoren (β = 1.470; 95% CI: 1.139–1.898), 1-hydroxyphenanthrene (β = 1.480; 95% CI: 1.008–2.175) and ΣOH-PAHs levels (β = 1.699; 95% CI: 1.151–2.507). The analysis of structural equation modeling shows that increased MPV and increased plasma CC16 levels contributed 13.6% and 15.1%, respectively, to the association between PAHs exposure and the 10-year ASCVD risk (p Conclusions Exposure to PAHs may increase the risk of atherosclerosis, which was partially mediated by MPV or CC16.
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- 2018
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39. Spectrophotometric determination of chlorophylls in different solvents related to the leaf traits of the main tree species in Northeast China
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Shanshan Liu, Huizhen Sun, Kangkang Chen, and Gaiyan Li
- Subjects
Chemistry ,Botany ,China ,Tree species - Abstract
The accurate detection of the leaf chlorophyll (Chl) is of substantial importance for the immediate assessment of forest conditions to manage and conserve forest ecosystems. We compared 80% acetone, 95% ethanol, and dimethyl sulfoxide (DMSO) over a range of incubation times (2, 4, 6, 8, 18, 26, and 32 h) to determine the Chl contents of 12 tree species in northeast China. The results showed that to obtain the maximum Chl (a+b) contents for most tree species extracted by 80% acetone and 95% ethanol required a minimum of 18 h, while the incubation periods by DMSO were 2-6 h and 18-32 h to extract 90% of the Chl from the broadleaved and coniferous tree species, respectively. We observed that the amount of Chl extracted with DMSO was significantly higher than that extraction with 80% acetone and 95% ethanol, particularly for conifer species with the exception of Phellodendron amurense, Fraxinus mandshurica, and Tilia amurensis, in which the maximum amount of Chl was extracted with acetone. The DMSO extracted Chl in exhibited the lowest degree of variation among the three solvents. The leaf mass area (LMA), leaf thickness, and diameter of the primary leaf vein were significantly negatively correlated with the Chl a, Chl b, and Chl (a+b) content for the 12 tree species. There were non-significant different slopes or intercepts between the curves for LMA and Chl a, Chl b, or Chl (a+b) at the different incubation times for the same solvent or the different solvents at the certain incubation time (P > 0.05).
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- 2021
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40. Dose-response relationship between urinary polycyclic aromatic hydrocarbons metabolites and urinary 8-hydroxy-2′-deoxyguanosine in a Chinese general population
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Tian Xu, Juan Cheng, Huizhen Sun, Jian Hou, Lili Xiao, Yuqing Yang, Yun Zhou, Weihong Chen, and Jing Yuan
- Subjects
Adult ,Male ,0301 basic medicine ,Environmental Engineering ,Health, Toxicology and Mutagenesis ,Urinary system ,Population ,Physiology ,010501 environmental sciences ,01 natural sciences ,Gas Chromatography-Mass Spectrometry ,Cohort Studies ,03 medical and health sciences ,Asian People ,Humans ,Environmental Chemistry ,Obesity ,Polycyclic Aromatic Hydrocarbons ,education ,Chromatography, High Pressure Liquid ,Aged ,0105 earth and related environmental sciences ,education.field_of_study ,Dose-Response Relationship, Drug ,Chemistry ,Public Health, Environmental and Occupational Health ,Deoxyguanosine ,8-Hydroxy-2'-deoxyguanosine ,General Medicine ,General Chemistry ,Middle Aged ,Pollution ,Dose–response relationship ,030104 developmental biology ,8-Hydroxy-2'-Deoxyguanosine ,Environmental chemistry ,Environmental Pollutants ,Female ,Multiple linear regression analysis ,Biomarkers ,Environmental Monitoring ,Cohort study - Abstract
Association of exposure to polycyclic aromatic hydrocarbons (PAHs) with increased urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) formation has been reported in occupational population and children. However, studies on the association between them in general population are limited. A total of 1864 eligible subjects from the baseline Wuhan participants of the Wuhan-Zhuhai Cohort Study (n = 3053) were included in this study, after excluding individuals with certain disease and missing data on urinary monohydroxy PAHs (OH-PAHs) and 8-OHdG levels. Urinary monohydroxy PAHs and 8-OHdG levels were measured by gas chromatography-mass spectrometry and high performance liquid chromatography-electrochemical detection, respectively. Association of urinary OH-PAHs with urinary 8-OHdG was analyzed by multiple linear regression analysis. We found a dose-dependent relationship between urinary PAHs metabolites and urinary 8-OHdG (p 0.05 for all). Furthermore, more evidence for the association of total concentrations of urinary OH-PAHs with 8-OHdG levels were observed in individuals with normal body mass index or central obesity (p 0.01 for all). There was a dose-dependent relationship between urinary OH-PAHs levels and urinary 8-OHdG levels among a general Chinese population. Exposure to background PAHs may have a greater influence on urinary 8-OHdG levels in individuals with central obesity.
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- 2017
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41. Highly expressed NRSN2 is related to malignant phenotype in ovarian cancer
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Bin Li, Huizhen Sun, Wenbin Tang, Hongyang Xiao, and Aimin Ren
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0301 basic medicine ,MAPK/ERK pathway ,Oncology ,endocrine system diseases ,Gene Dosage ,Stem cell marker ,0302 clinical medicine ,Cell Movement ,AC133 Antigen ,Extracellular Signal-Regulated MAP Kinases ,Ovarian Neoplasms ,Wnt signaling pathway ,Nuclear Proteins ,General Medicine ,Middle Aged ,Up-Regulation ,Gene Expression Regulation, Neoplastic ,Phenotype ,030220 oncology & carcinogenesis ,Neoplastic Stem Cells ,Female ,RNA Interference ,Signal Transduction ,medicine.medical_specialty ,Paclitaxel ,Antineoplastic Agents ,Adenocarcinoma ,Biology ,Transfection ,03 medical and health sciences ,Cell Line, Tumor ,Internal medicine ,Biomarkers, Tumor ,medicine ,Humans ,Neoplasm Invasiveness ,Protein kinase B ,Cell Proliferation ,Malignant phenotype ,Pharmacology ,Dose-Response Relationship, Drug ,Twist-Related Protein 1 ,Membrane Proteins ,medicine.disease ,030104 developmental biology ,Membrane protein ,Drug Resistance, Neoplasm ,Cancer research ,Cisplatin ,Ovarian cancer ,Proto-Oncogene Proteins c-akt ,Function (biology) - Abstract
Neurensin-2 (NRSN2) is a 24KD protein, which is reported located in the membrane, while its biological functions remain unknown, not to mention in the field of tumor biology. In current study, we aimed to analyze the functions of NRSN2 in ovarian cancer. We screened TCGA database and surprisingly found that its copy number and mRNA level are gained and heightened respectively in parts of serous ovarian cancer patients. In current study, both loss- and gain- function assays found that NRSN2 is associated with the malignant phenotype in ovarian cancer cells, because NRSN2 plays a remarkable role in anchorage-independent colony formation, subcutaneous tumor formation, cell invasion, and chemoresistance. Furthermore, we found that the level of NRSN2 was positively correlated with the expression of stem cell marker CD133. In addition, Wnt canonical signaling and Twist/Akt/Erk axis were also regulated by NRSN2. In conclusion, we found that a poorly studied protein, NRSN2, which is associated with the malignant phenotype of serous ovarian cancer and as a membrane protein; it could be a target for serous ovarian cancer treatment.
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- 2017
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42. Impacts of low socioeconomic status and polycyclic aromatic hydrocarbons exposure on lung function among a community-based Chinese population
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Jing Yuan, Jian Hou, Huizhen Sun, Weihong Chen, Jixuan Ma, Yun Zhou, Juan Cheng, Lili Xiao, and Tian Xu
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China ,Vital capacity ,medicine.medical_specialty ,Environmental Engineering ,Urinary system ,Vital Capacity ,Urine ,010501 environmental sciences ,01 natural sciences ,Cohort Studies ,03 medical and health sciences ,FEV1/FVC ratio ,0302 clinical medicine ,Forced Expiratory Volume ,Environmental health ,medicine ,Humans ,Environmental Chemistry ,030212 general & internal medicine ,Polycyclic Aromatic Hydrocarbons ,Waste Management and Disposal ,Socioeconomic status ,Lung function ,0105 earth and related environmental sciences ,Inhalation exposure ,Inhalation Exposure ,Chemistry ,Pollution ,Surgery ,Social Class ,Cohort study - Abstract
Lung function is related to socioeconomic status (SES) and exposure to polycyclic aromatic hydrocarbons (PAHs). However, joint effect of SES and exposure to PAHs on lung function has been largely unknown. We aimed to investigate joint effects of SES and urinary OH-PAHs levels on lung function parameters. This study included 2739 Wuhan participants from the baseline survey of the Wuhan-Zhuhai (WHZH) Cohort Study (n=3053). They completed the questionnaire, physical examination and provided blood and urine samples. Twelve urinary monohydroxy-PAHs metabolites (OH-PAHs) and lung function were measured by gas chromatography-mass spectrometry and digital spirometers, respectively. Individuals with low educational levels and low or high levels of urinary ΣOH-PAHs had a 3.5% (95% CI: -5.4, -1.6%) or 4.2% (95% CI: -6.1, -2.3%) reduction in the ratio of forced expiratory volume in 1s to forced vital capacity (FEV1/FVC), respectively, and those with middle levels of education and high levels of urinary ΣOH-PAHs had a 2.1% (95% CI: -5.4, -1.6%) reduction in the FEV1/FVC ratio, rather than those with high levels of education and low levels of urinary ΣOH-PAHs. Individuals with low levels of education had a -3.0% (95% CI: -4.4, -1.6%) reduction in FEV1/FVC compared with individuals with high levels of education. Urinary OH-PAHs levels were marginally negatively related to FEV1 in all participants (p=0.073). The results indicated that there was a prominent effect of low levels of education and higher exposure to PAHs on lung function decline, indicating that it is a necessary to take measures to promote the education level and reduce exposure to environmental PAHs.
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- 2017
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43. Abstract P1-03-09: Not presented
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Min Chen, Huizhen Sun, Yupei Zhao, and Wei Jin
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Cancer Research ,Oncology - Abstract
This abstract was not presented at the symposium.
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- 2018
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44. De novo truncating variant in NSD2gene leading to atypical Wolf-Hirschhorn syndrome phenotype
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Guanghai Wang, Jian Wang, Huizhen Sun, Ruen Yao, Qingmin Lin, Fan Jiang, Zengge Wang, and Yanrui Jiang
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0301 basic medicine ,Male ,lcsh:Internal medicine ,medicine.medical_specialty ,Clinodactyly ,lcsh:QH426-470 ,Developmental Disabilities ,NSD2 gene ,Case Report ,030105 genetics & heredity ,Contiguous gene syndrome ,03 medical and health sciences ,Seizures ,Intellectual Disability ,Intellectual disability ,Exome Sequencing ,Genetics ,medicine ,Humans ,Genetic Predisposition to Disease ,lcsh:RC31-1245 ,Child ,Wolf–Hirschhorn syndrome ,Genetics (clinical) ,Exome sequencing ,Wolf-Hirschhorn syndrome ,Base Sequence ,business.industry ,Cytogenetics ,DNA ,Histone-Lysine N-Methyltransferase ,medicine.disease ,Phenotype ,Human genetics ,Repressor Proteins ,lcsh:Genetics ,030104 developmental biology ,Truncating variants ,medicine.symptom ,Chromosomes, Human, Pair 4 ,business - Abstract
Background Wolf-Hirschhorn syndrome (WHS) is a contiguous gene syndrome caused by partial 4p deletion highly variable in size in individual patients. The core WHS phenotype is defined by the association of growth delay, typical facial characteristics, intellectual disability and seizures. The WHS critical region (WHSCR) has been narrowed down and NSD2 falls within this 200 kb region. Only four patients with NSD2 variants have been documented with phenotypic features in detail. Case presentation Herein, we report the case of a 12-year-old boy with developmental delay. He had dysmorphic facial features including wide-spaced eyes, prominent nasal bridge continuing to forehead, abnormal teething and micrognathia. He also had mild clinodactyly of both hands. Using whole-exome sequencing, we identified a pathogenic mutation in NSD2 [c.4029_4030insAA, p.Glu1344Lysfs*49] isolated from peripheral blood DNA. Sanger confirmation of this variant revealed it as a de novo truncating variant in the family. Conclusion Here, we reported a boy with de novo truncating variant in NSD2 with atypical clinical features comparing with 4p16.3 deletion related WHS. Our finding further supported the pathogenesis of truncating variants in NSD2 and delineated the possible symptom spectrum caused by these variants.
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- 2019
45. The Impact of Sharing Primer, the Quantity of the Internal Control Gene and the Primer Dimer on Reaction System in Duplex PCR
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Xiaozhou Yuan, Yingjiao Sha, Shang He, Huizhen Sun, Xiaoting Liu, Jianan Wang, Chengbin Wang, Chen Chen, and Wencan Jiang
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Hepatitis B virus ,Base Sequence ,Chemistry ,Reproducibility of Results ,Duplex (telecommunications) ,Reference Standards ,Hepatitis B ,Real-Time Polymerase Chain Reaction ,Sensitivity and Specificity ,Molecular biology ,General Biochemistry, Genetics and Molecular Biology ,Duplex pcr ,chemistry.chemical_compound ,Plasmid ,Real-time polymerase chain reaction ,Gene Expression Regulation ,Primer dimer ,DNA, Viral ,Humans ,Primer (molecular biology) ,Gene ,DNA ,DNA Primers ,Plasmids - Abstract
Background In duplex real time quantitative PCR (qPCR), there are several factors affecting the sensitivity of duplex qPCR, such as sharing primer, quantity of the internal control (IC) gene, and the primer dimer (PD). The aim of the study is to find out the relationship of interference among templates with different primer pairs, the internal control gene, and the PD in duplex PCR. Methods We designed and synthesized plasmids with partial same sequence and different types of primers include central-homo primer pair, ordinary primer pair, and complementary primer pair. Then we compared the amplification efficiencies of different kinds of primer pairs. Besides, we adjusted the amount of IC plasmid and IC primer to find out the key factor that influences the sensitivity of the target template. Results The concentration ratios of two plasmids showed interference in sharing the universal primer pair, sharing one forward primer, and sharing no primer were 50:1, 200:1 and 500:1, respectively. The amplification efficiency of the ordinary primer pair was higher than that of the universal primer pair for the plasmid. Sensitivity of the duplex qPCR remained unchanged when the amount of PDs increased, but it declined rapidly when the quantity of the IC increased. Conclusions IC is the major factor influencing the sensitivity of the duplex qPCR and it would be better to use one universal primer for IC and target template to achieve minimum interference.
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- 2019
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46. Construction and Photocatalytic Performance of 3D Hierarchical Pore rGO/TiO2 Composites
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Huizhen Sun, Wensheng Li, Wenhui Kong, Cuixia Li, Xiao Shi, and Haize Jin
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Inorganic Chemistry ,Materials science ,Chemical engineering ,Photocatalysis ,Ultraviolet light ,General Materials Science ,Visible spectrum - Published
- 2021
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47. Genome-Wide Analysis of DNA Methylation and Cigarette Smoking in a Chinese Population
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Zhihong Zhang, Dan Kuang, Yansen Bai, Siyun Deng, Liming Liang, Xiaosheng He, Weihong Chen, Kuai Yu, Yizhi Zhang, Bing Liu, Liyun Zhang, Shunchang Sun, Chuanyao Liu, Xiaomin Zhang, Huizhen Sun, Xiaoyan Zhu, Qifei Deng, Xu Han, Xiaoliang Li, Yanjun Guo, Xiaoting Luo, Longxian Cheng, Jing Yuan, Zhiming Zhou, Meian He, Haijing Jiang, Wenhua Mei, Frank B. Hu, Jun Li, Huan Guo, Xuezhen Liu, Tangchun Wu, Liangle Yang, Bing Zhang, and Jie Hu
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Male ,0301 basic medicine ,China ,Health, Toxicology and Mutagenesis ,Genome wide analysis ,Gene Expression ,Genome-wide association study ,Naphthols ,Biology ,03 medical and health sciences ,0302 clinical medicine ,Asian People ,Cigarette smoking ,Risk Factors ,Humans ,Risk factor ,Genetics ,Chinese population ,Research ,Smoking ,Public Health, Environmental and Occupational Health ,Methylation ,DNA Methylation ,030104 developmental biology ,Smoking epidemiology ,030220 oncology & carcinogenesis ,DNA methylation ,Female ,Dinucleoside Phosphates ,Genome-Wide Association Study - Abstract
Background: Smoking is a risk factor for many human diseases. DNA methylation has been related to smoking, but genome-wide methylation data for smoking in Chinese populations is limited. Objectives: We aimed to investigate epigenome-wide methylation in relation to smoking in a Chinese population. Methods: We measured the methylation levels at > 485,000 CpG sites (CpGs) in DNA from leukocytes using a methylation array and conducted a genome-wide meta-analysis of DNA methylation and smoking in a total of 596 Chinese participants. We further evaluated the associations of smoking-related CpGs with internal polycyclic aromatic hydrocarbon (PAH) biomarkers and their correlations with the expression of corresponding genes. Results: We identified 318 CpGs whose methylation levels were associated with smoking at a genome-wide significance level (false discovery rate < 0.05), among which 161 CpGs annotated to 123 genes were not associated with smoking in recent studies of Europeans and African Americans. Of these smoking-related CpGs, methylation levels at 80 CpGs showed significant correlations with the expression of corresponding genes (including RUNX3, IL6R, PTAFR, ANKRD11, CEP135 and CDH23), and methylation at 15 CpGs was significantly associated with urinary 2-hydroxynaphthalene, the most representative internal monohydroxy-PAH biomarker for smoking. Conclusion: We identified DNA methylation markers associated with smoking in a Chinese population, including some markers that were also correlated with gene expression. Exposure to naphthalene, a byproduct of tobacco smoke, may contribute to smoking-related methylation. Citation: Zhu X, Li J, Deng S, Yu K, Liu X, Deng Q, Sun H, Zhang X, He M, Guo H, Chen W, Yuan J, Zhang B, Kuang D, He X, Bai Y, Han X, Liu B, Li X, Yang L, Jiang H, Zhang Y, Hu J, Cheng L, Luo X, Mei W, Zhou Z, Sun S, Zhang L, Liu C, Guo Y, Zhang Z, Hu FB, Liang L, Wu T. 2016. Genome-wide analysis of DNA methylation and cigarette smoking in Chinese. Environ Health Perspect 124:966–973; http://dx.doi.org/10.1289/ehp.1509834
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- 2016
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48. The Effects of Rehydration Time on Pressure-Volume Curves of Larix gmelinii and Fraxinus mandshurica in Northeast China
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Huizhen Sun, Liu Yang, and Robert O. Teskey
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0106 biological sciences ,Larix gmelinii ,Pressure-volume curves ,Horticulture ,Ecology ,biology ,Ecological Modeling ,Forestry ,Fraxinus ,biology.organism_classification ,010603 evolutionary biology ,01 natural sciences ,010606 plant biology & botany - Published
- 2016
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49. Urinary Polycyclic Aromatic Hydrocarbon Metabolites and Altered Lung Function in Wuhan, China
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Xiji Huang, Yuewei Liu, Ting Zhou, Weihong Chen, Yun Zhou, Yi Rong, Tangchun Wu, Yuanchao Song, Jing Yuan, Jungang Xie, and Huizhen Sun
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Lung Diseases ,Male ,0301 basic medicine ,Pulmonary and Respiratory Medicine ,China ,Metabolite ,Urinary system ,Physiology ,Polycyclic aromatic hydrocarbon ,010501 environmental sciences ,Critical Care and Intensive Care Medicine ,01 natural sciences ,Toxicology ,03 medical and health sciences ,chemistry.chemical_compound ,polycyclic compounds ,Humans ,Medicine ,Polycyclic Aromatic Hydrocarbons ,Respiratory system ,Adverse effect ,Lung ,Lung function ,0105 earth and related environmental sciences ,chemistry.chemical_classification ,business.industry ,Middle Aged ,030104 developmental biology ,medicine.anatomical_structure ,chemistry ,Cohort ,Female ,business - Abstract
Exposure to polycyclic aromatic hydrocarbons (PAHs) has been associated with adverse effects on the respiratory system. However, the association between internal levels of PAH metabolites and lung function levels remains unclear.We investigated the relationships between urinary PAH metabolite concentrations and lung function levels in a general Chinese population.Lung function and 12 urinary PAH metabolites were measured in 2,747 participants from the Wuhan-Zhuhai cohort in China. Associations between urinary PAH metabolites and lung function were analyzed by linear mixed models. We also investigated associations among urinary PAH metabolite concentrations, traffic exposure time, and dietary PAH exposure.We found significant associations between increased levels of urinary PAH metabolites and reduced lung function. Each 1-U increase in log-transformed levels of 2-hydroxynaphthalene, 9-hydroxyfluorene, 2-hydroxyfluorene, 4-hydroxyphenanthrene, 9-hydroxyphenanthrene, 3-hydroxyphenanthrene, 1-hydroxyphenanthrene, 2-hydroxyphenanthrene, 1-hydroxypyrene, or total urinary PAH metabolites was associated with a 23.79-, 19.36-, 41.76-, 36.87-, 33.47-, 27.37-, 39.53-, 34.35-, 25.03-, or 37.13-ml reduction in FEV1, respectively (all P 0.05). Each 1-U increase in 2-hydroxynaphthalene, 2-hydroxyfluorene, 4-hydroxyphenanthrene, 1-hydroxyphenanthrene, 2-hydroxyphenanthrene, or total urinary PAH metabolites was associated with a 24.39-, 33.90-, 27.15-, 28.56-, 27.46-, or 27.99-ml reduction in FVC, respectively (all P 0.05). The total urinary PAH metabolites concentration was positively associated with both traffic exposure time and dietary PAH exposure among nonsmokers.Total and specific urinary PAH metabolites were associated with lung function reduction in a general Chinese population. Further studies are needed to investigate the potential mechanism by which PAHs induces lung function reduction.
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- 2016
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50. Molecular characterization of hepatitis C virus for subtype determination and resistance-associated substitutions detection among Chinese voluntary blood donors
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Huimin Ji, Ying Yan, Mary A. Rodgers, Xinyi Jiang, Fei Guo, Xiaoting Lv, Le Chang, Lunan Wang, Huizhen Sun, and Peng Yin
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0301 basic medicine ,China ,Hepatitis C virus ,030106 microbiology ,Mutant ,Mutation, Missense ,Blood Donors ,Hepacivirus ,Disease ,Viral Nonstructural Proteins ,Biology ,medicine.disease_cause ,Antiviral Agents ,03 medical and health sciences ,Asian People ,Virology ,Drug Resistance, Viral ,Prevalence ,medicine ,Humans ,Protease inhibitor (pharmacology) ,NS5A ,Phylogeny ,Pharmacology ,NS3 ,Transition (genetics) ,virus diseases ,Hepatitis C ,digestive system diseases ,030104 developmental biology ,Codon usage bias - Abstract
Background The high prevalence of hepatitis C virus (HCV) infection and the resulting burden of the disease are significant issues to public health worldwide. Although direct-acting antiviral drugs (DAAs) with good tolerance and bioavailability are available, resistance-associated substitutions (RASs) often jeopardize the successful sustainment of virological responses in HCV treatment. High-frequency baseline RASs in treatment-naive patients can lead to failures in DAA treatment. Clinical data on HCV RASs in patients from China are limited and require investigations. Methods 262 HCV RNA positive plasma from Chinese blood donors were genotyped and amplified with subtype-specific primers for NS3 and NS5A regions. RASs were analyzed using Geno2pheno. The codon usage of each resistance-associated substitution was calculated for genetic barrier analysis. Results The two main subtypes in mainland China were 1b and 2a, followed by subtype 6a, 3b, 3a, and 1a. In NS3 region of 1b subtype, substitutions (T54S, V55A, Y56F, Q80 K/L, S122 G/T, R117 H/C, V170I and S174A) were present in 89.7% (96/107) of the samples. Other RASs (M28L, R30Q, P58 L/S and Y93H) were observed in 22.1% (25/113) of the samples in NS5A region. A crucial RAS, Q80K, and two other mutations (S122G + V170I) was identified in the same sequence, which reduced its susceptibility to protease inhibitor ASV and resulted in resistance to SMV. In NS5A, Y93H was detected in 9.7% (11/113) of the 1b samples, leading to medium-to-high level resistance to all six commercialized NS5A inhibitors. S122G-NS3 and Y93H-NS5A occurred simultaneously in 38.1% (7/22) of the samples with mutations in both two regions. Moreover, codon usage of S122G-NS3 and Y93H-NS5A revealed that both variants had the lowest genetic barrier and required only one transition to confer resistance. Conclusions Low genetic barriers facilitated the generation of resistance mutants and threated the efficacy of DAA regimens. The baseline RASs posed a great challenge to real-world DAA application.
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- 2020
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