74 results on '"Ji Yeon Son"'
Search Results
2. A Study on Gangwon-do Type Wellness Tourism Cluster Environment Creation Plan
- Author
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Ji-Yeon Son and Yunho Ji
- Published
- 2022
3. Supplementary Figure 3 from EW-7197, a Novel ALK-5 Kinase Inhibitor, Potently Inhibits Breast to Lung Metastasis
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Yhun Yhong Sheen, Jeong-Seok Nam, Dae-Kee Kim, Seung Won Kim, Min-Jin Kim, Sang-A. Park, Seon Joo Lee, Sol-Ji Kim, So-Yeon Park, and Ji Yeon Son
- Abstract
PDF - 430K, Inhibitory effects of various ALK5 inhibitors on TGF−beta1-induced EMT in MCF10A cells. MCF10A cells were treated with TGF−beta1 (2 ng/ml) for 96 h in serum-reduced media (1% heat inactivated-horse serum (HI-HS)) in the presence or absence of the indicated ALK5 inhibitors. Medium was replaced every other day. Cell morphology was observed by phase-contrast microscopy (total magnification: x 100, scale bar: 100 microm).
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- 2023
4. Data from EW-7197, a Novel ALK-5 Kinase Inhibitor, Potently Inhibits Breast to Lung Metastasis
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Yhun Yhong Sheen, Jeong-Seok Nam, Dae-Kee Kim, Seung Won Kim, Min-Jin Kim, Sang-A. Park, Seon Joo Lee, Sol-Ji Kim, So-Yeon Park, and Ji Yeon Son
- Abstract
Advanced tumors produce an excessive amount of transforming growth factor β (TGFβ), which promotes tumor progression at late stages of malignancy. The purpose of this study was to develop anti-TGFβ therapeutics for cancer. We synthesized a novel small-molecule TGFβ receptor I kinase (activin receptor–like kinase 5) inhibitor termed N-[[4-([1,2,4]triazolo[1,5-a]pyridin-6-yl)-5-(6-methylpyridin-2-yl)-1H-imidazol-2-yl]methyl]-2-fluoroaniline (EW-7197), and we investigated its potential antimetastatic efficacy in mouse mammary tumor virus (MMTV)/c-Neu mice and 4T1 orthotopic–grafted mice. EW-7197 inhibited Smad/TGFβ signaling, cell migration, invasion, and lung metastasis in MMTV/c-Neu mice and 4T1 orthotopic–grafted mice. EW-7197 also inhibited the epithelial-to-mesenchymal transition (EMT) in both TGFβ-treated breast cancer cells and 4T1 orthotopic–grafted mice. Furthermore, EW-7197 enhanced cytotoxic T lymphocyte activity in 4T1 orthotopic–grafted mice and increased the survival time of 4T1-Luc and 4T1 breast tumor–bearing mice. In summary, EW-7197 showed potent in vivo antimetastatic activity, indicating its potential for use as an anticancer therapy. Mol Cancer Ther; 13(7); 1704–16. ©2014 AACR.
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- 2023
5. Supplementary Figure 6 from EW-7197, a Novel ALK-5 Kinase Inhibitor, Potently Inhibits Breast to Lung Metastasis
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Yhun Yhong Sheen, Jeong-Seok Nam, Dae-Kee Kim, Seung Won Kim, Min-Jin Kim, Sang-A. Park, Seon Joo Lee, Sol-Ji Kim, So-Yeon Park, and Ji Yeon Son
- Abstract
PDF - 80K, (A, B) Breast cancer model #6 (described in the Material and Methods). (A) Inhibition of lung metastasis by EW-7197 was evaluated by analysis of luciferase activity in lungs of 4T1-luc orthotropic xenograft mice. Statistical significance was defined using one-way ANOVA with Dunnett's multiple comparison test. * indicates significance at p
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- 2023
6. Supplementary Materials and Methods, Supplementary Figure Legends, and Supplementary Tables 1 through 3 from EW-7197, a Novel ALK-5 Kinase Inhibitor, Potently Inhibits Breast to Lung Metastasis
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Yhun Yhong Sheen, Jeong-Seok Nam, Dae-Kee Kim, Seung Won Kim, Min-Jin Kim, Sang-A. Park, Seon Joo Lee, Sol-Ji Kim, So-Yeon Park, and Ji Yeon Son
- Abstract
PDF - 191K, Supplementary materials and methods and supplementary figure legends. Supplementary Table 1. Antibodies for Western blot analysis. Supplementary Table 2. Primers for qRT-PCR or RT-PCR. Supplementary Table 3. Radiometric protein kinase assay.
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- 2023
7. Supplementary Figure 4 from EW-7197, a Novel ALK-5 Kinase Inhibitor, Potently Inhibits Breast to Lung Metastasis
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Yhun Yhong Sheen, Jeong-Seok Nam, Dae-Kee Kim, Seung Won Kim, Min-Jin Kim, Sang-A. Park, Seon Joo Lee, Sol-Ji Kim, So-Yeon Park, and Ji Yeon Son
- Abstract
PDF - 150K, Inhibitory effects of various ALK5 inhibitors on TGFbeta1-induced EMT in MCF10A cells. MCF10A cells were treated with TGFbeta1 (2 ng/ml) for 96 h in serum-reduced media (1% HI-HS) in the presence or absence of the indicated ALK5 inhibitors. Medium was replaced every other day. The protein levels of E-CADHERIN and N-CADHERIN were analyzed by western blotting as described in the Material and Methods. beta-ACTIN was used as endogenous control.
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- 2023
8. Supplementary Figure 1 from EW-7197, a Novel ALK-5 Kinase Inhibitor, Potently Inhibits Breast to Lung Metastasis
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Yhun Yhong Sheen, Jeong-Seok Nam, Dae-Kee Kim, Seung Won Kim, Min-Jin Kim, Sang-A. Park, Seon Joo Lee, Sol-Ji Kim, So-Yeon Park, and Ji Yeon Son
- Abstract
PDF - 120K, (A) Blockade of Smad2 phosphorylation by various ALK5 inhibitors. 4T1 cells starved in 0.2% HI-FBS medium for 24 h, and were treated with the indicated chemicals for 2 h with or without TGF-beta1 (2 ng/ml) in 0.2% HI-FBS medium. (B, C) Blockade of Smad2 phosphorylation by EW-7197. NMUMG (B) and MDA-MB-231 (C) cells were incubated with 0.2% HI-FBS medium for 24 h, and treated with TGF-beta1 (2 ng/ml) in the presence or absence of ALK5 inhibitors in 0.2% HI-FBS medium for 2 h. Lysates from the cells were analyzed by Western blotting. EW, SB, LY, IN indicate EW-7197, SB-505124, LY-2157299, and IN-1130, respectively.
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- 2023
9. Supplementary Figure 5 from EW-7197, a Novel ALK-5 Kinase Inhibitor, Potently Inhibits Breast to Lung Metastasis
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Yhun Yhong Sheen, Jeong-Seok Nam, Dae-Kee Kim, Seung Won Kim, Min-Jin Kim, Sang-A. Park, Seon Joo Lee, Sol-Ji Kim, So-Yeon Park, and Ji Yeon Son
- Abstract
PDF - 356K, (A~C) Breast cancer model #1, described in the Material and Methods. (A) Effect of EW-7197 on lung metastasis of breast cancer in MMTV-c/Neu mice. Representative images of H&E stained lungs (total magnification: x 12.5 or 100, scale bar: 800 μm or 100 μm). Total tumor volume (B) and body weight (C) in MMTV/c-Neu mice. Data represent the mean (plus or minus) SE (Veh: n=7, EW: n=10). (D and E) Body weight in breast cancer model #2 (D) and in breast cancer model #3 (E) (described in the Material and Methods). Data represent the mean (plus or minus) SE (n=10/group in Model #2 or n=6~8/group in Model #3). Veh, LY and EW indicate artificial gastric fluid, LY-2157299 and EW-7197, respectively. (F) In breast cancer model #3 (described in the Material and Methods), on day 28, two mice of each group were selected and treated with the indicated concentration of EW-7197. One mouse from each group was injected with TGF-beta1 (50 ng/mouse) through i.v. after 30 min and another was not injected. 90 min after TGF-beta1 injection, mice were sacrificed and lysates from primary mammary tumors were analyzed by western blotting as described in the Material and Methods.
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- 2023
10. Developing an Architecture of Robot Assembly Planning System based on Integrated CAD and BOM Databases
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Ji Yeon Son, Hyonyoung Han, and Namchul Do
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Assembly planning ,Computer science ,business.industry ,Robot ,CAD ,Architecture ,Software engineering ,business - Published
- 2021
11. Optimization of thermal annealing of zinc oxide films for enhancing performances of near-infrared organic photodetectors
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Ji Yeon Son, Hyeong Ju Eun, Gyeong Uk Seo, Ah Young Lee, Ju-Hyung Kim, and Jong H. Kim
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Process Chemistry and Technology ,General Chemical Engineering - Published
- 2023
12. Multi-agent system and reinforcement learning approach for distributed intelligence in a flexible smart manufacturing system
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Byung Do Chung, Yun Geon Kim, Seokgi Lee, Heechul Bae, and Ji-Yeon Son
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0209 industrial biotechnology ,Job shop scheduling ,Computer science ,Multi-agent system ,media_common.quotation_subject ,Distributed computing ,02 engineering and technology ,computer.software_genre ,Industrial and Manufacturing Engineering ,Intelligent agent ,Negotiation ,020901 industrial engineering & automation ,Hardware and Architecture ,Control and Systems Engineering ,0202 electrical engineering, electronic engineering, information engineering ,Production (economics) ,Reinforcement learning ,020201 artificial intelligence & image processing ,computer ,Productivity ,Software ,Smart manufacturing ,media_common - Abstract
Personalized production has emerged as a result of the increasing customer demand for more personalized products. Personalized production systems carry a greater amount of uncertainty and variability when compared with traditional manufacturing systems. In this paper, we present a smart manufacturing system using a multi-agent system and reinforcement learning, which is characterized by machines with intelligent agents to enable a system to have autonomy of decision making, sociability to interact with other systems, and intelligence to learn dynamically changing environments. In the proposed system, machines with intelligent agents evaluate the priorities of jobs and distribute them through negotiation. In addition, we propose methods for machines with intelligent agents to learn to make better decisions. The performance of the proposed system and the dispatching rule is demonstrated by comparing the results of the scheduling problem with early completion, productivity, and delay. The obtained results show that the manufacturing system with distributed artificial intelligence is competitive in a dynamic environment.
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- 2020
13. Exogenous Bio-Based 2,3-Butanediols Enhanced Abiotic Stress Tolerance of Tomato and Turfgrass under Drought or Chilling Stress
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Ae Ran Park, Jongmun Kim, Bora Kim, Areum Ha, Ji-Yeon Son, Chan Woo Song, Hyohak Song, and Jin-Cheol Kim
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Dehydration ,Solanum lycopersicum ,Gene Expression Regulation, Plant ,Stress, Physiological ,General Medicine ,Butylene Glycols ,Plants, Genetically Modified ,Applied Microbiology and Biotechnology ,Biotechnology ,Droughts ,Plant Proteins - Abstract
Among abiotic stresses in plants, drought and chilling stresses reduce the supply of moisture to plant tissues, inhibit photosynthesis, and severely reduce plant growth and yield. Thus, the application of water stress-tolerant agents can be a useful strategy to maintain plant growth under abiotic stresses. This study assessed the effect of exogenous bio-based 2,3-butanediol (BDO) application on drought and chilling response in tomato and turfgrass, and expression levels of several plant signaling pathway-related gene transcripts. Bio-based 2,3-BDOs were formulated to levo-2,3-BDO 0.9% soluble concentrate (levo 0.9% SL) and meso-2,3-BDO 9% SL (meso 9% SL). Under drought and chilling stress conditions, the application of levo 0.9% SL in creeping bentgrass and meso 9% SL in tomato plants significantly reduced the deleterious effects of abiotic stresses. Interestingly, pretreatment with levo-2,3-BDO in creeping bentgrass and meso-2,3-BDO in tomato plants enhanced JA and SA signaling pathway-related gene transcript expression levels in different ways. In addition, all tomato plants treated with acibenzolar
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- 2022
14. A New Histone Deacetylase Inhibitor, MHY4381, Induces Apoptosis via Generation of Reactive Oxygen Species in Human Prostate Cancer Cells
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In Su Kim, Su Hyun Lee, Prasanta Kumar Dey, Hyung Sik Kim, Chaeun Park, Jungho Yang, Hyung Ryong Moon, Sachan Richa, Jae Hyeon Park, Ji Yeon Son, and Mee-Young Ahn
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MHY4381 ,0301 basic medicine ,Programmed cell death ,medicine.drug_class ,Apoptosis ,urologic and male genital diseases ,Biochemistry ,03 medical and health sciences ,0302 clinical medicine ,HDAC inhibitor ,DU145 ,Drug Discovery ,LNCaP ,medicine ,Pharmacology ,Prostate cancer ,Cell growth ,Chemistry ,Histone deacetylase inhibitor ,Cell cycle ,030104 developmental biology ,030220 oncology & carcinogenesis ,Cancer cell ,Cancer research ,Molecular Medicine ,Original Article ,Reactive oxygen species - Abstract
Histone deacetylase (HDAC) inhibitors represent a novel class of anticancer agents, which can be used to inhibit cell proliferation and induce apoptosis in several types of cancer cells. In this study, we investigated the anticancer activity of MHY4381, a newly synthesized HDAC inhibitor, against human prostate cancer cell lines and compared its efficacy with that of suberoylanilide hydroxamic acid (SAHA), a well-known HDAC inhibitor. We assessed cell viability, apoptosis, cell cycle regulation, and other biological effects in the prostate cancer cells. We also evaluated a possible mechanism of MHY4381 on the apoptotic cell death pathway. The IC50 value of MHY4381 was lower in DU145 cells (IC50=0.31 μM) than in LNCaP (IC50=0.85 μM) and PC-3 cells (IC50=5.23 μM). In addition, the IC50 values of MHY4381 measured in this assay were significantly lower than those of SAHA against prostate cancer cell lines. MHY4381 increased the levels of acetylated histones H3 and H4 and reduced the expression of HDAC proteins in the prostate cancer cell lines. MHY4381 increased G2/M phase arrest in DU145 cells, and G1 arrest in LNCaP cells. It also activated reactive oxygen species (ROS) generation, which induced apoptosis in the DU145 and LNCaP cells by increasing the ratio of Bax/Bcl-2 and releasing cytochrome c into the cytoplasm. Our results indicated that MHY4381 preferentially results in antitumor effects in DU145 and LNCaP cells via mitochondria-mediated apoptosis and ROS-facilitated cell death pathway, and therefore can be used as a promising prostate cancer therapeutic.
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- 2020
15. The Effects of the Perception of Social Insurance System and Vocational Values on the Major Satisfaction of College Students Majoring in Beauty
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Ji-Yeon Son, Su-Yeon Eo, and Su-Gyung Kim
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Social insurance system ,media_common.quotation_subject ,Vocational education ,Perception ,Beauty ,Psychology ,Social psychology ,media_common - Published
- 2019
16. Modeling & Simulation-based Assessments of Reconfigurability and Productivity for Automotive Module Assembly Lines
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Jong Wan Lim, Sang Do Noh, Youngae Jeon, Sunhong Kim, Seohyeon Park, Sangho Lee, Yoo Ho Son, Hyunwoong Kong, Youngjun Jung, Jinho Yang, Myoung Jun Ko, and Ji Yeon Son
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Modeling and simulation ,Computer science ,business.industry ,Automotive industry ,Reconfigurability ,business ,Productivity ,Manufacturing engineering - Published
- 2019
17. Design and implementation of a digital twin application for a connected micro smart factory
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Ji Yeon Son, Sang Do Noh, Kyu Tae Park, Sung Ju Im, Young Wook Nam, Hyun Kim, and Hyeon Seung Lee
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Engineering ,business.industry ,Mechanical Engineering ,Smart factory ,Aerospace Engineering ,Manufacturing engineering ,Computer Science Applications ,Industrial technology ,Industrial Internet ,Production (economics) ,Electrical and Electronic Engineering ,business ,Smart manufacturing ,Distributed manufacturing - Abstract
Recently, manufacturing concepts, such as personalized production and distributed manufacturing, have attracted attention owing to the ongoing revolution in industrial technology. Connected micro s...
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- 2019
18. The FaaS system using additive manufacturing for personalized production
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Hyoung Seok Kang, Ji Yeon Son, Sang Do Noh, Ju Yeon Lee, Hyun Kim, and Jun-Hee Park
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0209 industrial biotechnology ,business.industry ,Computer science ,Mechanical Engineering ,Mass customization ,3D printing ,02 engineering and technology ,Virtualization ,computer.software_genre ,Industrial and Manufacturing Engineering ,Manufacturing engineering ,Personalization ,020901 industrial engineering & automation ,0202 electrical engineering, electronic engineering, information engineering ,Numerical control ,Factory (object-oriented programming) ,020201 artificial intelligence & image processing ,Manufacturing operations ,business ,computer ,Distributed manufacturing - Abstract
Purpose In this paper, a three-dimensional (3D) printer-based manufacturing line and supporting system, which supports personalized/customized manufacturing for individual businesses or start-up companies, was studied to evaluate the practicality of using additive manufacturing for personalization/mass customization. Design/methodology/approach First, factory-as-a-service (FaaS) system, which provides factory as a service to customers, was proposed and designed to manufacture various products within a distributed manufacturing environment. This system includes 3D printer-based material extrusion processes, vapor machine/computer numerical control machines as post-processes and assembly and inspection processes with an automated material handling robot in the factory. Second, a virtualization module for the FaaS factory was developed using a simulation model interfaced with a cloud-based order and production-planning system and an internet-of-things-based control and monitoring system. This is part of the system for manufacturing operations, which is capable of dynamic scheduling in a distributed manufacturing environment. In addition, simulation-based virtual production was conducted to verify and evaluate the FaaS factory for the target production scenario. Main information of the simulation also has been identified and included in the virtualization module. Finally, the established system was applied in a sample production scenario to evaluate its practicality and efficiency. Findings Additive manufacturing is a reliable, feasible and applicable technology, and it can be a core element in smart manufacturing and the realization of personalization/mass customization. Originality/value Various studies on additive manufacturing have been conducted with regard to replacing the existing manufacturing methods or integrating with them, but these studies mostly focused on materials or types of additive manufacturing, with few advanced or applied studies on the establishment of a new manufacturing environment for personalization/mass customization.
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- 2018
19. Corrigendum to 'Protective effect of EX-527 against high-fat diet-induced diabetic nephropathy in Zucker rats' [Toxicology and Applied Pharmacology 390 (2020) 114899]
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Prasanta Kumar Dey, Ji Yeon Son, Kwang Youl Lee, Hae Ri Kim, Amit Kundu, Kyeong Seok Kim, Sachan Richa, Sam Kacew, Byung-Hoon Lee, Seok-Yong Lee, Byung Mu Lee, and Hyung Sik Kim
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Pharmacology ,Diabetic nephropathy ,business.industry ,medicine ,High fat diet ,Zucker Rats ,Toxicology ,medicine.disease ,business - Published
- 2020
20. Aging-related Repositioned Drugs, Donepezil and Sildenafil Citrate, Increase Apoptosis of Anti-mitotic Drug-resistant KBV20C Cells Through Different Molecular Mechanisms
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Ji Yeong Kim, Hyung Sik Kim, Ji Yeon Son, Byung-Mu Lee, and Sungpil Yoon
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0301 basic medicine ,Drug ,Cancer Research ,ATP Binding Cassette Transporter, Subfamily B ,Cell Survival ,media_common.quotation_subject ,Down-Regulation ,Apoptosis ,Antimitotic Agents ,Pharmacology ,Sildenafil Citrate ,03 medical and health sciences ,0302 clinical medicine ,Piperidines ,Annexin ,Cell Line, Tumor ,Humans ,Medicine ,Cytotoxic T cell ,Donepezil ,Viability assay ,Furans ,Sensitization ,media_common ,business.industry ,Drug Repositioning ,Drug Synergism ,General Medicine ,Ketones ,Gene Expression Regulation, Neoplastic ,030104 developmental biology ,medicine.anatomical_structure ,Oncology ,Mechanism of action ,Drug Resistance, Neoplasm ,Vincristine ,030220 oncology & carcinogenesis ,Indans ,Cancer cell ,Carcinoma, Squamous Cell ,Mouth Neoplasms ,medicine.symptom ,business - Abstract
BACKGROUND/AIM The study focused on identifying the mechanisms or drugs that could sensitize P-glycoprotein (P-gp)-overexpressing resistant KBV20C cancer cells to halaven (HAL) or vincristine (VIC) treatment. MATERIALS AND METHODS Based on the relatively low dose or IC50 values for sensitizing anti-mitotic drug-resistant KBV20C cells, the aging-related drugs donepezil (DON) and sildenafil citrate (SID) were selected. Fluorescence-activated cell sorting (FACS), western blotting, and annexin V analyses were performed to investigate the mechanism of action of DON and SID in HAL-treated KBV20C cells. RESULTS DON or SID reduced cell viability, increased G2 arrest, and up-regulated the expression of the DNA damaging protein pH2AX when used as co-treatment with HAL. DON and SID induced both early and late apoptosis in KBV20C cells in response to HAL treatment, without increasing autophagy. VIC-DON and VIC-SID co-treatments increased sensitization of KBV20C cells, suggesting that DON and SID can be combined with other anti-mitotic drugs for sensitizing resistant cancer cells. When the sensitization efficacies of DON and SID were compared to that of the anti-psychotic repositioned drug fluphenazine (FLU), HAL-SID or HAL-FLU co-treatments were found to have better sensitization effects than HAL-DON suggesting that HAL-SID sensitization mechanism is different from that of HAL-DON. In addition, DON was found to have higher P-gp inhibitory activity than FLU or SID. CONCLUSION These results suggest that HAL-FLU or HAL-SID sensitization in KBV20C cells involves both cytotoxic and P-gp inhibitory effects, whereas HAL-DON sensitization may involve only P-gp inhibitory activity of DON.
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- 2018
21. Knockdown of Pyruvate Kinase M2 Inhibits Cell Proliferation, Metabolism, and Migration in Renal Cell Carcinoma
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Prasanta Kumar Dey, Kyungsil Yoon, Hyung Sik Kim, Amit Kundu, Byung Mu Lee, Kyeong Seok Kim, Yura Lee, Ki Taek Nam, Ji Yeon Son, and Sungpil Yoon
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Male ,Apoptosis ,migration ,lcsh:Chemistry ,Cell Movement ,Tumor Cells, Cultured ,Glycolysis ,lcsh:QH301-705.5 ,Spectroscopy ,Aged, 80 and over ,education.field_of_study ,Chemistry ,General Medicine ,Middle Aged ,Prognosis ,invasion ,Kidney Neoplasms ,Computer Science Applications ,Cell biology ,Gene Expression Regulation, Neoplastic ,Female ,Adult ,Thyroid Hormones ,autophagy ,Lactate dehydrogenase A ,PKM2 ,Catalysis ,Article ,Inorganic Chemistry ,Biomarkers, Tumor ,Humans ,Physical and Theoretical Chemistry ,education ,Molecular Biology ,Protein kinase B ,Carcinoma, Renal Cell ,PI3K/AKT/mTOR pathway ,Aged ,Cell Proliferation ,Cell growth ,Organic Chemistry ,Membrane Proteins ,lcsh:Biology (General) ,lcsh:QD1-999 ,Case-Control Studies ,Cancer cell ,pyruvate kinase M2 ,Carrier Proteins ,metabolism ,Pyruvate kinase ,Follow-Up Studies - Abstract
Emerging evidence indicates that the activity of pyruvate kinase M2 (PKM2) isoform is crucial for the survival of tumor cells. However, the molecular mechanism underlying the function of PKM2 in renal cancer is undetermined. Here, we reveal the overexpression of PKM2 in the proximal tubule of renal tumor tissues from 70 cases of patients with renal carcinoma. The functional role of PKM2 in human renal cancer cells following small-interfering RNA-mediated PKM2 knockdown, which retarded 786-O cell growth was examined. Targeting PKM2 affected the protein kinase B (AKT)/mechanistic target of the rapamycin 1 (mTOR) pathway, and downregulated the expression of glycolytic enzymes, including lactate dehydrogenase A and glucose transporter-1, and other downstream signaling key proteins. PKM2 knockdown changed glycolytic metabolism, mitochondrial function, adenosine triphosphate (ATP) level, and intracellular metabolite formation and significantly reduced 786-O cell migration and invasion. Acridine orange and monodansylcadaverine staining, immunocytochemistry, and immunoblotting analyses revealed the induction of autophagy in renal cancer cells following PKM2 knockdown. This is the first study to indicate PKM2/AKT/mTOR as an important regulatory axis mediating the changes in the metabolism of renal cancer cells.
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- 2019
22. A Study on Human-Robot Collaboration based Hybrid Assembly System for Flexible Manufacturing
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André Barthelmey, Hyun-Chul Kang, Eun-Seo Lee, Thorsten Reckelkamm, and Ji-Yeon Son
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Flexibility (engineering) ,0209 industrial biotechnology ,Computer science ,Testbed ,ComputerApplications_COMPUTERSINOTHERSYSTEMS ,02 engineering and technology ,010501 environmental sciences ,01 natural sciences ,Manufacturing engineering ,Human–robot interaction ,Variety (cybernetics) ,Product (business) ,020901 industrial engineering & automation ,Robot ,Production (economics) ,Productivity ,0105 earth and related environmental sciences - Abstract
Robot technology is one of the key technologies in the 4th industry revolution along with AI (Artificial Intelligent) and IoT (Internet of Things) technology, and robots that can collaborate with humans are drawing attention at industrial sites in particular. If only humans work on manufacturing sites without robots' help, a flexibility that can respond to a variety of variables can be increased, but productivity can be decreased. In the case of fully automated lines without human participation, productivity increases but lacks flexibility. In addition, it is very expensive to produce fully automated facilities that can be used in various product production processes. Therefore, a proper collaboration between humans and robots is a very important factor in ensuring productivity and flexibility. On the other hand, looking at future manufacturing trends, customer requirements for products are expected to be diversified and change rapidly in the future, which requires a flexible diversified small-quantity production environment, in which factories can produce a wide variety of products in a short period of time. In this paper, manufacturing facilities that can support various product production and precision assembly processes through collaboration between humans and robots, have been developed, and the developed facilities have been applied to two testbed lines respectively and functions are verified through actual product production.
- Published
- 2019
23. HSV Color Space Based Robot Grasping for Personalized Manufacturing Services
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Hyun Kim, Eun-Seo Lee, Min-Gi Kim, Hyonyoung Han, Ji-Yeon Son, Young-Kuk Kim, Hyun-Chul Kang, and Heechul Bae
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Functional verification ,Computer science ,Control system ,0502 economics and business ,05 social sciences ,Real-time computing ,Testbed ,Robot ,HSL and HSV ,050207 economics ,010501 environmental sciences ,01 natural sciences ,0105 earth and related environmental sciences - Abstract
Recently, with the development of artificial intelligence technology, robots have been utilized not only in manufacturing but also in many industrial fields. The needs of customers have been diversified and the trend of future manufacturing has been changing from the conventional mass production to the multiproduct small volume production system. In a smart factory environment for custom manufacturing, the robot must be manually programmed for each work instruction every time, especially when a production product is changed. Robot programming takes a lot of time and effort of people. In this paper, we propose a method to automatically measure the product information(color, size) automatically which are 3D printed out based on HSV (hue, saturation, value) Color Space and to control the auto grasping of robots. Also, we applied this control system to the FaaS(Factory As a Service) testbed for functional verification.
- Published
- 2019
24. A Study on the Effects of Information Acceptance Attitude of Beauty Services on User Satisfaction and Repurchase Intention through SNS
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Ji-Yeon Son
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0301 basic medicine ,03 medical and health sciences ,030109 nutrition & dietetics ,media_common.quotation_subject ,Beauty ,User satisfaction ,Advertising ,Psychology ,media_common - Published
- 2018
25. Curcumin ameliorates cadmium-induced nephrotoxicity in Sprague-Dawley rats
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Hyun Jung Lim, Byung Mu Lee, Jaewon Lee, Jong Seung Lim, Hyung Sik Kim, Kyeong Seok Kim, Ji Yeon Son, and Seung-Cheol Chang
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Male ,0301 basic medicine ,Curcumin ,Apoptosis ,010501 environmental sciences ,Pharmacology ,Kidney ,Toxicology ,01 natural sciences ,Nephrotoxicity ,Rats, Sprague-Dawley ,Excretion ,03 medical and health sciences ,chemistry.chemical_compound ,Oral administration ,Animals ,Humans ,Medicine ,Hepatitis A Virus Cellular Receptor 1 ,Blood urea nitrogen ,0105 earth and related environmental sciences ,Creatinine ,Tissue Inhibitor of Metalloproteinase-1 ,business.industry ,General Medicine ,Acute Kidney Injury ,Rats ,030104 developmental biology ,chemistry ,Blood chemistry ,Toxicity ,Osteopontin ,business ,Cadmium ,Food Science - Abstract
Chronic exposure to cadmium (Cd) causes remarkable damage to the kidneys, a target organ of accumulated Cd after oral administration. The aim of the present study was to investigate the protective effect of curcumin against Cd-induced nephrotoxicity. Sprague–Dawley male rats were divided into the following four treatment groups: control, curcumin (50 mg/kg, oral), CdCl2, (25 mg/kg, oral), and pre-treatment with curcumin (50 mg/kg) 1 h prior to the administration of CdCl2 (25 mg/kg, oral) for 7 days. At 24 h after the final treatment, the animals were killed, and the biomarkers associated with nephrotoxicity were measured. Our data indicated that blood urea nitrogen (BUN) and serum creatinine (sCr) levels were significantly reduced by curcumin pre-treatment in CdCl2-treated animals. Histopathological studies showed hydropic swelling and hypertrophy of the proximal tubular cells in the renal cortex after Cd treatment. Pretreatment with curcumin ameliorated the histological alterations induced by Cd. The urinary excretion of kidney injury molecule-1 (Kim-1), osteopontin (OPN), tissue inhibitor of metalloproteinases 1 (TIMP-1), neutrophil gelatinase-associated lipocalin (NGAL), and netrin-1 significantly reduced by curcumin treatment compared to that in the CdCl2-treated group. The administration of curcumin provided a significant protective effect against Cd-induced nephrotoxicity.
- Published
- 2018
26. Evaluation of subchronic exposure to triclosan on hepatorenal and reproductive toxicities in prepubertal male rats
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Yu Jin Park, Yong Hee Lee, Byung Mu Lee, Seung Jun Kwack, Hyung Sik Kim, Lee Ena, Ji Yeon Son, Mee-Young Ahn, Jong Seung Lim, and Ji Yeong Kim
- Subjects
Male ,0301 basic medicine ,medicine.medical_specialty ,Health, Toxicology and Mutagenesis ,010501 environmental sciences ,Kidney ,Toxicology ,01 natural sciences ,Rats, Sprague-Dawley ,Random Allocation ,03 medical and health sciences ,chemistry.chemical_compound ,Internal medicine ,Adrenal Glands ,Testis ,Animals ,Medicine ,Blood urea nitrogen ,0105 earth and related environmental sciences ,Creatinine ,Dose-Response Relationship, Drug ,business.industry ,Reproduction ,Toxicity Tests, Subchronic ,fungi ,Malondialdehyde ,Triclosan ,Rats ,Dose–response relationship ,030104 developmental biology ,medicine.anatomical_structure ,Endocrinology ,Liver ,chemistry ,Toxicity ,Anti-Infective Agents, Local ,business ,Spermatogenesis - Abstract
Triclosan (TCS), a common antimicrobial ingredient, is present in many consumer products, including soaps, shampoos, and toothpaste. Owing to its widespread use, potential adverse effects on animals and humans may arise from lifetime exposure, but data on chronic prepubertal exposure of TCS are still lacking. The aim of the present study was to investigate the influence of subchronic TCS exposure (0.25, 25, 250, or 750 mg/kg) on target organ toxicity in prepubertal male rats. After daily administration of TCS to rats by oral gavage for 60 d, a significant reduction in body weight and relative weights of liver, kidneys, testes, and adrenal glands was observed in the 750-mg/kg (high dose) group. Serum alanine aminotransferase and aspartate aminotransferase activities as well as levels of blood urea nitrogen, and creatinine were significantly increased at 750 mg/kg TCS. Further, TCS (750 mg/kg) elevated the protein expressions of hepatic CYP2B1, RXR/PPAR, and levels of malondialdehyde. High-dose TCS exposure induced histological changes as evidenced by reduction of Bowman's space, occlusion of the tubular lumen, and degeneration of tubular epithelial cells in the kidney. Tubular necrosis was confirmed as evidenced by a rise in expression of high mobility group box 1 renal protein. Daily sperm production was significantly diminished by high doses of TCS with marked inhibition of androgen receptor protein expression. Our results indicated that subchronic exposure to excessively high concentrations of 750 mg/kg TCS induced hepatorenal and reproductive toxicities in prepubertal male rats; however, the biological relevance of these findings is questionable as these drug levels are not encountered in the environment.
- Published
- 2018
27. HSV Color-Space-Based Automated Object Localization for Robot Grasping without Prior Knowledge
- Author
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Heechul Bae, Hyonyoung Han, Min-Gi Kim, Young-Kuk Kim, Hyun-Chul Kang, and Ji-Yeon Son
- Subjects
Technology ,Similarity (geometry) ,QH301-705.5 ,Computer science ,Machine vision ,QC1-999 ,ComputingMethodologies_IMAGEPROCESSINGANDCOMPUTERVISION ,HSL and HSV ,HSV color space ,General Materials Science ,Computer vision ,Biology (General) ,teaching-less robot ,QD1-999 ,Instrumentation ,Fluid Flow and Transfer Processes ,business.industry ,Physics ,Process Chemistry and Technology ,smart factory ,General Engineering ,Engineering (General). Civil engineering (General) ,Object (computer science) ,Automation ,Computer Science Applications ,Chemistry ,Robot ,Artificial intelligence ,Noise (video) ,TA1-2040 ,business ,Reset (computing) ,object localization - Abstract
We propose a simple and robust HSV color-space-based algorithm that can automatically extract object position information without human intervention or prior knowledge. In manufacturing sites with high variability, it is difficult to recognize products through robot machine vision, especially in terms of extracting object information accurately, owing to various environmental factors such as the noise around objects, shadows, light reflections, and illumination interferences. The proposed algorithm, which does not require users to reset the HSV color threshold value whenever a product is changed, uses ROI referencing method to solve this problem. The algorithm automatically identifies the object’s location by using the HSV color-space-based ROI random sampling, ROI similarity comparison, and ROI merging. The proposed system utilizes an IoT device with several modules for the detection, analysis, control, and management of object data. The experimental results show that the proposed algorithm is very useful for industrial automation applications under complex and highly variable manufacturing environments.
- Published
- 2021
28. Curcumin Ameliorates Benzo[a]pyrene-Induced DNA Damages in Stomach Tissues of Sprague-Dawley Rats
- Author
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Byung Mu Lee, Boomin Kim, Ji Yeon Son, Kyeong Seok Kim, Hae Ri Kim, Hyung Sik Kim, Na Yoon Kim, Jae Hyeon Park, Hye Gwang Jeong, Su Hyun Lee, and Yoon Gyoon Kim
- Subjects
0301 basic medicine ,Pharmacology ,Kidney ,lcsh:Chemistry ,Rats, Sprague-Dawley ,chemistry.chemical_compound ,DNA Adducts ,0302 clinical medicine ,Cytochrome P-450 Enzyme System ,polycyclic compounds ,lcsh:QH301-705.5 ,Spectroscopy ,biology ,Stomach ,General Medicine ,Organ Size ,Computer Science Applications ,medicine.anatomical_structure ,Benzo(a)pyrene ,Liver ,8-Hydroxy-2'-Deoxyguanosine ,030220 oncology & carcinogenesis ,embryonic structures ,Metabolome ,animal structures ,Curcumin ,CYP1B1 ,gastrointestinal cancer ,complex mixtures ,Catalysis ,Article ,BPDE-I-DNA adduct ,Inorganic Chemistry ,03 medical and health sciences ,medicine ,Animals ,Gastrointestinal cancer ,Physical and Theoretical Chemistry ,Molecular Biology ,Carcinogen ,organic chemicals ,Organic Chemistry ,Body Weight ,Cytochrome P450 ,medicine.disease ,030104 developmental biology ,chemistry ,lcsh:Biology (General) ,lcsh:QD1-999 ,biology.protein ,8-hydroxydeoxy guanosine ,DNA Damage - Abstract
Benzo[a]pyrene (BaP) is a well-known carcinogen formed during the cooking process. Although BaP exposure has been implicated as one of the risk factors for lung cancer in animals and humans, there are only limited data on BaP-induced gastrointestinal cancer. Therefore, this study investigated the protective effects of curcumin on BaP-induced DNA damage in rat stomach tissues. BaP (20 mg/kg/day) and curcumin (50, 100, or 200 mg/kg) were administered daily to Sprague-Dawley rats by oral gavage over 30 days. Curcumin was pre-administered before BaP exposure. All rats were euthanized, and liver, kidney, and stomach tissues were removed at 24 h after the last treatment. We observed that aspartate aminotransferase (AST), alanine aminotransferase (ALT), and glucose levels were significantly reduced in rats treated with high dose co-administration of curcumin (200 mg/kg) compared to BaP alone. The expression levels of cytochrome P450 (CYP) 1A1 and CYP1B1 were significantly increased in the liver of rats treated with BaP. However, co-administration of curcumin (200 mg/kg) with BaP markedly reduced CYP1A1 expression in a dose-dependent manner. Furthermore, plasma levels of BaP-diolepoxide (BPDE) and BaP metabolites were significantly reduced by co-administration of curcumin (200 mg/kg). Additionally, co-administration of curcumin (200 mg/kg) with BaP significantly reduced the formation of BPDE-I-DNA and 8-hydroxydeoxy guanosine (8-OHdG) adducts in the liver, kidney, and stomach tissues. The inhibition of these adduct formations were more prominent in the stomach tissues than in the liver. Overall, our observations suggest that curcumin might inhibit BaP-induced gastrointestinal tumorigenesis and shows promise as a chemopreventive agent.
- Published
- 2019
29. Protective effect of EX-527 against high-fat diet-induced diabetic nephropathy in Zucker rats
- Author
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Sam Kacew, Byung Mu Lee, Amit Kundu, Hyung Sik Kim, Prasanta Kumar Dey, Sachan Richa, Hae Ri Kim, Byung-Hoon Lee, Seok-Yong Lee, Kwang Youl Lee, Kyeong Seok Kim, and Ji Yeon Son
- Subjects
0301 basic medicine ,Blood Glucose ,Glycation End Products, Advanced ,Male ,medicine.medical_specialty ,Carbazoles ,Inflammation ,Toxicology ,medicine.disease_cause ,Diet, High-Fat ,Kidney ,Proinflammatory cytokine ,Diabetic nephropathy ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Internal medicine ,medicine ,Animals ,Diabetic Nephropathies ,Blood urea nitrogen ,Pancreas ,Pharmacology ,Creatinine ,business.industry ,Glomerulosclerosis ,Interleukin ,Organ Size ,medicine.disease ,Rats ,Rats, Zucker ,Oxidative Stress ,030104 developmental biology ,Endocrinology ,chemistry ,Gene Expression Regulation ,030220 oncology & carcinogenesis ,Cytokines ,medicine.symptom ,business ,Oxidative stress ,Biomarkers - Abstract
High-fat diet (HFD)-induced obesity is implicated in diabetic nephropathy (DN). EX-527, a selective Sirtuin 1 (SIRT1) inhibitor, has multiple biological functions; however, its protective effect against DN is yet to be properly understood. This study was aimed to explore the protective effect of EX-527 against DN in HFD-induced diabetic Zucker (ZDF) rats. After 21 weeks of continually feeding HFD to the rats, the apparent characteristics of progressive DN were observed, which included an increase in kidney weight (~160%), hyperglycemia, oxidative stress, and inflammatory cytokines, and subsequent renal cell damage. However, the administration of EX-527 for 10 weeks significantly reduced the blood glucose concentration and kidney weight (~59%). Furthermore, EX-527 significantly reduced the serum concentration of transforming growth factor-β1 (49%), interleukin (IL)-1β (52%), and IL-6 in the HFD-fed rats. Overall, the antioxidant activities significantly increased, and oxidative damage to lipids or DNA was suppressed. Particularly, EX-527 significantly reduced blood urea nitrogen (81%), serum creatinine (71%), microalbumin (43%), and urinary excretion of protein-based biomarkers. Histopathological examination revealed expansion of the extracellular mesangial matrix and suppression of glomerulosclerosis following EX-527 administration. EX-527 downregulated the expression of α-SMA (~64%), TGF-β (25%), vimentin, α-tubulin, fibronectin, and collagen-1 in the kidneys of the HFD-fed rats. Additionally, EX-527 substantially reduced claudin-1 and SIRT1 expression, but increased the expression of SIRT3 in the kidneys of the HFD-fed rats. EX-527 also inhibited the growth factor receptors, including EGFR, PDGFR-β, and STAT3, which are responsible for the anti-fibrotic effect of SIRT-1. Therefore, the administration of EX-527 protects against HFD-induced DN.
- Published
- 2019
30. Selenium-binding protein 1: a sensitive urinary biomarker to detect heavy metal-induced nephrotoxicity
- Author
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Eui Kyung Lee, Byung Mu Lee, Aree Moon, Eun Young Park, Ok-Nam Bae, Hyung Sik Kim, Ji Yeon Son, Nam Deuk Kim, Seung Jun Kwack, Young-Jun Shin, and Sam Kacew
- Subjects
Male ,Proteomics ,0301 basic medicine ,Pathology ,medicine.medical_specialty ,Kidney Cortex ,Health, Toxicology and Mutagenesis ,Urinary system ,Selenium-Binding Proteins ,Toxicology ,Sensitivity and Specificity ,Blood Urea Nitrogen ,Nephrotoxicity ,Rats, Sprague-Dawley ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Cadmium Chloride ,Metals, Heavy ,Internal medicine ,medicine ,Animals ,Electrophoresis, Gel, Two-Dimensional ,Selenium binding ,Blood urea nitrogen ,Creatinine ,Kidney ,Dose-Response Relationship, Drug ,Clusterin ,biology ,Proteins ,General Medicine ,Rats ,030104 developmental biology ,Endocrinology ,medicine.anatomical_structure ,chemistry ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,030220 oncology & carcinogenesis ,Mercuric Chloride ,biology.protein ,Biomarker (medicine) ,Kidney Diseases ,Cisplatin ,Biomarkers - Abstract
Identifying novel biomarkers to detect nephrotoxicity is clinically important. Here, we attempted to identify new biomarkers for mercury-induced nephrotoxicity and compared their sensitivity to that of traditional biomarkers in animal models. Comparative proteomics analysis was performed in kidney tissues of Sprague–Dawley rats after oral treatment with HgCl2 (0.1, 1, or 5 mg/kg/day) for 21 days. Kidney cortex tissues were analyzed by two-dimensional gel electrophoresis/matrix-assisted laser desorption/ionization, and differentially expressed proteins were identified. The corresponding spots were quantitated by RT-PCR. Selenium-binding protein 1 (SBP1) was found to be the most markedly upregulated protein in the kidney cortex of rats after HgCl2 administration. However, blood urea nitrogen, serum creatinine, and glucose levels increased significantly only in the 1 or 5 mg/kg HgCl2-treated groups. A number of urinary excretion proteins, including kidney injury molecule-1, clusterin, monocyte chemoattractant protein-1, and β-microglobulin, increased dose-dependently. Histopathological examination revealed severe proximal tubular damage in high-dose (5 mg/kg) HgCl2-exposed groups. In addition, urinary excretion of SBP1 significantly increased in a dose-dependent manner. To confirm the critical role of SBP1 as a biomarker for nephrotoxicity, normal kidney proximal tubular cells were treated with HgCl2, CdCl2, or cisplatin for 24 h. SBP1 levels significantly increased in conditioned media exposed to nephrotoxicants, but decreased in cell lysates. Our investigations suggest that SBP1 may play a critical role in the pathological processes underlying chemical-induced nephrotoxicity. Thus, urinary excretion of SBP1 might be a sensitive and specific biomarker to detect early stages of kidney injury.
- Published
- 2016
31. Smart manufacturing: Past research, present findings, and future directions
- Author
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SangSu Choi, Ji Yeon Son, Ju Yeon Lee, Bo Hyun Kim, Hyun Kim, Hyoung Seok Kang, Jun Hee Park, and Sang Do Noh
- Subjects
0209 industrial biotechnology ,Engineering ,Industry 4.0 ,Renewable Energy, Sustainability and the Environment ,business.industry ,Mechanical Engineering ,Integrated Computer-Aided Manufacturing ,05 social sciences ,Cyber-physical system ,02 engineering and technology ,Industrial and Manufacturing Engineering ,Manufacturing engineering ,020901 industrial engineering & automation ,Computer-integrated manufacturing ,Information and Communications Technology ,Management of Technology and Innovation ,Manufacturing ,0502 economics and business ,Advanced manufacturing ,General Materials Science ,Technology roadmap ,business ,050203 business & management - Abstract
Today, the manufacturing industry is aiming to improve competitiveness through the convergence with cutting-edge ICT technologies in order to secure a new growth engine. Smart Manufacturing, which is the fourth revolution in the manufacturing industry and is also considered as a new paradigm, is the collection of cutting-edge technologies that support effective and accurate engineering decision-making in real time through the introduction of various ICT technologies and the convergence with the existing manufacturing technologies. This paper surveyed and analyzed various articles related to Smart Manufacturing, identified the past and present levels, and predicted the future. For these purposes, 1) the major key technologies related to Smart Manufacturing were identified through the analysis of the policies and technology roadmaps of Germany, the U.S., and Korea that have government-driven leading movements for Smart Manufacturing, 2) the related articles on the overall Smart Manufacturing concept, the key system structure, or each key technology were investigated, and, finally, 3) the Smart Manufacturing-related trends were identified and the future was predicted by conducting various analyses on the application areas and technology development levels that have been addressed in each article.
- Published
- 2016
32. Dendropanax morbifera Protects against Renal Fibrosis in Streptozotocin-Induced Diabetic Rats
- Author
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Byung Mu Lee, Su Hyun Lee, Shugeng Cao, Ji Yeon Son, Richa Sachan, Hyung Sik Kim, Jung-Hwan Kim, Prasanta Kumar Dey, Hae Ri Kim, Amit Kundu, Kyeong Seok Kim, Jae Hyeon Park, Da Eun Lee, and Jong Hwan Kwak
- Subjects
0301 basic medicine ,dendropanax morbifera ,Physiology ,Clinical Biochemistry ,Dendropanax morbifera ,Pharmacology ,streptozotocin ,Biochemistry ,Article ,Diabetic nephropathy ,03 medical and health sciences ,0302 clinical medicine ,Fibrosis ,Diabetes mellitus ,medicine ,Renal fibrosis ,oxidative stress ,Selenium binding ,Molecular Biology ,Blood urea nitrogen ,Kidney ,business.industry ,diabetic nephropathy ,lcsh:RM1-950 ,Cell Biology ,medicine.disease ,Streptozotocin ,lcsh:Therapeutics. Pharmacology ,030104 developmental biology ,medicine.anatomical_structure ,inflammation ,030220 oncology & carcinogenesis ,business ,medicine.drug - Abstract
The aquatic extract of Dendropanax morbifera (DP) is typically consumed as a beverage in Korea and China and is also used in various traditional medicines. However, the functional role of DP on diabetes-induced renal fibrosis is unclear. Here, the protective effects of DP extract against diabetes-induced renal fibrosis were evaluated. Streptozotocin (STZ, 60 mg/kg) was injected intraperitoneally in rats to induce diabetes. After 5 days, DP extract (25 mg/kg/day) and metformin (50 mg/kg/day) were administered orally to diabetic rats for 28 days. DP administration protected both body and organ weight loss in STZ-treated diabetic rats. Significant improvements in serum blood urea nitrogen (BUN), creatinine, and oxidative stress parameters were observed in diabetic rats by DP administration. DP extract markedly protected diabetic-induced histopathological damages in the kidney and pancreas. A significant reduction was observed in microalbumin, kidney injury molecule-1 (KIM-1), selenium binding protein-1 (SBP1), and pyruvate kinase muscle isozyme M2 (PKM2) levels in the urinary excretion of diabetic rats after the administration of DP extract. The expression of pro-inflammatory cytokines and fibrosis marker levels were significantly reduced in the kidney of diabetic rats. Our results strongly indicate that DP extract exhibits protective activity against diabetes-induced renal fibrosis through ameliorating oxidative stress and inflammation. Therefore, we suggest that DP extract can be used as a preventive agent on the progression of diabetic nephropathy and renal fibrosis.
- Published
- 2020
33. Novel SIRT1 inhibitor 15-deoxy-Δ12,14-prostaglandin J2 and its derivatives exhibit anticancer activity through apoptotic or autophagic cell death pathways in SKOV3 cells
- Author
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Hyung Sik Kim, In Hwan Tae, Ji Yeon Son, Seok-Yong Lee, Mi-hyun Kim, Prasanta Kumar Dey, Saloni Saloni, Eun Young Park, and Jee H. Jung
- Subjects
Models, Molecular ,0301 basic medicine ,autophagy ,Cancer Research ,Programmed cell death ,Cell cycle checkpoint ,Cell Survival ,Cell ,Antineoplastic Agents ,Biology ,sirtuin 1 ,03 medical and health sciences ,0302 clinical medicine ,Cell Line, Tumor ,medicine ,Humans ,MTT assay ,Viability assay ,Ovarian Neoplasms ,Dose-Response Relationship, Drug ,Prostaglandin D2 ,Cell Cycle ,apoptosis ,Articles ,Cell cycle ,Gene Expression Regulation, Neoplastic ,Molecular Docking Simulation ,15-deoxy-Δ12,14-prostaglandin J2 ,J11-Cl ,ovarian cancer ,030104 developmental biology ,medicine.anatomical_structure ,Oncology ,Apoptosis ,Cell culture ,030220 oncology & carcinogenesis ,Cancer research ,Beclin-1 ,Female ,Microtubule-Associated Proteins ,Signal Transduction - Abstract
Clinically relevant sirtuin (SIRT) inhibitors may possess antitumor activities. A previous study indicated that 15-deoxy-Δ12,14-prostaglandin J2 (15d-PGJ2) exhibited potent anticancer activity by SIRT1 inhibition. Therefore, the aim of the present study was to investigate whether its derivatives (J11-C1 and J19) exhibited anticancer activity against ovarian cancer SKOV3 cells. Cell viability was determined using an MTT assay. Cell cycle arrest, apoptosis and autophagy were determined using flow cytometry or western blot analysis. J11-Cl and J19 were less cytotoxic to SKOV3 cells compared with 15d-PGJ2. Molecular docking studies supported the interactions of 15d-PGJ2, J11-Cl and J19 with various amino acids in SIRT1 proteins. Similar to 15d-PGJ2, J11-C1 and J19 inhibited SIRT1 enzymatic activity and decreased SIRT1 expression levels in a concentration-dependent manner. J11-C1 induced apoptotic cell death more effectively compared with J19, which was associated with markedly decreased expression of the anti-apoptotic molecule B-cell lymphoma 2 (Bcl-2). Furthermore, the levels of light chain 3-II (LC3-II) and beclin-1 were clearly induced in SKOV3 cells treated with J11-Cl. Thus, 15d-PGJ2 and its derivatives exhibited anticancer activity possibly by inducing apoptotic or autophagic cell death pathways. Collectively, the results of the present study suggest that 15d-PGJ2 and its derivatives exerted antitumor activity by selectively modulating the expression of genes associated with cell cycle arrest, apoptosis and autophagy. Notably, J11-C1 is a novel candidate SIRT1 inhibitor with anticancer activity.
- Published
- 2018
34. Plumbagin from a tropical pitcher plant (Nepenthes alata Blanco) induces apoptotic cell death via a p53-dependent pathway in MCF-7 human breast cancer cells
- Author
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In Su Kim, Wahn Soo Choi, Song-Yi Ha, Umasankar De, Jong Hwan Kwak, Yu Jin Park, Byung Mu Lee, Hyung Sik Kim, Ki-Tae Ha, Yukyoung Jeon, Sungpil Yoon, and Ji Yeon Son
- Subjects
Cell cycle checkpoint ,Mice, Nude ,Apoptosis ,Breast Neoplasms ,Toxicology ,03 medical and health sciences ,chemistry.chemical_compound ,0404 agricultural biotechnology ,Cell Line, Tumor ,Animals ,Humans ,Cytotoxicity ,030304 developmental biology ,Cell Proliferation ,0303 health sciences ,Mice, Inbred BALB C ,Cell Cycle ,04 agricultural and veterinary sciences ,General Medicine ,Plumbagin ,Cell cycle ,040401 food science ,Antineoplastic Agents, Phytogenic ,Caryophyllales ,chemistry ,MCF-7 ,Cancer cell ,Cancer research ,MCF-7 Cells ,Female ,Tumor Suppressor Protein p53 ,Reactive Oxygen Species ,Intracellular ,Food Science ,Naphthoquinones - Abstract
Plumbagin (5-hydroxy-2-methyl-1,4-naphthaquinone) has displayed antitumor activity in vitro and in animal models; however, the underlying molecular mechanisms have not been fully explored. The aim of this study was to investigate the anticancer effects of plumbagin isolated from Nepenthes alata against MCF-7 breast cancer cells. We examined the cytotoxicity, cell cycle regulation, apoptotic cell death, and generation of intracellular reactive oxygen species (ROS) in MCF-7 cells. Plumbagin exhibited potent cytotoxicity in MCF-7 cells (wild-type p53) compared to that in SK-OV-3 (null-type) human epithelial ovarian cancer cells. Specifically, plumbagin upregulated the expression of p21CIP1/WAF1 in MCF-7 cells, causing cell cycle arrest in the G2/M phase through inhibition of cyclin B1 levels. Plumbagin also significantly increased the ratio of Bax/Bcl-2 and release of cytochrome c, resulting in apoptotic cell death in MCF-7 cells. Furthermore, plumbagin dramatically increased the intracellular ROS level, whereas pretreatment with the ROS scavenger N-acetyl cysteine protected against plumbagin-induced cytotoxicity, suggesting that ROS formation plays a pivotal role in antitumor activity in MCF-7 cells. In mice bearing MCF-7 cell xenografts, plumbagin significantly reduced tumor growth and weight without apparent side effects. We therefore concluded that plumbagin exerts anticancer activity against MCF-7 cells through the generation of intracellular ROS, resulting in the induction of apoptosis via a p53-dependent pathway. This study thus identifies a new anticancer mechanism of plumbagin against p53-dependent breast cancer cells and suggests a novel strategy for overcoming of breast cancer therapy.
- Published
- 2018
35. Hepatic damage exacerbates cisplatin-induced acute kidney injury in Sprague-Dawley rats
- Author
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Hyun Jung Lim, Kwang Youl Lee, Kyeong Seok Kim, Jaewon Lee, Hyung Sik Kim, Young-Mi Kim, Ji Yeon Son, Mee-Young Ahn, Byung Mu Lee, Seung Jun Kwack, and Ji-Su Kim
- Subjects
inorganic chemicals ,0301 basic medicine ,Male ,Health, Toxicology and Mutagenesis ,Antineoplastic Agents ,Pharmacology ,Thioacetamide ,Toxicology ,Nephrotoxicity ,Rats, Sprague-Dawley ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Medicine ,Animals ,neoplasms ,Blood urea nitrogen ,Cisplatin ,Liver injury ,Kidney ,Creatinine ,business.industry ,Acute kidney injury ,Acute Kidney Injury ,medicine.disease ,digestive system diseases ,female genital diseases and pregnancy complications ,Rats ,030104 developmental biology ,medicine.anatomical_structure ,chemistry ,Liver ,030220 oncology & carcinogenesis ,Carcinogens ,business ,Biomarkers ,medicine.drug - Abstract
The objective of this study was to elucidate the effect of hepatic damage on cisplatin (CDDP)-induced acute kidney injury (AKI). Thioacetamide (TAA, 150 mg/kg), a hepatotoxicant, was intraperitoneally (i.p.) injected to male Sprague-Dawley rats for 3 d prior to CDDP (5 mg/kg, i.p.) injection. All animals were sacrificed 5 d after CDDP treatment, and urine or blood was obtained to measure various parameters. No significant changes in serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activity were observed after CDDP treatment. However, pretreatment with TAA significantly elevated ALT and AST activity. Serum blood urea nitrogen and creatinine levels significantly increased in CDDP-treated group compared to control. In addition, urinary excretion of novel protein-based biomarkers such as neutrophil gelatinase-associated lipocalin, vascular endothelial growth factor, kidney injury molecule-1, and tissue inhibitor of metalloproteinase-1 rose markedly in the CDDP-treated group. In particular, pretreatment with TAA markedly elevated CDDP-induced urinary excretion of protein-based nephrotoxic biomarkers compared with CDDP alone. Hematoxylin and eosin staining demonstrated that pretreatment with TAA following CDDP injection led to more severe tubular damage and apoptosis in rats compared with CDDP alone. Antioxidant status was significantly reduced in kidneys following pretreatment with TAA prior to CDDP. These findings indicate that liver injury enhanced the vulnerability of kidney to CDDP-induced AKI and this phenomenon may be associated with severe apoptotic damage.
- Published
- 2018
36. Novel therapeutic roles of MC-4 in combination with everolimus against advanced renal cell carcinoma by dual targeting of Akt/pyruvate kinase muscle isozyme M2 and mechanistic target of rapamycin complex 1 pathways
- Author
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Ji Yeon Son, Hye Won Lee, Young Ju Park, Jong Hwan Kwak, Sungpil Yoon, Im Joo Rhyu, Han Yong Choi, Joung Eun Lim, Kyu Huck Chung, Se Jeong Lee, In Hwan Tae, Umasankar De, Yukyoung Jeon, Hyung Sik Kim, Byung Mu Lee, and Yu Jin Park
- Subjects
0301 basic medicine ,Cancer Research ,Thyroid Hormones ,autophagy ,Cell Survival ,mTORC1 ,PKM2 ,Artemisia annua ,Mechanistic Target of Rapamycin Complex 1 ,urologic and male genital diseases ,metastatic renal cell carcinoma ,03 medical and health sciences ,Mice ,0302 clinical medicine ,Cell Line, Tumor ,Antineoplastic Combined Chemotherapy Protocols ,medicine ,Animals ,Humans ,Radiology, Nuclear Medicine and imaging ,pyruvate kinase muscle isozyme M2 ,Everolimus ,Protein kinase B ,Carcinoma, Renal Cell ,PI3K/AKT/mTOR pathway ,Cell Proliferation ,Original Research ,Cancer Biology ,Artemisia annua L ,Chemistry ,Cell growth ,Plant Extracts ,Autophagy ,Membrane Proteins ,Plant Components, Aerial ,Xenograft Model Antitumor Assays ,Kidney Neoplasms ,Gene Expression Regulation, Neoplastic ,030104 developmental biology ,Oncology ,030220 oncology & carcinogenesis ,Cancer cell ,Cancer research ,Female ,Carrier Proteins ,Proto-Oncogene Proteins c-akt ,medicine.drug ,Signal Transduction - Abstract
Current clinical trials of new anticancer therapies against metastatic renal cell carcinoma (RCC), including molecular‐targeted therapies, have not shown promise. The purpose of this study was to preclinically assess the antitumor effects of MC‐4, a partially purified material of Artemisia annua L., as a monotherapy or in combination with the known mechanistic target of rapamycin complex 1 (mTORC1) inhibitor, everolimus, against Caki‐1 (Von Hippel‐Lindau (VHL)+/+) and 786‐O (VHL−/−) human RCC cells. MC‐4 monotherapy significantly increased tumor growth inhibition and autophagic cell death in RCC cells in vitro and in vivo. Everolimus led to compensatory Akt activation by inhibiting only mTORC1 signaling pathway. In contrast to everolimus, MC‐4 enhanced phosphatase and tensin homolog expression and reduced its downstream effector, Akt/pyruvate kinase muscle isozyme M2 (PKM2), leading to decreased expression of glucose transporter 1, which is associated with cancer cell metabolism. The synergistic antitumor and anti‐metastatic effects induced by co‐administration of MC‐4 and everolimus involve cell growth inhibition and autophagic cell death via dual targeting of phosphatidylinositol 3‐kinase (PI3K)/Akt/PKM2 and mTORC1. These findings suggest that MC‐4 is a novel Akt/PKM2 inhibitor that can overcome the limitation of existing mTOR inhibitors and can be considered a novel strategy to treat patients with rapidly progressing advanced RCC.
- Published
- 2018
37. Identification of a sensitive urinary biomarker, selenium-binding protein 1, for early detection of acute kidney injury
- Author
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Kyeong Seok Kim, Seung Jun Kwack, Hosup Song, JiHoon Kwon, JiHye An, Hun Yong Yang, Ye Rim Kang, Jaewon Lee, Ok-Nam Bae, Sungpil Yoon, Hyung Sik Kim, Byung Mu Lee, Young-Mi Kim, Ji Yeon Son, and Mee-Young Ahn
- Subjects
0301 basic medicine ,Adult ,Male ,Proteomics ,medicine.medical_specialty ,Pathology ,Health, Toxicology and Mutagenesis ,Urinary system ,Urology ,Urine ,Selenium-Binding Proteins ,Toxicology ,Nephrotoxicity ,Rats, Sprague-Dawley ,03 medical and health sciences ,chemistry.chemical_compound ,Predictive Value of Tests ,medicine ,Animals ,Humans ,Selenium binding ,Blood urea nitrogen ,Aged ,Aged, 80 and over ,Creatinine ,business.industry ,Area under the curve ,Acute kidney injury ,Reproducibility of Results ,Acute Kidney Injury ,Middle Aged ,medicine.disease ,Rats ,030104 developmental biology ,Early Diagnosis ,chemistry ,Models, Animal ,Female ,business ,Biomarkers - Abstract
Acute kidney injury (AKI) is associated with increased mortality rate in patients but clinically available biomarkers for disease detection are currently not available. Recently, a new biomarker, selenium-binding protein 1 (SBP1), was identified for detection of nephrotoxicity using proteomic analysis. The aim of this study was to assess the sensitivity of urinary SBP1 levels as an early detection of AKI using animal models such as cisplatin or ischemia/reperfusion (I/R). Sprague-Dawley rats were injected with cisplatin (6 mg/kg, once i.p.) and sacrificed at 1, 3, or 5 days after treatment. Ischemia was achieved by bilaterally occluding both kidneys with a microvascular clamp for 45 min and verified visually by a change in tissue color. After post-reperfusion, urine samples were collected at 9, 24, and 48 hr intervals. Urinary excretion of protein-based biomarkers was measured by Western blot analysis. In cisplatin-treated rats, mild histopathologic alterations were noted at day 1 which became severe at day 3. Blood urea nitrogen (BUN) and serum creatinine (SCr) levels were significantly increased at day 3. Levels of urinary excretion of SBP1, neutrophil gelatinase-associated lipocalin (NGAL), and a tissue inhibitor of metalloproteinase-1 (TIMP-1) were markedly elevated at day 3 and 5 following drug treatment. In the vehicle-treated I/R group, serum levels of BUN and SCr and AST activity were significantly increased compared to sham. Urinary excretion of SBP1 and NGAL rose markedly following I/R. The urinary levels of SBP1, NGAL, TIMP-1, and KIM-1 proteins excreted by AKI patients and normal subjects were compared. Among these proteins, a marked rise in SBP1 was observed in urine of patients with AKI compared to normal subjects. Based upon receiver-operator curves (ROC), SBP1 displayed a higher area under the curve (AUC) scores than levels of SCr, BUN, total protein, and glucose. In particular, SBP1 protein was readily detected in small amounts of urine without purification. Data thus indicate that urinary excretion of SBP1 may be useful as a reliable biomarker for early diagnosis of AKI in patients.
- Published
- 2017
38. Highly Eribulin-resistant KBV20C Oral Cancer Cells Can Be Sensitized by Co-treatment with the Third-generation P-Glycoprotein Inhibitor, Elacridar, at a Low Dose
- Author
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Yu Jin Park, Ji-Yeon Son, Byung-Mu Lee, Hyung Sik Kim, and Sungpil Yoon
- Subjects
0301 basic medicine ,Eribulin Mesylate ,Cancer Research ,Pharmacology ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Cell Line, Tumor ,Tetrahydroisoquinolines ,medicine ,Humans ,Mitotane ,ATP Binding Cassette Transporter, Subfamily B, Member 1 ,Furans ,P-glycoprotein Inhibitor ,Sulindac ,Dose-Response Relationship, Drug ,Chemistry ,Drug Synergism ,General Medicine ,Ketones ,Tesmilifene ,030104 developmental biology ,Oncology ,Drug Resistance, Neoplasm ,030220 oncology & carcinogenesis ,Cancer cell ,Verapamil ,Acridines ,Mouth Neoplasms ,medicine.drug ,Eribulin - Abstract
Background/aim Eribulin mesylate, also called Halaven® (HAL), was recently developed as a microtubule-targeting drug and is used in the clinic for resistant or metastatic cancer. Previously, we showed that P-glycoprotein (P-gp)-overexpressing KBV20C oral cancer cells are highly resistant to HAL compared to sensitive KB cells. This qualitative study was designed to identify specific P-gp inhibitors that increase the sensitivity of highly resistant cancer cells to HAL. Materials and methods In order to identify functional P-gp inhibitors, HAL-treated KBV20C cells were co-treated with P-gp inhibitors, verapamil, elacridar, cyclosporine A, mitotane, piperine, fumagillin, curcumin, indomethacin, probenecid, sulindac, tesmilifene, and C-4. We then evaluated which P-gp inhibitors required a low dose to sensitize KBV20C cells to HAL. We also determined whether a low dose of a P-gp inhibitor could inhibit P-gp efflux pumping. Results We found that cyclosporine A sensitized HAL-treated KBV20C cells at a low dose, whereas verapamil, another first-generation P-gp inhibitor, required a dose that was nearly 10-fold higher. We also found that the natural products, piperine and mitotane, sensitized KBV20C cells to HAL co-treatment. Interestingly, we found that elacridar, a third-generation P-gp inhibitor, sensitized HAL-treated cells at a low dose. Elacridar required approximately a 500-fold lower dose than that of verapamil to exert a similar effect. All inhibitors showed P-gp inhibitory activity that correlated with sensitivity to HAL. Conclusion These results suggest that highly HAL-resistant cancer cells can be sensitized with cyclosporine A or elacridar, specific P-gp inhibitors that exert their effects at a low dose. These findings provide important information regarding the sensitization of highly HAL-resistant cells with selective P-gp inhibitors and indicate that elacridar may be used to treat such highly HAL-resistant cancer cells.
- Published
- 2017
39. EW-7197, a Novel ALK-5 Kinase Inhibitor, Potently Inhibits Breast to Lung Metastasis
- Author
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Ji Yeon Son, Seung Won Kim, Sol-Ji Kim, Seon Joo Lee, Dae-Kee Kim, Sang-A Park, Min-Jin Kim, Yhun Yhong Sheen, Soyeon Park, and Jeong-Seok Nam
- Subjects
Cancer Research ,medicine.medical_specialty ,Lung Neoplasms ,Receptor, Transforming Growth Factor-beta Type I ,Breast Neoplasms ,SMAD ,Protein Serine-Threonine Kinases ,Mice ,Random Allocation ,Cell Line, Tumor ,Internal medicine ,medicine ,Animals ,Humans ,Cytotoxic T cell ,Protein Kinase Inhibitors ,Mice, Inbred BALB C ,Aniline Compounds ,biology ,Kinase ,Chemistry ,Mouse mammary tumor virus ,Cancer ,Cell migration ,Triazoles ,biology.organism_classification ,medicine.disease ,Xenograft Model Antitumor Assays ,Endocrinology ,Oncology ,Tumor progression ,Cancer research ,Female ,Receptors, Transforming Growth Factor beta ,Signal Transduction ,Transforming growth factor - Abstract
Advanced tumors produce an excessive amount of transforming growth factor β (TGFβ), which promotes tumor progression at late stages of malignancy. The purpose of this study was to develop anti-TGFβ therapeutics for cancer. We synthesized a novel small-molecule TGFβ receptor I kinase (activin receptor–like kinase 5) inhibitor termed N-[[4-([1,2,4]triazolo[1,5-a]pyridin-6-yl)-5-(6-methylpyridin-2-yl)-1H-imidazol-2-yl]methyl]-2-fluoroaniline (EW-7197), and we investigated its potential antimetastatic efficacy in mouse mammary tumor virus (MMTV)/c-Neu mice and 4T1 orthotopic–grafted mice. EW-7197 inhibited Smad/TGFβ signaling, cell migration, invasion, and lung metastasis in MMTV/c-Neu mice and 4T1 orthotopic–grafted mice. EW-7197 also inhibited the epithelial-to-mesenchymal transition (EMT) in both TGFβ-treated breast cancer cells and 4T1 orthotopic–grafted mice. Furthermore, EW-7197 enhanced cytotoxic T lymphocyte activity in 4T1 orthotopic–grafted mice and increased the survival time of 4T1-Luc and 4T1 breast tumor–bearing mice. In summary, EW-7197 showed potent in vivo antimetastatic activity, indicating its potential for use as an anticancer therapy. Mol Cancer Ther; 13(7); 1704–16. ©2014 AACR.
- Published
- 2014
40. Evaluation of Renal Toxicity by Combination Exposure to Melamine and Cyanuric Acid in Male Sprague-Dawley Rats
- Author
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Tae Cheon Jeong, Hyung Sik Kim, Hyun Jung Lim, Ji Yeon Son, Yoon Jong Kang, Kyeong Seok Kim, Sung Kwang Lim, Byung Mu Lee, Kyu Hyuck Chung, Tae Hyung Kim, and Dal Woong Choi
- Subjects
Health, Toxicology and Mutagenesis ,Pharmacology ,Toxicology ,Nephrotoxicity ,chemistry.chemical_compound ,Chronic toxicity ,hemic and lymphatic diseases ,Mixture ,Medicine ,neoplasms ,Blood urea nitrogen ,Melamine ,Creatinine ,Kidney ,business.industry ,Cyanuric acid ,Articles ,carbohydrates (lipids) ,medicine.anatomical_structure ,chemistry ,Toxicity ,business - Abstract
Melamine-induced nephrotoxicity is closely associated with crystal formation in the kidney caused by combined exposure to melamine (Mel) and cyanuric acid (CA). However, there are few dosage-finding studies for toxicological evaluation of chronic co-exposure to Mel and CA. The objective of this study was to investigate the possible mechanism by which a Mel and CA mixture lead to renal toxicity in rats. Mel and CA were co-administered to rats via oral gavage for 50 days. Nephrotoxicity was determined by measuring blood urea nitrogen (BUN) and serum creatinine (sCr) levels. Relative kidney weights were significantly increased in rats after co-exposure to Mel+CA (63/6.3 or 630/6.3 mg/kg) mixtures. BUN and sCr levels were significantly increased after Mel and CA co-exposure. Taken together, significant increase in KIM-1, NGAL, and calbindin levels were observed in the urine of rats exposed to Mel+CA (63/6.3 or 630/6.3 mg/kg) compared with the corresponding control group. Histological analysis revealed epithelial degeneration and necrotic cell death in the proximal tubules of the kidney after co-exposure to Mel+CA (63/6.3 or 630/6.3 mg/kg). Our data suggest that Mel-mediated renal toxicity may be influenced by CA concentrations in Mel-contaminated milk or foods.
- Published
- 2014
41. Multi agent 3D printer and robot system for mass personalization faas platform
- Author
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Hyun Kim, Hyonyoung Han, Heechul Bae, Ji-Yeon Son, and Hyun-Chul Kang
- Subjects
0209 industrial biotechnology ,Service (systems architecture) ,business.industry ,Computer science ,Multi-agent system ,020208 electrical & electronic engineering ,ComputerApplications_COMPUTERSINOTHERSYSTEMS ,02 engineering and technology ,Personalization ,3d printer ,020901 industrial engineering & automation ,Robotic systems ,Embedded system ,Server ,0202 electrical engineering, electronic engineering, information engineering ,Factory (object-oriented programming) ,Robot ,business - Abstract
Factory as a Service is a mass personalization service platform. The system consists of three platforms; portal cloud system, manufacturing execution platform and manufacturing operation platform including IoT based manufacturing instruments called micro smart factory. The smart factory include almost all the manufacturing process instruments; manufacturing instrument such as 3D printer, post-processing instruments, inspection instrument, transfer instrument, and packing instruments. The smart factory works in multi agent system based on IoT middleware. Each instrument transfers fundamental information of product part and operation command. This system has continued to be evolved to be autonomous control Micro Smart Factory including intelligent instruments.
- Published
- 2016
42. ICT convergence-based application service development to support the re-configurability of door trim assembly line
- Author
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Ji Yeon Son, Hyun Jong Kim, Young Ae Jeon, and Yong Kwi Lee
- Subjects
Scheme (programming language) ,0209 industrial biotechnology ,Service (systems architecture) ,Computer science ,business.industry ,Control reconfiguration ,02 engineering and technology ,Trim ,Product (business) ,020901 industrial engineering & automation ,Information and Communications Technology ,Server ,Embedded system ,0202 electrical engineering, electronic engineering, information engineering ,Production (economics) ,020201 artificial intelligence & image processing ,business ,computer ,computer.programming_language - Abstract
In this paper, We propose the ICT convergence-based application service to support the re-configurability of door trim assembly line. There is a need for a scheme capable of producing a variety of product models in an assembly line because recent car market has been converted from massive production massive consumption to small production small consumption. So, we develop an application service for reconfiguration considering the complexity and recyclability of assembly line.
- Published
- 2016
43. Protective Effects of Dendropanax morbifera against Cisplatin-Induced Nephrotoxicity without Altering Chemotherapeutic Efficacy
- Author
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Kyeong Seok Kim, Hae Ri Kim, Kwang Youl Lee, Hyung Sik Kim, Jae Hyeon Park, In Su Kim, Ji Yeon Son, Byung Mu Lee, Jong Hwan Kwak, Su Hyun Lee, Da Eun Lee, and Ji-Su Kim
- Subjects
inorganic chemicals ,0301 basic medicine ,Physiology ,medicine.medical_treatment ,Clinical Biochemistry ,Dendropanax morbifera ,cisplatin ,xenograft model ,Renal function ,Pharmacology ,urologic and male genital diseases ,chemotherapy ,medicine.disease_cause ,Biochemistry ,Article ,Nephrotoxicity ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Medicine ,neoplasms ,Molecular Biology ,Cisplatin ,Creatinine ,Chemotherapy ,Kidney ,urogenital system ,business.industry ,lcsh:RM1-950 ,Acute kidney injury ,Cell Biology ,medicine.disease ,female genital diseases and pregnancy complications ,antioxidants ,lcsh:Therapeutics. Pharmacology ,030104 developmental biology ,medicine.anatomical_structure ,chemistry ,030220 oncology & carcinogenesis ,renoprotective effect ,business ,Oxidative stress ,medicine.drug - Abstract
Use of the chemotherapeutic agent cisplatin (CDDP) in cancer patients is limited by the occurrence of acute kidney injury (AKI), however, no protective therapy is available. We aimed to investigate the renoprotective effects of Dendropanax morbifera water extract (DM) on CDDP-induced AKI. Male Sprague-Dawley rats (six animals/group) received: Vehicle (control), CDDP (6 mg/kg, intraperitoneally (i.p.), DM (25 mg/kg, oral), or DM + CDDP injection. CDDP treatment significantly increased blood urea nitrogen (BUN), serum creatinine (sCr), and pro-inflammatory cytokines (IL-6 and TNF-&alpha, ), and severely damaged the kidney architecture. Urinary excretion of protein-based AKI biomarkers also increased in the CDDP-treated group. In contrast, DM ameliorated CDDP-induced AKI biomarkers. It markedly protected against CDDP-induced oxidative stress by increasing the activity of endogenous antioxidants and reducing the levels of pro-inflammatory cytokines (IL-6 and TNF-&alpha, ). The protective effect of DM in the proximal tubules was evident upon histopathological examination. In a tumor xenograft model, administration of DM enhanced the chemotherapeutic activity of CDDP and exhibited renoprotective effects against CDDP-induced nephrotoxicity without altering chemotherapeutic efficacy. Our data demonstrate that DM may be an adjuvant therapy with CDDP in solid tumor patients to preserve renal function.
- Published
- 2019
44. RAFD: Resource-aware fault diagnosis system for home environment with smart devices
- Author
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Ji Hyun Lee, Younghee Lee, Ji-Yeon Son, Jun-Hee Park, and JeuYoung Kim
- Subjects
Engineering ,Home environment ,business.industry ,Real-time computing ,Tracing ,Root cause ,Fault management ,Home automation ,High availability ,Media Technology ,Snapshot (computer storage) ,Electrical and Electronic Engineering ,Latency (engineering) ,business - Abstract
With recent advancement in technologies used at home, smart home environment allows various resources such as device, network, or content to be connected to one another. Their configurations are changed by dynamic bindings at any time. In this smart home environment, a minor problem in a resource can trigger serious failures in home network services by causing multiple faults to the related resources simultaneously. To solve this problem, it is essential to analyze the dependency between resources and also to diagnose home network faults autonomously. This paper proposes the effective fault diagnosis system based on resource relation map which is dynamically constructed by information convergence model of heterogeneous home resources. The proposed system provides the tracing method for finding the root cause of a fault using the resource relation map. The resource relation map represents the snapshot of home situations at the given time. The proposed fault diagnosis method allows building cost effective remote maintenance system with high availability and manageability by tracing the fault cause along the dependency between resources using graph-style resource relation map as if humans trace the cause of problem. In addition, it can contribute to realize an autonomic fault management system for smart home. In this paper, the prototype of the proposed system is implemented and evaluated for performance in accuracy and latency of fault diagnosis in a real environment. The experimental results show that the proposed system, especially with the suggested back tracing diagnosis system, yields remarkable performance for home network fault diagnosis.
- Published
- 2012
45. Evaluation of 111In-labeled macrocyclic chelator-amino acid derivatives for cancer imaging
- Author
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Dinesh K. Shetty, Mi Kyung Hong, Jong Jin Lee, Yun Sang Lee, Young Joo Kim, Jae Min Jeong, Ji Yeon Son, and Sang Eun Kim
- Subjects
Cancer Research ,Biodistribution ,Transplantation, Heterologous ,Cell ,Mice, Nude ,Heterocyclic Compounds, 1-Ring ,Mice ,chemistry.chemical_compound ,Cell Line, Tumor ,Neoplasms ,Spect imaging ,medicine ,Animals ,Humans ,DOTA ,Tissue Distribution ,Radiology, Nuclear Medicine and imaging ,Amino acid transporter ,Amino Acids ,Chelating Agents ,Tomography, Emission-Computed, Single-Photon ,chemistry.chemical_classification ,Aminobutyrates ,Indium Radioisotopes ,Tryptophan ,Glioma ,In vitro ,Amino acid ,medicine.anatomical_structure ,chemistry ,Biochemistry ,Molecular Medicine ,Radiopharmaceuticals - Abstract
Purpose We evaluated new 111 In-labeled amino acid derivatives, in which the amino acids are conjugated with1,4,7,10-tetra-azacyclododecane-1,4,7,10-tetraacetic acid (DOTA), 1,4,7,10-tetraazacyclododecane-1,7-diacetic acid (DO2A) or 1,4,7,10-tetraazacyclododecane-1,4,7-triacetic acid (DO3A). Methods DOTA-aminoalanine (DOTA-A), DOTA-aminohomoalanine (DOTA-H), DOTA-lysine (DOTA-L), DO2A-alanine (DO2A-A), DO3A-alanine (DO3A-A) and DO3A-homoalanine (DO3A-H) were labeled with 111 In. In vitro cell uptake assays were performed usingHep3B (a human hepatoma cell line), CT26 (a mouse colon cancer cell line) and U87MG (a human glioma cell line). In vitro cell uptake inhibition assays were performed using U87MG and 111 In-DO3A-H. U87MG bearing xenografted mice were subject to biodistribution, SPECT imaging, autoradiography, and immunohistochemistry studies. Results Of the amino acid derivatives and cell lines examined, U87MG and 111 In-DO3A-H showed highest uptake in vitro. This uptake was blocked by 2-aminobicyclo-[2,2,1] heptane-2-carboxylic acid (BCH) and by tryptophan. 111 In-DO3A-HSPECT imaging of U87MG bearing xenografted mice visualized tumors (mean tumor-to-muscle ratio 3.16±0.74). Autoradiography and immunohistochemistry revealed that 111 In-DO3A-H uptake matched L-type amino acid transporter 1 expression. Conclusion Tumor uptake was successfully imaged using 111 In-DO3A-H in U87MG bearing xenografted mice. 111 In-DO3A-H appears to be useful for imaging tumors expressing L-type amino acid transporter.
- Published
- 2012
46. Pyruvate Kinase M2: A Novel Biomarker for the Early Detection of Acute Kidney Injury
- Author
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Hyung Sik Kim, Byung Mu Lee, Ye Rim Kang, Aree Moon, Ho Sub Song, Ji Hye An, Ji Yeon Son, Ji Hoon Kwon, Sun Young Kim, and Ji Hyun Cheon
- Subjects
0301 basic medicine ,medicine.medical_specialty ,Health, Toxicology and Mutagenesis ,Disease ,PKM2 ,Toxicology ,Bioinformatics ,urologic and male genital diseases ,Nephrotoxicity ,03 medical and health sciences ,chemistry.chemical_compound ,medicine ,Intensive care medicine ,Blood urea nitrogen ,Pyruvate kinase M2 ,Creatinine ,business.industry ,urogenital system ,Acute kidney injury ,Special Issue Article ,Biomarker ,medicine.disease ,female genital diseases and pregnancy complications ,030104 developmental biology ,chemistry ,Biomarker (medicine) ,business ,Pyruvate kinase - Abstract
The identification of biomarkers for the early detection of acute kidney injury (AKI) is clinically important. Acute kidney injury (AKI) in critically ill patients is closely associated with increased morbidity and mortality. Conventional biomarkers, such as serum creatinine (SCr) and blood urea nitrogen (BUN), are frequently used to diagnose AKI. However, these biomarkers increase only after significant structural damage has occurred. Recent efforts have focused on identification and validation of new noninvasive biomarkers for the early detection of AKI, prior to extensive structural damage. Furthermore, AKI biomarkers can provide valuable insight into the molecular mechanisms of this complex and heterogeneous disease. Our previous study suggested that pyruvate kinase M2 (PKM2), which is excreted in the urine, is a sensitive biomarker for nephrotoxicity. To appropriately and optimally utilize PKM2 as a biomarker for AKI requires its complete characterization. This review highlights the major studies that have addressed the diagnostic and prognostic predictive power of biomarkers for AKI and assesses the potential usage of PKM2 as an early biomarker for AKI. We summarize the current state of knowledge regarding the role of biomarkers and the molecular and cellular mechanisms of AKI. This review will elucidate the biological basis of specific biomarkers that will contribute to improving the early detection and diagnosis of AKI.
- Published
- 2015
47. Resource-aware smart home management system by constructing resource relation graph
- Author
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Kyeong-Deok Moon, Jun-Hee Park, Younghee Lee, and Ji-Yeon Son
- Subjects
Service (systems architecture) ,Ubiquitous computing ,Relation (database) ,business.industry ,Computer science ,Computer security ,computer.software_genre ,Fault management ,Resource (project management) ,Home automation ,Management system ,Media Technology ,Resource management ,Electrical and Electronic Engineering ,business ,computer - Abstract
With the advent of smart home devices including Smart TV or smart appliances, home becomes smarter. Smart home is a complex environment where heterogeneous smart devices and appliances are connected to each other to provide various smart services. As the home environments become smarter and more complex, the need for an effective managing mechanism with minimum user's intervention is growing. However, just only putting together all the individual information of each resource is not enough, since it is very hard to catch out overall home situation without the relation information between the managed resources. In this paper, we propose a resource-aware management system with hierarchical smart home resource model, using the home context information which defines the information convergence model of heterogeneous home resources and builds a home knowledge by constructing a resource relation graph. The proposed system can accelerate deployment of advanced future smart home service features such as context-aware dynamic service composition, autonomous fault management systems. In addition, it allows cost effective remote maintenance system with highly convenient manageability. We implemented the prototype system of our proposed architecture and evaluated the performance on the query response time of home resources and relations in a real environment. Results showed that it has acceptable performance for navigating or control of home information and excellent improvements in terms of relation based search. We anticipate that the proposed system will bring the huge benefits not only to the consumers, home network service providers but also to the home network maintenance companies in terms of ease of management, and diagnostic effectiveness.
- Published
- 2011
48. Identification of sensitive biomarker, 3-indoxyl sulfate, to detecting acute kidney injury
- Author
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Hyung Sik Kim, Ji Yeon Son, and Amit Kundu
- Subjects
0301 basic medicine ,business.industry ,Acute kidney injury ,General Medicine ,Toxicology ,medicine.disease ,03 medical and health sciences ,030104 developmental biology ,Cancer research ,medicine ,Biomarker (medicine) ,Indoxyl Sulfate ,Identification (biology) ,business - Published
- 2017
49. Smart home web of objects-based IoT management model and methods for home data mining
- Author
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Hark-Jin Lee, Jun-Hee Park, Ji-Yeon Son, and Jeu Young Kim
- Subjects
Computer science ,business.industry ,Smart device ,Management model ,Data modeling ,Domain (software engineering) ,law.invention ,World Wide Web ,Home automation ,Analytics ,Mobile phone ,law ,business ,Internet of Things - Abstract
Nowadays, much research in recent years has focused on IoT (Internet of Things). The home domain is the most important research area of IoT, because there expected accounts of home smart device for over 40 percent of connected device excepting mobile phone. Furthermore, enormous data is generated by home smart device. There is growing concern, but the previous works didn't address enough to manage and analyze home data. The purpose of this study is to describe and examine to manage the aggregated home IoT data based on SWO (Smart home Web of Objects), and SWO analytics platform. We shows the implementation of SWO analytics platform and a case study using real data from smart metering devices for analysis of appliance usage patterns.
- Published
- 2015
50. A New Histone Deacetylase Inhibitor, MHY219, Inhibits the Migration of Human Prostate Cancer Cells via HDAC1
- Author
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Ji Hae Ahn, Soma Kundu, Umasankar De, Ji Yeon Son, Nabanita Patra, Hyung Ryoung Moon, Byung Mu Lee, Mee Young Ahn, Jung Hyun Yoon, and Hyung Sik Kim
- Subjects
Pharmacology ,Prostate cancer ,medicine.drug_class ,Chemistry ,Histone deacetylase inhibitor ,medicine.disease ,urologic and male genital diseases ,Biochemistry ,Molecular biology ,HDAC1 ,DU145 ,HDAC inhibitor ,Drug Discovery ,Cancer cell ,LNCaP ,medicine ,Cancer research ,Molecular Medicine ,Original Article ,MHY219 ,Histone deacetylase ,MMPs ,Cytotoxicity ,Migration - Abstract
Histone deacetylase (HDAC) inhibitors are considered novel agents for cancer chemotherapy. We previously investigated MHY219, a new HDAC inhibitor, and its potent anticancer activity in human prostate cancer cells. In the present study, we evaluated MHY219 molecular mechanisms involved in the regulation of prostate cancer cell migration. Similar to suberanilohydroxamic acid (SAHA), MHY219 inhibited HDAC1 enzyme activity in a dose-dependent manner. MHY219 cytotoxicity was higher in LNCaP (IC50=0.67 μM) than in DU145 cells (IC50=1.10 μM) and PC3 cells (IC50=5.60 μM) after 48 h of treatment. MHY219 significantly inhibited the HDAC1 protein levels in LNCaP and DU145 cells at high concentrations. However, inhibitory effects of MHY219 on HDAC proteins levels varied based on the cell type. MHY219 significantly inhibited LNCaP and DU145 cells migration by down-regulation of matrix metalloprotease-1 (MMP-1) and MMP-2 and induction of tissue inhibitor of metalloproteinases-1 (TIMP-1). These results suggest that MHY219 may potentially be used as an anticancer agent to block cancer cell migration through the repression of MMP-1 and MMP-2, which is related to the reduction of HDAC1.
- Published
- 2015
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