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1. Additional file 1 of First time β-farnesene production by the versatile bacterium Cupriavidus necator

Author

Milker, Sofia and Holtmann, Dirk

Abstract

Additional file 1. The construction of the plasmids to produce farnesene is described in detail. In addition, the MVA pathway (plasmid pKRTK3) coupled with the β-farnesene synthase (plasmid pBBR1c_farn) expression is presented. The calculations of the oxygen and carbon dioxide transfer rates (OTR/CTR) via the gas-balancing method is shown. Furthermore, the β-farnesene calibration with caffeine as internal standard is described.

Published
2021
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