1. Biomarkers of inflammation and oxidative stress in smokers and severe chronic obstructive pulmonary disease patients
- Author
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Mohammed Asimuddin, Mohd. Soheb Ansari, Kaiser Jamil, and Gutta Vijaylakshmi
- Subjects
medicine.medical_specialty ,Antioxidant ,medicine.medical_treatment ,Inflammation ,medicine.disease_cause ,Proinflammatory cytokine ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,medicine ,General Environmental Science ,chemistry.chemical_classification ,COPD ,business.industry ,Glutathione peroxidase ,General Engineering ,Interleukin ,medicine.disease ,Endocrinology ,Pulmonology ,030228 respiratory system ,chemistry ,General Earth and Planetary Sciences ,medicine.symptom ,business ,Oxidative stress ,030215 immunology - Abstract
Identification of biomarkers for the novel therapeutics targets of Chronic obstructive pulmonary disease (COPD) is an important area of current research. In this study, the level of inflammatory cytokines was investigated and correlated these levels with erythrocytic antioxidant activities in COPD patients with smokers and without smokers and parameters of severity.Plasma levels of Interleukin (IL)-6, IL-8 and IL-10 concentrations were assayed by means of Enzyme-linked Immunosorbent Assay (ELISA) and erythrocytic glutathione-s-transferase (GST) and glutathione peroxidase (GPx) activities were estimated by spectrophotometric method.Both IL-6 and IL-8 plasma levels showed a statistically significantly higher in COPD patients as compared to healthy controls (p0.05) and GPx activities (r= - 0.080, p>0.05). Similarly IL-8 was also found negative association with GST (r= - 0.260, p>0.05) and GPx activities (r= - 0.268, p>0.05). Whereas, a significant positive association was observed between IL-10 and erythrocytic GST (r= 0.494, p In conclusion, Plasma levels of inflammatory cytokines IL-6 and IL-8 are related with severity of COPD and IL-10 and oxidative stress markers GST and GPx are co-dependent and strongly interrelated processes and may be used as a potential marker for the evaluation of COPD.
- Published
- 2020