77 results on '"Muhammad Ishtiaq Ali"'
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2. Changes of Permeability and Porosity of Tiefa Anthracite after Treatment with an Acetogenic Bacterium Clostridium sp
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Yue Cao, Huan He, Di Zhan, Hua-Zhou Huang, Yong Zhang, Bo Fu, Zhi-Min Xu, Muhammad Ishtiaq Ali, Fang-Jing Liu, Xiu-Xiang Tao, and Huang Zaixing
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Earth and Planetary Sciences (miscellaneous) ,Environmental Chemistry ,Microbiology ,General Environmental Science - Published
- 2022
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3. Accompanying effects of sewage sludge and pine needle biochar with selected organic additives on the soil and plant variables
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Bushra Fatima, Farhana Bibi, Muhammad Ishtiaq Ali, Jeremy Woods, Mushtaq Ahmad, Muhammad Mubashir, Mohd Shariq Khan, Awais Bokhari, and Kuan Shiong Khoo
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Technology ,POTENTIAL APPLICATIONS ,IMPACT ,Environmental Sciences & Ecology ,Solid Waste ,PYROLYSIS TEMPERATURE ,Soil ,Engineering ,Crop rotation ,Organic additives ,Microalgae ,FERTILITY ,RETENTION ,FEEDSTOCK TYPE ,AMENDMENT ,Fertilizers ,Waste Management and Disposal ,EMISSIONS ,Science & Technology ,Sewage ,Engineering, Environmental ,Carbon ,Manure ,Biochar ,0907 Environmental Engineering ,Charcoal ,CROP PRODUCTION ,GROWTH ,Life Sciences & Biomedicine ,Chemical and structural characterization ,Environmental Sciences - Abstract
The effects of synthetic fertilizer and nutrient leaching are causing serious problems impacting soil function and its fertility. Mitigation of nutrient leaching and use of chemical fertilizer is crucial as fertile land adds up sustainability to climate changes. Biochar produced from agricultural bio-waste and municipal solid waste has been used for crop production and when applied in combination with organic nutrients may support mitigation of nutrient loss and adverse effects of chemical fertilizers. Different types of biochar and their application for soil enhancement have been observed, pine needle and sewage sludge derived low-temperature biochar along with compost, organic fertilizer in the form of manure and microalgal biomass may interact with soil chemistry and plant growth to impact nutrient loss and compensate the hazardous effect of chemical fertilizer, but it has not been investigated yet. This present study elaborates application of sewage sludge and pine needle biochar produced at 400 °C in an application rate of 5 % w/w and 10 t h-1 in combination with compost, manure and microalgal biomasses of Closteriopsis acicularis (BM1) and Tetradesmus nygaardi (BM2) on the growth of Chickpea (Cicer arietinum) and Fenugreek (Trigonella foenum-graecum) crop assessed in a pot experiment over a two crop (Chickpea - Fenugreek) cycle in Pakistan. Results depict that the pine needle biochar with additives has increased plant height by 104.1 ± 2.76 cm and fresh biomass by 49.9 ± 1.02 g, buffered the soil pH to 6.5 for optimum growth of crops and enhance carbon retention by 36 %. This study highlights the valorization of sewage sludge and pine needle into biochar and the effect of biochar augmentation, its impact on soil nutrients and plant biomass enhancement. The greener approach also mitigates and helps in the sustainable management of solid wastes.
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- 2022
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4. Response of mixed bacterial culture towards dibenzothiophene desulfurization under the influence of surfactants and microscopically ( <scp>SEM</scp> and <scp>TEM</scp> ) characterized magnetic <scp> Fe 3 O 4 </scp> nanoparticles
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Javed Khan, Muhammad Ishtiaq Ali, Asif Jamal, Mushtaq Ahmad, Jahangir Khan Achakzai, and Muhammad Zafar
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Surface-Active Agents ,Medical Laboratory Technology ,Coal ,Histology ,Magnetic Phenomena ,Polysorbates ,Nanoparticles ,Anatomy ,Instrumentation - Abstract
Excessive emission of sulfur dioxides from the combustion of coal and other fossil fuels for thermal and industrial purposes has been associated with serious environmental hazards. Biodesulfisation (BDS) can be an effective approach for reducing the impact of toxic gases to its inbuilt operational feasibility under ambient environmental conditions. In the present research, two strategies for BDS of a standard organosulfur compound such as dibenzothiophene (DBT) were investigated under laboratory conditions. In the first treatment, the role of different surfactants such as Tween-20, Tween-80, SDS, and EDTA on the desulfurization of DBT was investigated by the application of bacterial consortium IQMJ-5. In the second treatment, Iron oxidenanoparticles were synthesized and immobilized on the surface of bacteria cells. Shake flask experiments were conducted with immobilized cells, surfactant amended immobilized cells, and control or noncoated cells. Among different surfactant treatments, Tween-80 was found to be the most effective surfactant, showing maximum desulfurization activity at a concentration of 5 g/L. The transmission electron microscopy and X-ray diffraction analysis indicated that produced nanoparticles were spherical in shape with a size of about 46 nm and had a stoichiometric ratio of 55.85% and 44.15% between O and Fe, respectively. The nanoparticle treatment enhanced the DBT desulfurization process up to 11.37% as compared to the control, specifically when immobilized cells were used. Therefore, it was concluded that nanoparticles treatments with immobilization of the bacterial cells enhanced the desulfurization rate of DBT under ambient reaction conditions and provide a sustainable alternative for commercial coal BDS.
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- 2022
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5. Effect of Fe3O4 coated bacterial consortium IQMJ-5 on the desulfurization of the organosulfur portion of Pakistani coal and its chemical characteristics
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Javed Khan, Muhammad Ishtiaq Ali, Jahangir Khan Achakzai, Asif Jamal, Siraj Uddin, and Rabia Liaquat
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Coal is an abundant and inexpensive source of energy with immense application in different industrial sectors. However, its extensive utilization has resulted in several kinds of sulfur-related environmental issues. To tackle such issues, indigenously isolated Fe3O4-coated bacterial consortium IQMJ-5 cells were applied for the desulfurization of a Pakistani coal. The coated cells were allowed to desulfurize the coal in a shake flask experiment. To assess the desulfurization capabilities, both the pre- and post-desulfurized coal were characterized by different chemical and analytical techniques. The total sulfur content identified in the coal sample was about 4.50%. The results of the analysis showed that after treatment an increase in the carbon content and heating value of the coal sample was detected. Moreover, an amount of about 54.46% and 66.6% of organic and total sulfur, respectively were removed after biodesulfurization. The obtained results clearly suggest that the consortium can be a potential candidate for reducing sulfur-related environmental pollutants of fossil fuels at a more advanced commercial scale.
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- 2023
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6. The succession of microorganisms and organics in the process of methane generation by co‐degradation of anthracite and rice straw
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Zhigang Li, Jiaqi Cheng, Kaixin Duan, Yatong Cheng, Hongguang Guo, Zaixing Huang, Michael Urynowicz, and Muhammad Ishtiaq Ali
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Fuel Technology ,Nuclear Energy and Engineering ,Renewable Energy, Sustainability and the Environment ,Energy Engineering and Power Technology - Published
- 2022
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7. Corrigendum: Contaminants from a former Croatian coal sludge dictate the structure of microbiota in the estuarine (Raša Bay) sediment and soil
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Weiting Zhang, Qianyun Mo, Zaixing Huang, Muhammad Adnan Sabar, Gordana Medunić, Tatjana Ivošević, Huan He, Michael Urynowicz, Fang-Jing Liu, Hongguang Guo, Rizwan Haider, Muhammad Ishtiaq Ali, and Asif Jamal
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Microbiology (medical) ,Microbiology - Published
- 2023
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8. Role of Biosurfactants in Agriculture Management
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Asif Jamal, Muhammad Ishtiaq Ali, Malik Badshah, and Aetsam Bin Masood
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- 2023
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9. Deciphering role of technical bioprocess parameters for bioethanol production using microalgae
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Muhammad Zafar, Bashir Ahmad, Asif Jamal, Humaira Yasmin, Atef F. Ahmed, Mushtaq Ahmad, Farhana Bibi, B. N. Samra, Muhammad Ishtiaq Ali, and Mohammad S. AL-Harbi
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Optimization ,QH301-705.5 ,RSM ,food and beverages ,Biomass ,Bioethanol ,Bacterial growth ,Raw material ,Phosphate ,chemistry.chemical_compound ,chemistry ,Productivity (ecology) ,Biofuel ,Biomass productivity ,Microalgae ,Original Article ,Ethanol fuel ,Food science ,Biology (General) ,Bioprocess ,General Agricultural and Biological Sciences ,Specific growth rate - Abstract
Microalgae biomass is considered an important feedstock for biofuels and other bioactive compounds due to its faster growth rate, high biomass production and high biomolecules accumulation over first and second-generation feedstock. This research aimed to maximize the specific growth rate of fresh water green microalgae Closteriopsis acicularis, a member of family Chlorellaceae under the effect of pH and phosphate concentration to attain enhanced biomass productivity. This study investigates the individual and cumulative effect of phosphate concentration and pH on specific growth characteristics of Closteriopsis acicularis in autotrophic mode of cultivation for bioethanol production. Central-Composite Design (CCD) strategy and Response Surface Methodology (RSM) was used for the optimization of microalga growth and ethanol production under laboratory conditions. The results showed that high specific growth rate and biomass productivity of 0.342 day−1 and 0.497 g L−1 day−1 respectively, were achieved at high concentration of phosphate (0.115 g L−1) and pH (9) at 21st day of cultivation. The elemental composition of optimized biomass has shown enhanced elemental accumulation of certain macro (C, O, P) and micronutrients (Na, Mg, Al, K, Ca and Fe) except for nitrogen and sulfur. The Fourier transform infrared spectroscopic analysis has revealed spectral peaks and high absorbance in spectral range of carbohydrates, lipids and proteins, in optimized biomass. The carbohydrates content of optimized biomass was observed as 58%, with 29.3 g L−1 of fermentable sugars after acid catalyzed saccharification. The bioethanol yield was estimated as 51 % g ethanol/g glucose with maximum of 14.9 g/L of bioethanol production. In conclusion, it can be inferred that high specific growth rate and biomass productivity can be achieved by varying levels of phosphate concentration and pH during cultivation of Closteriopsis acicularis for improved yield of microbial growth, biomass and bioethanol production.
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- 2021
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10. Assessment of the dibenzothiophene desulfurization potential of indigenously isolated bacterial consortium IQMJ-5: A different approach to safeguard the environment
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Javed Khan, Muhammad Ishtiaq Ali, Asif Jamal, Jahangir Khan Achakzai, Jafir Hussain Shirazi, and Abdul Haleem
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Genetics ,General Medicine ,Molecular Biology ,Biochemistry ,Microbiology - Abstract
Biodesulfurization is emerging as a valuable technology for the desulfurization of dibenzothiophene (DBT) and its alkylated substitutes, which are otherwise regarded as refractory to other physical and chemical desulfurizing techniques. However, the technique is currently facing the issue of the nonavailability of an effective microbial isolate with the capability of increased desulfurizing rate and the ability to tackle the problem of product inhibition. Pure cultures belonging to different genera have been used at a large scale for the desulfurization of fossil fuels while studies on the desulfurization of fossil fuels employing microbial consortia are entirely missing. Herein, we isolated several kinds of dibenzothiophene desulfurizing bacterial consortia from hydrocarbon-contaminated soil samples by conventional enrichment technique. The outcomes of Gibb’s assay analysis showed that six isolates followed the “4S” pathway and converted DBT to 2-hydroxybiphenyl (2-HBP). Among the isolates, I5 showed maximum growth rate (0.0064 g dry cell weight L-1h-1) and desulfurization activity (about 77% as indicated by HPLC analysis) and was considered for further in-depth experimentation. The analysis of 16S rRNA by high throughput sequencing approach of the I5 isolate revealed five types of bacterial phyla including Proteobacteria, Bacteroidetes, Firmicutes, Patascibacteria, and Actinobacteria (in order of abundance). The isolate showed significant tolerance to the inhibitory effect of both 2-HBP and sulfate and maintained growth in the presence of even about 1.0 mM initial concentration of both products. This clearly suggests that the isolate can be an efficient candidate for improving the quality of the coal.
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- 2022
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11. Evaluating the use of unassimilated bio‐anode with different exposed surface areas for bioenergy production using solar‐powered microbial electrolysis cell
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Rabia Liaquat, Naseem Iqbal, Nadia Shahzad, Azhar Uddin, Muhammad Muddasar, Asif Hussain Khoja, Sami Ullah, Muhammad Ishtiaq Ali, and Ali Abdullah
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Fuel Technology ,Nuclear Energy and Engineering ,Waste management ,Renewable Energy, Sustainability and the Environment ,Bioenergy ,Chemistry ,Microbial electrolysis cell ,Energy Engineering and Power Technology ,Production (economics) ,Methane production ,Solar powered ,Hydrogen production ,Anode - Published
- 2021
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12. Biogenic methane generation from Vietnamese coal after pretreatment with hydrogen peroxide
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Rizwan Haider, Fang-Jing Liu, Quoc Hung Le, Ngoc Tran Han, Michael A. Urynowicz, Zaixing Huang, Mahmood Saleem, Lan Hoang, Huan He, Honggunag Guo, Muhammad Ishtiaq Ali, H. Sattar, and Kim Anh To
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Renewable Energy, Sustainability and the Environment ,Chemistry ,business.industry ,Vietnamese ,Energy Engineering and Power Technology ,language.human_language ,Methane ,chemistry.chemical_compound ,Fuel Technology ,Nuclear Energy and Engineering ,Environmental chemistry ,language ,Coal ,Hydrogen peroxide ,business - Published
- 2021
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13. Microbial and enzymatic degradation of PCBs from e-waste-contaminated sites: a review
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Foqia Khalid, Muhammad Ishtiaq Ali, Nadia Jamil, Muhammad Zaffar Hashmi, and Abdul Qadir
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Dehalococcoides ,Comamonas ,biology ,Chemistry ,Health, Toxicology and Mutagenesis ,food and beverages ,Environmental pollution ,General Medicine ,Dehalobacter ,010501 environmental sciences ,Biodegradation ,biology.organism_classification ,01 natural sciences ,Pollution ,Bioremediation ,Dioxygenase ,Environmental chemistry ,Environmental Chemistry ,Alcaligenes ,0105 earth and related environmental sciences - Abstract
Electronic waste is termed as e-waste and on recycling it produces environmental pollution. Among these e-waste pollutants, polychlorinated biphenyls (PCBs) are significantly important due to ubiquitous, organic in nature and serious health and environmental hazards. PCBs are used in different electrical equipment such as in transformers and capacitors for the purposes of exchange of heat and hydraulic fluids. Bioremediation is a reassuring technology for the elimination of the PCBs from the environment. In spite of their chemical stability, there are several microbes which can bio-transform or mineralize the PCBs aerobically or anaerobically. In this review paper, our objective was to summarize the information regarding PCB-degrading enzymes and microbes. The review suggested that the most proficient PCB degraders during anaerobic condition are Dehalobacter, Dehalococcoides, and Desulfitobacterium and in aerobic condition are Burkholderia, Achromobacter, Comamonas, Ralstonia, Pseudomonas, Bacillus, and Alcaligenes etc., showing the broadest substrate among bacterial strains. Enzymes found in soil such as dehydrogenases and fluorescein diacetate (FDA) esterases have the capability to breakdown PCBs. Biphenyl upper pathway involves four enzymes: dehydrogenase (bphB), multicomponent dioxygenase (bphA, E, F, and G), second dioxygenase (bphC), hydrolase, and (bphD). Biphenyl dioxygenase is considered as the foremost enzyme used for aerobic degradation of PCBs in metabolic pathway. It has been proved that several micro-organisms are responsible for the PCB metabolization. The review provides novel strategies for e-waste-contaminated soil management.
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- 2021
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14. Optimizing biocatalytic potential of Dipodascus australiensis M-2 for degrading lignin under laboratory conditions
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Saleha Parveen, Muhammad Ishtiaq Ali, Maryam Aslam, Irfan Ali, Asif Jamal, Mysoon M. Al-Ansari, Latifah Al-Humaid, Michael Urynowicz, and Zaixing Huang
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Paper ,Agar ,Biodegradation, Environmental ,Laccase ,Industrial Waste ,Alkalies ,Microbiology ,Lignin ,Dipodascus - Abstract
In present research, a potent fungal strain was isolated from paper mill effluent (black liquor) in order to investigate its potential for the biodegradation of lignin. Two step strategy was used to screen most efficient fungal strain having ability to growin MSM-black liquor medium and to degrade alkali lignin.The results of initial screening indicated that the strain M-2 produced comparatively higher ligninolytic zone on MSN agar plates supplemented with black liquor (BL) and alkali ligninase compared to the other isolates.The results of 18S rRNA gene sequencing revealed that strain M-2 showed ≥ 99% sequence homology with Dipodasceus australiansis.The process for the biodegradation of lignin was optimized using Taguchi Orthogonal Array design. Under optimized conditions of pH 9, 40 °C and 4% inoculum, a maximum of 89% lignin was degraded with 41% color reduction after 8 days of incubation period by Dipodasceus australiansis M-2. The pH and temperature were found to be significant terms with the p-values of 0.002 and 0.001 respectively. The laccase activity of the Dipodascus australiensis was found to be maximum of 1.511 U/mL. The HPLC analysis of lignin biodegradation indicated sharp transformation of peaks as compared to the control. Our results suggested that the strain Dipodascus australiensis M-2 possess excellent lignin degradation and color reduction capability and can be applied in waste treatment systems for pulp and paper mill effluent. In present work we are reporting first hand information regarding biodegradation of lignin by a potent strain of Dipodascus australiensis and statistical optimization of the bioprocess.
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- 2022
15. RESIDENTIAL QUALITY ASSESSMENT OF MULTI-STORY BUILDINGS THROUGH RESIDENCE EXPERIENCE: A CASE OF HYDERABAD, PAKISTAN
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Muhammad Ishtiaq Ali, Mir Aftab Hussain Talpur, Rija Shakir, Jawad Akbar Jamali, and Hassam Bin Waseem
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Geography ,Quality assessment ,Residence ,Socioeconomics - Abstract
In this modern era, multi-story housing is conceived as a sustainable and land-saving solution to increasing housing demand. In developing countries like Pakistan, unplanned and unsustainable multi-story housing development in major cities like Hyderabad. Housing quality is getting worse day by day, creating a negative impact on the physical and social well-being of inhabitants. The study identified the factors which are making a negative impact on multistory housing quality. The research is based on a thorough literature review, variables which helped categorize into safety and security, environment, life convenience, space utilization, utilities and services. To record the residents' opinion, the variables were inserted in a structured questionnaire with a 4-point Likert scale. Systematic sampling was applied to select a random sample of sixty buildings with an interval of 5. Using multiple regression analysis, four variables, including outdoor noise, fire protection, lack of elevator facility and drainage, were found to have a negative impact. The study is significant as the results can be considered as a guide for planning and development agencies to improve the multi-story housing quality by ensuring the inclusion of influential variables in the present and future development of multi-story buildings.
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- 2020
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16. High Potential of Methane Production from Coal by Fungi and Hydrogenotrophic Methanogens from Produced Water
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Muhammad Ishtiaq Ali, Hongguang Guo, Yixuan Zhang, Michael A. Urynowicz, Weiguo Liang, and Zaixing Huang
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animal structures ,Coalbed methane ,business.industry ,General Chemical Engineering ,Microorganism ,Coal mining ,Energy Engineering and Power Technology ,02 engineering and technology ,021001 nanoscience & nanotechnology ,Produced water ,Fuel Technology ,020401 chemical engineering ,Environmental chemistry ,embryonic structures ,Environmental science ,Coal ,0204 chemical engineering ,Methane production ,0210 nano-technology ,business ,High potential - Abstract
Studying in situ microorganisms is essential to understand the formation of biogenic coalbed methane (CBM). Only a few studies have investigated the fungi community inhabiting in coal seams althoug...
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- 2020
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17. Enhanced biogas production at mesophilic and thermophilic temperatures from a slaughterhouse waste with zeolite as ammonia adsorbent
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Hongguang Guo, Huan He, B. Fatima, Uzma Farooq, Muhammad Ishtiaq Ali, Fang-Jing Liu, Rabia Liaquat, Michael A. Urynowicz, Asif Jamal, and Zaixing Huang
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Environmental Engineering ,Chemistry ,Chemical oxygen demand ,010501 environmental sciences ,Pulp and paper industry ,01 natural sciences ,Methane ,Anaerobic digestion ,Ammonia ,chemistry.chemical_compound ,Biogas ,Fluidized bed ,Environmental Chemistry ,General Agricultural and Biological Sciences ,Zeolite ,0105 earth and related environmental sciences ,Mesophile - Abstract
Anaerobic digestion is a sustainable approach to deal with wastes with beneficial production of sustainable energy. In the anaerobic digestion processes, an increase in ammonia concentration hinders the efficacy of biogas production. The current study focused on minimizing the ammonia effect by the addition of zeolite as an adsorbent in a fluidized bed anaerobic reactor using poultry slaughterhouse waste as the substrate. The effects of zeolite dosage were investigated at mesophilic and thermophilic temperatures. Biomethane potential, volatile solids reduction, chemical oxygen demand, total volatile fatty acids concentration, ammonia–nitrogen levels, and pH were monitored. The initial biomethane potential test revealed that poultry slaughterhouse waste produces methane for up to 610 ml/g volatile solids (intestinal residues) and 200 ml/g volatile solids (feathers) at mesophilic conditions, 675 ml/g volatile solids (intestinal residues) and 276 ml/g volatile solids (feathers) at thermophilic conditions. The amendment with zeolite had significantly reduced chemical oxygen demand for up to 57%. Volatile solids reduction increased from 13 to 19%. Ammonia–nitrogen concentrations also decreased (15.5 mg/l) due to the application of zeolite. Enhanced biogas production of 700 ml/g volatile solids was observed after 25 days. It can be concluded that the addition of zeolite can significantly enhance biogas production by reducing the ammonia concentration in anaerobic digesters.
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- 2020
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18. Biodegradation of Concentrated Benzoic Acid Using White-Rot Fungus Hypocrea lixii AH
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Qian Zhang, Longfei Tang, Huan He, Zaixing Huang, Kai-yi Shi, Xiuxiang Tao, Michael A. Urynowicz, Muhammad Ishtiaq Ali, and Fen-Fen Hong
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inorganic chemicals ,biology ,Chemistry ,fungi ,02 engineering and technology ,010501 environmental sciences ,Biodegradation ,021001 nanoscience & nanotechnology ,biology.organism_classification ,01 natural sciences ,Pollution ,chemistry.chemical_compound ,Hypocrea ,Environmental Chemistry ,Degradation (geology) ,Food science ,White rot fungus ,0210 nano-technology ,Waste Management and Disposal ,0105 earth and related environmental sciences ,Benzoic acid - Abstract
Benzoic acid (BA) is potentially toxic to humans and recalcitrant in the environment. Although it may be degraded by fungi, potential degradation pathways has received limited study. In the present...
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- 2020
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19. Catalytic Efficiency of Acidithiobacillus ferrooxidans for Bioleaching Copper from Chalcocite Containing Sulfide Ore from Reko Diq Deposits
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Huda Ahmed Alghamdi, Rabia Liaquat, Bashir Ahmad, Uzma Farooq, Zahid Qureshi, Syed Kashif Haleem, Muhammad Ali Furqan, Asif Jamal, Muhammad Ishtiaq Ali, Isfahan Tauseef, and Inayat Ullah
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chemistry.chemical_classification ,Chalcocite ,Sulfide ,chemistry ,Bioleaching ,engineering ,Environmental Chemistry ,engineering.material ,Catalytic efficiency ,General Environmental Science ,Nuclear chemistry - Published
- 2020
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20. Fabrication of Biochar from Organic Wastes and its Effect on Wheat Growth and Soil Microflora
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Syed Kashif Haleem, Laiba Yaseen, Uzma Farooq, Sadia Aziz, Zahid Qureshi, Isfahan Tauseef, Asif Jamal, and Muhammad Ishtiaq Ali
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Fabrication ,Environmental chemistry ,Biochar ,Environmental Chemistry ,Environmental science ,Soil microbiology ,General Environmental Science - Published
- 2020
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21. Enhancing Biomethane Production From Lignite by an Anaerobic Polycyclic Aromatic Hydrocarbon Degrading Fungal Flora Enriched From Produced Water
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Kaiyi Gong, Yixuan Zhang, Hongguang Guo, Zaixing Huang, Michael Urynowicz, and Muhammad Ishtiaq Ali
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Microbiology (medical) ,Microbiology - Abstract
The coal-degrading ability of microorganisms is essential for the formation of biogenic coalbed methane. The ability to degrade the aromatic compound of coal is more important because it is perceived as the main refractory component for bioconversion. In this paper, a polycyclic aromatic hydrocarbon (PAH) degrading fungal community (PF) was enriched from produced water using phenanthrene as sole carbon source. The goal was to improve both the microbial structure of the methanogenic microflora and its coal-degrading ability. Two strategies were pursued. The first used coal pretreatment with PF (PP), followed by methane production by methanogenic microflora; the second used methane production directly from coal by mixed culture of PF and methanogenic microflora (PM). The results showed that methane productions of PP and PM increased by 29.40 and 39.52%, respectively. After 7 days of cultivation, the fungal community has been altered in PP and PM, especially for Penicillium the proportions of which were 67.37 and 89.81% higher than that in methanogenic microflora, respectively. Furthermore, volatile fatty acid accumulations increased by 64.21 and 58.15%, respectively. The 13C-NMR results showed that PF addition promoted the transformation of aromatic carbons in coal to carboxyl and carbonyl carbons, which contributed greatly to the production of methane together with oxygen-containing functional groups. These results suggest that methane production can be increased by indigenous PAH-degrading fungi by improving the fermentation of aromatics in coal and the generation of volatile fatty acids. This provided a feasible method for enhancing biomethane generation in the coal seam.
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- 2022
22. Contaminants from a Former Croatian Coal Sludge Dictate the Structure of Microbiota in the Estuarine (Raša Bay) Sediment and Soil
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Weiting Zhang, Qianyun Mo, Zaixing Huang, Muhammad Adnan Sabar, Gordana Medunić, Tatjana Ivošević, Huan He, Michael Urynowicz, Fang-Jing Liu, Hongguang Guo, Rizwan Haider, Muhammad Ishtiaq Ali, and Asif Jamal
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Microbiology (medical) ,Raša coal ,microbial diversity ,estuary ,PAHs ,hazardous trace elements ,natural attenuation ,Microbiology - Abstract
IntroductionCroatian superhigh-organic-sulfur Raša coal had been mined for nearly 400 years. The release of hazardous trace elements (HTEs) and toxic organic pollutants (TOPs) into the local environment by coal mining, preparation, and combustion activities has resulted in pollution.MethodsIn this study, the diversity and composition of microbial communities in estuarine sediment and soil samples as well as community function responses to the pollutants were investigated.ResultsThe results showed that PAH degradation does occur following 60 years of natural attenuation, the location is still heavily polluted by polycyclic aromatic hydrocarbons (PAHs) and HTEs. Microbial analyses have shown that high concentrations of PAHs have reduced the diversity and abundance of microbial communities. The pollution exerted an adverse, long-term impact on the microbial community structure and function in the brackish aquatic ecosystem. Microorganisms associated with the degradation of PAHs and sulfur-containing compounds have been enriched although the diversity and abundance of the microbial community have reduced. Fungi which are believed to be the main PAH degrader may play an important role initially, but the activity remains lower thereafter. It is the high concentrations of coal-derived PAHs, rather than HTEs, that have reduced the diversity and abundance of microbial communities and shaped the structure of the local microbiota.DiscussionThis study could provide a basis for the monitoring and restoration of ecosystems impacted by coal mining activities considering the expected decommission of a large number of coal plants on a global scale in the coming years due to growing global climate change concerns.
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- 2022
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23. Production of lipids biosynthesis from Tetradesmus nygaardii microalgae as a feedstock for biodiesel production
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Farhana Bibi, Muhammad Ishtiaq Ali, Mushtaq Ahmad, Awais Bokhari, Kuan Shiong Khoo, Muhammad Zafar, Saira Asif, Muhammad Mubashir, Ning Han, and Pau Loke Show
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Fuel Technology ,General Chemical Engineering ,Organic Chemistry ,Energy Engineering and Power Technology - Published
- 2022
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24. Methane Generation from Anthracite by Fungi and Methanogen Mixed Flora Enriched from Produced Water Associated with the Qinshui Basin in China
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Han Qing, Muhammad Ishtiaq Ali, Zaixing Huang, Hongguang Guo, Michael A. Urynowicz, and Jinlong Zhang
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biology ,General Chemical Engineering ,Anthracite ,General Chemistry ,Structural basin ,biology.organism_classification ,Methanogen ,Produced water ,Methane ,Article ,Chemistry ,chemistry.chemical_compound ,MIXED FLORA ,chemistry ,Environmental chemistry ,Environmental science ,China ,QD1-999 - Abstract
Biogenic coalbed methane (CBM) is generally believed to be formed by anaerobic bacteria and methanogens, while a few studies took fungi into account. Here, the microflora consisting of fungi and methanogens was enriched from the produced water associated with the Qinshui Basin using anthracite as the only carbon source. The maximum methane yield of 231 μmol/g coal was obtained after 22 days of cultivation under the optimum temperature of 35 °C, pH of 8, salinity of 0–2%, particle size of 0.075–0.150 mm, and the solid–liquid ratio of 1:30. It could remain active even after exposure to air for 24 h. Miseq results showed that the archaea were mainly composed of Methanocella, a hydrogenotrophic methanogen, followed by acetoclastic methanogen Methanosaeta and Methanosarcina, which could use various methanogenic substrates. The fungal communities mainly included Amorphotheca, Alternaria, Aspergillus, and Penicilium, which are all able to degrade complex organics such as aromatics and lignin. After cultivation, the crystal structure of anthracite became looser, as shown by XRD results, which might be due to the swelling effect caused by the destruction of the aromatic ring structure of coal under the function of fungi. The stretching vibration intensity of each functional group in coal decreased with cultivation, as revealed by FTIR. The GC-MS results showed that the concentration of alkanes and alcohols decreased significantly, which are the products of ring-opening of aromatics by fungi. These results suggested that fungi and methanogens in the coalbed also can syntrophically degrade coal effectively, especially for aromatics in coal.
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- 2021
25. Seed morphology using SEM techniques for identification of useful grasses in Dera Ghazi Khan, Pakistan
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Moona Nazish, Muhammad Zafar, Shafiq Ahmad, Fethi Ahmet Özdemir, Syed Nasar Shah, Mehmet Ali Kutlu, Shazia Sultana, Anwer Usma, Mushtaq Ahmad, Rabia Afza, Maryam Akram Butt, Neelam Rashid, and Muhammad Ishtiaq Ali
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Histology ,Morphology (linguistics) ,02 engineering and technology ,Biology ,Poaceae ,Plant Epidermis ,Caryopsis ,Surface pattern ,03 medical and health sciences ,0302 clinical medicine ,Reticulate ,Botany ,Pakistan ,Instrumentation ,food and beverages ,030206 dentistry ,021001 nanoscience & nanotechnology ,Family Poaceae ,Medical Laboratory Technology ,Epidermal Cells ,Plant Stomata ,Seeds ,Microscopy, Electron, Scanning ,Identification (biology) ,Anatomy ,0210 nano-technology - Abstract
In recent study, 15 taxa of family Poaceae from Dera Ghazi Khan were investigated for morphological characterization of seeds. Scanning electron microscopy (SEM) was utilized to study morphological features of grass seeds. Moreover, economic importance of studied taxa has been discussed in present research. Grass seeds or caryopsis were collected in their mature state from different areas of Dera Ghazi Khan. Collected caryopses were subjected to SEM to investigate surface sculpture, epidermal cell shape, and cell wall patterns. Caryopsis shape observed was elliptic to oval. Also, the morphology of plant is being investigated. Cell wall pattern observed was straight and wavy. Surface sculpturing varies from smooth to reticulate. Epidermal cell shape was either wavy or irregular. Hilum position is terminal in all species. Epidermal cell structure and cell wall pattern was not observable in some species. Major variations among studied taxa were observed in terms of seed surface pattern. Five types of caryopsis surface pattern were observed namely, striate, smooth, reticulate, papillate, and granulate. Investigated taxa illustrated variations in terms of studied morphological features. Utilization of SEM has been proved very helpful in exploring seed morphological features. These observed features can aid in delimitation and identification of various grass taxa. Recent research recommends the utilization of SEM for caryopsis morphology to solve the identification issue of problematic grass genera.
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- 2019
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26. Foliar epidermal anatomy of some selected wild edible fruits of Pakistan using light microscopy and scanning electron microscopy
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Muhammad Zafar, Zeynep Şahan, Omer Kilic, Ghulam Yaseen, Mushtaq Ahmad, Muhammad Ishtiaq Ali, Haleema Sadia, Lubna, Saeeda Naz, Abdul Ghani, Qamar Abbas, M. Z. Khan, Naveed Alam, and Shazia Sultana
- Subjects
Histology ,Scanning electron microscope ,Ficus ,02 engineering and technology ,Solanum nigrum ,Plant Epidermis ,Rosa moschata ,03 medical and health sciences ,0302 clinical medicine ,food ,Microscopy ,Pakistan ,Instrumentation ,biology ,Capparis spinosa ,food and beverages ,030206 dentistry ,Anatomy ,021001 nanoscience & nanotechnology ,biology.organism_classification ,Trichome ,food.food ,Plant Leaves ,Medical Laboratory Technology ,Epidermal Cells ,Fruit ,Plant Stomata ,Microscopy, Electron, Scanning ,Key (lock) ,Plants, Edible ,0210 nano-technology - Abstract
The present study is focused on the detailed foliar epidermal anatomy of some selected wild edible fruits (WEFs) from Pakistan using light microscopy (LM) and scanning electron microscopy (SEM). The studied species are Ficus racemosa L., Solanum nigrum L., Capparis spinosa L., Physalis divaricata D.Don, Rosa moschata Herrm. and Ribes orientale Desf. collected from various localities of Pakistan. The objective of the present study is to investigate qualitative and quantitative anatomical characters for the identification and differentiation of collected wild edible fruits. The characters studied are shape and size of epidermal cells, anticlinal wall pattern, trichome type and shape, average number of stomata, length and width of stomata and pore. The detailed microscopic investigation and variations in the characters recorded have a key role in the determination and authentication of wild edible fruits. This study possesses great potential for plant taxonomists to further evaluate the species at molecular and genetic levels.
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- 2019
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27. Degradation of low rank coal by Rhizopus oryzae isolated from a Pakistani coal mine and its enhanced releases of organic substances
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Zaixing Huang, Muhammad Ishtiaq Ali, Muhammad Farman, Noreen Ghulam Fatima, Michael A. Urynowicz, Aneela Younas Malik, Muhammad Adnan Sabar, and Asif Jamal
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biology ,business.industry ,Chemistry ,020209 energy ,General Chemical Engineering ,Organic Chemistry ,technology, industry, and agriculture ,Coal mining ,Rhizopus oryzae ,Energy Engineering and Power Technology ,Beneficiation ,Fraction (chemistry) ,02 engineering and technology ,Biodegradation ,Raw material ,biology.organism_classification ,complex mixtures ,Fuel Technology ,020401 chemical engineering ,Environmental chemistry ,0202 electrical engineering, electronic engineering, information engineering ,Degradation (geology) ,Coal ,0204 chemical engineering ,business - Abstract
Coal bio-transformation technologies can convert the neglected low rank coal reserves to alternative fuels and non-fuel organic chemicals. Fungal mediated coal degradation can serve as biological beneficiation for alternative substances. For enhanced biodegradation, fungi can be isolated from coal environments and optimized for coal degradation processes. In this study, an indigenous fungal isolate AD-1, identified as Rhizopus oryzae by fungal ITS sequences, was isolated from a low rank coal sample collected from Darra Adam Khel, Frontier Region Kohat, Pakistan. The AD-1 mediated coal degradation was optimized and showed a substantial release of organics at 1.5% glucose and 0.5% coal loading ratio within 11 days. The GC–MS analysis of the black liquid from coal solubilization showed a variety of organic compounds including aromatic acids, fatty acids, alkanes, amines, and amides. The depolymerized liquid extract was analyzed by High-Performance Size-Exclusion Chromatography (HPSEC) for determining the molecular mass distribution of the released organics. The release of the organic fragments had molecular mass distribution ranging between 1.5 kDa and 26.7 kDa. In addition, the released fraction and residual coal sample after solubilization were analyzed with Fourier transform infrared spectroscopy (FT-IR) to explore the structural changes aroused by the AD-1 fungal treatment. The AD-1 fungal strain has the abilities of decarboxylation and deamination, as well as breaking the side chains of the aromatic rings. This demonstrates that indigenous fungi from coal environments can enhance the biodegradation and shows a potential for producing chemical feedstock or alternative fuels from low rank coal.
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- 2019
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28. Taxonomic significance of caryopsis in subfamily Panicoideae (Poaceae) using scanning electron microscopy and light microscopy
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Muhammad Zafar, Nomana Kalsoom, Fethi Ahmet Özdemir, Anwer Usma, Omer Kilic, Mushtaq Ahmad, Farida Anjum, Shazia Sultana, Abdul Nazir, and Muhammad Ishtiaq Ali
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Histology ,Subfamily ,Surface Properties ,02 engineering and technology ,Poaceae ,Caryopsis ,03 medical and health sciences ,0302 clinical medicine ,Reticulate ,Cenchrus ciliaris ,Botany ,Microscopy ,Instrumentation ,biology ,Cenchrus (insect anatomy) ,030206 dentistry ,Classification ,021001 nanoscience & nanotechnology ,biology.organism_classification ,Medical Laboratory Technology ,Panicoideae ,Fruit ,Microscopy, Electron, Scanning ,Anatomy ,0210 nano-technology - Abstract
In recent study, 13 taxa of subfamily Panicoideae were investigated for morphological characterization of caryopsis. Light microscope (LM) and scanning electron microscope (SEM) were utilized to study macro- and micro-morphological caryopsis features respectively. Caryopsis size in studied taxa was recorded as 1.5-10 mm long and 1-4 mm wide. Caryopsis color was brown, green, yellow, and whitish-brown. Caryopsis shape studied was obovate, elliptic, linear oblate, and round shallowly obtriangular. Hilum position is grooved and depressed. Caryopsis compression type was lateral and dorsiventral. Major variations among studied taxa were observed in terms of caryopsis surface pattern and epicuticular projection types. Six types of caryopsis surface pattern were observed viz. scabrate, rugose, striate, reticulate, papillate, and scabridulous. Structures such as silica cells, bulges, spines, prickles, granules, and bicellular microhair were studied as epicuticular projections. Major variations were observed among Cenchrus pennisetiformis and Cenchrus ciliaris as both has entirely two different types of surface patterns and epicuticular projections. Anticlinal wall thickness and pattern as well as periclinal wall texture and level were investigated. The present research work emphasized on caryopsis characterization of subfamily Panicoideae and it is recommended to establish phylogeny within subfamily Panicoideae and with other subfamilies of Poaceae.
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- 2019
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29. POTENTIAL DISSEMINATION OF ANTIMICROBIAL RESISTANCE FROM SMALL SCALE POULTRY SLAUGHTERHOUSES IN PAKISTAN
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Asif Jamal, Jafar Ali, Hassan Waseem, and Muhammad Ishtiaq Ali
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Geography ,Antibiotic resistance ,Scale (ratio) ,business.industry ,business ,Agronomy and Crop Science ,Ecology, Evolution, Behavior and Systematics ,Biotechnology - Published
- 2019
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30. Effects of Seasonal Changes on the Patients Flux to Basic Health Units in Pakistan
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Zafeer Saqib, Asif Jamal, Jafir Hussain Shirazi, Abdul Rashid, and Muhammad Ishtiaq Ali
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Public Health, Environmental and Occupational Health ,Environmental science ,Public aspects of medicine ,RA1-1270 ,Atmospheric sciences ,Letter to the Editor ,Flux (metabolism) - Abstract
The article's abstract is not available.
- Published
- 2021
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31. Crude oil biodegradation potential of biosurfactant-producing Pseudomonas aeruginosa and Meyerozyma sp
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Mazhar Iqbal, Naeem Ali, Asif Jamal, Ramla Rehman, Muhammad Ishtiaq Ali, Malik Badshah, and Zaixing Huang
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Environmental Engineering ,Health, Toxicology and Mutagenesis ,Microorganism ,0211 other engineering and technologies ,02 engineering and technology ,010501 environmental sciences ,medicine.disease_cause ,01 natural sciences ,Micelle ,chemistry.chemical_compound ,Surface-Active Agents ,Bioremediation ,medicine ,Environmental Chemistry ,Food science ,Waste Management and Disposal ,0105 earth and related environmental sciences ,021110 strategic, defence & security studies ,Pseudomonas aeruginosa ,Biodegradation ,Contamination ,Pollution ,Biodegradation, Environmental ,Petroleum ,chemistry ,Saccharomycetales ,Degradation (geology) - Abstract
This study investigates the potential of crude oil degrading capabilities of biosurfactant-producing strains of Pseudomonas aeruginosa MF069166 and Meyerozyma sp. MF138126. P. aeruginosa produced mono-/di-rhamnolipids congeners whereas, Meyerozyma sp. produced acidic and lactonic forms of sophorolipids with crude oil. The values of critical micelle concentrations of rhamnolipids and sophorolipids were 40 mg/L and 50 mg/L with reductions in surface tension of water to 29 mN/m and 33 mN/m. Dynamic light scattering revealed that the average diameter of micellar aggregates of rhamnolipids ranged between 300 and 350 nm and the average size of sophorolipids micelles was 309 nm and 380 nm. Biosurfactants from P. aeruginosa and Meyerozyma sp. exhibited emulsification activities of 87% and 84% in crude oil. Cell surface hydrophobicity of both strains was higher in the presence of hydrophobic contaminants. The biosurfactants showed stability under varying pH, NaCl concentrations and temperatures. Gravimetric and GC-MS analyses demonstrated that P. aeruginosa degraded 91% of the petroleum hydrocarbons while Meyerozyma sp. showed 87% biodegradation efficiency. P. aeruginosa and Meyerozyma sp. have also been found to degrade halogen-containing compounds and showed excellent crude oil degradation efficiency. It is concluded that both strains have high potential of applications in the bioremediation of hydrocarbons-contaminated sites.
- Published
- 2021
32. Advancement and role of abiotic stresses in microalgae biorefinery with a focus on lipid production
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Farhana Bibi, Asif Jamal, Zaixing Huang, Michael Urynowicz, and Muhammad Ishtiaq Ali
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Fuel Technology ,General Chemical Engineering ,Organic Chemistry ,Energy Engineering and Power Technology - Published
- 2022
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33. Enhancement of biogenic methane production by co-degradation of coal and straw: microbial and organic analysis
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Kaixin Duan, Weiguo Liang, Yatong Cheng, Hongguang Guo, Muhammad Ishtiaq Ali, Michael A. Urynowicz, and Zaixing Huang
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biology ,Chemistry ,business.industry ,Methanogenesis ,Microorganism ,Straw ,biology.organism_classification ,Methanogen ,Methanosaeta ,Methanomethylovorans ,Methane ,chemistry.chemical_compound ,Environmental chemistry ,Coal ,business - Abstract
Co-degradation of coal and straw could produce significantly higher methane which was potential to increase biogenic CBM. In this study, the success of microflora and organic compounds during co-degradation was determined by MiSeq and GC-MS, and compared with cultivations with only coal (C) and with only straw (RS). The results showed that the methane production in co-degradation was 12 times higher than that in cultivation C. A shift of dominant methanogen was caused by the addition of straw from acetoclastic Methanosaeta in inoculum to methylotrophic Methanomethylovorans in 7 days, then hydrogenotrophic Methanobacterium. The bacteria and fungi with ability to degrade macromolecules in coal and metabolize VFAs were enriched which would facilitate methanogenesis. VFAs, especially butanoic acid, were dominant in intermediates of co-degradation which contributed to methane production as their content were negatively corelated with methane production. The different component of intermediates and microbial communities among co-degradation, cultivations C and RS suggested that the metabolic pathway in co-degradation was distinctive and the fracture of coal molecules was almost completed in the first 7 days of cultivation. Coal might also serve as the suitable microhabitat for microorganisms to avoid the threat from environment in addition to function as methanogenic substrates.
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- 2020
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34. Application and implication of scanning electron microscopy for evaluation of palyno-morphological features of Vitaceae from Pakistan
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Shabir Ahmad, Muhammad Ajmal Shah, Omer Kilic, Muhammad Zafar, Shazia Sultana, Fethi Ahmet Özdemir, Ghulam Mujtaba Shah, Erkan Yilmaz, Mushtaq Ahmad, Lubna, Muhammad Ishtiaq Ali, and Amir Muhammad Khan
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Histology ,Scanning electron microscope ,FAMILY VITACEAE ,030206 dentistry ,02 engineering and technology ,Biology ,021001 nanoscience & nanotechnology ,medicine.disease_cause ,Vitaceae ,biology.organism_classification ,03 medical and health sciences ,Medical Laboratory Technology ,0302 clinical medicine ,Genus ,Pollen ,Botany ,medicine ,Microscopy, Electron, Scanning ,Pakistan ,Anatomy ,0210 nano-technology ,Instrumentation ,Phylogeny - Abstract
The present study aimed to investigate the palyno-morphological features of species of family Vitaceae from Pakistan. A total of nine species, belonging to four genera were collected, pressed, identified, and then analyzed microscopically. Both quantitative and qualitative characters of the pollen grains were recorded including polar and equatorial diameter, P/E ratio, number of colpi and pores, exine thickness and shapes of the pollen in both polar and equatorial view, and exine sculpturing using Leica microscope fitted with camera Meiji Infinity 1 and then analyzed statistically using software IBM SPSS Statistics 20. The results of the present study demonstrated the variations in polar and equatorial diameter, exine thickness, P/E ratio, pollen shape, and exine sculpturing of the studied species and highlighted the significance of pollen morphology as an identification tool. The present study may contribute to better understand the classification at genus level, which will support the future phylogenetic characterization of the family.
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- 2020
35. Enzymatic decolorization of melanin by lignin peroxidase from Phanerochaete chrysosporium
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Beenish Sadaqat, Fang-Jing Liu, Uzma Farooq, Hongguang Guo, Asif Jamal, Zaixing Huang, Nazia Khatoon, Muhammad Ishtiaq Ali, Huan He, Qiurong Wang, Aneela Younas Malik, and Michael A. Urynowicz
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0301 basic medicine ,Time Factors ,Skin Lightening Preparations ,lcsh:Medicine ,Cosmetics ,Forests ,Phanerochaete ,01 natural sciences ,Article ,Melanin ,Fungal Proteins ,Applied microbiology ,03 medical and health sciences ,Extracellular ,Animals ,Humans ,lcsh:Science ,Cytotoxicity ,Incubation ,Benzyl Alcohols ,Soil Microbiology ,Chrysosporium ,chemistry.chemical_classification ,Melanins ,Multidisciplinary ,biology ,integumentary system ,Environmental microbiology ,010405 organic chemistry ,Chemistry ,lcsh:R ,Fungi ,Lignin peroxidase ,Hydrogen-Ion Concentration ,biology.organism_classification ,0104 chemical sciences ,030104 developmental biology ,Enzyme ,Biochemistry ,Peroxidases ,Proteolysis ,Microscopy, Electron, Scanning ,lcsh:Q ,sense organs ,Artemia - Abstract
Skin darkening results as a consequence of the accumulation of skin pigment melanin. To combat this, the amplitude of skin lightening agents are commercially available, most of which inhibit melanin synthesis. Decolorization of melanin is an alternative method of skin lightening. In this study, we show that lignin peroxidase (LiP), an extracellular enzyme purified from Phanerochaete chrysosporium NK-1 isolated from a forest soil can effectively degrade and decolorize melanin in vitro. Decolorization conditions including pH, temperature, incubation time, enzyme concentration, and mediator addition were investigated to optimize the reaction conditions. The results indicate that pH 3, 40 °C, 15 IU/ml, and 10 h incubation were the optimal conditions for the decolorization of the melanin. The use of the mediator, veratryl alcohol was also found effective to enhance the efficacy of the melanin decolonization, with up to 92% decolorization. The scanning electron microscopy results showed void spaces on the treated melanin granules as compared to the untreated sample, indicating the degradation of melanin. Changes in the fingerprint region of the melanin were observed. Between wavenumbers 1500–500 cm−1, for example, the presence of new peaks in the treated melanin at 1513, 1464, and 1139 cm−1 CH2, CH3 bend and C–O–C stretch represented structural changes. A new peak at 2144 cm−1 (alkynyl C≡C stretch) was also detected in the decolorized melanin. The cytotoxicity study has shown that the treated melanin and LiP have low cytotoxic effects; however, the mediator of veratryl alcohol could result in high mortality which suggests that its use should be meticulously tested in formulating health and skincare products. The findings of the study suggest that LiP produced by Phanerochaete chrysosporium has the potential to be used in the medical and cosmetic industries, particularly for the development of biobased cosmetic whitening agents.
- Published
- 2020
36. Enhanced bioremediation of diesel range hydrocarbons in soil using biochar made from organic wastes
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Fang-Jing Liu, Muhammad Ishtiaq Ali, Asif Jamal, Sadia Aziz, Zaixing Huang, Uzma Farooq, Michael A. Urynowicz, Huan He, and Hongguang Guo
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Bioaugmentation ,010504 meteorology & atmospheric sciences ,010501 environmental sciences ,Management, Monitoring, Policy and Law ,complex mixtures ,01 natural sciences ,Biostimulation ,Soil ,Bioremediation ,Biochar ,Animals ,Soil Pollutants ,Soil Microbiology ,0105 earth and related environmental sciences ,General Environmental Science ,General Medicine ,Biodegradation ,Pollution ,Hydrocarbons ,Biodegradation, Environmental ,Petroleum ,Environmental chemistry ,Charcoal ,Environmental science ,Cattle ,Female ,Cow dung ,Pyrolysis ,Sludge ,Environmental Monitoring - Abstract
Hydrocarbon contamination due to anthropogenic activities is a major environmental concern worldwide. The present study focuses on biochar prepared from fruit and vegetable waste and sewage sludge using a thermochemical approach and its application for the enhanced bioremediation (biostimulation and bioaugmentation) of diesel-polluted soil. The biochar was characterized using FTIR (Fourier-transform infrared spectroscopy), elemental analysis, surface area analysis, and pore analysis. Adsorption experiments showed that hydrocarbon degradation was attributed to biological processes rather than adsorption. The study found that various biochar amendments could significantly increase the rate of hydrocarbon biodegradation with removal efficiencies > 70%. Bioaugmentation using cow dung further improved the removal efficiency to 82%. Treatments showing the highest degree of removal efficiency indicated the presence of 27 different bacteria phyla with Proteobacteria and Actinobacteria as the most abundant phyla. The present study concludes that biochar amendments have great potential for enhancing the bioremediation of soils contaminated with diesel range hydrocarbons.
- Published
- 2020
37. Pediatric oropharyngeal candidiasis: A comprehensive study on risk factors and most prevalent species of Candida
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Jafir Hussain, Shirazi, Muhammad Ishtiaq, Ali, Zareen, Akhtar, Asif, Jamal, and Abdul, Rashid
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Male ,Antifungal Agents ,Risk Factors ,Candida albicans ,Candidiasis ,Prevalence ,Humans ,Infant ,Oropharynx ,Female ,Microbial Sensitivity Tests ,Fluconazole ,Pediatrics - Abstract
Oropharyngeal candidiasis is caused by opportunistic fungal pathogen candida. Decreased immune level whether in immunocompromised patients or in extreme age groups facilitate the progression of disease in vulnerable individuals. Current study focusses on evaluation of most prevalent species of Candida in pediatric patients and also assesses risk factors causing oropharyngeal candidiasis. Total of 1152 pediatric patients, previously diagnosed with oropharyngeal candidiasis from rural areas of District Bahawalpur were included in this study. Oral clinical swab samples were collected from these patients. From collected clinical swab samples, isolation, characterization and identification of Candida spp. was carried out to assess most prevalent species. Questionnaire was filled, with prior patients consent, for assessment of risk factors associated with oropharyngeal candidiasis. Candida albicans was the most prevalent species with 68.6% prevalence. While C. lusitaniae (a nonalbicans) was the least prevalent with 0.4% prevalence. Among other non-albicans, C. glabrata counted 12.9%, C. tropicalis 5.6%, C. krusei 8.5%, C. parapsilosis 3.1% and C. dublineiensis 1.1%. Associated risk factors included hygienic status of mother, patient's cleanliness and economic condition of the parents. Being disease of infancy, oropharyngeal candidiasis, is the oral infection with many episodes of relapse in pediatric patients and if not addressed properly may lead to severe invasive and noninvasive infections. There is dire need of integrated efforts to reduce the burden of infections in pediatrics specially and common population in general, by adopting various steps including more awareness programs, increasing economic status of the people, patient counselling and many other steps in rural areas.
- Published
- 2020
38. Contributors
- Author
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Taha Arooj, Asma Aftab, Muhammad Afzaal, Ali Ahmad, Fiaz Ahmad, Waqas Ahmad, Sarfraz Ahmed, Iftikhar Ahmed, Noor Ul Ain, Muhammad Sajid Hamid Akash, Qaisar Akram, Rizwan Ali, Jafar Ali, Muhammad Ishtiaq Ali, Zeshan Ali, Safdar Ali Mirza, Muhammad Sulman Ali Taseer, Muniza Almas, Arshia Amin, Mehroze Amin, Saadia Andleeb, Muzammil Anjum, Wajiha Anwar, Muhammad Arshad, Muhammad Ashfaq, Hajra Ashraf, Basit Ateeq, Muhammad Umer Farooq Awan, B. Balabanova, Isam Bashour, Tahira Batool, Sajida Begum, Syeda Aniqa Bukhari, Zoma Chaudhry, Tahir Ali Chohan, Surojeet Das, Erum Dilshad, Lara El-Gemayel, Shahid Hussain Farooqi, Fareeha Fiayyaz, Marium Fiaz, Sahrish Habib, Muhammad Zaffar Hashmi, Jouman Hassan, Munawar Hussain, Muhammad Ibrahim, Gilberto Igrejas, Ayesha Imran, Muhammad Javed Iqbal, Komal Jabeen, Deeba Javed, Ayesha Kabeer, Ghulam Mustafa Kamal, Saira Hafeez Kamran, Issmat I. Kassem, Srujana Kathi, Muhammad Khalid, Mohsin Khurshid, Sunil Kumar, Iram Liaqat, Mahnoor Majid, Bushra Manzoor, Iqra Mazhar, Bisma Meer, Sajid Mehmood, Arooj Mumtaz, Muhammad Naveed, Sania Niaz, Waqar Pervaiz, Patrícia Poeta, Hafsa Raja, Ayesha Ramzan, Hafsa Anwar Rana, Tazeen Rao, Umer Rashid, Kanwal Rehman, Muhammad Saif Ur Rehman, Luqman Riaz, Shakila Sabir, Rabia Safeer, Saima Saima, Hamza Saleem ur Rehman, Sumbal Sardar, Asfandyar Shahab, Sana Shifaqat, Anila Sikandar, Adriana Silva, Vanessa Silva, Aashna Srivastava, Ayesha Tahir, Habib Ullah, Francis Victor, Qianqian Wang, Hassan Waseem, Qingxiang Yang, Bushra Yaqub, Muhammad Younus, Wei Yuan, Rabeea Zafar, and Tehseen Zahra
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- 2020
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39. Recent Advances in Treatment Technologies for Antibiotics and Antimicrobial Resistance Genes
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Asif Jamal, Hassan Waseem, Muhammad Ishtiaq Ali, Jafar Ali, and Sana Jameel
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Resistant bacteria ,Antibiotic resistance ,Antimicrobial resistant bacteria ,medicine.drug_class ,business.industry ,Industrial scale ,Antibiotics ,medicine ,Waste treatment technologies ,Antimicrobial resistance genes ,Environmental systems ,Business ,Biotechnology - Abstract
Various water and waste treatment technologies are gaining attention for having great potential to curb antimicrobial resistance (AMR) dissemination in the environment. Most of the treatment technologies treat AMR by being able to degrade antimicrobial resistance genes (ARGs), lyse antimicrobial resistant bacteria (ARBs) and/ or oxidize the antibiotics whose presence in the environmental matrices contribute in the development and spread of AMR. A number of treatment technologies like aerobic and anaerobic digestion, membrane bioreactors, composting, nanoparticles and some disinfection-based mechanisms have already been evaluated, at industrial scale, for treating the antibiotics and associated ARBs and ARGs. These technologies, at present, are in use from the perspective of waste management and/or alternative energy but their place in formulating a broad and comprehensive strategy for controlling the spread of AMR in clinical and environmental systems has been realized. This chapter summarizes some of the important treatment technologies, illustrating their key features and efficacy in treating AMR. Studies that provide alternative views about the contributions of various treatment technologies in selecting resistant bacteria and genes are also highlighted in this chapter.
- Published
- 2020
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40. Global trends in ARGs measured by HT-qPCR platforms
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Jafar Ali, Hamza Saleem Ur Rehman, Hassan Waseem, Muhammad Javed Iqbal, and Muhammad Ishtiaq Ali
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Computer science ,business.industry ,Global problem ,Surveillance and monitoring ,Dna amplification ,business ,Biotechnology - Abstract
Antimicrobial resistance (AMR) is an emerging global problem. With the abuse and misuse of antibiotics' consumption, the problem of AMR is increasing. The need for surveillance and monitoring programs for tackling the ever-increasing problem of AMR dissemination has significantly been felt by the scientific community. High-throughput qPCR technology offers cost-effectiveness and high-throughput DNA amplification with efficient use of sample/DNA for monitoring AMR. The technology has already been applied in many studies for analyzing hundreds of antimicrobial-resistance genes (ARGs) in various types of samples. Apart from the clinical routes, AMR dissemination has also widely been reported in environmental samples such as rivers, lakes, wastewater treatment plants, animal manure, composts, soil, etc. This chapter summarizes some of the important studies utilizing HT-qPCR technologies for evaluating AMR in different parts of the world, elaborating the key results and essential features in understanding the complex dynamics of AMR in various environmental matrices.
- Published
- 2020
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41. A mini review on biotransformation of coal to methane by enhancement of chemical pretreatment
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Muhammad Adnan Sabar, Song Jin, Zaixing Huang, Michael A. Urynowicz, Dan Zhang, Yi Ren, Huan He, Rizwan Haider, Hongguang Guo, Fang-Jing Liu, Muhammad Ishtiaq Ali, Asif Jamal, and Paul H. Fallgren
- Subjects
Coalbed methane ,Waste management ,business.industry ,General Chemical Engineering ,Organic Chemistry ,Fossil fuel ,Coal mining ,Energy Engineering and Power Technology ,Context (language use) ,Methane ,chemistry.chemical_compound ,Fuel Technology ,chemistry ,Natural gas ,Environmental science ,Coal ,business ,Energy source - Abstract
Coalbed methane (CBM) has great environmental and economic values in addition to its energy content. For years natural gas has been considered a transitional cleaner energy source as it emits less carbon dioxide and other pollutants than other fossil fuels. Due to thebiogenic origin of CBM in many reservoirs, microbial enhancement may also be used to stimulate gas production in coal seams; however, the CBM output is relatively low when compared to other sources of natural gas. Studies have shown that chemical pretreatment of coal prior to gas production can significantly increase the bioconversion rate of coal. This review summarizes and discusses the mechanisms of coal biotransformation and biodegradation in the context of CBM production using chemical pretreatment methods, as well as the latest development in the field, in order to promote the further development of CBM resources.
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- 2022
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42. Microscopic investigations of some selected species of Papilionaceae through SEM and LM from Skardu valley, northern Pakistan
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Muhammad Zafar, Sofia Rashid, Saeed ur Rehman, Shabnum Shaheen, Mushtaq Ahmad, Shafiq Ahmad, Shomaila Ashfaq, Muhammad Ishtiaq Ali, Shazia Sultana, and Abida Bano
- Subjects
Histology ,Morphology (linguistics) ,02 engineering and technology ,Biology ,medicine.disease_cause ,Plant Epidermis ,03 medical and health sciences ,0302 clinical medicine ,Reticulate ,Pollen ,Botany ,medicine ,Pakistan ,Instrumentation ,Aperture (botany) ,food and beverages ,Sem analysis ,Fabaceae ,Trichomes ,030206 dentistry ,021001 nanoscience & nanotechnology ,Trichome ,Plant Leaves ,Medical Laboratory Technology ,Epidermal Cells ,Plant Stomata ,Anatomy ,0210 nano-technology - Abstract
Pollen morphology of 10 species and foliar epidermal anatomy of eight species of Papilionaceae from Skardu valley, northern Pakistan has been estimated for the first time. The present study was commenced with an aim to provide a detailed account of the pollen morphology by scanning electron microscopy and foliar epidermal anatomy by light microscopy. The pollen aperture was tricolporate with reticulate exine in the selected species. Stomata types are actinocytic, paracytic, and anomocytic. Irregular or polygonal with undulate or straight walls, epidermal cells were reported. A unique diversity was observed in the foliar trichomes that show the taxonomic significance of the discrimination of taxa. Non-glandular trichomes were observed in the selected species which are unicellular with thin, long and pointed apical cells. Pollen and foliar micro morphological characters proved to be helpful for the identification of taxa at a specific level.
- Published
- 2018
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43. Role of Servitization, Digitalization, and Innovation Performance in Manufacturing Enterprises
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Cong Sun, Lei Shen, and Muhammad Ishtiaq Ali
- Subjects
Environmental effects of industries and plants ,Renewable Energy, Sustainability and the Environment ,business.industry ,Geography, Planning and Development ,TJ807-830 ,ComputerApplications_COMPUTERSINOTHERSYSTEMS ,PSM-DID ,Manufacturing enterprises ,Management, Monitoring, Policy and Law ,TD194-195 ,digitalization ,Renewable energy sources ,enterprise innovation performance ,Environmental sciences ,Manufacturing ,Production (economics) ,GE1-350 ,Business ,Dynamic capabilities ,servitization ,dynamic capabilities ,Industrial organization ,Digitization - Abstract
The structure of the manufacturing industry has forced manufacturing companies to understand the importance of digitalization and servitization transformation, in terms of production and R&, D. In this study, we examine the relationship between servitization, digitization, and enterprise innovation performance through the lens of dynamic capabilities within enterprises. We also discuss the impact of the transformation servitization strategy on business innovation, and the mechanisms by which it impacts business innovation performance. The study’s findings indicate that servitization significantly contributes to innovation performance, and digitalization acts as a mediating mechanism between the proposed relationships. Thus, this article argues for the integration and growth of servitization and digitization.
- Published
- 2021
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44. Impact of Social Public Relations on Consumer Buying Behavior of Pakistan
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Muhammad Ishtiaq Ali and Nisar Ali
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Advertising ,Business - Published
- 2017
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45. Impact of Green Marketing on Consumer Buying Behavior
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Nighat Shamim and Muhammad Ishtiaq Ali
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Green marketing ,Business ,Marketing - Published
- 2017
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46. Parametric characterization and statistical optimization of Argemone ochroleuca (Mexican Poppy) methyl esters as a renewable source of energy
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Muhammad Ishtiaq Ali, Muhammad Zafar, Anam Fatima, Shazia Sultana, and Mushtaq Ahmad
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Biodiesel ,Renewable Energy, Sustainability and the Environment ,Chemistry ,business.industry ,020209 energy ,Energy Engineering and Power Technology ,02 engineering and technology ,Transesterification ,Catalysis ,Renewable energy ,chemistry.chemical_compound ,Fuel Technology ,Nuclear Energy and Engineering ,Poppy ,Yield (chemistry) ,Botany ,0202 electrical engineering, electronic engineering, information engineering ,Methanol ,business ,Parametric statistics ,Nuclear chemistry - Abstract
Argemone ochroleuca oil methyl esters (Biodiesel) optimization was carried out using statistical analysis by one-way ANOVA, determining the R2; finally, the results are evaluated by F-test. Reaction temperature and time were most influencing factors, and experimental model appears to be fit. One-step biodiesel synthesis was followed, using alkali (NaOH) catalyzed reaction. The maximum yield of biodiesel (91%) was obtained at 65°C, at reaction time 120 min, and 7:1 methanol to oil ratio.
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- 2017
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47. Characterizing Bacterial Consortia from an Anaerobic Digester Treating Organic Waste for Biogas Production
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Muhammad Ishtiaq Ali, Isfahan Tauseef, Rabia Liaquat, Muhammad Imran, Zahid Qureshi, Asif Jamal, and Uzma Farooq
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chemistry.chemical_classification ,Waste management ,020209 energy ,02 engineering and technology ,Biodegradation ,Isolation (microbiology) ,Anaerobic digestion ,Biogas ,Microbial population biology ,chemistry ,0202 electrical engineering, electronic engineering, information engineering ,Environmental Chemistry ,Environmental science ,Organic matter ,Anaerobic exercise ,General Environmental Science ,Biogas production - Published
- 2017
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48. Identification of novel nonedible oil seeds via scanning electron microscopy for biodiesel production
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Sumreen Dawood, Omer Kilic, Muhammad Zafar, Fethi Ahmet Özdemir, Mushtaq Ahmad, Khalid Ahmad, Moona Nazish, Maryam Akram Butt, Anwer Usma, Shazia Sultana, and Muhammad Ishtiaq Ali
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Biodiesel ,Histology ,Materials science ,Scanning electron microscope ,food and beverages ,Context (language use) ,030206 dentistry ,02 engineering and technology ,021001 nanoscience & nanotechnology ,Sustainable energy ,03 medical and health sciences ,Medical Laboratory Technology ,Horticulture ,0302 clinical medicine ,Biodiesel production ,Oil content ,Ultrastructure ,Potential source ,Anatomy ,0210 nano-technology ,Instrumentation - Abstract
Exploration of substitute energy feed-stocks is the much-debated topic in the scientific society due to increasing power crises and related ecological concerns. As a source of sustainable energy, biodiesel turns out to be the best alternative to petro fuels. In this context, nonedible oil-producing seeds might be a potential source for biodiesel production owing to their environment-friendly nature and cost-effectiveness. The current study, consequently, deals with the investigation and identification of micro-morphological characters between six novel nonedible oil-bearing seeds employing scanning electron microscopy as possible biodiesel feed-stocks. Light microscopic examinations show that seed size varies from 0.3 to 1.3 cm in width and 0.5 to 1.5 cm in. Additionally, a large difference in seed color ranges from dark brown, black, and various shades of light brown was also witnessed. The FFA content of the seeds ranges in 0.3-4.1 mg KOH/g, and the seed oil content fall in 30-65% (w/w) range. SEM-mediated seed ultrastructure investigations displays greater variation in seed size, shape, color, periclinal wall shape, and sculpturing and so on. All the seeds differ from rounded, ovoid, ovate, oblong, flattened, and elliptical shape. Greater variation in seed wall structure has been seen from angular, entire, irregular, straight, elongated, smooth, and polygonal. The periclinal wall arrangements show alteration from flat, depressed, elevated, smooth, pentagonal, bullate, and coarse seed margins. The results obtained from the current study suggest that scanning electron microscopy could be a beneficial tool in vitalizing the hidden micromorphological characters among various nonedible oil producing seeds, which eventually helps in exploration, correct identification, seed classification, and authentication in future.
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- 2019
49. Taxonomic significance of cypsela morphology in tribe Cichoreae (Asteraceae) using light microscopy and scanning electron microscopy
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Shazia Sultana, Sana Ayaz, Muhammad Zafar, Khalid Ahmad, Azize Demirpolat, Nomana Kalsoom, Ömer Kiliç, Muhammad Raza Ul Mustafa, Muhammad Ishtiaq Ali, Duygu N. Çobanoğlu, Rabia Afza, Mushtaq Ahmad, Jamil Raza, Abdul Ghani, and Mamoona Munir
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Microscopy ,Histology ,Morphology (linguistics) ,biology ,Scanning electron microscope ,030206 dentistry ,02 engineering and technology ,Asteraceae ,021001 nanoscience & nanotechnology ,biology.organism_classification ,Tribe (biology) ,Surface pattern ,03 medical and health sciences ,Medical Laboratory Technology ,0302 clinical medicine ,Botany ,Seeds ,Microscopy, Electron, Scanning ,Taxonomic rank ,Anatomy ,0210 nano-technology ,Instrumentation - Abstract
The current study deals with the detailed morphology investigation of eight Cypsela species belonging to tribe Cichoreae. The different Cypsela types were described, explained, compared, and their taxonomic significance is discussed in detail. Light microscopy (LM) and scanning electron microscopy (SEM) have been used to highlight quantitative and qualitative characters of underestudied species. Cypsela exhibit great diversity in macro and micromorphological features such as shape, color, length, width, anticlinal and periclinal wall patterns, surface patterns, epicuticular projections. Majority of Cypsela species were brownish in color and their size ranges from 2.16 to 3.98 mm in length and 1.16 to 0.82 mm in breadth. A great diversity in Cypsela shapes like oblanceolate to obovate, obovoid to cylindrical, obvate, narrowly lanceolate were observed. Most of the platelets having epicuticular projections were observed. The surface pattern on the cypsela surface varied from rugose papillate, verrucose papillate, and striated. On the basis of considerable variations observed, the present study can assist as useful constraints at various taxonomic levels. The aim of the present study is to provide a comprehensive description of the Cypsela morphology and to determine the extent to which these micro morphological data can be used as a taxonomic character to delineate various taxa belonging to the tribe Cichoreae.
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- 2019
50. Assessment of knowledge and attitude trends towards antimicrobial resistance (AMR) among the community members, pharmacists/pharmacy owners and physicians in district Sialkot, Pakistan
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Aansa Rukya Saleem, Hamza Saleem Ur Rehman, Muhammad Ishtiaq Ali, Mishal Choudri, Muhammad Subhan, Asif Jamal, Jafar Ali, Nooh Arif, Hassan Waseem, Aroosa Khan, and Fiza Sarwar
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Adult ,Male ,0301 basic medicine ,Microbiology (medical) ,Health Knowledge, Attitudes, Practice ,medicine.medical_specialty ,Adolescent ,Attitude of Health Personnel ,Resistance ,030106 microbiology ,Psychological intervention ,Pharmacist ,Resistance (psychoanalysis) ,Pharmacy ,Pharmacists ,lcsh:Infectious and parasitic diseases ,Antimicrobial Stewardship ,Young Adult ,03 medical and health sciences ,0302 clinical medicine ,Physicians ,Surveys and Questionnaires ,Drug Resistance, Bacterial ,Humans ,Antimicrobial stewardship ,Medicine ,lcsh:RC109-216 ,AMR ,Pakistan ,Pharmacology (medical) ,030212 general & internal medicine ,One health ,Response rate (survey) ,business.industry ,Research ,Public health ,Ownership ,Public Health, Environmental and Occupational Health ,Middle Aged ,Cross-Sectional Studies ,Infectious Diseases ,One Health ,Family medicine ,Female ,business - Abstract
Background Antimicrobial resistance (AMR) is an emerging threat to public health worldwide. A significant evidence has suggested that the knowledge and attitude trends among the community, pharmacists and physicians can play a critical role in managing the ever increasing threat of AMR. Methods A cross-sectional survey was performed using three specific self-administered questionnaires for community members, pharmacists/pharmacy owners and physicians on a randomly selected sample population of 473, 424 and 308 respectively. Bivariate and multivariate logistic regression and Pearson chi-square tests were performed during data analysis. Result A response rate of 81.2% (n = 385), 37.7% (n = 160) and 53.9% (n = 166) was achieved for general community, pharmacists/pharmacy owners and physicians respectively. More than half of the community participants (55.6%; n = 214) possess poor knowledge of AMR. Furthermore, knowledge and attitude of the community participants were also found to be significantly correlated (r2 = 0.02) with each other. In 90.6% (n = 145) of the pharmacies included in the survey, a qualified pharmacist was not present at the time of the operations. Only 36.9% physicians (n = 60) knew about the environmental route of dissemination of AMR. Majority of the physicians agreed that AMR is a global problem and also recognize the need for initiating AMR stewardship programs. Conclusion Our study will provide effective assessment and potential insights in designing tri-faceted interventions for rationalizing antibiotics consumption thus controlling the development and dissemination of AMR. Electronic supplementary material The online version of this article (10.1186/s13756-019-0517-3) contains supplementary material, which is available to authorized users.
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- 2019
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