423 results on '"Ramón Martínez-Máñez"'
Search Results
2. Ficin–Cyclodextrin-Based Docking Nanoarchitectonics of Self-Propelled Nanomotors for Bacterial Biofilm Eradication
- Author
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Miglė Žiemytė, Andrea Escudero, Paula Díez, María D. Ferrer, Jose R. Murguía, Vicente Martí-Centelles, Alex Mira, and Ramón Martínez-Máñez
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General Chemical Engineering ,Materials Chemistry ,General Chemistry - Published
- 2023
3. Colorimetric and fluorescent detection of synthetic cathinones in oral fluid with meso-aryl BODIPYs and Cu(<scp>ii</scp>)
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Jordi Hernández-Contreras, Margarita Parra, Salvador Gil, Ana M. Costero, Pau Arroyo, Félix Sancenón, Ramón Martínez-Máñez, José A. Sáez, and Pablo Gaviña
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QUIMICA ORGANICA ,General Chemical Engineering ,QUIMICA INORGANICA ,General Chemistry - Abstract
[EN] Synthetic cathinones are a class of new psychoactive substances whose consumption has increased a lot and is widespread throughout the world. Thus, there is currently a need for rapid and simple detection of these drugs. In particular, detection of synthetic cathinones in oral fluid in drivers can be of great importance in preventing traffic accidents. Herein, we report two probes, based on BODIPY derivatives combined with Cu(ii), which are able to detect these drugs both in water and in oral fluid, by changes in color and fluorescence. The determined limits of detection for ephedrone (as a model drug) are lower than the usual concentrations in saliva after intake of this type of drug. The sensing mechanism seems to be related to the cathinone induced reduction of Cu(ii) to Cu(i) with concomitants changes in the BODIPY structure., We thank the Spanish Government, MCIN/AEI/10.13039/501100011033 and "ERDF A way of making Europe" (fund RTL2018-100910-B-C42), the Spanish Ministry of Health, Consumer Affairs and Social Welfare (Project 2020I040 PNSD 2020) and Generalitat Valenciana (PROMETEO 2018/024) for financial support. SCSIE (Universitat de Valencia) is gratefully acknowledged for all the equipment employed. NMR was registered at the U26 facility of ICTS "NAMBIOSIS" at the Universitat of Valencia.
- Published
- 2022
4. β-Galactosidase-Activatable Nile Blue-Based NIR Senoprobe for the Real-Time Detection of Cellular Senescence
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Beatriz Lozano-Torres, Alba García-Fernández, Marcia Domínguez, Félix Sancenón, Juan F. Blandez, and Ramón Martínez-Máñez
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Analytical Chemistry - Abstract
Cellular senescence is a stable cell cycle arrest in response to stress or other damage stimuli to maintain tissue homeostasis. However, the accumulation of senescent cells can lead to the progression of various senescence-related disorders. In this paper, we describe the development of a β-galactosidase-activatable near-infrared (NIR) senoprobe
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- 2022
5. Targeted-lung delivery of dexamethasone using gated mesoporous silica nanoparticles. A new therapeutic approach for acute lung injury treatment
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María D. Marcos, Alba García-Fernández, Mar Orzáez, Pedro Amorós, Ramón Martínez-Máñez, Viviana Bisbal, Mónica Sancho, and Félix Sancenón
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Acute Lung Injury ,Pharmaceutical Science ,Inflammation ,Lung injury ,Pharmacology ,Dexamethasone ,Mice ,In vivo ,medicine ,Animals ,Humans ,Lung ,business.industry ,respiratory system ,Silicon Dioxide ,respiratory tract diseases ,medicine.anatomical_structure ,Targeted drug delivery ,Nanoparticles ,Tumor necrosis factor alpha ,medicine.symptom ,business ,Glucocorticoid ,medicine.drug - Abstract
Acute lung injury (ALI) is a critical inflammatory syndrome, characterized by increased diffuse inflammation and severe lung damage, which represents a clinical concern due to the high morbidity and mortality in critical patients. In last years, there has been a need to develop more effective treatments for ALI, and targeted drug delivery to inflamed lungs has become an attractive research field. Here, we present a nanodevice based on mesoporous silica nanoparticles loaded with dexamethasone (a glucocorticoid extensively used for ALI treatment) and capped with a peptide that targets the TNFR1 receptor expressed in pro-inflammatory macrophages (TNFR-Dex-MSNs) and avoids cargo leakage. TNFR-Dex-MSNs nanoparticles are preferentially internalized by pro-inflammatory macrophages, which overexpressed the TNFR1 receptor, with the subsequent cargo release upon the enzymatic hydrolysis of the capping peptide in lysosomes. Moreover, TNFR-Dex-MSNs are able to reduce the levels of TNF-α and IL-1β cytokines in activated pro-inflammatory M1 macrophages. The anti-inflammatory effect of TNFR-Dex-MSNs is also tested in an in vivo ALI mice model. The administered nanodevice (intravenously by tail vein injection) accumulated in the injured lungs and the controlled dexamethasone release reduces markedly the inflammatory response (TNF-α IL-6 and IL-1β levels). The attenuation in lung damage, after treatment with TNFR-Dex-MSNs, is also confirmed by histopathological studies. Besides, the targeted-lung dexamethasone delivery results in a decrease of dexamethasone derived side-effects, suggesting that targeted nanoparticles can be used for therapy in ALI and could help to overcome the clinical limitations of current treatments.
- Published
- 2021
6. Synthesis and fluorescence sensing of energetic materials using benzenesulfonic acid-doped polyaniline
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Veerabhadragouda B. Patil, Shruthy D. Pattathil, Ramón Martínez-Máñez, Satish A. Ture, Channabasaveshwar V. Yelamaggad, and Venkataraman Abbaraju
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Quenching (fluorescence) ,Materials science ,Fluorophore ,QUIMICA INORGANICA ,Condensed Matter Physics ,Photochemistry ,Electrochemistry ,Cocrystal ,Atomic and Molecular Physics, and Optics ,Electronic, Optical and Magnetic Materials ,chemistry.chemical_compound ,symbols.namesake ,Benzenesulfonic acid ,chemistry ,Polyaniline ,symbols ,Electrical and Electronic Engineering ,Cyclic voltammetry ,Raman spectroscopy - Abstract
[EN] The Fluorescence sensing technique for trace detection of High Energy Materials (HEMs) has gained more attention in recent times. In the present paper, the interaction between the fluorophore and HEMs is studied using spectroscopic and electrochemical techniques. The fluorophore polyaniline (PANI) was functionalised by doping it with benzenesulfonic acid (BSA) to increase the processability, and mobility of ¿-electrons along with decreased ¿-stacking. It is observed that upon doping the solubility of BSA-PANI is increased, facilitating a higher quenching by commercial explosives, i.e., RDX, CL-20, CL-20:RDX cocrystal. The interaction studies undertaken though fluorescence quenching, FTIR and Resonance Raman studies shows that the benzenoid unit, polaron and bipolaron nitrogen in BSA-PANI interact with nitro groups of HEMs and form a charge-transfer complex between HEMs and BSA-PANI undergoing predominantly a PET mechanism. LOD value is found to be least for Cocrystal (1.876¿×¿10¿5 M) when compared to other HEMs 3.191¿×¿10¿5 M (CL-20), 5.904¿×¿10¿5 M (RDX), 3.734¿×¿10¿5 M (PETN) indicating that cocrystal can be detected in trace level. The collaborative study between cyclic voltammetry and the observed results of fluorescence quenching, revealed that the emeraldine salt form of (BSA-PANI) is sensitive to HEMs., The authors acknowledge and thank Prof. G. U. Kulkarni, the former Director of Centre for Nano and Soft Matter (CeNS) for providing the facility to work in the centre. The authors, S A Ture and V B Patil, expresses their thanks to M/s Premier Explosive Limited for their Financial support (H/A: 4254), the corresponding author expresses his thanks to UGC, New Delhi for the BSR faculty fellowship [F.4-5(11)/2019(BSR)]. We also thank the Spanish Government [RTI2018-100910-B-C41 (MCUI/AEI/FEDER, UE)] and Generalitat Valenciana (PROMETEO2018/024) for their support.
- Published
- 2021
7. Cellular senescence modulates progenitor cell expansion during axolotl limb regeneration
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Qinghao Yu, Hannah E. Walters, Giovanni Pasquini, Sumeet Pal Singh, Daniel León-Periñán, Andreas Petzold, Preethi Kesavan, Cristina Subiran, Ines Garteizgogeascoa, Dunja Knapp, Anne Wagner, Andrea Bernardos, María Alfonso, Gayathri Nadar, Andreas Dahl, Volker Busskamp, Ramón Martínez-Máñez, and Maximina H. Yun
- Abstract
SUMMARYAxolotl limb regeneration is accompanied by the transient induction of cellular senescence within the blastema, the structure which nucleates regeneration. The precise role of this blastemal senescent cell (bSC) population, however, remains unknown. Here, through a combination of gain- and loss-of-function assays, we elucidate the functions and molecular features of cellular senescence in vivo. We demonstrate that cellular senescence plays a positive role during axolotl regeneration, by creating a pro-proliferative niche that supports progenitor cell expansion and blastema outgrowth. Mechanistically, these effects are mediated by the provision of Wnt ligands by bSCs. Further, we uncover a link between Wnt signalling and senescence induction, and propose that bSC-derived Wnt signals facilitate the proliferation of neighbouring cells in part by preventing their induction into senescence. This work defines the roles of cellular senescence in regeneration of complex structures.
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- 2022
8. An enzyme-controlled mesoporous nanomachine for triple-responsive delivery
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Beatriz Mayol, Victor Dato, Manuel Rodriguez, Elena Lucena, Anabel Villalonga, Paula Díez, Sandra Jimenez-Falcao, Félix Sancenón, Alfredo Sánchez, Diana Vilela, Paloma Martínez-Ruiz, Ramón Martínez-Máñez, and Reynaldo Villalonga
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beta-Cyclodextrins ,Biomedical Engineering ,General Chemistry ,General Medicine ,Hydrogen Peroxide ,Silicon Dioxide ,Glucose Oxidase ,Glucose ,Doxorubicin ,Delayed-Action Preparations ,para-Aminobenzoates ,Humans ,General Materials Science ,Porosity - Abstract
The construction of a novel enzyme-controlled nanomachine with multiple release mechanisms for on-command delivery is described. This nanodevice was assembled by modifying mesoporous silica nanoparticles with 2-(benzo[d]thiazol-2-yl)phenyl 4-aminobenzoate moieties, and further capped with beta-cyclodextrin-modified glucose oxidase neoglycoenzyme. The device released the encapsulated payload in the presence of H2O2 and acidic media. The use of glucose as an input chemical signal also triggered cargo release through the enzymatic production of gluconic acid and hydrogen peroxide, and the subsequent disruption of the gating mechanism at the mesoporous surface. The nanodevice was successfully employed for the enzyme-controlled release of doxorubicin in HeLa cancer cells.
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- 2022
9. Colorimetric and fluorescent detection of synthetic cathinones in oral fluid with
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Jordi, Hernández-Contreras, Margarita, Parra, Salvador, Gil, Ana M, Costero, Pau, Arroyo, Félix, Sancenón, Ramón, Martínez-Máñez, José A, Sáez, and Pablo, Gaviña
- Abstract
Synthetic cathinones are a class of new psychoactive substances whose consumption has increased a lot and is widespread throughout the world. Thus, there is currently a need for rapid and simple detection of these drugs. In particular, detection of synthetic cathinones in oral fluid in drivers can be of great importance in preventing traffic accidents. Herein, we report two probes, based on BODIPY derivatives combined with Cu(ii), which are able to detect these drugs both in water and in oral fluid, by changes in color and fluorescence. The determined limits of detection for ephedrone (as a model drug) are lower than the usual concentrations in saliva after intake of this type of drug. The sensing mechanism seems to be related to the cathinone induced reduction of Cu(ii) to Cu(i) with concomitants changes in the BODIPY structure.
- Published
- 2022
10. Development of New Targeted Nanotherapy Combined with Magneto-Fluorescent Nanoparticles against Colorectal Cancer
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Gonçalo A. Marcelo, David Montpeyó, Joana Galhano, Ramón Martínez-Máñez, José Luis Capelo-Martínez, Julia Lorenzo, Carlos Lodeiro, Elisabete Oliveira, LAQV@REQUIMTE, and DQ - Departamento de Química
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targeted anticancer therapy ,Organic Chemistry ,target nanocarriers ,oral formulations ,colorectal cancer ,target drug delivery ,General Medicine ,Catalysis ,Computer Science Applications ,Inorganic Chemistry ,SDG 3 - Good Health and Well-being ,Physical and Theoretical Chemistry ,Molecular Biology ,Spectroscopy - Abstract
Funding Information: This work received financial support from PT national funds (FCT/MCTES, Fundação para a Ciência e Tecnologia and Ministério da Ciência, Tecnologia e Ensino Superior) through the projects UIDB/50006/2020 and UIDP/50006/2020. G.M. acknowledges the financial support by the Associate Laboratory Research Unit for Green Chemistry—Clean Processes and Technologies—LAQV, which is financed by national funds from FCT/MEC (UID/QUI/50006/2013) and co-financed by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER—007265), as well as the Scientific Society PROTEOMASS (Portugal) for funding support (General Funding Grant). G.M. and J.G. thank FCT/MEC (Portugal) for their doctoral grant PD/BD/142865/2018 and 2022.09495.BD, respectively. E.O. thanks FCT (Fundação para a Ciência e Tecnologia) for funding through the Scientific Employment Stimulus-Individual Call (Ref. CEECIND/00648/2017). J.L. acknowledges the funding received from the Ministerio de Ciencia, Innovación y Universidades, Spain, grant PID2021-127983OB-C22 funded by MCIN/AEI/10.13039/501100011033. RMM acknowledges the funding received from project PID2021-126304OB-C41 funded by MCIN/AEI /10.13039/501100011033/ and by European Regional Development Fund—A way of doing Europe and Generalitat Valenciana (CIPROM/2021/007). Publisher Copyright: © 2023 by the authors. The need for non-invasive therapies capable of conserving drug efficiency and stability while having specific targetability against colorectal cancer (CRC), has made nanoparticles preferable vehicles and principal building blocks for the development of complex and multi-action anti-tumoral approaches. For that purpose, we herein report the production of a combinatory anti-tumoral nanotherapy using the production of a new targeting towards CRC lines. To do so, Magneto-fluorescent NANO3 nanoparticles were used as nanocarriers for a combination of the drugs doxorubicin (DOX) and ofloxacin (OFLO). NANO3 nanoparticles’ surface was modified with two different targeting agents, a newly synthesized (anti-CA IX acetazolamide derivative (AZM-SH)) and a commercially available (anti-epidermal growth factor receptor (EGFR), Cetuximab). The cytotoxicity revealed that only DOX-containing nanosystems showed significant and even competitive cytotoxicity when compared to that of free DOX. Interestingly, surface modification with AZM-SH promoted an increased cellular uptake in the HCT116 cell line, surpassing even those functionalized with Cetuximab. The results show that the new target has high potential to be used as a nanotherapy agent for CRC cells, surpassing commercial targets. As a proof-of-concept, an oral administration form of NANO3 systems was successfully combined with Eudragit® enteric coating and studied under extreme conditions. publishersversion published
- Published
- 2023
11. Tailored cancer therapy by magnetic nanoparticle hyperthermia: A virtual scenario simulation method
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Roberto Montes-Robles, Hazael Montanaro, Myles Capstick, Javier Ibáñez-Civera, Rafael Masot-Peris, Eduardo García-Breijo, Nicolás Laguarda-Miró, and Ramón Martínez-Máñez
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Cancer therapy ,QUIMICA INORGANICA ,Health Informatics ,Hyperthermia, Induced ,INGENIERIA QUIMICA ,Computer Science Applications ,TECNOLOGIA ELECTRONICA ,Planning ,Magnetics ,Neoplasms ,Magnetic nanoparticles ,03.- Garantizar una vida saludable y promover el bienestar para todos y todas en todas las edades ,Humans ,Hyperthermia ,Computer Simulation ,Magnetite Nanoparticles ,Software ,3D virtual model - Abstract
Background and objective: Hyperthermia is a cancer treatment aiming to induce cell death by directly warming cancerous tissues above 40 °C. This technique can be applied both individually and together with other cancer therapies. The main challenge for researchers and medics is to heat only tumoral cells avoiding global or localized heating of sane tissues. The objective in this study is to provide a realistic virtual scenario to develop an optimized multi-site injection plan for tailored magnetic nanoparticle-mediated hyperthermia applications. Methods: A three-dimensional model of a cat's back was tested in three different simulation scenarios, showing the impact of magnetic nanoparticles in each specific environment configuration. Results: As a result of this study. This simulation method can, minimising the affection to healthy tissue. Conclusions: This virtual method will help real and personalized therapy planning and tailor the dose and distribution of magnetic nanoparticles for an enhanced hyperthermia cancer treatment., Computer Methods and Programs in Biomedicine, 226, ISSN:0169-2607, ISSN:1872-7565
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- 2022
12. Ultrafast Directional Janus Pt–Mesoporous Silica Nanomotors for Smart Drug Delivery
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Paula Díez, Andrea Escudero, Antoni Llopis-Lorente, Ramón Martínez-Máñez, Reynaldo Villalonga, and Elena Lucena-Sánchez
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Materials science ,General Physics and Astronomy ,Nanoparticle ,Context (language use) ,Nanotechnology ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Article ,Catalysis ,ultrafast self-propulsion ,General Materials Science ,Janus nanomotors ,directional motion ,Platinum ,on-command controlled release ,General Engineering ,Nanocontainer ,Mesoporous silica ,Silicon Dioxide ,021001 nanoscience & nanotechnology ,Controlled release ,0104 chemical sciences ,Pharmaceutical Preparations ,Targeted drug delivery ,drug delivery ,Drug delivery ,Nanoparticles ,0210 nano-technology ,Biosensor - Abstract
Development of bioinspired nanomachines with an efficient propulsion and cargo-towing has attracted much attention in the last years due to their potential biosensing, diagnostics, and therapeutics applications. In this context, self-propelled synthetic nanomotors are promising carriers for intelligent and controlled release of therapeutic payloads. However, the implementation of this technology in real biomedical applications is still facing several challenges. Herein, we report the design, synthesis, and characterization of innovative multifunctional gated platinum-mesoporous silica nanomotors constituted of a propelling element (platinum nanodendrite face), a drug-loaded nanocontainer (mesoporous silica nanoparticle face), and a disulfide-containing oligo(ethylene glycol) chain (S-S-PEG) as a gating system. These Janus-type nanomotors present an ultrafast self-propelled motion due to the catalytic decomposition of low concentrations of hydrogen peroxide. Likewise, nanomotors exhibit a directional movement, which drives the engines toward biological targets, THP-1 cancer cells, as demonstrated using a microchip device that mimics penetration from capillary to postcapillary vessels. This fast and directional displacement facilitates the rapid cellular internalization and the on-demand specific release of a cytotoxic drug into the cytosol, due to the reduction of the disulfide bonds of the capping ensemble by intracellular glutathione levels. In the microchip device and in the absence of fuel, nanomotors are neither able to move directionally nor reach cancer cells and deliver their cargo, revealing that the fuel is required to get into inaccessible areas and to enhance nanoparticle internalization and drug release. Our proposed nanosystem shows many of the suitable characteristics for ideal biomedical destined nanomotors, such as rapid autonomous motion, versatility, and stimuli-responsive controlled drug release.
- Published
- 2021
13. Chromo-fluorogenic probes for β-galactosidase detection
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Beatriz Lozano-Torres, Ramón Martínez-Máñez, Félix Sancenón, and Juan F. Blandez
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In vitro and in vivo detection ,02 engineering and technology ,Cellular senescence ,01 natural sciences ,Biochemistry ,Analytical Chemistry ,Residue (chemistry) ,QUIMICA ORGANICA ,In vivo ,beta-Galactosidase detection ,Beta-Galactosidase detection ,Animals ,Humans ,Monosaccharide ,Cellular Senescence ,Enzyme Assays ,Fluorescent Dyes ,chemistry.chemical_classification ,Reporter gene ,Chemistry ,Hydrolysis ,QUIMICA INORGANICA ,010401 analytical chemistry ,Galactose ,Galactosides ,Glycosidic bond ,beta-Galactosidase ,021001 nanoscience & nanotechnology ,In vitro ,0104 chemical sciences ,Enzyme ,Molecular Probes ,Chromo-fluorogenic probes ,Colorimetry ,0210 nano-technology ,Molecular probe - Abstract
[EN] beta-Galactosidase (beta-Gal) is a widely used enzyme as a reporter gene in the field of molecular biology which hydrolyzes the beta-galactosides into monosaccharides. beta-Gal is an essential enzyme in humans and its deficiency or its overexpression results in several rare diseases. Cellular senescence is probably one of the most relevant physiological disorders that involve beta-Gal enzyme. In this review, we assess the progress made to date in the design of molecular-based probes for the detection of beta-Gal both in vitro and in vivo. Most of the reported molecular probes for the detection of beta-Gal consist of a galactopyranoside residue attached to a signalling unit through glycosidic bonds. The beta-Gal-induced hydrolysis of the glycosidic bonds released the signalling unit with remarkable changes in color and/or emission. Additional examples based on other approaches are also described. The wide applicability of these probes for the rapid and in situ detection of de-regulation beta-Gal-related diseases has boosted the research in this fertile field, R.M laboratory members received the financial support from the Spanish Government (project RTI2018-100910-B-C41) and the Generalitat Valenciana (project PROMETEO 2018/024). B.L-T. received support from the Spanish Ministry of Economy for their PhD grants (FPU15/02707). J. F.-B received fellowship (CD19/00038)
- Published
- 2021
14. A fluorogenic capped mesoporous aptasensor for gluten detection
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Elena Aznar, Luis Pla, Félix Sancenón, M. Carmen Martínez-Bisbal, Sara Santiago-Felipe, and Ramón Martínez-Máñez
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Glutens ,Aptamer ,Aptamers ,digestive system ,Biochemistry ,Gliadin ,Analytical Chemistry ,Global population ,QUIMICA ORGANICA ,Limit of Detection ,QUIMICA ANALITICA ,Humans ,Environmental Chemistry ,Effective treatment ,Electrodes ,Spectroscopy ,chemistry.chemical_classification ,Detection limit ,Chromatography ,Signalling process ,biology ,QUIMICA INORGANICA ,nutritional and metabolic diseases ,Aptasensor ,Molecular gates ,Gluten ,digestive system diseases ,Celiac Disease ,chemistry ,biology.protein ,Mesoporous material ,Mesoporous supports - Abstract
[EN] Celiac disease is a complex and autoimmune disorder caused by the ingestion of gluten affecting almost 1% of global population. Nowadays an effective treatment does not exist, and the only way to manage the disease is the removal of gluten from the diet. Owing the key role played by gluten, clear and regulated labelling of foodstuff and smart methods for gluten detection are needed to fight frauds on food industry and to avoid the involuntary ingestion of this protein by celiac patients. On that scope, the development of a novel detection system of gluten is here presented. The sensor consists of nanoporous anodic alumina films loaded with a fluorescent dye and capped with an aptamer that recognizes gliadin (gluten's soluble proteins). In the presence of gliadin, aptamer sequences displace from the surface of anodic alumina resulting in pore opening and dye delivery. The dispositive shows a limit of detection (LOD) of 100 mu g kg(-1) of gliadin, good selectivity and a detection time of approximately 60 min. Moreover, the sensor is validated in real food samples. This novel probe allows fast gluten detection through a simple signalling process with potential use for food control. (C) 2020 Elsevier B.V. All rights reserved., The authors want to thank the Spanish Government RTI2018-100910-B-C41 (MCUI/AEI/FEDER, UE) and the Generalitat Valenciana (project PROMETEO/2018/024) for support. L.P. thanks to Ministerio de Economia, Industria y Competitividad for his FPI grant. S.S.-F. thanks CIBER and ISCIII for her Sara Borrell contract. The authors also thank the Electron Microscopy Service at the UPV for support.
- Published
- 2021
15. A glutathione disulfide-sensitive Janus nanomachine controlled by an enzymatic AND logic gate for smart delivery
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Anabel Villalonga, Diana Vilela, Elena Lucena-Sánchez, Cristina de la Torre, Félix Sancenón, Beatriz Mayol, Paloma Martínez-Ruiz, Ramón Martínez-Máñez, Alfredo Sánchez, Paula Díez, and Reynaldo Villalonga
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Glutathione reductase ,Gating ,REDUCTASE ,HeLa ,chemistry.chemical_compound ,MESOPOROUS SILICA NANOPARTICLES ,Humans ,General Materials Science ,Nanodevice ,Glutathione Disulfide ,biology ,PEROXIDASE ,CARGO DELIVERY ,Glutathione ,Silicon Dioxide ,biology.organism_classification ,FTIR ,chemistry ,Doxorubicin ,Biophysics ,Nanoparticles ,Glutathione disulfide ,Gold ,Mesoporous material ,Porosity ,AND gate - Abstract
This work describes the assembly of a novel enzyme-controlled nanomachine operated through an AND Boolean logic gate for on-command delivery. The nanodevice was constructed on Au-mesoporous silica Janus nanoparticles capped with a thiol-sensitive gate-like molecular ensemble on the mesoporous face and functionalized with glutathione reductase on the gold face. This autonomous nanomachine employed NADPH and glutathione disulfide as input chemical signals, leading to the enzymatic production of reduced glutathione that causes the disruption of the gating mechanism on the mesoporous face and the consequent payload release as an output signal. The nanodevice was successfully used for the autonomous release of doxorubicin in HeLa cancer cells and RAW 264.7 macrophage cells.
- Published
- 2021
16. Aerogels as promising materials for antibacterial applications: a mini-review
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Huseyin Deveci, Elena Aznar, Ramón Martínez-Máñez, and Gulcihan Guzel Kaya
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Materials science ,Biocompatibility ,CELLULOSE AEROGELS ,Biomedical Engineering ,Nanotechnology ,CONTROLLED-RELEASE ,SILVER NANOPARTICLES ,engineering.material ,ENHANCED ANTIBACTERIAL ,Mini review ,Coating ,MESOPOROUS SILICA NANOPARTICLES ,General Materials Science ,DRUG-DELIVERY ,Porosity ,ANTIMICROBIAL RESISTANCE ,CARBON AEROGELS ,NANOCOMPOSITE AEROGELS ,Anti-Bacterial Agents ,Resistant bacteria ,IN-SITU SYNTHESIS ,engineering ,Surface modification ,Pharmaceutics ,Antibacterial activity ,Gels - Abstract
The increasing cases of bacterial infections originating from resistant bacteria are a serious problem globally and many approaches have been developed for different purposes to treat bacterial infections. Aerogels are a novel class of smart porous materials composed of three-dimensional networks. Recently, aerogels with the advantages of ultra-low density, high porosity, tunable particle and pore sizes, and biocompatibility have been regarded as promising carriers for the design of delivery systems. Recently, aerogels have also been provided with antibacterial activity through loading of antibacterial agents, incorporation of metal/metal oxides and via surface functionalization and coating with various functional groups. In this mini-review, the synthesis of aerogels from both conventional and low-cost precursors is reported and examples of aerogels displaying antibacterial properties are summarized. As a result, it is clear that the encouraging antibacterial performance of aerogels promotes their use in many antibacterial applications, especially in the food industry, pharmaceutics and medicine.
- Published
- 2021
17. Aptamer‐Capped Nanoporous Anodic Alumina for SARS‐CoV‐2 Spike Protein Detection
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Isabel Caballos, María Nieves Aranda, Alba López‐Palacios, Luis Pla, Sara Santiago‐Felipe, Andy Hernández‐Montoto, María Ángeles Tormo‐Mas, Javier Pemán, María Dolores Gómez‐Ruiz, Eva Calabuig, Beatriz Sánchez‐Sendra, Clara Francés‐Gómez, Ron Geller, Elena Aznar, and Ramón Martínez‐Máñez
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Mechanics of Materials ,General Materials Science ,Industrial and Manufacturing Engineering - Published
- 2023
18. Finite Element Models of Gold Nanoparticles and Their Suspensions for Photothermal Effect Calculation
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José Manuel Terrés-Haro, Javier Monreal-Trigo, Andy Hernández-Montoto, Francisco Javier Ibáñez-Civera, Rafael Masot-Peris, and Ramón Martínez-Máñez
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Finite Element Methods ,Bioengineering ,photothermal effect ,metal nanoparticles ,plasmonics - Abstract
(1) Background: The ability of metal nanoparticles to carry other molecules and their electromagnetic interactions can be used for localized drug release or to heat malignant tissue, as in the case of photothermal treatments. Plasmonics can be used to calculate their absorption and electric field enhancement, which can be further used to predict the outcome of photothermal experiments. In this study, we model the nanoparticle geometry in a Finite Element Model calculus environment to calculate the effects that occur as a response to placing it in an optical, electromagnetic field, and also a model of the experimental procedure to measure the temperature rise while irradiating a suspension of nanoparticles. (2) Methods: Finite Element Method numerical models using the COMSOL interface for geometry and mesh generation and iterative solving discretized Maxwell’s equations; (3) Results: Absorption and scattering cross-section spectrums were obtained for NanoRods and NanoStars, also varying their geometry as a parameter, along with electric field enhancement in their surroundings; temperature curves were calculated and measured as an outcome of the irradiation of different concentration suspensions; (4) Conclusions: The results obtained are comparable with the bibliography and experimental measurements.
- Published
- 2023
19. Strip-based lateral flow-type indicator displacement assay for γ-hydroxybutyric acid (GHB) detection in beverages
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Eva Garrido, Guillermo Hernández-Sigüenza, Estela Climent, M. Dolores Marcos, Knut Rurack, Pablo Gaviña, Margarita Parra, Félix Sancenón, Vicente Martí-Centelles, and Ramón Martínez-Máñez
- Subjects
Materials Chemistry ,Metals and Alloys ,Electrical and Electronic Engineering ,Condensed Matter Physics ,Instrumentation ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials - Published
- 2023
20. High-Capacity Mesoporous Silica Nanocarriers of siRNA for Applications in Retinal Delivery
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Amelia Ultimo, Mar Orzaez, Maria J. Santos-Martinez, Ramón Martínez-Máñez, María D. Marcos, Félix Sancenón, and Eduardo Ruiz-Hernández
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Inorganic Chemistry ,siRNA delivery ,large pore mesoporous silica nanoparticles ,VEGF silencing ,Organic Chemistry ,General Medicine ,Physical and Theoretical Chemistry ,age-related macular degeneration ,Molecular Biology ,Spectroscopy ,Catalysis ,Computer Science Applications - Abstract
The main cause of subretinal neovascularisation in wet age-related macular degeneration (AMD) is an abnormal expression in the retinal pigment epithelium (RPE) of the vascular endothelial growth factor (VEGF). Current approaches for the treatment of AMD present considerable issues that could be overcome by encapsulating anti-VEGF drugs in suitable nanocarriers, thus providing better penetration, higher retention times, and sustained release. In this work, the ability of large pore mesoporous silica nanoparticles (LP-MSNs) to transport and protect nucleic acid molecules is exploited to develop an innovative LP-MSN-based nanosystem for the topical administration of anti-VEGF siRNA molecules to RPE cells. siRNA is loaded into LP-MSN mesopores, while the external surface of the nanodevices is functionalised with polyethylenimine (PEI) chains that allow the controlled release of siRNA and promote endosomal escape to facilitate cytosolic delivery of the cargo. The successful results obtained for VEGF silencing in ARPE-19 RPE cells demonstrate that the designed nanodevice is suitable as an siRNA transporter.
- Published
- 2023
21. Correction to 'β-Galactosidase-Activatable Nile Blue-Based NIR Senoprobe for the Real-Time Detection of Cellular Senescence'
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Beatriz Lozano-Torres, Alba García-Fernández, Marcia Domínguez, Félix Sancenón, Juan F. Blandez, and Ramón Martínez-Máñez
- Subjects
Analytical Chemistry - Published
- 2023
22. Combination of palbociclib with navitoclax based-therapies enhances in vivo antitumoral activity in triple-negative breast cancer
- Author
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Alejandra Estepa-Fernández, Alba García-Fernández, Araceli Lérida-Viso, Juan F. Blandez, Irene Galiana, Félix Sancenon-Galarza, Mar Orzáez, and Ramón Martínez-Máñez
- Subjects
Pharmacology - Abstract
Triple-negative breast cancer (TNBC) is a very aggressive subtype of breast cancer with a poor prognosis and limited effective therapeutic options. Induction of senescence, arrest of cell proliferation, has been explored as an effective method to limit tumor progression in metastatic breast cancer. However, relapses occur in some patients, possibly as a result of the accumulation of senescent tumor cells in the body after treatment, which promote metastasis. In this study, we explored the combination of senescence induction and the subsequent removal of senescent cells (senolysis) as an alternative approach to improve outcomes in TNBC patients. We demonstrate that a combination treatment, using the senescence-inducer palbociclib and the senolytic agent navitoclax, delays tumor growth and reduces metastases in a mouse xenograft model of aggressive human TNBC (hTNBC). Furthermore, considering the off-target effects and toxicity derived from the use of navitoclax, we propose a strategy aimed at minimizing the associated side effects. We use a galacto-conjugated navitoclax (nav-Gal) as a senolytic prodrug that can preferentially be activated by β-galactosidase overexpressed in senescent cells. Concomitant treatment with palbociclib and nav-Gal in vivo results in the eradication of senescent hTNBC cells with consequent reduction of tumor growth, while reducing the cytotoxicity of navitoclax. Taken together, our results support the efficacy of combination therapy of senescence-induction with senolysis for hTNBC, as well as the development of a targeted approach as an effective and safer therapeutic opportunity.
- Published
- 2023
23. Multiplexed Detection of Analytes on Single Test Strips with Antibody‐Gated Indicator‐Releasing Mesoporous Nanoparticles
- Author
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Knut Rurack, Delia Gröninger, Mustafa Biyikal, Estela Climent, Michael G. Weller, and Ramón Martínez-Máñez
- Subjects
Analyte ,Materials science ,Explosive material ,Nanoparticle ,Nanotechnology ,STRIPS ,010402 general chemistry ,01 natural sciences ,Multiplexing ,Catalysis ,Analytical Chemistry ,law.invention ,law ,hybrid materials ,Research Articles ,Modularity (networks) ,multiplexing ,010405 organic chemistry ,explosives ,General Chemistry ,0104 chemical sciences ,signal amplification ,test-strip analysis ,Mesoporous material ,Biosensor ,Research Article - Abstract
Rapid testing methods for the use directly at a point of need are expected to unfold their true potential especially when offering adequate capabilities for the simultaneous measurement of multiple analytes of interest. Considering the unique modularity, high sensitivity, and selectivity of antibody‐gated indicator delivery (gAID) systems, a multiplexed assay for three small‐molecule explosives (TATP, TNT, PETN) was thus developed, allowing to detect the analytes simultaneously with a single test strip at lower ppb concentrations in the liquid phase in, The three small‐molecule explosives TATP, TNT and PETN can be detected simultaneously with a single test strip at lower ppb concentrations in the liquid phase in
- Published
- 2020
24. Multiplex‐Nachweis von Analyten auf einem einzelnen Teststreifen mit Antikörper‐gesteuerten und Indikator freisetzenden mesoporösen Nanopartikeln
- Author
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Knut Rurack, Delia Gröninger, Mustafa Biyikal, Ramón Martínez-Máñez, Michael G. Weller, and Estela Climent
- Subjects
010405 organic chemistry ,General Medicine ,010402 general chemistry ,01 natural sciences ,0104 chemical sciences - Abstract
Vor dem Hintergrund der einzigartigen Modularitat, hohen Empfindlichkeit und Selektivitat von Antikorper‐gesteuerten Indikatorfreisetzungssystemen (gAID‐Systemen) wurde hier ein Multiplex‐Assay fur drei organische Explosivstoffmolekule (TATP, TNT, PETN) entwickelt, der es erlaubt, die Analyten gleichzeitig in flussiger Phase mit einem einzelnen Teststreifen und einem Fluoreszenzlesegerat bzw. Smartphone als Detektor in Konzentrationen bis in den unteren ppb‐Bereich in
- Published
- 2020
25. Enhancement of photoactivity and cellular uptake of (Bu
- Author
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Cristina, de la Torre, Raquel, Gavara, Alba, García-Fernández, Maxim, Mikhaylov, Maxim N, Sokolov, Juan F, Miravet, Félix, Sancenón, Ramón, Martínez-Máñez, and Francisco, Galindo
- Subjects
Molybdenum ,Photosensitizing Agents ,Humans ,Antineoplastic Agents ,Silicon Dioxide ,Porosity - Abstract
The incorporation by ionic assembly of the hexanuclear molybdenum cluster (Bu
- Published
- 2022
26. Nanoprogrammed Cross-Kingdom Communication Between Living Microorganisms
- Author
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Beatriz de Luis, Ángela Morellá-Aucejo, Antoni Llopis-Lorente, Javier Martínez-Latorre, Félix Sancenón, Carmelo López, José Ramón Murguía, and Ramón Martínez-Máñez
- Subjects
cross-kingdom ,nanonetworks ,Mechanical Engineering ,chemical communication ,nanotranslator ,Nanotechnology ,General Materials Science ,Bioengineering ,cell communication ,Synthetic Biology ,General Chemistry ,Condensed Matter Physics ,microorganisms - Abstract
The engineering of chemical communication at the micro/nanoscale is a key emergent topic in micro/nanotechnology, synthetic biology, and related areas. However, the field is still in its infancy; previous advances, although scarce, have mainly focused on communication between abiotic micro/nanosystems or between microvesicles and living cells. Here, we have implemented a nanoprogrammed cross-kingdom communication involving two different microorganisms and tailor-made nanodevices acting as "nanotranslators". Information flows from the sender cells (bacteria) to the nanodevice and from the nanodevice to receiver cells (yeasts) in a hierarchical way, allowing communication between two microorganisms that otherwise would not interact.
- Published
- 2022
27. Purely Covalent Molecular Cages and Containers for Guest Encapsulation
- Author
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Giovanni Montà-González, Félix Sancenón, Ramón Martínez-Máñez, and Vicente Martí-Centelles
- Subjects
Binding Sites ,QUIMICA ORGANICA ,QUIMICA INORGANICA ,General Chemistry ,Catalysis - Abstract
[EN] Cage compounds offer unique binding pockets similar to enzyme-binding sites, which can be customized in terms of size, shape, and functional groups to point toward the cavity and many other parameters. Different synthetic strategies have been developed to create a toolkit of methods that allow preparing tailor-made organic cages for a number of distinct applications, such as gas separation, molecular recognition, molecular encapsulation, hosts for catalysis, etc. These examples show the versatility and high selectivity that can be achieved using cages, which is impossible by employing other molecular systems. This review explores the progress made in the field of fully organic molecular cages and containers by focusing on the properties of the cavity and their application to encapsulate guests., V.M.-C. acknowledges the financial support from project CIDEGENT/2020/031 funded by the Generalitat Valenciana and project PID2020-113256RA-I00 funded by MCIN/AEI/10.13039/501100011033. R.M.-M. and F.S. acknowledge the financial support from project PROMETEO 2018/024 from the Generalitat Valenciana and project RTI2018-100910-B-C41 funded by MCIN/AEI/10.13039/501100011033/and FEDER A way to make Europe. Funding for open access charge: CRUE-Universitat Politecnica de Valencia.
- Published
- 2022
28. Phosphorogenic dipyrrinato-iridium(III) complexes as photosensitizers for photodynamic therapy
- Author
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Alejandro Prieto-Castañeda, Edurne Avellanal-Zaballa, Maria J. Ortiz, Ramón Martínez-Máñez, Rebeca Sola-Llano, Jorge Bañuelos, Antonia R. Agarrabeitia, and Araceli Lérida-Viso
- Subjects
education.field_of_study ,Singlet oxygen ,Process Chemistry and Technology ,General Chemical Engineering ,medicine.medical_treatment ,Population ,Iridium(III) complexes ,chemistry.chemical_element ,Photodynamic therapy ,Photochemistry ,Photosensitizers ,Metal ,chemistry.chemical_compound ,Confocal imaging ,chemistry ,visual_art ,medicine ,visual_art.visual_art_medium ,Dipyrromethenes ,Iridium ,Triplet state ,Phosphorescence ,education - Abstract
We have designed and synthesized a family of Ir(III) metal complexes coordinated with two cyclometalated bis-fluorophenylpyridine ligands and an ancillary dipyrromethene which is functionalized with a mesityl group (Ir(dipy)-1), an α-chloroacetyl ester (Ir(dipy)-2) or a chain containing an ammonium cation (Ir(dipy)-3). The Ir(III) complexes feature a high triplet state population enabling red phosphorescence and efficient singlet oxygen generation. Ir(dipy)-2 and Ir(dipy)-3 are demonstrated to stain cells in both one-photon and two-photon confocal imaging. Moreover, Ir(dipy)-2 and Ir(dipy)-3 produce ROS in cells upon irradiation, inducing cell death by apoptosis. Colocalization studies in SK-Mel-103 cells show that Ir(dipy)-3 is partially accumulated in mitochondria and induces upon irradiation a disruption in their morphology. Overall our studies demonstrate that the prepared Ir(III) act as photosensitizers able to kill cells under irradiation, being suitable candidates for photodynamic therapy applications.
- Published
- 2022
29. Dualplex lateral flow assay for simultaneous scopolamine and 'cannibal drug' detection based on receptor-gated mesoporous nanoparticles
- Author
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Eva Garrido, Estela Climent, M. Dolores Marcos, Félix Sancenón, Knut Rurack, and Ramón Martínez-Máñez
- Subjects
QUIMICA ORGANICA ,Scopolamine ,QUIMICA INORGANICA ,Humans ,Nanoparticles ,General Materials Science ,Bethanechol ,Silicon Dioxide ,Nanostructures - Abstract
[EN] We report herein the design of a strip-based rapid test utilizing bio-inspired hybrid nanomaterials for the in situ and at site detection of the drug scopolamine (SCP) using a smartphone for readout, allowing SCP identification in diluted saliva down to 40 nM in less than 15 min. For this purpose, we prepared a nanosensor based on mesoporous silica nanoparticles loaded with a fluorescent reporter (rhodamine B) and functionalized with bethanechol, a potent agonist of recombinant human muscarinic acetylcholine receptor M-2 (M-2-AChR). M-2-AChR interaction with the anchored bethanechol derivative leads to capping of the pores. The sensing mechanism relies on binding of SCP to M-2-AChR resulting in pore opening and delivery of the entrapped rhodamine B reporter. Moreover, the material was incorporated into strips for lateral-flow assays coupled to smartphone readout, giving fast response time, good selectivity, and exceptional sensitivity. In an attempt to a mobile analytical test system for law enforcement services, we have also developed a dualplex lateral flow assay for SCP and 3,4-methylenedioxypyrovalerone (MDPV) also known as the so-called "cannibal drug"., This research was funded by the Ministerio de Ciencia, Innovacion y Universidades (Spanish Government), the Agencia Estatal de Investigacion (AEI), the European Union (projects RTI2018-100910-B-C41 and RTI2018-101599-B-C22-AR (MCIU/AEI/FEDER, EU), the Conselleria de Innovacion, Universidades, Ciencia y Sociedad Digital, Generalitat Valenciana (Project PROMETEO 2018/024) and the German Research Foundation (DFG; CL 761/1-19). E. G. is grateful to the Spanish MEC for her FPU grant (FPU16/02464).
- Published
- 2022
30. Lipofuscin labeling through biorthogonal strain-promoted azide-alkyne cycloaddition for the detection of senescent cells
- Author
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Beatriz Lozano‐Torres, Juan F. Blandez, Alba García‐Fernández, Félix Sancenón, and Ramón Martínez‐Máñez
- Subjects
QUIMICA INORGANICA ,detection ,Cell Biology ,Sudan Black B ,Cellular senescence ,Biochemistry ,Lipofuscin ,Detection ,QUIMICA ORGANICA ,alkyne-azide cycloaddition ,cellular senescence ,Alkyne-azide cycloaddition ,Molecular Biology ,lipofuscin - Abstract
[EN] A new method for senescent cell detection is described, which is based on lipofuscin labeling with a fluorescent reporter through a biorthogonal strain-promoted azide-alkyne cycloaddition. The sensing protocol involves a first step where the interaction of lipofuscin with a Sudan Black B derivative containing an azide moiety (SBB-N3 ) is carried out. In the final step, the azide moiety reacts with a fluorophore containing a cyclooctene ring (BODIPY). The efficacy of this two-step protocol is assessed in senescent melanoma SK-MEL-103 cells, senescent triple-negative breast cancer MDA-MB-231 cells and senescent WI-38 fibroblasts. In all cases, a clear fluorescence pattern was observed in senescent cells, compared to proliferative cells, only when the SBB-N3 -BODIPY probe was formed. Our results provide an alternative tool for the detection of senescent cells, based on an in situ bio-orthogonal reaction for lipofuscin labeling, RM laboratory members are grateful for financial support from the FEDER foundation of European Union (IDIFEDER/2021/044), the Spanish Government (projects RTI2018-100910-B-C41 and RTI2018-101599-B-C22) and the Generalitat Valenciana (project PROMETEO 2018/024). BL-T and AG-F acknowledge their current Margarita Salas postdoctoral fellowship from UPV-MIU and 'Next Generation EU' program. JF-B is grateful for his Sara Borrell postdoctoral fellowship (CD19/00038).
- Published
- 2022
31. Immunochemical Design of Antibody-Gated Indicator Delivery (gAID) Systems Based on Mesoporous Silica Nanoparticles
- Author
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Estela Climent, Michael G. Weller, Ramón Martínez-Máñez, and Knut Rurack
- Subjects
heterologous hapten ,QUIMICA INORGANICA ,Heterologous hapten ,Antibody-gated indicator delivery systems ,TATP ,Immunochemical response optimization ,antibody-gated indicator delivery systems ,signal amplification ,immunochemical response optimization ,Explosives detection ,test strip analysis ,General Materials Science ,explosives detection ,Test strip analysis ,Signal amplification - Abstract
[EN] In this work, the optimization of the immunochemical response of antibody-gated indicator delivery (gAID) systems prepared with mesoporous silica nanoparticles has been studied along various lines of system tailoring, targeting the peroxide-type explosive TATP as an exemplary analyte. The mechanism of detection of these gAID systems relies on a displacement of an antibody "cap" bound to hapten derivatives anchored to the surface of a porous hybrid material, allowing the indicator cargo stored in the mesopores to escape and massively amplify the analyte-related signal. Since our aim was to obtain gAID systems with the best possible response in terms of sensitivity, selectivity, and assay time, sera obtained from different immunization boosts were screened, the influence of auxiliary reagents was assessed, structural hapten modification (hapten heterology) was investigated, and various indicator dyes and host materials were tested. Considering that highly selective and sensitive immunological responses are best obtained with highaffinity antibodies which, however, could possess rather slow dissociation constants, leading to slow responses, the main challenge was to optimize the immunochemical recognition system for a rapid response while maintaining a high sensitivity and selectivity. The best performance was observed by grafting a slightly mismatching (heterologous) hapten to the surface of the nanoparticles in combination with high-affinity antibodies as "caps", yielding for the first time gAID nanomaterials for which the response time could be improved from hours to, This research was funded by the German Research Foundation (DFG; CL 761/1-19), the Spanish Government (project RTI2018-100910-B-C41 (MCUI/FEDER, EU)), and the Generalitat Valenciana (project PROMETEO 2018/024).
- Published
- 2022
32. Development of Geometry-Controlled All-Orthogonal BODIPY Trimers for Photodynamic Therapy and Phototheragnosis
- Author
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Alejandro Prieto-Castañeda, Fernando García-Garrido, Carolina Díaz-Norambuena, Blanca Escriche-Navarro, Alba García-Fernández, Jorge Bañuelos, Esther Rebollar, Inmaculada García-Moreno, Ramón Martínez-Máñez, Santiago de la Moya, Antonia R. Agarrabeitia, María J. Ortiz, Ministerio de Ciencia e Innovación (España), Instituto de Salud Carlos III, Eusko Jaurlaritza, Generalitat Valenciana, Comunidad de Madrid, and European Commission
- Subjects
Boron Compounds ,dimers ,Photosensitizing Agents ,Photochemotherapy ,Polymers ,QUIMICA INORGANICA ,Organic Chemistry ,rational design ,Química orgánica ,photosensitizers ,Physical and Theoretical Chemistry ,Biochemistry - Abstract
6 pags., 4 figs., We have established an easy synthetic protocol for selectively developing all-orthogonal BODIPY trimers with unprecedented geometries on the basis of selecting methyl oxidation versus electrophilic formylation of key dimeric precursors. Photophysical characterization together with biological assays unraveled the most suitable BODIPY-BODIPY geometrical arrangements within the trimer, forcing them to serve as molecular platforms for the development of new, advanced heavy-atom-free photosensitizers for photodynamic therapy and phototheragnosis., The authors acknowledge Spanish MICINN (PID2020-11455GB-C31, -C32, and -C33 and RTI2018-100910-BC41), Spanish Instituto de Salud Carlos III (IFI19/00026), Gobierno Vasco (IT1639-22), and Generalitat Valenciana (PROMETEO 2018/024) for financial support. A.P.-C. thanks Comunidad de Madrid for a research contract. C.D.-N. thanks MICINN for a FPI contract. B.E.-N. thanks the IFI19/00026 project, which is co-funded by the European Union, for a research contract.
- Published
- 2022
33. Electro-responsive films containing voltage responsive gated mesoporous silica nanoparticles grafted onto PEDOT-based conducting polymer
- Author
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Ramón Martínez-Máñez, Alba García-Fernández, María D. Marcos, Javier Monreal-Trigo, Miguel Alcañiz, Beatriz Lozano-Torres, Juan F. Blandez, Jorge E. Collazos-Castro, Juan Soto, and Félix Sancenón
- Subjects
Voltage-gated MSNs ,Materials science ,Biocompatibility ,Polymers ,Conducting polymers ,Pharmaceutical Science ,Nanoparticle ,02 engineering and technology ,Electro-responsive ,TECNOLOGIA ELECTRONICA ,03 medical and health sciences ,chemistry.chemical_compound ,QUIMICA ORGANICA ,PEDOT:PSS ,BIOQUIMICA Y BIOLOGIA MOLECULAR ,Rhodamine B ,Humans ,Controlled release ,Conducting polymers, Controlled release, Electro-responsive, PEDOT, Voltage-gated MSNs ,PEDOT ,030304 developmental biology ,Conductive polymer ,chemistry.chemical_classification ,0303 health sciences ,QUIMICA INORGANICA ,Polymer ,Mesoporous silica ,Bridged Bicyclo Compounds, Heterocyclic ,Silicon Dioxide ,021001 nanoscience & nanotechnology ,chemistry ,Chemical engineering ,Saturated calomel electrode ,Nanoparticles ,0210 nano-technology ,Porosity ,HeLa Cells - Abstract
[EN] The characteristics and electromechanical properties of conductive polymers together to their biocompatibility have boosted their application as a suitable tool in regenerative medicine and tissue engineering. However, conducting polymers as drug release materials are far from being ideal. A possibility to overcome this drawback is to combine conducting polymers with on-command delivery particles with inherent high-loading capacity. In this scenario, we report here the preparation of conduction polymers containing gated mesoporous silica nanoparticles (MSN) loaded with a cargo that is delivered on command by electro-chemical stimuli increasing the potential use of conducting polymers as controlled delivery systems. MSNs are loaded with Rhodamine B (Rh B), anchored to the conductive polymer poly(3,4-ethylenedioxythiophene) (PEDOT) doped with poly[(4-styrenesulfonic acid)-co-(maleic acid)], functionalized with a bipyridinium derivative and pores are capped with heparin (P3) by electrostatic interactions. P3 releases the entrapped cargo after the application of ¿640 mV voltage versus the saturated calomel electrode (SCE). Pore opening in the nanoparticles and dye delivery is ascribed to both (i) the reduction of the grafted bipyridinium derivative and (ii) the polarization of the conducting polymer electrode to negative potentials that induce detachment of positively charged heparin from the surface of the nanoparticles. Biocompatibility and cargo release studies were carried out in HeLa cells cultures., Alba Garcia-Fernandez, Beatriz Lozano-Torres contributed equally to this work. A. Garcia-Fernandez and B. Lozano-Torres are grateful to the "Ministerio de Economia y Competitividad" of the Spanish Government for her PhD fellowships. J. F. Blandez thanks the "Universitat Politecnica de Valencia" for his postdoctoral fellowship (PAID-10-17). The authors thank to the Spanish Government (Projects RTI2018-100910-B-C41 and RTI2018-101599-B-C22 (MCUI/AEI/FEDER, EU)) and the Generalitat Valencia (Project PROMETEO2018-024) for support.
- Published
- 2020
34. Preclinical antitumor efficacy of senescence-inducing chemotherapy combined with a nanoSenolytic
- Author
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Irene Galiana, Mar Orzáez, Mónica Sancho, María Alfonso, Viviana Bisbal, Beatriz Lozano-Torres, Ramón Martínez-Máñez, Manuel Serrano, and Andrea Bernardos
- Subjects
Senescence ,Mesoporous silica nanoparticles ,medicine.medical_treatment ,Senolysis ,Pharmaceutical Science ,Triple Negative Breast Neoplasms ,02 engineering and technology ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,QUIMICA ORGANICA ,Immune system ,Breast cancer ,Breast Cancer ,QUIMICA ANALITICA ,BIOQUIMICA Y BIOLOGIA MOLECULAR ,Animals ,Humans ,Medicine ,Senolytic ,Cellular Senescence ,Triple-negative breast cancer ,030304 developmental biology ,0303 health sciences ,Chemotherapy ,Navitoclax ,business.industry ,QUIMICA INORGANICA ,Cell Cycle Checkpoints ,021001 nanoscience & nanotechnology ,medicine.disease ,chemistry ,Cancer cell ,Cancer research ,0210 nano-technology ,business - Abstract
[EN] The induction of senescence produces a stable cell cycle arrest in cancer cells, thereby inhibiting tumor growth; however, the incomplete immune cell-mediated clearance of senescent cells may favor tumor relapse, limiting the long-term anti-tumorigenic effect of such drugs. A combination of senescence induction and the elimination of senescent cells may, therefore, represent an efficient means to inhibit tumor relapse. In this study, we explored the antitumor efficacy of a combinatory senogenic and targeted senolytic therapy in an immunocompetent orthotopic mouse model of the aggressive triple negative breast cancer subtype. Following palbociclib-induced senogenesis and senolysis by treatment with nano-encapsulated senolytic agent navitoclax, we observed inhibited tumor growth, reduced metastases, and a reduction in the systemic toxicity of navitoclax. We believe that this combination treatment approach may have relevance to other senescence-inducing chemotherapeutic drugs and additional tumor types. Significance: While the application of senescence inducers represents a successful treatment strategy in breast cancer patients, some patients still relapse, perhaps due to the subsequent accumulation of senescent cells in the body that can promote tumor recurrence. We now demonstrate that a combination treatment of a senescence inducer and a senolytic nanoparticle selectively eliminates senescent cells, delays tumor growth, and reduces metastases in a mouse model of aggressive breast cancer. Collectively, our results support targeted senolysis as a new therapeutic opportunity to improve outcomes in breast cancer patients., The M.O. laboratory members thank the financial support from the Spanish Government (project SAF2017-84689-R (MINECO/AEI/FEDER, EU)) and the Generalitat Valenciana (project PROMETEO/2019/065). The R.M. laboratory members thank the financial support from the Spanish Government (projects RTI2018-100910-B-C41 and RTI2018-101599-B-C22 (MCUI/FEDER, EU) and the Generalitat Valenciana (project PROMETEO 2018/024). Both I.G. and B.L-T. are grateful to the Generalitat Valenciana and the Spanish Ministry of Economy, respectively, for their Ph.D. grants. I.G. would like to thank I. Borreda and J. Forteza and the Instituto Valenciano de Patologia for their special collaboration and F. Sancenon for his appreciated help
- Published
- 2020
35. Mechanistic Insight into the Turn‐Off Sensing of Nitroaromatic Compounds Employing Functionalized Polyaniline
- Author
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Satish A. Ture, Venkata Narayanan Naranammalpuram Sundaram, Venkataraman Abbaraju, Lakshmidevi Venkatappa, Channabasaveshwar V. Yelamaggad, and Ramón Martínez-Máñez
- Subjects
Engineering ,Cyclic voltammetry ,business.industry ,QUIMICA INORGANICA ,Library science ,General Chemistry ,Picric acid ,Fluorescence quenching ,Doped polyaniline ,Turn off ,Raman spectroscopy ,business - Abstract
[EN] Conducting Polymers (CPs), in recent times have contributed significantly in the detection of High Energy Materials possessing nitro functional groups through fluorescence quenching studies. Camphor sulphuric acid doped polyaniline (C-PANI) is studied with a view to obtain easily processable PANI without pi-stacking for the detection of Picric acid(PA) and p-nitro toluene(pNT) in trace levels. The quenching constant and limit of detection were found to be 2.236x10(-6) M and 6.14x10(-7) M for PA and 1.9447x10(-7) M and 3.57x10(-7) M for pNT, respectively showing a very good sensitivity in detection. Resonance Raman spectroscopy (RRS) showed the involvement of the bipolaronic and benzenoid group in the charge transfer complex formed between the conducting polymer and the analyte. The photoluminescence studies indicated a predominant PET mechanism. The electrochemical studies employing cyclic voltammetry gave higher band gap on comparison with absorption studies. The positive and negative shift in oxidation and reduction potential of polymer-analyte was observed and collaborated with the fluorescence studies to understand the possible fluorescence quenching mechanism from electrochemical approach., The authors greatly acknowledge and thank Prof. G. U. Kulkarni, the Director of Centre for Nano and Soft Matter (CeNS) for providing the facility to work in the centre. Specifically, one of our author, Satish Ashok Ture, expresses his thanks to M/s Premier Explosive Limited for their Financial support (H/A: 4254). We also thank the Spanish Government (RTI2018-100910-B-C41 (MCUI/AEI/FEDER, UE)) and Generalitat Valenciana (PROMETEO2018/024) for their support. Part of this work is carried out under the Gulbarga University-Central University of Karnataka MoU program.
- Published
- 2020
36. Protection against chemical submission: naked-eye detection of γ-hydroxybutyric acid (GHB) in soft drinks and alcoholic beverages
- Author
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Silvia Rodríguez-Nuévalos, Pau Arroyo, Ana M. Costero, Félix Sancenón, Ramón Martínez-Máñez, Margarita Parra, and José A. Sáez
- Subjects
Chromatography ,Metals and Alloys ,General Chemistry ,Catalysis ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,chemistry.chemical_compound ,γ-Hydroxybutyric acid ,chemistry ,Materials Chemistry ,Ceramics and Composites ,sense organs ,Naked eye ,skin and connective tissue diseases ,Oxazole - Abstract
Two new oxazole derivatives, able to detect γ-hydroxybutyric acid (GHB) in soft drinks and alcoholic beverages, by color and fluorescence changes, are reported.
- Published
- 2020
37. Monofloral honey authentication by voltammetric electronic tongue: A comparison with
- Author
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Beatriz, Lozano-Torres, M, Carmen Martínez-Bisbal, Juan, Soto, Marisol, Juan Borrás, Ramón, Martínez-Máñez, and Isabel, Escriche
- Subjects
Magnetic Resonance Spectroscopy ,Pollen ,Food Contamination ,Honey ,Least-Squares Analysis ,Electronic Nose - Abstract
Proton-nuclear-magnetic-resonance-spectroscopy (
- Published
- 2021
38. Growth, Crystal Structure, Hirshfeld Surface Analysis, DFT Studies, Physicochemical Characterization And Cytotoxicity Assays of Novel Organic Triphosphate
- Author
-
Necmi Dege, Antoni Llopis Lorente, Ramón Martínez-Máñez, wajda sta, Yathreb Oueslati, Cristina de la Torre Paredes, and Sevgi Kansiz
- Subjects
Models, Molecular ,Chemistry ,Organic Chemistry ,Molecular Conformation ,Crystal structure ,Combinatorial chemistry ,Catalysis ,Computer Science Applications ,Characterization (materials science) ,Inorganic Chemistry ,Computational Theory and Mathematics ,Polyphosphates ,Physical and Theoretical Chemistry ,Crystallization ,Cytotoxicity - Abstract
A novel interesting organic-inorganic hybrid compound, named (1-phenylpiperazinium) trihydrogen triphosphate, with the formula (C10H15N2)2H3P3O10 has been obtained by low speed of evaporation at room temperature after using the ion exchange chemical procedure. To carry out a detailed crystallographic structure analysis, single-crystal X-ray diffraction has been reported. In the molecular arrangement, the different entities are held together through N-H…O, O-H…O and C-H…O hydrogen bonds, building up a three dimensional packing. Powder X-ray diffraction analysis is acquired to confirm the purity of the product. The nature and the proportion of intermolecular interactions were investigated by Hirshfeld surfaces analysis. In order to support the experimental results, a density functional theory (DFT) calculation were performed, using the Becke-3-Parameter-Lee-Yang-Parr (B3LYP) function with LANL2DZ basis set, and the data indicate the much agreement between the experimental and the theoretical results. Thus, the physicochemical properties were studied employing a variety of techniques (FT-IR, NMR, UV-Visible and photoluminescence). To get an insight of the possible employment of the present material in biology, cell viability assays were performed.
- Published
- 2021
39. Hollow mesoporous silica nanoparticles: Effective silica etching using tri-di- and mono-valent cations
- Author
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Leila Abdollahi, Mohammad Javad Dianat, Maria Dolores Marcos, Ramón Martínez-Máñez, and Sadegh Karimi
- Subjects
Biomaterials ,Cations ,Biomedical Engineering ,Nanoparticles ,Water ,Bioengineering ,Silicon Dioxide ,Porosity - Abstract
Among different hollow nanostructures, the preparation of hollow mesoporous silica nanoparticles (HMSNs) is still a hotspot research field due to their unique properties e.g., large pore sizes and volumes, high drug loading capacity, ease of surface modification, large surface area, and biodegradability. Herein, novel uniform HMSNs are prepared for the first time by a combination of heterogeneous oil-water biphase stratification and simple mono-, di-, and tri-valent etching reactions. The biphase stratification reaction allows self-assembly of reactants at the oil-water interface, while the subsequent step is designed for the efficient selective silica etching under mild conditions. We have studied the effect of cation's valence (NH
- Published
- 2021
40. Enhancement of photoactivity and cellular uptake of (Bu4N)2[Mo6I8(CH3COO)6] complex by loading on porous MCM-41 support. Photodynamic studies as an anticancer agent
- Author
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Cristina de la Torre, Raquel Gavara, Alba García-Fernández, Maxim Mikhaylov, Maxim N. Sokolov, Juan F. Miravet, Félix Sancenón, Ramón Martínez-Máñez, and Francisco Galindo
- Subjects
Biomaterials ,photodynamic therapy ,Biomedical Engineering ,Bioengineering ,HeLa cells ,melanoma cells ,mesoporous silica nanoparticles ,hexamolybdenum cluster ,singlet oxygen - Abstract
The incorporation by ionic assembly of the hexanuclear molybdenum cluster (Bu4N)2[Mo6I8(CH3CO2)6] (1) in amino-decorated mesoporous silica nanoparticles MCM-41, has yielded the new molybdenum-based hybrid photosensitizer 1@MCM-41. The new photoactive material presents a high porosity, due to the intrinsic high specific surface area of MCM-41 nanoparticles (989 m2 g-1) which is responsible for the good dispersion of the hexamolybdenum clusters on the nanoparticles surface, as observed by STEM analysis. The hybrid photosensitizer can generate efficiently singlet oxygen, which was demonstrated by using the benchmark photooxygenation reaction of 9,10-anthracenediyl-bis(methylene)dimalonic acid (ABDA) in water. The photodynamic therapy activity has been tested using LED light as an irradiation source (λirr ~ 400-700 nm; 15.6 mW/cm2). The results show a good activity of the hybrid photosensitizer against human cervical cancer (HeLa) cells, reducing up to 70 % their viability after 20 min of irradiation, whereas low cytotoxicity is detected in the darkness. The main finding of this research is that the incorporation of molybdenum complexes at porous MCM-41 supports enhances their photoactivity and improves cellular uptake, compared to free clusters. Ministerio de Ciencia, Innovación y Universidades of Spain (grant RTI2018-101675-B-I00) is acknowledged. F.G. thanks Universitat Jaume I (grant UJI-B2021-51) for the financial support. R.M.-M. laboratory members thank the financial support from the Spanish Government (project RTI2018-100910-B-C41) and the Generalitat Valenciana (project PROMETEO 2018/024). The research was supported by the Ministry of Science and Higher Education of the Russian Federation (M.N.S.). C.T. acknowledges the Generalitat Valenciana for her postdoctoral fellowship (APOSTD/2019/121). R.G. thanks Universitat Jaume I for a postdoctoral fellowship (POSTDOC-B/2018/09). We would like to thank Prof. Iván Mora-Seró (INAM-UJI) for the singlet oxygen phosphorescence measurements. The help of Jean Colombari in the final phase of this work is also recognized. SCIC-UJI is acknowledged for the technical support.
- Published
- 2022
41. Gated Organonanoclays for Large Biomolecules: Controlled Release Triggered by Surfactant Stimulus
- Author
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Elisa Poyatos-Racionero, Édgar Pérez-Esteve, Serena Medaglia, Elena Aznar, José M. Barat, Ramón Martínez-Máñez, Maria Dolores Marcos, and Andrea Bernardos
- Subjects
organonanoclay ,oleic acid ,surfactant ,General Chemical Engineering ,General Materials Science ,cyanocobalamin ,controlled release ,hematin - Abstract
The low toxicity and high adsorption capacities of clay minerals make them attractive for controlled delivery applications. However, the number of controlled-release studies in the literature using clay minerals is still scarce. In this work, three different clays from the smectite group (Kunipia F, montmorillonite; Sumecton SA, saponite; and Sumecton SWN, hectorite) were successfully loaded with rhodamine B dye and functionalized with oleic acid as a gatekeeper to produce organonanoclays for active and controlled payload-release. Moreover, hematin and cyanocobalamin have also been encapsulated in hectorite gated clay. These organonanoclays were able to confine the entrapped cargos in an aqueous environment, and effectively release them in the presence of surfactants (as bile salts). A controlled delivery of 49 ± 6 μg hematin/mg solid and 32.7 ± 1.5 μg cyanocobalamin/mg solid was reached. The cargo release profiles of all of the organonanoclays were adjusted to three different release-kinetic models, demonstrating the Korsmeyer–Peppas model with release dependence on (i) the organic–inorganic hybrid system, and (ii) the nature of loaded molecules and their interaction with the support. Furthermore, in vitro cell viability assays were carried out with Caco-2 cells, demonstrating that the organonanoclays are well tolerated by cells at particle concentrations of ca. 50 μg/mL.
- Published
- 2022
42. Monofloral honey authentication by voltammetric electronic tongue: A comparison with 1H NMR spectroscopy
- Author
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Beatriz Lozano-Torres, M. Carmen Martínez-Bisbal, Juan Soto, Marisol Juan Borrás, Ramón Martínez-Máñez, and Isabel Escriche
- Subjects
General Medicine ,Food Science ,Analytical Chemistry - Published
- 2022
43. A renal clearable fluorogenic probe for in vivo detection of cellular senescence
- Author
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Isabel Fariñas, Irene Galiana, Juan F. Blandez, Beatriz Lozano-Torres, Alba García-Fernández, Sara Rojas-Vázquez, Ramón Martínez-Máñez, Mar Orzáez, and Félix Sancenón
- Subjects
Text mining ,nervous system ,genetic structures ,In vivo ,Chemistry ,business.industry ,Cellular senescence ,business ,behavioral disciplines and activities ,psychological phenomena and processes ,Cell biology - Abstract
There is a growing need for non-invasive, cheap, and versatile diagnostic methods and the development of low-cost point-of-care assays constitutes a potential solution. Here, we describe the design of a renal clearable fluorogenic probe (Cy7Gal) which produces a fluorogenic signal readable in the urine. The Cy7Gal probe is applied to the detection of the burden of senescence in several in vivo models. The probe is composed of a dye (Cy7) conjugated with a galactose derivative. Upon administration of the probe in vivo, the up-regulated β-galactosidase enzyme in senescent cells cleaves the O-glycosidic bond in Cy7Gal, releasing the highly emissive Cy7 dye that is renally cleared and measured in unmodified urine by an IVIS imaging system or by a fluorimeter. A good correlation between the burden of senescence and emission in urine is observed. Cy7Gal probe is the first example for the fluorogenic in vivo detection of senescence in urine and may serve as a basis for the development of generalized fluorogenic diagnostic platforms for the easy diagnosis in the urine of different diseases as well as for monitoring therapeutic treatments without the use of expensive equipment or trained personnel.
- Published
- 2021
44. Vascular Senescence: A Potential Bridge Between Physiological Aging and Neurogenic Decline
- Author
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Sara Rojas-Vázquez, Laura Blasco-Chamarro, Irene López-Fabuel, Ramón Martínez-Máñez, and Isabel Fariñas
- Subjects
Parabiosis ,General Neuroscience ,Niche ,Neurogenesis ,neurogenic niche ,Neurosciences. Biological psychiatry. Neuropsychiatry ,Review ,Biology ,adult neural stem cell ,Neural stem cell ,Physiological Aging ,Bridge (graph theory) ,senescence-associated secretory phenotype ,Aging brain ,parabiosis ,Heterochrony ,Neuroscience ,endothelial cell senescence ,RC321-571 - Abstract
The adult mammalian brain contains distinct neurogenic niches harboring populations of neural stem cells (NSCs) with the capacity to sustain the generation of specific subtypes of neurons during the lifetime. However, their ability to produce new progeny declines with age. The microenvironment of these specialized niches provides multiple cellular and molecular signals that condition NSC behavior and potential. Among the different niche components, vasculature has gained increasing interest over the years due to its undeniable role in NSC regulation and its therapeutic potential for neurogenesis enhancement. NSCs are uniquely positioned to receive both locally secreted factors and adhesion-mediated signals derived from vascular elements. Furthermore, studies of parabiosis indicate that NSCs are also exposed to blood-borne factors, sensing and responding to the systemic circulation. Both structural and functional alterations occur in vasculature with age at the cellular level that can affect the proper extrinsic regulation of NSCs. Additionally, blood exchange experiments in heterochronic parabionts have revealed that age-associated changes in blood composition also contribute to adult neurogenesis impairment in the elderly. Although the mechanisms of vascular- or blood-derived signaling in aging are still not fully understood, a general feature of organismal aging is the accumulation of senescent cells, which act as sources of inflammatory and other detrimental signals that can negatively impact on neighboring cells. This review focuses on the interactions between vascular senescence, circulating pro-senescence factors and the decrease in NSC potential during aging. Understanding the mechanisms of NSC dynamics in the aging brain could lead to new therapeutic approaches, potentially include senolysis, to target age-dependent brain decline.
- Published
- 2021
45. An Interactive Model of Communication between Abiotic Nanodevices and Microorganisms
- Author
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Beatriz de Luis, José Gadea, José Ramón Murguía, Antoni Llopis-Lorente, Reynaldo Villalonga, Elena Aznar, Ramón Martínez-Máñez, Paola Rincón, and Félix Sancenón
- Subjects
Computer science ,Microorganism ,Green Fluorescent Proteins ,Microorganisms ,Nanotechnology ,mesoporous materials ,010402 general chemistry ,Models, Biological ,01 natural sciences ,Catalysis ,Silica nanoparticles ,nanonetworks ,QUIMICA ORGANICA ,abiotic nanodevices ,CIENCIA DE LOS MATERIALES E INGENIERIA METALURGICA ,QUIMICA ANALITICA ,BIOQUIMICA Y BIOLOGIA MOLECULAR ,microorganisms ,Nanodevice ,Abiotic component ,Abiotic nanodevices ,Microscopy, Confocal ,010405 organic chemistry ,molecular communication ,QUIMICA INORGANICA ,General Medicine ,General Chemistry ,Silicon Dioxide ,Mesoporous materials ,0104 chemical sciences ,Proof of concept ,Models of communication ,Saccharomycetales ,Nanoparticles ,Molecular communication ,abiotic nanodevices, mesoporous materials, microorganisms, molecular communication, nanonetworks ,Nanonetworks - Abstract
[EN] The construction of communication models at the micro¿/nanoscale involving abiotic nanodevices and living organisms has the potential to open a wide range of applications in biomedical and communication technologies. However, this area remains almost unexplored. Herein, we report, as a proof of concept, a stimuli¿responsive interactive paradigm of communication between yeasts (as a model microorganism) and enzyme¿controlled Janus Au¿mesoporous silica nanoparticles. In the presence of the stimulus, the information flows from the microorganism to the nanodevice, and then returns from the nanodevice to the microorganism as a feedback., B.dL. is grateful to the Spanish Government for her FPU PhD fellowship. The authors wish to thank the Spanish Government (projects RTI2018-100910-B-C41 (MCUI/AEI/FEDER, UE) and CTQ2017-87954-P), the Generalitat Valenciana (project PROMETEO2018/024), the Comunidad de Madrid (Project IND2017/BMD-7642) and CIBER-BBN (NANOCOM project) for support.
- Published
- 2019
46. Glucose-Responsive Enzyme-Controlled Mesoporous Nanomachine with a Layer-by-Layer Supramolecular Architecture
- Author
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Paloma Martínez-Ruiz, Félix Sancenón, Beatriz de Luis, Paula Díez, Alba García-Fernández, Reynaldo Villalonga, Antoni Llopis-Lorente, Ramón Martínez-Máñez, Sandra Jimenez-Falcao, and Alfredo Sánchez
- Subjects
biology ,Chemistry ,QUIMICA INORGANICA ,Biochemistry (medical) ,Layer by layer ,Biomedical Engineering ,Supramolecular chemistry ,Nanotechnology ,General Chemistry ,Mesoporous silica ,Cyclodextrin: Enzymes ,Biomaterials ,QUIMICA ORGANICA ,Colloidal gold ,QUIMICA ANALITICA ,BIOQUIMICA Y BIOLOGIA MOLECULAR ,biology.protein ,Gold nanoparticles ,Glucose oxidase ,Enzymes [Cyclodextrin] ,Mesoporous material ,Delivery ,Nanodevice ,Layer (electronics) - Abstract
[EN] Here we describe the construction of an integrated and pH-sensitive nanomachine with layer-by-layer supramolecular design and enzymatic control for on-command delivery. The nanodevice comprises a first layer of ß-cyclodextrin-coated gold nanoparticles as capping element of benzimidazole functionalized mesoporous silica nanoparticles, and a second control layer based on an adatamantane-modified glucose oxidase derivative. The nanomachine was selectively fuelled by glucose and successfully employed for the autonomous release of doxorubicin in HeLa cancer cells., Financial support from the Spanish Ministry of Economy and Competitiveness (projects CTQ2014-58989-P, CTQ2015-71936-REDT, CTQ2017-87954-P, and MAT2015-64139-C4-1-R) and generalitat Valenciana (project PROMETEO/2018/024) is gratefully acknowledged.
- Published
- 2019
47. The chemistry of senescence
- Author
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Ramón Martínez-Máñez, Manuel Serrano, Miguel Rovira, Beatriz Lozano-Torres, Alejandra Estepa-Fernández, Mar Orzáez, and Félix Sancenón
- Subjects
Senescence ,Cell cycle checkpoint ,In vivo ,Chemistry ,General Chemical Engineering ,fungi ,food and beverages ,Cellular senescence ,General Chemistry ,In vitro ,Cell biology - Abstract
Senescence is a state of permanent cell cycle arrest. Cellular senescence can promote tissue remodelling but can negatively affect regenerative capacities of tissues and contribute to inflammation and the progression of cancer and ageing-related diseases. This Review highlights the chemical characteristics of senescence and how we can target senescent cells with small molecules to induce senescence in hyperproliferative tissues. Alternatively, these small molecules can also be administered to inhibit senescence or eliminate senescent cells as the basis of a promising strategy to treat ageing-related diseases. We also describe advances in detecting senescence in in vitro and in vivo models, such that we can evaluate response to treatments intended to induce or eliminate senescent cells. Senescence is a state of permanent cell cycle arrest. This Review highlights the chemical characteristics of senescence and how we can use small molecules to target, detect or eliminate senescent cells, as well as to induce or inhibit senescence.
- Published
- 2019
48. A simple and easy-to-prepare imidazole-based probe for the selective chromo-fluorogenic recognition of biothiols and Cu(II) in aqueous environments
- Author
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M. Manuela M. Raposo, Sameh El Sayed, Hazem Essam Okda, Susana P. G. Costa, Rosa Cristina Moutinho Ferreira, Ismael Otri, Ramón Martínez-Máñez, Félix Sancenón, and Universidade do Minho
- Subjects
Engenharia e Tecnologia::Biotecnologia Industrial ,Biothiols ,General Chemical Engineering ,Displacement assay ,02 engineering and technology ,Engenharia Química [Engenharia e Tecnologia] ,010402 general chemistry ,01 natural sciences ,chemistry.chemical_compound ,Biotecnologia Industrial [Engenharia e Tecnologia] ,Imidazole ,Chromo-fluorogenic detection ,Cu(II) complex ,Detection limit ,chemistry.chemical_classification ,Emission quenching ,Science & Technology ,Aqueous solution ,Process Chemistry and Technology ,Glutathione ,021001 nanoscience & nanotechnology ,Fluorescence ,3. Good health ,0104 chemical sciences ,Amino acid ,chemistry ,Absorption (chemistry) ,0210 nano-technology ,Engenharia e Tecnologia::Engenharia Química ,Nuclear chemistry - Abstract
A new simple and easy-to-prepare imidazole-based probe 1 was synthesized and used to detect Cu(II) and biothiols (Cys, Hcy and GSH) in aqueous environments. Addition of increasing amounts of Cu(II) to water (pH 7.4)-acetonitrile 90:10 v/v solutions of probe 1 induced the apperance of a red-shifted absorption together with a marked colour change from colorless to deep blue. In addition, probe 1 was fluorescent and a marked emission quenching in the presence of Cu(II) was observed. The optical response is selective and other cations tested do not induce significant chromo-fluorogenic modulations. Limits of detection for Cu(II) of 0.7 and 3.2 μM using UV–visible and fluorescence data were determined. On the other hand, addition of Cys, Hcy and GSH to the deep-blue water (pH 7.4)-acetonitrile 90:10 v/v solutions of the 1-Cu(II) complex reulted in a marked bleaching together with the appearance of a highly emissive band centred at 475 nm. Other amino acids tested induced negligible response. The limits of detection for Cys, Hcy and GSH using 1-Cu(II) and emission data are 6.5, 5.0 and 10.2 μM, respectively. These optical changes were ascribed to a biothiol-induced demetallation process of the 1-Cu(II) complex that released the free probe. Besides, probe 1 is non-toxic and can be used for Cu(II) detection in HeLa cells., FCT - Generalitat Valenciana(PROMETEOII/2014/047); Spanish Government (projects MAT2015-64139-C4-1-R and AGL2015-70235-C2-2-R) and the Generalitat Valenciana (project PROMETEOII/2014/047). Thanks are also due to Fundação para a Ciência e a Tecnologia for financial support to the Portuguese NMR network (PTNMR, Bruker Avance III 400-Univ. Minho), FCT and FEDER (European Fund for Regional Development)-COMPETEQREN-EU for financial support to the research centre CQ/UM (Ref. UID/QUI/00686/2013 and UID/QUI/0686/2016), and a PhD grant to R. C. M. Ferreira (SFRH/BD/86408/2012), info:eu-repo/semantics/publishedVersion
- Published
- 2019
49. Simple Endotoxin Detection Using Polymyxin‐B‐Gated Nanoparticles
- Author
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Serena Medaglia, Ramón Martínez-Máñez, Félix Sancenón, Sameh El Sayed, Ismael Otri, and Elena Aznar
- Subjects
Mesoporous silica nanoparticles ,Nanoparticle ,Peptide ,010402 general chemistry ,01 natural sciences ,Catalysis ,chemistry.chemical_compound ,QUIMICA ORGANICA ,Endotoxin ,CIENCIA DE LOS MATERIALES E INGENIERIA METALURGICA ,medicine ,Rhodamine B ,Gated materials ,Nanodevice ,Polymyxin B ,Detection limit ,chemistry.chemical_classification ,010405 organic chemistry ,Chemistry ,QUIMICA INORGANICA ,Organic Chemistry ,Cationic polymerization ,General Chemistry ,Mesoporous silica ,Combinatorial chemistry ,0104 chemical sciences ,Fluorogenic detection ,medicine.drug - Abstract
[EN] A nanodevice based on mesoporous silica nanoparticles with rhodamine B in the pore framework, functionalized with carboxylates on the outer surface and capped with the cationic polymyxin B peptide, was used to selectively detect endotoxin in aqueous solutions with a limit of detection in the picomolar range., The authors thank the Spanish Government (MAT2015 64139-C4-1-R) and the Generalitat Valenciana (PROMETEO2018/024) for their support. I.O. thanks to Erasmus Mundus Programme, Action 2, Lot 1, Syria, for his predoctoral fellowship. S.S. is grateful to Spanish Ministerio de Economia y Competitividad for his Juan de la Cierva contract (FJCI-2015-27201).
- Published
- 2019
50. A Colorimetric Probe for the Selective Detection of Norepinephrine Based on a Double Molecular Recognition with Functionalized Gold Nanoparticles
- Author
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Pablo Gaviña, Ana M. Costero, Tania M. Godoy-Reyes, Ramón Martínez-Máñez, and Félix Sancenón
- Subjects
Catechol ,Chemistry ,QUIMICA INORGANICA ,Neurotransmitters ,Glutamic acid ,Combinatorial chemistry ,Benzaldehyde ,Aggregation ,chemistry.chemical_compound ,QUIMICA ORGANICA ,Molecular recognition ,Colloidal gold ,Bathochromic shift ,Gold nanoparticles ,Moiety ,General Materials Science ,Norepinephrine (NE) ,Colorimetric detection ,Boronic acid - Abstract
[EN] A simple colorimetric probe for the selective and sensitive detection of neurotransmitter norepinephrine (NE), an important biomarker in the detection of tumors such as pheochromocytoma and paraganglioma, is described. The sensing strategy is based on the use of spherical gold nanoparticles functionalized with benzaldehyde and boronic acid-terminated moieties. A double molecular recognition involving on one hand the aromatic aldehyde and the aminoalcohol group of NE, and on the other hand the boronic acid and the catechol moiety of the neurotransmitter, results in analyte triggered aggregation of the gold nanoparticles, leading to a bathochromic shift of the SPR band in the UV-vis spectrum of the probe and a clear change in the color of the solution from red to blue. Probe P1 shows a remarkable selectivity toward NE versus other catecholamine neurotransmitters (dopamine and epinephrine) and selected biomolecules (S-HIAA, L-Tyr, glucose, uric acid, Lys and glutamic acid). Moreover, a linear response to NE in the 0-1 mu M concentration range was observed and a limit of detection of 0.07 mu M in aqueous media was determined by UV-vis spectroscopy. The sensitivity of the probe toward NE in synthetic urine was also evaluated. In this medium, a limit of detection of 0.09 mu M was obtained which falls within the range of clinical interest, Financial support from the Spanish Government (Projects MAT2015-64139-C4-1-R and MAT2015-64139-C4-4-R) and the Generalitat Valencia (Projects PROMETEOII/2014/047 and AICO/2017/093) is gratefully acknowledged. T. Godoy-Reyes is grateful to the Generalitat Valenciana for her Santiago Grisolia fellowship.
- Published
- 2019
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