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Doxorubicin (DOX) is an effective chemotherapy drug, but its clinical use has adverse effects on male reproduction. However, there are few studies about the specific biological processes related to male reproduction or strategies for improving fertility protection. In this paper, we examined the effects of DOX on spermatogenesis and sperm function, and tested the possible protective role of melatonin (MLT) against DOX’s reproductive toxicity. DOX-treated mice showed signs of significantly impaired spermatogenesis, including vacuolated epithelial cells, decreased testis weights, and lowered sperm counts and motility. DOX also reduced germ cell proliferation (PCNA) and meiosis-related proteins (SYCP3), but this effect could be partially improved with MLT administration. HSPA2 expression was maintained, which indicated that although MLT did not improve sperm motility, it did have a significant protective effect on elongated sperm. IVF results showed that MLT could partially promote two-cell and blastocyte development that was restricted by DOX. MLT reversed DOX-driven changes in the testes, including the antioxidant indices of SOD1, CAT and PRDX6, and the apoptotic indices of BAX and Caspase3. These results suggest that MLT effectively prevents DOX-induced early reproductive toxicity, and increase our understanding of the molecular mechanisms underlying DOX’s effects on male reproduction and the protective mechanism of MLT.

Di-2-ethylhexyl phthalate (DEHP) harms mammalian testis development, yet the specific mechanism of its effect on sperm quality and function is unclear. In this study, male mice were administrated DEHP (200 mg/kg/day) via intragastric (i.g.) injection for 35 days. The sperm quality and function of DEHP-exposed mice were evaluated. DEHP exposure reduced the relative testis weight and serum testosterone levels. In addition, sperm count and motility parameters decreased significantly, which led to reduced sperm fertility characterized by reduced acrosome reaction rate, sperm-egg binding capacity and blastocyte formation. DEHP exposure decreased anti-oxidant indicators and the expressions of Cat, Sod1, Prdx6 and Sirt1 in the testis. DEHP-exposure also resulted in decreased proliferating cell nuclear antigen (PCNA) expression in mice testis, as well as the dose-dependent inhibition of the proliferation of GC-1 and GC-2 cells. These phenotypes may be related to increased cell apoptosis characterized by BAX/BCL2 and P53 up-regulation. DEHP exposure resulted in the down-regulation of SIRT1 and p-AKT in mice testis and decreased levels of GC-1and GC-2 cells. DEHP co-incubation with sperm in vitro resulted in decreased tyrosine phosphorylation and progressive motility, as well as p-AKT expression in capacitated sperm. Differential sperm proteomics identified 495 differentially expressed proteins, including 257 proteins down-regulated in the DEHP-exposure group. Bioinformatics analysis showed that proteins involved in sperm-egg interaction and fertilization processes were significantly down-regulated. Pathway analysis demonstrated that the adhesion pathway was enriched in down-regulated proteins, while the pathway associated with ribosomes was enriched in up-regulated proteins. Conclusively, DEHP exposure impaired male fertility by affecting sperm quality and function, and a pathway mediating the DEHP-induced decline in sperm quality and function was identified. The study provides additional information for understanding the molecular mechanisms of DEHP exposure and its effects on male reproduction.

Rural residential coal combustion (RRCC) has detrimental effects on air quality, climate, and human health. There are large uncertainties regarding emissions from RRCC owing to the lack of consideration of several key factors (e.g. combination modes of coal and stoves, combustion modes, and high temporal resolution). In this study, we provided a new estimation framework for RRCC emissions through a case study in the Beijing-Tianjin-Hebei (BTH) region, China. The emission estimations were improved according to four aspects, namely (1) coal-specific and stove-specific coal consumption was calculated based on face-to-face field interviews of 6700 valid volunteers/households covering 288 villages in 50 counties; (2) the influences of combustion modes (flaming and smoldering modes) on emissions were considered; (3) emissions of different fuel-stove combinations were estimated based on coal, stove, and combustion mode-specific RRCC consumption and localised emission factors; and (4) a method for emission estimation with high temporal resolution (1 h) was developed. The results indicated that RRCC emitted 413.6 kt SO2, 55.7 kt NOx, 5717.3 kt CO, 149.4 kt VOCs, 167.1 kt PM2.5, 18.2 kt EC, 32.5 kt OC, and 8.2 kt NH3 in 2016. The combination of bituminous coal and an advanced coal stove was the most significant contributor (20.7–71.8%) to various pollutant emissions. Coal combusted under the flaming mode contributed to most (81.9%) of the total coal consumption, and thus emitted the majority (50.8–99.8%) of pollutants, except for VOCs. Meanwhile, that under the smoldering mode only accounted for 18.1% of the total consumption, but contributed 49.2% and 74.7% of the CO and VOCs emissions, respectively. Two clear emission peaks occurred at approximately 7:00–9:00 and 18:00–20:00. The detailed coal consumption and emissions with high temporal and spatial resolution can provide sound data for further research on rural environmental issues and scientific support to pollution control strategies.

Rural residential coal combustion (RRCC) for household heating is a potentially important source of air pollution. However, little research has been done on the environmental impacts of RRCC. This study therefore investigated the impacts of RRCC on air pollution based on detailed household heating data obtained from intensive face-to-face interviews in Shandong province, China. The total contributions and specific contributions of coal, stoves, and coal–stove combinations to air pollution were simulated using the WRF-CAMx-PSAT model. The RRCC for heating had a considerable impact on air pollution, contributing 36.1, 9.1, and 16.1% of atmospheric SO2, NOx, and PM2.5 in winter, respectively. Different coal–stove combinations had different impacts on air pollution and mitigation efficiencies. The combination of bituminous coal and advanced coal stoves was the dominant contributor to air pollution, comprising 60.3–68.8% of the total RRCC contribution to different air pollutants. Sensitivity analyses indicated that bituminous coal burnt in a traditional stove had the highest mitigation efficiency (0.67 μg·m−3/10 kt) for atmospheric PM2.5 pollution, 4.1 times higher than that of anthracite briquette coal burnt in advanced coal stoves. Moreover, although RRCC is a near-surface emission source, it contributed considerably to regional pollution. Non-local RRCC emissions accounted for 21.8–74.6, 15.5–72.3, and 35.3–79.9% of the total contribution to SO2, NOx, and PM2.5 in different cities, respectively. The findings of this study improve understanding on the environmental impacts of rural emissions and can provide scientific support for the formulation of effective air pollution mitigation strategies.

One of the direction of rough sets theory is to extend the equivalence relation, using more general binary relation such as the partial order relation, tolerance relation or similarity relations instead of the strict equivalence relation. So the scope of application of rough sets theory could be extended. But in the use of these more general relation instead of equivalence relation, some good properties of the original Pawlak approximation space �� , �� may no longer be satisfied. In this paper, various properties of two pairs of lower and upper approximation operators and the relationship between them are acquired through analysis and proof. The two pairs of lower and upper approximation operator will have good properties when the binary relation R only satisfies reflexivity, and the two pairs of approximation operators are the same under the equivalence relation. Theoretical analysis shows that, in the process of extending classical rough set theory to generalized rough set theory, reflexivity is a minimum conditions to be satisfied, under this condition, the lower and upper approximation operators meeting the corresponding properties can be chosen to adapt to the requirement of practical application.

Gives a new control method, which is used in the AGC systems of cold rolling mill. TO the shortage of control method which is used now, analyses and synthesize dynamic mathematical model of AGC, presents a new method: method. Simulations of experiment indicate that the control method has good system performance and robust stability performance.