1. Identification of microRNA-target genes in mice hippocampus at 1 week after pilocarpine-induced status epilepticus
- Author
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Jing Tan, Xiao-Lin Wu, Xin-li Xiao, Jin-Song Zhou, Kai-Wei Si, Hua Tong, Yan-Bing Ma, Peng-Bo-Yang, Jian-Xin Liu, and Le-Fan Liu
- Subjects
0301 basic medicine ,Biophysics ,Hippocampus ,Status epilepticus ,Hippocampal formation ,Biology ,Biochemistry ,Epileptogenesis ,03 medical and health sciences ,Mice ,0302 clinical medicine ,Status Epilepticus ,Thyrotropin-releasing hormone receptor ,Gene expression ,microRNA ,medicine ,Animals ,RNA, Messenger ,Molecular Biology ,Gene ,Gene Expression Profiling ,Pilocarpine ,Reproducibility of Results ,Cell Biology ,MicroRNAs ,030104 developmental biology ,Gene Ontology ,Gene Expression Regulation ,030220 oncology & carcinogenesis ,Cancer research ,medicine.symptom - Abstract
MicroRNAs (miRNA) are believed to play a crucial role in the cause and treatment of temporal lobe epilepsy (TLE) by controlling gene expression in different stages of the disease. To investigate role of miRNA in the latent stage following status epilepticus, we first compared microRNA expression profiles in mice hippocampus at 1 week after pilocarpine-induced status epilepticus (SE) vs. controls in hippocampal tissues using Exiqon miRCURY LNA™ miRNAs Array. Then, the target genes of altered miRNAs were predicted using both TargetScan 7.1 and miRDB V5, and were further selected by intersecting with another independent mRNA expression profile dataset from the samples at the same time point. We found out 14 common genes as down miRNA target (up-mRNA) and 4 common genes as up miRNA target (down mRNA) in SE mice. miR-669m-3p-TRHR (thyrotropin releasing hormone receptor), miR-669m-3p-B3galt2 (β-1,3-Galactosyltransferase 2), miR-105-PDPN (Podoplanin) and miR-883b-3p-CLEC-2 (C-type-lectin-like-2) were found to be potential molecular mechanisms to modulate the calcium signaling pathway, glycosylation pathways and chemokine mediated inflammatory processes in mice hippocampus at 1 week after pilocarpine-induced SE, respectively. Our results offered potential novel insights into the cellular events in the mice hippocampus mediated by miRNASs-target genes that shape SE-evoked epileptogenesis.
- Published
- 2020