1. New candidate tumor-suppressor gene KLF6 and its splice variant KLF6 SV2 counterbalancing expression in primary hepatocarcinoma
- Author
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Tian De'an, Zhou Zhenzhen, Xia Limin, Luo Min, and Yan Wei
- Subjects
Liver Cirrhosis ,Male ,Carcinoma, Hepatocellular ,Cellular differentiation ,education ,Cell ,Blotting, Western ,Kruppel-Like Transcription Factors ,behavioral disciplines and activities ,Metastasis ,Proto-Oncogene Proteins ,medicine ,Kruppel-Like Factor 6 ,Gene silencing ,Humans ,Protein Isoforms ,Gene Silencing ,RNA, Messenger ,Regulation of gene expression ,Venous Thrombosis ,Hepatology ,business.industry ,Reverse Transcriptase Polymerase Chain Reaction ,Tumor Suppressor Proteins ,Liver Neoplasms ,Gastroenterology ,Cell Differentiation ,General Medicine ,Hep G2 Cells ,Middle Aged ,medicine.disease ,Immunohistochemistry ,Tumor Burden ,Gene Expression Regulation, Neoplastic ,Real-time polymerase chain reaction ,medicine.anatomical_structure ,KLF6 ,Lymphatic Metastasis ,Cancer research ,Female ,business - Abstract
BACKGROUND/AIMS This study aimed to detect the expression of newly discovered zinc finger transcriptional factor KLF6 and its splice variant KLF6 SV2 in primary hepatocarcinoma (PHC) tissues and hepatoma cell strains, and to evaluate their clinicopathologic relationship with PHC. METHODOLOGY Wild-type KLF6 and KLF6 SV2 mRNA expression was determined by RTPCR in 27 cases of PHC tissues and cell strains of HepG2, SMMC7721 and LO2. Western blotting and immunohistochemical staining were adopted to detect KLF6 protein expression. Positive area ratio of wild-type KLF6 protein expression and its relationship with clinicopathological parameters of PHC was analyzed. RESULTS Wild-type KLF6 expression in PHC tissues was lower than that in paracancerous tissues. In contrast, KLF6 SV2 mRNA expression was higher in PHC tissues and hepatoma cell strains (p
- Published
- 2011