31 results on '"Silva, Marcelo"'
Search Results
2. A stronger antibody response in increased disease severity of SARS-CoV-2
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de Oliveira, Marta Iglis, Aciole, Melayne Rocha, Neves, Patrícia Areias Feitosa, Silva, Vitor Palmares Oliveira e, Silva, Marcelo Palmares Oliveira e, de Lorena, Virginia Maria Barros, and de Araújo, Paulo Sérgio Ramos
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- 2024
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3. Physical activity trajectory in the first 10 months of the COVID-19 pandemic in Southern Brazil: a follow-up study
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Caputo, Eduardo L., Feter, Natan, Leite, Jayne S., Doring, Igor R., Cassuriaga, Júlia, Delpino, Felipe M., Huckembeck, Caroline M., Alt, Ricardo, da Silva, Marcelo C., Rombaldi, Airton J., and Reichert, Felipe F.
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- 2022
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4. Time series evaluation of condemnation at poultry slaughterhouses enable to export in Southeastern Brazil (2009–2019): a tool for optimizing resources in the poultry production chain
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Hortêncio, Maria Carolina, Costa, Letícia Roberta Martins, de Souza, Maria Victória Pereira, de Freitas, Weslley Domenicci, Fonseca, Belchiolina Beatriz, Silva, Marcelo José Barbosa, and Cossi, Marcus Vinícius Coutinho
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- 2022
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5. Are leisure-time and work-related activities associated with low back pain during pregnancy?
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Caputo, Eduardo L., Domingues, Marlos R., Bertoldi, Andrea D., Ferreira, Paulo H., Ferreira, Manuela L., Shirley, Debra, and da Silva, Marcelo C.
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- 2021
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6. Global, regional, and national trends and patterns in physical activity research since 1950: a systematic review
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Ramírez Varela, Andrea, Cruz, Gloria Isabel Nino, Hallal, Pedro, Blumenberg, Cauane, da Silva, Shana Ginar, Salvo, Deborah, Martins, Rafaela, da Silva, Bruna Gonçalves Cordeiro, Resendiz, Eugen, del Portillo, Maria Catalina, Monteiro, Luciana Zaranza, Khoo, Selina, Chong, Kar Hau, Cozzensa da Silva, Marcelo, Mannocci, Alice, Ding, Ding, and Pratt, Michael
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- 2021
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7. Correction to: Global, regional, and national trends and patterns in physical activity research since 1950: a systematic review
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Varela, Andrea Ramírez, Cruz, Gloria Isabel Nino, Hallal, Pedro, Blumenberg, Cauane, da Silva, Shana Ginar, Salvo, Deborah, Martins, Rafaela, da Silva, Bruna Gonçalves Cordeiro, Resendiz, Eugen, del Portillo, Maria Catalina, Monteiro, Luciana Zaranza, Khoo, Selina, Chong, Kar Hau, da Silva, Marcelo Cozzensa, Mannocci, Alice, Ding, Ding, and Pratt, Michael
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- 2021
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8. Psychopharmacological effects of riparin III from Aniba riparia (Nees) Mez. (Lauraceae) supported by metabolic approach and multivariate data analysis
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Golzio dos Santos, Sócrates, Fernandes Gomes, Isis, Fernandes de Oliveira Golzio, Adriana Maria, Lopes Souto, Augusto, Scotti, Marcus Tullius, Fechine Tavares, Josean, Chavez Gutierrez, Stanley Juan, Nóbrega de Almeida, Reinaldo, Barbosa-Filho, José Maria, and Sobral da Silva, Marcelo
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- 2020
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9. The inclusion of mobilisation with movement to a standard exercise programme for patients with rotator cuff related pain: a randomised, placebo-controlled protocol trial
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Baeske, Rafael, Hall, Toby, and Silva, Marcelo Faria
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- 2020
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10. Care seek behavior for low back pain in southern Brazil during the COVID-19 pandemic: a panel data analysis.
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Caputo, Eduardo L, Feter, Natan, Pinto, Ricardo N C, Delpino, Felipe Mendes, Cassuriaga, Júlia, da Silva, Carine N, Leite, Jayne S, Jerônimo, Jeferson S, da Silva, Sophia D P, Reichert, Felipe F, Rombaldi, Airton J, and da Silva, Marcelo C
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COVID-19 pandemic ,LUMBAR pain ,DATA analysis ,POISSON regression ,REGRESSION analysis ,GENERAL practitioners ,CHIROPRACTORS - Abstract
Background: During the COVID-19 pandemic, people with low back pain (LBP) might have avoided seeking care for their pain. We aimed to investigate how the COVID-19 pandemic has affected LBP care seeking behavior among adults. Methods: Data from four assessments of the PAMPA cohort were analyzed. Participants who reported experiencing LBP during wave one both before and during social restrictions (n = 1,753 and n = 1,712, respectively), wave two (n = 2,009), and wave three (n = 2,482) were included. We asked participants about sociodemographic, behavioral, and health factors and outcomes related to LBP. Poisson regression analyses were conducted, and data are presented as prevalence ratios (PR) and respective 95% confidence interval (95%CI). Results: Overall, care seeking behavior decreased by half in the first months of restrictions, from 51.5% to 25.2%. Although there was an increase in care seeking behavior observed in the other two assessments (nearly 10 and 16 months after restrictions), it was insufficient to reach pre-pandemic levels. In the first months of restrictions, a similar scenario was observed for specific care, such as general practitioner and exercise professional care, with proportions of pre-pandemic levels reached after 10 and 16 months. Women were more likely to seek care for LBP 10 and 16 months after restrictions (PR 1.30 95%CI 1.11; 1.52, PR 1.22 95%CI 1.06; 1.39, respectively). Also, those participants who worked, were physically active, and reported pain-related disability and high pain levels were more likely to seek care at all time points assessed. Conclusion: Overall, care-seeking behavior for LBP significantly decreased in the first months of restrictions and increased in the following months; however, this behavior remained lower than pre-pandemic levels. [ABSTRACT FROM AUTHOR]
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- 2023
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11. Green Tobacco Sickness among Brazilian farm workers and genetic polymorphisms.
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da Mota e Silva, Marcelo Soares, da Glória da Costa Carvalho, Maria, Moreira, Josino Costa, de Oliveira Barreto, Emiliano, de Farias, Karol Fireman, Nascimento, Cristiane Araújo, da Silva, Francisca Maria Nunes, de Andrade, Tiago Gomes, Luiz, Ronir Raggio, de Moura Neto, Rodrigo Soares, and Ribeiro, Fernanda Lattario
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DISEASES in agricultural laborers , *GENETIC polymorphisms , *TOBACCO farms , *PHYSIOLOGICAL effects of nicotine , *AGRICULTURAL laborers - Abstract
Objective: Green Tobacco Sickness (GTS) is an occupational illness caused by dermal absorption of nicotine from tobacco leaves. It affects thousands of farm workers worldwide. Brazil is the second tobacco producer in the world; despite this, there are few studies on GTS among Brazilian harvesters. This study aimed to determine the prevalence of GTS among a population of tobacco workers from a producing area in northeastern Brazil and investigate whether the occurrence of the disease was influenced by factors such age, gender and smoking status. In addition, it was investigated if there was association between the onset of GTS and genetic polymorphisms in genes that encode some detoxification enzymes. A semi-structured questionnaire was used to collect demographic, behavioral and occupational data from the referred workers. Polymorphisms were tested through the Polymerase Chain Reaction technique. Results: The total prevalence of GTS found was 56.9%, with a significant difference between genders (71.7% for women and 35.3% for men, p < 0.0001). No association was identified between the investigated polymorphisms and GTS. This study confirms the occurrence of GTS among tobacco harvesters in Brazil with high prevalence. The investigation suggests the need to take preventive measures to protect tobacco workers against this disease. [ABSTRACT FROM AUTHOR]
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- 2018
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12. Toxicity and antitumor potential of Mesosphaerum sidifolium (Lamiaceae) oil and fenchone, its major component.
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Leite Rolim, Thaísa, Pessoa Meireles, Déborah Ribeiro, Mota Batista, Tatianne, Gomes de Sousa, Tatyanna Kelvia, Mendes Mangueira, Vivianne, Albuquerque de Abrantes, Renata, Lima Rodrigues Pita, João Carlos, Lira Xavier, Aline, Oliveira Costa, Vicente Carlos, Batista, Leônia Maria, Fechine Tavares, Josean, Sobral da Silva, Marcelo, and Vieira Sobral, Marianna
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ANIMAL experimentation ,ANTINEOPLASTIC agents ,CELL cycle ,CLINICAL drug trials ,DRUG toxicity ,ESSENTIAL oils ,GAS chromatography ,HEMOLYSIS & hemolysins ,HISTOLOGICAL techniques ,LIVER diseases ,MASS spectrometry ,MICE ,RESEARCH funding ,SURVIVAL ,PLANT extracts ,PRE-tests & post-tests ,CONTROL groups ,DATA analysis software ,DESCRIPTIVE statistics - Abstract
Background: The essential oil from Mesosphaerum sidifolium (L'Hérit.) Harley & J.F.B.Pastore (syn. Hyptis umbrosa), Lamiaceae (EOM), and its major component, have been tested for toxicity and antitumor activity. Methods: EOM was obtained from aerial parts of M. sidifolium subjected to hydro distillation, and gas chromatography- mass spectrometry was used to characterize the EOM chemical composition. The toxicity was evaluated using haemolysis assay, and acute toxicity and micronucleus tests. Ehrlich ascites carcinoma model was used to evaluate the in vivo antitumor activity and toxicity of EOM (50, 100 and 150 mg/kg), and fenchone (30 and 60 mg/kg) after 9 d of treatment. Results: The EOM major components were fenchone (24.8%), cubebol (6.9%), limonene (5.4%), spathulenol (4.5%), β-caryophyllene (4.6%) and α-cadinol (4.7%). The HC50 (concentration producing 50% haemolysis) was 494.9 μg/mL for EOM and higher than 3000 μg/mL for fenchone. The LD50 for EOM was approximately 500 mg/kg in mice. The essential oil induced increase of micronucleated erythrocytes only at 300 mg/kg, suggesting moderate genotoxicity. EOM (100 or 150 mg/kg) and fenchone (60 mg/kg) reduced all analyzed parameters (tumor volume and mass, and total viable cancer cells). Survival also increased for the treated animals with EOM and fenchone. For EOM 150 mg/kg and 5-FU treatment, most cells were arrested in the G0/G1 phase, whereas for fenchone, cells arrested in the S phase, which represents a blockage in cell cycle progression. Regarding the toxicological evaluation, EOM induced weight loss, but did not induce hematological, biochemical or histological (liver and kidneys) toxicity. Fenchone induceddecreaseofASTandALT,suggestingliverdamage. Conclusions: The data showed EOM caused in vivo cell growth inhibition on Ehrlich ascites carcinoma model by inducing cell cycle arrest, without major changes in the toxicity parameters evaluated. In addition, this activity was associated with the presence of fenchone, its major component. [ABSTRACT FROM AUTHOR]
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- 2017
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13. Stryphnodendron adstringens and purified tannin on Pythium insidiosum: in vitro and in vivo studies.
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Trolezi, Rodrigo, Azanha, Juliana Maziero, Paschoal, Natália Rodrigues, Chechi, Jéssica Luana, Dias Silva, Marcelo José, Fabris, Viciany Eric, Vilegas, Wagner, Kaneno, Ramon, Fernandes Junior, Ary, and de Moraes Gimenes Bosco, Sandra
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OOMYCETES ,PYTHIUM ,COMMUNICABLE diseases ,PATHOGENIC microorganisms ,TANNINS ,LABORATORY rabbits ,ZOOSPORES - Abstract
Background: Pythium insidiosum is the etiological agent of pythiosis, an emerging life-threatening infectious disease in tropical and subtropical regions. The pathogen is a fungus-like organism resistant to antifungal therapy, for this reason, most cases need extensive surgical debridments as treatment, but depending on the size and anatomical region of the lesion, such approach is unfeasible. We investigate the fungicidal effect and toxicity of crude bark extract of Stryphnodendron adstringens and commercially available tannin on Pythium insidiosum both in vitro and in vivo. Methods: Standardized fragments of mycelia of fifteen isolates of P. insidiosum were tested with different concentrations of bark extract (10 to 30% v/v) and tannin (0.5, 1.0 and 1.5 mg/mL). For in vivo study, fifteen rabbits were experimentally infected with zoospores of P. insidiosum and treated by oral and intralesional applications of bark extract and tannin. Acute toxicity tests with both substances were also performed in rats. Results: In vitro studies showed fungicidal effect for both substances at different concentrations and the SEM showed alteration on the cell wall surface of the pathogen. All infected rabbits developed a firm nodular mass that reached around 90 mm
2 ninety days after inoculation, but neither the intralesional inoculation of tannin, nor the oral administration of crude extract and tannin were able to promote remission of the lesions. Conclusions: Lesions developed by rabbits presented an encapsulated abscess being quite different of naturally acquired pythiosis, which is characterized by ulcerated lesions. Since no toxicity was observed in rats or rabbits inoculated with these products, while in vitro experiments showed direct antifungal effect, therapeutic activity of S. adstringens and tannin should be clinically tested as an alternative for healing wounds in naturally acquired pythiosis. [ABSTRACT FROM AUTHOR]- Published
- 2017
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14. Post-exposure serological responses to malaria parasites in potential blood donors.
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Portugal-Calisto, Daniela, Ferreira, Ana Raquel, Silva, Marcelo Sousa, and Teodósio, Rosa
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MALARIA ,BLOOD donors ,PLASMODIUM ,IMMUNOGLOBULINS ,BLOOD transfusion ,DISEASES - Abstract
Background: Cases of transfusion-transmitted malaria have been described around the world and highlighted in some studies. Semi-immune individuals are more likely to transmit malaria as they may be asymptomatic. Some countries allow blood donations only based on epidemiological criteria while others reinforce their criteria with serological tests. However, little is known about the longevity of anti-Plasmodium spp. antibodies and its meaning in blood donation. Therefore, this study aims to assess the longevity of different subclasses of anti-Plasmodium spp. antibodies in individuals with previous stays in endemic areas, as well as to assess how those antibodies are related to personal features and travel characteristics. Based on those results, the suitability of the Portuguese blood donors screening method was addressed, i.e. the method to search for an eventual risk of transfusion-transmitted malaria among the population studied. Results: Statistical associations were found between the presence of total anti-Plasmodium spp. antibodies and some travel characteristics, namely to be born in endemic area versus non endemic and previous episodes of malaria. The intersection between seropositive results and the last year of stay in endemic areas showed a longer longevity of anti-Plasmodium spp. antibodies than previously reported. Those results represented a considerable portion of the individuals having returned from their last stay in endemic areas more than 10 years before enrolment in this study. Considering the study population as potential blood donors, serological results also indicated that if epidemiological criteria alone were applied to screen blood donors, an important percentage of seropositive individuals would be approved for blood donation. Because the nature and meaning of those antibodies in the blood donation context is still not understood, those approved individuals could represent a risk for blood transfusion safety. Conclusions: The place of birth and past episodes of malaria seem to be related to the serological outcome. Epidemiological criteria to screen potential blood donors are insufficient to guarantee the safety of the blood, if applied alone. [ABSTRACT FROM AUTHOR]
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- 2016
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15. Determinants of out-of-pocket health expenditure on children: an analysis of the 2004 Pelotas Birth Cohort.
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Torres da Silva, Marcelo, Barros, Aluísio J. D., Bertoldi, Andréa D., de Andrade Jacinto, Paulo, Matijasevich, Alicia, Santos, Iná S., and Augusto Oviedo Tejada, Cesar
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HEALTH services accessibility , *HEALTH status indicators , *HEALTH insurance , *LONGITUDINAL method , *MEDICAL care costs , *PEDIATRICS , *HEALTH & social status - Abstract
Background: The present study aimed to examine the impact of socioeconomic, demographic, and health status-related factors on out-of-pocket expenditure on health care for children. Methods: Data were obtained from a birth cohort study conducted in the city of Pelotas, state of Rio Grande do Sul (RS), southern Brazil, in 2004. The final sample is a result of adjusts made in order to keep in the analysis only those that attended to 3 follow-ups (at 12, 24 and 48 months of age). Estimates were carried out using the Panel Data Tobit Model with random effects. Results: The study showed that expenditure on medicines was 20 % less likely in those considered healthy children by their mothers and, if there was any expenditure with healthy children, the expected expenditure was reduced by 58 %. A 1 % increase in household income increased the expected expenditure on medicines by 16 %, and by 23 % in children with private health insurance coverage. Conclusions: All types of health care expenditures examined were higher for children covered by private health insurance. Although total health care expenditure was higher for children of better-off families, it represented a lower share of these families' income evidencing income inequality in health care expenditures. [ABSTRACT FROM AUTHOR]
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- 2015
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16. Age-dependent effect of high-fructose and high-fat diets on lipid metabolism and lipid accumulation in liver and kidney of rats.
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Mendes de Castro, Uberdan Guilherme, Dos Santos, Robson Augusto Souza, Eustáquio Silva, Marcelo, De Lima, Wanderson Geraldo, Maria José Campagnole-Santos, Maria José Campagnole-Santos, and Alzamora, Andréia Carvalho
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FRUCTOSE ,HIGH-fat diet ,LIPID metabolism ,LABORATORY rats ,METABOLIC syndrome ,PHYSIOLOGY - Abstract
Background: The metabolic syndrome (MS) is characterized by variable coexistence of metabolic and pathophysiological alterations which are important risk factors for developing of type II diabetes and/or cardiovascular diseases. Increased of MS patients in worldwide has stimulated the development of experimental models. However, it is still challenging to find an dietetic model that most closely approximates human MS and, in addition, is not yet fully established the effect of different diets of MS in lipid metabolism in rats of different ages. The aim of this study was to evaluate the effect of different diets of MS in lipid metabolism and ectopic fat deposition and define the most appropriate diet for inducing the characteristic disturbances of the human MS in rats of different ages. Methods: Young (4 weeks old) and adult rats (12 weeks old) were given a high-fat (FAT) or high-fructose diet (FRU) for 13 weeks and biochemical, physiological, histological and biometric parameters were evaluated. Results: In young rats, the FAT diet induced increased mean blood pressure (MAP) and heart rate (HR), body weight after 6 to 10 weeks, and in the 13th week, increased the liver, mesenteric, retroperitoneal and epididymal fat weights, fasting glucose, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) and reduced HDL cholesterol; and also induced non-alcoholic fatty liver disease (NAFLD) and renal inflammatory infiltrates. In adult rats, the FRU diet induced transient elevations of MAP and HR in the 6th week, and, at 13 weeks, increased fasting glucose, triglycerides, total cholesterol, AST and ALT; increased liver, kidneys and retroperitoneal fat weights; and induced macrovesicular and microvesicular NAFLD, the presence of fat cells in the kidney, glomerular sclerosis, and liver and kidney inflammation. Additionally, the FAT and FRU diets induced, respectively, increases in liver glycogen in adults and young rats. Conclusions: Our data show that FRU diet in adult rats causes biggest change on metabolism of serum lipids and lipid accumulation in liver and kidney, while the FAT diet in young rats induces elevation of MAP and HR and higher increased visceral lipid stores, constituting the best nutritional interventions to induce MS in rats. [ABSTRACT FROM AUTHOR]
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- 2013
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17. Increase of chronic low back pain prevalence in a medium-sized city of southern Brazil.
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Meucci, Rodrigo D., Fassa, Anaclaudia G., Paniz, Vera M. V., Silva, Marcelo C., and Wegman, David H.
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LUMBAR pain ,BACK diseases ,LUMBOSACRAL region ,LUMBAR vertebrae ,ECONOMIC status - Abstract
Background: Chronic low back pain (CLBP) is a highly disabling morbidity with high social, economic and individual effects. Demographic, occupational and behavioral changes that took place in Brazil over the last decade are related with an increasing burden of chronic conditions. Despite these changes, comparison studies on CLBP prevalence and associated factors, over time are scarce in the literature in general, and unknown in Brazil. The present study compared the CLBP prevalence in a medium sized city in Brazil between the years 2002 and 2010 and examined factors associated with prevalence in 2010. Methods: Two cross-sectional studies with similar methodology were conducted in a medium-sized city in southern Brazil, in 2002 and 2010. 3182 individuals were interviewed in the first study and 2732 in the second one, all adults aged twenty years or more. Those who reported pain for seven weeks or more in the last three months in the lumbar region where considered cases of CLBP. Results: The CLBP prevalence increased from 4.2% to 9.6% in 8 years. In most of the studied subgroups the CLBP prevalence has at least doubled and the increase was even larger among younger individuals with more years of education and higher economic status. Conclusions: Increase in CLBP prevalence is worrisome because it is a condition responsible for substantial social impact, besides being an important source of demand for health services. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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18. Immunoproteomic analysis of Plasmodium falciparum antigens using sera from patients with clinical history of imported malaria.
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Costa, Rita M., Nogueira, Fátima, de Sousa, Karina P., Vitorino, Rui, and Silva, Marcelo S.
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PLASMODIUM falciparum ,PARASITE antigens ,MALARIA transmission ,PROTEOMICS ,MALARIA prevention ,ELONGATION factors (Biochemistry) ,ANTIBODY formation - Abstract
Background: The malaria caused by Plasmodium falciparum remains a serious public health problem in the world, due largely to the absence of an effective vaccine. There is a lack of information on the structural properties and antigens capable of activating the immunological mechanisms for the induction of protective immunity. Therefore, the objective of this study is to evaluate the serological reactivity of sera from individuals with imported malaria and identify major immunogenic proteins. Methods: The study was conducted in 227 individuals with imported malaria and 23 healthy individuals who had never been in areas endemic for malaria. The determination of anti-P. falciparum IgG antibodies was performed by an ELISA validated and optimized for this study. Sera showing higher reactivity to anti-P. falciparum by ELISA were analysed by immunoblotting and immunogenic proteins were identified by mass spectroscopy. Results: The results of anti-P. falciparum antibodies research by ELISA indicates 78 positive, 137 negative and 12 indeterminate sera. Analysis of immunoblotting demonstrated a consistent pattern with respect to immunoreactivity of antigens with molecular weights in the range of 40 to 60 kDa. Between 40 and 60 kDa six immunogenic proteins were identified: elongation factor-1 alpha (EF-1α), protein disulphide isomerase (PDI); phosphoglycerate kinase (PGK); 78 kDa glucose-regulated protein homologue (GRP-78); rhoptry-associated protein 2 (RAP-2) and rhoptry-associated protein 3 (RAP-3). Conclusions: It was identified immunogenic proteins essential for parasite survival in the host, two of which (RAP-2 and RAP-3) are already described in the literature as proteins that play an important role in the invasion of erythrocytes by extracellular merozoites. [ABSTRACT FROM AUTHOR]
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- 2013
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19. Inoculation of Triatoma Virus (Dicistroviridae: Cripavirus) elicits a non-infective immune response in mice.
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Querido, Jailson F. B., Agirre, Jon, Marti, Gerardo A., Guérin, Diego M. A., and Silva, Marcelo Sousa
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TRIATOMA ,ASSASSIN bugs ,CONENOSES ,TRIATOMA lecticularius ,IMMUNE response ,LABORATORY mice - Abstract
Background: Dicistroviridae is a new family of small, non-enveloped, +ssRNA viruses pathogenic to both beneficial arthropods and insect pests. Little is known about the dicistrovirus replication mechanism or gene function, and any knowledge on these subjects comes mainly from comparisons with mammalian viruses from the Picornaviridae family. Due to its peculiar genome organization and characteristics of the per os viral transmission route, dicistroviruses make good candidates for use as biopesticides. Triatoma virus (TrV) is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of the human trypanosomiasis disease called Chagas disease. TrV was postulated as a potential control agent against Chagas' vectors. Although there is no evidence that TrV nor other dicistroviruses replicate in species outside the Insecta class, the innocuousness of these viruses in humans and animals needs to be ascertained. Methods: In this study, RT-PCR and ELISA were used to detect the infectivity of this virus in Mus musculus BALB/c mice. Results: In this study we have observed that there is no significant difference in the ratio IgG2a/IgG1 in sera from animals inoculated with TrV when compared with non-inoculated animals or mice inoculated only with non-infective TrV protein capsids. Conclusions: We conclude that, under our experimental conditions, TrV is unable to replicate inmice. This study constitutes the first test to evaluate the infectivity of a dicistrovirus in a vertebrate animal model. [ABSTRACT FROM AUTHOR]
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- 2013
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20. The antinociceptive effect of electroacupuncture at different depths of acupoints and under the needling surface.
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Silva, Marcelo L., Silva, Josie R.T., and Prado, Wiliam A.
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ELECTROACUPUNCTURE methodology , *ACUPUNCTURE , *ACUPUNCTURE points , *ANALGESIA , *ANALYSIS of variance , *ANIMAL experimentation , *EXPERIMENTAL design , *FORMALDEHYDE , *HYPODERMIC needles , *MULTIVARIATE analysis , *NOCICEPTORS , *PHYSIOLOGIC salines , *RATS , *RESEARCH funding , *STATISTICAL sampling , *STATISTICS , *DATA analysis , *QI (Chinese philosophy) , *PAIN measurement , *REPEATED measures design , *DATA analysis software , *DESCRIPTIVE statistics - Abstract
Background: The stimulation of acupoints along the meridians, but not the non-acupoints outside of the meridians, produces analgesia. Although the acupoint is defined at the body surface, the exact location of the acupoints is not known. This study aims to examine whether the intensity and duration of the analgesic effect of electroacupuncture (EA) at the Zusanli (ST36) and Sanynjiao acupoints (SP6) change according to the depth of the stimulation. Methods: Ninety-six male Wistar rats classified as responders were arbitrarily allocated into 16 groups of six rats each. Six groups received EA with uninsulated acupuncture needles (type I) or needles that were immersed in varnish and had the varnish circularly peeled 0.2 mm from the tip (type II), 0.2 mm at 3 mm (type III) or 5 mm (type IV) from the tip, or 0.2 mm at 5 and 1 mm from the tip (type V), or EA sham for 20 min. Five groups received injection of formalin into the acupoint bilaterally at 5 mm or 1 mm deep into ST36, 5 mm below ST36 but inserting the needle at 45° to the skin surface, or 5 mm deep into non-acupoints. The remaining groups received intraplantar injection of saline, 1% or 2.5% formalin. The analgesic effects were measured by the rat tail-flick test. Results: The bilateral stimulation of ST36 and SP6 by uninsulated or insulated needles produced analgesia in the rat tail-flick test. The stronger and longer lasting effects occurred after EA with the types I and V needles, or injection of formalin 5 mm deep into ST36. The remaining needles produced weaker and shorter lasting effects. Slow analgesic effect also occurred after formalin injection at 1 mm or 5 mm below ST36 by inserting the needle at 45° to the skin surface. Conclusion: The experimental results suggest that the efficacy of the EA stimulation depends on the spatial distribution of the current density under the needling surface rather than only the acupoint or the depth of needling. [ABSTRACT FROM AUTHOR]
- Published
- 2012
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21. Salt overload in fructose-fed insulin-resistant rats decreases paraoxonase-1 activity.
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Cláudia Dornas, Waleska, Geraldo de Lima, Wanderson, Cardoso dos Santos, Rinaldo, Oliveira de Souza, Melina, Silva, Maísa, Fiuza Diniz, Mirla, and Eustáquio Silva, Marcelo
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ANALYSIS of variance ,ANIMAL experimentation ,CARDIOVASCULAR diseases risk factors ,STATISTICAL correlation ,ESTERASES ,FRUCTOSE ,HIGH density lipoproteins ,INSULIN resistance ,LIPIDS ,LOW density lipoproteins ,LYMPHOCYTES ,MONOCYTES ,PROTEINS ,RATS ,SALT ,STATISTICAL sampling ,SPECTROPHOTOMETRY ,STATISTICS ,DATA analysis ,ALBUMINS ,DESCRIPTIVE statistics ,LEUKOCYTE count - Abstract
Paraoxonase 1 (PON1) is a HDL-associated esterase/lactonase and its activity is inversely related to the risk of cardiovascular diseases. The aim of the present study was to evaluate the effect of a high-salt diet on serum PON1 activity in fructose-fed insulin-resistant rats. Adult male Fischer rats were initially divided into two groups. Control (CON), which received a normal salt diet and drinking water throughout the study; high fructose (HF), which received a normal salt diet and 20% fructose supplemented drinking water. After 10 weeks, half of the animals from HF group were randomly switched to a high-salt diet and 20% fructose supplemented drinking water (HFS) for more 10 weeks. Serum PON1 activity was determined by synthetic substrate phenyl acetate. HFS rats showed markedly decreased PON1 activity (HFS rats, 44.3 ± 14.4 g/dL versus CON rats, 64.4 ± 13.3 g/dL, P<0.05) as compared to controls. In parallel, the level of oxidative stress, as indicated by thiobarbituric acid reactive substances (TBARS), was increased in HFS rats by 1.2-fold in the liver in relation to controls and was negatively correlated with PON activity. Differential leukocyte counts in blood showed a significant change in lymphocytes and monocytes profile. In conclusion, these results show that PON1 activity is decreased in fructose-fed insulin-resistant rats on a high-salt diet, which may be associated with increased oxidative stress, leading to inflammation. [ABSTRACT FROM AUTHOR]
- Published
- 2012
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22. The Leishmania amazonensis TRF (TTAGGG repeat-binding factor) homologue binds and co-localizes with telomeres.
- Author
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da Silva, Marcelo S., Perez, Arina M., de Cássia V. da Silveira, Rita, de Moraes, Camila E., Siqueira-Neto, Jair L., de H. Freitas, Junior, Lucio, and Cano, Maria Isabel N.
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LEISHMANIA , *CHROMOSOMES , *GENES , *PROTEINS , *DNA , *ENZYMES - Abstract
Background: Telomeres are specialized structures at the end of chromosomes essential for maintaining genome stability and cell viability. The importance of telomeric proteins for telomere maintenance has increased our interest in the identification of homologues within the genus Leishmania. The mammalian TRF1 and TRF2 proteins, for example, bind double-stranded telomeres via a Myb-like DNA-binding domain and are involved with telomere length regulation and chromosome end protection. In addition, TRF2 can modulate the activity of several enzymes and influence the conformation of telomeric DNA. In this work, we identified and characterized a Leishmania protein (LaTRF) homologous to both mammalian TRF1 and TRF2. Results: LaTRF was cloned using a PCR-based strategy. ClustalW and bl2seq sequence analysis showed that LaTRF shared sequence identity with the Trypanosoma brucei TRF (TbTRF) protein and had the same degree of sequence similarities with the dimerization (TRFH) and the canonical DNA-binding Myb-like domains of both mammalian TRFs. LaTRF was predicted to be an 82.5 kDa protein, indicating that it is double the size of the trypanosome TRF homologues. Western blot and indirect immunofluorescence combined with fluorescence in situ hybridization showed that LaTRF, similarly to hTRF2, is a nuclear protein that also associates with parasite telomeres. Native and full length LaTRF and a mutant bearing the putative Myb-like domain expressed in bacteria bound double-stranded telomeric DNA in vitro. Chromatin immunoprecipitation showed that LaTRF interacted specifically with telomeres in vivo. Conclusion: The nuclear localization of LaTRF, its association and co-localization with parasite telomeres and its high identity with TbTRF protein, support the hypothesis that LaTRF is a Leishmania telomeric protein. [ABSTRACT FROM AUTHOR]
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- 2010
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23. Immunotherapy for liver tumors: present status and future prospects.
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Matar, Pablo, Alaniz, Laura, Rozados, Viviana, Aquino, Jorge B, Malvicini, Mariana, Atorrasagasti, Catalina, Gidekel, Manuel, Silva, Marcelo, Scharovsky, O Graciela, and Mazzolini, Guillermo
- Subjects
LIVER cancer ,IMMUNE system ,CLINICAL trials ,IMMUNOTHERAPY ,MEDICAL experimentation on humans - Abstract
Increasing evidence suggests that immune responses are involved in the control of cancer and that the immune system can be manipulated in different ways to recognize and attack tumors. Progress in immune-based strategies has opened new therapeutic avenues using a number of techniques destined to eliminate malignant cells. In the present review, we overview current knowledge on the importance, successes and difficulties of immunotherapy in liver tumors, including preclinical data available in animal models and information from clinical trials carried out during the lasts years. This review shows that new options for the treatment of advanced liver tumors are urgently needed and that there is a ground for future advances in the field. [ABSTRACT FROM AUTHOR]
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- 2009
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24. Minor psychiatric disorders among Brazilian ragpickers: a cross-sectional study.
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Da Silva, Marcelo Cozzensa, Fassa, Anaclaudia Gastal, and Kriebel, David
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PERINATAL mood & anxiety disorders , *RAGPICKERS , *BRAZILIANS , *OCCUPATIONAL diseases , *COHORT analysis - Abstract
Background: Ragpickers are informal workers who collect recyclable materials to earn a small wage. Their life and working conditions are extremely difficult. We examined minor psychiatric disorders (MPD) among a cohort of ragpickers in Pelotas, a city in southern Brazil. Methods: Ragpickers were matched by sex, age, and years of schooling with a sample of nonragpickers from the same poor neighborhoods. The cross-sectional study gathered data by interview on 990 individuals in 2004. MPD were assessed using a standard self-reporting questionnaire, the SRQ-20. Results: The prevalence of MPD among ragpickers was 44.7%, higher than reported by neighborhood controls (33.6%; p < 0.001). MPD were more common among females, those of lower economic level, smokers and alcoholics. Among occupational characteristics, MPD prevalence was associated with frequent static postures, low job satisfaction and recent work accidents. Conclusion: Ragpickers more frequently report MPD than other poor workers living in the same neighborhoods, with many of the same life conditions. Improving the work lives of these precarious workers should address not only the physical hazards of their jobs but their mental and emotional health as well. [ABSTRACT FROM AUTHOR]
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- 2006
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25. Use of quantum dots of sizes magic in biological systems.
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Deus, Samantha, Luz, Felipe, Silva, Anielle, Dantas, Noelio, and Silva, Marcelo
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QUANTUM dots ,BIOLOGICAL systems - Abstract
An abstract of the article "Use of quantum dots of sizes magic in biological systems" by Samantha Deus and colleagues is presented.
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- 2014
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26. Erratum to: toxicity and antitumor potential of Mesosphaerum sidifolium (Lamiaceae) oil and fenchone, its major component.
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Rolim TL, Meireles DRP, Batista TM, de Sousa TKG, Mangueira VM, de Abrantes RA, Pita JCLR, Xavier AL, Costa VCO, Batista LM, da Silva MS, and Sobral MV
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- 2017
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27. Toxicity and antitumor potential of Mesosphaerum sidifolium (Lamiaceae) oil and fenchone, its major component.
- Author
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Rolim TL, Meireles DRP, Batista TM, de Sousa TKG, Mangueira VM, de Abrantes RA, Pita JCLR, Xavier AL, Costa VCO, Batista LM, da Silva MS, and Sobral MV
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- Animals, Antineoplastic Agents, Phytogenic chemistry, Antineoplastic Agents, Phytogenic toxicity, Camphanes, Carcinoma, Ehrlich Tumor physiopathology, Cell Cycle Checkpoints drug effects, Cell Proliferation drug effects, Female, Humans, Mice, Norbornanes chemistry, Norbornanes toxicity, Oils, Volatile chemistry, Oils, Volatile toxicity, Plant Oils chemistry, Plant Oils toxicity, Antineoplastic Agents, Phytogenic administration & dosage, Carcinoma, Ehrlich Tumor drug therapy, Lamiaceae chemistry, Norbornanes administration & dosage, Oils, Volatile administration & dosage, Plant Oils administration & dosage
- Abstract
Background: The essential oil from Mesosphaerum sidifolium (L'Hérit.) Harley & J.F.B.Pastore (syn. Hyptis umbrosa), Lamiaceae (EOM), and its major component, have been tested for toxicity and antitumor activity., Methods: EOM was obtained from aerial parts of M. sidifolium subjected to hydro distillation, and gas chromatography-mass spectrometry was used to characterize the EOM chemical composition. The toxicity was evaluated using haemolysis assay, and acute toxicity and micronucleus tests. Ehrlich ascites carcinoma model was used to evaluate the in vivo antitumor activity and toxicity of EOM (50, 100 and 150 mg/kg), and fenchone (30 and 60 mg/kg) after 9 d of treatment., Results: The EOM major components were fenchone (24.8%), cubebol (6.9%), limonene (5.4%), spathulenol (4.5%), β-caryophyllene (4.6%) and α-cadinol (4.7%). The HC50 (concentration producing 50% haemolysis) was 494.9 μg/mL for EOM and higher than 3000 μg/mL for fenchone. The LD50 for EOM was approximately 500 mg/kg in mice. The essential oil induced increase of micronucleated erythrocytes only at 300 mg/kg, suggesting moderate genotoxicity. EOM (100 or 150 mg/kg) and fenchone (60 mg/kg) reduced all analyzed parameters (tumor volume and mass, and total viable cancer cells). Survival also increased for the treated animals with EOM and fenchone. For EOM 150 mg/kg and 5-FU treatment, most cells were arrested in the G0/G1 phase, whereas for fenchone, cells arrested in the S phase, which represents a blockage in cell cycle progression. Regarding the toxicological evaluation, EOM induced weight loss, but did not induce hematological, biochemical or histological (liver and kidneys) toxicity. Fenchone induced decrease of AST and ALT, suggesting liver damage., Conclusions: The data showed EOM caused in vivo cell growth inhibition on Ehrlich ascites carcinoma model by inducing cell cycle arrest, without major changes in the toxicity parameters evaluated. In addition, this activity was associated with the presence of fenchone, its major component.
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- 2017
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28. Determinants of out-of-pocket health expenditure on children: an analysis of the 2004 Pelotas Birth Cohort.
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da Silva MT, Barros AJ, Bertoldi AD, de Andrade Jacinto P, Matijasevich A, Santos IS, and Tejada CA
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- Brazil, Child, Child, Preschool, Cohort Studies, Humans, Infant, Surveys and Questionnaires, Child Health statistics & numerical data, Financing, Personal economics, Health Expenditures statistics & numerical data, Insurance, Health economics
- Abstract
Background: The present study aimed to examine the impact of socioeconomic, demographic, and health status-related factors on out-of-pocket expenditure on health care for children., Methods: Data were obtained from a birth cohort study conducted in the city of Pelotas, state of Rio Grande do Sul (RS), southern Brazil, in 2004. The final sample is a result of adjusts made in order to keep in the analysis only those that attended to 3 follow-ups (at 12, 24 and 48 months of age). Estimates were carried out using the Panel Data Tobit Model with random effects., Results: The study showed that expenditure on medicines was 20 % less likely in those considered healthy children by their mothers and, if there was any expenditure with healthy children, the expected expenditure was reduced by 58 %. A 1 % increase in household income increased the expected expenditure on medicines by 16 %, and by 23 % in children with private health insurance coverage., Conclusions: All types of health care expenditures examined were higher for children covered by private health insurance. Although total health care expenditure was higher for children of better-off families, it represented a lower share of these families' income evidencing income inequality in health care expenditures.
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- 2015
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29. Seroprevalence of Triatoma virus (Dicistroviridae: Cripaviridae) antibodies in Chagas disease patients.
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Querido JF, Echeverría MG, Marti GA, Costa RM, Susevich ML, Rabinovich JE, Copa A, Montaño NA, Garcia L, Cordova M, Torrico F, Sánchez-Eugenia R, Sánchez-Magraner L, Muñiz-Trabudua X, López-Marijuan I, Rozas-Dennis GS, Diosque P, de Castro AM, Robello C, Rodríguez JS, Altcheh J, Salazar-Schettino PM, Bucio MI, Espinoza B, Guérin DM, and Silva MS
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- Americas epidemiology, Animals, Chagas Disease epidemiology, Chagas Disease immunology, Enzyme-Linked Immunosorbent Assay methods, Humans, Models, Biological, Portugal epidemiology, Seroepidemiologic Studies, Viral Structural Proteins immunology, Antibodies, Viral blood, Chagas Disease blood, Dicistroviridae immunology, Triatoma virology
- Abstract
Background: Chagas disease is caused by Trypanosoma cruzi, and humans acquire the parasite by exposure to contaminated feces from hematophagous insect vectors known as triatomines. Triatoma virus (TrV) is the sole viral pathogen of triatomines, and is transmitted among insects through the fecal-oral route and, as it happens with T. cruzi, the infected insects release the virus when defecating during or after blood uptake., Methods: In this work, we analysed the occurrence of anti-TrV antibodies in human sera from Chagas disease endemic and non-endemic countries, and developed a mathematical model to estimate the transmission probability of TrV from insects to man, which ranged between 0.00053 and 0.0015., Results: Our results confirm that people with Chagas disease living in Bolivia, Argentina and Mexico have been exposed to TrV, and that TrV is unable to replicate in human hosts., Conclusions: We presented the first experimental evidence of antibodies against TrV structural proteins in human sera.
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- 2015
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30. Age-dependent effect of high-fructose and high-fat diets on lipid metabolism and lipid accumulation in liver and kidney of rats.
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de Castro UG, dos Santos RA, Silva ME, de Lima WG, Campagnole-Santos MJ, and Alzamora AC
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- Adipose Tissue drug effects, Age Factors, Animals, Blood Pressure drug effects, Body Weight drug effects, Dietary Fats adverse effects, Disease Models, Animal, Fatty Liver etiology, Fatty Liver pathology, Heart Rate drug effects, Humans, Kidney drug effects, Kidney pathology, Lipid Metabolism drug effects, Lipoproteins blood, Liver drug effects, Liver pathology, Male, Metabolic Syndrome etiology, Metabolic Syndrome pathology, Non-alcoholic Fatty Liver Disease, Rats, Rats, Inbred F344, Triglycerides blood, Diet, High-Fat, Fatty Liver metabolism, Fructose administration & dosage, Kidney metabolism, Liver metabolism, Metabolic Syndrome metabolism
- Abstract
Background: The metabolic syndrome (MS) is characterized by variable coexistence of metabolic and pathophysiological alterations which are important risk factors for developing of type II diabetes and/or cardiovascular diseases. Increased of MS patients in worldwide has stimulated the development of experimental models. However, it is still challenging to find an dietetic model that most closely approximates human MS and, in addition, is not yet fully established the effect of different diets of MS in lipid metabolism in rats of different ages. The aim of this study was to evaluate the effect of different diets of MS in lipid metabolism and ectopic fat deposition and define the most appropriate diet for inducing the characteristic disturbances of the human MS in rats of different ages., Methods: Young (4 weeks old) and adult rats (12 weeks old) were given a high-fat (FAT) or high-fructose diet (FRU) for 13 weeks and biochemical, physiological, histological and biometric parameters were evaluated., Results: In young rats, the FAT diet induced increased mean blood pressure (MAP) and heart rate (HR), body weight after 6 to 10 weeks, and in the 13th week, increased the liver, mesenteric, retroperitoneal and epididymal fat weights, fasting glucose, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) and reduced HDL cholesterol; and also induced non-alcoholic fatty liver disease (NAFLD) and renal inflammatory infiltrates. In adult rats, the FRU diet induced transient elevations of MAP and HR in the 6th week, and, at 13 weeks, increased fasting glucose, triglycerides, total cholesterol, AST and ALT; increased liver, kidneys and retroperitoneal fat weights; and induced macrovesicular and microvesicular NAFLD, the presence of fat cells in the kidney, glomerular sclerosis, and liver and kidney inflammation. Additionally, the FAT and FRU diets induced, respectively, increases in liver glycogen in adults and young rats., Conclusions: Our data show that FRU diet in adult rats causes biggest change on metabolism of serum lipids and lipid accumulation in liver and kidney, while the FAT diet in young rats induces elevation of MAP and HR and higher increased visceral lipid stores, constituting the best nutritional interventions to induce MS in rats.
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- 2013
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31. Salt overload in fructose-fed insulin-resistant rats decreases paraoxonase-1 activity.
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Dornas WC, de Lima WG, Dos Santos RC, de Souza MO, Silva M, Diniz MF, and Silva ME
- Abstract
Paraoxonase 1 (PON1) is a HDL-associated esterase/lactonase and its activity is inversely related to the risk of cardiovascular diseases. The aim of the present study was to evaluate the effect of a high-salt diet on serum PON1 activity in fructose-fed insulin-resistant rats. Adult male Fischer rats were initially divided into two groups. Control (CON), which received a normal salt diet and drinking water throughout the study; high fructose (HF), which received a normal salt diet and 20% fructose supplemented drinking water. After 10 weeks, half of the animals from HF group were randomly switched to a high-salt diet and 20% fructose supplemented drinking water (HFS) for more 10 weeks. Serum PON1 activity was determined by synthetic substrate phenyl acetate. HFS rats showed markedly decreased PON1 activity (HFS rats, 44.3 ± 14.4 g/dL versus CON rats, 64.4 ± 13.3 g/dL, P < 0.05) as compared to controls. In parallel, the level of oxidative stress, as indicated by thiobarbituric acid reactive substances (TBARS), was increased in HFS rats by 1.2-fold in the liver in relation to controls and was negatively correlated with PON activity. Differential leukocyte counts in blood showed a significant change in lymphocytes and monocytes profile. In conclusion, these results show that PON1 activity is decreased in fructose-fed insulin-resistant rats on a high-salt diet, which may be associated with increased oxidative stress, leading to inflammation.
- Published
- 2012
- Full Text
- View/download PDF
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