1. Pharmacological inhibition of AKT sensitizes MCF-7 human breast cancer-initiating cells to radiation.
- Author
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Zhan, Jun-Fang, Wu, Liang-Ping, Chen, Long-Hua, Yuan, Ya-Wei, Xie, Guo-Zhu, Sun, Ai-Min, Liu, Ying, and Chen, Zhi-Xian
- Subjects
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BREAST cancer , *STEM cells , *CANCER cells , *CANCER radiotherapy , *CANCER treatment , *APOPTOSIS - Abstract
Background: Caner-initiating cells (CICs or cancer stem cells) have been shown both experimentally and clinically to be resistant to radiation. The mechanism underlying radioresistance remains unclear. Methods: In the present study, we screened 51 genes which are potentially important in mediating radioresistance of breast CICs. Results: The expression of AKT1 and AKT2 at protein and mRNA levels was dramatically increased among the screened genes by 8 Gy radiation treatment in MCF-7 mammosphere cells (predominantly CD24/CD44 CICs), but not in the bulk population of MCF-7 cells (predominantly CD24/CD44). Using apoptosis and clonogenic survival assays, we found pharmacological inhibition of AKT with selective inhibitors of AKT sensitized MCF-7 mammosphere cells, but not MCF-7 monolayer cells to radiation. Conclusion: The present findings suggest that treatment with AKT inhibitors prior to ionizing radiation treatment may be a potential benefit to patients with breast cancer, in particular to eradiate breast CICs. [ABSTRACT FROM AUTHOR]
- Published
- 2011
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