Search

The study aimed to monitor the prevalence of ectoparasites and bacterial infestations in carp and to determine effective dosages of a new iodine-based treatment for disease prevention. The research found that carp were affected by ectoparasites G. kobayashii at 43.32%, L. cyprinacea at 46.67%, and P. lusiana at 31.71% in fish farming facilities within the Sumy Region. Treatment with the iodine-based product resulted in a reduction of infestation levels to 0.2%, including a decrease in the incidence of gyrodactylosis by 89.92-97.35%, lernaeosis by 79.13-95.91%, and philometroidosis by 81.30-91.16% compared to monitoring results. In investigations of bacterial infestations, it was found that the contamination of carp was higher for E. coli at the PC “Shmatukha” by 8.0%, Aeromonas hydrophila by 47.97%, and Aeromonas salmonicida by 31.39%. Additionally, Pseudomonas anguilliseptica was detected at 19.48%, Streptococcus iniae at 13.27%, and Micrococcus spp. at 38.14% at LLC “Ryasnyanske”. The presence of Staphylococcus aureus was recorded at 21.37%, and Listeria monocytogenes at 11.43% at LLC “Bdzhola”; these figures were lower than those observed in other farms. Following treatment with the iodine-based agent, contamination of fish by E. coli decreased by 98.96% at the PC “Shmatukha”, 99.2% at LLC “Bdzhola”, and 84.32% at LLC “Ryasnyanske”. Contamination of carp by A. hydrophila decreased by 94.51% at the PC “Shmatukha”, 80.28% at LLC “Bdzhola”, and 99.15% at LLC “Ryasnyanske”. The presence of A. salmonicida was reduced by 97.23% at the PC “Shmatukha”, 94.00% at LLC “Bdzhola”, and 79.06% at LLC “Ryasnyanske”. Contamination by S. aureus decreased by 92.15% at the PC “Shmatukha”, 98.74% at LLC “Bdzhola”, and 93.89% at LLC “Ryasnyanske”. The levels of S. iniae decreased by 80.90% at the PC “Shmatukha”, 99.63% at LLC “Bdzhola”, and 83.30% at LLC “Ryasnyanske”. Contamination by L. monocytogenes was reduced by 96.25% at the PC “Shmatukha”, 96.08% at LLC “Bdzhola”, and 98.13% at LLC “Ryasnyanske”. Furthermore, Micrococcus spp. levels decreased by 93.74% at the PC “Shmatukha”, 96.37% at LLC “Bdzhola”, and 92.26% at LLC “Ryasnyanske”, in comparison to the monitoring results. The practical value of this study lies in controlling the spread of ectoparasites and developing preventive measures to eliminate ectoparasites and bacterial infestations in carp prior to their release into water bodies

In poultry rearing on deep bedding systems, microorganisms that cause infectious diseases develop within the bedding. This study aimed to determine the physical properties and microbial accumulation capacity of different types of bedding. Methods used included thermo-programmed desorption mass spectrometry, microbiological methods, and scanning electron microscopy. The hygroscopic properties of straw bedding deteriorated by 269.90% from the seventh to the fortysecond day. In the experimental room, where a dry disinfectant was added to the straw bedding, the hygroscopic capacity was 86.70% at the end of the experiment, a difference of 183.2%. Wood shavings bedding, with the addition of disinfectant, exhibited a water absorption capacity 102.82% higher than the control on the forty-second day. Granulated bedding with disinfectant retained moisture 150.33% better compared to the control. At the end of the experiment, the pH of straw bedding was 8.13 in the control group compared to 7.56 in the treated group; for wood shavings was 7.95 versus 7.16; and for granule was 7.35 versus 6.35, due to the use of the disinfectant. At the conclusion of the experiment, granulated bedding exhibited the lowest pH compared to straw and wood shavings, affecting the growth and reproduction of microorganisms. Monitoring of microorganisms in the bedding revealed the presence of bacteria: Escherichia coli, Salmonella typhimurium, Salmonella pullorum, Clostridium perfringens, Staphylococcus aureus, Klebsiella pneumonia, Listeria monocytogenes, as well as microscopic fungi: Fusarium sporotrichioides, Aspergillus niger, and Eimeria oocysts. The use of disinfectant reduced bacterial contamination of straw bedding by 12.35-199.19%, fungal contamination by 633.91-1959.14%, and Eimeria by 676.50%. In the wood shavings bedding, bacterial contamination was reduced by 32.84-257.93%, fungal contamination by 487.08-1098.4%, and Eimeria by 570.21%. Granulated bedding showed reduced bacterial accumulation by 50.93-228.87%, fungal accumulation by 169.89-500.9%, and Eimeria by 301.56%. The practical significance of this study lies in the improved physical properties of poultry bedding and the reduction in bacterial, fungal, and Eimeria oocyst accumulation

Antibacterial preparations are used to prevent bacterial diseases in poultry when raising broilers but given the negative factor of their residual accumulation in meat and the acquisition of resistance by pathogens, it became necessary to find alternative means. The purpose of this study was to determine the effectiveness of various concentrations of Bacillus coagulans on the growth and development of broiler chickens. Methods employed: microbiological; physiological to determine the state of health and safety of chickens; zootechnical; pathological; statistical. The chickens in the experiment had a higher live weight at Day 35: in Group 1 – by 11%, in Group 2 – by 15.4%, and in Group 3 – by 18.4%, as opposed to the control. The average daily body weight gain of chickens in groups with B. coagulans was higher, in Group 1–by 10.8%, in Group 2–by 15%, and in Group 3–by 18.3%. The preservation rate in all experimental groups, regardless of the probiotic concentration, was 100%, while in the control group – 80%. There was an increase in live weight in the following groups: Group 1–by 11%, Group 2 – by 15.5%, Group 3 – by 19%. Feed conversion was lower in Group 1 by 5.3%, in Group 2 – by 3.4%, and in Group 3 – by 2%, compared to the control. At the end of the study, the level of Lactobacillus sp. in the intestines of chickens in Group 1 was 33.78% higher, in Group 2 – by 50%, in Group 3 – by 78.37%; a decrease in the content of Enterobacteriaceae sp. in Group 1–by 51.48%, in Group 2 – by 65.11%, in Group 3–by 90.67%; Staphylococcus sp. in Group 1–by 15.04%, in Group 2–by 35.44%, in Group 3–by 51.47% (p≤0.05), in contrast to the control. The average bursal weight in Group 1 was 4.82% higher, in Group 2–30% higher, in Group 3–37.53% higher, and the bursal index was 15%, 25%, and 30% higher, respectively, compared to the control

Salmonella pullorum (S. pullorum) is the causative agent of pullorum disease and results in severe economic losses in poultry, and can long-term survival by colonizing host organs. steE is an effector protein secreted by Salmonella pathogenicity island 2. It is not clear in vivo for the colonization of Salmonella. To investigate the role of steE on the colonization of S. Pullorum in the principal organs of chicken, we used S. pullorum and S. pullorum ΔsteE strains immunized chickens, respectively. The results of the virulence assay showed that the LD50 of S. pullorum ΔsteE was 22.8 times higher than that of S. pullorum in chickens. The colonization experiment of bacteria showed that the overall change trend of the number of S. pullorum and S. pullorum ΔsteE strains were similar in chicken liver, spleen, heart, bursa, and cecum, which increased first and then decreased. However, the deletion of steE caused significantly reduced colonization, pathological change, and virulence of S. pullorum in a chicken infection model. Our findings provide exciting insights into the pathogenic mechanism and live attenuated vaccine associated with steE in S. pullorum.

At present, the current direction in poultry farming is the development of methods for raising poultry without the use of antibiotics to overcome antibiotic resistance. For this purpose, it is recommended to use mineral supplements of chelated forms of microelements of zinc, copper, and manganese in poultry diets. Chelated minerals are characterised by better digestibility during intensive broiler farming, which limits the use of minerals and reduces environmental pollution. The purpose of the study was to examine the quality of meat of broiler chickens of the Cobb-500 cross, provided that chelated forms of zinc, copper, and manganese are included in the diet. Experimental studies were conducted in 2021 on broiler chickens of the Cobb-500 cross. Two groups of 20 heads of poultry were formed to examine the chemical composition of meat. Poultry of the control group received a basic diet with zinc, copper, and manganese sulfates, and poultry of the experimental group – enriched with chelated compounds of these microelements. The addition of chelated compounds of zinc, copper, and manganese to the diet of broiler chickens leads to an increase in the amount of fat, calcium and zinc in white muscles by 69.6, 24.6% and 1.4 times, and in red muscles-by 41.1, 30.9% and 3.4 times, respectively. The content of copper and manganese increases by 48.0 and 95.5% in red muscles and by 28.1 and 15.2% in white muscles compared to the control group. Therewith, there is a decrease in the relative content of essential amino acids by 1.1-1.3% and an increase in non-essential amino acids by 2.6-2.7%. According to the overall assessment of organoleptic parameters of broiler chickens fed zinc, copper, and manganese chelates, the sum of points was 2.0 points higher in the femoral muscles and 1.5 points higher in the pectoral muscles. In addition, according to the tasting assessment of meat from the thigh muscles of broiler chickens, more points were obtained in terms of tenderness by 10.0%, taste by 12.2%, and aroma by 13.2%. According to the tasting assessment of meat from the pectoral muscles of broiler chickens, more points were obtained in terms of tenderness by 18.9% and aroma by 10.3%. According to the reaction with copper sulfate, the content of ammonium and ammonia salts, broiler chicken meat was fresh and obtained from healthy poultry. As a result of organoleptic, physico-chemical, and biochemical studies of broiler chicken meat under the conditions of adding microelement chelates to the diet, it was established that it belongs to fresh and high-quality for consumption. These studies argue for the use of chelated compounds of microelements in poultry farming and contribute to their further introduction into production

Mastitis is an inflammatory disease caused by microbial infection. Chlorogenic acid (CGA), one of the major phenolic acids in Taraxacum officinale, has natural antioxidant and anti-inflammatory properties in various cell types; however, the effects of CGA on Lipoteichoic acid (LTA)-induced bovine mammary epithelial cells (BMECs) have not been investigated. In this study, the CGA content in T. officinale was determined by High-performance liquid chromatography (HPLC). BMECs were infected with LTA to induce the mastitis model. Different concentrations of CGA were administered after establishing the LTA infection. The results showed that the T. officinale contained CGA 1.36 mg/g. CGA significantly reduced the pro-inflammatory gene and protein expression of TNF-α, IL-6, and IL-1β. In addition, CGA downregulated the NO, TLR2, and NF-κB signaling pathways in LTA-infected bovine mammary epithelial cells. Our results indicate that CGA reduced the expression of TNF-α, IL-6, IL-1β, and TLR2 by inhibiting the phosphorylation of proteins in the NF-κB signaling pathways in a dose-dependent manner. This finding suggests that CGA may be a potential agent for the treatment of mastitis in dairy cows.

New S-substituted 4-alkyl-5-((3-(pyridin-4-yl)-1H-1,2,4-triazole-5-yl)thio)methyl)-4H-1,2,4-triazole-3-thiol derivatives have been designed, synthesized and studied their antimicrobial activity on 11 standard Gram-positive and Gram-negative microorganism strains. Their spectral and physicochemical parameters were established using modern comprehensive methods of analysis, including 1H NMR spectroscopy, GC-MS and elemental analysis.It has been found that compound 2a exhibits strong suppression of 5 test strains (MBC = 15.6 µg/mL). Compound 4a showed moderate inhibition of Salmonella pullorum, Escherichia coli O2, Salmonella enteritidis strains (MBC = 31.25 µg/mL) Compound 6a was sensitive toward ten tested bacteria at 31.25 µg/mL concentration.

ABSTRACT: Salmonella is one of the most common Gram-negative pathogens and seriously threatens chicken farms and food safety. This study aimed to establish a multiplex polymerase chain reaction (PCR) approach for the identification of different Salmonella enterica subsp. enterica. The citE2 gene and interval sequence of SPS4_00301–SPS4_00311 existed in all S. enterica subsp. enterica serovars by genomic comparison. By contrast, a 76 bp deletion in citE2 was found only in Salmonella Pullorum. Two pairs of special primers designed from citE2 and interval sequence were used to establish the multiplex PCR system. The optimized multiplex PCR system could distinguish Salmonella Pullorum and non-Salmonella Pullorum. The sensitivity of the optimized multiplex PCR system could be as low as 6.25 pg/μL and 104 colony-forming units (CFU)/mL for genomic DNA and Salmonella Pullorum cells, respectively. The developed multiplex PCR assay distinguished Salmonella Pullorum from 33 different Salmonella enterica subsp. enterica serotypes and 13 non-target species. The detection of egg samples artificially contaminated with Salmonella Pullorum, Salmonella Enteritidis, and naturally contaminated 69 anal swab samples showed that results were consistent with the culture method. These features indicated that the developed multiplex PCR system had high sensitivity and specificity and could be used for the accurate detection of Salmonella Pullorum in clinical samples.

Salmonella enterica serovar Pullorum (S. Pullorum) is a host-specific pathogen, which causes acute gastroenteritis with high mortality in poultry. However, the association between steE, encoded by type III secretion system 2, and Salmonella virulence is not well-understood. To elucidate the functions of steE in S. Pullorum, ΔsteE strain was constructed using the λ-Red recombination technology. Compared to that in the wild-type, the deletion of steE in S. Pullorum reduced bacterial invasion, proliferation, and late apoptosis in the infected HD-11 cells. In addition, we analyzed the mRNA expression levels of effector genes and cytokines by qRT-PCR. SteE was associated with the regulation of various effector genes and inflammatory cytokines in HD-11 cells during S. Pullorum infection. The wild-type effector steE promoted the expression of anti-inflammatory cytokines (IL-4 and IL-10) and reduced that of pro-inflammatory cytokines (IL-1β, IL-6, and IL-12) compared to that in the ΔsteE-infected HD-11 cells and chicken spleens. Results from the chicken infection model showed that the deletion of steE resulted in significantly decreased colonization and long-term survival of the bacteria and alleviated pathological lesions compared to those in the wild-type. Further, steE increased the virulence of S. Pullorum in chickens by regulating the expression of inflammatory cytokines. Our findings provide insights into the persistent infection and autoimmunity associated with steE in S. Pullorum.

Dairy cow mastitis is one of most seriously diseases affecting dairy herds. The prevention and treatment of this pathology is especially done through antimicrobials, but the increasing antimicrobial resistance of pathogens to this disease may affect the efficiency of conventional drugs. Plant extracts are increasingly being valued by livestock producers because of their wide sources, low toxic and side effects, and high environmental affinity. Thereby, a lot of research has been conducted on the control of dairy cow mastitis by plant-derived drugs in recent years. This review summarizes the current of the plant types, main active ingredients, and the mechanism of action of plant extracts for preventing and treating dairy cow mastitis. Finally, a review was carried out to prospect the future development of plant extracts in the treatment of dairy cow mastitis.

The article provides data on the isolation and identification of the pathogen S. Pullorum from pathological material of chickens. With further study of tinctorial, cultural-morphological and biological properties of the pathogen. The susceptibility of Salmonella pullorum to broad-spectrum antibacterial drugs such as cephalosporins and carbopenems was further studied to determine the drug of choice for improving treatment and prevention of avian bacterial diseases. In order to diagnose Salmonella pullorum (S. Pullorum) diarrhea accurately and analyze its drug resistance. In this study, the pathogen of a chicken suspected of S. Pullorum was isolation, PCR amplification and drug sensitivity analysis of the pathogen from in chicken farm in Xinxiang, north China. The results showed that the bacteria strain was diagnosed as S. Pullorum base on isolation and identification, Gram staining and biochemical identification of the bacteria. Antibacterial drugs sensitivity test confirmed that the bacteria was sensitive to ceftiofur, ceftriaxone, meropenem and kanamycin, and the effect of sensitive antibiotics was obvious in clinical treatment. Altogether, the present experiment revealed a detailed measure for S. Pullorum prevention and control and that achieved good clinical results, which laid a fundamental information for farmers and veterinary workers on eradication of S. Pullorum.

Chlorogenic acid is a phenolic compound with antibacterial, antioxidant and antiviral effects. At present, the research on extraction of chlorogenic acid mainly focuses on natural products such as Eucommia ulmoides and honeysuckle. The Taraxacum officinale is more widely distributed and produces. However, there were few studies on extraction technology of chlorogenic acid from Taraxacum officinale. The aim of this research was to investigate detection and extraction process of chlorogenic acid from T. officinale. Taraxacum officinale was used as the materials, and high performance liquid chromatography (HPLC) was used as a reference method to determine the content of chlorogenic acid in the extract solution. And its extraction process was optimized by orthogonal experimental design method. The results showed that the HPLC detection method of chlorogenic acid from Taraxacum officinale was 5% methanol elution condition 0 ~ 5 min., 5 - 15% methanol elution condition 5 ~ 15min, 15 - 5% methanol elution condition 15 ~ 20min., 5 % methanol elution condition 20 ~ 25 min., and the buffer salt was 1‰ phosphoric acid aqueous solution, and the wavelength was 350 nm. The method was stable and reliable. The extraction technology of chlorogenic acid was researched from multiple factors, the best parameters of the technology were obtained that the ultrasonic temperature is 80 ºC, the solid-liquid ratio is 1 : 30, the solvent concentration is 50%, and the ultrasonic time is 40 min., then the extraction rate can reach 1.921%. This study obtained the detection method and optimized the extraction process of chlorogenic acid in Taraxacum officinale, and it provided a research basis for the development and utilization of Taraxacum officinale medicinal value. [ABSTRACT FROM AUTHOR]