1. Semi-Automated Cleanup of Persistent Organic Pollutants in Environmental Samples--Complete Separation of PCDD/Fs and PCBs for Extracts in Toluene.
- Author
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Addink, Ruud and Hall, Tom
- Subjects
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POLLUTANTS , *POLYCHLORINATED dibenzodioxins , *ENVIRONMENTAL sampling , *PERSISTENT pollutants , *TOLUENE , *EXTRACTS , *VACUUM pumps - Abstract
Analysis of polychlorinated dibenzo-p-dioxins (PCDDs), furans (PCDFs), and biphenyls (PCBs) by environmental laboratories in North America has continued for many years. Increasingly analytical needs have focused on complete separation of PCDD/Fs and PCBs during sample processing with all existing 209 PCBs being analyzed. A semi-automated method (EZPrep), which combines elements of fully manual and fully automated procedures, was developed and tested. It uses pre-packaged silica, alumina, and carbon column kits for sample cleanup. The system uses a vacuum pump to elute solvents through these chromatographic columns for sample purification and preparation. The method described here allows for the sample extracts to be in toluene. With this method all PCBs are collected in one fraction and all PCDD/Fs in another fraction. 13C labeled recoveries for 10 g soil extracts were 75 to 95% for PCDD/Fs and 75 to 90% for PCBs. Similar tests for 2 g salmon extracts yielded recoveries of 80 to 105% for PCDD/Fs and 75 to 95% for PCBs. The semi-automated method can process six samples in parallel within a 50 min period. [ABSTRACT FROM AUTHOR]
- Published
- 2020