32,681 results
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2. Sanofi-Cell Research outstanding paper award of 2015.
- Subjects
- Animals, History, 21st Century, Mice, Awards and Prizes, Cell Biology history, Drug Industry economics, Research economics, Research standards
- Published
- 2016
- Full Text
- View/download PDF
3. Sanofi-Cell Research outstanding paper award of 2014.
- Author
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Li D
- Subjects
- Animals, Research Report, Awards and Prizes, Biomedical Research, Cell Biology
- Published
- 2015
- Full Text
- View/download PDF
4. Rock, scissors, paper: How RNA structure informs function
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Sarah M Assmann, Hong-Li Chou, and Philip C Bevilacqua
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Cell Biology ,Plant Science - Abstract
RNA can fold back on itself to adopt a wide range of structures. These range from relatively simple hairpins to intricate 3D folds and can be accompanied by regulatory interactions with both metabolites and macromolecules. The last 50 yr have witnessed elucidation of an astonishing array of RNA structures including transfer RNAs, ribozymes, riboswitches, the ribosome, the spliceosome, and most recently entire RNA structuromes. These advances in RNA structural biology have deepened insight into fundamental biological processes including gene editing, transcription, translation, and structure-based detection and response to temperature and other environmental signals. These discoveries reveal that RNA can be relatively static, like a rock; that it can have catalytic functions of cutting bonds, like scissors; and that it can adopt myriad functional shapes, like paper. We relate these extraordinary discoveries in the biology of RNA structure to the plant way of life. We trace plant-specific discovery of ribozymes and riboswitches, alternative splicing, organellar ribosomes, thermometers, whole-transcriptome structuromes and pan-structuromes, and conclude that plants have a special set of RNA structures that confer unique types of gene regulation. We finish with a consideration of future directions for the RNA structure–function field.
- Published
- 2023
5. Micro-nanofibrillated cellulose preparation from bleached softwood pulp using chemo-refining approach and its evaluation as strength enhancer for paper properties
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Puneet Pathak, Nishi Kant Bhardwaj, and Varun Kumar
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Softwood ,Materials Science (miscellaneous) ,Sodium ,Sodium chlorite ,chemistry.chemical_element ,02 engineering and technology ,engineering.material ,010402 general chemistry ,01 natural sciences ,chemistry.chemical_compound ,Hardwood ,Electrical and Electronic Engineering ,Physical and Theoretical Chemistry ,Fourier transform infrared spectroscopy ,Cellulose ,Pulp (paper) ,Papermaking ,Cell Biology ,021001 nanoscience & nanotechnology ,Pulp and paper industry ,Atomic and Molecular Physics, and Optics ,0104 chemical sciences ,chemistry ,engineering ,0210 nano-technology ,Biotechnology - Abstract
An industry compatible chemo-refining approach was tested for preparation of micro-nanofibrillated cellulose (MNFC) from bleached softwood pulp using sodium meta-periodate and sodium chlorite as oxidizers followed by refining in Valley beater. SEM and FTIR analyses confirmed micro-nano scale fibrillation and chemical functional group modification in laboratory prepared MNFC, respectively. The water retention value, carboxyl content and viscosity of MNFC were found comparable with imported NFC as reference (R-NFC). To evaluate MNFC as strength enhancer for paper properties, 5% MNFC addition to bleached mixed hardwood pulp showed 6% reduction in bulk with 36%, 24% and 97% increment in breaking length, burst index and double fold of the handsheets, respectively without affecting tear index and optical properties than the control. Surface properties were also improved. Pulp drainability (37°SR) after MNFC addition was found suitable for papermaking. These laboratory results confirmed the potential of MNFC as a suitable strength additive for paper quality improvement.
- Published
- 2020
6. Preparation and application of nanocellulose from non-wood plants to improve the quality of paper and cardboard
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O. V. Yashchenko and V. A. Barbash
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Materials science ,Materials Science (miscellaneous) ,Organosolv ,Corrugated fiberboard ,02 engineering and technology ,engineering.material ,010402 general chemistry ,01 natural sciences ,Nanocellulose ,chemistry.chemical_compound ,Lignin ,Electrical and Electronic Engineering ,Physical and Theoretical Chemistry ,biology ,Pulp (paper) ,cardboard ,Cell Biology ,021001 nanoscience & nanotechnology ,biology.organism_classification ,Pulp and paper industry ,Environmentally friendly ,Atomic and Molecular Physics, and Optics ,Kenaf ,0104 chemical sciences ,chemistry ,visual_art ,visual_art.visual_art_medium ,engineering ,0210 nano-technology ,Biotechnology - Abstract
The study describes the preparation of pulp and nanocellulose from non-wood plant materials, as well as an improved properties of paper and cardboard for mass production. The pulps from wheat straw, kenaf and flax fibers were prepared by the environmentally friendly organosolv method—cooking in a solution of isobutanol or peracetic acid. The organosolv pulps used to prepare nanocellulose had traces of lignin and mineral substances. The process of hydrolysis of the investigated organosolv pulps was optimal when carried out under the following conditions: 43% sulfuric acid, temperature 60 °C, hydrolysis time 90 min and ultrasonic treatment 60 min. Using the methods of SEM, XRD, TEM, AFM and TGA, the structure and properties of organosolv pulps and nanocellulose were studied. The use of nanocellulose in bulk and on the surface of mass types of paper and cardboard—paper for corrugation, offset paper, recycled containerboard and cardboard for flat layers of corrugated cardboard were investigated. We established the positive effect of nanocellulose application on the physical and mechanical properties of paper and cardboard. Low consumption of nanocellulose allows production of the paper and cardboard with properties that meet the requirements to appropriate standards and replacement of synthetic reinforcing materials.
- Published
- 2020
7. Facile fabrication of superhydrophobic paper with durability, chemical stability and self-cleaning by roll coating with modified nano-TiO2
- Author
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Yufeng Wang, Yunzhi Chen, Baoying Shi, Yuhong Teng, Ziyan Li, and Weiwei Fan
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Materials science ,Materials Science (miscellaneous) ,02 engineering and technology ,engineering.material ,010402 general chemistry ,01 natural sciences ,Contact angle ,Coating ,Electrical and Electronic Engineering ,Physical and Theoretical Chemistry ,Composite material ,Sandpaper ,Filter paper ,Cell Biology ,Epoxy ,021001 nanoscience & nanotechnology ,Atomic and Molecular Physics, and Optics ,Surface energy ,0104 chemical sciences ,visual_art ,engineering ,visual_art.visual_art_medium ,Adhesive ,0210 nano-technology ,Layer (electronics) ,Biotechnology - Abstract
A superhydrophobic paper with excellent robustness was fabricated by roll coating with modified nano-TiO2. First, nano-TiO2 particles were hydrophobically modified by γ-aminopropyltriethoxysilane and 1H, 1H, 2H, 2H-perfluorooctyltriethoxysilane (POTS). Then the paper coating composed of modified nano-TiO2 particles as pigments and epoxy resin (EP) as adhesives was coated on a paper surface to create the desired surface morphology and surface energy. Compared with the uncoated filter paper, the prepared paper showed an improved rough surface morphology owing to the uniform layer of TiO2 microclusters deposited on the fiber network. The superhydrophobic paper exhibited water contact angles (WCA) about 153° ± 1.5°, and water sliding angles (WSA) about 3.5° ± 0.5°, low surface adhesion and excellent bounce ability. The superhydrophobic paper can withstand a variety of wear and tear, such as tape stripping and knife scraping. The as-prepared superhydrophobic paper showed mechanical durability even after 20 wear cycles with sandpaper,thus sustaining excellent superhydrophobicity on the filter paper surface. Moreover, it remains fully functional even in environments with high concentrations of acid and alkali for 96 h. The superhydrophobicity was not affected after storage for 6 months in a natural environment. It was confirmed that the superhydrophobic paper surface exhibited excellent chemical stability, long-term stability and self-cleaning properties. The entire production process was operated under normal environment, without complex equipment; and, as such, has the possibility for large-scale production and application in industry.
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- 2020
8. Engagement in PC-based, smartphone-based, and paper-based materials: Learning vocabulary through Chinese Stories
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Yijen Wang
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Embryology ,Vocabulary ,Multimedia ,Computer science ,media_common.quotation_subject ,Cell Biology ,Paper based ,Anatomy ,computer.software_genre ,computer ,Developmental Biology ,media_common - Published
- 2020
9. Call for Papers: 'Targeting Airway Immunity in Lung Disease'
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Nathan W. Bartlett, Carol Feghali-Bostwick, and Susan J. Gunst
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Pulmonary and Respiratory Medicine ,Physiology ,Physiology (medical) ,Cell Biology - Published
- 2023
10. Urinary Extracellular Vesicles: A Position Paper by the Urine Task Force of the International Society for Extracellular Vesicles
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Juan M. Falcón-Pérez, Dylan Burger, Aled Clayton, Lei Zheng, Uta Erdbrügger, Kerstin Junker, Luca Musante, Ewout J. Hoorn, I.V. Bijnsdorp, Kenneth W. Witwer, Harry Holthöfer, James W. Dear, Erik H. Koritzinsky, Benedetta Bussolati, Jason P. Webber, Elena S. Martens-Uzunova, Charles J. Blijdorp, Inge Mertens, Alicia Llorente, James M. Luther, Peter S.T. Yuen, Catherine Sánchez, Janne Leivo, Andrew F. Hill, Guido Jenster, Eline Oeyen, Visith Thongboonkerd, Cristina Grange, Metka Lenassi, Maija Puhka, Carolina Soekmadji, Volkert van Steijn, Francesc E. Borràs, James Brian Byrd, Martin E. van Royen, Gerald W. Verhaegh, Mark A. Knepper, John Klein, Connie R. Jimenez, Institute for Molecular Medicine Finland, University of Helsinki, Internal Medicine, Urology, Pathology, CCA - Cancer biology and immunology, CCA - Imaging and biomarkers, Medical oncology laboratory, and Amsterdam Neuroscience - Neurodegeneration
- Subjects
0301 basic medicine ,CLINICAL-APPLICATIONS ,Urine ,0302 clinical medicine ,Medicine ,Biomarker discovery ,Urinary Tract ,bladder ,biobank ,biomarkers ,extracellular vesicles ,kidney ,liquid biopsy ,prostate ,rigor and standardization ,urine ,DEEP SEQUENCING ANALYSIS ,Prostate ,TAMM-HORSFALL PROTEIN ,Reference Standards ,Extracellular vesicles ,BIOMARKER DISCOVERY ,Body Fluids ,3. Good health ,PROSTATE-CANCER ,Clinical Practice ,030220 oncology & carcinogenesis ,Position Paper ,PROTEOMIC ANALYSIS ,MESSENGER-RNA ,MEMBRANE-VESICLES ,Histology ,Urinary system ,Advisory Committees ,Scientific field ,Bladders ,DIABETIC-NEPHROPATHY ,03 medical and health sciences ,All institutes and research themes of the Radboud University Medical Center ,Urological cancers Radboud Institute for Molecular Life Sciences [Radboudumc 15] ,Humans ,Biology ,QH573-671 ,Task force ,business.industry ,Reproducibility of Results ,Kidneys ,Cell Biology ,Biobanks ,SODIUM-CHLORIDE COTRANSPORTER ,030104 developmental biology ,Clinical diagnosis ,Position paper ,1182 Biochemistry, cell and molecular biology ,Human medicine ,Societies ,business ,Cytology ,Position Papers ,Neuroscience ,Biomarkers - Abstract
Urine is commonly used for clinical diagnosis and biomedical research. The discovery of extracellular vesicles (EV) in urine opened a new fast-growing scientific field. In the last decade urinary extracellular vesicles (uEVs) were shown to mirror molecular processes as well as physiological and pathological conditions in kidney, urothelial and prostate tissue. Therefore, several methods to isolate and characterize uEVs have been developed. However, methodological aspects of EV separation and analysis, including normalization of results, need further optimization and standardization to foster scientific advances in uEV research and a subsequent successful translation into clinical practice. This position paper is written by the Urine Task Force of the Rigor and Standardization Subcommittee of ISEV consisting of nephrologists, urologists, cardiologists and biologists with active experience in uEV research. Our aim is to present the state of the art and identify challenges and gaps in current uEV-based analyses for clinical applications. Finally, recommendations for improved rigor, reproducibility and interoperability in uEV research are provided in order to facilitate advances in the field. ESM-U, CG, GV, GJ, IVB, MvR, and VvS, are members of the “IMMPROVE” consortium (Innovative Measurements and Markers for Prostate Cancer Diagnosis and Prognosis using Extracellular Vesicles), which is sponsored by an Alpe d'HuZes grant of the Dutch Cancer Society (grant #EMCR2015-8022). AL is supported by Norges Forskningsråd, Kreftforeningen and Helse Sør-Øst RHF (NO). UE is supported by the NIH, National Heart, Lung, and Blood Institute, Award number K23-HL-126101. CJB and EJH are supported by the Dutch Kidney Foundation (Nierstichting), Award number: CP18.05.
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- 2021
11. Call for Papers: 'Novel Insights into Preterm Respiratory Physiology: Celebrating the 100th Birthday of Dr. Mildred T. Stahlman'
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Rory Morty and Sue Bodine
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Pulmonary and Respiratory Medicine ,Physiology ,Physiology (medical) ,Cell Biology - Published
- 2022
12. Considerations for immune effector cell therapy collections: a white paper from the American Society for Apheresis
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Hien D. Liu, Leon Su, Jeffrey L. Winters, Suzanne R. Thibodeaux, Yara A. Park, YanYun Wu, Joseph Schwartz, Abba C. Zubair, Jennifer Schneiderman, Gaurav K. Gupta, Sharanya Ramakrishnan, and Nicole A. Aqui
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Adult ,Cancer Research ,Transplantation ,Consensus ,Immunology ,Cell- and Tissue-Based Therapy ,Cell Biology ,Tissue Donors ,United States ,Oncology ,Blood Component Removal ,Humans ,Immunology and Allergy ,Leukapheresis ,Child ,Genetics (clinical) - Abstract
This white paper was developed to provide leukapheresis guidance for the collection of mononuclear cells from adult and pediatric patients who are destined for immune effector cell (IEC) therapies for commercial and research applications. Currently, there is considerable variability in leukapheresis processes and limited published information regarding best practices relevant to new cellular therapies, especially IECs. Herein the authors address critical leukapheresis questions in five domains to help guide consistent collection processes and ensure high-quality products. The first four domains are onboarding, pre-collection, collection and post-collection, with protocol feasibility, preparation, care and follow-up of the patient/donor at each step, respectively, and technical considerations during collection. The fifth domain of quality assurance focuses on ensuring product potency, purity, safety and auditing.The American Society for Apheresis (ASFA) Clinical Applications Committee (IEC Therapy Subcommittee) was charged by the society's board of directors with working collaboratively with other ASFA committees and organizations, including the Foundation for the Accreditation of Cellular Therapy, Association for the Advancement of Blood and Biotherapies, American Society for Transplantation and Cellular Therapy, National Marrow Donor Program and International Society for CellGene Therapy, to develop guidelines regarding leukapheresis collection of cells destined for the manufacture of IEC therapies. After a review of the literature and discussion with members of the involved committees and various institutions, a draft guidance was created and circulated for comment and revision.Critical aspects of apheresis that could affect the quality and quantity of the leukapheresis product were identified. These areas were then discussed and reviewed. After consensus, the best practice guidelines were proposed and accepted.In the current era of rapid growth of IEC therapies, it is important to address critical leukapheresis steps to provide high-quality products and more consistent practices and to eliminate redundant efforts.
- Published
- 2022
13. Fluorescent Immunological Paper-based Assay for Exosome detection
- Author
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Surasak Kasetsirikul, Muhammad J. A. Shiddiky, and Nam-Trung Nguyen
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Chemistry ,Paper based ,Fluorescence ,Exosome ,Cell biology - Abstract
This paper reports the development of fluorescent-linked immunosorbent paper-based assay for exosome detection. The paper-based device was fabricated with sandwich lamination for easy handling and was coated with exosome-specific antibody as a biosensing platform to detect exosome sample from the cell culture media. This assay employed fluorescent detection which is followed by tagging fluorophore-conjugated detecting antibody on exosome samples. The fluorescent readout was evaluated and quantified from image processing software. This assay can detect high concentration of exosome samples (~ 1010 exosome/mL). However, this assay has encountered various challenges. First, the exosome concentration prepared from cell culture media from cancer-derived ovarian and mesothelial cell lines may be insufficient to reach detectable range. Second, chemical contamination from exosome isolation kits may affect assay sensitivity. Therefore, assay optimization and minimizing chemical contamination are required which could enhance assay specificity and sensitivity.
- Published
- 2021
14. Paper-based ELISA diagnosis technology for human brucellosis based on a multiepitope fusion protein
- Author
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Han Li, Hai Jiang, Mingjun Sun, Qiongqiong Bai, Dehui Yin, Jingpeng Zhang, Xiling Wu, and Jihong Shao
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Bacterial Diseases ,Serum Proteins ,B Cells ,RC955-962 ,Disease ,Pathology and Laboratory Medicine ,Biochemistry ,Epitope ,Cell Fusion ,Epitopes ,White Blood Cells ,Medical Conditions ,Filter Paper ,Animal Cells ,Zoonoses ,Arctic medicine. Tropical medicine ,Medicine and Health Sciences ,Enzyme-Linked Immunoassays ,biology ,Bacterial Pathogens ,Laboratory Equipment ,medicine.anatomical_structure ,Infectious Diseases ,Medical Microbiology ,Engineering and Technology ,Pathogens ,Cellular Types ,Public aspects of medicine ,RA1-1270 ,Bacterial Outer Membrane Proteins ,Research Article ,Neglected Tropical Diseases ,China ,Cell Physiology ,Immune Cells ,Immunology ,Equipment ,Enzyme-Linked Immunosorbent Assay ,Brucella ,Research and Analysis Methods ,Sensitivity and Specificity ,Microbiology ,Brucellosis ,Antigen ,Diagnostic Medicine ,medicine ,Humans ,Immunoassays ,Antibody-Producing Cells ,Microbial Pathogens ,B cell ,Antigens, Bacterial ,Blood Cells ,Bacteria ,business.industry ,Public Health, Environmental and Occupational Health ,Organisms ,Biology and Life Sciences ,Proteins ,Cell Biology ,medicine.disease ,biology.organism_classification ,Tropical Diseases ,Fusion protein ,Virology ,Infectious disease (medical specialty) ,Immunologic Techniques ,business - Abstract
Background Brucellosis, as a serious zoonotic infectious disease, has been recognized as a re-emerging disease in the developing countries worldwide. In china, the incidence of brucellosis is increasing each year, seriously threatening the health of humans as well as animal populations. Despite a quite number of diagnostic methods currently being used for brucellosis, innovative technologies are still needed for its rapid and accurate diagnosis, especially in area where traditional diagnostic is unavailable. Methodology/Principal findings In this study, a total of 22 B cell linear epitopes were predicted from five Brucella outer membrane proteins (OMPs) using an immunoinformatic approach. These epitopes were then chemically synthesized, and with the method of indirect ELISA (iELISA), each of them displayed a certain degree of capability in identifying human brucellosis positive sera. Subsequently, a fusion protein consisting of the 22 predicted epitopes was prokaryotically expressed and used as diagnostic antigen in a newly established brucellosis testing method, nano-ZnO modified paper-based ELISA (nano-p-ELISA). According to the verifying test using a collection of sera collected from brucellosis and non-brucellosis patients, the sensitivity and specificity of multiepitope based nano-p-ELISA were 92.38% and 98.35% respectively. The positive predictive value was 98.26% and the negative predictive value was 91.67%. The multiepitope based fusion protein also displayed significantly higher specificity than Brucella lipopolysaccharide (LPS) antigen. Conclusions B cell epitopes are important candidates for serologically testing brucellosis. Multiepitope fusion protein based nano-p-ELISA displayed significantly sensitivity and specificity compared to Brucella LPS antigen. The strategy applied in this study will be helpful to develop rapid and accurate diagnostic method for brucellosis in human as well as animal populations., Author summary Brucellosis is one of the most important zoonosis in the world and has caused tremendous economic losses in agriculture and animal husbandry in many countries. Developing rapid, sensitive and specific diagnostic methods is very important for early detection and treatment of brucellosis patients. In this study, a novel diagnostic technique, nano-ZnO modified paper ELISA, was established. The antigen used in this technique was a fusion protein containing multiple B cell epitopes, which were predicted from Brucella major outer membrane proteins such as Bp26, Omp31, Omp16, Omp2b and Omp25. Comparing to traditional LPS antigen, this multiepitope based antigen displayed considerably higher sensitivity and higher specificity in laboratory. With the strategy described in this paper, more efficient epitopes and protein antigen can be identified in the future. Currently, LPS antigen is only prepared from live Brucella, while protein antigen can be produced in large quantities in prokaryotic expression system. In addition to nano-p-ELISA, this protein antigen can also be used for development other methods such as fluorescent polarization assay (FPA) and immunochromatographic assay (ICA) to meet the varied demand for brucellosis testing.
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- 2021
15. Survival of SARS-COV-2 under liquid medium, dry filter paper and acidic conditions
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Zhiping Sun, Xia Cai, Wendong Han, Yang Wu, Chenjian Gu, Rong Zhang, Youhua Xie, Zhenghong Yuan, Di Qu, Wei Xu, Xunjia Cheng, Yuyan Wang, and Yun Qian
- Subjects
2019-20 coronavirus outbreak ,Coronavirus disease 2019 (COVID-19) ,Filter paper ,business.industry ,lcsh:Cytology ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,Cell Biology ,Liquid medium ,Biochemistry ,Virology ,Correspondence ,Genetics ,Medicine ,lcsh:QH573-671 ,business ,Molecular Biology - Published
- 2020
16. Paper-based Transwell assays: an inexpensive alternative to study cellular invasion
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Adam Loeser, Nathan A. Whitman, Matthew R. Lockett, and Rachael M. Kenney
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Paper ,02 engineering and technology ,01 natural sciences ,Biochemistry ,Article ,Analytical Chemistry ,Extracellular matrix ,Cell Movement ,Cell Line, Tumor ,Electrochemistry ,Animals ,Humans ,Environmental Chemistry ,Neoplasm Invasiveness ,Spectroscopy ,Chemistry ,010401 analytical chemistry ,Exogenous factor ,Disease progression ,Reproducibility of Results ,Cell movement ,Paper based ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Cell biology ,Cell culture ,Biological Assay ,Cattle ,0210 nano-technology - Abstract
Cellular movement is essential in the formation and maintenance of healthy tissues as well as in disease progression such as tumor metastasis. In this work, we describe a paper-based Transwell assay capable of quantifying cellular invasion through an extracellular matrix. The paper-based Transwell assays generate similar datasets, with equivalent reproducibility, to commercially available Transwell assays. With different culture configurations, we quantify invasion: upon addition of an exogenous factor or in the presence of medium obtained from other cell types, in an indirect or direct co-culture format whose medium composition is dynamically changing, and in a single-zone or parallel (96-zone) format.
- Published
- 2019
17. Growing a circular economy with fungal biotechnology: a white paper
- Author
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Meyer, Vera, Basenko, Evelina Y, Benz, J Philipp, Braus, Gerhard H, Caddick, Mark X, Csukai, Michael, de Vries, Ronald P, Endy, Drew, Frisvad, Jens C, Gunde-Cimerman, Nina, Haarmann, Thomas, Hadar, Yitzhak, Hansen, Kim, Johnson, Robert I, Keller, Nancy P, Kraševec, Nada, Mortensen, Uffe H, Perez, Rolando, Ram, Arthur F J, Record, Eric, Ross, Phil, Shapaval, Volha, Steiniger, Charlotte, van den Brink, Hans, van Munster, Jolanda, Yarden, Oded, Wösten, Han A B, Sub Molecular Plant Physiology, Sub Molecular Microbiology, Molecular Microbiology, Molecular Plant Physiology, Department of Molecular Microbiology and Biotechnology, Leiden University, Institute of Biology Leiden, Georg-August-University [Göttingen], School of Biological Sciences, University of Liverpool, Science Faculty, Mohamed V University in Rabat, Chercheur indépendant, Technical University of Denmark [Lyngby] (DTU), Centre of Excellence for Integrated Approaches in Chemistry and Biology of Proteins, University of Virginia [Charlottesville], Centre of Molecular Immunology, Antibody engineering Department, Leiden University, Unité de microbiologie et technologie céréalières, Institut National de la Recherche Agronomique (INRA), Norwegian University of Life Sciences (NMBU), Department of Plant Pathology and Microbiology, The Hebrew University of Jerusalem (HUJ), Utrecht University [Utrecht], Sub Molecular Plant Physiology, Sub Molecular Microbiology, Molecular Microbiology, Molecular Plant Physiology, Westerdijk Fungal Biodiversity Institute - Fungal Physiology, and Westerdijk Fungal Biodiversity Institute
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0106 biological sciences ,[SDV.BIO]Life Sciences [q-bio]/Biotechnology ,lcsh:Biotechnology ,[SDV]Life Sciences [q-bio] ,Population ,Automotive industry ,Review ,01 natural sciences ,Applied Microbiology and Biotechnology ,12. Responsible consumption ,03 medical and health sciences ,White paper ,lcsh:TP248.13-248.65 ,010608 biotechnology ,11. Sustainability ,SDG 13 - Climate Action ,education ,Molecular Biology ,Ecology, Evolution, Behavior and Systematics ,ComputingMilieux_MISCELLANEOUS ,030304 developmental biology ,2. Zero hunger ,Sustainable development ,0303 health sciences ,education.field_of_study ,business.industry ,Circular economy ,SDG 8 - Decent Work and Economic Growth ,Cell Biology ,[SDE.ES]Environmental Sciences/Environmental and Society ,Biotechnology ,Climate change mitigation ,13. Climate action ,Greenhouse gas ,Sustainability ,Business ,SDG 12 - Responsible Consumption and Production - Abstract
Fungi have the ability to transform organic materials into a rich and diverse set of useful products and provide distinct opportunities for tackling the urgent challenges before all humans. Fungal biotechnology can advance the transition from our petroleum-based economy into a bio-based circular economy and has the ability to sustainably produce resilient sources of food, feed, chemicals, fuels, textiles, and materials for construction, automotive and transportation industries, for furniture and beyond. Fungal biotechnology offers solutions for securing, stabilizing and enhancing the food supply for a growing human population, while simultaneously lowering greenhouse gas emissions. Fungal biotechnology has, thus, the potential to make a significant contribution to climate change mitigation and meeting the United Nation’s sustainable development goals through the rational improvement of new and established fungal cell factories. The White Paper presented here is the result of the 2nd Think Tank meeting held by the EUROFUNG consortium in Berlin in October 2019. This paper highlights discussions on current opportunities and research challenges in fungal biotechnology and aims to inform scientists, educators, the general public, industrial stakeholders and policymakers about the current fungal biotech revolution.
- Published
- 2020
18. ESC Working Group on Cellular Biology of the Heart: position paper for Cardiovascular Research: tissue engineering strategies combined with cell therapies for cardiac repair in ischaemic heart disease and heart failure
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Fabrice Prunier, Péter Ferdinandy, Joost P.G. Sluijter, Sandrine Lecour, Sophie Van Linthout, Linda W. van Laake, Maurizio Pesce, Hans Erik Bøtker, Kirsti Ytrehus, Sean M. Davidson, Felix B. Engel, Rosalinda Madonna, Francesco Fernandez-Aviles, Raffaele De Caterina, Cinzia Perrino, Philippe Menasché, Derek J. Hausenloy, Thomas Eschenhagen, Wolfram-Hubertus Zimmermann, Jonathan Leor, Jean-Sébastien Hulot, and Perrino, C
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0301 basic medicine ,Biomedical Research ,Consensus ,Physiology ,Cells ,Cell ,Cardiology ,Myocardial Ischemia ,Cardiac tissue engineering ,030204 cardiovascular system & hematology ,Biomaterials ,Cell therapy ,03 medical and health sciences ,0302 clinical medicine ,Tissue engineering ,Physiology (medical) ,medicine ,Humans ,Regeneration ,Survival rate ,Heart Failure ,Heart failure ,Ischaemic heart disease ,Tissue Engineering ,business.industry ,Myocardium ,Regeneration (biology) ,Position Paper from European Society of Cardiology Working Group ,Recovery of Function ,medicine.disease ,Cell biology ,Transplantation ,Treatment Outcome ,030104 developmental biology ,medicine.anatomical_structure ,Position paper ,Cardiology and Cardiovascular Medicine ,business ,Stem Cell Transplantation - Abstract
Morbidity and mortality from ischaemic heart disease (IHD) and heart failure (HF) remain significant in Europe and are increasing worldwide. Patients with IHD or HF might benefit from novel therapeutic strategies, such as cell-based therapies. We recently discussed the therapeutic potential of cell-based therapies and provided recommendations on how to improve the therapeutic translation of these novel strategies for effective cardiac regeneration and repair. Despite major advances in optimizing these strategies with respect to cell source and delivery method, the clinical outcome of cell-based therapy remains unsatisfactory. Major obstacles are the low engraftment and survival rate of transplanted cells in the harmful microenvironment of the host tissue, and the paucity or even lack of endogenous cells with repair capacity. Therefore, new ways of delivering cells and their derivatives are required in order to empower cell-based cardiac repair and regeneration in patients with IHD or HF. Strategies using tissue engineering (TE) combine cells with matrix materials to enhance cell retention or cell delivery in the transplanted area, and have recently received much attention for this purpose. Here, we summarize knowledge on novel approaches emerging from the TE scenario. In particular, we will discuss how combinations of cell/bio-materials (e.g. hydrogels, cell sheets, prefabricated matrices, microspheres, and injectable matrices) combinations might enhance cell retention or cell delivery in the transplantation areas, thereby increase the success rate of cell therapies for IHD and HF. We will not focus on the use of classical engineering approaches, employing fully synthetic materials, because of their unsatisfactory material properties which render them not clinically applicable. The overall aim of this Position Paper from the ESC Working Group Cellular Biology of the Heart is to provide recommendations on how to proceed in research with these novel TE strategies combined with cell-based therapies to boost cardiac repair in the clinical settings of IHD and HF. © Published on behalf of the European Society of Cardiology. All rights reserved.
- Published
- 2019
19. Paper-based plasmonic substrates as surface-enhanced Raman scattering spectroscopy platforms for cell culture applications
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L. Guerrini, Yanan Kang, K. Juarez-Moreno, José M. Romo-Herrera, R.A. Alvarez-Puebla, Wolfgang J. Parak, N. Feliu, and Publica
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Medicine (General) ,Materials science ,Biocompatibility ,QH301-705.5 ,Biomedical Engineering ,Nanoparticle ,Bioengineering ,Nanotechnology ,Review Article ,Nanomaterials ,Biomaterials ,R5-920 ,Biology (General) ,Plasmonic nanoparticles ,Molecular Biology ,Plasmon ,SERS ,Paper-based substrates ,Cell Biology ,Plasmonic papers ,Colloidal gold ,Cell culture ,Biosensor ,Biotechnology - Abstract
The engineering of advanced materials capable of mimicking the cellular micro-environment while providing cells with physicochemical cues is central for cell culture applications. In this regard, paper meets key requirements in terms of biocompatibility, hydrophilicity, porosity, mechanical strength, ease of physicochemical modifications, cost, and ease of large-scale production, to be used as a scaffold material for biomedical applications. Most notably, paper has demonstrated the potential to become an attractive alternative to conventional biomaterials for creating two-dimensional (2D) and three-dimensional (3D) biomimetic cell culture models that mimic the features of in vivo tissue environments for improving our understanding of cell behavior (e.g. growth, cell migration, proliferation, differentiation and tumor metastasis) in their natural state. On the other hand, integration of plasmonic nanomaterials (e.g. gold nanoparticles) within the fibrous structure of paper opens the possibility to generate multifunctional scaffolds equipped with biosensing tools for monitoring different cell cues through physicochemical signals. Among different plasmonic based detection techniques, surface-enhanced Raman scattering (SERS) spectroscopy emerged as a highly specific and sensitive optical tool for its extraordinary sensitivity and the ability for multidimensional and accurate molecular identification. Thus, paper-based plasmonic substrates in combination with SERS optical detection represent a powerful future platform for monitoring cell cues during cell culture processes. To this end, in this review, we will describe the different methods for fabricating hybrid paper-plasmonic nanoparticle substrates and their use in combination with SERS spectroscopy for biosensing and, more specifically, in cell culture applications., Graphical abstract Image 1
- Published
- 2021
20. Bibliometric analysis of scientific papers on extracellular vesicles in kidney disease published between 1999 and 2022
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Marady Hun, Huai Wen, Phanna Han, Tharith Vun, Mingyi Zhao, and Qingnan He
- Subjects
Cell Biology ,Developmental Biology - Abstract
Background: In recent years, there has been an increasing interest in using extracellular vesicles (EVs) as potential therapeutic agents or natural drug delivery systems in kidney-related diseases. However, a detailed and targeted report on the current condition of extracellular vesicle research in kidney-related diseases is lacking. Therefore, this prospective study was designed to investigate the use of bibliometric analysis to comprehensively overview the current state of research and frontier trends on extracellular vesicle research in kidney-related diseases using visualization tools.Methods: The Web of Science Core Collection (WoSCC) database was searched to identify publications related to extracellular vesicle research in kidney-related diseases since 1999. Citespace, Microsoft Excel 2019, VOSviewer software, the R Bibliometrix Package, and an online platform were used to analyze related research trends to stratify the publication data and collaborations.Results: From 1 January 1999 to 26 June 2022, a total of 1,122 EV-related articles and reviews were published, and 6,486 authors from 1,432 institutions in 63 countries or regions investigated the role of extracellular vesicles in kidney-related diseases. We found that the number of articles on extracellular vesicles in kidney-related diseases increased every year. Dozens of publications were from China and the United States. China had the most number of related publications, in which the Southeast University (China) was the most active institution in all EV-related fields. Liu Bi-cheng published the most papers on extracellular vesicles, while Clotilde Théry had the most number of co-citations. Most papers were published by The International Journal of Molecular Sciences, while Kidney International was the most co-cited journal for extracellular vesicles. We found that exosome-related keywords included exosome, exosm, expression, extracellular vesicle, microRNA, microvesicle, and liquid biopsy, while disease- and pathological-related keywords included biomarker, microRNA, apoptosis, mechanism, systemic lupus erythematosus, EGFR, acute kidney injury, and chronic kidney disease. Acute kidney disease (AKI), CKD, SLE, exosome, liquid biopsy, and extracellular vesicle were the hotspot in extracellular vesicle and kidney-related diseases research.Conclusion: The field of extracellular vesicles in kidney-related disease research is rapidly growing, and its domain is likely to expand in the next decade. The findings from this comprehensive analysis of extracellular vesicles in kidney-related disease research could help investigators to set new diagnostic, therapeutic, and prognostic ideas or methods in kidney-related diseases.
- Published
- 2023
21. Call for papers: cyclical function of the female reproductive tract
- Author
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Michele Boiani and Francesca E Duncan
- Subjects
Embryology ,Reproductive Medicine ,Genetics ,Obstetrics and Gynecology ,Cell Biology ,Molecular Biology ,Developmental Biology - Published
- 2023
22. LSA-50 paper: An alternative to P81 phosphocellulose paper for radiometric protein kinase assays
- Author
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Rudra Kashyap, Johan Van Lint, Olivia Appelmans, Arnout Voet, Wim M. De Borggraeve, and Philippe Gilles
- Subjects
Paper ,Biochemistry & Molecular Biology ,Cation exchange paper ,Biophysics ,Biochemistry ,Biochemical Research Methods ,Protein kinase ,Research community ,Alternative paper ,Luciferase ,Kinase activity ,Cellulose ,Radiometry ,Protein kinase A ,Molecular Biology ,Science & Technology ,Kinase ,Chemistry ,Chemistry, Analytical ,Cell Biology ,Radiometric kinase assay ,Physical Sciences ,Phosphocellulose paper ,Life Sciences & Biomedicine ,Protein Kinases - Abstract
Radiometric assays have widely been used for measuring protein kinase activity for decades. In addition, several non-radiometric kinase assay formats have been developed over the years, including luciferase-based and fluorescence-based assays. However, radiometric assays are still considered as the "gold standard" for protein kinase assays, because of their direct readout, high sensitivity, reproducibility, reliability, and very low background signals. These radiometric assays rely on P81 phosphocellulose paper to capture the phosphorylated substrate and wash out unreacted [γ-32P] ATP. However, recently the production of P81 was discontinued by the manufacturer, causing major concern within the protein kinase research community. The advantages of radiometric assays over other kinase assay methods call for an urgent alternative to the discontinued P81 paper. In this report, we demonstrate that the LSA-50 paper is a worthy alternative for radiometric protein kinase assays originally using P81 phosphocellulose paper. ispartof: ANALYTICAL BIOCHEMISTRY vol:630 ispartof: location:United States status: published
- Published
- 2021
23. Design and fabrication of microfibrous composite scaffold by coating clindamycin and chitosan onto cellulose filter paper for wound dressing applications
- Author
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Sajjad Haider, Nadia Farooq, Rawaiz khan, Syed Babar Jamal, Dalal alotaibi, Bushra Bano, Nargis Jamila, Muhammad Naeem, Ali alrahlah, Muhammad Umar Aslam Khan, Adnan Haider, and Naeem Khan
- Subjects
Materials Science (miscellaneous) ,Cell Biology ,Electrical and Electronic Engineering ,Physical and Theoretical Chemistry ,Atomic and Molecular Physics, and Optics ,Biotechnology - Published
- 2022
24. Paper-based in vitro tissue chip for delivering programmed mechanical stimuli of local compression and shear flow
- Author
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Marianne Madias, Patarajarin Akarapipad, Jeong Yeol Yoon, Kattika Kaarj, and Soohee Cho
- Subjects
0301 basic medicine ,Microcontroller ,Environmental Engineering ,Materials science ,Paper-based cell culture ,Biomedical Engineering ,02 engineering and technology ,Bending ,Servomotor ,03 medical and health sciences ,Automated flow control ,Tissue Chip ,Cell migration ,lcsh:QH301-705.5 ,Molecular Biology ,ComputingMethodologies_COMPUTERGRAPHICS ,Methodology ,Cell Biology ,Vascular endothelial cell ,021001 nanoscience & nanotechnology ,Compression (physics) ,Chip ,Shear (sheet metal) ,030104 developmental biology ,lcsh:Biology (General) ,0210 nano-technology ,Shear flow ,Biomedical engineering - Abstract
Abstract Mechanical stimuli play important roles on the growth, development, and behavior of tissue. A simple and novel paper-based in vitro tissue chip was developed that can deliver two types of mechanical stimuli—local compression and shear flow—in a programmed manner. Rat vascular endothelial cells (RVECs) were patterned on collagen-coated nitrocellulose paper to create a tissue chip. Localized compression and shear flow were introduced by simply tapping and bending the paper chip in a programmed manner, utilizing an inexpensive servo motor controlled by an Arduino microcontroller and powered by batteries. All electrical compartments and a paper-based tissue chip were enclosed in a single 3D-printed enclosure, allowing the whole device to be independently placed within an incubator. This simple device effectively simulated in vivo conditions and induced successful RVEC migration in as early as 5 h. The developed device provides an inexpensive and flexible alternative for delivering mechanical stimuli to other in vitro tissue models. Graphical abstract
- Published
- 2020
25. The raw truth about paper mills
- Author
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Jana Christopher
- Subjects
Publishing ,0303 health sciences ,Biomedical Research ,Computer science ,030302 biochemistry & molecular biology ,Biophysics ,ComputerApplications_COMPUTERSINOTHERSYSTEMS ,Cell Biology ,Biochemistry ,Data science ,03 medical and health sciences ,Structural Biology ,Genetics ,Humans ,Periodicals as Topic ,Raw data ,Molecular Biology ,Relevant information ,030304 developmental biology - Abstract
In 2018, the community first observed scientific papers in the biomedical literature that seemed to display systematically fabricated data, pointing to the existence of paper mills: unofficial, potentially illegal organizations selling fake scientific manuscripts. In the present article, we share relevant information specifically about the 'raw data' associated with paper mill manuscripts. If a submitted manuscript displays clear indicators of potential paper mill involvement, we found that the raw data at close inspection often raise doubts about their authenticity. In the absence of real data, paper mills may need to fabricate raw data images when responding to requests from journals. Given the necessity to streamline production of fake manuscripts, the alleged raw data might be created using templates. Some potential methods for generating fake Western blot images are discussed. Paying close attention to image data, including graphs, diagrams, plots and tables, ideally at pre-publication stage, can clearly help prevent publication of incorrect and fabricated information.
- Published
- 2021
26. Call for Papers: 'In It for the Long Haul: Understanding the Lasting Impact of COVID-19 on Lung Health and Disease'
- Author
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Nathan W. Bartlett, Julie A. Bastarache, Wolfgang M. Kuebler, and Eric P. Schmidt
- Subjects
Pulmonary and Respiratory Medicine ,Time Factors ,Physiology ,Physiology (medical) ,Humans ,COVID-19 ,Cell Biology ,Lung - Published
- 2022
27. MS-based lipidomics of human blood plasma: a community-initiated position paper to develop accepted guidelines
- Author
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Xianlin Han, Tze Ping Loh, Bo Burla, Michelle K. Lin, Makoto Arita, Masanori Arita, Edward A. Dennis, Kim Ekroos, Anne K. Bendt, Michael J.O. Wakelam, Amaury Cazenave-Gassiot, Federico Torta, Oswald Quehenberger, Markus R. Wenk, Kazutaka Ikeda, Craig E. Wheelock, Andrej Shevchenko, Matej Orešič, Peter J. Meikle, and Gerhard Liebisch
- Subjects
0301 basic medicine ,Sample handling ,Human blood ,business.industry ,ta1182 ,Cell Biology ,Computational biology ,Shotgun lipidomics ,Lipidome ,Biochemistry ,Biological fluid ,3. Good health ,03 medical and health sciences ,030104 developmental biology ,Endocrinology ,Lipidomics ,Blood plasma ,Position paper ,Medicine ,business - Abstract
Human blood is a self-regenerating lipid-rich biological fluid that is routinely collected in hospital settings. The inventory of lipid molecules found in blood plasma (plasma lipidome) offers insights into individual metabolism and physiology in health and disease. Disturbances in the plasma lipidome also occur in conditions that are not directly linked to lipid metabolism; therefore, plasma lipidomics based on MS is an emerging tool in an array of clinical diagnostics and disease management. However, challenges exist in the translation of such lipidomic data to clinical applications. These relate to the reproducibility, accuracy, and precision of lipid quantitation, study design, sample handling, and data sharing. This position paper emerged from a workshop that initiated a community-led process to elaborate and define a set of generally accepted guidelines for quantitative MS-based lipidomics of blood plasma or serum, with harmonization of data acquired on different instrumentation platforms across independent laboratories as an ultimate goal. We hope that other fields may benefit from and follow such a precedent.
- Published
- 2018
28. Assessing chemotherapeutic effectiveness using a paper-based tumor model
- Author
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Matthew W. Boyce, Gabriel J. LaBonia, Amanda B. Hummon, and Matthew R. Lockett
- Subjects
Paper ,0301 basic medicine ,Fluorescence-lifetime imaging microscopy ,Scaffold ,Oxygen gradient ,Cell ,Cell Culture Techniques ,Cell Count ,Nanotechnology ,Models, Biological ,Biochemistry ,Article ,Analytical Chemistry ,Diffusion ,03 medical and health sciences ,0302 clinical medicine ,Neoplasms ,Cell density ,Electrochemistry ,medicine ,Humans ,Environmental Chemistry ,Spectroscopy ,Prolonged incubation ,Chemistry ,Paper based ,HCT116 Cells ,Cell biology ,Oxygen ,030104 developmental biology ,medicine.anatomical_structure ,030220 oncology & carcinogenesis - Abstract
In vitro models for screening new cancer chemotherapeutics often rely on two-dimensional cultures to predict therapeutic potential. Unfortunately, the predictive power of these models is limited, as they fail to recapitulate the complex three-dimensional environments in tumors that promote the development of a chemoresistant phenotype. In this study, we describe the preparation and characterization of paper-based cultures (PBC) engineered to assess chemotherapeutic effectiveness in three dimensional, diffusion-limited environments. Similar environments are found within poorly vascularized tumors. These cultures, which are assembled by stacking together cell-laden paper scaffolds to yield thick tissue-like structures, generate monotonic gradients vertically through the culture and provide distinct chemical environments for each scaffold. After prolonged incubation, the scaffolds can simply be peeled apart and analyzed to relate cellular responses to the chemical environment experienced at that scaffold. Through fluorescence imaging, viable and proliferative cell populations were mapped within a stacked culture and found to be most abundant in scaffolds close to the nutrient-rich medium. By adjusting the cell density, we modulated the spatiotemporal evolution of oxygen gradients across the cultures and correlated these environmental changes with cellular sensitivity to SN-38 exposure. From these results, we showed that differences in the oxygen gradients produced cellular populations with significantly different chemosensitivities. Through this work, we highlight PBCs ability to serve as an analytical model capable of determining chemotherapeutic effectiveness under a range of chemical environments.
- Published
- 2017
29. An American Physiological Society cross-journal Call for Papers on 'The Physiology of Obesity'
- Author
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Sue C. Bodine, Heddwen L. Brooks, Hilary A. Coller, Ana I. Domingos, Mark R. Frey, Barbara E. Goodman, Thomas R. Kleyman, Merry L. Lindsey, Rory E. Morty, Ole H. Petersen, Jan-Marino Ramírez, Liliana Schaefer, Morten B. Thomsen, and Gina L. C. Yosten
- Subjects
Pulmonary and Respiratory Medicine ,Editorial ,Physiology ,Physiology (medical) ,Humans ,Cell Biology ,Obesity ,United States - Published
- 2022
30. Generational Message. Helena Radlińska’s Papers in 'Pismo Młodych' (1943)
- Author
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Izabela Kamińska-Jatczak, Uniwersytet Łódzki, and izabela.kaminska@now.uni.lodz.pl
- Subjects
Embryology ,II wojna światowa ,II World War ,Helena Radlińska ,Gray Ranks Assault Groups ,Grupy Szturmowe Szarych Szeregów ,war journalism ,Cell Biology ,generational message ,Anatomy ,przekaz pokoleniowy ,publicystyka wojenna ,Developmental Biology - Abstract
The article presents the main themes of journalistic texts probably written by Helena Radlińska, and published on the pages of the underground magazine ‘Pismo Młodych’ addressed to the Gray Ranks Assault Groups. The selected topics, referring to the main concepts of Radlińska’s social pedagogy, were distinguished as a result of a qualitative content analysis. The synthesis of the content addressed to the fighting youth and their instructors, leads to a conclusion regarding the generational message that Radlińska directed to the younger generation fighting against the invader during World War II. The text reveals a less known part of the journalistic work of the scientist from the war period. Artykuł prezentuje główne tematy tekstów publicystycznych, napisanych prawdopodobnie przez Helenę Radlińską na łamach pisma konspiracyjnego „Pismo Młodych”, adresowanego do Grup Szturmowych Szarych Szeregów. Wyłonione tematy, odwołujące się do głównych pojęć pedagogiki społecznej Radlińskiej, zostały wyodrębnione w efekcie przeprowadzonej jakościowej analizy treści. Synteza treści adresowanych do młodzieży walczącej i ich instruktorów prowadzi do konkluzji dotyczącej przekazu pokoleniowego, jaki Radlińska kierowała do młodszej generacji walczącej z okupantem podczas II wojny światowej. Tekst odsłania mniej znaną kartę twórczości publicystycznej uczonej z okresu wojennego.
- Published
- 2022
31. Sterility of Gauze Packed in One and Three Layer Layer Parchment Paper
- Author
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Vincentia Rizke Ciptaningtyas, Endang Sri Lestari, and Saskia Biyakto Putri
- Subjects
Embryology ,lcsh:R5-920 ,Materials science ,Parchment ,Sterility ,sterility ,Significant difference ,Cell Biology ,Sterilization (microbiology) ,sterile gauze ,Anatomy ,Composite material ,lcsh:Medicine (General) ,Developmental Biology ,parchment paper - Abstract
Intoduction: Sterile gauze is one of the medical devices that are often used to prevent infection. Several things, one of them is materials for packaging, can affect the sterility of gauze. Parchment paper is one of the packaging materials for sterilization. The study was done to analyze the sterility of gauze packed with parchment paper one layer and three layers on the growth of microorganism.Method: This study was an experimental study with a quasi-experimental design. Samples were 60 gauze packed with one layer parchment paper and 60 gauze packed with three layers of parchment paper. The sterility of sterile gauze tested at weeks 0, 2 and 4. Results: There was no significant difference in the sterility of gauze packed with 1 layer of parchment paper on the growth of microorganisms at 0, 2, 4 weeks (p = 0.126). No significant difference in the sterility of sterile gauze packed with 3 layers of parchment paper at 0, 2, 4 weeks (p = 0.675). There was a significant difference in the sterility of sterile gauze packed with parchment paper 1 layer and 3 layers on the growth of microorganisms (p = 0.002).Conclusion There is a significant difference of sterile gauze packed with parchment paper 1 layer and 3 layers against the growth of microorganisms. The sterility of the packaged sterile gauze with a layer of parchment paper is better than the three layers.
- Published
- 2017
32. The leading role of pathology in assessing the somatic molecular alterations of cancer: Position Paper of the European Society of Pathology
- Author
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Matias-Guiu, Xavier, Stanta, Giorgio, Carneiro, Fátima, Ryska, Ales, Hoefler, Gerald, Moch, Holger, European Society of Pathology (ESP), University of Zurich, and Matias-Guiu, Xavier
- Subjects
0301 basic medicine ,Pathology ,medicine.medical_specialty ,Pre-analytical issues ,Somatic cell ,Medical laboratory ,610 Medicine & health ,Context (language use) ,Pathology and Forensic Medicine ,1307 Cell Biology ,03 medical and health sciences ,0302 clinical medicine ,10049 Institute of Pathology and Molecular Pathology ,Neoplasms ,1312 Molecular Biology ,medicine ,Biomarkers, Tumor ,Molecular diagnostics ,Humans ,Patologia molecular ,Pathology, Molecular ,Precision Medicine ,Càncer ,Molecular Biology ,Cancer ,Molecular pathology ,business.industry ,Cell Biology ,General Medicine ,medicine.disease ,Molecular analysis ,2734 Pathology and Forensic Medicine ,Review and Perspectives ,Europe ,Pathologists ,030104 developmental biology ,030220 oncology & carcinogenesis ,Scientific domain ,Commentary ,Position paper ,business ,Integrative pathology - Abstract
Molecular pathology is an essential part of pathology complementing conventional morphological tools to obtain a correct integrated diagnosis with appropriate assessment of prognosis and prediction of response to therapy, particularly in cancer. There is a concern about the situation of molecular pathology in some areas of Europe, namely, regarding the central role of pathologists in assessing somatic genomic alterations in cancer. In some countries, there are attempts that other laboratory medicine specialists perform the molecular analysis of somatic alterations in cancer, particularly now when next generation sequencing (NGS) is incorporated into clinical practice. In this scenario, pathologists may play just the role of "tissue providers," and other specialists may take the lead in molecular analysis. Geneticists and laboratory medicine specialists have all background and skills to perform genetic analysis of germline alterations in hereditary disorders, including familial forms of cancers. However, interpretation of somatic alterations of cancer belongs to the specific scientific domain of pathology. Pathologists are necessary to guarantee the quality of the results, for several reasons: (1) The identified molecular alterations should be interpreted in the appropriate morphologic context, since most of them are context-specific; (2) pre-analytical issues must be taken into consideration; (3) it is crucial to check the proportion of tumor cells in the sample subjected to analysis and presence of inflammatory infiltrate and necrosis should be monitored; and 4) the role of pathologists is crucial to select the most appropriate methods and to control the turnaround time in which the molecular results are delivered in the context of an integrated diagnosis. Obviously, there is the possibility of having core facilities for NGS in a hospital to perform the sequence analysis that are open to other specialties (microbiologists, geneticists), but also in this scenario, pathologists should have the lead in assessing somatic alterations of cancer. In this article, we emphasize the importance of interpreting somatic molecular alterations of the tumors in the context of morphology. In this Position Paper of the European Society of Pathology, we strongly support a central role of pathology departments in the process of analysis and interpretation of somatic molecular alterations in cancer. The proposal for this position paper was approved by the Publication Task Force of the European Society of Pathology (ESP), and this approval was subsequently verified by the Council of ESP. We thank Mar Varela, Dolors Cuevas, Ana Velasco and Jordi Temprana for help in writing the manuscript.
- Published
- 2019
33. Can citation metrics predict the true impact of scientific papers?
- Author
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Aroeira, Rita I., Castanho, Miguel A. R. B., and Repositório da Universidade de Lisboa
- Subjects
0301 basic medicine ,Paper ,Computer science ,Biochemistry ,03 medical and health sciences ,0302 clinical medicine ,Humans ,Molecular Biology ,Scientific evaluation ,Impact factor ,Research ,Scientific production ,Experimental data ,Cell Biology ,Data science ,Research Personnel ,Impact ,030104 developmental biology ,Work (electrical) ,Bibliometrics ,030220 oncology & carcinogenesis ,Test set ,Number of citations ,Criticism ,Metrics ,Citation ,Value (mathematics) - Abstract
© 2020 Federation of European Biochemical Societies, Bibliometric quantification is frequently used as metrics for the evaluation of the scientific performance of researchers and institutions. The researchers’ merit is usually assessed by the analysis of quantitative parameters such as the number of publications, the impact factor of journals, the total number of citations, or the h-index, although the limitations in translating these indicators into the impact of the outcome of scientific production are a matter of harsh criticism. To assess, based on factual evidences, the validity of traditional bibliometric analyses to conclude on the impact of papers to advance the state of the art, we carried out an innovative methodology on selected publications (test set). This methodology is based on identifying those citations of the test set papers that truly embed the methods, concepts, or hypotheses to build new knowledge and formulate conclusions. The results show that the percentage of citations that reflect the real impact of the papers of the test set has an average value of 12.4% of total citations and is not related to the impact factor of the journal where the test set papers were published. In conclusion, our analysis demonstrates factually, using experimental data, the total failure of using quantitative bulk citation analyses to conclude on the scientific impact of publications. Only a careful analysis of how the work described in papers was embedded on the subsequent work and/or conclusions of others can tell about the real contribution of a published work to the development of new knowledge and advancement of science., This work was supported by ‘La Caixa’ Foundation (grant reference: IMM/BPD/107-2018).
- Published
- 2019
34. Biological membranes in EV biogenesis, stability, uptake, and cargo transfer: an ISEV position paper arising from the ISEV membranes and EVs workshop
- Author
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Russell, Ashley E, Sneider, Alexandra, Witwer, Kenneth W, Bergese, Paolo, Bhattacharyya, Suvendra N, Cocks, Alexander, Cocucci, Emanuele, Erdbrügger, Uta, Falcon-Perez, Juan M, Freeman, David W, Gallagher, Thomas M, Hu, Shuaishuai, Huang, Yiyao, Jay, Steven M, Kano, Shin-Ichi, Lavieu, Gregory, Leszczynska, Aleksandra, Llorente, Alicia M, Lu, Quan, Mahairaki, Vasiliki, Muth, Dillon C, Noren Hooten, Nicole, Ostrowski, Matias, Prada, Ilaria, Sahoo, Susmita, Schøyen, Tine Hiorth, Sheng, Lifu, Tesch, Deanna, Van Niel, Guillaume, Vandenbroucke, Roosmarijn E, Verweij, Frederik J, Villar, Ana V, Wauben, Marca, Wehman, Ann M, Yin, Hang, Carter, David Raul Francisco, Vader, Pieter, LS Celbiologie-Algemeen, dB&C I&I, Sub General Pharmaceutics, Universidad de Cantabria, LS Celbiologie-Algemeen, dB&C I&I, and Sub General Pharmaceutics
- Subjects
0301 basic medicine ,fusion ,Biogenesis ,Exosomes ,Extracellular vesicles ,Fusion ,Membranes ,Uptake ,Nanoparticles ,purl.org/becyt/ford/1 [https] ,0302 clinical medicine ,IMMERSION DEPTH ,Medicine and Health Sciences ,MEDIATED ENDOCYTOSIS ,GENE-EXPRESSION ,lcsh:Cytology ,3. Good health ,ISEV workshop ,Membrane ,membranes ,030220 oncology & carcinogenesis ,uptake ,technology ,Position Paper ,position paper ,Histology ,Membrane biology ,Nanotechnology ,DENDRITIC CELLS ,03 medical and health sciences ,VDP::Medisinske Fag: 700 ,lcsh:QH573-671 ,purl.org/becyt/ford/1.6 [https] ,Biology ,paper ,MULTIVESICULAR BODIES ,Biology and Life Sciences ,Biological membrane ,Cell Biology ,biogenesis ,EMERGING ROLE ,biogenesisfusion ,EXOSOME SECRETION ,FAS LIGAND ,position ,030104 developmental biology ,PLASMA-MEMBRANE ,Position paper ,Business - Abstract
Paracrine and endocrine roles have increasingly been ascribed to extracellular vesicles (EVs) generated by multicellular organisms. Central to the biogenesis, content, and function of EVs are their delimiting lipid bilayer membranes. To evaluate research progress on membranes and EVs, the International Society for Extracellular Vesicles (ISEV) conducted a workshop in March 2018 in Baltimore, Maryland, USA, bringing together key opinion leaders and hands-on researchers who were selected on the basis of submitted applications. The workshop was accompanied by two scientific surveys and covered four broad topics: EV biogenesis and release; EV uptake and fusion; technologies and strategies used to study EV membranes; and EV transfer and functional assays. In this ISEV position paper, we synthesize the results of the workshop and the related surveys to outline important outstanding questions about EV membranes and describe areas of consensus. The workshop discussions and survey responses reveal that while much progress has been made in the field, there are still several concepts that divide opinion. Good consensus exists in some areas, including particular aspects of EV biogenesis, uptake and downstream signalling. Areas with little to no consensus include EV storage and stability, as well as whether and how EVs fuse with target cells. Further research is needed in these key areas, as a better understanding of membrane biology will contribute substantially towards advancing the field of extracellular vesicles. Fil: Russell, Ashley E.. University Johns Hopkins; Estados Unidos Fil: Sneider, Alexandra. University Johns Hopkins; Estados Unidos Fil: Witwer, Kenneth W.. University Johns Hopkins; Estados Unidos Fil: Bergese, Paolo. Università Degli Studi Di Brescia; Italia Fil: Bhattacharyya, Suvendra N.. Indian Institute of Chemical Biology; India Fil: Cocks, Alexander. Cardiff University; Reino Unido Fil: Cocucci, Emanuele. Ohio State University; Estados Unidos Fil: Erdbrügger, Uta. University of Virginia; Estados Unidos Fil: Falcon Perez, Juan M.. Ikerbasque Basque Foundation for Science; España Fil: Freeman, David W.. National Institute On Aging National Institute for Helth ; Estados Unidos Fil: Gallagher, Thomas M.. Loyola University Of Chicago; Estados Unidos Fil: Hu, Shuaishuai. Technological University Dublin; Irlanda Fil: Huang, Yiyao. University Johns Hopkins; Estados Unidos. Southern Medical University; China Fil: Jay, Steven M.. University of Maryland; Estados Unidos Fil: Kano, Shin-ichi. The University of Alabama at Birmingham School of Medicine; Estados Unidos Fil: Lavieu, Gregory. Institut Curie; Francia Fil: Leszczynska, Aleksandra. University of California at San Diego; Estados Unidos Fil: Llorente, Alicia M.. Oslo University Hospital; Noruega Fil: Lu, Quan. Harvard University. Harvard School of Public Health; Estados Unidos Fil: Mahairaki, Vasiliki. University Johns Hopkins; Estados Unidos Fil: Muth, Dillon C.. University Johns Hopkins; Estados Unidos Fil: Noren Hooten, Nicole. National Institute On Aging National Institute for Helth ; Estados Unidos Fil: Ostrowski, Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina Fil: Prada, Ilaria. Consiglio Nazionale delle Ricerche; Italia Fil: Sahoo, Susmita. Icahn School of Medicine at Mount Sinai ; Estados Unidos Fil: Schøyen, Tine Hiorth. Uit The Arctic University Of Norway; Noruega. University Johns Hopkins; Estados Unidos Fil: Sheng, Lifuy. University of Washington. School of Medicine; Estados Unidos Fil: Tesch, Deanna. Shaw University; Estados Unidos Fil: Van Niel, Guillaume. No especifíca; Fil: Vandenbroucke, Roosmarijn E.. University of Ghent; Bélgica Fil: Verweij, Frederik J.. No especifíca; Fil: Villar, Ana V.. Universidad de Cantabria; España Fil: Wauben, Marca. University of Utrecht; Países Bajos Fil: Wehman, Ann M.. Universität Würzburg; Alemania Fil: Ardavan, Arzhang. Peking University; ; China Fil: Carter, David Raul Francisco. Oxford Brookes University; Reino Unido Fil: Vader, Pieter. University Medical Center Utrecht; Países Bajos
- Published
- 2019
- Full Text
- View/download PDF
35. Call for Special Issue Papers: Cellular Reprogramming 25th AnniversaryDeadline for Manuscript Submission: April 30, 2023
- Author
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Carlos-Filipe Pereira
- Subjects
Cell Biology ,Developmental Biology ,Biotechnology - Published
- 2022
36. Paper-Based Microchip Electrophoresis Enabled First Point-of-Care Diagnostic Test for Beta-Thalassemia
- Author
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Ran An, Alireza Avanaki, Priyaleela Thota, Sai Nemade, Amrish Mehta, and Umut A. Gurkan
- Subjects
Immunology ,Cell Biology ,Hematology ,Biochemistry - Published
- 2022
37. Editorial - announcing the winners of the SEB-Wiley-TPJ awards for outstanding TPJ papers published in 2021
- Author
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Lee Sweetlove
- Subjects
Publications ,Genetics ,Awards and Prizes ,Cell Biology ,Plant Science - Published
- 2022
38. What we are doing to help researchers publish their papers in our journal
- Author
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Gordon Mallarkey and Matthew Thorne
- Subjects
Publishing ,Bibliometrics ,Biochemistry (medical) ,Humans ,Cell Biology ,General Medicine ,Biochemistry - Published
- 2022
39. Chronic neck pain and respiratory dysfunction: a review paper
- Author
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Shruti P. Nair, Chaitrali S. Panchabhai, and Vrushali Panhale
- Subjects
Embryology ,General Medicine ,Cell Biology ,Anatomy ,Developmental Biology - Abstract
Background Neck pain is one of the most frequently reported musculoskeletal complaints among adults; its prevalence in the world is ranging from 16.7 to 75.1%. It can have an impact on a person’s physical, psychological, and social well-being. Along with pain, disability, muscle weakness, and alterations in the posture, neck pain patients are likely to develop affection of the respiratory function as reported in numerous studies. However, these patients are primarily managed with a musculoskeletal perspective with little or no emphasis to the changes observed in the respiratory system. There is a paucity of literature evaluating the need for respiratory rehabilitation in these patients. Main body All relevant published literature related to respiratory dysfunction in patients with chronic neck pain were critically reviewed in this study. Patients having chronic neck pain were found to have alterations in respiratory function in terms of reduced lung volumes, reduced chest mobility, and decreased respiratory muscle strength. Various factors such as decreased cervical range of motion, decreased strength of deep neck flexors and extensors, forward head posture, and pain are known to cause these dysfunctions. Respiratory system intervention in the form of breathing re-education and respiratory exercises are significantly proven to improve treatment outcomes. Conclusion There is limited literature relating to respiratory dysfunction and its management in neck pain patients. Incorporation of both respiratory and musculoskeletal assessments can enhance their treatment outcomes. Additionally, it can be suggested to consider intervention in the form of respiratory rehabilitation while strategizing treatment goals for these patients.
- Published
- 2022
40. Fifteen years of publishing the papers on the impact of biological factors on hydrological processes in Biologia
- Author
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Kálmán Rajkai, Csilla Farkas, Ľubomír Lichner, Miroslav Tesař, and Katarína Hegedüšová
- Subjects
Land use ,business.industry ,Environmental resource management ,Climate change ,Cell Biology ,Plant Science ,Biochemistry ,Water scarcity ,Water resources ,Greenhouse gas ,Scale (social sciences) ,Soil retrogression and degradation ,Vadose zone ,Genetics ,Environmental science ,Animal Science and Zoology ,business ,Molecular Biology ,Ecology, Evolution, Behavior and Systematics - Abstract
Special issue on the impact of biological factors on hydrological processes, edited by the authors of this Foreword, brings together 11 full-text papers on these complex interactions, ranging in scale from storing the water by lichen species to competing the nations for increasingly scarce water resources in securitized transboundary watersheds. In addition to these “boundary-scale” biohydrology papers, 4 papers assess the impact of land use on soil properties, 3 papers estimate the effect of biological factors on the downward and upward movements of water in the vadose zone of soil, and 2 papers evaluated the amount of greenhouse gas emissions from agriculturally utilized soil and forest fires. We would like to conclude by expressing our hope that the papers published in the present special issue will act as a stimulus for initiating new research projects and contribute to finding solutions to the global threats of water scarcity and soil degradation. It is absolutely crucial to increase our chances of surviving the current climate change and to reach a more sustainable society in the future.
- Published
- 2020
41. All-inkjet-printed MoS2 field-effect transistors on paper for low-cost and flexible electronics
- Author
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Jin Ju Chen, Zhe sheng Feng, Zhi Jiang, Evgeniya Sheremet, Enrico Sowade, Raul D. Rodriguez, Long Chen, Zhao quan Xu, Yan Wang, and Reinhard R. Baumann
- Subjects
Materials science ,Materials Science (miscellaneous) ,Hardware_PERFORMANCEANDRELIABILITY ,02 engineering and technology ,Integrated circuit ,Substrate (printing) ,010402 general chemistry ,01 natural sciences ,law.invention ,law ,Hardware_INTEGRATEDCIRCUITS ,Electronics ,Electrical and Electronic Engineering ,Physical and Theoretical Chemistry ,business.industry ,Transistor ,Cell Biology ,021001 nanoscience & nanotechnology ,Atomic and Molecular Physics, and Optics ,Flexible electronics ,0104 chemical sciences ,Electrode ,Optoelectronics ,Field-effect transistor ,0210 nano-technology ,business ,Layer (electronics) ,Biotechnology - Abstract
All-inkjet-printing of transistors has received much attention for low cost and flexible integrated circuits. However, most flexible field-effect transistors (FETs) based on the emerging two-dimensional materials suffer from the high cost of substrate and electrode materials. The requirements for high-temperature synthesis and precise control in processing add another layer of complexity. To overcome these issues, low-cost flexible paper-based MoS2 FETs were fabricated by inkjet printing of MoS2 channel materials on paper. Additionally, we proposed and achieved the mask-less and low-temperature formation of source and drain electrodes on paper using in-situ selective-area copper reduction. A low sub-threshold swing of 80 mV/dec, high on/off ratio of 105, and very high turn-on current (Ion) of 200 μA of the paper-based flexible MoS2 FETs were demonstrated using the proposed low-cost and facile all-inkjet-printing technique. The all-inkjet-printing technique assisted by in-situ copper reduction opens new opportunities for low-cost and batch fabrication of paper-based electronic devices in ambient conditions.
- Published
- 2020
42. Digital magic, or the dark arts of the 21stcentury—how can journals and peer reviewers detect manuscripts and publications from paper mills?
- Author
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Jennifer A. Byrne and Jana Christopher
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0303 health sciences ,Magic (illusion) ,Computer science ,business.industry ,Scale (chemistry) ,media_common.quotation_subject ,030302 biochemistry & molecular biology ,Biophysics ,ComputerApplications_COMPUTERSINOTHERSYSTEMS ,Cell Biology ,Public relations ,Biochemistry ,The arts ,Product (business) ,03 medical and health sciences ,Structural Biology ,Genetics ,Quality (business) ,Contract cheating ,business ,Molecular Biology ,Publication ,030304 developmental biology ,Research data ,media_common - Abstract
In recent years, it has been proposed that unrealistic requirements for academics and medical doctors to publish in scientific journals, combined with monetary publication rewards, have led to forms of contract cheating offered by organizations known as paper mills. Paper mills are alleged to offer products ranging from research data through to ghostwritten fraudulent or fabricated manuscripts and submission services. While paper mill operations remain poorly understood, it seems likely that paper mills need to balance product quantity and quality, such that they produce or contribute to large numbers of manuscripts that will be accepted for publication. Producing manuscripts at scale may be facilitated by the use of manuscript templates, which could give rise to shared features such as textual and organizational similarities, the description of highly generic study hypotheses and experimental approaches, digital images that show evidence of manipulation and/or reuse, and/or errors affecting verifiable experimental reagents. Based on these features, we propose practical steps that editors, journal staff, and peer reviewers can take to recognize and respond to research manuscripts and publications that may have been produced with undeclared assistance from paper mills.
- Published
- 2020
43. Nucleic acid sample preparation from whole blood in a paper microfluidic device using isotachophoresis
- Author
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Andrew T. Bender, Duy N. Ngyuen, Benjamin Sullivan, Jonathan D. Posner, and Jane Yuqian Zhang
- Subjects
Paper ,Protein digestion ,Clinical Biochemistry ,Blood fractionation ,Recombinase Polymerase Amplification ,030226 pharmacology & pharmacy ,01 natural sciences ,Biochemistry ,Article ,Analytical Chemistry ,03 medical and health sciences ,0302 clinical medicine ,Lab-On-A-Chip Devices ,Nucleic Acids ,Humans ,Nucleic Acid Amplification Tests ,Sample preparation ,Chromatography ,Isotachophoresis ,Chemistry ,010401 analytical chemistry ,Proteolytic enzymes ,Equipment Design ,Cell Biology ,General Medicine ,Microfluidic Analytical Techniques ,0104 chemical sciences ,Nucleic acid ,Electrophoresis, Polyacrylamide Gel ,Nucleic Acid Amplification Techniques - Abstract
Nucleic acid amplification tests (NAATs) are a crucial diagnostic and monitoring tool for infectious diseases. A key procedural step for NAATs is sample preparation: separating and purifying target nucleic acids from crude biological samples prior to nucleic acid amplification and detection. Traditionally, sample preparation has been performed with liquid- or solid-phase extraction, both of which require multiple trained user steps and significant laboratory equipment. The challenges associated with sample preparation have limited the dissemination of NAAT point-of-care diagnostics in low resource environments, including low- and middle-income countries. We report on a paper-based device for purification of nucleic acids from whole blood using isotachophoresis (ITP) for point-of-care NAATs. We show successful extraction and purification of target nucleic acids from large volumes (33 µL) of whole human blood samples with no moving parts and few user steps. Our device utilizes paper-based buffer reservoirs to fully contain the liquid ITP buffers and does not require complex filling procedures, instead relying on the natural wicking of integrated paper membranes. We perform on-device blood fractionation via filtration to remove leukocytes and erythrocytes from our sample, followed by integrated on-paper proteolytic digestion of endogenous plasma proteins to allow for successful isotachophoretic extraction. Paper-based isotachophoresis purifies and concentrates target nucleic acids that are added directly to recombinase polymerase amplification (RPA) reactions. We show consistent amplification of input copy concentrations of as low as 3 × 103 copies nucleic acid per mL input blood with extraction and purification taking only 30 min. By employing a paper architecture, we are able to incorporate these processes in a single, robust, low-cost design, enabling the direct processing of large volumes of blood, with the only intermediate user steps being the removal and addition of tape. Our device represents a step towards a simple, fully integrated sample preparation system for nucleic acid amplification tests at the point-of-care.
- Published
- 2021
44. An Activity-Staining Method on Filtration Paper Enables High-Throughput Screening of Temperature-Sensitive and Inactive Mutations of Rice α-Amylase for Improvement of Rice Grain Quality
- Author
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Masaru Nakata, Takeshi Yamaguchi, Yuriko Nakata, Masaharu Kuroda, Rieko Hirai-Kimura, and Hiromoto Yamakawa
- Subjects
Paper ,0106 biological sciences ,0301 basic medicine ,Physiology ,Starch ,Mutant ,Mutagenesis (molecular biology technique) ,Plant Science ,medicine.disease_cause ,Polymerase Chain Reaction ,01 natural sciences ,Endosperm ,03 medical and health sciences ,chemistry.chemical_compound ,Grain quality ,medicine ,Protein Isoforms ,Amylase ,Escherichia coli ,Plant Proteins ,chemistry.chemical_classification ,biology ,Chemistry ,business.industry ,Temperature ,food and beverages ,Oryza ,Cell Biology ,General Medicine ,Recombinant Proteins ,High-Throughput Screening Assays ,Biotechnology ,030104 developmental biology ,Enzyme ,Biochemistry ,Mutation ,Seeds ,biology.protein ,alpha-Amylases ,business ,Filtration ,010606 plant biology & botany - Abstract
α-Amylase is a starch-hydrolyzing enzyme (EC 3.2.1.1) indispensable for germination of cereal seeds, but it is also expressed during the ripening stage. Previous studies demonstrated that the enzyme is activated in developing rice seeds under extremely hot weather and triggers a loss of grain quality by hindering the accumulation of storage starch in the endosperm. Since inactive or, preferably, heat-labile α-amylases are preferable for breeding premium rice, we developed a method for rapid screening of inactive and temperature-sensitive mutants of the enzyme by combining the random mutagenesis by error-prone PCR and an on-filter activity test of the recombinant enzyme expressed by Escherichia coli. This technique was applied to a major α-amylase in the developing seed, Amy3D, and the activity of the isolated mutant enzymes was verified with both the bacteria-expressed recombinant proteins and the extract from the endosperm overexpressing each of them. Then, we identified several substitutions leading to loss of the activity of amino acid residues (Leu28, Asp112, Cys149, Trp201, Asp204, Gly295, Leu300 and Cys342), as well as a variety of heat-sensitive substitutions of Asp83, Asp187 and Glu252. Furthermore, variations of the heat-labile enzymes were created by combining these heat-sensitive mutations. The effects of the respective mutations and their relationship to the structure of the enzyme molecule are discussed.
- Published
- 2017
45. Scientific Papers by Developmental Biologists in Japan
- Author
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Tsutomu Nohno and Hideyo Ohuchi
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Cell Biology ,Molecular Biology ,Developmental Biology - Abstract
We have assembled ten interesting manuscripts submitted by developmental biologists in Japan [...]
- Published
- 2023
46. Redesigning the Workflow Process to Upload Paper Consents into the Electronic Health Record
- Author
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Nancy Tang, Christopher Bourne, Pam Kostaras, Daniel Surette, Kaitlen Reyes, Bethany King, Kimberly Craig, Linda Ramsdell, and Julie Porter
- Subjects
Transplantation ,Molecular Medicine ,Immunology and Allergy ,Cell Biology ,Hematology - Published
- 2023
47. Where do we aspire to publish? A position paper on scientific communication in biochemistry and molecular biology
- Author
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Alicia J. Kowaltowski, Fabio Luis Forti, Roberto Kopke Salinas, S C Farah, Nicolas C. Hoch, Ricardo J. Giordano, Ohara Augusto, Glaucia Mendes Souza, A. M. Da-Silva, P. Di Mascio, Cypreste Oliveira, Walter R. Terra, Sayuri Miyamoto, Claudiana Lameu, M. J. M. Alves, Daniela S. Basseres, Alexandre Bruni-Cardoso, Sandro R. Marana, Hugo A. Armelin, Hernan Chaimovich, João C. Setubal, Deborah Schechtman, Marisa Helena Gennari de Medeiros, Sergio Verjovski-Almeida, Eduardo M. Reis, Maria Teresa Machini, Suely Lopes Gomes, Shirley Schreier, F J Gueiros Filho, Henning Ulrich, Bettina Malnic, Walter Colli, Mari Cleide Sogayar, P. Colepicolo Neto, Carlos Takeshi Hotta, Graziella E. Ronsein, Mauricio S. Baptista, Iolanda M. Cuccovia, Daniela R. Truzzi, Leticia Labriola, Flavia Vischi Winck, Carmen Veríssima Ferreira, Guilherme Menegon Arantes, Flavia Carla Meotti, Bianca Zingales, Regina L. Baldini, Nadja C. de Souza-Pinto, and Etelvino J. H. Bechara
- Subjects
0301 basic medicine ,Physiology ,media_common.quotation_subject ,Immunology ,Biophysics ,MEDLINE ,Ocean Engineering ,Biochemistry ,03 medical and health sciences ,Presentation ,0302 clinical medicine ,Political science ,PRÉ-IMPRESSOS ,Humans ,General Pharmacology, Toxicology and Pharmaceutics ,Composition (language) ,Publication ,lcsh:QH301-705.5 ,Molecular Biology ,media_common ,Publishing ,lcsh:R5-920 ,business.industry ,General Neuroscience ,Research ,Subject (documents) ,Cell Biology ,General Medicine ,Concepts and Comments ,Scientific journals ,Open access ,Molecular biology ,030104 developmental biology ,Scientific editing ,lcsh:Biology (General) ,030220 oncology & carcinogenesis ,Pre-prints ,Position paper ,Periodicals as Topic ,business ,lcsh:Medicine (General) ,Scientific communication ,Brazil - Abstract
The scientific publication landscape is changing quickly, with an enormous increase in options and models. Articles can be published in a complex variety of journals that differ in their presentation format (online-only or in-print), editorial organizations that maintain them (commercial and/or society-based), editorial handling (academic or professional editors), editorial board composition (academic or professional), payment options to cover editorial costs (open access or pay-to-read), indexation, visibility, branding, and other aspects. Additionally, online submissions of non-revised versions of manuscripts prior to seeking publication in a peer-reviewed journal (a practice known as pre-printing) are a growing trend in biological sciences. In this changing landscape, researchers in biochemistry and molecular biology must re-think their priorities in terms of scientific output dissemination. The evaluation processes and institutional funding for scientific publications should also be revised accordingly. This article presents the results of discussions within the Department of Biochemistry, University of Sao Paulo, on this subject.
- Published
- 2019
48. A practical algorithmic approach to mature aggressive B cell lymphoma diagnosis in the double/triple hit era. Selecting cases, matching clinical benefit. A position paper from the Italian Group of Haematopathology (G.I.E.)
- Author
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Maurilio Ponzoni, Fabio Facchetti, Claudio Tripodo, Maria Raffaella Ambrosio, Alberto Zamo, Stefano Lazzi, Arianna Di Napoli, Domenico Novero, Marco Lucioni, Marco Paulli, Claudio Agostinelli, Elena Sabattini, Alessia Carbone, Stefano Ascani, Stefano Pileri, Luigi Ruco, Lorenzo Leoncini, D. Remotti, Di Napoli, A., Remotti, D., Agostinelli, C., Ambrosio, M. R., Ascani, S., Carbone, A., Facchetti, F., Lazzi, S., Leoncini, L., Lucioni, M., Novero, D., Pileri, S., Ponzoni, M., Sabattini, E., Tripodo, C., Zamo, A., Paulli, M., Ruco, L., Di Napoli A., Remotti D., Agostinelli C., Ambrosio M.R., Ascani S., Carbone A., Facchetti F., Lazzi S., Leoncini L., Lucioni M., Novero D., Pileri S., Ponzoni M., Sabattini E., Tripodo C., Zamo A., Paulli M., and Ruco L.
- Subjects
0301 basic medicine ,medicine.medical_specialty ,Matching (statistics) ,Lymphoma, B-Cell ,Lymphoma ,double hit ,Computer science ,MYC ,Double hit ,Fluorescence ,Pathology and Forensic Medicine ,Immunophenotyping ,03 medical and health sciences ,0302 clinical medicine ,FISH ,Diagnosis ,medicine ,Practical algorithm ,Humans ,Intensive care medicine ,B-cell lymphoma ,Molecular Biology ,In Situ Hybridization ,In Situ Hybridization, Fluorescence ,HGBL ,Brief Report ,B-Cell ,Treatment options ,Correction ,DLBCL ,Algorithms ,Cell Biology ,General Medicine ,Diagnosis, DLBCL, Double hit, FISH, HGBL, MYC ,medicine.disease ,Optimal management ,Molecular analysis ,030104 developmental biology ,030220 oncology & carcinogenesis ,Position paper ,Proper treatment ,Diagnosi ,Human - Abstract
An accurate diagnosis of clinically distinct subgroups of aggressive mature B cell lymphomas is crucial for the choice of proper treatment. Presently, precise recognition of these disorders relies on the combination of morphological, immunophenotypical, and cytogenetic/molecular features. The diagnostic workup in such situations implies the application of costly and time-consuming analyses, which are not always required, since an intensified treatment option is reasonably reserved to fit patients. The Italian Group of Haematopathology proposes herein a practical algorithm for the diagnosis of aggressive mature B cell lymphomas based on a stepwise approach, aimed to select cases deserving molecular analysis, in order to optimize time and resources still assuring the optimal management for any patient.
- Published
- 2019
49. sj-pdf-1-jcb-10.1177_0271678X221119760 - Supplemental material for Transfer function analysis of dynamic cerebral autoregulation: a CARNet white paper 2022 update
- Author
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Panerai, Ronney B, Brassard, Patrice, Burma, Joel S, Castro, Pedro, Claassen, Jurgen AHR, van Lieshout, Johannes J, Liu, Jia, Lucas, Samuel JE, Minhas, Jatinder S, Mitsis, Georgios D, Nogueira, Ricardo C, Ogoh, Shigehiko, Payne, Stephen J, Rickards, Caroline A, Robertson, Andrew D, Rodrigues, Gabriel D, Smirl, Jonathan D, and Simpson, David M
- Subjects
110320 Radiology and Organ Imaging ,FOS: Clinical medicine ,FOS: Biological sciences ,Medicine ,Cell Biology ,110305 Emergency Medicine ,110306 Endocrinology ,Biochemistry ,69999 Biological Sciences not elsewhere classified ,110904 Neurology and Neuromuscular Diseases ,Neuroscience - Abstract
Supplemental material, sj-pdf-1-jcb-10.1177_0271678X221119760 for Transfer function analysis of dynamic cerebral autoregulation: a CARNet white paper 2022 update by Ronney B Panerai, Patrice Brassard, Joel S Burma, Pedro Castro, Jurgen AHR Claassen, Johannes J van Lieshout, Jia Liu, Samuel JE Lucas, Jatinder S Minhas, Georgios D Mitsis, Ricardo C Nogueira, Shigehiko Ogoh, Stephen J Payne, Caroline A Rickards, Andrew D Robertson, Gabriel D Rodrigues, Jonathan D Smirl David M Simpson in Journal of Cerebral Blood Flow & Metabolism
- Published
- 2022
- Full Text
- View/download PDF
50. Ink-jet-printed CuO nanoparticle-enhanced miniaturized paper-based electrochemical platform for hypochlorite sensing
- Author
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Ashirwad Ray, Jaligam Murali Mohan, Khairunnisa Amreen, Satish Kumar Dubey, Arshad Javed, R. N. Ponnalagu, and Sanket Goel
- Subjects
Materials Science (miscellaneous) ,Cell Biology ,Electrical and Electronic Engineering ,Physical and Theoretical Chemistry ,Atomic and Molecular Physics, and Optics ,Biotechnology - Published
- 2022
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