1. Cysteine-type cathepsins promote the effector phase of acute cutaneous delayed-type hypersensitivity reactions
- Author
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Hubert Kalbacher, Johannes Schwenck, Andreas Maurer, Kamran Ghoreschi, Philipp Knopf, Thomas Reinheckel, Michael Gütschow, Daniel Bukala, Irene Gonzalez Menendez, Martin Schaller, Leticia Quintanilla-Martinez, Kerstin Fuchs, Stefan Laufer, Martin Röcken, Roman Mehling, Dennis Haupt, Birgit Fehrenbacher, Manfred Kneilling, Christoph M. Griessinger, Natalie Mucha, Bernd J. Pichler, and Julia Holstein
- Subjects
Proteases ,Angiogenesis ,cathepsin B ,delayed-type hypersensitivity ,Medicine (miscellaneous) ,Inflammation ,Picryl Chloride ,Cathepsin B ,03 medical and health sciences ,0302 clinical medicine ,In vivo ,Catalytic Domain ,medicine ,Animals ,Humans ,Hypersensitivity, Delayed ,Protease Inhibitors ,Cysteine ,Pharmacology, Toxicology and Pharmaceutics (miscellaneous) ,Skin ,030304 developmental biology ,Cathepsin ,0303 health sciences ,Chemistry ,Optical Imaging ,Cathepsins ,Molecular biology ,Mice, Inbred C57BL ,030220 oncology & carcinogenesis ,Acute Disease ,Chronic Disease ,Female ,proteases ,Lymph ,medicine.symptom ,Ex vivo ,Research Paper - Abstract
Cysteine-type cathepsins such as cathepsin B are involved in various steps of inflammatory processes such as antigen processing and angiogenesis. Here, we uncovered the role of cysteine-type cathepsins in the effector phase of T cell-driven cutaneous delayed-type hypersensitivity reactions (DTHR) and the implication of this role on therapeutic cathepsin B-specific inhibition. Methods: Wild-type, cathepsin B-deficient (Ctsb-/-) and cathepsin Z-deficient (Ctsz-/-) mice were sensitized with 2,4,6-trinitrochlorobenzene (TNCB) on the abdomen and challenged with TNCB on the right ear to induce acute and chronic cutaneous DTHR. The severity of cutaneous DTHR was assessed by evaluating ear swelling responses and histopathology. We performed fluorescence microscopy on tissue from inflamed ears and lymph nodes of wild-type mice, as well as on biopsies from psoriasis patients, focusing on cathepsin B expression by T cells, B cells, macrophages, dendritic cells and NK cells. Cathepsin activity was determined noninvasively by optical imaging employing protease-activated substrate-like probes. Cathepsin expression and activity were validated ex vivo by covalent active site labeling of proteases and Western blotting. Results: Noninvasive in vivo optical imaging revealed strong cysteine-type cathepsin activity in inflamed ears and draining lymph nodes in acute and chronic cutaneous DTHR. In inflamed ears and draining lymph nodes, cathepsin B was expressed by neutrophils, dendritic cells, macrophages, B, T and natural killer (NK) cells. Similar expression patterns were found in psoriatic plaques of patients. The biochemical methods confirmed active cathepsin B in tissues of mice with cutaneous DTHR. Topically applied cathepsin B inhibitors significantly reduced ear swelling in acute but not chronic DTHR. Compared with wild-type mice, Ctsb-/- mice exhibited an enhanced ear swelling response during acute DTHR despite a lack of cathepsin B expression. Cathepsin Z, a protease closely related to cathepsin B, revealed compensatory expression in inflamed ears of Ctsb-/- mice, while cathepsin B expression was reciprocally elevated in Ctsz-/- mice. Conclusion: Cathepsin B is actively involved in the effector phase of acute cutaneous DTHR. Thus, topically applied cathepsin B inhibitors might effectively limit DTHR such as contact dermatitis or psoriasis. However, the cathepsin B and Z knockout mouse experiments suggested a complementary role for these two cysteine-type proteases.
- Published
- 2019