1. A new lysosome-targetable fluorescent probe with a large Stokes shift for detection of endogenous hydrogen polysulfides in living cells.
- Author
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Ren, Yanshen, Zhang, Lulu, Zhou, Ziyan, Luo, Yanjun, Wang, Suilou, Yuan, Shengtao, Gu, Yueqing, Xu, Yungen, and Zha, Xiaoming
- Subjects
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LYSOSOMES , *FLUORESCENT probes , *HYDROGEN sulfide , *CELL imaging , *PYRIDINE - Abstract
Abstract Hydrogen polysulfides (H 2 S n , n > 1) influence a variety of important biological functions and activities associated with hydrogen sulfide (H 2 S). The development of probes for rapid, selective, and sensitive detection of H 2 S n still remains a great challenge. Lysosome plays crucial roles in various physiological processes among living cells, which arouse high interest in detecting endogenous lysosome-targetable H 2 S n. To the best of our knowledge, there is no lysosome-targetable probe for H 2 S n has been reported. In this work, a new lysosome-targetable probe NIPY NF , based on the scaffold of imidazo [1,5-]pyridine, with a large Stokes shift (215 nm), low detection limit (84 nM), fast response time (6 min) and low cytotoxicity was designed and synthesized. Furthermore, NIPY NF was successfully applied into the cell imaging for detection of endogenous lysosome-targetable H 2 S n. Graphical abstract Image 1 Highlights • A novel lysosome-targetable probe NIPY NF was reported. • NIPY NF could discriminate H 2 S n over other analytes with high sensitivity, a low detection limit and a large Stokes shift. • NIPY NF could detect the endogenous H 2 S n in lysosome of living cells. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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